Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,984	+C	coding (1250/1299 nt)	trkH →	TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,983	1	.	C	99.6%	986.7 / ‑3.9	272	coding (1249/1299 nt)	trkH	TrkH
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (134/137); total (135/137)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 286 total aligned reads displayed.

TCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA  >  NC_000913/4031892‑4032070

tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑c                                                                                         <  1:5210797/93‑1 (MQ=255)

tCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTAAGTATGCTGGCGATTATCG‑c                                                                                         >  1:2654651/1‑93 (MQ=255)

   cgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑ccac                                                                                      <  2:4058004/93‑4 (MQ=255)

    gcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑ccacg                                                                                     <  2:672895/93‑5 (MQ=255)

    gcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑ccacg                                                                                     >  1:3596484/1‑89 (MQ=255)

       cTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGc                                                                                  >  2:4658518/1‑93 (MQ=255)

       cTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGc                                                                                  >  2:1642337/1‑93 (MQ=255)

          ccGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTg                                                                               <  1:5183291/93‑1 (MQ=255)

           cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTgg                                                                              >  1:1800249/1‑93 (MQ=255)

           cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTgg                                                                              >  2:3410479/1‑93 (MQ=255)

            ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             >  1:4601827/1‑93 (MQ=255)

            ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                             <  2:5644503/93‑1 (MQ=255)

              aaCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATg                                                                           >  1:1043221/1‑93 (MQ=255)

                cGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGAc                                                                         <  2:2624319/93‑1 (MQ=255)

                   aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      <  2:4244081/93‑1 (MQ=255)

                   aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  2:2077894/1‑93 (MQ=255)

                   aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  1:1017229/1‑93 (MQ=255)

                   aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                      >  1:967891/1‑93 (MQ=255)

                     ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttttt                                                                    >  2:4632408/1‑92 (MQ=255)

                      cTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCt                                                                   <  1:419410/93‑1 (MQ=255)

                       tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCtc                                                                  >  2:5767315/1‑92 (MQ=255)

                       tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTg                                                                  <  1:5418840/93‑1 (MQ=255)

                        cGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCtac                                                                 >  1:3701654/1‑91 (MQ=255)

                         gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGcc                                                                <  2:4092730/93‑1 (MQ=255)

                         gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGcc                                                                <  2:1533847/93‑1 (MQ=255)

                         gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGcc                                                                >  1:3523141/1‑93 (MQ=255)

                           aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCtt                                                              <  2:499852/93‑1 (MQ=255)

                            gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                             <  2:1375415/93‑1 (MQ=255)

                             ccGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTg                                                            <  2:3673923/93‑1 (MQ=255)

                              cGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGc                                                           <  1:5800163/93‑1 (MQ=255)

                               gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                          >  1:5723754/1‑93 (MQ=255)

                                  tGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCg                                                       <  1:5944315/93‑1 (MQ=255)

                                    gggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgt                                                     <  2:5187583/93‑1 (MQ=255)

                                      gATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttg                                                   <  2:977398/93‑1 (MQ=255)

                                       aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgt                                                  <  2:3872759/93‑1 (MQ=255)

                                       aTTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgt                                                  <  1:1451843/93‑1 (MQ=255)

                                        tttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgtt                                                 >  2:5992712/1‑93 (MQ=255)

                                        tttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgtt                                                 >  2:2681009/1‑93 (MQ=255)

                                         ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                                <  2:175761/93‑1 (MQ=255)

                                          tttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGc                                               >  2:3223697/1‑93 (MQ=255)

                                          tttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGc                                               >  1:2530015/1‑93 (MQ=255)

                                           ttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCg                                              >  1:3425299/1‑93 (MQ=255)

                                              tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                           >  2:3591515/1‑93 (MQ=255)

                                                 gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                        >  2:1054635/1‑93 (MQ=255)

                                                  ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       >  2:1213213/1‑93 (MQ=255)

                                                  ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       >  2:734127/1‑93 (MQ=255)

                                                  ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                       <  2:43684/93‑1 (MQ=255)

                                                    tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaaa                                     >  1:1983939/1‑92 (MQ=255)

                                                    tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                     <  1:1213638/93‑1 (MQ=255)

                                                    tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                     >  1:3105516/1‑93 (MQ=255)

                                                     aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                    >  1:3993309/1‑93 (MQ=255)

                                                     aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                    >  2:6181661/1‑93 (MQ=255)

                                                     aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGACGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                    <  1:5631984/93‑1 (MQ=255)

                                                      tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                   >  1:2998273/1‑93 (MQ=255)

                                                       gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                  <  1:6408449/93‑1 (MQ=255)

                                                       gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                  >  1:601147/1‑93 (MQ=255)

                                                        cATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                 >  1:1716188/1‑93 (MQ=255)

                                                         aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                <  1:4232194/93‑1 (MQ=255)

                                                          ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACCTGCGTGGGTGACTTTTCTGCCTTTGCGTCGGTTGTTCCGACATTGACTAACCTg                               >  1:993207/1‑93 (MQ=255)

                                                           tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                              <  1:3921647/93‑1 (MQ=255)

                                                           tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                              <  2:3290040/93‑1 (MQ=255)

                                                           tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                              <  1:2741999/93‑1 (MQ=255)

                                                           tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                              <  2:1433298/93‑1 (MQ=255)

                                                            tgtgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                             <  2:2406324/92‑1 (MQ=255)

                                                            ggTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                             >  1:1292915/1‑93 (MQ=255)

                                                             gtgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgggg                            <  2:5466326/93‑1 (MQ=255)

                                                             gtgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgggg                            <  2:3058417/93‑1 (MQ=255)

                                                             gtgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgggg                            >  2:1846085/1‑93 (MQ=255)

                                                               gtTTATTGTCAGTACGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGcc                          <  2:4569451/93‑1 (MQ=255)

                                                                tttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCa                         <  2:5928747/93‑1 (MQ=255)

                                                                 ttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAGCCTGTGGCCAg                        >  2:1464100/1‑93 (MQ=255)

                                                                 ttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAg                        <  2:317991/93‑1 (MQ=255)

                                                                 ttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTATGCCTTTGCGTCGGTTGCTGCGACATTGAATAACCTTGGGCCAg                        >  2:552785/1‑93 (MQ=255)

                                                                  tATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                       <  2:3512374/93‑1 (MQ=255)

                                                                   aTTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAggg                      >  1:6112254/1‑93 (MQ=255)

                                                                   aTTGTCAGTATGCTGGCGATTATCG‑CGACGGGCGTGGGTGACTTTTCTGCCTTTGCGTCGGGTGTTGCGACATTGAATAACCTGTGGCCAggg                      >  1:4535514/1‑93 (MQ=255)

                                                                    ttGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA‑CTGGGGCCAGGGCt                    <  1:744238/93‑1 (MQ=255)

                                                                     tGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCt                    <  1:218839/93‑1 (MQ=255)

                                                                     tGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCt                    <  2:5288448/93‑1 (MQ=255)

                                                                      gTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                   <  2:6273896/93‑1 (MQ=255)

                                                                       tCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCTTCGGTTGTTGCGACATTCAATAAACTGCGGCCAGGGCTTg                  >  2:2641400/1‑93 (MQ=255)

                                                                        cAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTgg                 <  2:4778977/93‑1 (MQ=255)

                                                                           tATGCTGGCGATTATCGCC‑ACGGGCGTTGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGGATAACCTGGGGCCAGGGCTTGGCGt              <  2:3023966/93‑1 (MQ=255)

                                                                           tATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGt              <  1:2028008/93‑1 (MQ=255)

                                                                           tATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGt              >  1:2879819/1‑93 (MQ=255)

                                                                             tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg            <  2:835580/93‑1 (MQ=255)

                                                                              gCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGt           <  1:4744103/93‑1 (MQ=255)

                                                                              gCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGt           <  2:216159/93‑1 (MQ=255)

                                                                               cTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgggt          >  2:4923800/1‑91 (MQ=255)

                                                                               cTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGtt          <  2:5250176/93‑1 (MQ=255)

                                                                                tGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTg         >  2:4449757/1‑93 (MQ=255)

                                                                                tGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTg         >  1:6171931/1‑93 (MQ=255)

                                                                                tGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTg         >  1:2859175/1‑93 (MQ=255)

                                                                                 ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc        >  2:2901625/1‑93 (MQ=255)

                                                                                 ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc        >  2:6606659/1‑93 (MQ=255)

                                                                                  gCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGACGGCTGGTGCGACATTGAATAACATGAGGCCAGCGCATGGAGTGGTTGCt       >  2:5697871/1‑93 (MQ=255)

                                                                                    gATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGa     >  2:1274337/1‑93 (MQ=255)

                                                                                    gATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGCGGTTGCTgt     >  1:5041763/1‑92 (MQ=255)

                                                                                     aTTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt    >  1:549429/1‑93 (MQ=255)

                                                                                       tATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATaa  <  2:5453742/93‑1 (MQ=255)

TCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA  >  NC_000913/4031892‑4032070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTAAGTATGCTGGCGATTATCG‑C                                                                                                >  SRR4302547.2708007/1‑94 (MQ=60)

 TCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑C                                                                                              <  SRR4302547.5318036/94‑1 (MQ=60)

   GCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Ccac                                                                                             >  SRR4302547.4652729/1‑91 (MQ=60)

    CGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACG                                                                                            >  SRR4302547.3669835/1‑94 (MQ=60)

     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGG                                                                                           >  SRR4302547.6649280/1‑94 (MQ=60)

     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGG                                                                                           >  SRR4302547.2331879/1‑94 (MQ=60)

     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGG                                                                                           >  SRR4302547.1317909/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGG                                                                                          <  SRR4302547.5127602/94‑1 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGG                                                                                          <  SRR4302547.6355920/94‑1 (MQ=60)

          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTG                                                                                      >  SRR4302547.1240491/1‑94 (MQ=60)

          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTG                                                                                      <  SRR4302547.2621393/94‑1 (MQ=60)

          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTG                                                                                      <  SRR4302547.1560262/94‑1 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGG                                                                                     >  SRR4302547.1835855/1‑94 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGG                                                                                     >  SRR4302547.3947823/1‑94 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGG                                                                                     <  SRR4302547.5289958/94‑1 (MQ=60)

            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGA                                                                                    >  SRR4302547.4697061/1‑94 (MQ=60)

            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGA                                                                                    <  SRR4302547.3204424/94‑1 (MQ=60)

             GGAACGTATCTTCGAAGCCGTTTGGGTATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAT                                                                                   <  SRR4302547.1058835/94‑1 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAT                                                                                   >  SRR4302547.4059097/1‑94 (MQ=60)

              GAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATG                                                                                  >  SRR4302547.1063809/1‑94 (MQ=60)

                ACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGAC                                                                                <  SRR4302547.2034974/94‑1 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACT                                                                               <  SRR4302547.3562651/94‑1 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACT                                                                               >  SRR4302547.4842983/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTT                                                                              >  SRR4302547.179623/1‑94 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGTTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTT                                                                             <  SRR4302547.5355697/94‑1 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTT                                                                             >  SRR4302547.986681/1‑94 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTT                                                                             <  SRR4302547.2185275/94‑1 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTT                                                                             >  SRR4302547.1037147/1‑94 (MQ=60)

                     TCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTC                                                                           >  SRR4302547.4987298/1‑94 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCT                                                                          >  SRR4302547.6758688/1‑94 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTG                                                                         <  SRR4302547.427188/94‑1 (MQ=60)

                        TCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGC                                                                        <  SRR4302547.5530930/94‑1 (MQ=60)

                        TCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTAC                                                                        >  SRR4302547.3777211/1‑94 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302547.3595050/1‑94 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302547.2203148/1‑94 (MQ=60)

                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCT                                                                      >  SRR4302547.3950060/1‑94 (MQ=60)

                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCT                                                                      >  SRR4302547.4676414/1‑94 (MQ=60)

                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCT                                                                      >  SRR4302547.5308774/1‑94 (MQ=60)

                          GAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCT                                                                      <  SRR4302547.6033884/94‑1 (MQ=60)

                             GCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   <  SRR4302547.973171/94‑1 (MQ=60)

                              CCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGC                                                                  >  SRR4302547.562466/1‑94 (MQ=60)

                              CCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGC                                                                  <  SRR4302547.2926026/94‑1 (MQ=60)

                              CCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGC                                                                  <  SRR4302547.4752965/94‑1 (MQ=60)

                               CGTTTGGGGGTTTTTCTCCGCCTATGCGTTGGTATTTATTGTCGGTATGCTGGCGTTAATCGCC‑ACGGGCGTGGATGACTTTTGTGCCTTTGCG                                                                 <  SRR4302547.427613/94‑1 (MQ=57)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302547.5841989/1‑94 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302547.3086700/1‑94 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302547.2990728/1‑94 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 <  SRR4302547.5920155/94‑1 (MQ=60)

                                 TTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               >  SRR4302547.2285382/1‑94 (MQ=60)

                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             <  SRR4302547.6067579/94‑1 (MQ=60)

                                     GGGGTTTTTCTCCGCCTATGCCTTGGTGTTTATTGGCAGTATGCTGGCGATTATCGCC‑ACGGGGGTGGATGACTTTTCTGCCTTTGCGGCGGTT                                                           <  SRR4302547.2468496/94‑1 (MQ=60)

                                      GGATTTTTCTCCGCCTATGCTTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          >  SRR4302547.5345530/1‑94 (MQ=60)

                                      GGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          >  SRR4302547.427361/1‑94 (MQ=60)

                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        <  SRR4302547.720701/94‑1 (MQ=60)

                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        <  SRR4302547.1480878/94‑1 (MQ=60)

                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTG                                                       <  SRR4302547.1780614/94‑1 (MQ=60)

                                          TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGC                                                      >  SRR4302547.2581146/1‑94 (MQ=60)

                                           TTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCG                                                     <  SRR4302547.3316531/94‑1 (MQ=60)

                                           TTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCG                                                     >  SRR4302547.3495055/1‑94 (MQ=60)

                                             TCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTTCGTCGGTTGTTGCGAC                                                   >  SRR4302547.5794640/1‑94 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  >  SRR4302547.6144004/1‑94 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  <  SRR4302547.1010051/94‑1 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               >  SRR4302547.2585344/1‑94 (MQ=60)

                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            >  SRR4302547.3167064/1‑94 (MQ=60)

                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAA                                            >  SRR4302547.2023337/1‑94 (MQ=60)

                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           >  SRR4302547.1987912/1‑94 (MQ=60)

                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           <  SRR4302547.1238121/94‑1 (MQ=60)

                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           >  SRR4302547.4074497/1‑94 (MQ=60)

                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGAATTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           >  SRR4302547.3717079/1‑94 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302547.3057906/1‑94 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGACGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302547.5748216/94‑1 (MQ=60)

                                                       TGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAC                                         >  SRR4302547.612274/1‑94 (MQ=60)

                                                        GCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        <  SRR4302547.6542093/94‑1 (MQ=60)

                                                        GCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        >  SRR4302547.1749938/1‑94 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302547.6469160/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302547.1324145/94‑1 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302547.4091824/1‑94 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      <  SRR4302547.4318717/94‑1 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACCTGCGTGGGTGACTTTTCTGCCTTTGCGTCGGTTGTTCCGACATTGACTAACCTG                                      >  SRR4302547.1012555/1‑94 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302547.5830289/94‑1 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302547.2797002/94‑1 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302547.6374033/94‑1 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    >  SRR4302547.1318940/1‑94 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302547.4001388/94‑1 (MQ=60)

                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   >  SRR4302547.2884368/1‑94 (MQ=60)

                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302547.4075032/94‑1 (MQ=60)

                                                             GGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   >  SRR4302547.5674450/1‑94 (MQ=60)

                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302547.3705196/1‑94 (MQ=60)

                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302547.3058018/1‑94 (MQ=60)

                                                               TGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCC                                 >  SRR4302547.5313699/1‑94 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                <  SRR4302547.140188/94‑1 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                <  SRR4302547.1148431/94‑1 (MQ=60)

                                                                 TTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAG                               <  SRR4302547.1066278/94‑1 (MQ=60)

                                                                  TTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              <  SRR4302547.461113/94‑1 (MQ=60)

                                                                   TATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGG                             >  SRR4302547.6239047/1‑94 (MQ=60)

                                                                   TATTGTCAGTATGCTGGCGATTATCG‑CGACGGGCGTGGGTGACTTTTCTGCCTTTGCGTCGGGTGTTGCGACATTGAATAACCTGTGGCCAGGG                             >  SRR4302547.4629250/1‑94 (MQ=60)

                                                                     TTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCT                           <  SRR4302547.1674792/94‑1 (MQ=60)

                                                                     TTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA‑CTGGGGCCAGGGCTT                          <  SRR4302547.758251/94‑1 (MQ=60)

                                                                      TGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          <  SRR4302547.2788488/94‑1 (MQ=60)

                                                                      TGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          <  SRR4302547.222958/94‑1 (MQ=60)

                                                                      TGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          >  SRR4302547.5188411/1‑94 (MQ=60)

                                                                      TGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          <  SRR4302547.5713893/94‑1 (MQ=60)

                                                                       gctcttccgatcttgacattcgcC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         >  SRR4302547.4407562/24‑94 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         >  SRR4302547.4528980/1‑94 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         <  SRR4302547.6089326/94‑1 (MQ=60)

                                                                          AGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      >  SRR4302547.1882959/1‑94 (MQ=60)

                                                                          AGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      <  SRR4302547.2919676/94‑1 (MQ=60)

                                                                           GTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGT                     >  SRR4302547.2937300/1‑94 (MQ=60)

                                                                           GTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGG                     >  SRR4302547.786528/1‑94 (MQ=60)

                                                                            TATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    >  SRR4302547.3643263/1‑94 (MQ=60)

                                                                            TATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    <  SRR4302547.2068392/94‑1 (MQ=60)

                                                                            TATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    >  SRR4302547.2859976/1‑94 (MQ=60)

                                                                             ATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   <  SRR4302547.3645660/94‑1 (MQ=60)

                                                                             ATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302547.6213968/1‑94 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302547.6294229/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302547.4842410/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302547.5546272/94‑1 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302547.2916296/1‑94 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                <  SRR4302547.4759210/94‑1 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                <  SRR4302547.5856115/94‑1 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                <  SRR4302547.5863190/94‑1 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302547.6300050/1‑94 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302547.2720629/1‑94 (MQ=60)

                                                                                  GGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              <  SRR4302547.6087633/94‑1 (MQ=60)

                                                                                  GGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              <  SRR4302547.5575103/94‑1 (MQ=60)

                                                                                  GGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGCGCTTGGCGTGGGTGCT              >  SRR4302547.2377028/1‑94 (MQ=60)

                                                                                   GCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTG             >  SRR4302547.2375110/1‑94 (MQ=60)

                                                                                    CGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGA            >  SRR4302547.3560688/1‑94 (MQ=60)

                                                                                    CGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGCGGTTGCTGT            >  SRR4302547.5145881/1‑94 (MQ=60)

                                                                                     GATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302547.559346/1‑94 (MQ=60)

                                                                                     GATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302547.6273123/1‑94 (MQ=60)

                                                                                      ATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA          >  SRR4302547.1268904/1‑94 (MQ=60)

                                                                                        TATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAAC        >  SRR4302547.6253569/1‑94 (MQ=60)

                                                                                        TATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAAC        >  SRR4302547.927633/1‑94 (MQ=60)

                                                                                         ATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACT       <  SRR4302547.1931754/94‑1 (MQ=60)

                                                                                           cgcC‑ACGGGCGTGGCTGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     >  SRR4302547.6733810/4‑94 (MQ=60)

                                                                                           cgcC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGGTGCTGATAACTTT     >  SRR4302547.547259/4‑94 (MQ=60)

                                                                                            gcC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    >  SRR4302547.4527805/3‑94 (MQ=60)

                                                                                            C‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATTACCTCGGGCCAGGGCTTGTCGTGGTTGCTGATCACTCTAC   >  SRR4302547.5876637/1‑94 (MQ=60)

                                                                                            C‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTAC   >  SRR4302547.2652950/1‑94 (MQ=60)

                                                                                              C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  SRR4302547.1080727/1‑94 (MQ=60)

                                                                                              C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  SRR4302547.2078148/1‑94 (MQ=60)

                                                                                              C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGGTGCTGATAACTTTATC  >  SRR4302547.7533/1‑94 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`