Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,091,351	C→T	G168D (GGC→GAC)	rhaD ←	rhamnulose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,091,351	0	C	T	99.5%	776.0 / NA	210	G168D (GGC→GAC)	rhaD	rhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (88/121); total (88/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

 cGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCtt                                                                                        >  2:6097730/1‑93 (MQ=255)

  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                       <  1:304926/93‑1 (MQ=255)

    tCGCCTGACCGACTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                     <  1:80621/93‑1 (MQ=255)

      gCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac                                                                                   <  2:3401253/93‑1 (MQ=255)

      gCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac                                                                                   >  2:6075402/1‑93 (MQ=255)

         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                <  1:1860516/93‑1 (MQ=255)

         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  2:348888/1‑93 (MQ=255)

         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                <  1:2770993/93‑1 (MQ=255)

          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               >  2:57422/1‑93 (MQ=255)

           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              <  2:2482193/93‑1 (MQ=255)

            gcGATTTCCTCCCTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGCGCCGTCTTCCCACAGTTgg                                                                             <  2:5303604/92‑1 (MQ=255)

             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTggg                                                                            >  2:1294251/1‑92 (MQ=255)

             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                            >  1:912156/1‑93 (MQ=255)

              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  1:946669/1‑93 (MQ=255)

               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          >  2:5819157/1‑93 (MQ=255)

               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          >  2:1820911/1‑93 (MQ=255)

               aTTTCGTCCGTGCCGGGCACCATCCACGGAAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          >  1:2884497/1‑93 (MQ=255)

                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         <  1:1034486/93‑1 (MQ=255)

                 ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAgg                                                                        >  2:844915/1‑92 (MQ=255)

                 ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                        <  2:4777389/93‑1 (MQ=255)

                   cGTCCGTGCGGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      <  1:574409/93‑1 (MQ=255)

                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      <  2:2251768/93‑1 (MQ=255)

                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      <  1:5882459/93‑1 (MQ=255)

                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      <  2:5247327/93‑1 (MQ=255)

                    gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  1:4395411/93‑1 (MQ=255)

                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    >  2:426744/1‑93 (MQ=255)

                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    <  2:795058/93‑1 (MQ=255)

                      ctgTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   <  1:3452272/91‑1 (MQ=255)

                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   <  2:3622605/93‑1 (MQ=255)

                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   <  1:5910413/93‑1 (MQ=255)

                         tGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcg                                                                <  2:3592615/93‑1 (MQ=255)

                           ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg                                                              >  1:919077/1‑93 (MQ=255)

                           ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg                                                              >  1:2266212/1‑93 (MQ=255)

                           ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg                                                              <  1:4716578/93‑1 (MQ=255)

                           ccGGGCACCATCCACGGCAAAATGCCAACGCCAGCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg                                                              <  2:5959798/93‑1 (MQ=255)

                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  1:5705718/93‑1 (MQ=255)

                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  1:3719999/93‑1 (MQ=255)

                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  1:1689797/93‑1 (MQ=255)

                             gggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt                                                            <  1:1167729/93‑1 (MQ=255)

                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgggt                                                          >  1:66526/1‑93 (MQ=255)

                                 cccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg                                                        <  1:4597603/92‑1 (MQ=255)

                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       >  2:3196949/1‑93 (MQ=255)

                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       <  1:87230/93‑1 (MQ=255)

                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     >  1:5234277/1‑93 (MQ=255)

                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  2:3759838/93‑1 (MQ=255)

                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  1:2541064/93‑1 (MQ=255)

                                      ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc                                                   >  1:615507/1‑93 (MQ=255)

                                       cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTTTCGTTTTCa                                                  <  1:4187927/93‑1 (MQ=255)

                                       cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCa                                                  >  1:526034/1‑93 (MQ=255)

                                        aCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCaa                                                 <  1:3197135/93‑1 (MQ=255)

                                          ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt                                               <  2:2964289/93‑1 (MQ=255)

                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              >  1:273481/1‑93 (MQ=255)

                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              <  1:3655821/93‑1 (MQ=255)

                                              aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt                                           <  1:6030010/93‑1 (MQ=255)

                                              aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt                                           <  2:3046438/93‑1 (MQ=255)

                                              aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt                                           <  2:218389/93‑1 (MQ=255)

                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          <  1:1157330/93‑1 (MQ=255)

                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         <  2:1900911/93‑1 (MQ=255)

                                                 tGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg                                        <  1:3061929/93‑1 (MQ=255)

                                                 tGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg                                        >  2:3824080/1‑93 (MQ=255)

                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt                                       <  2:1441873/93‑1 (MQ=255)

                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt                                       >  1:4921206/1‑93 (MQ=255)

                                                   ccAACGCCATCCGGGAATACCACCAGACACGCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg                                      <  1:4344474/93‑1 (MQ=255)

                                                    cAACGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  2:1657220/93‑1 (MQ=255)

                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   >  2:5904015/1‑93 (MQ=255)

                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  2:842241/93‑1 (MQ=255)

                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  2:5428774/93‑1 (MQ=255)

                                                       cGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg                                  <  2:3914510/93‑1 (MQ=255)

                                                       cGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg                                  <  2:1128828/93‑1 (MQ=255)

                                                        gCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGc                                 <  1:3786772/93‑1 (MQ=255)

                                                          cATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGa                               <  2:4911081/93‑1 (MQ=255)

                                                          cATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGa                               <  1:4010328/93‑1 (MQ=255)

                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             >  2:1349815/1‑93 (MQ=255)

                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  1:2351249/93‑1 (MQ=255)

                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:331822/93‑1 (MQ=255)

                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:3152028/93‑1 (MQ=255)

                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          >  2:4096902/1‑93 (MQ=255)

                                                                 gAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGtt                        >  1:4233198/1‑93 (MQ=255)

                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      <  1:1364189/93‑1 (MQ=255)

                                                                    tcccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     <  2:4461092/91‑1 (MQ=255)

                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggg                     >  1:2556893/1‑92 (MQ=255)

                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggg                     >  2:5287481/1‑92 (MQ=255)

                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGGTTTCAAGTACATAGGTGAGGGCGATCAGggtggt                     >  2:2651958/1‑93 (MQ=255)

                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 <  2:5828306/93‑1 (MQ=255)

                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               >  1:1701823/1‑93 (MQ=255)

                                                                           aGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              >  1:2588374/1‑93 (MQ=255)

                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             >  1:1245553/1‑93 (MQ=255)

                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             <  1:251908/93‑1 (MQ=255)

                                                                             acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCa            <  2:4142847/93‑1 (MQ=255)

                                                                              cacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAg           >  1:5049517/1‑93 (MQ=255)

                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         <  1:5622354/93‑1 (MQ=255)

                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        >  1:4887732/1‑93 (MQ=255)

                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        <  2:5171242/93‑1 (MQ=255)

                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       <  2:2419940/93‑1 (MQ=255)

                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTGTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       <  2:1888725/93‑1 (MQ=255)

                                                                                     tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGa    <  1:5975426/93‑1 (MQ=255)

                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   >  2:471443/1‑93 (MQ=255)

                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   >  2:212387/1‑93 (MQ=255)

                                                                                      gctggCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   <  1:2194873/93‑1 (MQ=255)

                                                                                       ctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATc  <  2:4440962/93‑1 (MQ=255)

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  NC_000913/4091258‑4091443

TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGT                                                                                              >  SRR4302532.2507157/1‑94 (MQ=60)

  TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                            >  SRR4302532.4777667/1‑94 (MQ=60)

  TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                            <  SRR4302532.6102527/94‑1 (MQ=60)

   GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                           <  SRR4302532.2734390/94‑1 (MQ=60)

   GCCGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                           <  SRR4302532.5436186/94‑1 (MQ=60)

     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         <  SRR4302532.315490/94‑1 (MQ=60)

     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         >  SRR4302532.2362492/1‑94 (MQ=60)

     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         >  SRR4302532.3286872/1‑94 (MQ=60)

       TCGCCTGACCGACTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                       <  SRR4302532.83479/94‑1 (MQ=60)

        CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                      >  SRR4302532.266087/1‑94 (MQ=60)

          CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    >  SRR4302532.2343120/1‑94 (MQ=60)

          CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCACACCCCATACGGGAATACCACCAGACACTGGGCGCTGTCTTCCCACAG                                                                                    >  SRR4302532.1356527/1‑94 (MQ=60)

            TGGCCGATTTCGTCCTTGCCGGGCACCATCCACGGGAAAATGCCAAGGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  <  SRR4302532.2682933/94‑1 (MQ=60)

            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  >  SRR4302532.394239/1‑94 (MQ=60)

            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  <  SRR4302532.2864034/94‑1 (MQ=60)

            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  <  SRR4302532.1922181/94‑1 (MQ=60)

            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  >  SRR4302532.6154141/1‑94 (MQ=60)

             gaccgcttacgtccgtgcagtgatcaatcccCGGCAAAAGGCGAACGCCATCCGGGAATACCACCAGACTCTCGGTGCTGTCTTCCCACAGTTG                                                                                 <  SRR4302532.5799165/63‑1 (MQ=58)

               CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                               >  SRR4302532.943324/1‑94 (MQ=60)

               CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                               >  SRR4302532.1517337/1‑94 (MQ=60)

                CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                              >  SRR4302532.979065/1‑94 (MQ=60)

                 GATTTCGTCCGTGCCGGGCACCATCCACGGAAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                             >  SRR4302532.2980893/1‑94 (MQ=60)

                  ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                            <  SRR4302532.2324058/94‑1 (MQ=60)

                   TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                           <  SRR4302532.1069414/94‑1 (MQ=60)

                    TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCACCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGcgga                                                                          >  SRR4302532.1633622/1‑90 (MQ=60)

                      CGTCCGTGCGGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                        <  SRR4302532.594443/94‑1 (MQ=60)

                      CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                        <  SRR4302532.6086206/94‑1 (MQ=60)

                       GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                       >  SRR4302532.1241353/1‑94 (MQ=60)

                       GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                       <  SRR4302532.4544939/94‑1 (MQ=60)

                         CTGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                     <  SRR4302532.3567896/94‑1 (MQ=60)

                         CCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                     <  SRR4302532.6115102/94‑1 (MQ=60)

                         CCGTCCCGGGCTCCCTCCACGGTAAAACGCCACCGTCACCCGGGAATGCCACCAGGCACTCTGTTCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                     <  SRR4302532.1221168/94‑1 (MQ=16)

                          CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                    >  SRR4302532.762293/1‑94 (MQ=60)

                          CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGAAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                    <  SRR4302532.2853214/94‑1 (MQ=60)

                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                 >  SRR4302532.2341426/1‑94 (MQ=60)

                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                 >  SRR4302532.950531/1‑94 (MQ=60)

                              CCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGG                                                                <  SRR4302532.4877501/94‑1 (MQ=60)

                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                               <  SRR4302532.5904653/94‑1 (MQ=60)

                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                               <  SRR4302532.3844886/94‑1 (MQ=60)

                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                               >  SRR4302532.3519334/1‑94 (MQ=60)

                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                               <  SRR4302532.1746863/94‑1 (MQ=60)

                                GGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                              <  SRR4302532.1206738/94‑1 (MQ=60)

                                CGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                              <  SRR4302532.1373036/94‑1 (MQ=60)

                                 GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGT                                                             >  SRR4302532.6213583/1‑94 (MQ=60)

                                 GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGGGT                                                             >  SRR4302532.68923/1‑94 (MQ=60)

                                  GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                            <  SRR4302532.4732922/94‑1 (MQ=60)

                                  GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                            <  SRR4302532.453435/94‑1 (MQ=60)

                                   CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                           >  SRR4302532.5315466/1‑94 (MQ=60)

                                    CCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                          <  SRR4302532.4754346/94‑1 (MQ=60)

                                    ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                          >  SRR4302532.3027562/1‑94 (MQ=60)

                                     CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                         <  SRR4302532.93271/94‑1 (MQ=60)

                                     CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                         <  SRR4302532.90307/94‑1 (MQ=60)

                                      CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                        >  SRR4302532.5414777/1‑94 (MQ=60)

                                       ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                       >  SRR4302532.1753557/1‑94 (MQ=60)

                                        TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                      >  SRR4302532.636965/1‑94 (MQ=60)

                                        TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                      <  SRR4302532.2625813/94‑1 (MQ=60)

                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                     >  SRR4302532.1203754/1‑94 (MQ=60)

                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                     >  SRR4302532.544391/1‑94 (MQ=60)

                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                     >  SRR4302532.2971504/1‑94 (MQ=60)

                                          CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTTTCGTTTTCAA                                                    <  SRR4302532.4328430/94‑1 (MQ=60)

                                          CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                    >  SRR4302532.3839730/1‑94 (MQ=60)

                                           CCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   <  SRR4302532.643829/94‑1 (MQ=60)

                                           ACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   <  SRR4302532.965955/94‑1 (MQ=60)

                                           ACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   <  SRR4302532.3303280/94‑1 (MQ=60)

                                            CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                  >  SRR4302532.4355004/1‑94 (MQ=60)

                                             GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                 >  SRR4302532.282952/1‑94 (MQ=60)

                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                <  SRR4302532.3778561/94‑1 (MQ=60)

                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                <  SRR4302532.320889/94‑1 (MQ=60)

                                                AAAATGCCAACGCCATCCGGGAATGCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              <  SRR4302532.5916578/94‑1 (MQ=60)

                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              >  SRR4302532.5082628/1‑94 (MQ=60)

                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              <  SRR4302532.4113025/94‑1 (MQ=60)

                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              >  SRR4302532.5332834/1‑94 (MQ=60)

                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              <  SRR4302532.3854623/94‑1 (MQ=60)

                                                 AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                             <  SRR4302532.6238261/94‑1 (MQ=60)

                                                  AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302532.1195998/94‑1 (MQ=60)

                                                  AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302532.4919529/94‑1 (MQ=60)

                                                  AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302532.2634072/94‑1 (MQ=60)

                                                   AGGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                           <  SRR4302532.376380/94‑1 (MQ=60)

                                                    TGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302532.1130659/94‑1 (MQ=60)

                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          >  SRR4302532.5090250/1‑94 (MQ=60)

                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302532.3163046/94‑1 (MQ=60)

                                                      CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                        >  SRR4302532.943583/1‑94 (MQ=60)

                                                      CCAACGCCATCCGGGAATACCACCAGACACGCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                        <  SRR4302532.4491258/94‑1 (MQ=60)

                                                       CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                       >  SRR4302532.2557799/1‑94 (MQ=60)

                                                         CCGCCATCCGGCAATCGCACCAGACACTCGGTGCTGTCTTCGCACAGTTGGCGAGTGAAGACAGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                     <  SRR4302532.295005/94‑1 (MQ=60)

                                                           GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                   <  SRR4302532.3914510/94‑1 (MQ=60)

                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                 <  SRR4302532.4145456/94‑1 (MQ=60)

                                                               TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGATGGCGATCA                               <  SRR4302532.6240553/94‑1 (MQ=60)

                                                               TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                               <  SRR4302532.2429697/94‑1 (MQ=60)

                                                                   GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           >  SRR4302532.4375328/1‑94 (MQ=60)

                                                                    GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                          <  SRR4302532.1983888/94‑1 (MQ=60)

                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                        <  SRR4302532.864399/94‑1 (MQ=60)

                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                        <  SRR4302532.1409878/94‑1 (MQ=60)

                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGG                        >  SRR4302532.2642178/1‑94 (MQ=60)

                                                                       TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                       <  SRR4302532.1895809/94‑1 (MQ=60)

                                                                       TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                       <  SRR4302532.969100/94‑1 (MQ=60)

                                                                        ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                      >  SRR4302532.2607606/1‑94 (MQ=60)

                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  >  SRR4302532.1759218/1‑94 (MQ=60)

                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTAGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  >  SRR4302532.4461036/1‑94 (MQ=60)

                                                                             CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                 >  SRR4302532.2674903/1‑94 (MQ=60)

                                                                              TGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                >  SRR4302532.1287224/1‑94 (MQ=60)

                                                                              AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                >  SRR4302532.5730455/1‑94 (MQ=60)

                                                                               GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA               <  SRR4302532.4733611/94‑1 (MQ=60)

                                                                               GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA               <  SRR4302532.260667/94‑1 (MQ=60)

                                                                                GCACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG              <  SRR4302532.6795/94‑1 (MQ=60)

                                                                                ACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG              >  SRR4302532.5222786/1‑94 (MQ=60)

                                                                                 CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT             >  SRR4302532.608855/1‑94 (MQ=60)

                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC           <  SRR4302532.5817454/94‑1 (MQ=60)

                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC           >  SRR4302532.5055716/1‑94 (MQ=60)

                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC           >  SRR4302532.1483706/1‑94 (MQ=60)

                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGA           >  SRR4302532.3158898/1‑94 (MQ=60)

                                                                                      GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG        >  SRR4302532.6014926/1‑94 (MQ=60)

                                                                                      GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG        <  SRR4302532.6029840/94‑1 (MQ=60)

                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA       <  SRR4302532.4188248/94‑1 (MQ=60)

                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT      >  SRR4302532.5138303/1‑94 (MQ=60)

                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT      <  SRR4302532.6181968/94‑1 (MQ=60)

                                                                                         GCTGGCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC     <  SRR4302532.2267986/94‑1 (MQ=60)

                                                                                          CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA    >  SRR4302532.3278021/1‑94 (MQ=60)

                                                                                           TGTCTTCCCACAGTTGGCGCGGGAAGACCGCGGTGTCGTGTTCAAGTACATAGGGCAGGGCGATCAGGCTGGTGGCGTGGCAGTGCATGCTCAC   >  SRR4302532.4237828/1‑94 (MQ=60)

                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302532.1351605/1‑94 (MQ=60)

                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCCAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  SRR4302532.3370848/1‑94 (MQ=60)

TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  NC_000913/4091258‑4091443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`