Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,3510CT99.7% 1236.5 / NA 324G168D (GGC→GAC) rhaDrhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (153/169);  minor base G (1/0);  total (154/170)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440
                                                                                          |                                                                                         
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                         >  1:3351185/1‑93 (MQ=255)
 cGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCtt                                                                                        >  1:4130811/1‑93 (MQ=255)
  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                       <  1:5052697/93‑1 (MQ=255)
  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                       >  2:3759076/1‑93 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  2:4977693/1‑93 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  1:6222896/1‑93 (MQ=255)
    tCGCCTGGCGGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                     <  1:2452596/93‑1 (MQ=255)
        cTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAg                                                                                 >  1:4395833/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  2:906669/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                <  1:1075254/93‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               <  1:753129/93‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               >  1:6006448/1‑93 (MQ=255)
           gCCGATTTCGTCGGTGCCGGGCACCATCCACGGCAAACTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              <  2:6174253/93‑1 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              <  2:1517076/93‑1 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              <  1:4792888/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             >  1:6655528/1‑93 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             <  2:5257983/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             >  2:4586917/1‑93 (MQ=255)
             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                            >  2:2469156/1‑93 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         >  2:3891672/1‑93 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGACATCCGGGAATACCACCCGACACTCGGTGCTGTCTTCCCACAGTTGGCCAg                                                                         >  1:5775244/1‑93 (MQ=255)
                  tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTg                                                                       <  2:3309364/93‑1 (MQ=255)
                  tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTg                                                                       >  1:389100/1‑93 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      >  1:5904654/1‑93 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    >  1:3520523/1‑93 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    <  1:2906627/93‑1 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    >  2:3237206/1‑93 (MQ=255)
                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   >  1:639622/1‑93 (MQ=255)
                       cGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACTACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc                                                                  >  2:2077332/1‑93 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 <  2:4363046/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 <  2:6584460/93‑1 (MQ=255)
                         tGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGCGTGAAGACcg                                                                >  2:2549890/1‑93 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:2197915/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               >  2:4150739/1‑93 (MQ=255)
                            cGGCCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  1:3018422/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          >  2:4518563/1‑93 (MQ=255)
                                cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTc                                                         >  2:1227929/1‑93 (MQ=255)
                                 aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg                                                        >  2:515201/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      <  1:905858/93‑1 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      >  2:5802699/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      >  1:3266422/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      <  1:398634/93‑1 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     <  2:3110988/93‑1 (MQ=255)
                                      ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc                                                   <  1:6499557/93‑1 (MQ=255)
                                        aCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCaa                                                 <  2:3350766/93‑1 (MQ=255)
                                         cGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAg                                                <  1:4669424/93‑1 (MQ=255)
                                          ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt                                               >  2:2694381/1‑93 (MQ=255)
                                          ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt                                               <  1:5887063/93‑1 (MQ=255)
                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              <  1:2770143/93‑1 (MQ=255)
                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              >  2:5672495/1‑93 (MQ=255)
                                           gCAAAATGCCAACGCAATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              <  1:5164359/93‑1 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            >  2:354247/1‑93 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            >  2:1704442/1‑93 (MQ=255)
                                              aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt                                           >  1:3182647/1‑93 (MQ=255)
                                              aaaTGCCAACGCCATCCGGGAATAACACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCTAGTACAt                                           >  1:3392975/1‑93 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         >  2:4477822/1‑93 (MQ=255)
                                                 tGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg                                        <  2:782276/93‑1 (MQ=255)
                                                 tGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg                                        <  1:4992216/93‑1 (MQ=255)
                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt                                       <  2:6483291/93‑1 (MQ=255)
                                                   ccAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg                                      <  1:4142265/93‑1 (MQ=255)
                                                     aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAg                                    >  1:3044304/1‑93 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                <  2:68379/93‑1 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                <  1:6365494/93‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  1:5374189/93‑1 (MQ=255)
                                                             ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCa                            <  1:99796/93‑1 (MQ=255)
                                                              cGGGAATACCACCAGACCCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:3506230/93‑1 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           >  1:3452510/1‑93 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          >  1:1519633/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:5850687/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:4580488/93‑1 (MQ=255)
                                                                 gAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGtt                        <  2:5439609/93‑1 (MQ=255)
                                                                     accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg                    <  1:2103682/93‑1 (MQ=255)
                                                                     accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg                    >  2:1800795/1‑93 (MQ=255)
                                                                     accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACACAGGTGAGGGCGATCAGGTtggtg                    >  2:4829137/1‑93 (MQ=255)
                                                                      ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtgg                   >  2:2859092/1‑93 (MQ=255)
                                                                      ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtgg                   >  2:2363281/1‑93 (MQ=255)
                                                                       caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggc                  >  1:5609641/1‑93 (MQ=255)
                                                                       caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggc                  <  1:4828989/93‑1 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  1:3697834/1‑93 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgt                <  1:1323018/93‑1 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               >  1:5413117/1‑93 (MQ=255)
                                                                           aGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              <  1:1675426/93‑1 (MQ=255)
                                                                           aGACACTCGGTGCTGTCTCCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              <  2:2961198/93‑1 (MQ=255)
                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             <  1:4328567/93‑1 (MQ=255)
                                                                             acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCa            >  2:1347085/1‑93 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          >  2:6047069/1‑93 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         <  2:5542529/93‑1 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         >  1:2840557/1‑93 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         <  1:2698063/93‑1 (MQ=255)
                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        >  1:2295992/1‑93 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       >  2:383482/1‑93 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       >  2:1245974/1‑93 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       <  1:2328698/93‑1 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       >  1:4459302/1‑93 (MQ=255)
                                                                                   ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAt      >  1:5558023/1‑93 (MQ=255)
                                                                                     tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATgt    >  2:1629136/1‑92 (MQ=255)
                                                                                     tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGa    <  2:2222683/93‑1 (MQ=255)
                                                                                     tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGa    <  1:1851620/93‑1 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   <  1:5716268/93‑1 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   <  1:2275582/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  NC_000913/4091258‑4091443
                                                                                             |                                                                                            
TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGT                                                                                              >  SRR4302547.3183411/1‑94 (MQ=60)
 GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                             >  SRR4302547.2110460/1‑94 (MQ=60)
 GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                             <  SRR4302547.1950088/94‑1 (MQ=60)
  TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                            >  SRR4302547.3419140/1‑94 (MQ=60)
   GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                           <  SRR4302547.234529/94‑1 (MQ=60)
   GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                           >  SRR4302547.4214853/1‑94 (MQ=60)
   GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                           >  SRR4302547.2960135/1‑94 (MQ=60)
    GGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTC                                                                                          <  SRR4302547.4926809/94‑1 (MQ=60)
    CGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTC                                                                                          <  SRR4302547.4790584/94‑1 (MQ=60)
     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         >  SRR4302547.6352130/1‑94 (MQ=60)
     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         <  SRR4302547.5157010/94‑1 (MQ=60)
     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         <  SRR4302547.5217334/94‑1 (MQ=60)
      GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                        <  SRR4302547.5565996/94‑1 (MQ=60)
       TCGCCTGGCGGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                       <  SRR4302547.2502163/94‑1 (MQ=60)
       TCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                       >  SRR4302547.380987/1‑94 (MQ=60)
        CTCCTGTCCGATTGCGTCCGTGCCGGGCACCATCAACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                      <  SRR4302547.4766495/94‑1 (MQ=60)
        CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGCCACTCGGTGCCGCCTTCCCAC                                                                                      >  SRR4302547.5048731/1‑94 (MQ=60)
        CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                      >  SRR4302547.4664872/1‑94 (MQ=60)
        CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                      >  SRR4302547.2978243/1‑94 (MQ=60)
         GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                     >  SRR4302547.5061223/1‑94 (MQ=60)
         GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGCTGCTGTCTTCCCACA                                                                                     >  SRR4302547.2728635/1‑94 (MQ=60)
          CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCATAG                                                                                    <  SRR4302547.6687645/94‑1 (MQ=60)
          CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    >  SRR4302547.4486326/1‑94 (MQ=60)
          CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    >  SRR4302547.2467147/1‑94 (MQ=60)
           CTGGCCGTTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACAACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302547.2320964/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302547.3789122/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302547.3425423/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302547.1811501/1‑94 (MQ=60)
           CTGGCCGATCTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   <  SRR4302547.2905353/94‑1 (MQ=60)
            TGTCCGATTTTGTCCGTGCCGGCCACAATCCACGGCAAAATGCCAACGCCATCCGGTAATACCACAAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  <  SRR4302547.4250616/94‑1 (MQ=60)
            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  >  SRR4302547.6131120/1‑94 (MQ=60)
            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  <  SRR4302547.1096715/94‑1 (MQ=60)
             GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                 <  SRR4302547.767329/94‑1 (MQ=60)
              GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCGACAGTTGG                                                                                >  SRR4302547.3386498/1‑94 (MQ=60)
              GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                >  SRR4302547.5682885/1‑94 (MQ=60)
              GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                >  SRR4302547.6794470/1‑94 (MQ=60)
              GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                <  SRR4302547.4892201/94‑1 (MQ=60)
               CTGATTTCGTCCGTGCCGGGCACAATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                               <  SRR4302547.3511755/94‑1 (MQ=60)
                CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                              >  SRR4302547.5647672/1‑94 (MQ=60)
                  ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                            <  SRR4302547.1710956/94‑1 (MQ=60)
                  ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGACATCCGGGAATACCACCCGACACTCGGTGCTGTCTTCCCACAGTTGGCCAG                                                                            >  SRR4302547.5894697/1‑94 (MQ=60)
                   TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                           <  SRR4302547.3588914/94‑1 (MQ=60)
                   TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                           >  SRR4302547.6521113/1‑94 (MQ=60)
                   TTCCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                           <  SRR4302547.6128920/94‑1 (MQ=60)
                    TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                          >  SRR4302547.396316/1‑94 (MQ=60)
                    TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                          <  SRR4302547.6132445/94‑1 (MQ=60)
                     TCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGA                                                                         >  SRR4302547.6026938/1‑94 (MQ=60)
                      CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                        <  SRR4302547.6543695/94‑1 (MQ=60)
                       GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                       >  SRR4302547.3592386/1‑94 (MQ=60)
                        TCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGA                                                                      >  SRR4302547.3100949/1‑94 (MQ=60)
                        TCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGA                                                                      <  SRR4302547.2964582/94‑1 (MQ=60)
                        TCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGA                                                                      >  SRR4302547.651419/1‑94 (MQ=60)
                         CCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                     <  SRR4302547.6319465/94‑1 (MQ=60)
                          CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                    <  SRR4302547.4679316/94‑1 (MQ=60)
                          CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                    <  SRR4302547.2271494/94‑1 (MQ=60)
                          CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGGCTTCCCACAGTTGGCGAGTGAAGACC                                                                    >  SRR4302547.4341502/1‑94 (MQ=60)
                            TGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGC                                                                  <  SRR4302547.5998191/94‑1 (MQ=60)
                            TGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGC                                                                  <  SRR4302547.2341149/94‑1 (MQ=60)
                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                 <  SRR4302547.4223246/94‑1 (MQ=60)
                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                 <  SRR4302547.2242068/94‑1 (MQ=60)
                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                 <  SRR4302547.2338593/94‑1 (MQ=60)
                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                               >  SRR4302547.6081258/1‑94 (MQ=60)
                               CGGCCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                               <  SRR4302547.3078444/94‑1 (MQ=60)
                                 GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGT                                                             <  SRR4302547.1299854/94‑1 (MQ=60)
                                 GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGT                                                             >  SRR4302547.5994134/1‑94 (MQ=60)
                                 GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGGGT                                                             >  SRR4302547.5174446/1‑94 (MQ=60)
                                   CGCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                           <  SRR4302547.513462/94‑1 (MQ=60)
                                   CACCATCCACGGCAAAATGCCAACGCTATCCGGGAATACCACCAGACTCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                           <  SRR4302547.1591970/94‑1 (MQ=60)
                                    ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                          <  SRR4302547.3078866/94‑1 (MQ=60)
                                    ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                          >  SRR4302547.2039238/1‑94 (MQ=60)
                                     CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                         >  SRR4302547.3331775/1‑94 (MQ=60)
                                     CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                         <  SRR4302547.4666184/94‑1 (MQ=60)
                                      CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                        <  SRR4302547.406049/94‑1 (MQ=60)
                                      CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                        <  SRR4302547.923313/94‑1 (MQ=60)
                                      CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                        <  SRR4302547.2018618/94‑1 (MQ=60)
                                       ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                       >  SRR4302547.2537923/1‑94 (MQ=60)
                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                     <  SRR4302547.6634835/94‑1 (MQ=60)
                                          CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGATGACCGCGGTGTCGTTTTCAA                                                    >  SRR4302547.3993571/1‑94 (MQ=60)
                                           ACGGCAAAATGCCCTCACCATCCGGGAATTCCCCCAGACCCTCGGTCCTGTCTTCCAACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   <  SRR4302547.5575409/94‑1 (MQ=53)
                                           ACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   <  SRR4302547.4242769/94‑1 (MQ=60)
                                           ACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   <  SRR4302547.5821630/94‑1 (MQ=60)
                                            CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                  <  SRR4302547.4766123/94‑1 (MQ=60)
                                            CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                  >  SRR4302547.5615110/1‑94 (MQ=60)
                                             GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                 <  SRR4302547.6008945/94‑1 (MQ=60)
                                             GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                 >  SRR4302547.2775033/1‑94 (MQ=60)
                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                <  SRR4302547.2825643/94‑1 (MQ=60)
                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                <  SRR4302547.1653586/94‑1 (MQ=60)
                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                >  SRR4302547.169849/1‑94 (MQ=60)
                                              GCAAAATGCCAACGCAATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                <  SRR4302547.5270689/94‑1 (MQ=60)
                                               CAAAATGCCAGCGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                               <  SRR4302547.3334455/94‑1 (MQ=60)
                                               CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                               >  SRR4302547.4531976/1‑94 (MQ=60)
                                               CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                               >  SRR4302547.2784525/1‑94 (MQ=60)
                                               CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                               >  SRR4302547.3509700/1‑94 (MQ=60)
                                               CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                               >  SRR4302547.3147318/1‑94 (MQ=60)
                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              >  SRR4302547.3246070/1‑94 (MQ=60)
                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              <  SRR4302547.6047542/94‑1 (MQ=60)
                                                AAAATGCCAACGCCATCCGGGAATAACACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCTAGTACAT                                              >  SRR4302547.3462005/1‑94 (MQ=60)
                                                 AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                             <  SRR4302547.2872506/94‑1 (MQ=60)
                                                 AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                             >  SRR4302547.2813487/1‑94 (MQ=60)
                                                 AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                             <  SRR4302547.4239261/94‑1 (MQ=60)
                                                  AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            >  SRR4302547.4832847/1‑94 (MQ=60)
                                                  AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302547.4451214/94‑1 (MQ=60)
                                                   ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                           <  SRR4302547.1297573/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302547.3487727/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302547.5095405/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302547.2933354/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302547.1384436/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                          <  SRR4302547.3446585/94‑1 (MQ=60)
                                                     GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                         <  SRR4302547.6524707/94‑1 (MQ=60)
                                                      CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                        <  SRR4302547.4226561/94‑1 (MQ=60)
                                                       CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                       >  SRR4302547.3104887/1‑94 (MQ=60)
                                                        AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCTCAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                      <  SRR4302547.5913429/94‑1 (MQ=60)
                                                        AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                      <  SRR4302547.5497777/94‑1 (MQ=60)
                                                        AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                      <  SRR4302547.4184293/94‑1 (MQ=60)
                                                         ACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                     <  SRR4302547.3238556/94‑1 (MQ=60)
                                                          CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                    <  SRR4302547.827069/94‑1 (MQ=60)
                                                           GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                   >  SRR4302547.4145525/1‑94 (MQ=60)
                                                           GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                   >  SRR4302547.4655044/1‑94 (MQ=60)
                                                           GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                   >  SRR4302547.2181903/1‑94 (MQ=60)
                                                           GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                   >  SRR4302547.3646750/1‑94 (MQ=60)
                                                            CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                  <  SRR4302547.6498292/94‑1 (MQ=60)
                                                            CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                  >  SRR4302547.5066375/1‑94 (MQ=60)
                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                 <  SRR4302547.717662/94‑1 (MQ=60)
                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                 <  SRR4302547.325468/94‑1 (MQ=60)
                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                 >  SRR4302547.6699659/1‑94 (MQ=60)
                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                 <  SRR4302547.2489706/94‑1 (MQ=60)
                                                              ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                <  SRR4302547.3365657/94‑1 (MQ=60)
                                                               TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                               <  SRR4302547.5485101/94‑1 (MQ=60)
                                                               TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                               <  SRR4302547.2472874/94‑1 (MQ=60)
                                                                CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                              >  SRR4302547.4174266/1‑94 (MQ=60)
                                                                CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                              <  SRR4302547.5443332/94‑1 (MQ=60)
                                                                CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                              <  SRR4302547.101636/94‑1 (MQ=60)
                                                                CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                              >  SRR4302547.3522898/1‑94 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                             >  SRR4302547.871572/1‑94 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                             <  SRR4302547.3684124/94‑1 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                             >  SRR4302547.1549900/1‑94 (MQ=60)
                                                                  GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                            >  SRR4302547.4472347/1‑94 (MQ=60)
                                                                  GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                            <  SRR4302547.6542053/94‑1 (MQ=60)
                                                                   GGAATTCCACCAGACCCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           <  SRR4302547.5486592/94‑1 (MQ=60)
                                                                   GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           <  SRR4302547.4675285/94‑1 (MQ=60)
                                                                   GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           <  SRR4302547.5690530/94‑1 (MQ=60)
                                                                   GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           <  SRR4302547.5971715/94‑1 (MQ=60)
                                                                    GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                          >  SRR4302547.5888929/1‑94 (MQ=60)
                                                                    GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                          >  SRR4302547.327610/1‑94 (MQ=60)
                                                                    GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                          >  SRR4302547.1351867/1‑94 (MQ=60)
                                                                    GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                          <  SRR4302547.6368924/94‑1 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                        <  SRR4302547.817604/94‑1 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                        <  SRR4302547.5922745/94‑1 (MQ=60)
                                                                        ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                      <  SRR4302547.2145606/94‑1 (MQ=60)
                                                                        ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                      >  SRR4302547.2836768/1‑94 (MQ=60)
                                                                        ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGCTGGTGG                      >  SRR4302547.3076575/1‑94 (MQ=60)
                                                                         CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGC                     >  SRR4302547.5725395/1‑94 (MQ=60)
                                                                         CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGC                     >  SRR4302547.2342659/1‑94 (MQ=60)
                                                                         CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGC                     <  SRR4302547.3199644/94‑1 (MQ=60)
                                                                          CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                    >  SRR4302547.6428243/1‑94 (MQ=60)
                                                                          CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                    <  SRR4302547.4929109/94‑1 (MQ=60)
                                                                          CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                    >  SRR4302547.3773306/1‑94 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  <  SRR4302547.3872396/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  <  SRR4302547.1349606/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  <  SRR4302547.4538408/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  <  SRR4302547.5976093/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  >  SRR4302547.5525084/1‑94 (MQ=60)
                                                                             CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                 <  SRR4302547.5865317/94‑1 (MQ=60)
                                                                             CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                 >  SRR4302547.6012957/1‑94 (MQ=60)
                                                                              AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                <  SRR4302547.1708429/94‑1 (MQ=60)
                                                                              AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                >  SRR4302547.575301/1‑94 (MQ=60)
                                                                              AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                >  SRR4302547.764823/1‑94 (MQ=60)
                                                                               GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA               <  SRR4302547.4417646/94‑1 (MQ=60)
                                                                                ACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG              <  SRR4302547.526439/94‑1 (MQ=60)
                                                                                ACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG              <  SRR4302547.3979323/94‑1 (MQ=60)
                                                                                 CGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT             <  SRR4302547.6023090/94‑1 (MQ=60)
                                                                                 CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT             >  SRR4302547.5390201/1‑94 (MQ=60)
                                                                                  CCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG            <  SRR4302547.4216561/94‑1 (MQ=60)
                                                                                  ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG            >  SRR4302547.2897322/1‑94 (MQ=60)
                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC           <  SRR4302547.2752227/94‑1 (MQ=60)
                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC           >  SRR4302547.2342515/1‑94 (MQ=60)
                                                                                    TCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCA          >  SRR4302547.4551475/1‑94 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT         <  SRR4302547.6763032/94‑1 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT         >  SRR4302547.5672669/1‑94 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT         <  SRR4302547.4713662/94‑1 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT         <  SRR4302547.2375896/94‑1 (MQ=60)
                                                                                      GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGGGGCGTGGCAGTGCATG        >  SRR4302547.5740759/1‑94 (MQ=60)
                                                                                      GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGGGGCGTGGCAGTGCATG        >  SRR4302547.3528743/1‑94 (MQ=60)
                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA       >  SRR4302547.4893647/1‑94 (MQ=60)
                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA       >  SRR4302547.508725/1‑94 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT      >  SRR4302547.100259/1‑94 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT      <  SRR4302547.2305485/94‑1 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT      <  SRR4302547.1888209/94‑1 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT      >  SRR4302547.2009628/1‑94 (MQ=60)
                                                                                         GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC     <  SRR4302547.6465776/94‑1 (MQ=60)
                                                                                         GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC     <  SRR4302547.2321603/94‑1 (MQ=60)
                                                                                         GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC     <  SRR4302547.5834321/94‑1 (MQ=60)
                                                                                         GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC     <  SRR4302547.2206251/94‑1 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302547.4922700/1‑94 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302547.6393418/1‑94 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302547.382461/1‑94 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATTACC  >  SRR4302547.3865832/1‑94 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  SRR4302547.2825762/1‑94 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  <  SRR4302547.113209/94‑1 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  <  SRR4302547.5997795/94‑1 (MQ=60)
                                                                                             |                                                                                            
TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  NC_000913/4091258‑4091443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑