Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,133,368	G→T	E652* (GAG→TAG)	katG →	catalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,133,368	0	G	T	98.4%	939.5 / 4.4	256	E652* (GAG→TAG)	katG	catalase
Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (136/116); total (139/117)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 261 total aligned reads displayed.

CAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGAT  >  NC_000913/4133277‑4133457

 aaCTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAg                                                                                         >  1:5624270/1‑93 (MQ=255)

   cTTCGATGGCAGCAAAAACGGCGTCTTCACTGACTGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTg                                                                                       >  2:2658772/1‑93 (MQ=255)

   cTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTg                                                                                       >  1:574544/1‑93 (MQ=255)

   cTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTg                                                                                       >  1:2497388/1‑93 (MQ=255)

    ttCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTgg                                                                                      <  2:5708378/93‑1 (MQ=255)

     tCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGGTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTGGTGGa                                                                                     >  2:2072032/1‑93 (MQ=255)

        aTGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAg                                                                                  <  1:1478342/93‑1 (MQ=255)

           gcagcaAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGcga                                                                               <  2:2380522/93‑1 (MQ=255)

            cagcaAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGcgac                                                                              <  1:5692120/93‑1 (MQ=255)

            cagcaAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGcgac                                                                              <  2:296103/93‑1 (MQ=255)

              gcaAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGcgaccg                                                                            >  1:710180/1‑93 (MQ=255)

               caAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGcgaccga                                                                           <  2:2750272/93‑1 (MQ=255)

                 aaaaCGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACcgacg                                                                         >  1:4783324/1‑93 (MQ=255)

                 aaaaCGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACcgacg                                                                         >  1:636233/1‑93 (MQ=255)

                 aaaaCGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACcgacg                                                                         >  2:2970975/1‑93 (MQ=255)

                   aaCGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGaa                                                                       <  2:1435999/93‑1 (MQ=255)

                   aaCGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGaa                                                                       >  2:2867598/1‑93 (MQ=255)

                     cGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATc                                                                     <  2:1857795/93‑1 (MQ=255)

                      ggCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCg                                                                    >  2:5329706/1‑93 (MQ=255)

                         gTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGCTACTAACGGAAAGCGACAGACGCATCGaaa                                                                 >  1:1586173/1‑93 (MQ=255)

                          tCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAag                                                                >  2:3622276/1‑93 (MQ=255)

                          tCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAag                                                                >  2:1328187/1‑93 (MQ=255)

                          tCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAag                                                                >  1:3223574/1‑93 (MQ=255)

                           cTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAaga                                                               >  2:892893/1‑93 (MQ=255)

                             tCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGc                                                             <  2:3375867/93‑1 (MQ=255)

                             tCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGc                                                             <  1:4440278/93‑1 (MQ=255)

                              cACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCt                                                            >  1:5137230/1‑93 (MQ=255)

                               aCTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTg                                                           >  1:2453270/1‑93 (MQ=255)

                               aCTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTg                                                           >  1:4343856/1‑93 (MQ=255)

                                cTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGt                                                          <  2:2515469/93‑1 (MQ=255)

                                cTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGt                                                          <  1:6068130/93‑1 (MQ=255)

                                 tGACGGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGtt                                                         <  2:3131253/93‑1 (MQ=255)

                                  gACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTc                                                        <  1:5414168/93‑1 (MQ=255)

                                  gACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTc                                                        >  1:5224713/1‑93 (MQ=255)

                                   aCCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCg                                                       <  1:2920366/93‑1 (MQ=255)

                                    ccGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGa                                                      >  1:496117/1‑93 (MQ=255)

                                     cgcgTTGTCGTATTGATCAATGTCGTCTTCGTGAACTTGCTGGATATGCGTTACTAGGGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGaa                                                     <  1:3811444/93‑1 (MQ=255)

                                     cgcgTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGTCCGACGAATCGAAAGAGCTGTTCGaa                                                     <  2:4019212/93‑1 (MQ=255)

                                     cgcgTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGaa                                                     >  1:1781934/1‑93 (MQ=255)

                                      gcgTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAg                                                    <  1:3978871/93‑1 (MQ=255)

                                      gcgTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAg                                                    >  1:1212500/1‑93 (MQ=255)

                                      gcgTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATACGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAg                                                    <  2:4215847/93‑1 (MQ=255)

                                          tGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccg                                                >  2:350414/1‑93 (MQ=255)

                                          tGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccg                                                >  2:165104/1‑93 (MQ=255)

                                          tGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccg                                                <  2:598676/93‑1 (MQ=255)

                                          tGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccg                                                >  2:1073783/1‑93 (MQ=255)

                                          tGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccg                                                <  1:4001292/93‑1 (MQ=255)

                                            gCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtg                                              <  1:5811054/93‑1 (MQ=255)

                                              gTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgac                                            <  2:5877479/93‑1 (MQ=255)

                                               tATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgacc                                           >  2:730780/1‑93 (MQ=255)

                                               tATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgacc                                           >  2:2447893/1‑93 (MQ=255)

                                                 ttGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgaccgt                                         >  2:6128441/1‑93 (MQ=255)

                                                 ttGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgaccgt                                         <  1:1951338/93‑1 (MQ=255)

                                                  tGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgaccgtg                                        >  1:1002448/1‑93 (MQ=255)

                                                    aGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGaa                                      >  2:5538548/1‑93 (MQ=255)

                                                     gCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAATGAGCTGTTCGAAGGCCGTGACCGTGaaa                                     >  2:4349216/1‑93 (MQ=255)

                                                     gCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGaaa                                     <  1:3193562/93‑1 (MQ=255)

                                                       caTGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAAcc                                   <  2:4767683/92‑1 (MQ=255)

                                                       aaTGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAAcg                                   >  1:6080504/1‑92 (MQ=255)

                                                       aaTGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAAcc                                   >  2:5404573/1‑93 (MQ=255)

                                                        aTGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCg                                  >  1:1025432/1‑93 (MQ=255)

                                                        aTGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCg                                  >  2:3865927/1‑93 (MQ=255)

                                                         tGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCgg                                 <  1:2505853/93‑1 (MQ=255)

                                                            cttcttCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGa                              >  2:562518/1‑93 (MQ=255)

                                                             ttcttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGaa                             >  1:3580395/1‑93 (MQ=255)

                                                             ttcttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGaa                             >  2:1781071/1‑93 (MQ=255)

                                                             ttcttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGaa                             >  1:2842199/1‑93 (MQ=255)

                                                              tcttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAg                            >  1:467368/1‑93 (MQ=255)

                                                              tcttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAg                            >  1:3698318/1‑93 (MQ=255)

                                                               cttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGt                           <  2:5010907/93‑1 (MQ=255)

                                                               cttcGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGt                           <  1:2565554/93‑1 (MQ=255)

                                                                ttcGTGAACTTGCTGGATATGCGTTACTTGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTg                          >  2:5777060/1‑93 (MQ=255)

                                                                     gAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAAtt                     <  1:210707/93‑1 (MQ=255)

                                                                       agttgttgGATATGCGTTAATAGTGGAAAGCGAACGACGAATCGAAAGAGCTGTTCGAAGGCAGTGACCGTGAAACCGGCGAAGTGAAATTTa                   <  2:2563032/91‑1 (MQ=255)

                                                                       aCTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAATTGGCGAAGTGAAATTTa                   >  2:5499364/1‑93 (MQ=255)

                                                                       aCTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTa                   <  1:4527879/93‑1 (MQ=255)

                                                                         ttGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACg                 >  2:2033618/1‑93 (MQ=255)

                                                                          tGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACgg                >  2:5652497/1‑93 (MQ=255)

                                                                           gcgggATATGCGTTACGAGTGGAGAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACAGGCGAAGTGAAATTTACGGc               <  1:4219508/90‑1 (MQ=255)

                                                                           gCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGc               >  2:2201266/1‑93 (MQ=255)

                                                                            cTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGcc              <  1:5578389/93‑1 (MQ=255)

                                                                              ggATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAg            >  1:1383464/1‑93 (MQ=255)

                                                                               gATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAgg           <  1:3071868/93‑2 (MQ=255)

                                                                               gATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGc           <  1:1243857/93‑1 (MQ=255)

                                                                               gATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGc           <  2:5089094/93‑1 (MQ=255)

                                                                                atatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGcc          <  2:5595799/93‑1 (MQ=255)

                                                                                atatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGcc          <  1:426022/93‑1 (MQ=255)

                                                                                atatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGcc          <  2:3206518/93‑1 (MQ=255)

                                                                                atatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGcc          <  2:4702174/93‑1 (MQ=255)

                                                                                atatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGcc          <  1:343087/93‑1 (MQ=255)

                                                                                 tatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCg         >  2:5138400/1‑93 (MQ=255)

                                                                                 tatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCg         <  2:4503446/93‑1 (MQ=255)

                                                                                 tatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCg         >  1:2189620/1‑93 (MQ=255)

                                                                                 tatGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGCGAAATTTACGGCCAGCCg         >  1:1537980/1‑93 (MQ=255)

                                                                                  atGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGt        >  2:5205617/1‑93 (MQ=255)

                                                                                   tGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGGGAAATTTACGGCCAGCCGTg       >  2:5290074/1‑93 (MQ=255)

                                                                                     cGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCg     <  2:4315441/93‑1 (MQ=255)

                                                                                      gTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCgg    >  1:3784331/1‑93 (MQ=255)

                                                                                       ttACTAGTTGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGa   >  1:364652/1‑93 (MQ=255)

                                                                                       ttACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGa   <  1:2423185/93‑1 (MQ=255)

CAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGAT  >  NC_000913/4133277‑4133457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGATCTG  >  NC_000913/4133275‑4133460

GCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACT                                                                                              >  SRR4302532.2524283/1‑94 (MQ=60)

GCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACT                                                                                              <  SRR4302532.5341500/94‑1 (MQ=60)

GCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACT                                                                                              <  SRR4302532.3539044/94‑1 (MQ=60)

GCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACT                                                                                              >  SRR4302532.1372633/1‑94 (MQ=60)

  CAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCACTGACTTCTTCGTGAACTTGCTGGATATGGGTTACTAG                                                                                            >  SRR4302532.3802356/1‑94 (MQ=60)

  CAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAG                                                                                            >  SRR4302532.5819441/1‑94 (MQ=60)

   AACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGT                                                                                           >  SRR4302532.4431811/1‑94 (MQ=60)

    ACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTG                                                                                          >  SRR4302532.2580660/1‑94 (MQ=60)

    ACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTG                                                                                          >  SRR4302532.594585/1‑94 (MQ=60)

     CTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGG                                                                                         >  SRR4302532.3332804/1‑94 (MQ=60)

     CTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGG                                                                                         >  SRR4302532.4111625/1‑94 (MQ=60)

        CGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAA                                                                                      <  SRR4302532.520459/94‑1 (MQ=60)

          ATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGC                                                                                    >  SRR4302532.3803163/1‑94 (MQ=60)

          ATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGC                                                                                    >  SRR4302532.2049932/1‑94 (MQ=60)

          ATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGC                                                                                    <  SRR4302532.1528168/94‑1 (MQ=60)

            GGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGA                                                                                  <  SRR4302532.600330/94‑1 (MQ=60)

             GCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGAC                                                                                 >  SRR4302532.3744617/1‑94 (MQ=60)

              CAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACC                                                                                <  SRR4302532.3587383/94‑1 (MQ=60)

              CAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACC                                                                                <  SRR4302532.5890733/94‑1 (MQ=60)

              CAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACC                                                                                >  SRR4302532.3363653/1‑94 (MQ=60)

              CAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACC                                                                                <  SRR4302532.4710063/94‑1 (MQ=60)

              CAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACC                                                                                >  SRR4302532.1872501/1‑94 (MQ=60)

               AGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCG                                                                               >  SRR4302532.734450/1‑94 (MQ=60)

               AGCAAAAACGACGGCTTCACTGACCGCCTTGGTCTATTGAGCAATTACCTCTTCGTGTACTTGCTGGATATGCGTTACTAGTGGAGAGCGACCG                                                                               >  SRR4302532.4327257/1‑94 (MQ=42)

                 CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGAC                                                                             <  SRR4302532.4862980/94‑1 (MQ=60)

                 CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGAC                                                                             <  SRR4302532.2130726/94‑1 (MQ=60)

                 CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGAC                                                                             <  SRR4302532.4130217/94‑1 (MQ=60)

                 CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGAC                                                                             >  SRR4302532.3901526/1‑94 (MQ=60)

                 CAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGAC                                                                             <  SRR4302532.4950867/94‑1 (MQ=60)

                  AAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACG                                                                            >  SRR4302532.4599650/1‑94 (MQ=60)

                  AAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACG                                                                            >  SRR4302532.658412/1‑94 (MQ=60)

                  AAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACG                                                                            >  SRR4302532.4948050/1‑94 (MQ=60)

                      ACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATC                                                                        >  SRR4302532.4533722/1‑94 (MQ=60)

                        GGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGA                                                                      >  SRR4302532.5247153/1‑94 (MQ=60)

                        GGCGTCTTCACTGACCGCGTTGGCGGATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGA                                                                      >  SRR4302532.1324575/1‑94 (MQ=60)

                          CGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGCTACTAACGGAAAGCGACAGACGCATCGAAA                                                                    >  SRR4302532.1639855/1‑94 (MQ=60)

                           GTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAG                                                                   <  SRR4302532.1554485/94‑1 (MQ=60)

                           GTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAG                                                                   <  SRR4302532.5362431/94‑1 (MQ=60)

                           GTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAG                                                                   <  SRR4302532.6307353/94‑1 (MQ=60)

                           GTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAG                                                                   >  SRR4302532.3330591/1‑94 (MQ=60)

                             CTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAG                                                                 <  SRR4302532.5607527/94‑1 (MQ=60)

                             CTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAG                                                                 >  SRR4302532.1052941/1‑94 (MQ=60)

                             CTTCACTGACCGCGTTGGCGTATGGAGCAATGACTGCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAG                                                                 <  SRR4302532.312280/94‑1 (MQ=60)

                               TCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCT                                                               <  SRR4302532.4591586/94‑1 (MQ=60)

                               TCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCT                                                               >  SRR4302532.5313953/1‑94 (MQ=60)

                                CACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTG                                                              >  SRR4302532.2534857/1‑94 (MQ=60)

                                CACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTG                                                              >  SRR4302532.4490599/1‑94 (MQ=60)

                                  CTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTT                                                            <  SRR4302532.193498/94‑1 (MQ=60)

                                  CTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTT                                                            <  SRR4302532.6277702/94‑1 (MQ=60)

                                  CTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTT                                                            <  SRR4302532.2532129/94‑1 (MQ=60)

                                   TGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTC                                                           >  SRR4302532.5404911/1‑94 (MQ=60)

                                   TGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTC                                                           <  SRR4302532.2944124/94‑1 (MQ=60)

                                   TGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTC                                                           >  SRR4302532.2188044/1‑94 (MQ=60)

                                    GACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCG                                                          <  SRR4302532.5601769/94‑1 (MQ=60)

                                    GACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCG                                                          >  SRR4302532.3921448/1‑94 (MQ=60)

                                    GACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCA                                                          >  SRR4302532.5538912/1‑94 (MQ=60)

                                    GACCGCGTTGGCGGATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCG                                                          <  SRR4302532.2366856/94‑1 (MQ=60)

                                     ACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGA                                                         <  SRR4302532.3017617/94‑1 (MQ=60)

                                     ACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGA                                                         >  SRR4302532.513453/1‑94 (MQ=60)

                                      CCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAA                                                        >  SRR4302532.1841534/1‑94 (MQ=60)

                                       CGCGTTGTCGTATTGATCAATGTCGTCTTCGTGAACTTGCTGGATATGCGTTACTAGGGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAG                                                       <  SRR4302532.3940127/94‑1 (MQ=60)

                                       CGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAG                                                       >  SRR4302532.1622529/1‑94 (MQ=60)

                                       CGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAG                                                       >  SRR4302532.2495078/1‑94 (MQ=60)

                                       CGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAG                                                       >  SRR4302532.1253075/1‑94 (MQ=60)

                                       CGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAG                                                       <  SRR4302532.1709182/94‑1 (MQ=60)

                                        GCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGG                                                      <  SRR4302532.4112906/94‑1 (MQ=60)

                                         CGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGC                                                     >  SRR4302532.2583692/1‑94 (MQ=60)

                                         CGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGC                                                     >  SRR4302532.2583693/1‑94 (MQ=60)

                                          GTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCC                                                    <  SRR4302532.2545365/94‑1 (MQ=60)

                                          GTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCC                                                    >  SRR4302532.2905317/1‑94 (MQ=60)

                                            TGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGT                                                  <  SRR4302532.4136083/94‑1 (MQ=60)

                                              GCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGA                                                <  SRR4302532.6012933/94‑1 (MQ=60)

                                               CGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGAC                                               <  SRR4302532.5097173/94‑1 (MQ=60)

                                                GTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACC                                              <  SRR4302532.577920/94‑1 (MQ=60)

                                                 TATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCG                                             >  SRR4302532.2557794/1‑94 (MQ=60)

                                                 TATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCG                                             >  SRR4302532.4364485/1‑94 (MQ=60)

                                                  ATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGG                                            >  SRR4302532.5653453/1‑94 (MQ=60)

                                                   TTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTG                                           <  SRR4302532.593227/94‑1 (MQ=60)

                                                   TTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTG                                           <  SRR4302532.2016191/94‑1 (MQ=60)

                                                   TTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTG                                           >  SRR4302532.1036574/1‑94 (MQ=60)

                                                      AGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAA                                        <  SRR4302532.1454752/94‑1 (MQ=60)

                                                       GCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAAC                                       <  SRR4302532.3299610/94‑1 (MQ=60)

                                                        CAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACG                                      >  SRR4302532.6290473/1‑94 (MQ=60)

                                                        CAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACC                                      >  SRR4302532.6290472/1‑94 (MQ=60)

                                                         AATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCG                                     >  SRR4302532.6338218/1‑94 (MQ=60)

                                                         AATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCG                                     >  SRR4302532.1060133/1‑94 (MQ=60)

                                                         AATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCG                                     >  SRR4302532.5949463/1‑94 (MQ=60)

                                                         AATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGAACGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCG                                     >  SRR4302532.3306824/1‑94 (MQ=60)

                                                          ATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCTTGAAACCGG                                    <  SRR4302532.2701801/94‑1 (MQ=60)

                                                          ATGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGG                                    >  SRR4302532.4471749/1‑94 (MQ=60)

                                                          ATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGG                                    >  SRR4302532.5430084/1‑94 (MQ=60)

                                                           TGACTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGC                                   <  SRR4302532.2589438/94‑1 (MQ=60)

                                                              CTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAA                                >  SRR4302532.4240817/1‑94 (MQ=60)

                                                              CTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAA                                >  SRR4302532.4175793/1‑94 (MQ=60)

                                                              CTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAA                                >  SRR4302532.2937200/1‑94 (MQ=60)

                                                              CTTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAA                                >  SRR4302532.3700168/1‑94 (MQ=60)

                                                               TTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAG                               <  SRR4302532.3554251/94‑1 (MQ=60)

                                                               TTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAG                               >  SRR4302532.5871146/1‑94 (MQ=60)

                                                               TTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAG                               >  SRR4302532.483652/1‑94 (MQ=60)

                                                               TTCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAG                               >  SRR4302532.3822479/1‑94 (MQ=60)

                                                                TCTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGG                              >  SRR4302532.3146292/1‑94 (MQ=60)

                                                                 CTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTG                             <  SRR4302532.2651185/94‑1 (MQ=60)

                                                                 CTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTG                             <  SRR4302532.4062717/94‑1 (MQ=60)

                                                                 CTTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGAGACCGACGAATCGAAAGAGCTGGTCGAAGGCCGTGACCGTGAAACCGGCGAAGTG                             >  SRR4302532.6121514/1‑94 (MQ=60)

                                                                  TTCGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGA                            >  SRR4302532.937775/1‑94 (MQ=60)

                                                                    CGTGAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAA                          >  SRR4302532.851673/1‑94 (MQ=60)

                                                                       GAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTT                       <  SRR4302532.217895/94‑1 (MQ=60)

                                                                       GAACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTT                       >  SRR4302532.6081443/1‑94 (MQ=60)

                                                                        AACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTA                      >  SRR4302532.6206950/1‑94 (MQ=60)

                                                                         ACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTAC                     <  SRR4302532.2630402/94‑1 (MQ=60)

                                                                         ACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTAC                     <  SRR4302532.4682428/94‑1 (MQ=60)

                                                                         ACTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTAC                     <  SRR4302532.1167387/94‑1 (MQ=60)

                                                                          CTTGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGGGAAATTTACG                    >  SRR4302532.1133722/1‑94 (MQ=60)

                                                                            TGGTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGAGAAATTTACGGC                  >  SRR4302532.4504099/1‑94 (MQ=60)

                                                                            TGCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGC                  <  SRR4302532.3186681/94‑1 (MQ=60)

                                                                             GCTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCC                 <  SRR4302532.677394/94‑1 (MQ=60)

                                                                             GCGGGATATGCGTTACGAGTGGAGAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACAGGCGAAGTGAAATTTACGGCC                 <  SRR4302532.4361134/94‑1 (MQ=60)

                                                                              CTGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCA                <  SRR4302532.5772068/94‑1 (MQ=60)

                                                                               TGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAG               >  SRR4302532.1429823/1‑94 (MQ=60)

                                                                               TGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAG               >  SRR4302532.3289174/1‑94 (MQ=60)

                                                                               TGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAG               >  SRR4302532.3925178/1‑94 (MQ=60)

                                                                               TGGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAG               >  SRR4302532.1429822/1‑94 (MQ=60)

                                                                                GGATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGC              >  SRR4302532.1638533/1‑94 (MQ=60)

                                                                                 GATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGGC             <  SRR4302532.3173359/94‑1 (MQ=60)

                                                                                 GATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCC             >  SRR4302532.4699897/1‑94 (MQ=60)

                                                                                 GATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCC             <  SRR4302532.1285474/94‑1 (MQ=60)

                                                                                  ATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCG            <  SRR4302532.354991/94‑1 (MQ=60)

                                                                                  ATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCG            <  SRR4302532.440776/94‑1 (MQ=60)

                                                                                  ATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCG            <  SRR4302532.584771/94‑1 (MQ=60)

                                                                                  ATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCG            >  SRR4302532.2262617/1‑94 (MQ=60)

                                                                                  ATATGCGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGCGAAATTTACGGCCAGCCG            >  SRR4302532.1589946/1‑94 (MQ=60)

                                                                                    ATGCGTTACTGGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTG          <  SRR4302532.1377052/94‑1 (MQ=60)

                                                                                       CGTTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGG       >  SRR4302532.3911957/1‑94 (MQ=60)

                                                                                        GTTACTAGTTGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGA      >  SRR4302532.377290/1‑94 (MQ=60)

                                                                                         TTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGAT     <  SRR4302532.2503718/94‑1 (MQ=60)

                                                                                         TTACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGAT     >  SRR4302532.1272272/1‑94 (MQ=60)

                                                                                           ACTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGATCT   >  SRR4302532.4780770/1‑94 (MQ=60)

                                                                                            CTAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGCGCGGATCTG  >  SRR4302532.4357886/1‑94 (MQ=60)

GCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGACCGTGAAACCGGCGAAGTGAAATTTACGGCCAGCCGTGCGGATCTG  >  NC_000913/4133275‑4133460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`