Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,593,336 +G intergenic (‑23/‑208) yjjM ← / → yjjN hypothetical protein/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,3351.G99.6% 803.0 / ‑4.0 221intergenic (‑22/‑209)yjjM/yjjNhypothetical protein/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (106/114);  total (106/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 250 total aligned reads displayed.

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATA  >  NC_000913/4593243‑4593421
                                                                                             |                                                                                      
tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAt                                                                                         >  2:3903972/1‑93 (MQ=255)
tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAt                                                                                         >  2:4197915/1‑93 (MQ=255)
tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAt                                                                                         >  1:1731094/1‑93 (MQ=255)
tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACCTTGCGAt                                                                                         <  1:6030660/93‑1 (MQ=255)
 gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑g                                                                                       <  1:4593524/93‑1 (MQ=255)
 gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑g                                                                                       <  2:5805215/93‑1 (MQ=255)
  cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gg                                                                                      <  2:409825/93‑2 (MQ=255)
  cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gg                                                                                      <  2:4748732/93‑2 (MQ=255)
    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaa                                                                                    <  2:5206620/93‑4 (MQ=255)
    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaa                                                                                    >  1:2460736/1‑90 (MQ=255)
     cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaag                                                                                   <  1:2182626/93‑5 (MQ=255)
          cgccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTgggg                                                                               <  2:2183123/90‑1 (MQ=255)
           gccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGa                                                                              >  2:320937/3‑93 (MQ=255)
             cgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAg                                                                            <  2:1460695/93‑1 (MQ=255)
             cgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGACAGCGCACTTTGCGATGGAAGTGGGGAAg                                                                            <  2:4320710/93‑1 (MQ=255)
              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAgg                                                                           <  2:618823/93‑1 (MQ=255)
                aTTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGa                                                                         <  1:3178526/93‑1 (MQ=255)
                aTTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGa                                                                         >  1:1498433/1‑93 (MQ=255)
                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGat                                                                        >  2:6093846/1‑93 (MQ=255)
                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGat                                                                        >  2:3764093/1‑93 (MQ=255)
                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGata                                                                       >  2:1472274/1‑93 (MQ=255)
                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGata                                                                       >  2:5668660/1‑93 (MQ=255)
                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGata                                                                       <  1:1714784/93‑1 (MQ=255)
                   cgcgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGatat                                                                      <  1:1341297/93‑1 (MQ=255)
                     cgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGa                                                                    <  2:752472/93‑1 (MQ=255)
                     cgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGa                                                                    >  2:2326622/1‑93 (MQ=255)
                     cgTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGa                                                                    >  2:4952789/1‑93 (MQ=255)
                       tAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAcc                                                                  >  2:5908297/1‑93 (MQ=255)
                             gCTGAGAATATGACGCACTGTGGAGCGGCTGATGTTGTACATTTCTGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCgcgc                                                            >  2:2665537/1‑93 (MQ=255)
                              cTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGcc                                                           <  1:5578768/93‑1 (MQ=255)
                               tGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCa                                                          <  2:4417895/93‑1 (MQ=255)
                                gagaATATGACGCGCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                         >  2:3942051/1‑93 (MQ=255)
                                gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                         >  1:3213272/1‑93 (MQ=255)
                                gagaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCat                                                         <  2:681054/93‑1 (MQ=255)
                                 agaATATGACGCACTGTGTTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                        <  2:1886642/93‑1 (MQ=255)
                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                        <  1:1235504/93‑1 (MQ=255)
                                 agaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCata                                                        <  1:3094363/93‑1 (MQ=255)
                                  gaATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTGTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCatat                                                       <  2:2578349/93‑1 (MQ=255)
                                   aaTATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatc                                                      <  2:1265727/93‑1 (MQ=255)
                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                     <  1:2410600/93‑1 (MQ=255)
                                    atatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatca                                                     >  1:49529/1‑93 (MQ=255)
                                         aCGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAt                                                <  2:5459751/93‑1 (MQ=255)
                                          cGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATg                                               <  2:4017805/93‑1 (MQ=255)
                                          cGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATg                                               <  2:3511340/93‑1 (MQ=255)
                                           gctcTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                              <  2:1710722/90‑1 (MQ=255)
                                           gCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGa                                              >  2:4371773/1‑93 (MQ=255)
                                            gaCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                             <  2:1838921/92‑1 (MQ=255)
                                            cACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                             >  2:3207604/1‑93 (MQ=255)
                                            cACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                             <  1:5734505/93‑1 (MQ=255)
                                            cACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                             <  1:5191547/93‑1 (MQ=255)
                                            cACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                             >  2:1315428/1‑93 (MQ=255)
                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                            >  2:5093279/1‑93 (MQ=255)
                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                            <  1:2107768/93‑1 (MQ=255)
                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                            <  1:2313711/93‑1 (MQ=255)
                                              cTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCt                                           >  2:2873840/1‑93 (MQ=255)
                                               tgtgGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATTACCTg                                          <  1:3290103/93‑1 (MQ=255)
                                               tgtgGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTg                                          <  1:6103567/93‑1 (MQ=255)
                                                 tggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGt                                        >  2:1591549/1‑93 (MQ=255)
                                                  ggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGtt                                       <  1:5858279/93‑1 (MQ=255)
                                                  ggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGtt                                       <  1:73936/93‑1 (MQ=255)
                                                    tgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTaa                                     <  1:400556/93‑1 (MQ=255)
                                                     gCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAt                                    >  2:2110973/1‑93 (MQ=255)
                                                      cGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATc                                   <  1:2812051/93‑1 (MQ=255)
                                                       ggCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATca                                  >  1:3713284/1‑93 (MQ=255)
                                                        gCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcac                                 >  1:4088496/1‑93 (MQ=255)
                                                        gCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcac                                 >  2:2982912/1‑93 (MQ=255)
                                                         cTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcaca                                <  1:4456458/93‑1 (MQ=255)
                                                         cTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcaca                                <  2:2836904/93‑1 (MQ=255)
                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                               <  2:3636127/93‑1 (MQ=255)
                                                           gATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                              >  2:5265038/1‑93 (MQ=255)
                                                           gATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                              >  1:1281462/1‑93 (MQ=255)
                                                           gATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                              >  2:4687718/1‑93 (MQ=255)
                                                           gATGTTGTACATTTCCGCCAGCGAACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                              <  1:3524794/93‑1 (MQ=255)
                                                            aTGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtg                             <  1:2019132/93‑1 (MQ=255)
                                                             tgttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCAGCGCCATATCATCAATGACCTGGTTAATCACATtgt                            <  1:1856380/93‑1 (MQ=255)
                                                                tgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGc                         >  2:5725928/1‑93 (MQ=255)
                                                                tgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGc                         >  2:6028885/1‑93 (MQ=255)
                                                                tgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGc                         >  1:3618878/1‑93 (MQ=255)
                                                                  tACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGt                       <  2:5360574/93‑1 (MQ=255)
                                                                     aTTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTaaa                    <  2:2980622/93‑1 (MQ=255)
                                                                     aTTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTaaa                    <  1:5983245/93‑1 (MQ=255)
                                                                       ttCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAtt                  <  1:3829868/93‑1 (MQ=255)
                                                                       ttCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAtt                  >  1:5771469/1‑93 (MQ=255)
                                                                        tCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAttt                 <  1:2611365/93‑1 (MQ=255)
                                                                        tCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAttt                 <  1:4560483/93‑1 (MQ=255)
                                                                        tCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGCGGCGTAAAttt                 >  2:2629669/1‑93 (MQ=255)
                                                                         ccGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGCCCTGGTTAATCACATTGTGGCGTAAAtttt                <  1:524326/93‑1 (MQ=255)
                                                                             cAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGa            >  1:4363648/1‑93 (MQ=255)
                                                                              aGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGaa           <  1:1013158/93‑1 (MQ=255)
                                                                              aGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGAGGCGTAAATTTTGTGaa           <  1:5471911/93‑1 (MQ=255)
                                                                                 gcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa        <  1:4758329/93‑1 (MQ=255)
                                                                                 gcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa        <  2:1086111/93‑1 (MQ=255)
                                                                                 gcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa        >  2:3310736/1‑93 (MQ=255)
                                                                                  cACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAc       <  1:4868251/93‑1 (MQ=255)
                                                                                   aCTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt      <  2:826196/93‑1 (MQ=255)
                                                                                   aCTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt      <  1:2364727/93‑1 (MQ=255)
                                                                                   aCTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt      >  2:3488278/1‑93 (MQ=255)
                                                                                     tttGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCa    >  1:4059900/1‑93 (MQ=255)
                                                                                     tttGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCa    <  1:5023563/93‑1 (MQ=255)
                                                                                      ttGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGCCTCat   <  2:3289512/93‑1 (MQ=255)
                                                                                             |                                                                                      
TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATA  >  NC_000913/4593243‑4593421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  NC_000913/4593243‑4593429
                                                                                              |                                                                                              
tttttttagacgtCGCATGCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGTGAT‑G                                                                                                >  SRR4302532.5680144/14‑94 (MQ=60)
TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACCTTGCGAT‑G                                                                                                <  SRR4302532.6238935/94‑1 (MQ=60)
 GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑G                                                                                              <  SRR4302532.4750114/94‑1 (MQ=60)
   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                                                                                             >  SRR4302532.2765597/1‑91 (MQ=60)
   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                                                                                             >  SRR4302532.6319999/1‑91 (MQ=60)
   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                                                                                             >  SRR4302532.2542609/1‑91 (MQ=60)
    TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAG                                                                                            >  SRR4302532.2613846/1‑94 (MQ=60)
    TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GTAAG                                                                                            <  SRR4302532.6216738/94‑1 (MQ=60)
     taatacggcgccTTCGCGTAAGGGGCTGAGAATATGACGCCCTGTGGTGGCGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT                                                                                           <  SRR4302532.1247981/82‑1 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCCCTGTGGTGCGGCTGATGTTGTACATTTTCGCCAGCGCACTTTTCGAT‑Gggagt                                                                                           >  SRR4302532.6207966/1‑89 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT                                                                                           <  SRR4302532.2255455/94‑1 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT                                                                                           <  SRR4302532.3085300/94‑1 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaagg                                                                                           >  SRR4302532.4156541/1‑89 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaagg                                                                                           >  SRR4302532.5143720/1‑89 (MQ=60)
     CAAGACGCCGCATTCGCGTAAGTAGCTGAGAATATGACGCACTGTGGTGCGGCGGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT                                                                                           <  SRR4302532.4394194/94‑1 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG                                                                                          >  SRR4302532.28163/1‑94 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG                                                                                          >  SRR4302532.5701417/1‑94 (MQ=60)
      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG                                                                                          >  SRR4302532.5769985/1‑94 (MQ=60)
       AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGG                                                                                         >  SRR4302532.3003306/1‑94 (MQ=60)
        gacgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGG                                                                                        <  SRR4302532.1129840/89‑1 (MQ=60)
             CGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGG                                                                                   >  SRR4302532.1846899/1‑94 (MQ=60)
             CGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGG                                                                                   >  SRR4302532.5966548/1‑94 (MQ=60)
              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGG                                                                                  <  SRR4302532.5504267/94‑1 (MQ=60)
               CATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGA                                                                                 >  SRR4302532.1548979/1‑94 (MQ=60)
                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGAT                                                                                <  SRR4302532.3284081/94‑1 (MQ=60)
                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGAT                                                                                <  SRR4302532.2263006/94‑1 (MQ=60)
                 TTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATA                                                                               <  SRR4302532.3234101/94‑1 (MQ=60)
                 TTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATA                                                                               <  SRR4302532.5525785/94‑1 (MQ=60)
                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATAT                                                                              <  SRR4302532.1772587/94‑1 (MQ=60)
                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATAT                                                                              <  SRR4302532.3858759/94‑1 (MQ=60)
                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATAT                                                                              <  SRR4302532.4832269/94‑1 (MQ=60)
                   CGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATG                                                                             <  SRR4302532.1386153/94‑1 (MQ=60)
                    GCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGA                                                                            <  SRR4302532.2000923/94‑1 (MQ=60)
                    GCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGA                                                                            >  SRR4302532.1404579/1‑94 (MQ=60)
                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACC                                                                          >  SRR4302532.6233580/1‑94 (MQ=60)
                       TAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCC                                                                         >  SRR4302532.2411148/1‑94 (MQ=60)
                       TAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCC                                                                         >  SRR4302532.2525743/1‑94 (MQ=60)
                       TAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCC                                                                         >  SRR4302532.4117069/1‑94 (MQ=60)
                        AAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCC                                                                        <  SRR4302532.4270895/94‑1 (MQ=60)
                          GTGGCTGAGAATATGACGCGCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGC                                                                      <  SRR4302532.5173501/94‑1 (MQ=60)
                          GTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGC                                                                      <  SRR4302532.6179669/94‑1 (MQ=60)
                           TGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCG                                                                     <  SRR4302532.1787763/94‑1 (MQ=60)
                             GCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCC                                                                   >  SRR4302532.1258509/1‑94 (MQ=60)
                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCA                                                                  <  SRR4302532.5772462/94‑1 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 >  SRR4302532.3643638/1‑94 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 >  SRR4302532.3319967/1‑94 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 >  SRR4302532.1931911/1‑94 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 >  SRR4302532.5805854/1‑94 (MQ=60)
                               TGAGAATATGACGCACTGTGGTGCGGCTAATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCAT                                                                 <  SRR4302532.2206862/94‑1 (MQ=60)
                                 AGAATATGACGCGCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302532.1276829/94‑1 (MQ=60)
                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302532.3196944/94‑1 (MQ=60)
                                 AGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATAT                                                               <  SRR4302532.1276828/94‑1 (MQ=60)
                                  GAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATC                                                              <  SRR4302532.4668584/94‑1 (MQ=60)
                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCA                                                             >  SRR4302532.51287/1‑94 (MQ=60)
                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                            <  SRR4302532.2490792/94‑1 (MQ=60)
                                     TATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATC                                                           >  SRR4302532.2144090/1‑94 (MQ=60)
                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                          <  SRR4302532.788873/94‑1 (MQ=60)
                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                        <  SRR4302532.5734437/94‑1 (MQ=60)
                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                        <  SRR4302532.2251215/94‑1 (MQ=60)
                                           GCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACCTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCCCGCCATATCATCAATGAC                                                     >  SRR4302532.3304252/1‑94 (MQ=60)
                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    >  SRR4302532.2763496/1‑94 (MQ=60)
                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    <  SRR4302532.5370474/94‑1 (MQ=60)
                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    <  SRR4302532.5934183/94‑1 (MQ=60)
                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                    >  SRR4302532.1292087/1‑94 (MQ=60)
                                             ACTGTGGTGCGTCTGATTTTGTACATCTCCGCCAGCGCACTTTGCGATGG‑AAGTGGCGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   <  SRR4302532.3620780/94‑1 (MQ=60)
                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   <  SRR4302532.2178117/94‑1 (MQ=60)
                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                   <  SRR4302532.2390719/94‑1 (MQ=60)
                                              CTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTG                                                  >  SRR4302532.5213982/1‑94 (MQ=60)
                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATTACCTGG                                                 <  SRR4302532.3399557/94‑1 (MQ=60)
                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                 <  SRR4302532.6314199/94‑1 (MQ=60)
                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                 <  SRR4302532.290816/94‑1 (MQ=60)
                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                                >  SRR4302532.4603158/1‑94 (MQ=60)
                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                                >  SRR4302532.135194/1‑94 (MQ=60)
                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                                <  SRR4302532.1443416/94‑1 (MQ=60)
                                                 TGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTT                                               >  SRR4302532.2523938/1‑94 (MQ=60)
                                                  GGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTA                                              <  SRR4302532.6061408/94‑1 (MQ=60)
                                                  GGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTA                                              <  SRR4302532.76584/94‑1 (MQ=60)
                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                            <  SRR4302532.414541/94‑1 (MQ=60)
                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          <  SRR4302532.2906194/94‑1 (MQ=60)
                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          <  SRR4302532.5090275/94‑1 (MQ=60)
                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                          >  SRR4302532.3837921/1‑94 (MQ=60)
                                                       GGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCAC                                         >  SRR4302532.4225730/1‑94 (MQ=60)
                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                        >  SRR4302532.5789835/1‑94 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       <  SRR4302532.4608355/94‑1 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       >  SRR4302532.3214868/1‑94 (MQ=60)
                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                       <  SRR4302532.6300640/94‑1 (MQ=60)
                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                      <  SRR4302532.422944/94‑1 (MQ=60)
                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                      >  SRR4302532.4762132/1‑94 (MQ=60)
                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                      >  SRR4302532.1324327/1‑94 (MQ=60)
                                                           GATGTTGTACATTTCCGCCAGCGAACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTG                                     <  SRR4302532.3642765/94‑1 (MQ=60)
                                                            ATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGT                                    <  SRR4302532.2086443/94‑1 (MQ=60)
                                                            ATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGT                                    >  SRR4302532.4157644/1‑94 (MQ=60)
                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCAGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                   <  SRR4302532.1917930/94‑1 (MQ=60)
                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  >  SRR4302532.375902/1‑94 (MQ=60)
                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                  >  SRR4302532.2420324/1‑94 (MQ=60)
                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 >  SRR4302532.3244060/1‑94 (MQ=60)
                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                 >  SRR4302532.3739998/1‑94 (MQ=60)
                                                                TGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                                >  SRR4302532.5997888/1‑94 (MQ=60)
                                                                     ATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAT                           <  SRR4302532.1782155/94‑1 (MQ=60)
                                                                     ATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAT                           <  SRR4302532.1749526/94‑1 (MQ=60)
                                                                     ATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAT                           <  SRR4302532.6189973/94‑1 (MQ=60)
                                                                      TTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATT                          >  SRR4302532.5972297/1‑94 (MQ=60)
                                                                       TTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTT                         >  SRR4302532.5072167/1‑94 (MQ=60)
                                                                       TTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTT                         <  SRR4302532.3959163/94‑1 (MQ=60)
                                                                       TTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTT                         <  SRR4302532.2590524/94‑1 (MQ=60)
                                                                        TCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        <  SRR4302532.5404527/94‑1 (MQ=60)
                                                                        TCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        <  SRR4302532.4716052/94‑1 (MQ=60)
                                                                        TCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTT                        <  SRR4302532.2698723/94‑1 (MQ=60)
                                                                         CCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGCCCTGGTTAATCACATTGTGGCGTAAATTTTG                       <  SRR4302532.542628/94‑1 (MQ=60)
                                                                          CGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGT                      <  SRR4302532.248665/94‑1 (MQ=60)
                                                                           GCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTG                     >  SRR4302532.6051626/1‑94 (MQ=60)
                                                                            CCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGA                    >  SRR4302532.4633758/1‑94 (MQ=60)
                                                                            CCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGA                    >  SRR4302532.4511599/1‑94 (MQ=60)
                                                                             CAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAA                   >  SRR4302532.5017780/1‑94 (MQ=60)
                                                                              AGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAC                  <  SRR4302532.1047573/94‑1 (MQ=60)
                                                                              AGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAC                  <  SRR4302532.333806/94‑1 (MQ=60)
                                                                              AGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAC                  <  SRR4302532.333807/94‑1 (MQ=60)
                                                                              AGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAC                  >  SRR4302532.3916417/1‑94 (MQ=60)
                                                                              AGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGAGGCGTAAATTTTGTGAAC                  <  SRR4302532.5661620/94‑1 (MQ=60)
                                                                                 GCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAC               <  SRR4302532.4921144/94‑1 (MQ=60)
                                                                                 GCACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAC               <  SRR4302532.5205667/94‑1 (MQ=60)
                                                                                  CACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT              <  SRR4302532.5035646/94‑1 (MQ=60)
                                                                                  CACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT              >  SRR4302532.418701/1‑94 (MQ=60)
                                                                                   ACTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTC             <  SRR4302532.2443610/94‑1 (MQ=60)
                                                                                    CTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            >  SRR4302532.3696221/1‑94 (MQ=60)
                                                                                    CTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            >  SRR4302532.4729247/1‑94 (MQ=60)
                                                                                    CTTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA            >  SRR4302532.4196371/1‑94 (MQ=60)
                                                                                     TTTGCGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCAT           <  SRR4302532.5195862/94‑1 (MQ=60)
                                                                                       TGCGATGG‑AGGTGTGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT         <  SRR4302532.674534/94‑1 (MQ=60)
                                                                                         CGATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT       >  SRR4302532.5816879/1‑94 (MQ=60)
                                                                                          GATGG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTT      >  SRR4302532.1204512/1‑94 (MQ=60)
                                                                                           atgG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTC     >  SRR4302532.4110270/4‑94 (MQ=60)
                                                                                           atgG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTC     >  SRR4302532.3988654/4‑94 (MQ=60)
                                                                                            tgG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCC    >  SRR4302532.1370284/3‑94 (MQ=60)
                                                                                            tgG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCC    <  SRR4302532.1199023/92‑1 (MQ=60)
                                                                                            tgG‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCC    <  SRR4302532.5413099/92‑1 (MQ=60)
                                                                                            G‑G‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   <  SRR4302532.4190435/94‑1 (MQ=60)
                                                                                            G‑G‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT   <  SRR4302532.1839906/94‑1 (MQ=60)
                                                                                              G‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  SRR4302532.6046850/1‑94 (MQ=60)
                                                                                              |                                                                                              
TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑AAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTC  >  NC_000913/4593243‑4593429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑