Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,593,336	+G	intergenic (‑23/‑208)	yjjM ← / → yjjN	hypothetical protein/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,593,335	1	.	G	100.0%	828.1 / NA	224	intergenic (‑22/‑209)	yjjM/yjjN	hypothetical protein/putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (106/118); total (106/118)

Only 100 of 243 total aligned reads displayed.

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT  >  NC_000913/4593243‑4593422

tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAt                                                                                          <  1:6602992/93‑1 (MQ=255)

  cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gg                                                                                       <  1:2106306/93‑2 (MQ=255)

    tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaa                                                                                     <  2:6053852/93‑4 (MQ=255)

     cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaag                                                                                    <  2:1868909/93‑5 (MQ=255)

     cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaag                                                                                    >  1:4751354/1‑89 (MQ=255)

         acgccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTggg                                                                                 <  2:2392386/89‑1 (MQ=255)

           gccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGa                                                                               <  2:5561564/91‑1 (MQ=255)

           gccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGa                                                                               >  2:4725943/3‑93 (MQ=255)

            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGaa                                                                              >  2:909028/2‑93 (MQ=255)

            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGaa                                                                              >  2:5666368/2‑93 (MQ=255)

            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGaa                                                                              <  2:4131351/92‑1 (MQ=255)

            ccgcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGaa                                                                              <  2:371088/92‑1 (MQ=255)

              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGaaag                                                                            >  1:4270401/1‑91 (MQ=255)

              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAgg                                                                            <  1:1203933/93‑1 (MQ=255)

              gcATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAgg                                                                            >  1:1619005/1‑93 (MQ=255)

               cATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAggg                                                                           <  2:2413256/93‑1 (MQ=255)

                aTTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGa                                                                          >  1:3959194/1‑93 (MQ=255)

                 ttCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGat                                                                         >  2:2343422/1‑93 (MQ=255)

                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGata                                                                        <  1:5877869/93‑1 (MQ=255)

                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGata                                                                        <  2:2773542/93‑1 (MQ=255)

                  tCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGata                                                                        <  2:3060336/93‑1 (MQ=255)

                       tAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAcc                                                                   <  1:1268900/93‑1 (MQ=255)

                        aaGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAccc                                                                  >  2:3087271/1‑93 (MQ=255)

                         aGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAcccc                                                                 >  1:779127/1‑93 (MQ=255)

                         aGTGGCTGAGAATATGACGCACTGTGCTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAcccc                                                                 <  1:3116999/93‑1 (MQ=255)

                          gTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCcg                                                                <  2:443057/93‑1 (MQ=255)

                           tcgCTGAGAATATGACGCACTTTGGTGCTGCTGATATTGTTCATTTCCGCCAGCGCACTTGGCGATGGAAGTGGGGAAGGGATATGACCCcgc                                                               <  2:827353/91‑1 (MQ=255)

                           tGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCcgc                                                               <  2:2570389/93‑1 (MQ=255)

                            ggCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCcgcg                                                              >  2:4975748/1‑93 (MQ=255)

                             gCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCgcgc                                                             <  2:2674613/93‑1 (MQ=255)

                             gCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCgcgc                                                             <  1:3013655/93‑1 (MQ=255)

                              cTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGTCCCCGCGcc                                                            <  1:3296322/93‑1 (MQ=255)

                              cTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGcc                                                            <  1:1576982/93‑1 (MQ=255)

                               tGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCa                                                           >  1:2409669/1‑93 (MQ=255)

                                     tatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcat                                                     >  2:1226350/1‑93 (MQ=255)

                                     tatGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcat                                                     >  1:5169202/1‑93 (MQ=255)

                                      atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATatcatc                                                    <  1:5782777/93‑1 (MQ=255)

                                       tGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATAtcatca                                                   <  1:3827434/93‑1 (MQ=255)

                                        gACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCaa                                                  >  2:1279712/1‑93 (MQ=255)

                                         aCGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAt                                                 <  1:3246684/93‑1 (MQ=255)

                                           gCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACACCGCGCCATATCATCAATGa                                               >  1:4417155/1‑93 (MQ=255)

                                            cACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc                                              <  1:6511107/93‑1 (MQ=255)

                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                             <  1:2566179/93‑1 (MQ=255)

                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                             >  1:5636988/1‑93 (MQ=255)

                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                             <  2:2281189/93‑1 (MQ=255)

                                             aCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                             >  2:1455873/1‑93 (MQ=255)

                                             aCTGTGGGGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAcc                                             >  1:6314936/1‑93 (MQ=255)

                                              cTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCt                                            >  1:892423/1‑93 (MQ=255)

                                              cTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCt                                            >  2:506451/1‑93 (MQ=255)

                                               tgtgGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTg                                           >  1:3944199/1‑93 (MQ=255)

                                                gtggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTgg                                          <  2:4170157/93‑1 (MQ=255)

                                                gtggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTgg                                          >  1:199440/1‑93 (MQ=255)

                                                 tggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGt                                         <  2:354806/93‑1 (MQ=255)

                                                 tggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGt                                         >  1:1817884/1‑93 (MQ=255)

                                                  ggtgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGtt                                        <  1:3469611/93‑1 (MQ=255)

                                                  ggtgCGGCGAATGTTGTACATTTCCGCCAGCGCACTTGGCGATGGAAGTGGGGAAAGGATATGACCCCGCGCCATATCATCCATGACCTGGtt                                        <  1:4896333/93‑1 (MQ=255)

                                                    tgCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTaa                                      <  1:3430971/93‑1 (MQ=255)

                                                     gCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAt                                     <  1:4142954/93‑1 (MQ=255)

                                                      cGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATc                                    >  2:3000942/1‑93 (MQ=255)

                                                       ggCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATca                                   <  1:5766382/93‑1 (MQ=255)

                                                        gCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATcac                                  <  1:2544157/93‑1 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGTGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                <  2:3652275/93‑1 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                <  2:399152/93‑1 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                >  1:3586094/1‑93 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                <  1:618307/93‑1 (MQ=255)

                                                          tGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAt                                <  2:525169/93‑1 (MQ=255)

                                                           gATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAtt                               >  1:4620806/1‑93 (MQ=255)

                                                            aTGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtg                              >  2:5052871/1‑93 (MQ=255)

                                                             tgttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGCAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgt                             >  2:4671263/1‑93 (MQ=255)

                                                             tgttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgt                             <  1:2023328/93‑1 (MQ=255)

                                                              gttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATtgtg                            <  2:5723567/93‑1 (MQ=255)

                                                               ttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  1:4271547/93‑1 (MQ=255)

                                                               ttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           >  2:1368426/1‑93 (MQ=255)

                                                               ttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           >  1:697270/1‑93 (MQ=255)

                                                               ttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  2:3568347/93‑1 (MQ=255)

                                                               ttgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTgg                           <  1:5555426/93‑1 (MQ=255)

                                                                tgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGc                          >  2:5676163/1‑93 (MQ=255)

                                                                tgtACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGc                          <  1:3510951/93‑1 (MQ=255)

                                                                  tACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAGCTGGTTAATCACATTGTGGCGt                        >  1:4334127/1‑93 (MQ=255)

                                                                  tACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGt                        >  2:6143937/1‑93 (MQ=255)

                                                                   aCATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTa                       >  1:2171479/1‑93 (MQ=255)

                                                                      tttCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAt                    >  2:3298932/1‑93 (MQ=255)

                                                                         ccGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAAtttt                 <  2:4380347/93‑1 (MQ=255)

                                                                          cGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtg                <  1:4975748/93‑1 (MQ=255)

                                                                          cGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtg                >  1:5452118/1‑93 (MQ=255)

                                                                            ccAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtgtg              >  1:2119454/1‑93 (MQ=255)

                                                                            ccAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtgtg              <  2:6372960/93‑1 (MQ=255)

                                                                            ccAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTtgtg              >  2:5636666/1‑93 (MQ=255)

                                                                             cAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGa             <  2:3765386/93‑1 (MQ=255)

                                                                              aGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGaa            >  2:6598889/1‑93 (MQ=255)

                                                                               gcgcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAc           >  2:2852165/1‑93 (MQ=255)

                                                                                cgcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACg          <  2:199440/93‑1 (MQ=255)

                                                                                 gcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa         >  1:2958782/1‑93 (MQ=255)

                                                                                 gcACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGa         >  1:2849498/1‑93 (MQ=255)

                                                                                  cACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGAc        <  1:1101891/93‑1 (MQ=255)

                                                                                   aCTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACt       <  2:4032484/93‑1 (MQ=255)

                                                                                    cTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTc      <  2:5156114/93‑1 (MQ=255)

                                                                                     tttGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCa     >  2:1800118/1‑93 (MQ=255)

                                                                                     tttGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCa     >  1:3099059/1‑93 (MQ=255)

                                                                                       tGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCata   <  1:522151/93‑1 (MQ=255)

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT  >  NC_000913/4593243‑4593422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT  >  NC_000913/4593243‑4593428

TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G                                                                                              <  SRR4302547.6740437/94‑1 (MQ=60)

TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G                                                                                              <  SRR4302547.3954657/94‑1 (MQ=60)

  CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GGA                                                                                            <  SRR4302547.2148274/94‑1 (MQ=60)

   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                                                                                           <  SRR4302547.2366258/94‑4 (MQ=60)

   GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                                                                                           >  SRR4302547.4949766/1‑91 (MQ=60)

    TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAG                                                                                           >  SRR4302547.4849812/1‑94 (MQ=60)

     CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTTCGCCAGCGCACTTTGCGAT‑Ggaagg                                                                                         >  SRR4302547.4570241/1‑89 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATGTGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                         >  SRR4302547.82274/1‑94 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                         >  SRR4302547.4866077/1‑94 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                         >  SRR4302547.6765958/1‑94 (MQ=60)

      AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG                                                                                         >  SRR4302547.5577563/1‑94 (MQ=60)

        gacgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGG                                                                                       >  SRR4302547.2111881/6‑94 (MQ=60)

          cgcCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGA                                                                                     >  SRR4302547.3455609/4‑94 (MQ=60)

            CCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAG                                                                                   >  SRR4302547.2046136/1‑94 (MQ=60)

             CGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGG                                                                                  >  SRR4302547.763467/1‑94 (MQ=60)

             CGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGG                                                                                  >  SRR4302547.1651048/1‑94 (MQ=60)

             CGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAAG                                                                                  >  SRR4302547.4358046/1‑94 (MQ=60)

              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGG                                                                                 >  SRR4302547.6649412/1‑94 (MQ=60)

              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGG                                                                                 <  SRR4302547.1228233/94‑1 (MQ=60)

              GCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGG                                                                                 >  SRR4302547.528654/1‑94 (MQ=60)

               CATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGA                                                                                >  SRR4302547.3100996/1‑94 (MQ=60)

               CATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGA                                                                                >  SRR4302547.4039728/1‑94 (MQ=60)

                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGAT                                                                               >  SRR4302547.2762562/1‑94 (MQ=60)

                ATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGAT                                                                               >  SRR4302547.5317004/1‑94 (MQ=60)

                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAT                                                                             <  SRR4302547.5999554/94‑1 (MQ=60)

                  TCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAT                                                                             <  SRR4302547.4900299/94‑1 (MQ=60)

                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACC                                                                         >  SRR4302547.3517819/1‑94 (MQ=60)

                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACC                                                                         <  SRR4302547.5214689/94‑1 (MQ=60)

                      GTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACC                                                                         >  SRR4302547.2579125/1‑94 (MQ=60)

                       TAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCC                                                                        <  SRR4302547.1294454/94‑1 (MQ=60)

                        AAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCC                                                                       >  SRR4302547.793854/1‑94 (MQ=60)

                         AGTGGCTGAGAATATGACGCACTGTGCTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCG                                                                      <  SRR4302547.3178782/94‑1 (MQ=60)

                          GTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGC                                                                     >  SRR4302547.3167798/1‑94 (MQ=60)

                             GCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCC                                                                  <  SRR4302547.3073584/94‑1 (MQ=60)

                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGTCCCCGCGCCA                                                                 <  SRR4302547.3362612/94‑1 (MQ=60)

                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCA                                                                 >  SRR4302547.2458453/1‑94 (MQ=60)

                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCA                                                                 <  SRR4302547.1608365/94‑1 (MQ=60)

                              CTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCA                                                                 >  SRR4302547.1948568/1‑94 (MQ=60)

                                GAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATA                                                               <  SRR4302547.1242320/94‑1 (MQ=60)

                                  GAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATC                                                             >  SRR4302547.3338692/1‑94 (MQ=60)

                                   AATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCA                                                            >  SRR4302547.320664/1‑94 (MQ=60)

                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                           <  SRR4302547.2838168/94‑1 (MQ=60)

                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                           >  SRR4302547.1778442/1‑94 (MQ=60)

                                    ATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCAT                                                           >  SRR4302547.5275638/1‑94 (MQ=60)

                                     TATGACGCACTGTGTTGCGGCTGATGTTGTACATTTCCGCCGGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATACCATC                                                          >  SRR4302547.4148021/1‑94 (MQ=60)

                                     TATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATC                                                          <  SRR4302547.563905/94‑1 (MQ=60)

                                     TATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATC                                                          <  SRR4302547.4109529/94‑1 (MQ=60)

                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                         <  SRR4302547.5367102/94‑1 (MQ=60)

                                      ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCA                                                         <  SRR4302547.5902384/94‑1 (MQ=60)

                                       TGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAA                                                        <  SRR4302547.3905394/94‑1 (MQ=60)

                                        GACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAAT                                                       <  SRR4302547.2187683/94‑1 (MQ=60)

                                         ACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATG                                                      <  SRR4302547.3311542/94‑1 (MQ=60)

                                         ACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATG                                                      <  SRR4302547.3689457/94‑1 (MQ=60)

                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCTGCGACATATCATCAATGA                                                     >  SRR4302547.4252678/1‑94 (MQ=60)

                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGA                                                     <  SRR4302547.577537/94‑1 (MQ=60)

                                          CGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACACCGCGCCATATCATCAATGA                                                     >  SRR4302547.4508305/1‑94 (MQ=60)

                                           GCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAC                                                    <  SRR4302547.3621390/94‑1 (MQ=60)

                                            tactattggGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGTGATGGTTGTGGGGAAGGGATATGTCCCCGCGCCACATCAACAATGACC                                                   >  SRR4302547.6793206/10‑94 (MQ=53)

                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                   >  SRR4302547.5753337/1‑94 (MQ=60)

                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                   <  SRR4302547.3308424/94‑1 (MQ=60)

                                            CACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                   <  SRR4302547.6646661/94‑1 (MQ=60)

                                            CACTGTGGGGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACC                                                   >  SRR4302547.6446251/1‑94 (MQ=60)

                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                  >  SRR4302547.909582/1‑94 (MQ=60)

                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCT                                                  <  SRR4302547.2618014/94‑1 (MQ=60)

                                             ACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCG                                                  >  SRR4302547.832628/1‑94 (MQ=60)

                                              CTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTG                                                 >  SRR4302547.4024421/1‑94 (MQ=60)

                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                >  SRR4302547.203159/1‑94 (MQ=60)

                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                >  SRR4302547.2599257/1‑94 (MQ=60)

                                               TGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGG                                                <  SRR4302547.6431417/94‑1 (MQ=60)

                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                               >  SRR4302547.1853811/1‑94 (MQ=60)

                                                GTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGT                                               >  SRR4302547.305578/1‑94 (MQ=60)

                                                 TGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTT                                              <  SRR4302547.4035698/94‑1 (MQ=60)

                                                  GGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTA                                             <  SRR4302547.3540367/94‑1 (MQ=60)

                                                  GGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTA                                             <  SRR4302547.708583/94‑1 (MQ=60)

                                                  GGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTA                                             <  SRR4302547.3158755/94‑1 (MQ=60)

                                                  GGTGCGGCGAATGTTGTACATTTCCGCCAGCGCACTTGGCGATGGAAGTGGGGAAAGGATATGACCCCGCGCCATATCATCCATGACCTGGTTA                                             <  SRR4302547.4997733/94‑1 (MQ=60)

                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                           >  SRR4302547.6767043/1‑94 (MQ=60)

                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                           >  SRR4302547.6097319/1‑94 (MQ=60)

                                                    TGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAAT                                           <  SRR4302547.3500882/94‑1 (MQ=60)

                                                     GCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC                                          <  SRR4302547.4227267/94‑1 (MQ=60)

                                                      CGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCA                                         <  SRR4302547.1644866/94‑1 (MQ=60)

                                                       GGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCAC                                        <  SRR4302547.5885638/94‑1 (MQ=60)

                                                       GGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCAC                                        <  SRR4302547.6103400/94‑1 (MQ=60)

                                                       GGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCAC                                        >  SRR4302547.1784305/1‑94 (MQ=60)

                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                       <  SRR4302547.29414/94‑1 (MQ=60)

                                                        GCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACA                                       <  SRR4302547.2595537/94‑1 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                      >  SRR4302547.4369912/1‑94 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                      >  SRR4302547.3553922/1‑94 (MQ=60)

                                                         CTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACAT                                      >  SRR4302547.3659262/1‑94 (MQ=60)

                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                     >  SRR4302547.4433698/1‑94 (MQ=60)

                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                     >  SRR4302547.4716492/1‑94 (MQ=60)

                                                          TGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATT                                     <  SRR4302547.629757/94‑1 (MQ=60)

                                                            ATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGT                                   >  SRR4302547.5943443/1‑94 (MQ=60)

                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                  <  SRR4302547.98590/94‑1 (MQ=60)

                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                  <  SRR4302547.4762850/94‑1 (MQ=60)

                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                  <  SRR4302547.2063616/94‑1 (MQ=60)

                                                             TGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTG                                  >  SRR4302547.4360886/1‑94 (MQ=60)

                                                              GTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGG                                 >  SRR4302547.710161/1‑94 (MQ=60)

                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                <  SRR4302547.5670021/94‑1 (MQ=60)

                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                <  SRR4302547.5734137/94‑1 (MQ=60)

                                                               TTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGC                                <  SRR4302547.4359222/94‑1 (MQ=60)

                                                                TGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                               <  SRR4302547.3582605/94‑1 (MQ=60)

                                                                TGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                               <  SRR4302547.1175569/94‑1 (MQ=60)

                                                                TGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                               >  SRR4302547.4358742/1‑94 (MQ=60)

                                                                TGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCG                               <  SRR4302547.2054391/94‑1 (MQ=60)

                                                                 GTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAGCTGGTTAATCACATTGTGGCGT                              >  SRR4302547.4423291/1‑94 (MQ=60)

                                                                  TACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTA                             >  SRR4302547.2214943/1‑94 (MQ=60)

                                                                   ACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAA                            <  SRR4302547.6458912/94‑1 (MQ=60)

                                                                      TTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATT                         <  SRR4302547.5068554/94‑1 (MQ=60)

                                                                       TTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTT                        <  SRR4302547.3594766/94‑1 (MQ=60)

                                                                       TTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGAATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTT                        <  SRR4302547.6554998/94‑1 (MQ=60)

                                                                         CCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTG                      <  SRR4302547.3987004/94‑1 (MQ=60)

                                                                         CCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTG                      >  SRR4302547.5564785/1‑94 (MQ=60)

                                                                          CGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGT                     <  SRR4302547.5078674/94‑1 (MQ=60)

                                                                           GCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTG                    >  SRR4302547.2161700/1‑94 (MQ=60)

                                                                            CCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGA                   >  SRR4302547.4606717/1‑94 (MQ=60)

                                                                             CAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAC                  >  SRR4302547.4566719/1‑94 (MQ=60)

                                                                              AGCTCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAAC                 <  SRR4302547.1602047/94‑1 (MQ=60)

                                                                               GCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACG                <  SRR4302547.5894692/94‑1 (MQ=60)

                                                                                CGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGA               >  SRR4302547.3017670/1‑94 (MQ=60)

                                                                                CGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGA               >  SRR4302547.2906427/1‑94 (MQ=60)

                                                                                  CACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT             <  SRR4302547.1124046/94‑1 (MQ=60)

                                                                                  CACTTTGCGATGGAAGCGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACT             >  SRR4302547.2180455/1‑94 (MQ=60)

                                                                                   ACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTC            >  SRR4302547.5788903/1‑94 (MQ=60)

                                                                                   ACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTC            >  SRR4302547.1519820/1‑94 (MQ=60)

                                                                                   ACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTC            >  SRR4302547.5613711/1‑94 (MQ=60)

                                                                                    CTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCA           >  SRR4302547.3160453/1‑94 (MQ=60)

                                                                                       TGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT        <  SRR4302547.2154261/94‑1 (MQ=60)

                                                                                       TGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT        <  SRR4302547.349423/94‑1 (MQ=60)

                                                                                       TGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATAT        <  SRR4302547.531610/94‑1 (MQ=60)

                                                                                         CGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT      >  SRR4302547.2151961/1‑94 (MQ=60)

                                                                                         CGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT      >  SRR4302547.3845144/1‑94 (MQ=60)

                                                                                         CGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTT      <  SRR4302547.1853647/94‑1 (MQ=60)

                                                                                         CGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACCCATATTT      <  SRR4302547.5633411/94‑1 (MQ=60)

                                                                                           atgGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTC    >  SRR4302547.6451555/4‑94 (MQ=60)

                                                                                            G‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT  >  SRR4302547.2485008/1‑94 (MQ=60)

TGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCT  >  NC_000913/4593243‑4593428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`