Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,078	C→T	W135* (TGG→TAG)	yjjP ←	putative structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,078	0	C	T	99.6%	940.7 / NA	249	W135* (TGG→TAG)	yjjP	putative structural protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (134/114); minor base A (0/1); total (134/115)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG  >  NC_000913/4599989‑4600167

 aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCt                                                                                       >  2:3599188/1‑93 (MQ=255)

 aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCAATCt                                                                                       <  1:4038037/93‑1 (MQ=255)

  aagaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                      >  1:4004644/1‑93 (MQ=255)

  aagaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                      >  2:4979885/1‑93 (MQ=255)

   agaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTg                                                                                     <  1:2165969/93‑1 (MQ=255)

    gaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTgg                                                                                    >  1:1973111/1‑93 (MQ=255)

     aagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggg                                                                                   >  1:1102174/1‑93 (MQ=255)

     aagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggg                                                                                   <  2:1480008/93‑1 (MQ=255)

     aagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggg                                                                                   >  2:4752647/1‑93 (MQ=255)

      agGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggga                                                                                  >  2:4571598/1‑92 (MQ=255)

        gTGATGACGGCACCATCACAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTaa                                                                                <  1:2334711/93‑1 (MQ=255)

        gTGATGACGGCACCATACCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGTTaa                                                                                <  1:2118076/93‑1 (MQ=255)

         tgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAc                                                                               <  2:4530020/93‑1 (MQ=255)

         tgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAc                                                                               >  1:42467/1‑93 (MQ=255)

           atgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGt                                                                             <  2:1703688/93‑1 (MQ=255)

            tgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTa                                                                            >  2:1435998/1‑93 (MQ=255)

             gaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTaa                                                                           <  2:2035116/93‑1 (MQ=255)

                ggCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATgg                                                                        >  2:1679909/1‑93 (MQ=255)

                 gCACCATCCCAGCCACCGTTATTTAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGc                                                                       <  1:5581974/93‑1 (MQ=255)

                 gCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGc                                                                       >  1:5337941/1‑93 (MQ=255)

                   aCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTg                                                                     <  2:6141143/93‑1 (MQ=255)

                   aCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTg                                                                     >  2:3066363/1‑93 (MQ=255)

                     cATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGaa                                                                   >  1:588763/1‑93 (MQ=255)

                      aTCGCAGCCACCGTTAGTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAt                                                                  <  1:668490/93‑1 (MQ=255)

                       tCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                 >  2:3350150/1‑93 (MQ=255)

                       tCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                 <  1:1195932/93‑1 (MQ=255)

                       tCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                 <  2:2680961/93‑1 (MQ=255)

                        cccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAttt                                                                <  2:3000340/93‑1 (MQ=255)

                        cccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAttt                                                                <  1:6090018/93‑1 (MQ=255)

                         ccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTg                                                               <  2:2967792/93‑1 (MQ=255)

                         ccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTg                                                               <  1:537562/93‑1 (MQ=255)

                              cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaa                                                          >  2:5666088/1‑93 (MQ=255)

                              cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaa                                                          >  2:3139418/1‑93 (MQ=255)

                              cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaa                                                          >  2:221827/1‑93 (MQ=255)

                                ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  1:1412804/93‑1 (MQ=255)

                                ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  1:4466547/93‑1 (MQ=255)

                                ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  2:3291931/93‑1 (MQ=255)

                                ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  1:5045570/93‑1 (MQ=255)

                                ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        >  2:2084250/1‑93 (MQ=255)

                                ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  1:5993709/93‑1 (MQ=255)

                                      ttGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtc                                                  >  2:4146523/1‑93 (MQ=255)

                                       tGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtct                                                 >  2:5119550/1‑93 (MQ=255)

                                       tGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtct                                                 >  2:2101449/1‑93 (MQ=255)

                                        gAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtctc                                                <  1:4183242/93‑1 (MQ=255)

                                        gAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTCAATTTGGCTAAATCGTTtctc                                                >  1:1519421/1‑93 (MQ=255)

                                         aGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTctct                                               <  2:31638/93‑1 (MQ=255)

                                         aGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTctct                                               >  1:3787483/1‑93 (MQ=255)

                                          gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                              <  1:1776419/93‑1 (MQ=255)

                                           tttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAc                                             <  1:258697/93‑1 (MQ=255)

                                            ttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACg                                            <  2:2640360/93‑1 (MQ=255)

                                              aCAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGcc                                          <  1:4331498/93‑1 (MQ=255)

                                               cAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCt                                         >  2:2416856/1‑93 (MQ=255)

                                               cAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCt                                         >  1:97706/1‑93 (MQ=255)

                                                 gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                       >  1:4583642/1‑93 (MQ=255)

                                                 gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                       <  2:153977/93‑1 (MQ=255)

                                                       ggCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATc                                 <  1:5958645/93‑1 (MQ=255)

                                                          gcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcag                              <  2:5317742/93‑1 (MQ=255)

                                                          gcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcag                              >  1:4438590/1‑93 (MQ=255)

                                                           cAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagc                             >  1:3629752/1‑93 (MQ=255)

                                                           cAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagc                             <  1:139804/93‑1 (MQ=255)

                                                            aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            <  1:4740377/93‑1 (MQ=255)

                                                            aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            <  2:2881444/93‑1 (MQ=255)

                                                            aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGACTTTGTAATCcagca                            <  1:1846711/93‑1 (MQ=255)

                                                             aGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                           >  1:3586370/1‑93 (MQ=255)

                                                              gAAAGGCCAACCATTAAGGCTGCCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAga                          >  2:3924706/1‑93 (MQ=255)

                                                              gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAga                          >  1:3285887/1‑93 (MQ=255)

                                                              gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAga                          <  2:5552592/93‑1 (MQ=255)

                                                              gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAga                          <  1:1993088/93‑1 (MQ=255)

                                                              gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAga                          >  1:2397275/1‑93 (MQ=255)

                                                                aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGT‑ATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  1:1594071/93‑1 (MQ=255)

                                                                 aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       >  2:821840/1‑93 (MQ=255)

                                                                 aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  1:5705486/93‑1 (MQ=255)

                                                                 aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       >  1:2703021/1‑93 (MQ=255)

                                                                 aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  1:3150027/93‑1 (MQ=255)

                                                                      aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTc                  >  2:5760577/1‑93 (MQ=255)

                                                                       aCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                 <  2:3941127/93‑1 (MQ=255)

                                                                        ccATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCg                >  2:4598036/1‑93 (MQ=255)

                                                                         cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCga               >  1:5261480/1‑92 (MQ=255)

                                                                         cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc               >  1:1099178/1‑93 (MQ=255)

                                                                          aTTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa              >  2:966190/1‑93 (MQ=255)

                                                                          aTTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa              >  1:5129913/1‑93 (MQ=255)

                                                                           ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             >  2:2809910/1‑93 (MQ=255)

                                                                           ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             >  1:754283/1‑93 (MQ=255)

                                                                           ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             >  2:4305813/1‑93 (MQ=255)

                                                                           ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             >  2:3422172/1‑93 (MQ=255)

                                                                              aGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGaa          >  1:2920692/1‑93 (MQ=255)

                                                                               ggCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAt         >  1:5526876/1‑93 (MQ=255)

                                                                                gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATc        >  1:341942/1‑93 (MQ=255)

                                                                                gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATc        >  1:2462329/1‑93 (MQ=255)

                                                                                gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATc        <  2:6087405/93‑1 (MQ=255)

                                                                                gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATc        <  1:1315336/93‑1 (MQ=255)

                                                                                   aCCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATcaca     >  1:4970432/1‑93 (MQ=255)

                                                                                    ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    <  2:5260078/93‑1 (MQ=255)

                                                                                    ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    >  2:1252469/1‑93 (MQ=255)

                                                                                    ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    >  1:5827462/1‑93 (MQ=255)

                                                                                    ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCATTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    <  2:593518/93‑1 (MQ=255)

                                                                                     cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt   >  1:3925343/1‑93 (MQ=255)

                                                                                     cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt   >  1:4147886/1‑93 (MQ=255)

                                                                                     cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt   <  2:867229/93‑1 (MQ=255)

                                                                                      aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  >  1:6121407/1‑93 (MQ=255)

CAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG  >  NC_000913/4599989‑4600167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  >  NC_000913/4599985‑4600171

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCT                                                                                               >  SRR4302532.1128136/1‑94 (MQ=60)

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCT                                                                                               >  SRR4302532.75144/1‑94 (MQ=60)

  GGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTAT                                                                                             <  SRR4302532.4937904/94‑1 (MQ=60)

  GCCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTAT                                                                                             <  SRR4302532.1854329/94‑1 (MQ=60)

    CAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCT                                                                                           >  SRR4302532.6193034/1‑94 (MQ=60)

     AAAGAAGGTGATGCCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTCAGGCTACCAGCTATCCT                                                                                          >  SRR4302532.6128025/1‑94 (MQ=60)

     AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTT                                                                                          <  SRR4302532.4419807/94‑1 (MQ=60)

     AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTT                                                                                          <  SRR4302532.4596605/94‑1 (MQ=60)

     AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTT                                                                                          >  SRR4302532.4139575/1‑94 (MQ=60)

     AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCAATCTT                                                                                          <  SRR4302532.4174055/94‑1 (MQ=60)

       AGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGG                                                                                        >  SRR4302532.2038650/1‑94 (MQ=60)

       AGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGG                                                                                        <  SRR4302532.2238420/94‑1 (MQ=60)

       AGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGG                                                                                        >  SRR4302532.3859072/1‑94 (MQ=60)

        GAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGG                                                                                       >  SRR4302532.1139027/1‑94 (MQ=60)

        GAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGG                                                                                       <  SRR4302532.2865629/94‑1 (MQ=60)

         AAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGT                                                                                      <  SRR4302532.6208839/94‑1 (MQ=60)

         AAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGT                                                                                      >  SRR4302532.5717032/1‑94 (MQ=60)

           GGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAA                                                                                    >  SRR4302532.2092990/1‑94 (MQ=60)

           GGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAA                                                                                    >  SRR4302532.2092991/1‑94 (MQ=60)

            GTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAC                                                                                   >  SRR4302532.43986/1‑94 (MQ=60)

            GTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAC                                                                                   <  SRR4302532.4629827/94‑1 (MQ=60)

            GTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAC                                                                                   <  SRR4302532.3714122/94‑1 (MQ=60)

            GTGATGACGGCACCATCACAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAC                                                                                   <  SRR4302532.2412480/94‑1 (MQ=60)

            GTGATGACGGCACCATACCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGTTAAC                                                                                   <  SRR4302532.2188847/94‑1 (MQ=60)

             TGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACG                                                                                  <  SRR4302532.5938513/94‑1 (MQ=60)

             TGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACG                                                                                  >  SRR4302532.1605974/1‑94 (MQ=60)

             TGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACG                                                                                  >  SRR4302532.2680120/1‑94 (MQ=60)

             TGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACG                                                                                  >  SRR4302532.2044271/1‑94 (MQ=60)

              GATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGT                                                                                 <  SRR4302532.549392/94‑1 (MQ=60)

                    GGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGC                                                                           >  SRR4302532.5523086/1‑94 (MQ=60)

                     GCACCATCCCAGCCACCGTTATTTAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCT                                                                          <  SRR4302532.5775749/94‑1 (MQ=60)

                     GCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCT                                                                          <  SRR4302532.1407861/94‑1 (MQ=60)

                      CACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTG                                                                         >  SRR4302532.4580621/1‑94 (MQ=60)

                      CACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTG                                                                         <  SRR4302532.5580111/94‑1 (MQ=60)

                       ACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGA                                                                        >  SRR4302532.4738496/1‑94 (MQ=60)

                       ACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGA                                                                        >  SRR4302532.5011608/1‑94 (MQ=60)

                        CCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAA                                                                       >  SRR4302532.609307/1‑94 (MQ=60)

                         CATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAT                                                                      >  SRR4302532.413592/1‑94 (MQ=60)

                          ATCGCAGCCACCGTTAGTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATT                                                                     <  SRR4302532.691552/94‑1 (MQ=60)

                          ATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATT                                                                     >  SRR4302532.428318/1‑94 (MQ=60)

                          ATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATT                                                                     <  SRR4302532.5797692/94‑1 (MQ=60)

                           TCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTT                                                                    <  SRR4302532.1235913/94‑1 (MQ=60)

                            CCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTG                                                                   <  SRR4302532.6300270/94‑1 (MQ=60)

                             CCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGG                                                                  <  SRR4302532.556290/94‑1 (MQ=60)

                             CCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGG                                                                  >  SRR4302532.2756632/1‑94 (MQ=60)

                             CCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGG                                                                  >  SRR4302532.2292966/1‑94 (MQ=60)

                                GCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTA                                                               >  SRR4302532.5526071/1‑94 (MQ=60)

                                GCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTA                                                               >  SRR4302532.4170419/1‑94 (MQ=60)

                                  CACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTCCCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAA                                                             <  SRR4302532.1719725/94‑1 (MQ=60)

                                  CACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAA                                                             >  SRR4302532.5293043/1‑94 (MQ=60)

                                   ACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAT                                                            >  SRR4302532.313364/1‑94 (MQ=60)

                                   ACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAC                                                            >  SRR4302532.5473591/1‑94 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           >  SRR4302532.2512078/1‑94 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           <  SRR4302532.511776/94‑1 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           <  SRR4302532.1460181/94‑1 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           <  SRR4302532.4618822/94‑1 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           <  SRR4302532.6200761/94‑1 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           <  SRR4302532.5218654/94‑1 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           >  SRR4302532.1422434/1‑94 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGACTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           <  SRR4302532.4265083/94‑1 (MQ=60)

                                     CGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCG                                                          <  SRR4302532.4003618/94‑1 (MQ=60)

                                     CGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCG                                                          <  SRR4302532.2235130/94‑1 (MQ=60)

                                       TTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTT                                                        <  SRR4302532.5753164/94‑1 (MQ=60)

                                        TATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTT                                                       >  SRR4302532.6269200/1‑94 (MQ=60)

                                         ATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTC                                                      >  SRR4302532.6187779/1‑94 (MQ=60)

                                          TTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCT                                                     >  SRR4302532.4384929/1‑94 (MQ=60)

                                           TGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTCAATTTGGCTAAATCGTTTCTC                                                    >  SRR4302532.1570726/1‑94 (MQ=60)

                                            GAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCT                                                   <  SRR4302532.4323559/94‑1 (MQ=60)

                                            GAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCT                                                   <  SRR4302532.4323560/94‑1 (MQ=60)

                                            GAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCT                                                   >  SRR4302532.3915251/1‑94 (MQ=60)

                                             AGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTA                                                  >  SRR4302532.1151256/1‑94 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAC                                                 >  SRR4302532.4115106/1‑94 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAC                                                 <  SRR4302532.1835894/94‑1 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAC                                                 >  SRR4302532.2030373/1‑94 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAC                                                 <  SRR4302532.2632580/94‑1 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATAGTTTCTCTAC                                                 >  SRR4302532.6319040/1‑94 (MQ=60)

                                               TTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACG                                                <  SRR4302532.2327017/94‑1 (MQ=60)

                                               TTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACG                                                <  SRR4302532.267672/94‑1 (MQ=60)

                                                  ACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCT                                             >  SRR4302532.101104/1‑94 (MQ=60)

                                                  ACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCT                                             <  SRR4302532.4477829/94‑1 (MQ=60)

                                                    AGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTT                                           >  SRR4302532.4739920/1‑94 (MQ=60)

                                                       AACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTA                                        >  SRR4302532.2004143/1‑94 (MQ=60)

                                                         CAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAAT                                      <  SRR4302532.1453984/94‑1 (MQ=60)

                                                          AGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATC                                     <  SRR4302532.6343759/94‑1 (MQ=60)

                                                           GGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCC                                    <  SRR4302532.5377620/94‑1 (MQ=60)

                                                           GGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCC                                    <  SRR4302532.6164732/94‑1 (MQ=60)

                                                             CGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAG                                  >  SRR4302532.4589840/1‑94 (MQ=60)

                                                             CGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAG                                  >  SRR4302532.4589841/1‑94 (MQ=60)

                                                              GCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGC                                 >  SRR4302532.3751503/1‑94 (MQ=60)

                                                               CAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCA                                <  SRR4302532.144528/94‑1 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               <  SRR4302532.1508920/94‑1 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               <  SRR4302532.4902246/94‑1 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               >  SRR4302532.3706339/1‑94 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               <  SRR4302532.3615180/94‑1 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGACTTTGTAATCCAGCAG                               <  SRR4302532.1908020/94‑1 (MQ=60)

                                                                 AGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTTTACGCCTTTGTAATCCAGCAGA                              >  SRR4302532.3395190/1‑94 (MQ=60)

                                                                 AGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGA                              >  SRR4302532.2477099/1‑94 (MQ=60)

                                                                  GAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGAT                             <  SRR4302532.2059409/94‑1 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            <  SRR4302532.1528606/94‑1 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            <  SRR4302532.5259823/94‑1 (MQ=60)

                                                                    AAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGA                           >  SRR4302532.2793856/1‑94 (MQ=60)

                                                                    AAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGT‑ATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302532.1648033/94‑1 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302532.5904416/94‑1 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302532.3391919/94‑1 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302532.3254543/94‑1 (MQ=60)

                                                                       GCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGC                        >  SRR4302532.242152/1‑94 (MQ=60)

                                                                        CCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCT                       >  SRR4302532.6049549/1‑94 (MQ=60)

                                                                        CCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCT                       >  SRR4302532.6049548/1‑94 (MQ=60)

                                                                         CAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTC                      >  SRR4302532.4350751/1‑94 (MQ=60)

                                                                         CAACCATTAAGGCTACCAGCTACCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTATCTACGCCTTTGTAATCCAGCAGATGATGCTC                      >  SRR4302532.3404683/1‑94 (MQ=60)

                                                                          AACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCC                     >  SRR4302532.1800069/1‑94 (MQ=60)

                                                                          AACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCC                     >  SRR4302532.3760512/1‑94 (MQ=60)

                                                                          AACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCC                     >  SRR4302532.1307849/1‑94 (MQ=60)

                                                                           ACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCG                    >  SRR4302532.3202950/1‑94 (MQ=60)

                                                                           ACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCG                    <  SRR4302532.1865979/94‑1 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGC                   >  SRR4302532.1135916/1‑94 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGC                   >  SRR4302532.5212551/1‑94 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGC                   >  SRR4302532.1136315/1‑94 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGA                   >  SRR4302532.5442924/1‑94 (MQ=60)

                                                                             CATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCA                  >  SRR4302532.5306386/1‑94 (MQ=60)

                                                                              ATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAA                 >  SRR4302532.780119/1‑94 (MQ=60)

                                                                                TAAGCCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGA               >  SRR4302532.2479848/1‑94 (MQ=60)

                                                                                 AAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAA              <  SRR4302532.3448011/94‑1 (MQ=60)

                                                                                 AAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAA              >  SRR4302532.3017948/1‑94 (MQ=60)

                                                                                 AAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAA              <  SRR4302532.769577/94‑1 (MQ=60)

                                                                                  AGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAT             >  SRR4302532.5718666/1‑94 (MQ=60)

                                                                                   GGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATC            >  SRR4302532.922348/1‑94 (MQ=60)

                                                                                   GGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATC            <  SRR4302532.1204665/94‑1 (MQ=60)

                                                                                   GGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATC            >  SRR4302532.353800/1‑94 (MQ=60)

                                                                                   GGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATC            >  SRR4302532.2544242/1‑94 (MQ=60)

                                                                                    GCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCA           <  SRR4302532.93172/94‑1 (MQ=60)

                                                                                    GCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCA           <  SRR4302532.1359313/94‑1 (MQ=60)

                                                                                      TACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACA         <  SRR4302532.4833648/94‑1 (MQ=60)

                                                                                      TACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACA         >  SRR4302532.5140836/1‑94 (MQ=60)

                                                                                       ACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAA        >  SRR4302532.6029801/1‑94 (MQ=60)

                                                                                        CCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAT       <  SRR4302532.5853660/94‑1 (MQ=60)

                                                                                        CCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAT       >  SRR4302532.4287040/1‑94 (MQ=60)

                                                                                        CCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAT       <  SRR4302532.3727189/94‑1 (MQ=60)

                                                                                        CCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAT       >  SRR4302532.4057586/1‑94 (MQ=60)

                                                                                         CAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG      >  SRR4302532.64891/1‑94 (MQ=60)

                                                                                         CAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG      >  SRR4302532.6332656/1‑94 (MQ=60)

                                                                                          AGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT     >  SRR4302532.1919070/1‑94 (MQ=60)

                                                                                          AGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT     >  SRR4302532.1269905/1‑94 (MQ=60)

                                                                                          AGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT     >  SRR4302532.3767757/1‑94 (MQ=60)

                                                                                             TATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  <  SRR4302532.2281149/94‑1 (MQ=60)

                                                                                             TATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  <  SRR4302532.764974/94‑1 (MQ=60)

                                                                                             TATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCCTTGTAATCCAGCAGATGATGCTCCGCATGAATCACAATGTGCT  >  SRR4302532.5681615/1‑94 (MQ=60)

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  >  NC_000913/4599985‑4600171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`