Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,608,595 2 bp→GC intergenic (+23/‑369) prfC → / → osmY peptide chain release factor RF‑3/OsmY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,5950CG100.0% 866.8 / NA 245intergenic (+23/‑370)prfC/osmYpeptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (102/143);  total (102/143)
*NC_0009134,608,5960GC100.0% 889.2 / NA 245intergenic (+24/‑369)prfC/osmYpeptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (102/143);  total (102/143)

Only 100 of 247 total aligned reads displayed.

TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA  >  NC_000913/4608508‑4608682
                                                                                       ||                                                                                      
  aaCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                  >  1:5612193/1‑93 (MQ=255)
  aaCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                  <  2:2143089/93‑1 (MQ=255)
  aaCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGCGCGCGGTAGCCGCTgc                                                                                  >  2:192356/1‑93 (MQ=255)
      tGCGCCTGGCAGAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                              <  1:679369/93‑1 (MQ=255)
           cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCATACCCGCGAGCATTAATTCATTCCCCCCGGACGCCGTAGCCGCTGCGCCCTGTCa                                                                         >  2:2780241/1‑93 (MQ=255)
             ggCACAGGAAAGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAt                                                                       <  1:5310706/93‑1 (MQ=255)
                  aGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTccc                                                                  <  1:3832796/93‑1 (MQ=255)
                   ggAACGTTATCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                 <  2:2633321/93‑1 (MQ=255)
                   ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                 <  1:5234908/93‑1 (MQ=255)
                   ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                 <  2:642840/93‑1 (MQ=255)
                   ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                 >  1:1543237/1‑93 (MQ=255)
                     aaCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttc                                                               <  1:3424299/93‑1 (MQ=255)
                     aaCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttc                                                               <  2:3642791/93‑1 (MQ=255)
                      aCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcc                                                              <  2:4701262/93‑1 (MQ=255)
                       cGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcct                                                             <  2:1831196/93‑1 (MQ=255)
                        gTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcctt                                                            >  1:1837973/1‑93 (MQ=255)
                        gTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcctt                                                            >  2:1043788/1‑93 (MQ=255)
                         ttATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCtta                                                           <  1:4822257/93‑1 (MQ=255)
                         ttATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCtta                                                           <  2:2486425/93‑1 (MQ=255)
                         ttATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCtta                                                           <  1:4577045/93‑1 (MQ=255)
                         ttATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCtta                                                           <  2:1430306/93‑1 (MQ=255)
                          tATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttat                                                          >  1:1385269/1‑93 (MQ=255)
                          tATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttat                                                          >  2:6094768/1‑93 (MQ=255)
                          tATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttat                                                          <  1:1596657/93‑1 (MQ=255)
                           aTCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatt                                                         >  2:1619582/1‑93 (MQ=255)
                            tCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        <  2:5856230/93‑1 (MQ=255)
                            tCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        <  2:3046473/93‑1 (MQ=255)
                            tCCGGACGTACAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        <  1:2857078/93‑1 (MQ=255)
                              cGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGc                                                      <  1:5636555/93‑1 (MQ=255)
                                gACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCg                                                    >  1:1162767/1‑93 (MQ=255)
                                 aCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGc                                                   <  2:2722599/93‑1 (MQ=255)
                                 aCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGc                                                   <  1:1231796/93‑1 (MQ=255)
                                  cGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCt                                                  <  2:3990517/93‑1 (MQ=255)
                                   gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCtt                                                 <  2:5013083/93‑1 (MQ=255)
                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                >  1:3582567/1‑93 (MQ=255)
                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                >  1:251587/1‑93 (MQ=255)
                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                <  1:264174/93‑1 (MQ=255)
                                    ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                >  2:1133034/1‑93 (MQ=255)
                                    tgcAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                <  1:829693/91‑1 (MQ=255)
                                     tCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAc                                               <  2:4462150/93‑1 (MQ=255)
                                      cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                              <  2:5384423/93‑1 (MQ=255)
                                      cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                              >  2:1254665/1‑93 (MQ=255)
                                       aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                             <  1:451991/93‑1 (MQ=255)
                                         ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                           <  2:3435505/93‑1 (MQ=255)
                                         ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                           <  1:6028142/93‑1 (MQ=255)
                                         ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                           >  2:2209167/1‑93 (MQ=255)
                                          tCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                          >  2:129723/1‑93 (MQ=255)
                                           ccaccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATg                                         <  2:5552158/93‑1 (MQ=255)
                                            caccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                        >  1:2818420/1‑93 (MQ=255)
                                            caccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                        <  2:291771/93‑1 (MQ=255)
                                            caccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                        <  1:174405/93‑1 (MQ=255)
                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGttt                                      >  2:2458675/1‑92 (MQ=255)
                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      <  2:4948703/93‑1 (MQ=255)
                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      <  1:273549/93‑1 (MQ=255)
                                              ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      <  2:2557399/93‑1 (MQ=255)
                                               caGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTACTTATTAGCCGCTTACGGAATGTTCt                                     >  1:1954187/1‑93 (MQ=255)
                                                 gACCCGCGAGCATTAATTCTTGCCTCCCGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTa                                   >  1:3950684/1‑93 (MQ=255)
                                                 gACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTa                                   <  2:4069099/93‑1 (MQ=255)
                                                 gACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTa                                   <  1:335656/93‑1 (MQ=255)
                                                   cccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaa                                 <  1:367211/93‑1 (MQ=255)
                                                   cccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaa                                 >  1:6094941/1‑93 (MQ=255)
                                                    ccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                                <  2:5736950/93‑1 (MQ=255)
                                                    ccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                                <  1:4994485/93‑1 (MQ=255)
                                                     cgcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAc                               <  1:2266339/93‑1 (MQ=255)
                                                      gcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACa                              <  2:3810238/93‑1 (MQ=255)
                                                      gcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACa                              <  1:4211344/93‑1 (MQ=255)
                                                       cgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAt                             <  1:5855875/93‑1 (MQ=255)
                                                       cgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAt                             >  1:5691492/1‑93 (MQ=255)
                                                        gAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAtt                            >  1:5745846/1‑93 (MQ=255)
                                                        gAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAtt                            <  1:3722204/93‑1 (MQ=255)
                                                        gAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGGTCTTAAAACAtt                            >  2:4495861/1‑93 (MQ=255)
                                                        gAGCATTAATTCTTGCCTCCAGGGAGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTCCGGAATGTTCTTAAAACAtt                            >  2:2094789/1‑93 (MQ=255)
                                                         aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           <  2:4306144/93‑1 (MQ=255)
                                                         aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           <  1:4308514/93‑1 (MQ=255)
                                                           cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                         <  2:4027792/93‑1 (MQ=255)
                                                           cATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAc                         <  2:872774/93‑1 (MQ=255)
                                                            aTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                        >  1:3178661/1‑93 (MQ=255)
                                                             ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtt                       <  2:3242676/93‑1 (MQ=255)
                                                              tAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACttt                      <  2:4338883/93‑1 (MQ=255)
                                                               aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtttt                     <  2:1475090/93‑1 (MQ=255)
                                                                 ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   <  2:3755802/93‑1 (MQ=255)
                                                                 ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   >  2:602250/1‑93 (MQ=255)
                                                                 ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   >  2:5156852/1‑93 (MQ=255)
                                                                    ttGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTa                >  2:4616739/1‑93 (MQ=255)
                                                                      gCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATg              <  1:2071449/93‑1 (MQ=255)
                                                                       ccTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGt             >  2:1154529/1‑93 (MQ=255)
                                                                        cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtt            <  2:4429592/93‑1 (MQ=255)
                                                                        cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtt            <  1:5389918/93‑1 (MQ=255)
                                                                         tCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGttt           >  2:4149573/1‑93 (MQ=255)
                                                                          ccAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtttt          <  2:3227211/93‑1 (MQ=255)
                                                                          ccAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtttt          <  1:442685/93‑1 (MQ=255)
                                                                           cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc         >  2:2344977/1‑93 (MQ=255)
                                                                           cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc         >  1:5077552/1‑93 (MQ=255)
                                                                           cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc         <  2:41943/93‑1 (MQ=255)
                                                                           cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc         <  2:38646/93‑1 (MQ=255)
                                                                            aGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCg        >  2:3201926/1‑93 (MQ=255)
                                                                             gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc       <  2:4790314/93‑1 (MQ=255)
                                                                              ggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCt      <  2:3586568/93‑1 (MQ=255)
                                                                                cgcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGa    <  2:5313152/93‑1 (MQ=255)
                                                                                  cgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGata  >  1:4926773/1‑93 (MQ=255)
                                                                                       ||                                                                                      
TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA  >  NC_000913/4608508‑4608682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608502‑4608684
                                                                                             |                                                                                         
GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAG                                                                                           <  SRR4302532.2735835/94‑1 (MQ=60)
     GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      <  SRR4302532.1682900/94‑1 (MQ=60)
     GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      >  SRR4302532.4869055/1‑94 (MQ=60)
       CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                    >  SRR4302532.5806951/1‑94 (MQ=60)
            TGCGCCTGGCAGAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               <  SRR4302532.702737/94‑1 (MQ=60)
            TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               <  SRR4302532.1602540/94‑1 (MQ=60)
             GCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG                                                                              <  SRR4302532.4544360/94‑1 (MQ=60)
               GCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTC                                                                            <  SRR4302532.376962/94‑1 (MQ=60)
                CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302532.2838459/1‑94 (MQ=60)
                   GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        >  SRR4302532.5246034/1‑94 (MQ=60)
                   GGCACAGGAAAGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302532.5494354/94‑1 (MQ=60)
                     CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      <  SRR4302532.3478147/94‑1 (MQ=60)
                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302532.1595382/1‑94 (MQ=60)
                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302532.1708602/1‑94 (MQ=60)
                        AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   <  SRR4302532.3962203/94‑1 (MQ=60)
                         GGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTT                                                                  <  SRR4302532.5147535/94‑1 (MQ=60)
                         GGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTT                                                                  <  SRR4302532.5415433/94‑1 (MQ=60)
                           AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCC                                                                <  SRR4302532.3539019/94‑1 (MQ=60)
                           AACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCC                                                                >  SRR4302532.1236474/1‑94 (MQ=60)
                             CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              >  SRR4302532.1899052/1‑94 (MQ=60)
                              GTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTA                                                             >  SRR4302532.5265708/1‑94 (MQ=60)
                               TTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                            >  SRR4302532.1431688/1‑94 (MQ=60)
                               TTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                            <  SRR4302532.4988262/94‑1 (MQ=60)
                               TTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTAT                                                            <  SRR4302532.4733120/94‑1 (MQ=60)
                                TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           <  SRR4302532.1650705/94‑1 (MQ=60)
                                 ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302532.444888/94‑1 (MQ=60)
                                  TCCGGACGTACAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                         <  SRR4302532.2952618/94‑1 (MQ=60)
                                   CCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGC                                                        >  SRR4302532.2012682/1‑94 (MQ=60)
                                    CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       <  SRR4302532.5832365/94‑1 (MQ=60)
                                    CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       >  SRR4302532.2902726/1‑94 (MQ=60)
                                    CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       >  SRR4302532.1304464/1‑94 (MQ=60)
                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      >  SRR4302532.1201611/1‑94 (MQ=60)
                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      <  SRR4302532.5825732/94‑1 (MQ=60)
                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      >  SRR4302532.2634146/1‑94 (MQ=60)
                                     GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      >  SRR4302532.4847030/1‑94 (MQ=60)
                                       ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    <  SRR4302532.1273010/94‑1 (MQ=60)
                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302532.1824921/1‑94 (MQ=60)
                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302532.3702413/1‑94 (MQ=60)
                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302532.857327/1‑94 (MQ=60)
                                         GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302532.260337/1‑94 (MQ=60)
                                          TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302532.273334/94‑1 (MQ=60)
                                          TGCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302532.858098/94‑1 (MQ=60)
                                             AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                              <  SRR4302532.467703/94‑1 (MQ=60)
                                              GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAA                                             >  SRR4302532.2410155/1‑94 (MQ=60)
                                               TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302532.6236338/94‑1 (MQ=60)
                                               TCCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302532.553105/94‑1 (MQ=60)
                                                 CCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGT                                          >  SRR4302532.2912754/1‑94 (MQ=60)
                                                  CACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                         <  SRR4302532.180236/94‑1 (MQ=60)
                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302532.283020/94‑1 (MQ=60)
                                                    CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTACTTATTAGCCGCTTACGGAATGTTCT                                       >  SRR4302532.2019106/1‑94 (MQ=60)
                                                      AGACCCGCGAGCATTAATTCTTGCCTCCCGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     >  SRR4302532.4083825/1‑94 (MQ=60)
                                                      AGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     >  SRR4302532.3661034/1‑94 (MQ=60)
                                                      AGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     >  SRR4302532.3047712/1‑94 (MQ=60)
                                                      AGACCCGCGAGAATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     >  SRR4302532.6278218/1‑94 (MQ=60)
                                                       GACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAA                                    >  SRR4302532.6002824/1‑94 (MQ=60)
                                                       GACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAA                                    <  SRR4302532.347290/94‑1 (MQ=60)
                                                       AACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAA                                    <  SRR4302532.5491262/94‑1 (MQ=60)
                                                        ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   <  SRR4302532.5038302/94‑1 (MQ=60)
                                                        ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   >  SRR4302532.4059000/1‑94 (MQ=60)
                                                        ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   >  SRR4302532.6305331/1‑94 (MQ=60)
                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTCAAA                                  >  SRR4302532.2755677/1‑94 (MQ=60)
                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAGAA                                  <  SRR4302532.6020901/94‑1 (MQ=60)
                                                         CCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA                                  <  SRR4302532.379972/94‑1 (MQ=60)
                                                          CCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAAC                                 <  SRR4302532.5165675/94‑1 (MQ=60)
                                                           CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACA                                <  SRR4302532.2341555/94‑1 (MQ=60)
                                                           CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACA                                <  SRR4302532.2341553/94‑1 (MQ=60)
                                                            GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               <  SRR4302532.4352707/94‑1 (MQ=60)
                                                            GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               >  SRR4302532.5876325/1‑94 (MQ=60)
                                                            GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               >  SRR4302532.5890093/1‑94 (MQ=60)
                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              >  SRR4302532.5704811/1‑94 (MQ=60)
                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              <  SRR4302532.6058941/94‑1 (MQ=60)
                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              >  SRR4302532.2303621/1‑94 (MQ=60)
                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              >  SRR4302532.918606/1‑94 (MQ=60)
                                                             CGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATT                              >  SRR4302532.5945860/1‑94 (MQ=60)
                                                              GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             <  SRR4302532.3354399/94‑1 (MQ=60)
                                                              GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             <  SRR4302532.3847186/94‑1 (MQ=60)
                                                               AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302532.4454184/94‑1 (MQ=60)
                                                               AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302532.4598821/94‑1 (MQ=60)
                                                               AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            >  SRR4302532.2696622/1‑94 (MQ=60)
                                                                GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302532.2057026/94‑1 (MQ=60)
                                                                 CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          >  SRR4302532.3284219/1‑94 (MQ=60)
                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         >  SRR4302532.5121688/1‑94 (MQ=60)
                                                                  ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         >  SRR4302532.641165/1‑94 (MQ=60)
                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        >  SRR4302532.1521779/1‑94 (MQ=60)
                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        >  SRR4302532.4696625/1‑94 (MQ=60)
                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        <  SRR4302532.4586352/94‑1 (MQ=60)
                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        <  SRR4302532.4005817/94‑1 (MQ=60)
                                                                   TTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTT                        <  SRR4302532.4248172/94‑1 (MQ=60)
                                                                    TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAACACATTCACTTTT                       <  SRR4302532.3811956/94‑1 (MQ=60)
                                                                    TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       <  SRR4302532.3151332/94‑1 (MQ=60)
                                                                    TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       <  SRR4302532.5834469/94‑1 (MQ=60)
                                                                    TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       <  SRR4302532.5760973/94‑1 (MQ=60)
                                                                     AATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTG                      <  SRR4302532.176087/94‑1 (MQ=60)
                                                                     AATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTG                      <  SRR4302532.3845593/94‑1 (MQ=60)
                                                                     AATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTG                      <  SRR4302532.4384055/94‑1 (MQ=60)
                                                                      ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGC                     >  SRR4302532.1919869/1‑94 (MQ=60)
                                                                        TCTTGCCTCCAGGGCGCGGTAGCTGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                   <  SRR4302532.3614987/94‑1 (MQ=60)
                                                                        TCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                   >  SRR4302532.5370402/1‑94 (MQ=60)
                                                                         CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  >  SRR4302532.3860571/1‑94 (MQ=60)
                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               <  SRR4302532.2140496/94‑1 (MQ=60)
                                                                            GCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGT               <  SRR4302532.737811/94‑1 (MQ=60)
                                                                              CTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTT             <  SRR4302532.5576637/94‑1 (MQ=60)
                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTGCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           >  SRR4302532.6131539/1‑94 (MQ=60)
                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           >  SRR4302532.5252116/1‑94 (MQ=60)
                                                                                CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302532.457990/94‑1 (MQ=60)
                                                                                CCAGGGCGAGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           <  SRR4302532.293698/94‑1 (MQ=60)
                                                                                  AGGGCGCGGTCGCCGCTGCGCCCTGCCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302532.2449416/1‑94 (MQ=60)
                                                                                   GGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCT        >  SRR4302532.1450362/1‑94 (MQ=60)
                                                                                    GGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTG       <  SRR4302532.5891600/94‑1 (MQ=60)
                                                                                      CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT     <  SRR4302532.627246/94‑1 (MQ=60)
                                                                                       GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA    >  SRR4302532.3290623/1‑94 (MQ=60)
                                                                                       GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA    >  SRR4302532.5095969/1‑94 (MQ=60)
                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  SRR4302532.3808481/1‑94 (MQ=60)
                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  <  SRR4302532.5014065/94‑1 (MQ=60)
                                                                                         GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  <  SRR4302532.1510736/94‑1 (MQ=60)
                                                                                             |                                                                                         
GCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608502‑4608684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑