Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,608,595 2 bp→GC intergenic (+23/‑369) prfC → / → osmY peptide chain release factor RF‑3/OsmY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,5950CG100.0% 897.6 / NA 247intergenic (+23/‑370)prfC/osmYpeptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (130/117);  total (130/117)
*NC_0009134,608,5960GC100.0% 908.4 / NA 247intergenic (+24/‑369)prfC/osmYpeptide chain release factor RF‑3/OsmY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (130/117);  total (130/117)

Only 100 of 254 total aligned reads displayed.

CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA  >  NC_000913/4608509‑4608682
                                                                                      ||                                                                                      
 aaCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                  <  2:5082431/93‑1 (MQ=255)
 aaCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgc                                                                                  <  1:4740629/93‑1 (MQ=255)
  aCCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgcg                                                                                 >  2:6593124/1‑93 (MQ=255)
   ccTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgcgc                                                                                >  1:657014/1‑93 (MQ=255)
     tGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                              <  1:6643232/93‑1 (MQ=255)
     tGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                              <  1:6494268/93‑1 (MQ=255)
     tGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGccc                                                                              <  1:2392241/93‑1 (MQ=255)
      gcgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCt                                                                             <  2:1581028/93‑1 (MQ=255)
      gcgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCt                                                                             >  1:40381/1‑93 (MQ=255)
       cgcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTg                                                                            >  2:2060595/1‑93 (MQ=255)
        gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGt                                                                           <  2:184336/93‑1 (MQ=255)
        gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGt                                                                           >  2:590521/1‑93 (MQ=255)
        gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGt                                                                           <  1:2762804/93‑1 (MQ=255)
        gcCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGGAGCCGCTGCGCCCTGt                                                                           >  2:4282554/1‑93 (MQ=255)
         ccTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTc                                                                          <  1:4845594/93‑1 (MQ=255)
         ccTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTc                                                                          >  2:6112278/1‑93 (MQ=255)
          cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCa                                                                         >  1:4312454/1‑93 (MQ=255)
          cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCa                                                                         >  1:5250228/1‑93 (MQ=255)
          cTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCa                                                                         >  1:932628/1‑93 (MQ=255)
           tGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCaa                                                                        <  2:705791/93‑1 (MQ=255)
           tGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGCTAGCCGCTGCGCCCTGTCac                                                                        >  2:4308489/1‑92 (MQ=255)
            ggCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAt                                                                       >  2:531764/1‑93 (MQ=255)
             gCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAtt                                                                      <  1:139990/93‑1 (MQ=255)
              cacaGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAAttt                                                                     >  1:3434471/1‑93 (MQ=255)
                caGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTcc                                                                   >  1:4643614/1‑93 (MQ=255)
                 aGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTccc                                                                  >  1:2614518/1‑93 (MQ=255)
                 aGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTccc                                                                  <  2:3049844/93‑1 (MQ=255)
                  ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                 >  1:2433229/1‑93 (MQ=255)
                  ggAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCcct                                                                 >  2:3844960/1‑93 (MQ=255)
                     aCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcc                                                              <  2:5386963/93‑1 (MQ=255)
                     aCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcc                                                              <  2:3712193/93‑1 (MQ=255)
                     aCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcc                                                              <  1:3282344/93‑1 (MQ=255)
                      cGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcct                                                             <  1:1454122/93‑1 (MQ=255)
                       gTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCccttcctt                                                            >  2:6199180/1‑93 (MQ=255)
                         tATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttat                                                          <  2:4375363/93‑1 (MQ=255)
                         tATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttat                                                          <  1:1643172/93‑1 (MQ=255)
                         tATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttat                                                          >  2:1942657/1‑93 (MQ=255)
                           tCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        >  2:5520308/1‑93 (MQ=255)
                           tCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        <  1:2412354/93‑1 (MQ=255)
                           tCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        <  1:6136188/93‑1 (MQ=255)
                           tCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCttatta                                                        <  2:2582467/93‑1 (MQ=255)
                            caggACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAg                                                       <  2:2756326/91‑1 (MQ=255)
                              ggACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGcc                                                     >  1:1538276/1‑93 (MQ=255)
                               gACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCg                                                    >  1:1341282/1‑93 (MQ=255)
                                aCGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGc                                                   >  1:1696805/1‑93 (MQ=255)
                                 cGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCt                                                  <  1:4790830/93‑1 (MQ=255)
                                  gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCtt                                                 >  1:2379656/1‑93 (MQ=255)
                                  gTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCtt                                                 <  2:446234/93‑1 (MQ=255)
                                   ttCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTa                                                >  2:246717/1‑93 (MQ=255)
                                     cAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACg                                              <  2:3415860/93‑1 (MQ=255)
                                      aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                             <  2:2537281/93‑1 (MQ=255)
                                      aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                             <  2:1829701/93‑1 (MQ=255)
                                      aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                             >  2:1169816/1‑93 (MQ=255)
                                      aGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACgg                                             <  1:5794887/93‑1 (MQ=255)
                                       gTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGa                                            >  1:106338/1‑93 (MQ=255)
                                        ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACggca                                           >  1:1272383/1‑91 (MQ=255)
                                        ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                           >  1:2628269/1‑93 (MQ=255)
                                        ttCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGaa                                           <  1:3536335/93‑1 (MQ=255)
                                         tCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                          <  2:6444051/93‑1 (MQ=255)
                                         tCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAt                                          >  1:4274316/1‑93 (MQ=255)
                                           caccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                        <  2:47727/93‑1 (MQ=255)
                                           caccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGt                                        <  2:4803839/93‑1 (MQ=255)
                                            accaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGtt                                       >  2:1872643/1‑93 (MQ=255)
                                             ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      <  2:109375/93‑1 (MQ=255)
                                             ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      >  1:1809269/1‑93 (MQ=255)
                                             ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      <  2:3169606/93‑1 (MQ=255)
                                             ccaGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTc                                      <  1:487069/93‑1 (MQ=255)
                                                 aCCCGCGAGCATTAATTCTTGCCTCCAGGTCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaa                                  >  2:5147535/1‑93 (MQ=255)
                                                  cccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaa                                 >  1:5186929/1‑93 (MQ=255)
                                                   ccGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTaaaa                                <  2:5595041/93‑1 (MQ=255)
                                                     gcgAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACa                              <  2:2255368/93‑1 (MQ=255)
                                                        aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           <  2:6368407/93‑1 (MQ=255)
                                                        aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           >  2:4599250/1‑93 (MQ=255)
                                                        aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           >  2:820585/1‑93 (MQ=255)
                                                        aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           >  2:267449/1‑93 (MQ=255)
                                                        aGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTc                           >  1:550239/1‑93 (MQ=255)
                                                         gCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCa                          >  1:1135989/1‑93 (MQ=255)
                                                         gCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCa                          <  1:3209561/93‑1 (MQ=255)
                                                         gCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCa                          <  1:5869934/93‑1 (MQ=255)
                                                           aTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                        >  1:439947/1‑93 (MQ=255)
                                                           aTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                        >  2:6046341/1‑93 (MQ=255)
                                                           aTTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACt                        <  1:4168286/93‑1 (MQ=255)
                                                            ttAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtt                       >  2:3542948/1‑93 (MQ=255)
                                                              aaTTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACtttt                     <  2:1234659/93‑1 (MQ=255)
                                                               aTTCTTGCCTCCAGGGCGCGGTAGCCTCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTg                    <  2:1653001/93‑1 (MQ=255)
                                                                ttCTTGTCTCCAGGCCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   <  1:2974217/93‑1 (MQ=255)
                                                                ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   <  2:4882843/93‑1 (MQ=255)
                                                                ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   >  1:602774/1‑93 (MQ=255)
                                                                ttCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGc                   <  1:1921476/93‑1 (MQ=255)
                                                                 tCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCt                  <  1:4455836/93‑1 (MQ=255)
                                                                  cTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCtt                 <  1:3848168/93‑1 (MQ=255)
                                                                   ttGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTa                <  2:1364547/93‑1 (MQ=255)
                                                                     gCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATg              <  2:5530838/93‑1 (MQ=255)
                                                                         ccAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtttt          >  1:1653721/1‑93 (MQ=255)
                                                                         ccAGGCCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGtttt          <  2:1494521/93‑1 (MQ=255)
                                                                          cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTc         >  1:3798285/1‑93 (MQ=255)
                                                                            gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc       >  1:2032534/1‑93 (MQ=255)
                                                                            gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGc       >  1:5933206/1‑93 (MQ=255)
                                                                              gcgcGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTg     >  2:2889581/1‑93 (MQ=255)
                                                                               cgcgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGa    <  1:3609081/93‑1 (MQ=255)
                                                                                      ||                                                                                      
CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA  >  NC_000913/4608509‑4608682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608507‑4608684
                                                                                        |                                                                                         
GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      <  SRR4302547.5024236/94‑1 (MQ=60)
GTCAACCTGCGCCTGACACAGCAACGTTCTCCGGACGTTAAGTTCCACCAGACCGGCGCGAATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCT                                                                                      <  SRR4302547.6342546/94‑1 (MQ=60)
 TCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTG                                                                                     >  SRR4302547.2030879/1‑94 (MQ=60)
  CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                    <  SRR4302547.1723566/94‑1 (MQ=60)
  CAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGC                                                                                    <  SRR4302547.5107075/94‑1 (MQ=60)
   AACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCG                                                                                   <  SRR4302547.4838880/94‑1 (MQ=60)
    ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGC                                                                                  >  SRR4302547.669117/1‑94 (MQ=60)
    ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGC                                                                                  >  SRR4302547.6359407/1‑94 (MQ=60)
    ACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGC                                                                                  >  SRR4302547.2867883/1‑94 (MQ=60)
      CTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCC                                                                                >  SRR4302547.3661974/1‑94 (MQ=60)
       TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               <  SRR4302547.6781538/94‑1 (MQ=60)
       TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               <  SRR4302547.6629458/94‑1 (MQ=60)
       TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               <  SRR4302547.2440703/94‑1 (MQ=60)
       TGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCT                                                                               >  SRR4302547.41126/1‑94 (MQ=60)
        GCGCCTGGCACAGTAACGTTATCCGGACGTTCAGTTCCACCGTACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG                                                                              <  SRR4302547.1109388/94‑1 (MQ=60)
          GCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTC                                                                            <  SRR4302547.2818173/94‑1 (MQ=60)
           CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302547.4401190/1‑94 (MQ=60)
           CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           <  SRR4302547.4946015/94‑1 (MQ=60)
           CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302547.5358319/1‑94 (MQ=60)
           CCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCA                                                                           >  SRR4302547.950660/1‑94 (MQ=60)
            CTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAA                                                                          >  SRR4302547.3975287/1‑94 (MQ=60)
              GGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATT                                                                        <  SRR4302547.5983779/94‑1 (MQ=60)
               GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTT                                                                       >  SRR4302547.3504476/1‑94 (MQ=60)
               GCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTT                                                                       <  SRR4302547.142522/94‑1 (MQ=60)
                CACAGGATCGTTATCCGGACGTTCAGTTCCACCAGCCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      <  SRR4302547.5902462/94‑1 (MQ=60)
                CACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTC                                                                      <  SRR4302547.663067/94‑1 (MQ=60)
                 ACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCC                                                                     >  SRR4302547.4739788/1‑94 (MQ=60)
                  CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    >  SRR4302547.4603712/1‑94 (MQ=60)
                  CAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCC                                                                    >  SRR4302547.2667208/1‑94 (MQ=60)
                   AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCTCT                                                                   <  SRR4302547.6225886/94‑1 (MQ=60)
                   AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302547.2482453/1‑94 (MQ=60)
                   AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302547.1518098/1‑94 (MQ=60)
                   AGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCT                                                                   >  SRR4302547.6145321/1‑94 (MQ=60)
                     GACCGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTC                                                                 <  SRR4302547.5075916/94‑1 (MQ=60)
                     GAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTC                                                                 >  SRR4302547.5964149/1‑94 (MQ=60)
                       ACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCT                                                               <  SRR4302547.3348153/94‑1 (MQ=60)
                        CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              >  SRR4302547.2440737/1‑94 (MQ=60)
                        CGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTT                                                              <  SRR4302547.1483191/94‑1 (MQ=60)
                         GTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTA                                                             >  SRR4302547.1208925/1‑94 (MQ=60)
                           TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           >  SRR4302547.4351776/1‑94 (MQ=60)
                           TATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATT                                                           <  SRR4302547.1675637/94‑1 (MQ=60)
                            ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          <  SRR4302547.5305707/94‑1 (MQ=60)
                            ATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTA                                                          >  SRR4302547.4232226/1‑94 (MQ=60)
                             TCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                         <  SRR4302547.2461193/94‑1 (MQ=60)
                             TCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAG                                                         <  SRR4302547.6263478/94‑1 (MQ=60)
                               CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       >  SRR4302547.4345205/1‑94 (MQ=60)
                               CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       <  SRR4302547.193040/94‑1 (MQ=60)
                               CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       <  SRR4302547.1831676/94‑1 (MQ=60)
                               CGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCC                                                       >  SRR4302547.1568901/1‑94 (MQ=60)
                                GGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCG                                                      >  SRR4302547.1368229/1‑94 (MQ=60)
                                 GACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGC                                                     >  SRR4302547.1730168/1‑94 (MQ=60)
                                 GACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGC                                                     <  SRR4302547.6724/94‑1 (MQ=60)
                                 GACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGC                                                     >  SRR4302547.3874133/1‑94 (MQ=60)
                                  ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    <  SRR4302547.3698699/94‑1 (MQ=60)
                                  ACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCT                                                    >  SRR4302547.446406/1‑94 (MQ=60)
                                   CTTTCAGTTCCCCCAGACCGTCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   <  SRR4302547.3222234/94‑1 (MQ=60)
                                   CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   <  SRR4302547.4890096/94‑1 (MQ=60)
                                   CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   <  SRR4302547.3980357/94‑1 (MQ=60)
                                   CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   >  SRR4302547.2427869/1‑94 (MQ=60)
                                   CGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTT                                                   <  SRR4302547.171234/94‑1 (MQ=60)
                                    GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  >  SRR4302547.4735927/1‑94 (MQ=60)
                                    GTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTA                                                  <  SRR4302547.1395685/94‑1 (MQ=60)
                                     TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 >  SRR4302547.4917446/1‑94 (MQ=60)
                                     TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302547.2453374/94‑1 (MQ=60)
                                     TTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTAC                                                 <  SRR4302547.2435326/94‑1 (MQ=60)
                                        AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                              <  SRR4302547.5914754/94‑1 (MQ=60)
                                        AGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGA                                              >  SRR4302547.108279/1‑94 (MQ=60)
                                         GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGCA                                             >  SRR4302547.1298002/1‑94 (MQ=60)
                                         GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAA                                             >  SRR4302547.2681211/1‑94 (MQ=60)
                                         GTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAA                                             <  SRR4302547.2288100/94‑1 (MQ=60)
                                          TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            >  SRR4302547.6708795/1‑94 (MQ=60)
                                          TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            <  SRR4302547.3608537/94‑1 (MQ=60)
                                          TTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAAT                                            >  SRR4302547.4362083/1‑94 (MQ=60)
                                             CACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                         <  SRR4302547.2461392/94‑1 (MQ=60)
                                             CACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTT                                         >  SRR4302547.4385281/1‑94 (MQ=60)
                                              ACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTC                                        >  SRR4302547.1845020/1‑94 (MQ=60)
                                               CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302547.4402131/94‑1 (MQ=60)
                                               CCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCT                                       <  SRR4302547.495985/94‑1 (MQ=60)
                                                 AGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTA                                     >  SRR4302547.1503118/1‑94 (MQ=60)
                                                  GACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAA                                    <  SRR4302547.4537007/94‑1 (MQ=60)
                                                   TCCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTACA                                   >  SRR4302547.4839645/1‑94 (MQ=60)
                                                   ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   >  SRR4302547.5293683/1‑94 (MQ=60)
                                                   ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   >  SRR4302547.2669105/1‑94 (MQ=60)
                                                   ACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAA                                   <  SRR4302547.1683648/94‑1 (MQ=60)
                                                      CGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACA                                >  SRR4302547.348321/1‑94 (MQ=60)
                                                       GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               <  SRR4302547.6008851/94‑1 (MQ=60)
                                                       GCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACAT                               <  SRR4302547.1446278/94‑1 (MQ=60)
                                                         GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             >  SRR4302547.560169/1‑94 (MQ=60)
                                                         GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             >  SRR4302547.2186331/1‑94 (MQ=60)
                                                         GAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTC                             >  SRR4302547.694357/1‑94 (MQ=60)
                                                          AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            >  SRR4302547.4861979/1‑94 (MQ=60)
                                                          AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            >  SRR4302547.1158926/1‑94 (MQ=60)
                                                          AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302547.1751367/94‑1 (MQ=60)
                                                          AGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTCCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCA                            <  SRR4302547.2805317/94‑1 (MQ=60)
                                                           GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.5991428/94‑1 (MQ=60)
                                                           GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.495886/94‑1 (MQ=60)
                                                           GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.548923/94‑1 (MQ=60)
                                                           GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.4964795/94‑1 (MQ=60)
                                                           GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.3273601/94‑1 (MQ=60)
                                                           GCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.6586297/94‑1 (MQ=60)
                                                           ACATTAATTCTTGCCTCCAGGGCTCTGTAGCAGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCAC                           <  SRR4302547.3503925/94‑1 (MQ=60)
                                                            CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          >  SRR4302547.2583193/1‑94 (MQ=60)
                                                            CATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACT                          >  SRR4302547.448142/1‑94 (MQ=60)
                                                             ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302547.4253184/94‑1 (MQ=60)
                                                             ATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTT                         <  SRR4302547.3636465/94‑1 (MQ=60)
                                                               TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       <  SRR4302547.3304332/94‑1 (MQ=60)
                                                               TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       <  SRR4302547.6063592/94‑1 (MQ=60)
                                                               TAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTT                       >  SRR4302547.160752/1‑94 (MQ=60)
                                                                 ATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGC                     >  SRR4302547.613955/1‑94 (MQ=60)
                                                                  TTCTTGTCTCCAGGCCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    <  SRR4302547.3033405/94‑1 (MQ=60)
                                                                  TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCT                    <  SRR4302547.1959526/94‑1 (MQ=60)
                                                                   TCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTT                   <  SRR4302547.4547938/94‑1 (MQ=60)
                                                                    CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGCATGTTCTTAAAACATTCACTTTTGCTTT                  >  SRR4302547.4790913/1‑94 (MQ=60)
                                                                    CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  <  SRR4302547.132367/94‑1 (MQ=60)
                                                                    CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  <  SRR4302547.3926509/94‑1 (MQ=60)
                                                                    CTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTA                  <  SRR4302547.1261556/94‑1 (MQ=60)
                                                                     TTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTAT                 >  SRR4302547.540836/1‑94 (MQ=60)
                                                                      TGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATG                <  SRR4302547.3585587/94‑1 (MQ=60)
                                                                          TCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTT            >  SRR4302547.1686348/1‑94 (MQ=60)
                                                                           CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           >  SRR4302547.2045104/1‑94 (MQ=60)
                                                                           CCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTC           >  SRR4302547.3875718/1‑94 (MQ=60)
                                                                            CAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCG          >  SRR4302547.3247753/1‑94 (MQ=60)
                                                                             AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302547.6056236/1‑94 (MQ=60)
                                                                             AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302547.2073025/1‑94 (MQ=60)
                                                                             AGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGC         >  SRR4302547.2784225/1‑94 (MQ=60)
                                                                                 CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTACAACATTCACTTTTGCTTATGTTTTCGCTGCT     >  SRR4302547.1676637/1‑94 (MQ=60)
                                                                                 CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT     >  SRR4302547.2634460/1‑94 (MQ=60)
                                                                                 CGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGAT     <  SRR4302547.3682675/94‑1 (MQ=60)
                                                                                  GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCTTTATTAGCCGCTTACGGAATGTTCTTAAACCATTCACTTTTGCTTATGTTTTCGCTGATA    <  SRR4302547.870529/94‑1 (MQ=60)
                                                                                  GCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATA    <  SRR4302547.1414680/94‑1 (MQ=60)
                                                                                   CGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATAT   >  SRR4302547.5092681/1‑94 (MQ=60)
                                                                                    GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  <  SRR4302547.1817989/94‑1 (MQ=60)
                                                                                    GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  SRR4302547.4915650/1‑94 (MQ=60)
                                                                                    GGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  SRR4302547.735556/1‑94 (MQ=60)
                                                                                        |                                                                                         
GTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTACGCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATC  >  NC_000913/4608507‑4608684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑