Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,238	G→T	L133I (CTA→ATA)	yjjI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,238	0	G	T	100.0%	878.7 / NA	234	L133I (CTA→ATA)	yjjI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (126/108); total (126/108)

Only 100 of 242 total aligned reads displayed.

CGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGG  >  NC_000913/4614148‑4614327

cGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATaa                                                                                         >  2:4856074/1‑93 (MQ=255)

 gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAt                                                                                        >  1:5743575/1‑93 (MQ=255)

   tatTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTc                                                                                      >  2:572134/1‑93 (MQ=255)

   tatTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTc                                                                                      >  1:2223254/1‑93 (MQ=255)

     ttGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTa                                                                                    >  2:5510263/1‑93 (MQ=255)

      tGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTaa                                                                                   <  2:4950538/93‑1 (MQ=255)

       ggCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAAc                                                                                  <  2:5884232/93‑1 (MQ=255)

        gCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACa                                                                                 <  2:2246717/93‑1 (MQ=255)

        gCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACa                                                                                 >  1:1763962/1‑93 (MQ=255)

         cGTGCATAAAGGCGTCTGGCAGCGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACAt                                                                                <  2:1101629/93‑1 (MQ=255)

          gTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATa                                                                               <  2:3133442/93‑1 (MQ=255)

          gTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATa                                                                               <  2:4494992/93‑1 (MQ=255)

           tGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATAc                                                                              <  1:254271/93‑1 (MQ=255)

           ggCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATAc                                                                              <  1:1048060/92‑1 (MQ=255)

              aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGt                                                                           <  2:6135629/93‑1 (MQ=255)

              aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGt                                                                           <  2:5577709/93‑1 (MQ=255)

              aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGt                                                                           >  2:5287539/1‑93 (MQ=255)

               tAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGtt                                                                          >  1:4523022/1‑93 (MQ=255)

               tAAAGGCGTCTGGCAGGGTGCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGtt                                                                          <  2:2350963/93‑1 (MQ=255)

                 aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTga                                                                        >  1:816654/1‑92 (MQ=255)

                   ggCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaa                                                                      >  2:1959197/1‑93 (MQ=255)

                   ggCGTCTGGCAGGGCTCTGTCGAGGCAACGCCAGAAACGTTTTATTCGAACATCGATATCGTCTTGTGTTATAATTCTAACATACTGTTGcaa                                                                      >  1:588436/1‑93 (MQ=255)

                         tGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGc                                                                >  1:683814/1‑93 (MQ=255)

                         tGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGc                                                                <  1:2979014/93‑1 (MQ=255)

                         tGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGc                                                                >  2:2898551/1‑93 (MQ=255)

                              gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa                                                           >  1:993331/1‑93 (MQ=255)

                                 ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGtt                                                        <  1:474704/93‑1 (MQ=255)

                                  tCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTg                                                       >  2:5103112/1‑93 (MQ=255)

                                      tCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGcccc                                                   >  1:3552490/1‑93 (MQ=255)

                                      tCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTATAACATACGGTTGCAACAACGCATCCAGTTGcccc                                                   >  2:279315/1‑93 (MQ=255)

                                       cGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCa                                                  <  1:2484694/93‑1 (MQ=255)

                                        gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAg                                                 <  2:4901389/93‑1 (MQ=255)

                                          ggTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGt                                               >  1:5813037/1‑93 (MQ=255)

                                           gTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGGGTTATAATTCTAACATACGGTTACAACAACGTCTCCAGGTGCCGCAggga                                              >  2:2551386/1‑91 (MQ=255)

                                             aaCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGa                                            <  1:4980778/93‑1 (MQ=255)

                                               cGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAcc                                          <  1:4541490/93‑1 (MQ=255)

                                                gCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCg                                         >  1:745986/1‑93 (MQ=255)

                                                 ccAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCgg                                        >  1:5472391/1‑93 (MQ=255)

                                                   aGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCa                                      >  2:3377308/1‑93 (MQ=255)

                                                    gAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCAt                                     <  1:1968189/93‑1 (MQ=255)

                                                     aaaCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                    >  2:1269757/1‑93 (MQ=255)

                                                     aaaCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                    >  1:895950/1‑93 (MQ=255)

                                                      aaCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCg                                   >  1:1004557/1‑93 (MQ=255)

                                                      aaCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCg                                   >  1:4013535/1‑93 (MQ=255)

                                                       aCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGa                                  <  1:5559651/93‑1 (MQ=255)

                                                         gTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtg                                <  2:4646719/93‑1 (MQ=255)

                                                         gTTTTATGCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtg                                <  1:437858/93‑1 (MQ=255)

                                                          ttttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgt                               >  2:2128494/1‑93 (MQ=255)

                                                          ttttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgt                               >  2:4241923/1‑93 (MQ=255)

                                                          ttttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgt                               >  2:769344/1‑93 (MQ=255)

                                                           tttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgtg                              <  2:2719371/93‑1 (MQ=255)

                                                           tttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgtg                              >  2:2681498/1‑93 (MQ=255)

                                                              aTTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                           <  1:2309976/93‑1 (MQ=255)

                                                              aTTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                           <  1:2012000/93‑1 (MQ=255)

                                                                tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGa                         >  2:3805898/1‑93 (MQ=255)

                                                                 cGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                        <  2:3254455/93‑1 (MQ=255)

                                                                 cGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                        <  1:378354/93‑1 (MQ=255)

                                                                  gAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACg                       >  2:178849/1‑93 (MQ=255)

                                                                  gAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACg                       >  1:2279057/1‑93 (MQ=255)

                                                                     cATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTa                    >  2:4580105/1‑93 (MQ=255)

                                                                     cATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTa                    <  1:1710279/93‑1 (MQ=255)

                                                                      ctCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAc                   <  1:5196639/92‑1 (MQ=255)

                                                                      aTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAc                   <  1:363174/93‑1 (MQ=255)

                                                                       tCGATCTCTTATTGTGGTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAACGGCATCGATGTGACCGACGGCACg                  >  1:3095318/1‑93 (MQ=255)

                                                                       tCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                  >  1:1055428/1‑93 (MQ=255)

                                                                        cGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgt                 <  1:2819409/93‑1 (MQ=255)

                                                                        cGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgt                 <  1:5350871/93‑1 (MQ=255)

                                                                         gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                <  2:4827027/93‑1 (MQ=255)

                                                                         gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                <  1:1193758/93‑1 (MQ=255)

                                                                         gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                >  1:3402882/1‑93 (MQ=255)

                                                                         gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                >  2:3638183/1‑93 (MQ=255)

                                                                         gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGGAGAACGGCATCGCAGTGACCGACGGTAAgtg                >  1:4299873/1‑93 (MQ=255)

                                                                          aTCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg               >  2:5144995/1‑93 (MQ=255)

                                                                          aTCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg               >  1:128213/1‑93 (MQ=255)

                                                                          aTCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg               >  2:347539/1‑93 (MQ=255)

                                                                           tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggt              <  2:73014/93‑1 (MQ=255)

                                                                           tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggt              <  1:3745514/93‑1 (MQ=255)

                                                                           tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggt              >  1:5332251/1‑93 (MQ=255)

                                                                           tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggg              >  2:4192231/1‑92 (MQ=255)

                                                                           tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGGACgtggg              >  2:118139/1‑92 (MQ=255)

                                                                            ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg             >  2:657452/1‑93 (MQ=255)

                                                                            ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg             <  2:2519622/93‑1 (MQ=255)

                                                                             tcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGtggtgg            <  1:2412808/93‑1 (MQ=255)

                                                                              cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTggtggt           <  1:62623/93‑1 (MQ=255)

                                                                              cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTggtggt           >  1:4856090/1‑93 (MQ=255)

                                                                              cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTggtggt           >  1:5317189/1‑93 (MQ=255)

                                                                              cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTggtggt           >  2:1361860/1‑93 (MQ=255)

                                                                               gTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTa          >  2:3505332/1‑93 (MQ=255)

                                                                               gTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTa          >  1:145264/1‑93 (MQ=255)

                                                                               gTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTa          <  2:6012485/93‑1 (MQ=255)

                                                                                tCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaa         >  1:3133576/1‑93 (MQ=255)

                                                                                 cTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaaa        >  2:3918636/1‑93 (MQ=255)

                                                                                  ttGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg       >  2:3996388/1‑93 (MQ=255)

                                                                                  ttGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg       >  2:5650729/1‑93 (MQ=255)

                                                                                  ttGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg       >  2:5983125/1‑93 (MQ=255)

                                                                                  ttGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg       <  2:1613029/93‑1 (MQ=255)

                                                                                   tgtgTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGa      <  2:4137730/93‑1 (MQ=255)

                                                                                   tgtgTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGa      >  2:1999211/1‑93 (MQ=255)

                                                                                      gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATg   <  1:3871030/93‑1 (MQ=255)

                                                                                      gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATg   <  2:2643629/93‑1 (MQ=255)

CGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGG  >  NC_000913/4614148‑4614327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAG  >  NC_000913/4614145‑4614331

GGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAT                                                                                               <  SRR4302532.3115538/94‑1 (MQ=60)

 GCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAACCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATA                                                                                              <  SRR4302532.2379175/94‑1 (MQ=60)

 GCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAAAATCGATCTCGTCTTGTGTTATA                                                                                              >  SRR4302532.5244038/1‑94 (MQ=60)

  CCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAA                                                                                             <  SRR4302532.6215964/94‑1 (MQ=60)

  CCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAA                                                                                             <  SRR4302532.934434/94‑1 (MQ=60)

   CGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAT                                                                                            <  SRR4302532.1021859/94‑1 (MQ=60)

   CGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAT                                                                                            >  SRR4302532.5943520/1‑94 (MQ=60)

   CGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCAAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAT                                                                                            >  SRR4302532.650179/1‑94 (MQ=60)

     ATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTC                                                                                          >  SRR4302532.3740794/1‑94 (MQ=60)

     ATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTC                                                                                          >  SRR4302532.2297060/1‑94 (MQ=60)

       ATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTA                                                                                        >  SRR4302532.4689757/1‑94 (MQ=60)

         TGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAAC                                                                                      <  SRR4302532.2246962/94‑1 (MQ=60)

         TGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAAC                                                                                      >  SRR4302532.3429083/1‑94 (MQ=60)

          GGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACA                                                                                     >  SRR4302532.1823100/1‑94 (MQ=60)

           gcgatcttaaatgcggCTGGCAGGGTTCTGTCGAGGTAACTACAGAAACGTTTTATTCGACCAACGACCTCGTCTTGTGTTATAATTCTAAAAT                                                                                    <  SRR4302532.1063268/78‑1 (MQ=52)

           GCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACAT                                                                                    <  SRR4302532.4643036/94‑1 (MQ=60)

            gcTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATA                                                                                   <  SRR4302532.5514154/92‑1 (MQ=60)

            CGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATA                                                                                   <  SRR4302532.1062266/94‑1 (MQ=60)

            CGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATA                                                                                   <  SRR4302532.4497987/94‑1 (MQ=60)

             GTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATAC                                                                                  <  SRR4302532.810436/94‑1 (MQ=60)

              TGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTTACCTACG                                                                                 >  SRR4302532.444741/1‑94 (MQ=60)

              TGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACG                                                                                 <  SRR4302532.263106/94‑1 (MQ=60)

              TGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACG                                                                                 <  SRR4302532.1083390/94‑1 (MQ=60)

              TGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACG                                                                                 <  SRR4302532.4309800/94‑1 (MQ=60)

              GGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACG                                                                                 <  SRR4302532.1083389/94‑1 (MQ=60)

                 ATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTT                                                                              <  SRR4302532.3549706/94‑1 (MQ=60)

                 ATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTT                                                                              <  SRR4302532.5998947/94‑1 (MQ=60)

                 ATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTT                                                                              >  SRR4302532.4677404/1‑94 (MQ=60)

                   AAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGA                                                                            >  SRR4302532.844507/1‑94 (MQ=60)

                    AAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCA                                                                           <  SRR4302532.2551438/94‑1 (MQ=60)

                     AGGCGTCTGGCAGGGCTCTGTCGAGGCAACGCCAGAAACGTTTTATTCGAACATCGATATCGTCTTGTGTTATAATTCTAACATACTGTTGCAA                                                                          >  SRR4302532.608964/1‑94 (MQ=60)

                      GGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAAC                                                                         <  SRR4302532.2336575/94‑1 (MQ=60)

                         GTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAAC                                                                      <  SRR4302532.5962968/94‑1 (MQ=60)

                         GTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAAC                                                                      >  SRR4302532.1815564/1‑94 (MQ=60)

                          gcgGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACG                                                                     <  SRR4302532.5249297/91‑1 (MQ=60)

                           CTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGC                                                                    >  SRR4302532.707300/1‑94 (MQ=60)

                            TGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCA                                                                   <  SRR4302532.3077562/94‑1 (MQ=60)

                                AGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCA                                                               <  SRR4302532.5745437/94‑1 (MQ=60)

                                AGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCA                                                               >  SRR4302532.3745666/1‑94 (MQ=60)

                                AGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCA                                                               >  SRR4302532.1027217/1‑94 (MQ=60)

                                AGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCA                                                               >  SRR4302532.974935/1‑94 (MQ=60)

                                 GGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAG                                                              <  SRR4302532.6073496/94‑1 (MQ=60)

                                    TTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTG                                                           <  SRR4302532.491247/94‑1 (MQ=60)

                                        GTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCC                                                       >  SRR4302532.3671347/1‑94 (MQ=60)

                                          CGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAG                                                     <  SRR4302532.2567476/94‑1 (MQ=60)

                                            AGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGT                                                   <  SRR4302532.2573789/94‑1 (MQ=60)

                                            AGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGT                                                   >  SRR4302532.6014969/1‑94 (MQ=60)

                                                AACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAC                                               <  SRR4302532.3114172/94‑1 (MQ=60)

                                                AACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAC                                               <  SRR4302532.5151513/94‑1 (MQ=60)

                                                  CGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCG                                             <  SRR4302532.4696514/94‑1 (MQ=60)

                                                  CGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCG                                             >  SRR4302532.771516/1‑94 (MQ=60)

                                                   GCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGG                                            >  SRR4302532.5662122/1‑94 (MQ=60)

                                                   GCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGG                                            >  SRR4302532.2382110/1‑94 (MQ=60)

                                                   GCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGG                                            >  SRR4302532.2647299/1‑94 (MQ=60)

                                                   GCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGG                                            >  SRR4302532.1351468/1‑94 (MQ=60)

                                                     CAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCA                                          <  SRR4302532.5715228/94‑1 (MQ=60)

                                                     CAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCA                                          >  SRR4302532.4697344/1‑94 (MQ=60)

                                                      AGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCAT                                         >  SRR4302532.5529021/1‑94 (MQ=60)

                                                      AGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCAT                                         >  SRR4302532.3066272/1‑94 (MQ=60)

                                                       GAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATC                                        <  SRR4302532.2033573/94‑1 (MQ=60)

                                                       GAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATC                                        >  SRR4302532.926518/1‑94 (MQ=60)

                                                       GAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATC                                        >  SRR4302532.4634405/1‑94 (MQ=60)

                                                        AAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCG                                       >  SRR4302532.1038743/1‑94 (MQ=60)

                                                        AAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCG                                       >  SRR4302532.4148773/1‑94 (MQ=60)

                                                          ACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAT                                     <  SRR4302532.5752689/94‑1 (MQ=60)

                                                           CGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATG                                    >  SRR4302532.1513961/1‑94 (MQ=60)

                                                           CGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATG                                    >  SRR4302532.3232180/1‑94 (MQ=60)

                                                           AGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATG                                    <  SRR4302532.5075834/94‑1 (MQ=60)

                                                            GTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGT                                   >  SRR4302532.3908752/1‑94 (MQ=60)

                                                            GTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGT                                   <  SRR4302532.3454586/94‑1 (MQ=60)

                                                            GTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGT                                   >  SRR4302532.881506/1‑94 (MQ=60)

                                                            GTTTTATGCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGT                                   <  SRR4302532.453019/94‑1 (MQ=60)

                                                              TTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGA                                 >  SRR4302532.631379/1‑94 (MQ=60)

                                                                TATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACC                               <  SRR4302532.2042056/94‑1 (MQ=60)

                                                                 ATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCG                              <  SRR4302532.2386834/94‑1 (MQ=60)

                                                                 ATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCG                              >  SRR4302532.4994930/1‑94 (MQ=60)

                                                                 ATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCG                              <  SRR4302532.2386833/94‑1 (MQ=60)

                                                                 ATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCG                              <  SRR4302532.2079105/94‑1 (MQ=60)

                                                                  TTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGA                             <  SRR4302532.260548/94‑1 (MQ=60)

                                                                   TCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAC                            <  SRR4302532.5610453/94‑1 (MQ=60)

                                                                    CGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACG                           >  SRR4302532.2354699/1‑94 (MQ=60)

                                                                    CGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACG                           <  SRR4302532.391530/94‑1 (MQ=60)

                                                                        CATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAC                       >  SRR4302532.3042762/1‑94 (MQ=60)

                                                                        CATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAC                       <  SRR4302532.1767932/94‑1 (MQ=60)

                                                                         CTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACG                      <  SRR4302532.5375770/94‑1 (MQ=60)

                                                                         ATCGATCTCTTATTGTGGTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAACGGCATCGATGTGACCGACGGCACG                      >  SRR4302532.3197935/1‑94 (MQ=60)

                                                                         ATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACG                      <  SRR4302532.375746/94‑1 (MQ=60)

                                                                         ATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACG                      >  SRR4302532.1090998/1‑94 (MQ=60)

                                                                         ATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACG                      <  SRR4302532.1976233/94‑1 (MQ=60)

                                                                           CGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTG                    >  SRR4302532.3516823/1‑94 (MQ=60)

                                                                           CGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTG                    <  SRR4302532.5536481/94‑1 (MQ=60)

                                                                           CGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTG                    <  SRR4302532.2913769/94‑1 (MQ=60)

                                                                           CGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGGAGAACGGCATCGCAGTGACCGACGGTAAGTG                    >  SRR4302532.4445172/1‑94 (MQ=60)

                                                                            GATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGG                   <  SRR4302532.1233668/94‑1 (MQ=60)

                                                                            GATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGG                   >  SRR4302532.132549/1‑94 (MQ=60)

                                                                            GATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGG                   >  SRR4302532.3796051/1‑94 (MQ=60)

                                                                            GATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGG                   >  SRR4302532.3916080/1‑94 (MQ=60)

                                                                             ATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATTCAGTTGCGCCAGGTAGACAGGCATCGATATGACAATCGGTACGTGGT                  >  SRR4302532.4382575/1‑94 (MQ=60)

                                                                             ATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGcggg                  >  SRR4302532.4041607/1‑90 (MQ=60)

                                                                             ATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGT                  >  SRR4302532.5517159/1‑94 (MQ=60)

                                                                              TCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTG                 >  SRR4302532.6266508/1‑94 (MQ=60)

                                                                              TCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTG                 >  SRR4302532.840293/1‑94 (MQ=60)

                                                                              TCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTG                 <  SRR4302532.3871405/94‑1 (MQ=60)

                                                                              TCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTG                 <  SRR4302532.3871407/94‑1 (MQ=60)

                                                                               CTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGG                >  SRR4302532.4812770/1‑94 (MQ=60)

                                                                                TCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGCCCGACGGTACGTGGTGGT               <  SRR4302532.5153667/94‑1 (MQ=60)

                                                                                TCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGT               <  SRR4302532.2493065/94‑1 (MQ=60)

                                                                                TCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGT               >  SRR4302532.5501173/1‑94 (MQ=60)

                                                                                TCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGT               >  SRR4302532.5023176/1‑94 (MQ=60)

                                                                                 CGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTA              >  SRR4302532.150145/1‑94 (MQ=60)

                                                                                 CGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTA              <  SRR4302532.64877/94‑1 (MQ=60)

                                                                                 CGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGCGGACTGGCATCGATGTGATCGACGGTACGGGGTCGTA              >  SRR4302532.150144/1‑94 (MQ=60)

                                                                                  GTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAA             >  SRR4302532.3237588/1‑94 (MQ=60)

                                                                                  GTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAA             >  SRR4302532.1234841/1‑94 (MQ=60)

                                                                                   TCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAA            >  SRR4302532.5425364/1‑94 (MQ=60)

                                                                                   TCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAA            >  SRR4302532.2205887/1‑94 (MQ=60)

                                                                                    CTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAG           <  SRR4302532.5510895/94‑1 (MQ=60)

                                                                                       GTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAAT        >  SRR4302532.1916129/1‑94 (MQ=60)

                                                                                        TGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATG       >  SRR4302532.2440552/1‑94 (MQ=60)

                                                                                        TGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATG       >  SRR4302532.5867409/1‑94 (MQ=60)

                                                                                         GTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGG      <  SRR4302532.4001658/94‑1 (MQ=60)

                                                                                         GTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGG      >  SRR4302532.1145390/1‑94 (MQ=60)

                                                                                         GTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGG      <  SRR4302532.123175/94‑1 (MQ=60)

                                                                                         GTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGG      >  SRR4302532.749374/1‑94 (MQ=60)

                                                                                          TTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGC     >  SRR4302532.2378422/1‑94 (MQ=60)

                                                                                            ATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCA   >  SRR4302532.3584963/1‑94 (MQ=60)

                                                                                            ATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCA   <  SRR4302532.3189907/94‑1 (MQ=60)

                                                                                            ATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCA   >  SRR4302532.657410/1‑94 (MQ=60)

                                                                                             TAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAG  >  SRR4302532.2315069/1‑94 (MQ=60)

GGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAG  >  NC_000913/4614145‑4614331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`