Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,293,960 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate‑aspartate symport protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,293,9591.C98.9% 351.6 / ‑4.3 176intergenic (+586/+56)gltP/yjcOglutamate‑aspartate symport protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (99/74);  minor base A (2/0);  total (101/75)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,293,9592.G99.4% 357.9 / ‑3.8 176intergenic (+586/+56)gltP/yjcOglutamate‑aspartate symport protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (101/74);  total (101/75)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 189 total aligned reads displayed.

ATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTCA  >  NC_000913/4293924‑4294045
                                      ||                                                                                       
 tttAGCGTGATTTTGGAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTaa                              <  1:1351659/96‑1 (MQ=11)
  ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                             >  2:9589404/1‑96 (MQ=1)
  ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                             >  1:14060047/1‑96 (MQ=1)
  ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                             <  1:13010751/96‑1 (MQ=1)
  ttAGCGTGATTTTGTAGGGCGGATCAGGCGTTT‑‑ACGCCCCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                             <  1:2908886/96‑1 (MQ=1)
  ttAGCGTGATATTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                             >  1:5011410/1‑96 (MQ=1)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGCCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            <  2:4866779/96‑1 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            <  1:865785/96‑1 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            >  2:11008876/1‑96 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            >  2:11526285/1‑96 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            >  1:842841/1‑96 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            <  2:4234121/96‑1 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            <  2:4915923/96‑1 (MQ=11)
   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            >  2:7058854/1‑96 (MQ=11)
   tAGCGTGATTTTGTAGGTCGCATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                            <  2:2169732/96‑1 (MQ=11)
    aGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                           >  2:10948302/1‑96 (MQ=11)
    aGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                           <  2:11784623/96‑1 (MQ=11)
    aGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                           >  2:13931245/1‑96 (MQ=11)
     gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGt                          >  1:5651691/1‑96 (MQ=11)
     gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt                          <  2:799749/96‑1 (MQ=11)
     gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt                          >  1:7874125/1‑96 (MQ=11)
     gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt                          <  1:4081045/96‑1 (MQ=11)
      cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt                         <  1:1143456/96‑1 (MQ=11)
      cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt                         <  2:8512902/96‑1 (MQ=11)
      cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtg                         >  1:6308259/1‑95 (MQ=11)
       gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                        <  2:5794937/96‑1 (MQ=11)
       gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                        >  1:13852911/1‑96 (MQ=11)
       gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                        >  2:4433978/1‑96 (MQ=11)
       gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                        >  2:814770/1‑96 (MQ=11)
       gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                        <  2:14356090/96‑1 (MQ=11)
       gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                        >  2:8039413/1‑96 (MQ=11)
        tGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                       >  2:13620669/1‑96 (MQ=11)
        tGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                       <  1:2037122/96‑1 (MQ=11)
         gATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                      >  1:9422226/1‑96 (MQ=11)
          aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                     <  1:6604116/96‑1 (MQ=14)
          aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                     >  1:12246235/1‑96 (MQ=14)
          aTTTTGTAGGTCGGATAAGGCGTAT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                     <  1:12558990/96‑1 (MQ=14)
           ttttGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑a                   >  2:5755777/1‑96 (MQ=14)
              tGTAGGTCGGATAAGTCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtt                >  2:3453061/1‑96 (MQ=14)
              tGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtt                >  1:1709348/1‑96 (MQ=14)
              tGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtt                <  1:2284232/96‑1 (MQ=14)
              tGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCAGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtt                <  1:62338/96‑1 (MQ=14)
               gTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttt               <  1:6065134/96‑1 (MQ=14)
               gTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttt               >  1:1581410/1‑96 (MQ=14)
               gTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttt               <  1:14347585/96‑1 (MQ=14)
               gTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttt               <  1:11977483/96‑1 (MQ=14)
               gTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttt               >  2:7224375/1‑96 (MQ=14)
                tAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtttt              >  1:3253233/1‑96 (MQ=14)
                tAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtttt              >  2:10617631/1‑96 (MQ=14)
                tAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtttt              >  2:3646552/1‑96 (MQ=14)
                tAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtttt              >  1:7222821/1‑96 (MQ=17)
                tAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtttt              >  2:8679079/1‑96 (MQ=17)
                tAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATACGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAtttt              >  1:12109811/1‑96 (MQ=17)
                 aGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttttt             >  1:9604979/1‑96 (MQ=17)
                 aGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttttt             >  1:5328499/1‑96 (MQ=17)
                 aGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AAttttt             >  1:7022314/1‑96 (MQ=17)
                 aGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGATCTTAACCGTTGGTG‑AAttttt             >  1:10378685/1‑96 (MQ=14)
                  ggTCGGATAAGGCGTTTCCG‑‑CCGCATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTc            <  2:3191024/96‑1 (MQ=17)
                  ggTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTc            <  1:3639179/96‑1 (MQ=17)
                   gTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCa           <  2:4526901/96‑1 (MQ=17)
                   gTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCa           <  2:6651760/96‑1 (MQ=17)
                    tCGNATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCaa          >  2:1116195/1‑96 (MQ=17)
                    tCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCaa          >  2:12125202/1‑96 (MQ=17)
                    tCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCaa          >  2:2754735/1‑96 (MQ=17)
                    gcGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCaa          <  1:6465878/95‑1 (MQ=17)
                     cGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCaaa         >  2:1639490/1‑96 (MQ=17)
                      ggATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGGTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAc        <  1:13971644/96‑1 (MQ=17)
                      ggATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAATTTTTCaaa         <  1:9658425/96‑1 (MQ=14)
                      ggATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAc        >  2:13152940/1‑96 (MQ=17)
                      ggATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAc        >  2:530634/1‑96 (MQ=17)
                      ggATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAc        >  1:1871578/1‑96 (MQ=17)
                      ggATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAc        <  2:6076939/96‑1 (MQ=17)
                       gATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAct       <  2:1278242/96‑1 (MQ=17)
                       gATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAct       <  1:4689249/96‑1 (MQ=17)
                       gATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAAct       <  2:2078624/96‑1 (MQ=17)
                        aTAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActc      >  1:8943347/1‑96 (MQ=18)
                        aTAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActc      >  2:10065012/1‑96 (MQ=18)
                        aTAAGGCGTTT‑‑ACGCCCCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActc      <  1:3978939/96‑1 (MQ=18)
                         tAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATTAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActct     >  2:12223237/1‑96 (MQ=18)
                         tAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCACActct     >  1:9379139/1‑96 (MQ=17)
                         tAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActct     >  1:1535544/1‑96 (MQ=18)
                         tAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActct     <  1:3511468/96‑1 (MQ=18)
                         tAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActct     >  2:10991437/1‑96 (MQ=18)
                         tAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActct     >  1:3825655/1‑96 (MQ=18)
                         tAAGGCGTTT‑‑ACGCCGCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAActct     >  2:12302937/1‑96 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    >  2:5467310/1‑96 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    <  2:9958511/96‑1 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    >  2:9629288/1‑96 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    >  2:9321351/1‑96 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    >  2:9094556/1‑96 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    <  1:11723210/96‑1 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    <  1:5271549/96‑1 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    >  1:2373545/1‑96 (MQ=18)
                          aaGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCtt    <  2:6394359/96‑1 (MQ=18)
                           aGGCGTTT‑‑ACGCCTCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAGCTTAACCGTTGGTT‑AATTTTTCACACTCTTc   >  1:13418066/1‑96 (MQ=17)
                           aGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTc   <  1:14041443/96‑1 (MQ=18)
                           aGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTc   >  1:4766695/1‑96 (MQ=18)
                           aGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTc   >  1:10351872/1‑96 (MQ=18)
                            ggCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTca  <  1:8311707/96‑1 (MQ=18)
                            ggCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTca  >  2:5586900/1‑96 (MQ=18)
                                      ||                                                                                       
ATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑AATTTTTCAAACTCTTCA  >  NC_000913/4293924‑4294045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A