Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,018,363 G→A P305L (CCG→CTG)  ompA ← outer membrane protein 3a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,018,3630GA85.5% 710.4 / 85.3 249P305L (CCG→CTG) ompAouter membrane protein 3a
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base A (122/91);  total (139/110)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

Only 100 of 255 total aligned reads displayed.

CTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCG  >  NC_000913/1018271‑1018455
                                                                                            |                                                                                            
  ttAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCagg                                                                                            <  1:6229252/93‑1 (MQ=255)
  ttAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCagg                                                                                            <  1:1844879/93‑1 (MQ=255)
   tAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGt                                                                                           <  2:3750675/93‑1 (MQ=255)
    aaCTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGtt                                                                                          >  2:2396893/1‑93 (MQ=255)
     aCTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTg                                                                                         >  2:3330897/1‑93 (MQ=255)
       ttCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGa                                                                                       <  1:6536493/93‑1 (MQ=255)
         ggATCTCTACGCGACAATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGAtt                                                                                     <  2:4637052/92‑1 (MQ=255)
         cGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGAtt                                                                                     <  1:3973036/93‑1 (MQ=255)
           aTCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCg                                                                                   >  2:3057144/1‑93 (MQ=255)
           aTCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCg                                                                                   >  1:3029163/1‑93 (MQ=255)
            tctcTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGCGTTGCCAGTAACCAGGTTGGATTCGc                                                                                  >  2:2482602/1‑93 (MQ=255)
              tctACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGccc                                                                                >  2:2329135/1‑93 (MQ=255)
               ctACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCa                                                                               <  1:1935141/93‑1 (MQ=255)
                tACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCAt                                                                              <  2:4718283/93‑1 (MQ=255)
                 aCGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATa                                                                             <  2:5489431/93‑1 (MQ=255)
                    cgacgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCa                                                                          >  2:731114/1‑93 (MQ=255)
                     gacgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCAc                                                                         >  1:4439938/1‑93 (MQ=255)
                     gacgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCAc                                                                         <  1:3673809/93‑1 (MQ=255)
                      acgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACg                                                                        <  2:2199526/93‑1 (MQ=255)
                       cgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGt                                                                       >  2:1681082/1‑93 (MQ=255)
                         aTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGc                                                                     >  2:4541071/1‑93 (MQ=255)
                         aTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGc                                                                     >  1:4107706/1‑93 (MQ=255)
                         aTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGc                                                                     <  1:1534863/93‑1 (MQ=255)
                             ggAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGgag                                                                 <  2:2735389/93‑1 (MQ=255)
                             ggAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGgag                                                                 <  1:5711613/93‑1 (MQ=255)
                             ggAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGgag                                                                 >  1:1495203/1‑93 (MQ=255)
                             ggAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGCTGGATTCGCCCATACCACGTGCGgag                                                                 >  1:1736133/1‑93 (MQ=255)
                              gAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGgaga                                                                >  2:3417264/1‑93 (MQ=255)
                              gAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGgaga                                                                <  1:5440040/93‑1 (MQ=255)
                               aGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGAt                                                               >  1:716570/1‑93 (MQ=255)
                                   aGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTg                                                           >  1:2234311/1‑93 (MQ=255)
                                      cAGTCGATCAGTGCATCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCt                                                        >  2:1010435/1‑93 (MQ=255)
                                       aGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTg                                                       >  2:5220171/1‑93 (MQ=255)
                                       aGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTg                                                       >  2:3035042/1‑93 (MQ=255)
                                       aGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTg                                                       >  1:2090524/1‑93 (MQ=255)
                                        gTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGc                                                      <  1:285275/93‑1 (MQ=255)
                                         tCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGcc                                                     >  2:3242497/1‑93 (MQ=255)
                                          cGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCACACCACGTGCGGAGATCTTGTCTGCCg                                                    >  1:5356205/1‑93 (MQ=255)
                                           gATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGcccg                                                   >  1:3818312/1‑91 (MQ=255)
                                           gATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCgg                                                   <  1:5219288/93‑1 (MQ=255)
                                           gATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCgg                                                   >  1:3387366/1‑93 (MQ=255)
                                            aTCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCggg                                                  >  1:708003/1‑93 (MQ=255)
                                            aTCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCggg                                                  >  1:5015766/1‑93 (MQ=255)
                                            aTCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCggg                                                  >  1:1677756/1‑93 (MQ=255)
                                             tCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGa                                                 >  2:3521918/1‑93 (MQ=255)
                                             tCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGa                                                 >  2:1207405/1‑93 (MQ=255)
                                             tCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGa                                                 <  1:3291672/93‑1 (MQ=255)
                                              cAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGAt                                                >  2:6576295/1‑93 (MQ=255)
                                              cAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGAt                                                <  2:1045591/93‑1 (MQ=255)
                                                  gcagcaCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCt                                            <  2:4132735/93‑1 (MQ=255)
                                                   cagcaCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCtt                                           <  2:1321021/93‑1 (MQ=255)
                                                   cagcaCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCtt                                           <  1:4982486/93‑1 (MQ=255)
                                                    agcaCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGGTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCttt                                          >  2:5810637/1‑93 (MQ=255)
                                                      caCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTgg                                        >  1:3238609/1‑93 (MQ=255)
                                                       aCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGga                                       >  2:5295110/1‑93 (MQ=255)
                                                       aCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGga                                       <  2:3958947/93‑1 (MQ=255)
                                                       aCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGga                                       <  1:6578946/93‑1 (MQ=255)
                                                          cTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGAGATACCTTTGGAGAt                                    >  2:3083627/1‑93 (MQ=255)
                                                            gTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCa                                  >  1:5120112/1‑93 (MQ=255)
                                                            gTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCa                                  <  2:1747862/93‑1 (MQ=255)
                                                            gTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCa                                  >  1:3348048/1‑93 (MQ=255)
                                                               tCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGt                               >  2:1874112/1‑93 (MQ=255)
                                                                cACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTa                              >  1:451103/1‑93 (MQ=255)
                                                                 ccGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTaa                             <  2:6497003/92‑1 (MQ=255)
                                                                 aCGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTaa                             >  2:6532059/1‑93 (MQ=255)
                                                                 aCGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTaa                             >  1:5962776/1‑93 (MQ=255)
                                                                 aCGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTaa                             >  1:4635210/1‑93 (MQ=255)
                                                                  cGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAAt                            <  2:5063270/93‑1 (MQ=255)
                                                                  cGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAAt                            >  2:6056613/1‑93 (MQ=255)
                                                                   gTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTACTc                           >  1:2724807/1‑93 (MQ=255)
                                                                   gTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATc                           >  1:5580893/1‑93 (MQ=255)
                                                                   gTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATc                           <  2:2684255/93‑1 (MQ=255)
                                                                   gTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATc                           <  1:3551737/93‑1 (MQ=255)
                                                                    ttGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATca                          >  2:1528036/1‑93 (MQ=255)
                                                                    ttGTCAAAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATca                          >  1:368756/1‑93 (MQ=255)
                                                                     tGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaa                         <  2:1956093/93‑1 (MQ=255)
                                                                      gTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaac                        <  1:231013/93‑1 (MQ=255)
                                                                      gTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaac                        >  2:6368646/1‑93 (MQ=255)
                                                                      gTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaac                        >  1:479001/1‑93 (MQ=255)
                                                                       tCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaaca                       <  2:3758791/93‑1 (MQ=255)
                                                                        cacaGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaacaa                      <  1:6083201/93‑1 (MQ=255)
                                                                        cacaGGCGTTCCCAGTAACCAGGTTAGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaacaa                      <  2:6187849/93‑1 (MQ=255)
                                                                          caGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAacaaca                    >  1:1050363/1‑93 (MQ=255)
                                                                          caGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAacaaca                    <  2:4314239/93‑1 (MQ=255)
                                                                           aGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAg                   >  1:2912862/1‑93 (MQ=255)
                                                                            ggTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGa                  >  2:6611787/1‑93 (MQ=255)
                                                                             gtgtTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGAc                 <  2:6009143/93‑1 (MQ=255)
                                                                             gtgtTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGAc                 >  2:4794558/1‑93 (MQ=255)
                                                                               gtTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTg               >  1:5556459/1‑93 (MQ=255)
                                                                                ttGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGa              <  1:5909848/93‑1 (MQ=255)
                                                                                ttGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGa              >  1:1307325/1‑93 (MQ=255)
                                                                                  gCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGc            >  1:786002/1‑93 (MQ=255)
                                                                                    cAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCAc          >  2:5629212/1‑93 (MQ=255)
                                                                                     aGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACg         >  2:2569022/1‑93 (MQ=255)
                                                                                     aGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACg         >  2:2037419/1‑93 (MQ=255)
                                                                                      gTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg        <  2:5288181/93‑1 (MQ=255)
                                                                                       tAACCAGGTTGGATTCGCCAATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTCGGAGATCAGGTAATCAACAACAGACTGAGCACGgc       <  2:4290009/93‑1 (MQ=255)
                                                                                         aCCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGgcgc     >  2:4213606/1‑93 (MQ=255)
                                                                                          ccGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCt    <  1:3810283/93‑1 (MQ=255)
                                                                                            gggTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCg  >  1:4355701/1‑93 (MQ=255)
                                                                                            |                                                                                            
CTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCG  >  NC_000913/1018271‑1018455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGG  >  NC_000913/1018270‑1018456
                                                                                             |                                                                                             
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCA                                                                                               >  SRR4302547.343149/1‑94 (MQ=60)
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCA                                                                                               <  SRR4302547.2656974/94‑1 (MQ=60)
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCA                                                                                               >  SRR4302547.2839496/1‑94 (MQ=60)
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCA                                                                                               <  SRR4302547.1637909/94‑1 (MQ=60)
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCA                                                                                               >  SRR4302547.3922797/1‑94 (MQ=60)
 CTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAG                                                                                              >  SRR4302547.2141325/1‑94 (MQ=60)
  TTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGG                                                                                             <  SRR4302547.5446528/94‑1 (MQ=60)
   TTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGT                                                                                            <  SRR4302547.6358632/94‑1 (MQ=60)
   TTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGT                                                                                            <  SRR4302547.1881331/94‑1 (MQ=60)
     AACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTG                                                                                          <  SRR4302547.2222925/94‑1 (MQ=60)
     AACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTG                                                                                          <  SRR4302547.6165958/94‑1 (MQ=60)
       CTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGA                                                                                        >  SRR4302547.5008074/1‑94 (MQ=60)
        TTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGAT                                                                                       <  SRR4302547.6672514/94‑1 (MQ=60)
         TCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATT                                                                                      <  SRR4302547.232495/94‑1 (MQ=60)
          CGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTC                                                                                     <  SRR4302547.4053831/94‑1 (MQ=60)
           GATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCG                                                                                    >  SRR4302547.5095072/1‑94 (MQ=60)
           GATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCG                                                                                    >  SRR4302547.3089397/1‑94 (MQ=60)
            ATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGC                                                                                   >  SRR4302547.5241753/1‑94 (MQ=60)
              CTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCC                                                                                 <  SRR4302547.1771142/94‑1 (MQ=60)
              CTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGCGTTGCCAGTAACCAGGTTGGATTCGCCC                                                                                 >  SRR4302547.4005961/1‑94 (MQ=60)
                CTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCAT                                                                               <  SRR4302547.749118/94‑1 (MQ=60)
                CTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCAT                                                                               <  SRR4302547.1973523/94‑1 (MQ=60)
                  ACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATAC                                                                             >  SRR4302547.810571/1‑94 (MQ=60)
                   CGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACC                                                                            >  SRR4302547.5897041/1‑94 (MQ=60)
                     CGACGATCGGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCAC                                                                          >  SRR4302547.5479770/1‑94 (MQ=60)
                     CGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCAC                                                                          >  SRR4302547.4531743/1‑94 (MQ=60)
                     CGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCAC                                                                          <  SRR4302547.4732312/94‑1 (MQ=60)
                      GACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACG                                                                         >  SRR4302547.4329514/1‑94 (MQ=60)
                      GACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACG                                                                         >  SRR4302547.3868127/1‑94 (MQ=60)
                      GACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACG                                                                         <  SRR4302547.3748752/94‑1 (MQ=60)
                         GATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGC                                                                      >  SRR4302547.4191195/1‑94 (MQ=60)
                          ATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCG                                                                     <  SRR4302547.1012424/94‑1 (MQ=60)
                          ATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCG                                                                     <  SRR4302547.1565412/94‑1 (MQ=60)
                            CCGGAGCCAGGCAGTCGATCAGTGCACCCCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACAACGTGCGGA                                                                   <  SRR4302547.2953410/94‑1 (MQ=60)
                             CGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAG                                                                  <  SRR4302547.2485893/94‑1 (MQ=60)
                             CGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAG                                                                  >  SRR4302547.3430172/1‑94 (MQ=60)
                             CGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAG                                                                  >  SRR4302547.4466961/1‑94 (MQ=60)
                             CGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAG                                                                  >  SRR4302547.1525031/1‑94 (MQ=60)
                             CGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGCTGGATTCGCCCATACCACGTGCGGAG                                                                  >  SRR4302547.1770248/1‑94 (MQ=60)
                              GGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGA                                                                 <  SRR4302547.5829578/94‑1 (MQ=60)
                               GAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGAT                                                                >  SRR4302547.5636966/1‑94 (MQ=60)
                               GAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGAT                                                                <  SRR4302547.5552463/94‑1 (MQ=60)
                               GAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGAT                                                                >  SRR4302547.729848/1‑94 (MQ=60)
                                AGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATC                                                               >  SRR4302547.3651515/1‑94 (MQ=60)
                                 GCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCT                                                              <  SRR4302547.6046112/94‑1 (MQ=60)
                                 GCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCT                                                              <  SRR4302547.3462371/94‑1 (MQ=60)
                                  CCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATGCCACGTGCGGAGATCTT                                                             <  SRR4302547.4547415/94‑1 (MQ=60)
                                  CCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGCTCTT                                                             >  SRR4302547.3371637/1‑94 (MQ=60)
                                  CCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTT                                                             <  SRR4302547.2068215/94‑1 (MQ=60)
                                   CAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTG                                                            >  SRR4302547.2279352/1‑94 (MQ=60)
                                    AGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGT                                                           >  SRR4302547.726344/1‑94 (MQ=60)
                                     GGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTC                                                          <  SRR4302547.256070/94‑1 (MQ=60)
                                      GCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCT                                                         <  SRR4302547.1163327/94‑1 (MQ=60)
                                       CAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTG                                                        >  SRR4302547.282437/1‑94 (MQ=60)
                                       CAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTG                                                        >  SRR4302547.2132195/1‑94 (MQ=60)
                                         GTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCC                                                      <  SRR4302547.3978353/94‑1 (MQ=60)
                                         GTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCC                                                      <  SRR4302547.290583/94‑1 (MQ=60)
                                         GTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCC                                                      <  SRR4302547.1697080/94‑1 (MQ=60)
                                          TCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCACACCACGTGCGGAGATCTTGTCTGCCG                                                     >  SRR4302547.5466677/1‑94 (MQ=60)
                                           CGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGG                                                    >  SRR4302547.3456264/1‑94 (MQ=60)
                                           CGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGG                                                    >  SRR4302547.4323337/1‑94 (MQ=60)
                                           CGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCCG                                                    >  SRR4302547.3896115/1‑94 (MQ=60)
                                            GATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGG                                                   >  SRR4302547.721095/1‑94 (MQ=60)
                                            GATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGG                                                   <  SRR4302547.5326709/94‑1 (MQ=60)
                                            GATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGG                                                   >  SRR4302547.1710804/1‑94 (MQ=60)
                                            GATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGG                                                   >  SRR4302547.5119440/1‑94 (MQ=60)
                                             ATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGA                                                  >  SRR4302547.3090946/1‑94 (MQ=60)
                                              TCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGAT                                                 <  SRR4302547.5707028/94‑1 (MQ=60)
                                              TCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGAT                                                 <  SRR4302547.3357771/94‑1 (MQ=60)
                                               CAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATA                                                >  SRR4302547.6734076/1‑94 (MQ=60)
                                                   GCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTT                                            <  SRR4302547.5678070/94‑1 (MQ=60)
                                                    CAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTT                                           >  SRR4302547.381832/1‑94 (MQ=60)
                                                    CAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTT                                           <  SRR4302547.5085528/94‑1 (MQ=60)
                                                    CAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTT                                           <  SRR4302547.6557527/94‑1 (MQ=60)
                                                      GCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGG                                         >  SRR4302547.3303257/1‑94 (MQ=60)
                                                      GCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGG                                         <  SRR4302547.6759295/94‑1 (MQ=60)
                                                      GCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGG                                         <  SRR4302547.1462532/94‑1 (MQ=60)
                                                      GCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGG                                         <  SRR4302547.4587924/94‑1 (MQ=60)
                                                        ACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATAGCTTTGGAG                                       >  SRR4302547.1370151/1‑94 (MQ=60)
                                                        ACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAG                                       >  SRR4302547.2297755/1‑94 (MQ=60)
                                                        ACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAG                                       <  SRR4302547.6715939/94‑1 (MQ=60)
                                                          GCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCTGAGATCTTGTCTGCCGGGATACCTTTTGAGAT                                     >  SRR4302547.1828935/1‑94 (MQ=60)
                                                            TGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCA                                   >  SRR4302547.5225597/1‑94 (MQ=60)
                                                            TGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCA                                   >  SRR4302547.3415917/1‑94 (MQ=60)
                                                            TGTTTCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCA                                   >  SRR4302547.607797/1‑94 (MQ=60)
                                                                TCACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTA                               >  SRR4302547.459472/1‑94 (MQ=60)
                                                                 gacggTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAA                              >  SRR4302547.3752323/6‑94 (MQ=60)
                                                                 CACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAA                              >  SRR4302547.1722452/1‑94 (MQ=60)
                                                                 CACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAA                              >  SRR4302547.6086434/1‑94 (MQ=60)
                                                                 CACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAA                              >  SRR4302547.4731223/1‑94 (MQ=60)
                                                                 CACGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAA                              >  SRR4302547.757292/1‑94 (MQ=60)
                                                                   CGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTACTC                            >  SRR4302547.2779511/1‑94 (MQ=60)
                                                                   CGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATC                            >  SRR4302547.5696010/1‑94 (MQ=60)
                                                                   CGTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATC                            >  SRR4302547.2001742/1‑94 (MQ=60)
                                                                    GTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCA                           <  SRR4302547.3624224/94‑1 (MQ=60)
                                                                    GTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCA                           >  SRR4302547.4158411/1‑94 (MQ=60)
                                                                    GTTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCA                           <  SRR4302547.5366045/94‑1 (MQ=60)
                                                                    GTTGTCAAAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCA                           >  SRR4302547.375590/1‑94 (MQ=60)
                                                                     TTGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAA                          >  SRR4302547.5200355/1‑94 (MQ=60)
                                                                      TGTCACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAAC                         >  SRR4302547.487792/1‑94 (MQ=60)
                                                                       GTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACA                        <  SRR4302547.235360/94‑1 (MQ=60)
                                                                        TCACGGGTTTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAA                       <  SRR4302547.2948094/94‑1 (MQ=60)
                                                                         CACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAAC                      >  SRR4302547.6421389/1‑94 (MQ=60)
                                                                         CACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAAC                      >  SRR4302547.1536922/1‑94 (MQ=60)
                                                                         CACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAAC                      <  SRR4302547.6209404/94‑1 (MQ=60)
                                                                          ACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACA                     >  SRR4302547.4955223/1‑94 (MQ=60)
                                                                          ACAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACA                     >  SRR4302547.1071183/1‑94 (MQ=60)
                                                                           CAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAG                    <  SRR4302547.6273204/94‑1 (MQ=60)
                                                                           CAGGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAG                    >  SRR4302547.2970969/1‑94 (MQ=60)
                                                                             GGTGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGAC                  <  SRR4302547.4670961/94‑1 (MQ=60)
                                                                               TGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATCCCTTTGGAGATCAGGTAATCAACAACAGACTG                <  SRR4302547.3316856/94‑1 (MQ=60)
                                                                               TGTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTG                >  SRR4302547.5671078/1‑94 (MQ=60)
                                                                                GTTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGA               >  SRR4302547.1333615/1‑94 (MQ=60)
                                                                                 TTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAG              <  SRR4302547.6032233/94‑1 (MQ=60)
                                                                                 TTGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAG              >  SRR4302547.1607423/1‑94 (MQ=60)
                                                                                  TGCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGC             >  SRR4302547.800877/1‑94 (MQ=60)
                                                                                   GCCAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCA            <  SRR4302547.338057/94‑1 (MQ=60)
                                                                                    CCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCAC           <  SRR4302547.668530/94‑1 (MQ=60)
                                                                                     CAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACG          >  SRR4302547.3731239/1‑94 (MQ=60)
                                                                                     CAGTAACCAGGTTGGATTCGCCCATCCCACGTGCGGAGATCGTGTCTGCCGGGATCCCTTTGGAGATCAGGTAATAAACAACAGACTGAGCACG          <  SRR4302547.5554579/94‑1 (MQ=60)
                                                                                     CAGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACG          >  SRR4302547.3612952/1‑94 (MQ=60)
                                                                                      AGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGG         >  SRR4302547.3012584/1‑94 (MQ=60)
                                                                                      AGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGG         <  SRR4302547.3233495/94‑1 (MQ=60)
                                                                                      AGTAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGG         >  SRR4302547.3895630/1‑94 (MQ=60)
                                                                                        TAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCG       <  SRR4302547.4739615/94‑1 (MQ=60)
                                                                                        TAACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCG       >  SRR4302547.53678/1‑94 (MQ=60)
                                                                                         AACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGC      >  SRR4302547.5874856/1‑94 (MQ=60)
                                                                                         AACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGC      >  SRR4302547.1903490/1‑94 (MQ=60)
                                                                                         AACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGC      >  SRR4302547.5700793/1‑94 (MQ=60)
                                                                                          ACCAGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCT     <  SRR4302547.520450/94‑1 (MQ=60)
                                                                                           CCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTC    <  SRR4302547.3887938/94‑1 (MQ=60)
                                                                                            CGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCG   >  SRR4302547.4445173/1‑94 (MQ=60)
                                                                                             AGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAatgtcaagatcggaagagcgtcgtgtagggaaagagtg  >  SRR4302547.4215531/1‑56 (MQ=60)
                                                                                             AGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGG  >  SRR4302547.1588624/1‑94 (MQ=60)
                                                                                             AGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGG  >  SRR4302547.6653744/1‑94 (MQ=60)
                                                                                             AGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGG  <  SRR4302547.2913478/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGG  >  NC_000913/1018270‑1018456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑