Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,073,812 T→C C116C (TGT→TGC)  ycdC → putative tet operon regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,073,8120TC85.8% 575.3 / 77.9 219C116C (TGT→TGC) ycdCputative tet operon regulator
Reads supporting (aligned to +/- strand):  ref base T (21/10);  new base C (103/85);  total (124/95)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 229 total aligned reads displayed.

GATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGCC  >  NC_000913/1073723‑1073902
                                                                                         |                                                                                          
gATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATg                                                                                         <  2:5309717/93‑1 (MQ=255)
gATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATg                                                                                         <  1:4887299/93‑1 (MQ=255)
  tttCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGga                                                                                       >  1:357939/1‑93 (MQ=255)
  tttCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGga                                                                                       <  1:237288/93‑1 (MQ=255)
  tttCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGGTCTGTATGga                                                                                       >  1:938742/1‑93 (MQ=255)
  tttCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGAGCCTGTTCTGCATGga                                                                                       >  2:5897470/1‑93 (MQ=255)
    tCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGgaga                                                                                     <  2:3051048/93‑1 (MQ=255)
    tCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGgaga                                                                                     <  1:3745904/93‑1 (MQ=255)
    tCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGgaga                                                                                     <  2:1864144/93‑1 (MQ=255)
     cGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGAt                                                                                    <  2:3008191/93‑1 (MQ=255)
      gCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATg                                                                                   >  2:886651/1‑93 (MQ=255)
      gCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATg                                                                                   >  1:2658471/1‑93 (MQ=255)
      gCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATg                                                                                   >  2:2445985/1‑93 (MQ=255)
        cccGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCt                                                                                 >  2:2650211/1‑93 (MQ=255)
        cccGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCt                                                                                 >  1:6398710/1‑93 (MQ=255)
        cccGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCt                                                                                 >  2:277467/1‑93 (MQ=255)
         ccGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCTCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTg                                                                                <  2:4350678/93‑1 (MQ=255)
         ccGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTg                                                                                >  1:5826534/1‑93 (MQ=255)
            cTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCa                                                                             >  2:6600431/1‑93 (MQ=255)
            cTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCa                                                                             >  2:685/1‑93 (MQ=255)
              ggcggcGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAgg                                                                           <  2:207398/93‑1 (MQ=255)
               gcggcgATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGgc                                                                          >  1:4079735/1‑93 (MQ=255)
                cggcgATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGgcg                                                                         >  1:6185881/1‑93 (MQ=255)
                cggcgATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGgcg                                                                         >  2:2005488/1‑93 (MQ=255)
                  gcgATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGgcgcg                                                                       >  2:5004422/1‑93 (MQ=255)
                    gATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGgcgcgac                                                                     >  2:3210188/1‑91 (MQ=255)
                    gATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGcgcc                                                                     >  1:1698550/1‑93 (MQ=255)
                     aTCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccg                                                                    >  2:3599241/1‑93 (MQ=255)
                      tCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGcgccgc                                                                   <  2:1752729/93‑1 (MQ=255)
                      tCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGcgccgc                                                                   <  2:6292872/93‑1 (MQ=255)
                      tCAAAGAGAACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGcgccgc                                                                   >  1:4484563/1‑93 (MQ=255)
                       cAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCt                                                                  >  1:3445753/1‑93 (MQ=255)
                        aaaGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTg                                                                 >  1:2245265/1‑93 (MQ=255)
                         aaGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGt                                                                >  1:5512596/1‑93 (MQ=255)
                         aaGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGt                                                                >  2:6627327/1‑93 (MQ=255)
                         aaGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGt                                                                >  2:2831564/1‑93 (MQ=255)
                             gTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAAt                                                            >  1:6209003/1‑93 (MQ=255)
                              tACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATg                                                           >  2:1271013/1‑93 (MQ=255)
                              tACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATg                                                           <  2:2884368/93‑1 (MQ=255)
                               aCATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATgg                                                          <  2:3449415/93‑1 (MQ=255)
                                cATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATggg                                                         >  1:4528201/1‑92 (MQ=255)
                                cATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGa                                                         <  2:6494934/93‑1 (MQ=255)
                                 aTCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGAt                                                        <  2:6469182/93‑1 (MQ=255)
                                 aTCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGACTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGAt                                                        >  2:2100148/1‑93 (MQ=255)
                                     gTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAAc                                                    >  1:308913/1‑93 (MQ=255)
                                        tGAAGCTGGAAGTCTCACGCGATTATCCGCGGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGa                                                 <  1:399906/93‑1 (MQ=255)
                                        tGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGa                                                 >  2:529572/1‑93 (MQ=255)
                                        tGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGGCGCTGTTAATGGATGAACTGa                                                 >  1:5665913/1‑93 (MQ=255)
                                        tGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGGACTGa                                                 >  1:2745881/1‑93 (MQ=255)
                                        tGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGa                                                 >  1:1051000/1‑93 (MQ=255)
                                         gAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGAc                                                >  2:3688978/1‑93 (MQ=255)
                                          aaGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACg                                               >  1:759676/1‑93 (MQ=255)
                                          aaGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACg                                               <  1:1992792/93‑1 (MQ=255)
                                           aGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACgg                                              >  1:5334900/1‑93 (MQ=255)
                                            gCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACggg                                             >  2:1899041/1‑93 (MQ=255)
                                             cTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGc                                            >  2:3641481/1‑93 (MQ=255)
                                              tGGAGGTCTCGCGCGATTATCCGCAGGCTGCGCGCCTGTTCTGCAGGGAGATGCGGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCg                                           <  2:5623092/93‑1 (MQ=255)
                                              tGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCg                                           <  2:4261008/93‑1 (MQ=255)
                                               ggAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGa                                          >  1:5935005/1‑93 (MQ=255)
                                                 aaGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGAtt                                        <  2:800077/93‑1 (MQ=255)
                                                 aaGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGAtt                                        <  2:3300543/93‑1 (MQ=255)
                                                    tctcACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGa                                     >  2:2568717/1‑93 (MQ=255)
                                                    tctcACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGa                                     <  1:5175713/93‑1 (MQ=255)
                                                     ctcACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGaa                                    <  1:2133406/93‑1 (MQ=255)
                                                      tcACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAg                                   >  1:4151810/1‑93 (MQ=255)
                                                      tcACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAg                                   >  1:328576/1‑93 (MQ=255)
                                                      tcACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCCCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAg                                   <  1:5026381/93‑1 (MQ=255)
                                                        aCGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGc                                 <  2:1055901/93‑1 (MQ=255)
                                                        aCGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGc                                 >  1:6615528/1‑93 (MQ=255)
                                                        aCGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAggg                                 >  1:3115739/1‑92 (MQ=255)
                                                        aCGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGc                                 >  2:3865761/1‑93 (MQ=255)
                                                         cgcgATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCa                                <  1:1746302/93‑1 (MQ=255)
                                                           cgATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCCCTGTTAATGGATGAACTGACGGGCGGTTTGAAGGCAtt                              >  2:1186105/1‑93 (MQ=255)
                                                             aTTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTaa                            <  2:6223830/93‑1 (MQ=255)
                                                             aTTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTaa                            <  1:3004415/93‑1 (MQ=255)
                                                                   cGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGGTTTGAAGGCATTAATtgatg                      >  1:3616968/1‑93 (MQ=255)
                                                                    gCAGTCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATtgatga                     <  1:5771397/93‑1 (MQ=255)
                                                                    gCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATtgatga                     >  2:3024197/1‑93 (MQ=255)
                                                                      aGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATgaga                   >  1:1481552/1‑93 (MQ=255)
                                                                        gCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGaaa                 <  2:5253734/93‑1 (MQ=255)
                                                                        gCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGaaa                 >  1:2816784/1‑93 (MQ=255)
                                                                        gCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGaaa                 <  1:4386008/93‑1 (MQ=255)
                                                                        gCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGaaa                 <  2:5836280/93‑1 (MQ=255)
                                                                         cTTCGCGCGTGTCCTGCATGGAGATGCTGGCATGCGCGCCGCTGTTAATGGATGACCTGACGGGCGATTTGAAGGCATTAATTGATGAGAAAt                <  1:1886155/93‑1 (MQ=255)
                                                                         cTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACTGGCGATTTGAAGGCATTAATTGATGAGAAAt                <  1:5406901/93‑1 (MQ=255)
                                                                         cTTCGCCCCTGTTCTGCAGGGAGATGCTGGCAGGCGCACCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAAt                <  1:2434045/93‑1 (MQ=255)
                                                                             gcgcCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgc            <  2:31493/93‑1 (MQ=255)
                                                                             gcgcCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgc            >  1:5604813/1‑93 (MQ=255)
                                                                               gcgtGTTCTGCAGGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgcgc          <  2:448411/90‑1 (MQ=255)
                                                                               gcCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgcgc          >  2:5348102/1‑93 (MQ=255)
                                                                               gcCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgcgc          <  2:3761407/93‑1 (MQ=255)
                                                                               gcCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgcgc          >  2:4464760/1‑93 (MQ=255)
                                                                               gcCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgcgc          <  2:5400450/93‑1 (MQ=255)
                                                                               gcCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGgcgc          <  2:5083734/93‑1 (MQ=255)
                                                                                ccTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCt         <  2:1548083/93‑1 (MQ=255)
                                                                                 cTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTg        >  2:4113960/1‑93 (MQ=255)
                                                                                    ttCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGAtt     <  1:4573668/93‑1 (MQ=255)
                                                                                    ttCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGAtt     <  2:396714/93‑1 (MQ=255)
                                                                                      cTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGc   >  2:1689925/1‑93 (MQ=255)
                                                                                       tGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGcc  >  2:912432/1‑93 (MQ=255)
                                                                                         |                                                                                          
GATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGCC  >  NC_000913/1073723‑1073902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGCCG  >  NC_000913/1073720‑1073903
                                                                                            |                                                                                           
GAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCA                                                                                            >  SRR4302547.4383149/1‑94 (MQ=60)
GAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCA                                                                                            >  SRR4302547.2631030/1‑94 (MQ=60)
  AGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATG                                                                                          >  SRR4302547.1811040/1‑94 (MQ=60)
   GATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGG                                                                                         <  SRR4302547.4988539/94‑1 (MQ=60)
    ATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGA                                                                                        >  SRR4302547.364579/1‑94 (MQ=60)
    ATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGA                                                                                        <  SRR4302547.895778/94‑1 (MQ=60)
    ATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGGTCTGTATGGA                                                                                        >  SRR4302547.956921/1‑94 (MQ=60)
     TTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAG                                                                                       <  SRR4302547.241739/94‑1 (MQ=60)
      TTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGA                                                                                      >  SRR4302547.6395307/1‑94 (MQ=60)
       TCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGAT                                                                                     <  SRR4302547.3822369/94‑1 (MQ=60)
        CGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATG                                                                                    >  SRR4302547.2711893/1‑94 (MQ=60)
         GCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGT                                                                                   >  SRR4302547.3342449/1‑94 (MQ=60)
          CCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGGCTGTTCTGCATGGAGCTGCT                                                                                  >  SRR4302547.5481208/1‑94 (MQ=60)
          CCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCT                                                                                  >  SRR4302547.6532185/1‑94 (MQ=60)
           CCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTG                                                                                 >  SRR4302547.6558920/1‑94 (MQ=60)
           CCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTG                                                                                 >  SRR4302547.5947120/1‑94 (MQ=60)
             CGCTGGCGGCGATCAAAGGGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTCCATGGAGATGCGGGC                                                                               >  SRR4302547.5422198/1‑94 (MQ=60)
                TGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGG                                                                            <  SRR4302547.4295077/94‑1 (MQ=60)
                 GGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGC                                                                           >  SRR4302547.4162643/1‑94 (MQ=60)
                 GGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGC                                                                           <  SRR4302547.6251564/94‑1 (MQ=60)
                  GCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCG                                                                          >  SRR4302547.6314272/1‑94 (MQ=60)
                    GGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCTCCTGTTCTGCATGGAGATGCTGGCAGGCGCG                                                                        <  SRR4302547.2761287/94‑1 (MQ=60)
                      CGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCC                                                                      >  SRR4302547.1731942/1‑94 (MQ=60)
                       GATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCG                                                                     >  SRR4302547.5110015/1‑94 (MQ=60)
                       GATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCG                                                                     <  SRR4302547.5882154/94‑1 (MQ=60)
                        ATCAAAGAGAACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGC                                                                    >  SRR4302547.4577212/1‑94 (MQ=60)
                         TNAAAGAGTACATCCNTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTNNNNNNNGNGATGCTGGCNNNNNCGCCNCT                                                                   >  SRR4302547.6054996/1‑94 (MQ=60)
                         TCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCT                                                                   >  SRR4302547.3516002/1‑94 (MQ=60)
                          CAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTG                                                                  >  SRR4302547.2290562/1‑94 (MQ=60)
                           AAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGT                                                                 >  SRR4302547.5626396/1‑94 (MQ=60)
                           AAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGT                                                                 >  SRR4302547.67296/1‑94 (MQ=60)
                           AAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGT                                                                 >  SRR4302547.3663337/1‑94 (MQ=60)
                            AANAGTANNTNCGTCTGAAGCTGGNANNNNCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTT                                                                <  SRR4302547.4251508/94‑1 (MQ=60)
                            AAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTT                                                                >  SRR4302547.4631193/1‑94 (MQ=60)
                            AAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTT                                                                >  SRR4302547.4063056/1‑94 (MQ=60)
                             AGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTA                                                               <  SRR4302547.683210/94‑1 (MQ=60)
                             AGAGCACATCCGTCTGAAGCTGGAAGTCTCAGGCGATTATCCGCAGGCTTCGTGTCTGTTCTGCATGGAGGTGCTGGCAGGTGGGCGGCTGTTA                                                               >  SRR4302547.1231516/1‑94 (MQ=57)
                               AGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAAT                                                             >  SRR4302547.6337936/1‑94 (MQ=60)
                                 TACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGG                                                           <  SRR4302547.4751628/94‑1 (MQ=60)
                                  ACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGG                                                          >  SRR4302547.4621782/1‑94 (MQ=60)
                                   CATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGAT                                                         <  SRR4302547.3464379/94‑1 (MQ=60)
                                       CGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAAC                                                     >  SRR4302547.314660/1‑94 (MQ=60)
                                        GTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATTGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACT                                                    <  SRR4302547.4484641/94‑1 (MQ=60)
                                        GTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACT                                                    >  SRR4302547.3568040/1‑94 (MQ=60)
                                          CTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGA                                                  >  SRR4302547.5978389/1‑94 (MQ=60)
                                          CTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGGCGCTGTTAATGGATGAACTGA                                                  >  SRR4302547.5782903/1‑94 (MQ=60)
                                          CTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGTACTGT                                                  >  SRR4302547.5171110/1‑94 (MQ=60)
                                          CTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGGACTGA                                                  >  SRR4302547.2800949/1‑94 (MQ=60)
                                          CTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGA                                                  >  SRR4302547.1071842/1‑94 (MQ=60)
                                           TGAAGCTGGAAGTCTCACGCGATTATCCGCGGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGAC                                                 <  SRR4302547.407345/94‑1 (MQ=60)
                                           TGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGAC                                                 <  SRR4302547.5650234/94‑1 (MQ=60)
                                            GAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACG                                                >  SRR4302547.773997/1‑94 (MQ=60)
                                             AAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGG                                               >  SRR4302547.417820/1‑94 (MQ=60)
                                             AAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGG                                               >  SRR4302547.5444829/1‑94 (MQ=60)
                                             AAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGG                                               <  SRR4302547.2032420/94‑1 (MQ=60)
                                              AGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGG                                              <  SRR4302547.2476023/94‑1 (MQ=60)
                                               GCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTTATGGATGAACTGACGGGC                                             >  SRR4302547.5933726/1‑94 (MQ=60)
                                                CTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCG                                            >  SRR4302547.198329/1‑94 (MQ=60)
                                                CTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCG                                            <  SRR4302547.2148815/94‑1 (MQ=60)
                                                 TGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGA                                           <  SRR4302547.4041333/94‑1 (MQ=60)
                                                 TGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGA                                           >  SRR4302547.6058067/1‑94 (MQ=60)
                                                 TGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGA                                           >  SRR4302547.486019/1‑94 (MQ=60)
                                                 TGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATAGATGAACTGACGGGCGA                                           >  SRR4302547.995145/1‑94 (MQ=60)
                                                       TCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAA                                     <  SRR4302547.5282259/94‑1 (MQ=60)
                                                        CTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAG                                    >  SRR4302547.334691/1‑94 (MQ=60)
                                                        CTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAG                                    <  SRR4302547.2175903/94‑1 (MQ=60)
                                                        CTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAG                                    >  SRR4302547.4476672/1‑94 (MQ=60)
                                                        CTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAG                                    >  SRR4302547.4236376/1‑94 (MQ=60)
                                                        CTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAG                                    <  SRR4302547.2671319/94‑1 (MQ=60)
                                                         TCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGG                                   <  SRR4302547.2745866/94‑1 (MQ=60)
                                                         TCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCCCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGG                                   <  SRR4302547.5130233/94‑1 (MQ=60)
                                                          CACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGC                                  >  SRR4302547.6753230/1‑94 (MQ=60)
                                                          CACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGG                                  >  SRR4302547.3177499/1‑94 (MQ=60)
                                                            CGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCAT                                >  SRR4302547.1757701/1‑94 (MQ=60)
                                                            CGCGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCAT                                <  SRR4302547.1780742/94‑1 (MQ=60)
                                                              CGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTA                              >  SRR4302547.5489004/1‑94 (MQ=60)
                                                              CGATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTA                              <  SRR4302547.4602313/94‑1 (MQ=60)
                                                               GATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAA                             >  SRR4302547.2216706/1‑94 (MQ=60)
                                                                attagctgcggtattcgcgctgaTTCTGCATGGAGATGCTGTCAGGCGCGCCGCTGTTAATGGATGATCTGACAGGCGATTTGAAGGTATTAAT                            <  SRR4302547.3064178/71‑1 (MQ=60)
                                                                ATTATCCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAAT                            <  SRR4302547.3064179/94‑1 (MQ=60)
                                                                     CCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGGTTTGAAGGCATTAATTGATG                       >  SRR4302547.3690758/1‑94 (MQ=60)
                                                                     CCGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATG                       >  SRR4302547.6331863/1‑94 (MQ=60)
                                                                      CGCAGGCTTCGCGCCTGTTCTGTATGGAGAGGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGA                      <  SRR4302547.5771583/94‑1 (MQ=60)
                                                                      CGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGA                      <  SRR4302547.5526642/94‑1 (MQ=60)
                                                                      CGCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGA                      <  SRR4302547.3637272/94‑1 (MQ=60)
                                                                       GCAGTCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAG                     <  SRR4302547.5890761/94‑1 (MQ=60)
                                                                       GCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAG                     <  SRR4302547.5975521/94‑1 (MQ=60)
                                                                       GCAGGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAG                     <  SRR4302547.1855521/94‑1 (MQ=60)
                                                                        CAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGA                    >  SRR4302547.1511132/1‑94 (MQ=60)
                                                                          gtctcCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAA                  <  SRR4302547.42996/89‑1 (MQ=60)
                                                                          GGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAA                  >  SRR4302547.1582622/1‑94 (MQ=60)
                                                                          GGCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAA                  >  SRR4302547.2873128/1‑94 (MQ=60)
                                                                           GCTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAAT                 <  SRR4302547.4476243/94‑1 (MQ=60)
                                                                            CTTCGCGCGTGTCCTGCATGGAGATGCTGGCATGCGCGCCGCTGTTAATGGATGACCTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATC                <  SRR4302547.1923422/94‑1 (MQ=60)
                                                                            CTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACTGGCGATTTGAAGGCATTAATTGATGAGAAATC                <  SRR4302547.5518690/94‑1 (MQ=60)
                                                                            CTTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATC                >  SRR4302547.2324834/1‑94 (MQ=60)
                                                                            CTTCGCCCCTGTTCTGCAGGGAGATGCTGGCAGGCGCACCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATC                <  SRR4302547.2483288/94‑1 (MQ=60)
                                                                             TTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCG               <  SRR4302547.5770239/94‑1 (MQ=60)
                                                                             TTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCG               <  SRR4302547.3785537/94‑1 (MQ=60)
                                                                             TTCGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCG               <  SRR4302547.4091519/94‑1 (MQ=60)
                                                                               CGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGC             >  SRR4302547.3146418/1‑94 (MQ=60)
                                                                               CGCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGC             >  SRR4302547.5720441/1‑94 (MQ=60)
                                                                                GCGCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGGG            >  SRR4302547.311912/1‑94 (MQ=60)
                                                                                  GCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCT          >  SRR4302547.2829169/1‑94 (MQ=60)
                                                                                  GCCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCT          <  SRR4302547.295575/94‑1 (MQ=60)
                                                                                   CCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTG         >  SRR4302547.3174715/1‑94 (MQ=60)
                                                                                   CCTGTTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTG         >  SRR4302547.2238363/1‑94 (MQ=60)
                                                                                       TTCTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTG     <  SRR4302547.4668308/94‑1 (MQ=60)
                                                                                         CTGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGCC   >  SRR4302547.6703182/1‑94 (MQ=60)
                                                                                          TGCATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGCCG  <  SRR4302547.6390412/94‑1 (MQ=60)
                                                                                            |                                                                                           
GAAGATTTCGCCCCGCTGGCGGCGATCAAAGAGTACATCCGTCTGAAGCTGGAAGTCTCACGCGATTATCCGCAGGCTTCGCGCCTGTTCTGTATGGAGATGCTGGCAGGCGCGCCGCTGTTAATGGATGAACTGACGGGCGATTTGAAGGCATTAATTGATGAGAAATCGGCGCTGATTGCCG  >  NC_000913/1073720‑1073903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑