Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,236,913 T→C S40S (AGT→AGC)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,236,9130TC83.0% 436.0 / 82.9 189S40S (AGT→AGC) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base T (18/14);  new base C (85/71);  total (104/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 202 total aligned reads displayed.

GTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCA  >  NC_000913/1236821‑1237002
                                                                                            |                                                                                         
gtggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGt                                                                                           <  2:14431/93‑1 (MQ=255)
  ggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAgcgc                                                                                         <  2:4211898/93‑1 (MQ=255)
    tAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCcg                                                                                       <  1:1071063/93‑1 (MQ=255)
    tAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCcg                                                                                       <  2:1173235/93‑1 (MQ=255)
    tAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTAATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCcg                                                                                       <  1:653222/93‑1 (MQ=255)
       gCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGa                                                                                    <  2:2632777/93‑1 (MQ=255)
        cGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGaa                                                                                   <  1:2940475/93‑1 (MQ=255)
        cGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGaa                                                                                   <  1:48404/93‑1 (MQ=255)
        cGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGaa                                                                                   <  2:5622328/93‑1 (MQ=255)
         gTTGCCAGCGCCTGGTACTTAAATCAGGCAGGGCATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAt                                                                                  >  1:2320350/1‑93 (MQ=255)
         gTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAAt                                                                                  >  2:2021791/1‑93 (MQ=255)
         gTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAt                                                                                  >  2:5558690/1‑93 (MQ=255)
         gTTGCCAGCGCCTGGTACTTAAATCAGGCAGAACATGAGGTCGCCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAt                                                                                  <  2:5874999/93‑1 (MQ=255)
          ttGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATg                                                                                 <  1:5672726/93‑1 (MQ=255)
          ttGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATg                                                                                 <  2:320514/93‑1 (MQ=255)
              cAGCGCGTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCgg                                                                             <  1:6552300/93‑1 (MQ=255)
              cAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCgg                                                                             <  1:16722/93‑1 (MQ=255)
              cAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCgg                                                                             >  2:3746979/1‑93 (MQ=255)
               aGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCggg                                                                            >  1:87474/1‑93 (MQ=255)
                  gcCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCaa                                                                         >  1:5552677/1‑93 (MQ=255)
                   ccTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCaaa                                                                        >  1:6258503/1‑93 (MQ=255)
                    cTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAAt                                                                       >  2:1133348/1‑93 (MQ=255)
                     tGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAAtc                                                                      >  2:3797792/1‑93 (MQ=255)
                      ggTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAAtct                                                                     >  1:1135964/1‑93 (MQ=255)
                      ggTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAAtct                                                                     <  1:2429011/93‑1 (MQ=255)
                      ggTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAAtct                                                                     <  1:2490543/93‑1 (MQ=255)
                        tACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTcc                                                                   >  1:4653961/1‑93 (MQ=255)
                         aCTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGGAACCAGCGCCGCGCATGCCGGGGCAATCTccc                                                                  >  1:2119978/1‑93 (MQ=255)
                         aCTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTccc                                                                  >  1:2582865/1‑93 (MQ=255)
                         aCTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTccc                                                                  >  1:5156004/1‑93 (MQ=255)
                          cTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTcccc                                                                 <  2:5124870/93‑1 (MQ=255)
                           ttAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTccccc                                                                >  1:1812348/1‑93 (MQ=255)
                            tAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCg                                                               >  1:4170802/1‑93 (MQ=255)
                            tAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCg                                                               >  2:2481614/1‑93 (MQ=255)
                                 caggcaggACATGAGGTCACCGTCATTGATCGGGAGCAGGGGGCCGCCCTGGAAATCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGatat                                                          >  2:6039371/1‑93 (MQ=255)
                                    gcaggACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATAtgct                                                       <  2:4821541/93‑1 (MQ=255)
                                      aggACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATAtgctgc                                                     <  2:5829417/93‑1 (MQ=255)
                                        gACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGTATGCCGGGCAAATCTCCCCCGGATATGCTGCAc                                                   >  1:2696946/1‑93 (MQ=255)
                                          cATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCg                                                 >  1:5851271/1‑93 (MQ=255)
                                            tGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTg                                               >  2:4013004/1‑93 (MQ=255)
                                             gAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTgg                                              >  2:598994/1‑93 (MQ=255)
                                             gAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTgg                                              <  2:3134362/93‑1 (MQ=255)
                                             gAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTgg                                              <  2:4644741/93‑1 (MQ=255)
                                              aGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTggg                                             >  2:1585421/1‑93 (MQ=255)
                                              aGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTggg                                             >  1:442417/1‑93 (MQ=255)
                                               ggTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGggc                                            <  1:1272970/93‑1 (MQ=255)
                                                 tCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGggcgg                                          >  2:3689825/1‑93 (MQ=255)
                                                    ccGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCAc                                       >  1:6524503/1‑93 (MQ=255)
                                                     cGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCAcc                                      <  2:430697/93‑1 (MQ=255)
                                                      gTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCa                                     >  1:1737425/1‑93 (MQ=255)
                                                       tCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAg                                    <  1:4212496/93‑1 (MQ=255)
                                                       tCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGTCAAATCGCCCCCGGATATGCTGCACCGTGGGCGGCACGAg                                    <  2:2039384/93‑1 (MQ=255)
                                                        cATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGGGCCGCGAATGCAGGGCAAATCTCCCCCGGGTACGCTGCACCGTGGGCGGCACCAgg                                   >  1:4490012/1‑93 (MQ=255)
                                                         aTTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgt                                  <  1:2006022/93‑1 (MQ=255)
                                                         aTTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgt                                  >  1:5166491/1‑93 (MQ=255)
                                                         aTTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgt                                  <  1:3524178/93‑1 (MQ=255)
                                                          ttGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgtg                                 <  1:4370610/93‑1 (MQ=255)
                                                          ttGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgtg                                 <  2:6529243/93‑1 (MQ=255)
                                                          ttGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgtg                                 <  2:1957665/93‑1 (MQ=255)
                                                           tGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgtgt                                >  1:135255/1‑93 (MQ=255)
                                                             aTCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGc                              >  1:527848/1‑93 (MQ=255)
                                                              tCGGGAGCCGTGGTCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGcc                             <  1:1163527/93‑1 (MQ=255)
                                                              tCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGcc                             <  2:4083936/93‑1 (MQ=255)
                                                                gggAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCtt                           <  1:3764345/93‑1 (MQ=255)
                                                                 ggAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCttt                          >  2:6306951/1‑93 (MQ=255)
                                                                    gCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTaaa                       >  2:4447987/1‑93 (MQ=255)
                                                                     ccGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTaaaa                      <  2:1265246/93‑1 (MQ=255)
                                                                        ggggCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCg                   <  1:2464235/93‑1 (MQ=255)
                                                                         gggCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGa                  >  2:4956364/1‑93 (MQ=255)
                                                                          ggCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAt                 <  1:3993698/93‑1 (MQ=255)
                                                                          ggCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAt                 >  1:561793/1‑93 (MQ=255)
                                                                          ggCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGAACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAt                 >  2:5189033/1‑93 (MQ=255)
                                                                           gccgccCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAtt                <  1:4008196/93‑1 (MQ=255)
                                                                           gccgccCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAtt                >  2:5437043/1‑93 (MQ=255)
                                                                            ccgccCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTa               <  1:3099983/93‑1 (MQ=255)
                                                                            ccgccCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTa               <  2:827269/93‑1 (MQ=255)
                                                                             cgccCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTaa              >  1:5515081/1‑93 (MQ=255)
                                                                              gccCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTaaa             >  2:5454874/1‑93 (MQ=255)
                                                                              gccCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTaaa             <  2:6341362/93‑1 (MQ=255)
                                                                              gccCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTaaa             <  1:3168172/93‑1 (MQ=255)
                                                                              gccCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTaaa             <  2:685591/93‑1 (MQ=255)
                                                                               cccTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAAt            <  1:6439262/93‑1 (MQ=255)
                                                                               cccTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAAt            <  1:5378164/93‑1 (MQ=255)
                                                                                ccTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATg           >  1:2792042/1‑93 (MQ=255)
                                                                                ccTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATg           <  2:2998862/93‑1 (MQ=255)
                                                                                 cTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATgg          >  2:6599526/1‑93 (MQ=255)
                                                                                  tGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGa         >  1:2717251/1‑93 (MQ=255)
                                                                                  tGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGa         <  2:3528351/93‑1 (MQ=255)
                                                                                  tGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGGGCCTTTAAAAGCGATTAAATGGa         <  1:5337696/93‑1 (MQ=255)
                                                                                   ggAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGAt        >  1:2650414/1‑93 (MQ=255)
                                                                                   ggAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGAt        >  1:5963189/1‑93 (MQ=255)
                                                                                   ggAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGAt        >  2:2577524/1‑93 (MQ=255)
                                                                                    gAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATg       >  1:6016558/1‑93 (MQ=255)
                                                                                    gAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATg       <  2:4246799/93‑1 (MQ=255)
                                                                                     aaaCCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGt      <  2:2193542/93‑1 (MQ=255)
                                                                                      aaCCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGtt     >  1:3352050/1‑93 (MQ=255)
                                                                                        ccAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTcc   <  1:148504/93‑1 (MQ=255)
                                                                                        ccAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTcc   <  1:678467/93‑1 (MQ=255)
                                                                                         cAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCGCCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCa  <  1:41118/93‑1 (MQ=255)
                                                                                         cAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGACTTTAAAAGCGATTAAATGGATGTTCCa  >  2:3657687/1‑93 (MQ=255)
                                                                                            |                                                                                         
GTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCA  >  NC_000913/1236821‑1237002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGC  >  NC_000913/1236820‑1237004
                                                                                             |                                                                                           
TGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGC                                                                                             <  SRR4302547.4092845/94‑1 (MQ=60)
TGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGC                                                                                             <  SRR4302547.4367131/94‑1 (MQ=60)
TGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGC                                                                                             <  SRR4302547.5332527/94‑1 (MQ=60)
TGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGC                                                                                             <  SRR4302547.5264939/94‑1 (MQ=60)
 GTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTG                                                                                            >  SRR4302547.174779/1‑94 (MQ=60)
 GTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCG                                                                                            >  SRR4302547.2209589/1‑94 (MQ=60)
 GTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCG                                                                                            >  SRR4302547.5107945/1‑94 (MQ=60)
  TGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGC                                                                                           <  SRR4302547.1995030/94‑1 (MQ=60)
    GTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCG                                                                                         <  SRR4302547.5712851/94‑1 (MQ=60)
     TAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGC                                                                                        <  SRR4302547.1092402/94‑1 (MQ=60)
     TAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGC                                                                                        <  SRR4302547.211810/94‑1 (MQ=60)
     TAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGC                                                                                        >  SRR4302547.4166935/1‑94 (MQ=60)
     TAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGC                                                                                        <  SRR4302547.1369724/94‑1 (MQ=60)
     TAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTAATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGC                                                                                        <  SRR4302547.665256/94‑1 (MQ=60)
      AGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCG                                                                                       <  SRR4302547.253008/94‑1 (MQ=60)
         CGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGGCATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAT                                                                                    >  SRR4302547.2367373/1‑94 (MQ=60)
         CGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAT                                                                                    <  SRR4302547.49305/94‑1 (MQ=60)
         CGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAT                                                                                    <  SRR4302547.2999063/94‑1 (MQ=60)
         CGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAAT                                                                                    <  SRR4302547.1158079/94‑1 (MQ=60)
          GTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATG                                                                                   >  SRR4302547.1512684/1‑94 (MQ=60)
          GTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATG                                                                                   >  SRR4302547.5484895/1‑94 (MQ=60)
           TTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGC                                                                                  <  SRR4302547.5789857/94‑1 (MQ=60)
             GCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCG                                                                                <  SRR4302547.5375420/94‑1 (MQ=60)
               CAGCGCGTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGG                                                                              <  SRR4302547.6688674/94‑1 (MQ=60)
               CAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGG                                                                              >  SRR4302547.89098/1‑94 (MQ=60)
               CAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGG                                                                              <  SRR4302547.17039/94‑1 (MQ=60)
                AGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGC                                                                             <  SRR4302547.4618849/94‑1 (MQ=60)
                AGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGA                                                                             >  SRR4302547.525449/1‑94 (MQ=60)
                  CGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAA                                                                           >  SRR4302547.3493143/1‑94 (MQ=60)
                  CGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAA                                                                           >  SRR4302547.5667230/1‑94 (MQ=60)
                  CGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAA                                                                           >  SRR4302547.4079002/1‑94 (MQ=60)
                   GCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAA                                                                          >  SRR4302547.78183/1‑94 (MQ=60)
                   GCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAA                                                                          >  SRR4302547.6388515/1‑94 (MQ=60)
                      TGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCT                                                                       >  SRR4302547.2337389/1‑94 (MQ=60)
                      TGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCT                                                                       >  SRR4302547.1158901/1‑94 (MQ=60)
                      TGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCT                                                                       >  SRR4302547.2689423/1‑94 (MQ=60)
                      TGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGACGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCT                                                                       >  SRR4302547.65530/1‑94 (MQ=60)
                       GGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTC                                                                      <  SRR4302547.4568953/94‑1 (MQ=60)
                       GGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTC                                                                      <  SRR4302547.2540855/94‑1 (MQ=60)
                       GGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTC                                                                      >  SRR4302547.201641/1‑94 (MQ=60)
                       GGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTC                                                                      <  SRR4302547.2478159/94‑1 (MQ=60)
                        GTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCC                                                                     >  SRR4302547.4499499/1‑94 (MQ=60)
                        GTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCC                                                                     >  SRR4302547.437058/1‑94 (MQ=60)
                        GTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCC                                                                     >  SRR4302547.4750354/1‑94 (MQ=60)
                        GTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCC                                                                     >  SRR4302547.6057871/1‑94 (MQ=60)
                         TACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGGAACCAGCGCCGCGCATGCCGGGGCAATCTCCC                                                                    >  SRR4302547.2162231/1‑94 (MQ=60)
                         TACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCC                                                                    >  SRR4302547.2634991/1‑94 (MQ=60)
                         TACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCC                                                                    >  SRR4302547.5262174/1‑94 (MQ=60)
                           CTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCC                                                                  >  SRR4302547.5612375/1‑94 (MQ=60)
                           CTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCC                                                                  >  SRR4302547.1848155/1‑94 (MQ=60)
                           CTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCC                                                                  >  SRR4302547.620813/1‑94 (MQ=60)
                           CTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCC                                                                  <  SRR4302547.1161762/94‑1 (MQ=60)
                            TTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCG                                                                 >  SRR4302547.4255763/1‑94 (MQ=60)
                             TAAATCAGTCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCTCCCTGGAAACCAGCGCCGCGAATGCCGCGCAAATCTCCCCCGG                                                                <  SRR4302547.3759056/94‑1 (MQ=60)
                             TAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGG                                                                >  SRR4302547.5648738/1‑94 (MQ=60)
                              AAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAAACAGCGGCGCGAATGCAGGGCAAATCTCTCCCGGA                                                               >  SRR4302547.3179273/1‑94 (MQ=60)
                                      CAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGC                                                       <  SRR4302547.1847327/94‑1 (MQ=60)
                                       AGGACATGGGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCA                                                      >  SRR4302547.6676168/1‑94 (MQ=60)
                                       AGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCA                                                      >  SRR4302547.5388081/1‑94 (MQ=60)
                                        GGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGTATGCCGGGCAAATCTCCCCCGGATATGCTGCAC                                                     >  SRR4302547.2751088/1‑94 (MQ=60)
                                          ACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCG                                                   >  SRR4302547.5972310/1‑94 (MQ=60)
                                            ATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTG                                                 <  SRR4302547.5936555/94‑1 (MQ=60)
                                              GAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGG                                               >  SRR4302547.450658/1‑94 (MQ=60)
                                                GGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCG                                             <  SRR4302547.2979235/94‑1 (MQ=60)
                                                GGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCG                                             <  SRR4302547.1298606/94‑1 (MQ=60)
                                                 GTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGG                                            <  SRR4302547.2557086/94‑1 (MQ=60)
                                                    ACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCAC                                         >  SRR4302547.6660330/1‑94 (MQ=60)
                                                      CGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCA                                       >  SRR4302547.1771574/1‑94 (MQ=60)
                                                        TCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGG                                     <  SRR4302547.4298471/94‑1 (MQ=60)
                                                        TCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGGGCCGCGAATGCAGGGCAAATCTCCCCCGGGTACGCTGCACCGTGGGCGGCACCAGG                                     >  SRR4302547.4582765/1‑94 (MQ=60)
                                                         CATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGT                                    >  SRR4302547.5272863/1‑94 (MQ=60)
                                                          ATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTG                                   >  SRR4302547.1006409/1‑94 (MQ=60)
                                                          ATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTG                                   <  SRR4302547.158743/94‑1 (MQ=60)
                                                          ATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTG                                   <  SRR4302547.2045935/94‑1 (MQ=60)
                                                          ATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTG                                   >  SRR4302547.2770151/1‑94 (MQ=60)
                                                          ATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTG                                   <  SRR4302547.3596114/94‑1 (MQ=60)
                                                           TTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGT                                  <  SRR4302547.4460400/94‑1 (MQ=60)
                                                           TTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGT                                  <  SRR4302547.6608162/94‑1 (MQ=60)
                                                           TTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGT                                  >  SRR4302547.137698/1‑94 (MQ=60)
                                                            TGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTG                                 <  SRR4302547.3914247/94‑1 (MQ=60)
                                                             GATCGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGC                                >  SRR4302547.537387/1‑94 (MQ=60)
                                                              ATCGGGAGCCGGGGGACGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGACATGCTGCACCGTGGGCGGCACCAGGTGcgac                               >  SRR4302547.3865044/1‑90 (MQ=60)
                                                               TCGGGAGCCGTGGTCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCT                              <  SRR4302547.1187067/94‑1 (MQ=60)
                                                                CGGGAGCCGGGTGCCGCACTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGGCTT                             >  SRR4302547.2182198/1‑94 (MQ=60)
                                                                CGGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTT                             >  SRR4302547.2395800/1‑94 (MQ=60)
                                                                CGGGAGCCGGGGGCCGCCCGGGAAACCAGCGCCGCGAATGCCGGGCAAATCGCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTT                             <  SRR4302547.1297616/94‑1 (MQ=60)
                                                                 GGGAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTT                            <  SRR4302547.3841124/94‑1 (MQ=60)
                                                                     GCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAA                        >  SRR4302547.5064369/1‑94 (MQ=60)
                                                                     GCCGGGGGCCGCCCTGGAAACCAGGGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGGAGCACCGGGGGTGCCTTTAGAA                        >  SRR4302547.2493182/1‑94 (MQ=60)
                                                                        GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCG                     >  SRR4302547.3813743/1‑94 (MQ=60)
                                                                        GGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGGGGCGCCAGGTGTGCCTTTAAAAGCG                     >  SRR4302547.5908088/1‑94 (MQ=60)
                                                                         GGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGA                    <  SRR4302547.2514045/94‑1 (MQ=60)
                                                                         GGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGA                    >  SRR4302547.6661403/1‑94 (MQ=60)
                                                                          GGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAT                   >  SRR4302547.571913/1‑94 (MQ=60)
                                                                          GGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGAT                   <  SRR4302547.271078/94‑1 (MQ=60)
                                                                           GGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATT                  <  SRR4302547.4074896/94‑1 (MQ=60)
                                                                           GGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGCGCCTTTAAAAGCGATT                  >  SRR4302547.4196170/1‑94 (MQ=60)
                                                                            GCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGTTGTGCCTTTAAAAGCGATTA                 <  SRR4302547.6620462/94‑1 (MQ=60)
                                                                            GCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTA                 <  SRR4302547.4089663/94‑1 (MQ=60)
                                                                            GCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTA                 <  SRR4302547.3083061/94‑1 (MQ=60)
                                                                             CCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAA                >  SRR4302547.5628932/1‑94 (MQ=60)
                                                                             CCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAA                <  SRR4302547.3161384/94‑1 (MQ=60)
                                                                              CGCCCTGGAAACCCGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTCCACCGGGGGCGGCACCAGGTGAGCATTTAAAAGCGATTAGA               >  SRR4302547.6095508/1‑94 (MQ=60)
                                                                              CGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAA               >  SRR4302547.3354508/1‑94 (MQ=60)
                                                                               GCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAAT              <  SRR4302547.3231213/94‑1 (MQ=60)
                                                                                CCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATG             <  SRR4302547.5489184/94‑1 (MQ=60)
                                                                                CCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATG             >  SRR4302547.2847952/1‑94 (MQ=60)
                                                                                CCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATG             <  SRR4302547.6573451/94‑1 (MQ=60)
                                                                                 CCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGG            <  SRR4302547.6777441/94‑1 (MQ=60)
                                                                                  CTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGA           >  SRR4302547.4295850/1‑94 (MQ=60)
                                                                                  CTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGA           >  SRR4302547.2771800/1‑94 (MQ=60)
                                                                                  CTGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGA           >  SRR4302547.4884347/1‑94 (MQ=60)
                                                                                   TGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGAT          >  SRR4302547.1269291/1‑94 (MQ=60)
                                                                                   TGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGAT          >  SRR4302547.6086861/1‑94 (MQ=60)
                                                                                   TGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGAT          >  SRR4302547.2703709/1‑94 (MQ=60)
                                                                                   TGGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGGGCCTTTAAAAGCGATTAAATGGAT          <  SRR4302547.5447728/94‑1 (MQ=60)
                                                                                    GGAAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATG         >  SRR4302547.6141430/1‑94 (MQ=60)
                                                                                      AAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTT       >  SRR4302547.2483024/1‑94 (MQ=60)
                                                                                      AAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTT       >  SRR4302547.3420030/1‑94 (MQ=60)
                                                                                      AAACCAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTT       >  SRR4302547.5762571/1‑94 (MQ=60)
                                                                                         CCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCA    <  SRR4302547.151208/94‑1 (MQ=60)
                                                                                         CCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCA    <  SRR4302547.690966/94‑1 (MQ=60)
                                                                                          CAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCGCCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAG   <  SRR4302547.41873/94‑1 (MQ=60)
                                                                                          CAGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAG   <  SRR4302547.2816085/94‑1 (MQ=60)
                                                                                           AGCGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGC  <  SRR4302547.4879639/94‑1 (MQ=60)
                                                                                             |                                                                                           
TGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGC  >  NC_000913/1236820‑1237004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑