Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,237,202 A→G T137A (ACG→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,237,2020AG99.5% 710.6 / ‑4.5 202T137A (ACG→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (97/104);  total (97/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 215 total aligned reads displayed.

GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTG  >  NC_000913/1237113‑1237291
                                                                                         |                                                                                         
ggATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGgcgc                                                                                        <  2:2214031/93‑1 (MQ=255)
ggATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGc                                                                                        <  2:6099121/93‑1 (MQ=255)
   tGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGc                                                                                     <  2:2990531/93‑1 (MQ=255)
     gTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCaa                                                                                   <  1:4986533/93‑1 (MQ=255)
      tGCGTCTGGCGTAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAc                                                                                  >  1:5724887/1‑93 (MQ=255)
      tGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAc                                                                                  <  2:536898/93‑1 (MQ=255)
           cTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTc                                                                             <  2:5154020/93‑1 (MQ=255)
            tGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATGTTCAGTATGAAGGGCGTCAGGGTGGGGCTCTGCAACTGTTcc                                                                            <  2:3409996/93‑1 (MQ=255)
             ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCg                                                                           <  2:1357916/93‑1 (MQ=255)
             ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCg                                                                           >  1:1635720/1‑93 (MQ=255)
              gCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGt                                                                          <  1:2153299/93‑1 (MQ=255)
               cGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTa                                                                         <  2:5089674/93‑1 (MQ=255)
                 gAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTAcc                                                                       >  1:2602854/1‑93 (MQ=255)
                 gAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGAGTCAGGGTGGGGCGCTGCAACTGTTCCGTAcc                                                                       >  1:595288/1‑93 (MQ=255)
                  aaTACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCg                                                                      <  2:1901146/93‑1 (MQ=255)
                   aTACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGa                                                                     <  1:5627697/93‑1 (MQ=255)
                    tACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaa                                                                    >  1:604347/1‑93 (MQ=255)
                     aCAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaac                                                                   >  2:1626045/1‑93 (MQ=255)
                     aCAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaac                                                                   <  2:2402462/93‑1 (MQ=255)
                     aCAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaac                                                                   >  1:2559700/1‑93 (MQ=255)
                        gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGTCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacaac                                                                >  2:4593223/1‑93 (MQ=255)
                        gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacagc                                                                >  1:3037120/1‑91 (MQ=255)
                        gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacaac                                                                >  2:1466063/1‑93 (MQ=255)
                        gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacaac                                                                >  2:1980451/1‑93 (MQ=255)
                        gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacaac                                                                >  1:4198227/1‑93 (MQ=255)
                         ccGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAacaaca                                                               <  1:2248784/93‑1 (MQ=255)
                          cGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAg                                                              >  2:3708394/1‑93 (MQ=255)
                           gTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGt                                                             >  2:5854579/1‑93 (MQ=255)
                            tGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTa                                                            <  2:2170329/93‑1 (MQ=255)
                            tGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTa                                                            <  2:4559165/93‑1 (MQ=255)
                             gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                           >  1:2825426/1‑93 (MQ=255)
                             gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                           <  1:4726112/93‑1 (MQ=255)
                             gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                           <  2:4703508/93‑1 (MQ=255)
                             gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                           >  2:39207/1‑93 (MQ=255)
                              aTTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGAATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATg                                                          >  1:3257860/1‑93 (MQ=255)
                               ttGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGa                                                         <  2:1661836/93‑1 (MQ=255)
                                tGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaa                                                        <  1:1909572/93‑1 (MQ=255)
                                 gCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaaa                                                       <  2:1395828/93‑1 (MQ=255)
                                 gCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaaa                                                       >  2:5214032/1‑93 (MQ=255)
                                  ccTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaaaa                                                      <  2:1747249/93‑1 (MQ=255)
                                  ccTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaaaa                                                      <  2:3575684/93‑1 (MQ=255)
                                   cTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt                                                     >  1:4501625/1‑93 (MQ=255)
                                   cTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt                                                     >  1:1554942/1‑93 (MQ=255)
                                   cTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt                                                     >  2:2628605/1‑93 (MQ=255)
                                   cTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt                                                     <  1:636628/93‑1 (MQ=255)
                                    tGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATg                                                    >  2:4323179/1‑93 (MQ=255)
                                    tGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATg                                                    >  1:1719666/1‑93 (MQ=255)
                                    tGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATg                                                    >  1:5578381/1‑93 (MQ=255)
                                     gAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGc                                                   >  1:802094/1‑93 (MQ=255)
                                     gAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGc                                                   <  1:1518070/93‑1 (MQ=255)
                                      aaaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCg                                                  >  1:523638/1‑93 (MQ=255)
                                        acaaTTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                <  1:5450741/90‑1 (MQ=255)
                                        aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGTCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGGACAACAGTATGAAGATGCGAc                                                >  1:1182259/1‑93 (MQ=255)
                                        aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                <  1:2381154/93‑1 (MQ=255)
                                        aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                <  2:3190415/93‑1 (MQ=255)
                                        aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                <  2:2433599/93‑1 (MQ=255)
                                         gCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAcc                                               >  2:5449355/1‑93 (MQ=255)
                                          cATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAccc                                              >  1:2465199/1‑93 (MQ=255)
                                            ttGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCcgc                                            <  2:5280374/93‑1 (MQ=255)
                                              gcgcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGTGa                                          <  2:785380/93‑1 (MQ=255)
                                              gcgcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGa                                          <  1:4051691/93‑1 (MQ=255)
                                              gcgcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGa                                          <  2:5935982/93‑1 (MQ=255)
                                                 cgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGatat                                       >  2:3124373/1‑93 (MQ=255)
                                                  gcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                      >  1:4823254/1‑93 (MQ=255)
                                                  gcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                      <  1:1192415/93‑1 (MQ=255)
                                                    cGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGc                                    <  2:4251594/93‑1 (MQ=255)
                                                    cGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGc                                    <  1:5035038/93‑1 (MQ=255)
                                                    cGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGc                                    >  2:5421386/1‑93 (MQ=255)
                                                     gAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGcc                                   >  1:102665/1‑93 (MQ=255)
                                                       aaCCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAACATGCGACCCGCGATATCGCCgc                                 >  1:6115018/1‑92 (MQ=255)
                                                       aaCCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGt                                 <  1:3954596/93‑1 (MQ=255)
                                                             tatTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGa                           >  1:2910641/1‑93 (MQ=255)
                                                             tatTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGa                           >  1:2030830/1‑93 (MQ=255)
                                                             aatTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGa                           <  1:224677/92‑1 (MQ=255)
                                                              atTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGaa                          <  2:4347000/93‑1 (MQ=255)
                                                              atTCAGTATGAAGAGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGaa                          >  2:4178300/1‑93 (MQ=255)
                                                                tCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGa                        <  1:4084158/93‑1 (MQ=255)
                                                                   gTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGc                     >  2:4469154/1‑93 (MQ=255)
                                                                   gTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGc                     <  2:4847951/93‑1 (MQ=255)
                                                                    tATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGcc                    <  2:5534683/93‑1 (MQ=255)
                                                                     aTGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCg                   <  1:1949271/93‑1 (MQ=255)
                                                                       gAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGc                 >  2:295574/1‑93 (MQ=255)
                                                                       gAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGc                 >  1:1534880/1‑93 (MQ=255)
                                                                       gAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGc                 <  2:1112303/93‑1 (MQ=255)
                                                                       gAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGc                 >  1:4520148/1‑93 (MQ=255)
                                                                        aaGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCTGCg                >  2:4785984/1‑93 (MQ=255)
                                                                         aGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGt               <  2:90501/93‑1 (MQ=255)
                                                                          gggCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTa              >  1:1694824/1‑93 (MQ=255)
                                                                           ggCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTAc             <  1:2894324/93‑1 (MQ=255)
                                                                             cGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCg           <  1:5254801/93‑1 (MQ=255)
                                                                              gTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGt          <  2:1001632/93‑1 (MQ=255)
                                                                               tCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTa         >  2:1063024/1‑93 (MQ=255)
                                                                               tCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTa         >  1:3883440/1‑93 (MQ=255)
                                                                               tCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTa         >  2:738949/1‑93 (MQ=255)
                                                                                 aGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATc       >  2:4482213/1‑93 (MQ=255)
                                                                                  gggTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCa      >  2:3802447/1‑93 (MQ=255)
                                                                                  gggTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCa      <  1:4284971/93‑1 (MQ=255)
                                                                                  gggTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCa      <  1:2767386/93‑1 (MQ=255)
                                                                                     tGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgct   <  2:1221263/93‑1 (MQ=255)
                                                                                     tGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgct   >  1:3624910/1‑93 (MQ=255)
                                                                                         |                                                                                         
GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTG  >  NC_000913/1237113‑1237291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTG  >  NC_000913/1237110‑1237294
                                                                                            |                                                                                            
GGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGC                                                                                             <  SRR4302532.4158344/94‑1 (MQ=60)
   GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                          >  SRR4302532.94506/1‑94 (MQ=60)
   GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                          <  SRR4302532.6080379/94‑1 (MQ=60)
   GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                          <  SRR4302532.2866836/94‑1 (MQ=60)
    GATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTG                                                                                         >  SRR4302532.4227449/1‑94 (MQ=60)
    GATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTG                                                                                         >  SRR4302532.2009715/1‑94 (MQ=60)
        GTGCGTCTGGCGTAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                     >  SRR4302532.5924277/1‑94 (MQ=60)
        GTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                     >  SRR4302532.5250626/1‑94 (MQ=60)
        GTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                     <  SRR4302532.5157471/94‑1 (MQ=60)
        GTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                     <  SRR4302532.2981099/94‑1 (MQ=60)
           CGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGT                                                                                  >  SRR4302532.5790607/1‑94 (MQ=60)
            GTCTGGCGGGATCCACCCGTGATTGCCTGAAAACATTGCGCGCCGAAACCAATATTCAGTATGAAAGGCGTCAGGGTGGGGGGCTGCAACTGTT                                                                                 >  SRR4302532.4230701/1‑94 (MQ=60)
             TCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTC                                                                                <  SRR4302532.1210911/94‑1 (MQ=60)
              CTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCC                                                                               <  SRR4302532.3167633/94‑1 (MQ=60)
               TGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCG                                                                              >  SRR4302532.1691089/1‑94 (MQ=60)
                GGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGT                                                                             >  SRR4302532.4595892/1‑94 (MQ=60)
                GGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGT                                                                             <  SRR4302532.1792418/94‑1 (MQ=60)
                 GCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTA                                                                            <  SRR4302532.2225277/94‑1 (MQ=60)
                   GGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACC                                                                          >  SRR4302532.2689898/1‑94 (MQ=60)
                   GGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGAGTCAGGGTGGGGCGCTGCAACTGTTCCGTACC                                                                          >  SRR4302532.616076/1‑94 (MQ=60)
                    GAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCG                                                                         >  SRR4302532.1772290/1‑94 (MQ=60)
                      ATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAA                                                                       >  SRR4302532.6353877/1‑94 (MQ=60)
                      ATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAA                                                                       <  SRR4302532.5822993/94‑1 (MQ=60)
                      ATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAA                                                                       >  SRR4302532.625462/1‑94 (MQ=60)
                       TACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAAC                                                                      >  SRR4302532.2645091/1‑94 (MQ=60)
                          AGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAGC                                                                   >  SRR4302532.3137337/1‑94 (MQ=60)
                          AGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAAC                                                                   >  SRR4302532.4339123/1‑94 (MQ=60)
                            CCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAG                                                                 <  SRR4302532.5431906/94‑1 (MQ=60)
                            CCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAG                                                                 <  SRR4302532.2322873/94‑1 (MQ=60)
                            CCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAG                                                                 <  SRR4302532.2323439/94‑1 (MQ=60)
                               TGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAT                                                              >  SRR4302532.2919942/1‑94 (MQ=60)
                                TATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGAATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATG                                                             >  SRR4302532.3366111/1‑94 (MQ=60)
                                GATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATG                                                             <  SRR4302532.4887392/94‑1 (MQ=60)
                                  TTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAA                                                           <  SRR4302532.6359830/94‑1 (MQ=60)
                                   TGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAA                                                          <  SRR4302532.1972977/94‑1 (MQ=60)
                                     CCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAT                                                        >  SRR4302532.4655315/1‑94 (MQ=60)
                                     CCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAT                                                        >  SRR4302532.1607518/1‑94 (MQ=60)
                                     CCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAT                                                        <  SRR4302532.1577869/94‑1 (MQ=60)
                                      CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                       <  SRR4302532.2687266/94‑1 (MQ=60)
                                      CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                       >  SRR4302532.4978124/1‑94 (MQ=60)
                                      CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                       <  SRR4302532.658820/94‑1 (MQ=60)
                                      CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                       >  SRR4302532.1777607/1‑94 (MQ=60)
                                      CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                       >  SRR4302532.5772060/1‑94 (MQ=60)
                                      CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                       >  SRR4302532.5886435/1‑94 (MQ=60)
                                       TGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGC                                                      >  SRR4302532.829498/1‑94 (MQ=60)
                                       TGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGC                                                      >  SRR4302532.789428/1‑94 (MQ=60)
                                        GAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCG                                                     <  SRR4302532.1569343/94‑1 (MQ=60)
                                        GAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCG                                                     >  SRR4302532.541913/1‑94 (MQ=60)
                                        GAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCG                                                     <  SRR4302532.2071857/94‑1 (MQ=60)
                                          AAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGTCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGGACAACAGTATGAAGATGCGAC                                                   >  SRR4302532.1221751/1‑94 (MQ=60)
                                           acaATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACC                                                  <  SRR4302532.5639655/91‑1 (MQ=60)
                                           AGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACC                                                  <  SRR4302532.477385/94‑1 (MQ=60)
                                           AGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACC                                                  >  SRR4302532.2705598/1‑94 (MQ=60)
                                           AGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACC                                                  <  SRR4302532.2460493/94‑1 (MQ=60)
                                            GCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCC                                                 >  SRR4302532.2547253/1‑94 (MQ=60)
                                                 GCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGAT                                            <  SRR4302532.4188015/94‑1 (MQ=60)
                                                  CGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATA                                           <  SRR4302532.5498683/94‑1 (MQ=60)
                                                   GCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATACGACCCGCGATAT                                          >  SRR4302532.264038/1‑94 (MQ=60)
                                                    CGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATC                                         >  SRR4302532.4989296/1‑94 (MQ=60)
                                                     GCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAATTGCGACCCGCGATATCG                                        >  SRR4302532.5665906/1‑94 (MQ=60)
                                                     GCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCG                                        <  SRR4302532.1232284/94‑1 (MQ=60)
                                                       CGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCC                                      <  SRR4302532.2441068/94‑1 (MQ=60)
                                                       CGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCC                                      <  SRR4302532.5207724/94‑1 (MQ=60)
                                                       CGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCC                                      >  SRR4302532.106224/1‑94 (MQ=60)
                                                       CGAAACCAATATTCAGTATGAAGGGCGTCAGGGCGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATATGACCCGCGATATCGCC                                      >  SRR4302532.1913084/1‑94 (MQ=60)
                                                         AAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAACATGCGACCCGCGATATCGCCGC                                    >  SRR4302532.6326060/1‑94 (MQ=60)
                                                          AACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTG                                   <  SRR4302532.4087857/94‑1 (MQ=60)
                                                          AACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTG                                   <  SRR4302532.1589707/94‑1 (MQ=60)
                                                          AACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTG                                   <  SRR4302532.5613954/94‑1 (MQ=60)
                                                          AACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTG                                   <  SRR4302532.1342702/94‑1 (MQ=60)
                                                           ACCAATATTCAGTATGAAGGGCGTCAGGGCGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAACATGCGACCCGCGATATCGCCGTGC                                  >  SRR4302532.6177924/1‑94 (MQ=60)
                                                             CAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTG                                <  SRR4302532.5191550/94‑1 (MQ=60)
                                                              AATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGG                               >  SRR4302532.2108897/1‑94 (MQ=60)
                                                               ATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGA                              >  SRR4302532.2098518/1‑94 (MQ=60)
                                                               ATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGA                              >  SRR4302532.3007697/1‑94 (MQ=60)
                                                                TATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAA                             <  SRR4302532.232355/94‑1 (MQ=60)
                                                                AATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAA                             <  SRR4302532.232353/94‑1 (MQ=60)
                                                                   TCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGAT                          <  SRR4302532.4221275/94‑1 (MQ=60)
                                                                       TATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCG                      >  SRR4302532.4051841/1‑94 (MQ=60)
                                                                       TATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCG                      <  SRR4302532.3780845/94‑1 (MQ=60)
                                                                       TATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTGCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCG                      <  SRR4302532.4674249/94‑1 (MQ=60)
                                                                       TATGAAGGGCGTCAGGGCGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAACCGCGATATCGCCGTGCTGGAAGATGACG                      >  SRR4302532.514985/1‑94 (MQ=60)
                                                                        ATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGG                     >  SRR4302532.304550/1‑94 (MQ=60)
                                                                        ATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGG                     <  SRR4302532.2014068/94‑1 (MQ=60)
                                                                        ATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGG                     >  SRR4302532.6307710/1‑94 (MQ=60)
                                                                         TGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGC                    >  SRR4302532.1586742/1‑94 (MQ=60)
                                                                         TGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGC                    >  SRR4302532.4674449/1‑94 (MQ=60)
                                                                            AGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTC                 >  SRR4302532.2302476/1‑94 (MQ=60)
                                                                            AGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTA                 >  SRR4302532.2993645/1‑94 (MQ=60)
                                                                            AGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTA                 >  SRR4302532.1752041/1‑94 (MQ=60)
                                                                              GGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACC               <  SRR4302532.2990971/94‑1 (MQ=60)
                                                                                CGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGCCCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGT             <  SRR4302532.4018661/94‑1 (MQ=60)
                                                                                CGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGT             <  SRR4302532.5435978/94‑1 (MQ=60)
                                                                                CGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGT             >  SRR4302532.2906968/1‑94 (MQ=60)
                                                                                CGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATAGCCGTGCTGGAAGATGCCGGCGTACCGT             >  SRR4302532.1704174/1‑94 (MQ=60)
                                                                                CGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGGATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGGACCGG             >  SRR4302532.1704173/1‑94 (MQ=60)
                                                                                 GTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTA            >  SRR4302532.4014504/1‑94 (MQ=60)
                                                                                 GTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTA            >  SRR4302532.2476052/1‑94 (MQ=60)
                                                                                    AGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCA         <  SRR4302532.1230538/94‑1 (MQ=60)
                                                                                     GGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAG        <  SRR4302532.470466/94‑1 (MQ=60)
                                                                                     GGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAG        <  SRR4302532.4429432/94‑1 (MQ=60)
                                                                                     GGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAG        <  SRR4302532.1673363/94‑1 (MQ=60)
                                                                                     GGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAG        <  SRR4302532.2860304/94‑1 (MQ=60)
                                                                                      GGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGC       >  SRR4302532.4196007/1‑94 (MQ=60)
                                                                                      GGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGC       <  SRR4302532.4094893/94‑1 (MQ=60)
                                                                                       GTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCT      >  SRR4302532.3746393/1‑94 (MQ=60)
                                                                                       GTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCT      <  SRR4302532.3705677/94‑1 (MQ=60)
                                                                                         GGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGC    >  SRR4302532.4326287/1‑94 (MQ=60)
                                                                                           GGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCCACCCGGGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTG  <  SRR4302532.3248320/94‑1 (MQ=60)
                                                                                            |                                                                                            
GGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTG  >  NC_000913/1237110‑1237294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑