Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,237,202 A→G T137A (ACG→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,237,2020AG100.0% 755.9 / NA 211T137A (ACG→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (119/92);  total (119/92)

Only 100 of 222 total aligned reads displayed.

CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTG  >  NC_000913/1237112‑1237291
                                                                                          |                                                                                         
 ggATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGgcgc                                                                                        <  1:48479/93‑1 (MQ=255)
 ggATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGgcgc                                                                                        <  1:3281975/93‑1 (MQ=255)
  gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCt                                                                                       >  1:1140340/1‑93 (MQ=255)
  gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCt                                                                                       >  1:6615897/1‑93 (MQ=255)
  gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCt                                                                                       >  2:3117915/1‑93 (MQ=255)
  gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCt                                                                                       <  1:2296144/93‑1 (MQ=255)
    tGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGc                                                                                     >  1:3502743/1‑93 (MQ=255)
      gTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCaa                                                                                   <  1:3657687/93‑1 (MQ=255)
       tGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGGGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAc                                                                                  >  2:5560036/1‑93 (MQ=255)
       tGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAc                                                                                  <  1:6061998/93‑1 (MQ=255)
       tGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAc                                                                                  >  1:4642119/1‑93 (MQ=255)
        gCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACt                                                                                 >  2:2506339/1‑93 (MQ=255)
         cttCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGTGTGGGGCGCTGCAACTg                                                                                <  2:5144774/91‑1 (MQ=255)
         cGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTg                                                                                >  2:804353/1‑93 (MQ=255)
         cGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTg                                                                                <  1:1849488/93‑1 (MQ=255)
          gTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGt                                                                               <  1:907543/93‑1 (MQ=255)
            cTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTc                                                                             <  1:5688784/93‑1 (MQ=255)
              ggCGGAATCCAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCg                                                                           <  1:5189033/93‑1 (MQ=255)
               gCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGt                                                                          >  1:3048272/1‑93 (MQ=255)
                cGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTa                                                                         >  2:3981473/1‑93 (MQ=255)
                cGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTa                                                                         <  2:1461049/93‑1 (MQ=255)
                  gAAGACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTAcc                                                                       <  2:3179385/93‑1 (MQ=255)
                   aaTACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCg                                                                      >  2:5926399/1‑93 (MQ=255)
                     tACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaa                                                                    <  1:26023/93‑1 (MQ=255)
                       cAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaaca                                                                  >  2:3728712/1‑93 (MQ=255)
                        aGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCGACTGTTCCGTACCGaacaa                                                                 >  1:3831430/1‑93 (MQ=255)
                         gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacaac                                                                >  1:4485934/1‑93 (MQ=255)
                         gCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGaacaac                                                                >  2:672774/1‑93 (MQ=255)
                          ccGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAacaaca                                                               >  2:1749745/1‑93 (MQ=255)
                          ccGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAacaaca                                                               <  1:6331621/93‑1 (MQ=255)
                             tGATTGCCTGAAAGCATTGCGCGCCTAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTa                                                            <  2:6078297/93‑1 (MQ=255)
                             tGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCTCTGCAACTGTTCCGTACCGAACAACAGTa                                                            <  2:4653961/93‑1 (MQ=255)
                             tGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTa                                                            <  1:4574458/93‑1 (MQ=255)
                             tGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTa                                                            <  1:5551602/93‑1 (MQ=255)
                              gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                           >  2:3933949/1‑93 (MQ=255)
                              gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                           >  1:1896672/1‑93 (MQ=255)
                               aTTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATg                                                          >  1:4040180/1‑93 (MQ=255)
                               aTTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATg                                                          <  2:5204042/93‑1 (MQ=255)
                                ttGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGa                                                         >  1:6108541/1‑93 (MQ=255)
                                  gCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaaa                                                       >  1:1201505/1‑93 (MQ=255)
                                   ccTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGaaaa                                                      >  1:402210/1‑93 (MQ=255)
                                     tGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATg                                                    >  2:3312909/1‑93 (MQ=255)
                                       aaaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCg                                                  >  1:1707545/1‑93 (MQ=255)
                                        aaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGa                                                 <  2:6540069/93‑1 (MQ=255)
                                        aaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGa                                                 >  2:6282930/1‑93 (MQ=255)
                                        aaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGa                                                 >  2:1152680/1‑93 (MQ=255)
                                         aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                >  1:2155868/1‑93 (MQ=255)
                                         aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                >  1:794159/1‑93 (MQ=255)
                                         aGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAc                                                <  2:2942724/93‑1 (MQ=255)
                                          gCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAcc                                               >  1:4102248/1‑93 (MQ=255)
                                          gCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAcc                                               >  1:1534725/1‑93 (MQ=255)
                                           cATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAccc                                              <  1:5016453/93‑1 (MQ=255)
                                           cATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAccc                                              <  2:614997/93‑1 (MQ=255)
                                              tGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCcgcg                                           <  2:1365918/93‑1 (MQ=255)
                                                cgcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGat                                         >  1:3306204/1‑93 (MQ=255)
                                                 gcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGata                                        <  1:1728627/93‑1 (MQ=255)
                                                 gcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGata                                        <  2:4296754/93‑1 (MQ=255)
                                                   gcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCTCGATATc                                      >  2:5878268/1‑93 (MQ=255)
                                                   gcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                      <  2:1812348/93‑1 (MQ=255)
                                                   gcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                      <  1:3211624/93‑1 (MQ=255)
                                                   gcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                      >  2:498895/1‑93 (MQ=255)
                                                     cGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGc                                    >  1:2010512/1‑93 (MQ=255)
                                                     cGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGc                                    >  2:3524774/1‑93 (MQ=255)
                                                      gAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGcc                                   >  2:1100720/1‑93 (MQ=255)
                                                      gAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGcc                                   >  2:969938/1‑93 (MQ=255)
                                                       aaaCCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCg                                  <  2:1204349/93‑1 (MQ=255)
                                                       aaaCCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCg                                  >  1:6569034/1‑93 (MQ=255)
                                                       aaaCCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCg                                  <  1:4949798/93‑1 (MQ=255)
                                                         aCCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTg                                >  1:5136017/1‑93 (MQ=255)
                                                          ccAATATTCAGTATGAAGGGCGTCAGGGTGGTGCGCTGCAACTGTTCCTTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGc                               <  2:1476197/93‑1 (MQ=255)
                                                          ccAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGc                               <  2:1275312/93‑1 (MQ=255)
                                                          ccAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGc                               >  1:5940271/1‑93 (MQ=255)
                                                           cAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                              <  2:2166162/93‑1 (MQ=255)
                                                           cAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                              <  2:5371144/93‑1 (MQ=255)
                                                           cAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                              >  1:835397/1‑93 (MQ=255)
                                                            aaTATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTg                             <  2:2209276/93‑1 (MQ=255)
                                                                ttCAGTATGAAGGGCGTCAGGGTTGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAg                         >  2:1687914/1‑93 (MQ=255)
                                                                 tCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGa                        <  1:2322059/93‑1 (MQ=255)
                                                                  cAGTATGAAGGACGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGAt                       <  1:4393618/93‑1 (MQ=255)
                                                                     tATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGcc                    <  2:2348206/93‑1 (MQ=255)
                                                                     tATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGcc                    >  2:2558767/1‑93 (MQ=255)
                                                                      aTGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCg                   >  1:3396859/1‑93 (MQ=255)
                                                                       tGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCgg                  <  1:5851060/93‑1 (MQ=255)
                                                                       tGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCgg                  >  2:4952390/1‑93 (MQ=255)
                                                                           gggCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTa              <  2:1594294/93‑1 (MQ=255)
                                                                           gggCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTa              >  1:6131353/1‑93 (MQ=255)
                                                                           gggCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTa              <  2:4490012/93‑1 (MQ=255)
                                                                            ggCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTAc             <  2:4823670/93‑1 (MQ=255)
                                                                               gTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGt          >  1:1276362/1‑93 (MQ=255)
                                                                                tCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTa         >  2:2410956/1‑93 (MQ=255)
                                                                                tCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTa         >  2:6337090/1‑93 (MQ=255)
                                                                                  aGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATc       >  2:3334513/1‑93 (MQ=255)
                                                                                  aGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATc       >  1:1432410/1‑93 (MQ=255)
                                                                                    ggTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAg     >  1:4282167/1‑93 (MQ=255)
                                                                                    ggTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAg     >  2:880575/1‑93 (MQ=255)
                                                                                     gTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgc    <  2:5960459/93‑1 (MQ=255)
                                                                                     gTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgc    <  1:666437/93‑1 (MQ=255)
                                                                                      tGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgct   <  1:2055046/93‑1 (MQ=255)
                                                                                       ggggCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgctg  >  2:5801908/1‑93 (MQ=255)
                                                                                       ggggCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgctg  >  1:3797265/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTG  >  NC_000913/1237112‑1237291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGG  >  NC_000913/1237109‑1237295
                                                                                             |                                                                                             
GGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGG                                                                                               <  SRR4302547.4268588/94‑1 (MQ=60)
 GGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGTGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGC                                                                                              >  SRR4302547.6783194/1‑94 (MQ=60)
   CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGC                                                                                            <  SRR4302547.3890923/94‑1 (MQ=60)
   CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGC                                                                                            >  SRR4302547.5950585/1‑94 (MQ=60)
    GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                           >  SRR4302547.1163368/1‑94 (MQ=60)
    GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                           >  SRR4302547.4606917/1‑94 (MQ=60)
    GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                           <  SRR4302547.3347777/94‑1 (MQ=60)
    GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                           <  SRR4302547.49382/94‑1 (MQ=60)
    GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                           >  SRR4302547.6736449/1‑94 (MQ=60)
    GGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCT                                                                                           >  SRR4302547.6753607/1‑94 (MQ=60)
     GATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTG                                                                                          <  SRR4302547.2342669/94‑1 (MQ=60)
      ATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCTCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGC                                                                                         <  SRR4302547.1692737/94‑1 (MQ=60)
      ATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGC                                                                                         >  SRR4302547.3574224/1‑94 (MQ=60)
         GTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                      <  SRR4302547.3732312/94‑1 (MQ=60)
         GTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                      >  SRR4302547.4738263/1‑94 (MQ=60)
         GTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAAC                                                                                      >  SRR4302547.4737354/1‑94 (MQ=60)
          TGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACT                                                                                     >  SRR4302547.3344143/1‑94 (MQ=60)
          TGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACT                                                                                     <  SRR4302547.6157803/94‑1 (MQ=60)
          TGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACT                                                                                     <  SRR4302547.6187738/94‑1 (MQ=60)
           GCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTG                                                                                    >  SRR4302547.6265432/1‑94 (MQ=60)
           GCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTG                                                                                    >  SRR4302547.5212995/1‑94 (MQ=60)
            CGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGT                                                                                   <  SRR4302547.1886044/94‑1 (MQ=60)
             GTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTT                                                                                  <  SRR4302547.925035/94‑1 (MQ=60)
             GTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTT                                                                                  >  SRR4302547.1190228/1‑94 (MQ=60)
               CTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCC                                                                                >  SRR4302547.905026/1‑94 (MQ=60)
               CTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCC                                                                                <  SRR4302547.5806258/94‑1 (MQ=60)
                 GGCGGAATCCAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGT                                                                              <  SRR4302547.5295823/94‑1 (MQ=60)
                 GGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGT                                                                              >  SRR4302547.3108917/1‑94 (MQ=60)
                    GGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACC                                                                           >  SRR4302547.338298/1‑94 (MQ=60)
                     GAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCG                                                                          <  SRR4302547.3483135/94‑1 (MQ=60)
                     GAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCG                                                                          >  SRR4302547.1565799/1‑94 (MQ=60)
                       ATACAGCCGTGTTTGCCTGCAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAA                                                                        >  SRR4302547.3435919/1‑94 (MQ=60)
                        TACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAAC                                                                       <  SRR4302547.26501/94‑1 (MQ=60)
                        TACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAAC                                                                       >  SRR4302547.2576377/1‑94 (MQ=60)
                          CAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCGACTGTTCCGTACCGAACAA                                                                     >  SRR4302547.3909477/1‑94 (MQ=60)
                           AGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAAC                                                                    >  SRR4302547.4578600/1‑94 (MQ=60)
                             CCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAG                                                                  <  SRR4302547.6463431/94‑1 (MQ=60)
                               GTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTA                                                                >  SRR4302547.2714173/1‑94 (MQ=60)
                                TGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAT                                                               <  SRR4302547.4669118/94‑1 (MQ=60)
                                TGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAT                                                               >  SRR4302547.1934181/1‑94 (MQ=60)
                                TGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTAT                                                               <  SRR4302547.5666140/94‑1 (MQ=60)
                                 GATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATG                                                              <  SRR4302547.87468/94‑1 (MQ=60)
                                 GATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATG                                                              <  SRR4302547.1751214/94‑1 (MQ=60)
                                 GATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATG                                                              >  SRR4302547.4122225/1‑94 (MQ=60)
                                  ATTGCCTGAAAGCATTGCGCGCCGAATCTAAAATACAGGATGAAGGGCGTCAGGGTGGGTCTCTGCAACTGTTCCGTACCGAACAACAGTATGA                                                             <  SRR4302547.5879647/94‑1 (MQ=60)
                                  ATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGA                                                             >  SRR4302547.6235248/1‑94 (MQ=60)
                                    TGCCTGAAAGCATTGCGCGGCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAcgattcaa                                                           >  SRR4302547.1606234/1‑86 (MQ=60)
                                    TGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAA                                                           >  SRR4302547.2068081/1‑94 (MQ=60)
                                    TGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAA                                                           >  SRR4302547.1225757/1‑94 (MQ=60)
                                     GCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAA                                                          >  SRR4302547.409678/1‑94 (MQ=60)
                                       CTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATG                                                        >  SRR4302547.6702206/1‑94 (MQ=60)
                                        TGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGC                                                       >  SRR4302547.1959253/1‑94 (MQ=60)
                                        TGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGC                                                       >  SRR4302547.847613/1‑94 (MQ=60)
                                         GAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCG                                                      >  SRR4302547.1741136/1‑94 (MQ=60)
                                          AAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGA                                                     >  SRR4302547.4485100/1‑94 (MQ=60)
                                          AAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGA                                                     >  SRR4302547.932890/1‑94 (MQ=60)
                                           AAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAC                                                    >  SRR4302547.809185/1‑94 (MQ=60)
                                           AAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGAC                                                    >  SRR4302547.2198929/1‑94 (MQ=60)
                                            AGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACC                                                   >  SRR4302547.1565268/1‑94 (MQ=60)
                                            AGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACC                                                   >  SRR4302547.4185659/1‑94 (MQ=60)
                                              CATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCG                                                 <  SRR4302547.5774305/94‑1 (MQ=60)
                                              CATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCG                                                 <  SRR4302547.5120141/94‑1 (MQ=60)
                                               ATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGC                                                <  SRR4302547.4094572/94‑1 (MQ=60)
                                                TTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCG                                               <  SRR4302547.4406752/94‑1 (MQ=60)
                                                  GCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGAT                                             >  SRR4302547.3372847/1‑94 (MQ=60)
                                                   CCCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGCTGGCGCGCTGCAACGGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATA                                            <  SRR4302547.2838786/94‑1 (MQ=60)
                                                    GCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATAT                                           <  SRR4302547.1762588/94‑1 (MQ=60)
                                                    GCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATAT                                           <  SRR4302547.4876226/94‑1 (MQ=60)
                                                    GCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGCCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCCCGATAT                                           <  SRR4302547.3798583/94‑1 (MQ=60)
                                                     CGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATC                                          >  SRR4302547.5634485/1‑94 (MQ=60)
                                                      GCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCG                                         >  SRR4302547.3226555/1‑94 (MQ=60)
                                                      GCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCG                                         <  SRR4302547.6787899/94‑1 (MQ=60)
                                                      GCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCG                                         <  SRR4302547.3275691/94‑1 (MQ=60)
                                                      GCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCG                                         <  SRR4302547.2430729/94‑1 (MQ=60)
                                                       CCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGC                                        >  SRR4302547.2050519/1‑94 (MQ=60)
                                                       CCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGC                                        >  SRR4302547.3621509/1‑94 (MQ=60)
                                                        CGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCC                                       <  SRR4302547.4256971/94‑1 (MQ=60)
                                                         GAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCG                                      >  SRR4302547.6705806/1‑94 (MQ=60)
                                                         GAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCG                                      >  SRR4302547.5084702/1‑94 (MQ=60)
                                                          AAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGT                                     <  SRR4302547.5052287/94‑1 (MQ=60)
                                                          AAACCAATATTCAGTATGAAGGGCGTCAGGGAGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCCACGCGCGATATCGCCGT                                     >  SRR4302547.893732/1‑94 (MQ=60)
                                                           AACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTG                                    >  SRR4302547.781774/1‑94 (MQ=60)
                                                           AACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTG                                    >  SRR4302547.5241832/1‑94 (MQ=60)
                                                            ACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGC                                   >  SRR4302547.6063441/1‑94 (MQ=60)
                                                            ACCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGC                                   >  SRR4302547.2295957/1‑94 (MQ=60)
                                                             CCAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCT                                  >  SRR4302547.851340/1‑94 (MQ=60)
                                                              CAATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTG                                 >  SRR4302547.6729482/1‑94 (MQ=60)
                                                               AATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGG                                >  SRR4302547.1890049/1‑94 (MQ=60)
                                                               AATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGG                                >  SRR4302547.2348978/1‑94 (MQ=60)
                                                                ATATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGA                               <  SRR4302547.4861809/94‑1 (MQ=60)
                                                                 TATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAA                              >  SRR4302547.5260733/1‑94 (MQ=60)
                                                                  ATTCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAG                             >  SRR4302547.1857933/1‑94 (MQ=60)
                                                                    TCAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGAT                           <  SRR4302547.2369127/94‑1 (MQ=60)
                                                                     CAGTATGAAGGGCGTCAGGGTTGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATG                          <  SRR4302547.1374149/94‑1 (MQ=60)
                                                                     CAGTATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATG                          <  SRR4302547.4997409/94‑1 (MQ=60)
                                                                     CAGTATGAAGGACGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATG                          <  SRR4302547.4484041/94‑1 (MQ=60)
                                                                        TATGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCG                       >  SRR4302547.3465977/1‑94 (MQ=60)
                                                                        TATGAAGGAGGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCG                       <  SRR4302547.3878380/94‑1 (MQ=60)
                                                                          TGAAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGC                     <  SRR4302547.5972095/94‑1 (MQ=60)
                                                                            AAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGT                   <  SRR4302547.6195030/94‑1 (MQ=60)
                                                                            AAGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGT                   >  SRR4302547.6250794/1‑94 (MQ=60)
                                                                             AGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTA                  >  SRR4302547.2084439/1‑94 (MQ=60)
                                                                             AGGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTA                  >  SRR4302547.6258535/1‑94 (MQ=60)
                                                                              GGGCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCAGTGCTGGAAGATGCCGGCGTAC                 <  SRR4302547.3369833/94‑1 (MQ=60)
                                                                                GCGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCG               >  SRR4302547.4109352/1‑94 (MQ=60)
                                                                                 CGTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGT              >  SRR4302547.1302064/1‑94 (MQ=60)
                                                                                  GTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTA             <  SRR4302547.611267/94‑1 (MQ=60)
                                                                                  GTCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTA             >  SRR4302547.4182071/1‑94 (MQ=60)
                                                                                   TCAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTAT            >  SRR4302547.1285552/1‑94 (MQ=60)
                                                                                    CAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATC           >  SRR4302547.6027159/1‑94 (MQ=60)
                                                                                    CAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATC           <  SRR4302547.1491593/94‑1 (MQ=60)
                                                                                    CAGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATC           >  SRR4302547.1461047/1‑94 (MQ=60)
                                                                                     AGGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCA          >  SRR4302547.3832063/1‑94 (MQ=60)
                                                                                      GGGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAG         >  SRR4302547.4370132/1‑94 (MQ=60)
                                                                                       GGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGC        <  SRR4302547.4397965/94‑1 (MQ=60)
                                                                                       GGTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGCACCATATCCGC        >  SRR4302547.2125486/1‑94 (MQ=60)
                                                                                        GTGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCT       <  SRR4302547.678717/94‑1 (MQ=60)
                                                                                         TGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTG      >  SRR4302547.3874679/1‑94 (MQ=60)
                                                                                         TGGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTG      <  SRR4302547.2096038/94‑1 (MQ=60)
                                                                                          GGGGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGC     >  SRR4302547.6250481/1‑94 (MQ=60)
                                                                                          GGGGCGCTGCAACTGTGCCGGACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGC     <  SRR4302547.5567586/94‑1 (MQ=60)
                                                                                            GGCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTG   <  SRR4302547.2070237/94‑1 (MQ=60)
                                                                                             GCGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTTCTGGAAGATGCCGGCGTACTGTATCAGCTGCTGG  <  SRR4302547.3850742/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGG  >  NC_000913/1237109‑1237295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑