Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,277,250 C→T A24V (GCG→GTG)  narK → nitrite extrusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,277,2500CT80.2% 504.3 / 102.5 207A24V (GCG→GTG) narKnitrite extrusion protein
Reads supporting (aligned to +/- strand):  ref base C (16/25);  new base T (84/82);  total (100/107)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 213 total aligned reads displayed.

AAACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGT  >  NC_000913/1277159‑1277342
                                                                                           |                                                                                            
 aaCGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTgtgt                                                                                            >  2:2323908/1‑93 (MQ=255)
 aaCGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGt                                                                                            <  2:3999293/93‑1 (MQ=255)
  aCGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGtt                                                                                           >  2:5016648/1‑93 (MQ=255)
   cGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTc                                                                                          >  1:1409513/1‑93 (MQ=255)
   cGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTc                                                                                          <  1:2712256/93‑1 (MQ=255)
    gAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCt                                                                                         >  1:4150011/1‑93 (MQ=255)
    gAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCt                                                                                         >  1:3065588/1‑93 (MQ=255)
    gAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCt                                                                                         <  1:4908778/93‑1 (MQ=255)
      gTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTgg                                                                                       >  2:5883963/1‑93 (MQ=255)
        aTCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGca                                                                                     >  2:4656789/1‑93 (MQ=255)
        aTCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGca                                                                                     >  2:1443508/1‑93 (MQ=255)
        aTCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGca                                                                                     >  2:2683875/1‑93 (MQ=255)
         tCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGcaa                                                                                    >  2:4333784/1‑93 (MQ=255)
          cAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGcaac                                                                                   <  2:4441156/93‑1 (MQ=255)
           agagGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGcaaca                                                                                  <  1:3147167/93‑1 (MQ=255)
              ggTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAAcg                                                                               <  2:2169411/93‑1 (MQ=255)
              ggTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAAcg                                                                               <  1:4823044/93‑1 (MQ=255)
              ggTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAAcg                                                                               >  2:4707989/1‑93 (MQ=255)
                tgtCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAAcgcg                                                                             >  2:3633164/1‑93 (MQ=255)
                tgtCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAAcgcg                                                                             >  2:5558617/1‑93 (MQ=255)
                  tCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGt                                                                           >  1:849038/1‑93 (MQ=255)
                    tATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCa                                                                         <  1:4470505/93‑1 (MQ=255)
                    tATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCa                                                                         >  1:1161580/1‑93 (MQ=255)
                    tATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCa                                                                         <  2:6243678/93‑1 (MQ=255)
                     aTGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCaa                                                                        <  1:6246622/93‑1 (MQ=255)
                      tGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAAc                                                                       >  2:5047298/1‑93 (MQ=255)
                       gAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACg                                                                      >  2:5984075/1‑93 (MQ=255)
                         gTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTa                                                                    >  2:3358969/1‑93 (MQ=255)
                          tCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTAt                                                                   <  2:830642/93‑1 (MQ=255)
                          tCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTAt                                                                   <  2:5217925/93‑1 (MQ=255)
                           cACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTAtt                                                                  <  2:2088293/93‑1 (MQ=255)
                           cACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTAtt                                                                  <  2:2928760/93‑1 (MQ=255)
                              tCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGcc                                                               <  1:15623/93‑1 (MQ=255)
                               cATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCa                                                              <  2:3123806/93‑1 (MQ=255)
                                aTCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAg                                                             >  1:3456450/1‑93 (MQ=255)
                                 tCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGc                                                            <  1:1931041/93‑1 (MQ=255)
                                    gCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGc                                                         <  1:785428/93‑1 (MQ=255)
                                      ccccGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCaa                                                       <  2:1601217/93‑1 (MQ=255)
                                       cccGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAAc                                                      >  1:5388472/1‑93 (MQ=255)
                                        ccGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAAcc                                                     >  1:2290580/1‑93 (MQ=255)
                                        ccGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAAcc                                                     >  1:4324181/1‑93 (MQ=255)
                                        ccGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAAcc                                                     <  2:808145/93‑1 (MQ=255)
                                         cGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCt                                                    >  1:5252264/1‑93 (MQ=255)
                                         cGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCt                                                    <  1:2125500/93‑1 (MQ=255)
                                           aaaGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCtgt                                                  <  2:5326561/93‑1 (MQ=255)
                                            aaGGGCTACTGGAGCTGTCCTTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCtgtg                                                 >  1:6464106/1‑93 (MQ=255)
                                            aaGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCtgtg                                                 <  2:4803326/93‑1 (MQ=255)
                                            aaGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCtgtg                                                 <  2:3717317/93‑1 (MQ=255)
                                            aaGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCtgtg                                                 <  2:2449606/93‑1 (MQ=255)
                                             aGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTgg                                                >  1:1983050/1‑93 (MQ=255)
                                              gggCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGa                                               <  1:4971814/93‑1 (MQ=255)
                                              gggCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGa                                               <  2:5133619/93‑1 (MQ=255)
                                              gggCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGa                                               >  2:5304298/1‑93 (MQ=255)
                                                gCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGAtt                                             >  2:869686/1‑93 (MQ=255)
                                                gCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGAtt                                             <  1:4661349/93‑1 (MQ=255)
                                                 cTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGAttt                                            >  2:1941541/1‑93 (MQ=255)
                                                  tACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTc                                           >  2:3463647/1‑93 (MQ=255)
                                                  tACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTc                                           <  2:5380226/93‑1 (MQ=255)
                                                  tACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTc                                           >  1:222274/1‑93 (MQ=255)
                                                   aCTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTcc                                          <  2:5667686/93‑1 (MQ=255)
                                                     tGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGt                                        >  2:1362226/1‑93 (MQ=255)
                                                      ggAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGtt                                       <  2:2025655/93‑1 (MQ=255)
                                                      ggAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGta                                       >  2:6380932/1‑92 (MQ=255)
                                                       gAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTc                                      <  1:3870620/93‑1 (MQ=255)
                                                        aGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTcc                                     >  2:473012/1‑93 (MQ=255)
                                                         gCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTccc                                    >  2:2585161/1‑93 (MQ=255)
                                                         gCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTccc                                    <  2:75067/93‑1 (MQ=255)
                                                         gCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTccc                                    <  1:2577700/93‑1 (MQ=255)
                                                            gTCATTACAGATTGGCGACCGGCAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGACAGCCGCAACCTGTGGATTTCCGTTCCCttt                                 >  2:946643/1‑91 (MQ=255)
                                                            gTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGt                                 >  1:3556043/1‑93 (MQ=255)
                                                             tCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTc                                <  2:383678/93‑1 (MQ=255)
                                                             tCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTc                                <  2:2127704/93‑1 (MQ=255)
                                                             tCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTc                                >  2:1020298/1‑93 (MQ=255)
                                                              cATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTct                               >  1:2544237/1‑93 (MQ=255)
                                                                  aCAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTctgctg                           <  2:6555904/93‑1 (MQ=255)
                                                                    aGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTctgctgct                         >  2:6287790/1‑93 (MQ=255)
                                                                    aGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTctgctgct                         <  1:6146304/93‑1 (MQ=255)
                                                                     gATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTctgctgctg                        <  1:2733858/93‑1 (MQ=255)
                                                                      aTTGGCGACCGGGAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTgg                       >  1:4377477/1‑93 (MQ=255)
                                                                         ggCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGt                    <  2:1732562/93‑1 (MQ=255)
                                                                          gCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGtt                   <  1:474454/93‑1 (MQ=255)
                                                                            gACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGtttt                 <  1:5952832/93‑1 (MQ=255)
                                                                            gACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGtttt                 <  1:5665934/93‑1 (MQ=255)
                                                                             aCCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTg                >  1:1340373/1‑93 (MQ=255)
                                                                               cGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCg              >  2:6407590/1‑93 (MQ=255)
                                                                                ggAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGt             >  2:4876185/1‑93 (MQ=255)
                                                                                 gAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTa            <  1:2971502/93‑1 (MQ=255)
                                                                                 gAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCGTGTCTGCTGCTGGCGTTTTGCGTa            <  2:3870862/93‑1 (MQ=255)
                                                                                  aaGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTAt           >  2:784070/1‑93 (MQ=255)
                                                                                   aGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATg          >  1:5786918/1‑93 (MQ=255)
                                                                                   aGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATg          >  1:3323860/1‑93 (MQ=255)
                                                                                     aTCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGa        <  1:102488/93‑1 (MQ=255)
                                                                                     aTCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGa        <  2:6436016/93‑1 (MQ=255)
                                                                                     aTCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGa        <  2:1398149/93‑1 (MQ=255)
                                                                                     aTCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGa        >  1:5579585/1‑93 (MQ=255)
                                                                                      tCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGAt       <  1:4255179/93‑1 (MQ=255)
                                                                                      tCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGAt       <  1:5216566/93‑1 (MQ=255)
                                                                                        cTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGAtgt     >  2:3852883/1‑93 (MQ=255)
                                                                                        cTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGACGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGAtgt     >  1:2400181/1‑93 (MQ=255)
                                                                                           cGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGAtgttgt  <  1:2266872/93‑1 (MQ=255)
                                                                                           |                                                                                            
AAACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGT  >  NC_000913/1277159‑1277342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTT  >  NC_000913/1277158‑1277343
                                                                                            |                                                                                             
GAAACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTG                                                                                              >  SRR4302547.1072408/1‑94 (MQ=60)
GAAACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTG                                                                                              >  SRR4302547.2553281/1‑94 (MQ=60)
  AACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTT                                                                                            >  SRR4302547.5090468/1‑94 (MQ=60)
   ACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTC                                                                                           >  SRR4302547.4589369/1‑94 (MQ=60)
   ACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTC                                                                                           >  SRR4302547.1437706/1‑94 (MQ=60)
    CGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCT                                                                                          <  SRR4302547.2766707/94‑1 (MQ=60)
    CGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCT                                                                                          >  SRR4302547.3126532/1‑94 (MQ=60)
    CGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCT                                                                                          >  SRR4302547.4234530/1‑94 (MQ=60)
     GAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTG                                                                                         <  SRR4302547.5010434/94‑1 (MQ=60)
       GTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGC                                                                                       <  SRR4302547.2457906/94‑1 (MQ=60)
        TATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCA                                                                                      >  SRR4302547.631263/1‑94 (MQ=60)
         ATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAA                                                                                     <  SRR4302547.3195436/94‑1 (MQ=60)
          TCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAAC                                                                                    <  SRR4302547.5514449/94‑1 (MQ=60)
          TCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAAC                                                                                    >  SRR4302547.38567/1‑94 (MQ=60)
          TCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAAC                                                                                    <  SRR4302547.820694/94‑1 (MQ=60)
            AGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAA                                                                                  <  SRR4302547.3209699/94‑1 (MQ=60)
             GAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAAC                                                                                 >  SRR4302547.3501591/1‑94 (MQ=60)
              AGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACG                                                                                >  SRR4302547.217445/1‑94 (MQ=60)
              AGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACG                                                                                <  SRR4302547.213311/94‑1 (MQ=60)
               GGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGC                                                                               <  SRR4302547.4923004/94‑1 (MQ=60)
                GTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCG                                                                              <  SRR4302547.6455548/94‑1 (MQ=60)
                  GTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGT                                                                            >  SRR4302547.865250/1‑94 (MQ=60)
                    CTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCA                                                                          >  SRR4302547.1185077/1‑94 (MQ=60)
                     TATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAA                                                                         <  SRR4302547.4562871/94‑1 (MQ=60)
                     TATGAGTCACTCAGCCGCCCCCGAAAGGTCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAA                                                                         <  SRR4302547.5719537/94‑1 (MQ=60)
                      ATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAAC                                                                        <  SRR4302547.6376377/94‑1 (MQ=60)
                         AGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTA                                                                     <  SRR4302547.5760840/94‑1 (MQ=60)
                          GTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTAT                                                                    <  SRR4302547.4451827/94‑1 (MQ=60)
                              CTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCC                                                                >  SRR4302547.3059674/1‑94 (MQ=60)
                               TCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCA                                                               <  SRR4302547.6055968/94‑1 (MQ=60)
                               TCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCA                                                               <  SRR4302547.15914/94‑1 (MQ=60)
                                CATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAG                                                              >  SRR4302547.3526916/1‑94 (MQ=60)
                                  TCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCC                                                            <  SRR4302547.1969323/94‑1 (MQ=60)
                                   CCGCCCCCGAAAGGGCTACTGGAGCTGACATTACAGATTCGCGACCGGAACATCCTTTGTTCTAGCAACAACCCGGTCgaactattcgcagaca                                                           >  SRR4302547.922811/1‑78 (MQ=47)
                                    CGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGC                                                          <  SRR4302547.2407805/94‑1 (MQ=60)
                                    CGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCCACGTATTGCCAGCCGC                                                          >  SRR4302547.446951/1‑94 (MQ=60)
                                     GCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCA                                                         <  SRR4302547.800285/94‑1 (MQ=60)
                                      CCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAA                                                        >  SRR4302547.2659286/1‑94 (MQ=60)
                                      CCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAA                                                        <  SRR4302547.2292668/94‑1 (MQ=60)
                                       CCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAAC                                                       >  SRR4302547.5499741/1‑94 (MQ=60)
                                       CCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAAC                                                       >  SRR4302547.5351658/1‑94 (MQ=60)
                                        CCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACC                                                      >  SRR4302547.2336987/1‑94 (MQ=60)
                                        CCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACC                                                      >  SRR4302547.4413197/1‑94 (MQ=60)
                                         CCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCT                                                     >  SRR4302547.1585091/1‑94 (MQ=60)
                                         CCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCT                                                     >  SRR4302547.5360413/1‑94 (MQ=60)
                                         CCGAAAGGGCTACTGGAGCCGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAGCAACGCGGTCAACGTATTGCCAGCCGCAACCT                                                     >  SRR4302547.4235796/1‑94 (MQ=60)
                                          CGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTG                                                    <  SRR4302547.2167857/94‑1 (MQ=60)
                                           GAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGT                                                   >  SRR4302547.4255162/1‑94 (MQ=60)
                                            AAAGGGCTACTGGAGCTGTCCTTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTG                                                  >  SRR4302547.6598707/1‑94 (MQ=60)
                                            AAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTG                                                  <  SRR4302547.3224193/94‑1 (MQ=60)
                                             AAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGG                                                 >  SRR4302547.2022430/1‑94 (MQ=60)
                                             AAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGG                                                 >  SRR4302547.1723931/1‑94 (MQ=60)
                                               GGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGAT                                               <  SRR4302547.5074677/94‑1 (MQ=60)
                                               GGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGAT                                               <  SRR4302547.1419851/94‑1 (MQ=60)
                                               GGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGAT                                               <  SRR4302547.2228592/94‑1 (MQ=60)
                                                 GCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTT                                             <  SRR4302547.4757888/94‑1 (MQ=60)
                                                  CTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTC                                            >  SRR4302547.5040968/1‑94 (MQ=60)
                                                  CTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTC                                            >  SRR4302547.226455/1‑94 (MQ=60)
                                                   TACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCC                                           >  SRR4302547.1102220/1‑94 (MQ=60)
                                                     CTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGT                                         <  SRR4302547.4829385/94‑1 (MQ=60)
                                                      TGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTT                                        <  SRR4302547.5004367/94‑1 (MQ=60)
                                                       GGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTC                                       >  SRR4302547.4401191/1‑94 (MQ=60)
                                                        GAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCC                                      <  SRR4302547.3949407/94‑1 (MQ=60)
                                                          GCTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCT                                    <  SRR4302547.2629717/94‑1 (MQ=60)
                                                           CTGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTG                                   <  SRR4302547.3987368/94‑1 (MQ=60)
                                                           CTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTG                                   >  SRR4302547.5877089/1‑94 (MQ=60)
                                                            TGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGT                                  >  SRR4302547.3628617/1‑94 (MQ=60)
                                                            TGTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGT                                  <  SRR4302547.2830650/94‑1 (MQ=60)
                                                             GTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTC                                 >  SRR4302547.2785213/1‑94 (MQ=60)
                                                             GTCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTC                                 >  SRR4302547.2967665/1‑94 (MQ=60)
                                                             GTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTC                                 <  SRR4302547.1192119/94‑1 (MQ=60)
                                                              TCATTACAGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCT                                >  SRR4302547.2595620/1‑94 (MQ=60)
                                                                     AGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTG                         >  SRR4302547.6262412/1‑94 (MQ=60)
                                                                     AGATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTG                         <  SRR4302547.6273824/94‑1 (MQ=60)
                                                                      GATTGGCGACCGGGAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGG                        >  SRR4302547.4467447/1‑94 (MQ=60)
                                                                      GATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGG                        <  SRR4302547.2788721/94‑1 (MQ=60)
                                                                      GATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGG                        <  SRR4302547.6690839/94‑1 (MQ=60)
                                                                       ATTGGCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGC                       <  SRR4302547.2332163/94‑1 (MQ=60)
                                                                         TGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGT                     >  SRR4302547.2018947/1‑94 (MQ=60)
                                                                           GCGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTT                   <  SRR4302547.483167/94‑1 (MQ=60)
                                                                            CGACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTT                  >  SRR4302547.3572049/1‑94 (MQ=60)
                                                                             GACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTG                 <  SRR4302547.6076279/94‑1 (MQ=60)
                                                                             GACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTG                 <  SRR4302547.5782925/94‑1 (MQ=60)
                                                                             GACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTG                 >  SRR4302547.1367308/1‑94 (MQ=60)
                                                                              ACCGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGC                >  SRR4302547.2596061/1‑94 (MQ=60)
                                                                                CGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGT              <  SRR4302547.5422004/94‑1 (MQ=60)
                                                                                CGGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGT              <  SRR4302547.4916951/94‑1 (MQ=60)
                                                                                 GGAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTA             >  SRR4302547.4982614/1‑94 (MQ=60)
                                                                                  GAAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTAT            <  SRR4302547.3030643/94‑1 (MQ=60)
                                                                                   AAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATG           >  SRR4302547.3391005/1‑94 (MQ=60)
                                                                                   AAGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATG           >  SRR4302547.5906616/1‑94 (MQ=60)
                                                                                    AGATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGG          >  SRR4302547.5998984/1‑94 (MQ=60)
                                                                                     GATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGA         >  SRR4302547.5694645/1‑94 (MQ=60)
                                                                                      ATCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGAT        <  SRR4302547.104368/94‑1 (MQ=60)
                                                                                       TCCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATG       <  SRR4302547.4342246/94‑1 (MQ=60)
                                                                                       TCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATG       <  SRR4302547.5323938/94‑1 (MQ=60)
                                                                                        CCTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGACGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGT      >  SRR4302547.2448794/1‑94 (MQ=60)
                                                                                         CTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTT     >  SRR4302547.247696/1‑94 (MQ=60)
                                                                                         CTGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTT     >  SRR4302547.1252487/1‑94 (MQ=60)
                                                                                          TGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTG    >  SRR4302547.2487550/1‑94 (MQ=60)
                                                                                          TGTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTG    >  SRR4302547.5116340/1‑94 (MQ=60)
                                                                                           GTGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGT   <  SRR4302547.5081127/94‑1 (MQ=60)
                                                                                            TGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTT  <  SRR4302547.3991887/94‑1 (MQ=60)
                                                                                            TGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTT  <  SRR4302547.4932057/94‑1 (MQ=60)
                                                                                            CGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTT  <  SRR4302547.2312707/94‑1 (MQ=60)
                                                                                            |                                                                                             
GAAACGAGTATCAGAGGTGTCTATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTT  >  NC_000913/1277158‑1277343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑