Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,286,275	A→G	intergenic (+68/+35)	b1228 → / ← tpr	hypothetical protein/protamine‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,275	0	A	G	87.9%	548.0 / 55.8	207	intergenic (+68/+35)	b1228/tpr	hypothetical protein/protamine‑like protein
Reads supporting (aligned to +/- strand): ref base A (13/12); new base G (100/82); total (113/94)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 211 total aligned reads displayed.

CTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCT  >  NC_000913/1286185‑1286364

cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACt                                                                                         >  1:6625360/1‑93 (MQ=255)

cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAAAGGTCCGGGACt                                                                                         >  2:6348004/1‑93 (MQ=255)

 tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCtt                                                                                        <  2:1665561/93‑1 (MQ=255)

  gACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                                                       <  2:2855242/93‑1 (MQ=255)

   gcGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCtttt                                                                                      <  1:5769345/92‑1 (MQ=255)

    cGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                                                                     >  1:5991266/1‑93 (MQ=255)

     gCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCATAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGa                                                                                    >  2:3803844/1‑93 (MQ=255)

     gCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGCGTTCAGGGGCTTTTGa                                                                                    >  1:5597252/1‑93 (MQ=255)

      cTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGaa                                                                                   <  2:2286891/93‑1 (MQ=255)

        cGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAg                                                                                 >  2:6051775/1‑93 (MQ=255)

        cGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAg                                                                                 >  2:5114532/1‑93 (MQ=255)

            ccGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAt                                                                             <  1:975440/93‑1 (MQ=255)

            ccGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAt                                                                             <  2:6357529/93‑1 (MQ=255)

             cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGTTTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtg                                                                            >  2:1327823/1‑93 (MQ=255)

             cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtg                                                                            <  1:638426/93‑1 (MQ=255)

             cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtg                                                                            <  1:4555041/93‑1 (MQ=255)

             cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtg                                                                            <  1:4130397/93‑1 (MQ=255)

             cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtg                                                                            <  1:2224826/93‑1 (MQ=255)

             cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtg                                                                            >  1:1025379/1‑93 (MQ=255)

              gAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtgg                                                                           >  2:1524875/1‑93 (MQ=255)

              gAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtgg                                                                           >  2:3957366/1‑93 (MQ=255)

              gAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtgg                                                                           >  2:4644517/1‑93 (MQ=255)

               aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtggt                                                                          <  2:1466818/93‑1 (MQ=255)

               aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtggt                                                                          <  2:6139818/93‑1 (MQ=255)

               aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggt                                                                          >  2:743227/1‑93 (MQ=255)

               aaCCTTAGTCGAAGCTTATCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGCGAGTTCAGGGGCTTTTGGAAGTGAtggg                                                                          >  1:1075473/1‑92 (MQ=14)

                 ccTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtggtgg                                                                        >  2:4856390/1‑93 (MQ=255)

                  cTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtggtggt                                                                       <  2:5450260/93‑1 (MQ=255)

                   ttAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtggtggtg                                                                      >  2:233942/1‑93 (MQ=255)

                    tAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAtggtggtgg                                                                     >  1:1854822/1‑93 (MQ=255)

                     aGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTggg                                                                    >  2:2998681/1‑93 (MQ=255)

                      gTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTgggg                                                                   >  2:4914738/1‑93 (MQ=255)

                      gTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTgggg                                                                   <  1:5056267/93‑1 (MQ=255)

                          aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAg                                                               <  2:5367887/93‑1 (MQ=255)

                          aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAg                                                               <  1:5810575/93‑1 (MQ=255)

                          aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAg                                                               >  1:191199/1‑93 (MQ=255)

                            gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                             <  2:5780693/93‑1 (MQ=255)

                            gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                             >  1:6584573/1‑93 (MQ=255)

                            gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                             <  1:2323673/93‑1 (MQ=255)

                             cTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                                            >  1:6330432/1‑93 (MQ=255)

                              ttCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                                           >  1:6218981/1‑93 (MQ=255)

                              ttCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                                           >  1:2901498/1‑93 (MQ=255)

                                ctcATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAc                                                         >  2:1736035/1‑93 (MQ=255)

                                 tcATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                                                        >  1:3881298/1‑93 (MQ=255)

                                  cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc                                                       >  1:5452022/1‑93 (MQ=255)

                                  cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc                                                       <  2:6314152/93‑1 (MQ=255)

                                  cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc                                                       <  2:638534/93‑1 (MQ=255)

                                   aTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                                                      >  2:2485332/1‑93 (MQ=255)

                                   aTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                                                      <  1:819302/93‑1 (MQ=255)

                                   aTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                                                      >  1:1669689/1‑93 (MQ=255)

                                    tCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAGTACTCAg                                                     >  1:3271959/1‑93 (MQ=255)

                                       ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                                  <  2:4298402/93‑1 (MQ=255)

                                       ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                                  >  2:3236703/1‑93 (MQ=255)

                                          cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                                               <  2:1049179/93‑1 (MQ=255)

                                          cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                                               >  2:1753355/1‑93 (MQ=255)

                                          cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                                               <  2:3042121/93‑1 (MQ=255)

                                          cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                                               >  2:5862593/1‑93 (MQ=255)

                                           ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcg                                              >  2:5534235/1‑93 (MQ=255)

                                             gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                            >  1:1631769/1‑93 (MQ=255)

                                             gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                            <  2:3217951/93‑1 (MQ=255)

                                             gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                            >  1:2866380/1‑93 (MQ=255)

                                              cATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGATAGTGATGGAGGTGGGGGAATGATTACTCATCGCTgcgcgc                                           >  2:6651856/1‑93 (MQ=14)

                                               aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt                                          >  1:249481/1‑93 (MQ=255)

                                                  ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGGAGGATTACTCAGCGCTGCGCGCTTCg                                       >  1:4859544/1‑93 (MQ=255)

                                                   gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc                                      <  1:162010/93‑1 (MQ=37)

                                                     aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGccc                                    <  2:2015476/93‑1 (MQ=255)

                                                      taaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTCTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                                   <  1:2743797/92‑1 (MQ=255)

                                                      gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                                   >  2:565100/1‑93 (MQ=255)

                                                      gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                                   >  1:4662257/1‑93 (MQ=255)

                                                      gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTAGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                                   <  1:5902288/93‑1 (MQ=255)

                                                       aaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                  >  1:4002503/1‑93 (MQ=255)

                                                       aaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                  <  1:1243786/93‑1 (MQ=37)

                                                        atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                 >  1:3025358/1‑93 (MQ=255)

                                                        atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                 <  2:1761602/93‑1 (MQ=37)

                                                         tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg                                <  1:3542009/93‑1 (MQ=37)

                                                         tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg                                >  1:1435863/1‑93 (MQ=255)

                                                         tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg                                >  2:5333492/1‑93 (MQ=255)

                                                         tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg                                <  2:3112516/93‑1 (MQ=37)

                                                          atTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg                               >  1:2364475/1‑93 (MQ=255)

                                                           tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg                              >  1:5875345/1‑93 (MQ=255)

                                                            ttGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGAGGTGGGGGAAGGATTACTCAACGCTGCGCGATTCGCACTTCGGGt                             >  2:2650645/1‑93 (MQ=11)

                                                             tGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                            <  1:3938567/93‑1 (MQ=37)

                                                               aTTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGt                          >  2:3659540/1‑93 (MQ=255)

                                                               aTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGt                          <  2:6478717/93‑1 (MQ=255)

                                                               aTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGt                          <  2:213093/93‑1 (MQ=255)

                                                                   cGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc                      <  1:4329486/93‑1 (MQ=37)

                                                                    ggATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                                   <  1:5213092/79‑1 (MQ=255)

                                                                    ggATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                     >  2:5568670/1‑93 (MQ=25)

                                                                     gATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTg                    >  2:5687697/1‑93 (MQ=255)

                                                                         cGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGc                <  2:185615/93‑1 (MQ=37)

                                                                           cTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCaa              >  2:4503691/1‑93 (MQ=255)

                                                                           cTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa              >  1:2103413/1‑93 (MQ=21)

                                                                           cTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa              <  2:2064919/93‑1 (MQ=21)

                                                                             tGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACg            >  1:5342881/1‑93 (MQ=18)

                                                                              gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGc           <  2:5991266/93‑1 (MQ=40)

                                                                               agagTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGt                             <  2:626279/74‑1 (MQ=21)

                                                                               agagTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGct          >  1:376997/1‑93 (MQ=21)

                                                                                 agTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                  <  2:1199734/67‑1 (MQ=18)

                                                                                  gTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctctc       <  2:6596518/93‑1 (MQ=14)

                                                                                  gTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTAGTTGCCTGCGGCAACGctctc       >  2:1947046/1‑93 (MQ=9)

CTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCT  >  NC_000913/1286185‑1286364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGC  >  NC_000913/1286182‑1286367

tgcCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGG                                                                                              <  SRR4302547.29220/91‑1 (MQ=60)

TCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGG                                                                                              >  SRR4302547.3114137/1‑94 (MQ=60)

 CGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGC                                                                                             <  SRR4302547.2024321/94‑1 (MQ=60)

  GCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACT                                                                                            >  SRR4302547.6763281/1‑94 (MQ=60)

    TGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGGCTTT                                                                                          >  SRR4302547.1785361/1‑94 (MQ=60)

     GACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTT                                                                                         <  SRR4302547.165320/94‑1 (MQ=60)

      GCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTG                                                                                        <  SRR4302547.5888670/94‑1 (MQ=60)

      ACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTG                                                                                        >  SRR4302547.6115572/1‑94 (MQ=60)

       CGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGCGTTCAGGGGCTTTTGA                                                                                       >  SRR4302547.5712718/1‑94 (MQ=60)

       CGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGA                                                                                       >  SRR4302547.6292152/1‑94 (MQ=60)

       CGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGA                                                                                       <  SRR4302547.6407889/94‑1 (MQ=60)

        GCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGCGTTCAGGGGCTTTTGAA                                                                                      >  SRR4302547.3173243/1‑94 (MQ=60)

         CTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAA                                                                                     >  SRR4302547.2469010/1‑94 (MQ=60)

         CTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAA                                                                                     >  SRR4302547.6354564/1‑94 (MQ=60)

           CGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGT                                                                                   <  SRR4302547.6727190/94‑1 (MQ=60)

            GAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTG                                                                                  <  SRR4302547.1151684/94‑1 (MQ=60)

            GAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTG                                                                                  <  SRR4302547.6282270/94‑1 (MQ=60)

            GAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTG                                                                                  <  SRR4302547.2332072/94‑1 (MQ=60)

            GAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTG                                                                                  >  SRR4302547.5342628/1‑94 (MQ=60)

             AGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGA                                                                                 <  SRR4302547.2819991/94‑1 (MQ=58)

             AGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                                                                                 >  SRR4302547.5490178/1‑94 (MQ=60)

              GCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGAT                                                                                <  SRR4302547.2737181/94‑1 (MQ=55)

               CCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATG                                                                               <  SRR4302547.5138904/94‑1 (MQ=53)

               CCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATG                                                                               >  SRR4302547.4992738/1‑94 (MQ=53)

               CCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATG                                                                               >  SRR4302547.1045511/1‑94 (MQ=53)

               CCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATG                                                                               <  SRR4302547.994408/94‑1 (MQ=53)

                CGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGG                                                                              <  SRR4302547.2269654/94‑1 (MQ=50)

                CGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGG                                                                              <  SRR4302547.4214422/94‑1 (MQ=50)

                CGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGG                                                                              <  SRR4302547.6190318/94‑1 (MQ=50)

                CGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGG                                                                              <  SRR4302547.650210/94‑1 (MQ=50)

                CGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGG                                                                              <  SRR4302547.4649268/94‑1 (MQ=50)

                 GAACCTTAGTCGAAGCTTATCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGCGAGTTCAGGGGCTTTTGGAAGTGATGGG                                                                             >  SRR4302547.1096936/1‑94 (MQ=44)

                  AACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTG                                                                            >  SRR4302547.3859993/1‑94 (MQ=50)

                  AACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTG                                                                            <  SRR4302547.6009472/94‑1 (MQ=50)

                    CCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGT                                                                          >  SRR4302547.5881629/1‑94 (MQ=50)

                     CTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTG                                                                         >  SRR4302547.2559654/1‑94 (MQ=48)

                     CTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTG                                                                         <  SRR4302547.6484387/94‑1 (MQ=48)

                      TTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGG                                                                        <  SRR4302547.1456584/94‑1 (MQ=45)

                      TTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGG                                                                        >  SRR4302547.1891473/1‑94 (MQ=45)

                       TAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGG                                                                       >  SRR4302547.4042600/1‑94 (MQ=43)

                       TAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGG                                                                       <  SRR4302547.3699582/94‑1 (MQ=43)

                        AGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGG                                                                      <  SRR4302547.2942991/94‑1 (MQ=40)

                         GTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGG                                                                     <  SRR4302547.5160626/94‑1 (MQ=38)

                           CGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAA                                                                   >  SRR4302547.4711963/1‑94 (MQ=38)

                           CGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAA                                                                   >  SRR4302547.518434/1‑94 (MQ=38)

                            GAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAG                                                                  >  SRR4302547.194789/1‑94 (MQ=38)

                             AAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGG                                                                 <  SRR4302547.5930784/94‑1 (MQ=38)

                             AAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGG                                                                 <  SRR4302547.1951321/94‑1 (MQ=38)

                              AGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGA                                                                >  SRR4302547.6721677/1‑94 (MQ=38)

                               GCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                                               <  SRR4302547.2370769/94‑1 (MQ=38)

                               GCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                                               <  SRR4302547.5898939/94‑1 (MQ=38)

                               GCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                                               >  SRR4302547.6462152/1‑94 (MQ=38)

                                CTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                                              >  SRR4302547.6348132/1‑94 (MQ=38)

                                CTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                                              >  SRR4302547.2959355/1‑94 (MQ=38)

                                  TCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAC                                                            >  SRR4302547.5960221/1‑94 (MQ=38)

                                  TCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAC                                                            >  SRR4302547.2740668/1‑94 (MQ=38)

                                   CTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACT                                                           >  SRR4302547.3960260/1‑94 (MQ=38)

                                    TCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTC                                                          <  SRR4302547.5203518/94‑1 (MQ=38)

                                    TCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTC                                                          >  SRR4302547.5564681/1‑94 (MQ=38)

                                     CATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCA                                                         >  SRR4302547.1702588/1‑94 (MQ=60)

                                      ATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAG                                                        >  SRR4302547.2257003/1‑94 (MQ=38)

                                      ATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAG                                                        <  SRR4302547.834911/94‑1 (MQ=38)

                                      ATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGAGTACTCAG                                                        >  SRR4302547.3337480/1‑94 (MQ=36)

                                       TCCTTCCCCGTAGGGGCAGAATATTTGATTGCGTATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGC                                                       <  SRR4302547.4876361/94‑1 (MQ=29)

                                       TCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGC                                                       >  SRR4302547.4348566/1‑94 (MQ=38)

                                         CTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGC                                                     >  SRR4302547.1891638/1‑94 (MQ=38)

                                          TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCT                                                    <  SRR4302547.4628827/94‑1 (MQ=60)

                                             CCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCG                                                 >  SRR4302547.2166839/1‑94 (MQ=38)

                                               CGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCG                                               >  SRR4302547.1664069/1‑94 (MQ=35)

                                               CGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCG                                               <  SRR4302547.4684992/94‑1 (MQ=35)

                                               CGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCG                                               >  SRR4302547.2923637/1‑94 (MQ=60)

                                                 CATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCT                                             <  SRR4302547.1647753/94‑1 (MQ=30)

                                                 CATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCT                                             >  SRR4302547.254147/1‑94 (MQ=30)

                                                  ATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTT                                            >  SRR4302547.1053178/1‑94 (MQ=28)

                                                   TGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTC                                           <  SRR4302547.2242685/94‑1 (MQ=25)

                                                   TGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTC                                           <  SRR4302547.6568266/94‑1 (MQ=52)

                                                    GGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGGAGGATTACTCAGCGCTGCGCGCTTCG                                          >  SRR4302547.4960233/1‑94 (MQ=24)

                                                     GGCAGAATATTTGATTGCGTATTCGTTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGC                                         <  SRR4302547.5197001/94‑1 (MQ=0)

                                                      GCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCC                                        <  SRR4302547.164978/94‑1 (MQ=25)

                                                      GCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCC                                        <  SRR4302547.4025099/94‑1 (MQ=25)

                                                      GCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCC                                        >  SRR4302547.3960334/1‑94 (MQ=25)

                                                        AGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCT                                      >  SRR4302547.4758810/1‑94 (MQ=25)

                                                        AGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCT                                      <  SRR4302547.3029540/94‑1 (MQ=25)

                                                         TAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTCTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT                                     <  SRR4302547.2798829/94‑1 (MQ=24)

                                                         GAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT                                     <  SRR4302547.5320394/94‑1 (MQ=25)

                                                         GAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT                                     >  SRR4302547.4083863/1‑94 (MQ=25)

                                                         GAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTAGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT                                     <  SRR4302547.6024513/94‑1 (MQ=50)

                                                          AATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTC                                    <  SRR4302547.4787236/94‑1 (MQ=25)

                                                          AATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTC                                    <  SRR4302547.1268890/94‑1 (MQ=25)

                                                          AATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTC                                    >  SRR4302547.3085514/1‑94 (MQ=25)

                                                          AATATTTGATTGCGAAATAGCTTGAGAATTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTC                                    <  SRR4302547.6786238/94‑1 (MQ=22)

                                                           ATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG                                   <  SRR4302547.63419/94‑1 (MQ=25)

                                                           ATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG                                   <  SRR4302547.1678790/94‑1 (MQ=25)

                                                           ATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG                                   >  SRR4302547.1464569/1‑94 (MQ=25)

                                                           ATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG                                   >  SRR4302547.3564282/1‑94 (MQ=25)

                                                            TATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGG                                  >  SRR4302547.2412406/1‑94 (MQ=25)

                                                            TATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGG                                  <  SRR4302547.3614310/94‑1 (MQ=25)

                                                             TTTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGG                                 >  SRR4302547.5996972/1‑94 (MQ=52)

                                                             ATTTGATTGCGGATTCGGTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGG                                 <  SRR4302547.2949682/94‑1 (MQ=9)

                                                               TTGATTGCTGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTC                               <  SRR4302547.3940466/94‑1 (MQ=24)

                                                                TGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG                              >  SRR4302547.6019743/1‑94 (MQ=25)

                                                                TGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG                              <  SRR4302547.4018647/94‑1 (MQ=25)

                                                                 GATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGT                             >  SRR4302547.2406374/1‑94 (MQ=25)

                                                                  ATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTT                            <  SRR4302547.4141462/94‑1 (MQ=52)

                                                                   TTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTG                           <  SRR4302547.5652768/94‑1 (MQ=25)

                                                                     GCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCC                         >  SRR4302547.5878338/1‑94 (MQ=25)

                                                                     GCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCC                         <  SRR4302547.4188692/94‑1 (MQ=25)

                                                                      CGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCT                        <  SRR4302547.4418573/94‑1 (MQ=25)

                                                                         ATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCG                     <  SRR4302547.2245880/94‑1 (MQ=25)

                                                                         ATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGGCTGCG                     >  SRR4302547.3559951/1‑94 (MQ=23)

                                                                          TTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGG                    <  SRR4302547.3144069/94‑1 (MQ=52)

                                                                            CGCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCA                  >  SRR4302547.960939/1‑94 (MQ=20)

                                                                             GCTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAA                 >  SRR4302547.2145330/1‑94 (MQ=15)

                                                                               TTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACG               >  SRR4302547.5453031/1‑94 (MQ=10)

                                                                               TTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACG               <  SRR4302547.4069077/94‑1 (MQ=10)

                                                                                 GAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCT             <  SRR4302547.5174268/94‑1 (MQ=7)

                                                                                 GAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCT             <  SRR4302547.4351219/94‑1 (MQ=7)

                                                                                 GAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCT             <  SRR4302547.5915928/94‑1 (MQ=7)

                                                                                 GAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCT             >  SRR4302547.383984/1‑94 (MQ=7)

                                                                                     GTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCT         >  SRR4302547.4800526/1‑94 (MQ=0)

                                                                                      TTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTC        <  SRR4302547.2032893/94‑1 (MQ=5)

                                                                                            GGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGC  <  SRR4302547.816546/94‑1 (MQ=0)

TCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGC  >  NC_000913/1286182‑1286367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`