Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,398,212 A→G W17R (TGG→CGG)  ogt ← O‑6‑alkylguanine‑DNA/cysteine‑protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,398,2120AG88.5% 617.9 / 59.3 217W17R (TGG→CGG) ogtO‑6‑alkylguanine‑DNA/cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (7/18);  new base G (94/98);  total (101/116)
Fisher's exact test for biased strand distribution p-value = 5.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 224 total aligned reads displayed.

GATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATC  >  NC_000913/1398122‑1398304
                                                                                          |                                                                                            
gATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCcgca                                                                                            >  2:3831024/1‑93 (MQ=255)
  tAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGt                                                                                          <  2:1690950/93‑1 (MQ=255)
  tAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGt                                                                                          <  2:4645594/93‑1 (MQ=255)
    aTGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTgg                                                                                        <  2:1977501/93‑1 (MQ=255)
     tGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGa                                                                                       >  2:3019105/1‑93 (MQ=255)
      ggATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGAc                                                                                      >  1:2787924/1‑93 (MQ=255)
          gTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAg                                                                                  >  1:5187655/1‑93 (MQ=255)
          gTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAg                                                                                  <  2:5442365/93‑1 (MQ=255)
          gTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAg                                                                                  <  1:1427608/93‑1 (MQ=255)
           tCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgt                                                                                 >  1:5480981/1‑93 (MQ=255)
           tCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgt                                                                                 <  1:4864654/93‑1 (MQ=255)
           tCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgt                                                                                 <  2:2759091/93‑1 (MQ=255)
            ccAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAgtg                                                                                <  1:908867/93‑1 (MQ=255)
             cagcagCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtgg                                                                               <  1:5290106/93‑1 (MQ=255)
             cagcagCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtgg                                                                               <  2:4598708/93‑1 (MQ=255)
               gcagcTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtggcg                                                                             >  2:5160491/1‑93 (MQ=255)
               gcagcTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtggcg                                                                             <  1:4676452/93‑1 (MQ=255)
               gcagcTGCACCATGCGTTCCCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtggcg                                                                             >  1:6414871/1‑93 (MQ=255)
                cagcTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtggcgt                                                                            <  1:5609274/93‑1 (MQ=255)
                   cTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAgtggcgtggc                                                                         >  2:326003/1‑93 (MQ=255)
                      cACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAAt                                                                      <  1:4145750/93‑1 (MQ=255)
                       aCCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGCCAAtt                                                                     >  1:1027193/1‑93 (MQ=255)
                        ccATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAAttt                                                                    >  2:3080956/1‑93 (MQ=255)
                        ccATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAAttt                                                                    <  1:1695561/93‑1 (MQ=255)
                        ccATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAAttt                                                                    >  2:1235300/1‑93 (MQ=255)
                            gCGTTCGCTGTCCTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAAGCCCCCGCAGTCGACCCAGTGGCGTGGCAATTTTttc                                                                <  2:5400117/93‑1 (MQ=255)
                            gCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTttc                                                                <  1:2633056/93‑1 (MQ=255)
                            gCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTttc                                                                <  1:2320043/93‑1 (MQ=255)
                            gCGTTCGCTGTACCCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTttc                                                                >  1:6534654/1‑93 (MQ=255)
                              gTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTttctt                                                              >  2:3472765/1‑93 (MQ=255)
                                tCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCa                                                            <  2:3990408/93‑1 (MQ=255)
                                  gCTGTACTCTTCCCATTGAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAg                                                          <  2:6559395/93‑1 (MQ=255)
                                  gCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCGATTTTTTTTTCatt                                                          >  1:1683808/1‑91 (MQ=255)
                                  gCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAg                                                          <  1:3157627/93‑1 (MQ=255)
                                  gCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAg                                                          <  2:1467937/93‑1 (MQ=255)
                                   cTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGt                                                         <  1:5298855/93‑1 (MQ=255)
                                    tGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTa                                                        >  2:41801/1‑93 (MQ=255)
                                     gTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCCATTTTTTCTTCAAGTaa                                                       >  1:238652/1‑93 (MQ=255)
                                     gTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTaa                                                       >  1:4310130/1‑93 (MQ=255)
                                     gTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTaa                                                       <  1:41149/93‑1 (MQ=255)
                                      tACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAt                                                      >  1:6175509/1‑93 (MQ=255)
                                      tACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAt                                                      <  2:47160/93‑1 (MQ=255)
                                       aCTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAtc                                                     <  2:804867/93‑1 (MQ=255)
                                       aCTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAtc                                                     <  1:794115/93‑1 (MQ=255)
                                       aCTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAtc                                                     >  1:5001673/1‑93 (MQ=255)
                                         tctTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAtctc                                                   >  1:2916218/1‑93 (MQ=255)
                                         tctTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAtcta                                                   >  2:1356788/1‑92 (MQ=255)
                                          ctTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCa                                                  >  1:4467483/1‑93 (MQ=255)
                                           ttCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAg                                                 >  2:1935900/1‑93 (MQ=255)
                                            tCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCCATTTTTTCTTCAAGTAATCTTAGc                                                >  2:5679329/1‑93 (MQ=255)
                                            tCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGc                                                <  2:4503441/93‑1 (MQ=255)
                                             cccATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCa                                               <  2:3856900/93‑1 (MQ=255)
                                              ccATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCAt                                              <  2:4558901/93‑1 (MQ=255)
                                               cATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATc                                             >  1:3522152/1‑93 (MQ=255)
                                               cATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATc                                             >  1:3692317/1‑93 (MQ=255)
                                               cATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATc                                             <  1:840158/93‑1 (MQ=255)
                                                aTTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATcc                                            <  1:6015789/93‑1 (MQ=255)
                                                aTTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATcc                                            <  1:5841801/93‑1 (MQ=255)
                                                 ttCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATcct                                           >  2:4045846/1‑92 (MQ=255)
                                                  tCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGt                                          <  1:3130997/93‑1 (MQ=255)
                                                   cAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGtt                                         >  1:1819959/1‑93 (MQ=255)
                                                   cAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGtt                                         >  2:2075508/1‑93 (MQ=255)
                                                    aaCCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtc                                        <  2:6156073/93‑1 (MQ=255)
                                                     aCCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtct                                       <  2:4780573/93‑1 (MQ=255)
                                                     aCCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtct                                       <  2:2509352/93‑1 (MQ=255)
                                                     aCCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtct                                       <  2:6517602/93‑1 (MQ=255)
                                                        gcccgcAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtctctc                                    <  1:2058253/93‑1 (MQ=255)
                                                        gcccgcAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtctctc                                    >  2:1888785/1‑93 (MQ=255)
                                                        gcccgcAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTtctctc                                    <  1:4072496/93‑1 (MQ=255)
                                                          ccgcAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCtt                                  <  1:5923194/93‑1 (MQ=255)
                                                           cgcAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTa                                 <  1:1644899/93‑1 (MQ=255)
                                                              aGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGa                              <  2:3311407/93‑1 (MQ=255)
                                                              aGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGa                              >  2:1771649/1‑93 (MQ=255)
                                                                gCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACa                            <  1:1152345/93‑1 (MQ=255)
                                                                 cGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACaa                           >  1:5751401/1‑93 (MQ=255)
                                                                  gAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACaaa                          >  2:5253295/1‑93 (MQ=255)
                                                                  gAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACaaa                          <  2:2202698/93‑1 (MQ=255)
                                                                  gAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACaaa                          <  1:6488286/93‑1 (MQ=255)
                                                                   aaaTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAc                         <  1:3319770/93‑1 (MQ=255)
                                                                   aaaTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAc                         <  1:1866018/93‑1 (MQ=255)
                                                                   aaaTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAc                         >  1:4787847/1‑93 (MQ=255)
                                                                    aaTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACg                        >  1:5255543/1‑93 (MQ=255)
                                                                    aaTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACg                        >  1:1534190/1‑93 (MQ=255)
                                                                     aTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTTAGCATCCGTTCTCTCTTAAGACAAActg                       >  2:6388977/1‑91 (MQ=255)
                                                                     aTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTTAGACAAACGt                       >  1:2425241/1‑93 (MQ=255)
                                                                      ttGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGGGGGAATTTTTTATGTAATAACTCGCAGTCTACGTTCCCTCTTAAGACAAACgag                      >  2:3372526/1‑91 (MQ=255)
                                                                       tGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTgg                     <  2:6044243/93‑1 (MQ=255)
                                                                        gCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTggg                    <  1:2187567/93‑1 (MQ=255)
                                                                        gCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTggg                    <  2:6182397/93‑1 (MQ=255)
                                                                         cTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGt                   <  1:6270277/93‑1 (MQ=255)
                                                                         cTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGt                   <  1:5725863/93‑1 (MQ=255)
                                                                               gcgaaTCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATAc             <  1:135949/90‑1 (MQ=255)
                                                                               gCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATAc             >  2:5185938/1‑93 (MQ=255)
                                                                                cAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATAcc            >  2:2947170/1‑93 (MQ=255)
                                                                                 aaaTCGCCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATAccc           <  1:208246/93‑1 (MQ=255)
                                                                                 aaaTCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATAccc           >  1:3749553/1‑93 (MQ=255)
                                                                                 aaaTCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATAccc           <  2:2350360/93‑1 (MQ=255)
                                                                                    tCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGa        <  2:6495795/93‑1 (MQ=255)
                                                                                     cACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGAc       <  2:1014249/93‑1 (MQ=255)
                                                                                       cccGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCt     <  2:2497028/93‑1 (MQ=255)
                                                                                          |                                                                                            
GATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATC  >  NC_000913/1398122‑1398304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCG  >  NC_000913/1398119‑1398305
                                                                                             |                                                                                             
TGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCA                                                                                               <  SRR4302547.2540764/94‑1 (MQ=60)
  CGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCA                                                                                             >  SRR4302547.1582525/1‑94 (MQ=60)
    ATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGT                                                                                           <  SRR4302547.6653555/94‑1 (MQ=60)
       ATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGA                                                                                        <  SRR4302547.4803573/94‑1 (MQ=60)
        TGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGAC                                                                                       >  SRR4302547.2843760/1‑94 (MQ=60)
          GATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCC                                                                                     <  SRR4302547.5287307/94‑1 (MQ=60)
          GATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCC                                                                                     >  SRR4302547.2448523/1‑94 (MQ=60)
          GATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCC                                                                                     >  SRR4302547.3627191/1‑94 (MQ=60)
           ATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCA                                                                                    <  SRR4302547.4890109/94‑1 (MQ=60)
            TGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAG                                                                                   >  SRR4302547.5294416/1‑94 (MQ=60)
            TGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAG                                                                                   >  SRR4302547.5394268/1‑94 (MQ=60)
             GTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGT                                                                                  >  SRR4302547.5594158/1‑94 (MQ=60)
             GTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGT                                                                                  <  SRR4302547.6337352/94‑1 (MQ=60)
             GTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGT                                                                                  <  SRR4302547.1456168/94‑1 (MQ=60)
             GTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGT                                                                                  <  SRR4302547.2737328/94‑1 (MQ=60)
              TCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTG                                                                                 <  SRR4302547.4965463/94‑1 (MQ=60)
              TCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTG                                                                                 <  SRR4302547.5260380/94‑1 (MQ=60)
               CCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGG                                                                                <  SRR4302547.3491494/94‑1 (MQ=60)
               CCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGG                                                                                >  SRR4302547.3808287/1‑94 (MQ=60)
               CCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGG                                                                                <  SRR4302547.926379/94‑1 (MQ=60)
                CAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGC                                                                               <  SRR4302547.5399232/94‑1 (MQ=60)
                 AGCAGCTGCACCATGCGTTCCCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCG                                                                              >  SRR4302547.6548639/1‑94 (MQ=60)
                  GCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGT                                                                             <  SRR4302547.4773313/94‑1 (MQ=60)
                   CAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGGAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTG                                                                            <  SRR4302547.6310408/94‑1 (MQ=60)
                   CAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTG                                                                            <  SRR4302547.5725018/94‑1 (MQ=60)
                    AGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGG                                                                           >  SRR4302547.5103131/1‑94 (MQ=60)
                    AGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGG                                                                           <  SRR4302547.5852367/94‑1 (MQ=60)
                      CTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCA                                                                         <  SRR4302547.4026637/94‑1 (MQ=60)
                         CACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCTCAGGCGAGATTGCTCTTCGCAAATCACCAGCAGTGGACCCAGTGGCGTGGgaggt                                                                      >  SRR4302547.277666/1‑89 (MQ=60)
                         CACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATT                                                                      <  SRR4302547.4230146/94‑1 (MQ=60)
                         CACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGCCAATT                                                                      >  SRR4302547.1047374/1‑94 (MQ=60)
                           CCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAACTCACCCGCAGTGGACCCAGGGGCGTGGCAAGTTT                                                                    >  SRR4302547.171753/1‑94 (MQ=60)
                           CCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTT                                                                    >  SRR4302547.5961457/1‑94 (MQ=60)
                           CCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTT                                                                    <  SRR4302547.1728899/94‑1 (MQ=60)
                            CATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTT                                                                   >  SRR4302547.6103908/1‑94 (MQ=60)
                              TGCGTTCGCTGTACCCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTC                                                                 >  SRR4302547.6670654/1‑94 (MQ=60)
                               GCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCT                                                                <  SRR4302547.2367059/94‑1 (MQ=60)
                               GCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCT                                                                <  SRR4302547.2686076/94‑1 (MQ=60)
                                 GTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTC                                                              <  SRR4302547.6542025/94‑1 (MQ=60)
                                  TTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCA                                                             <  SRR4302547.4678520/94‑1 (MQ=60)
                                  TTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCA                                                             <  SRR4302547.5755322/94‑1 (MQ=60)
                                   TCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAA                                                            >  SRR4302547.2321270/1‑94 (MQ=60)
                                    CGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCGATTTTTTtttcatt                                                           >  SRR4302547.1716957/1‑87 (MQ=60)
                                    CGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAG                                                           >  SRR4302547.2374325/1‑94 (MQ=60)
                                     GCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGT                                                          <  SRR4302547.3220425/94‑1 (MQ=60)
                                      CTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTA                                                         >  SRR4302547.1464668/1‑94 (MQ=60)
                                      CTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTA                                                         <  SRR4302547.5408187/94‑1 (MQ=60)
                                       TGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCCATTTTTTCTTCAAGTAA                                                        >  SRR4302547.243121/1‑94 (MQ=60)
                                       TGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAA                                                        >  SRR4302547.4398793/1‑94 (MQ=60)
                                        GTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAT                                                       <  SRR4302547.41906/94‑1 (MQ=60)
                                        GTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAAT                                                       >  SRR4302547.6303691/1‑94 (MQ=60)
                                         TACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATC                                                      >  SRR4302547.5105070/1‑94 (MQ=60)
                                          ACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCT                                                     <  SRR4302547.809140/94‑1 (MQ=60)
                                           CTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTC                                                    <  SRR4302547.3242380/94‑1 (MQ=60)
                                           CTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTC                                                    >  SRR4302547.6760427/1‑94 (MQ=60)
                                           CTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTC                                                    >  SRR4302547.2974378/1‑94 (MQ=60)
                                            TCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCA                                                   >  SRR4302547.4559796/1‑94 (MQ=60)
                                             CTTCCCATTCAACCGCTCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAG                                                  <  SRR4302547.727254/94‑1 (MQ=60)
                                             CTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAG                                                  <  SRR4302547.598788/94‑1 (MQ=60)
                                                 CCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATC                                              >  SRR4302547.3594049/1‑94 (MQ=60)
                                                 CCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATC                                              >  SRR4302547.3767697/1‑94 (MQ=60)
                                                  CATTCAACCGCCCGCAGGCGAAATTGTTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCC                                             <  SRR4302547.5467306/94‑1 (MQ=60)
                                                  CATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCC                                             <  SRR4302547.856188/94‑1 (MQ=60)
                                                  CATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCC                                             >  SRR4302547.5924985/1‑94 (MQ=60)
                                                   ATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCG                                            <  SRR4302547.6140647/94‑1 (MQ=60)
                                                   ATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCG                                            <  SRR4302547.5962663/94‑1 (MQ=60)
                                                    TTCAACCGCCCGCAGGCGAACTTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGT                                           >  SRR4302547.6594967/1‑94 (MQ=60)
                                                     TCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTT                                          <  SRR4302547.3193138/94‑1 (MQ=60)
                                                     TCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTT                                          >  SRR4302547.1855935/1‑94 (MQ=60)
                                                      CAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTC                                         <  SRR4302547.2018395/94‑1 (MQ=60)
                                                        ACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTC                                       <  SRR4302547.996305/94‑1 (MQ=60)
                                                        ACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTC                                       >  SRR4302547.829314/1‑94 (MQ=60)
                                                        ACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTC                                       <  SRR4302547.5399988/94‑1 (MQ=60)
                                                        ACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTC                                       >  SRR4302547.1833198/1‑94 (MQ=60)
                                                           GCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCT                                    <  SRR4302547.2140601/94‑1 (MQ=60)
                                                           GCCCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCT                                    <  SRR4302547.2099324/94‑1 (MQ=60)
                                                           GCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCT                                    <  SRR4302547.4155227/94‑1 (MQ=60)
                                                             CCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTA                                  <  SRR4302547.6045956/94‑1 (MQ=60)
                                                             CCGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTA                                  <  SRR4302547.2114599/94‑1 (MQ=60)
                                                             CCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTA                                  <  SRR4302547.2337163/94‑1 (MQ=60)
                                                              CGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAA                                 >  SRR4302547.1857031/1‑94 (MQ=60)
                                                              CGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAA                                 >  SRR4302547.5326984/1‑94 (MQ=60)
                                                              CGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAA                                 <  SRR4302547.1677395/94‑1 (MQ=60)
                                                              CGCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAA                                 >  SRR4302547.5509735/1‑94 (MQ=60)
                                                               GCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAG                                >  SRR4302547.3318501/1‑94 (MQ=60)
                                                               GCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAG                                >  SRR4302547.4374978/1‑94 (MQ=60)
                                                               GCAGGCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAG                                <  SRR4302547.2492086/94‑1 (MQ=60)
                                                                   GCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAA                            >  SRR4302547.5870291/1‑94 (MQ=60)
                                                                   GCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAA                            <  SRR4302547.1175671/94‑1 (MQ=60)
                                                                   GCGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAA                            >  SRR4302547.2806229/1‑94 (MQ=60)
                                                                    CGAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAA                           >  SRR4302547.876914/1‑94 (MQ=60)
                                                                     GAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAC                          >  SRR4302547.1056797/1‑94 (MQ=60)
                                                                     GAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAC                          >  SRR4302547.4887048/1‑94 (MQ=60)
                                                                     GAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAC                          >  SRR4302547.162767/1‑94 (MQ=60)
                                                                     GAAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAC                          <  SRR4302547.448341/94‑1 (MQ=60)
                                                                     GAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAC                          <  SRR4302547.6623362/94‑1 (MQ=60)
                                                                      AAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACG                         <  SRR4302547.120613/94‑1 (MQ=60)
                                                                      AAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACG                         >  SRR4302547.5363745/1‑94 (MQ=60)
                                                                      AAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACG                         >  SRR4302547.1564726/1‑94 (MQ=60)
                                                                      AAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACG                         <  SRR4302547.1902869/94‑1 (MQ=60)
                                                                      AAATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACG                         <  SRR4302547.3386790/94‑1 (MQ=60)
                                                                       AATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTTAGACAAACGT                        >  SRR4302547.2474316/1‑94 (MQ=60)
                                                                       AATTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGT                        <  SRR4302547.1415728/94‑1 (MQ=60)
                                                                         TTGCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGG                      <  SRR4302547.4717788/94‑1 (MQ=60)
                                                                           GCTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGT                    <  SRR4302547.2231439/94‑1 (MQ=60)
                                                                            CTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTA                   <  SRR4302547.3105802/94‑1 (MQ=60)
                                                                            CTCATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTA                   <  SRR4302547.6400527/94‑1 (MQ=60)
                                                                            CTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTA                   >  SRR4302547.4208407/1‑94 (MQ=60)
                                                                            CTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTA                   <  SRR4302547.5844145/94‑1 (MQ=60)
                                                                              CATCGCAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAA                 >  SRR4302547.6167399/1‑94 (MQ=60)
                                                                                  GCGAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACC             <  SRR4302547.138404/94‑1 (MQ=60)
                                                                                  GCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACC             <  SRR4302547.5636870/94‑1 (MQ=60)
                                                                                   CAAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCC            >  SRR4302547.3826085/1‑94 (MQ=60)
                                                                                    AAATCGCCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCG           <  SRR4302547.212165/94‑1 (MQ=60)
                                                                                    AAATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCG           <  SRR4302547.2862563/94‑1 (MQ=60)
                                                                                     AATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAAAGTGGGTAAATACCCGG          >  SRR4302547.4158641/1‑94 (MQ=60)
                                                                                      ATCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGA         <  SRR4302547.3889094/94‑1 (MQ=60)
                                                                                       TCACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGAC        <  SRR4302547.3194292/94‑1 (MQ=60)
                                                                                         ACCCGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCT      <  SRR4302547.16978/94‑1 (MQ=60)
                                                                                            CGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATC   >  SRR4302547.3112183/1‑94 (MQ=60)
                                                                                            CGCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATC   <  SRR4302547.1315486/94‑1 (MQ=60)
                                                                                             GCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCG  <  SRR4302547.2082141/94‑1 (MQ=60)
                                                                                             GCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCG  <  SRR4302547.4368445/94‑1 (MQ=60)
                                                                                             GCAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCG  >  SRR4302547.1419536/1‑94 (MQ=60)
                                                                                             ACAGTGGACCCAGTGGCGTGGCAATTTTTTTTTCAAGGAATCTCAGCATACGTTATCTCTTAAGACAAACCTGGGTAAATACCCGGACCTATCG  >  SRR4302547.4785365/1‑94 (MQ=60)
                                                                                             ACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCG  <  SRR4302547.186599/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATTTTTTCTTCAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCG  >  NC_000913/1398119‑1398305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑