Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,438,614 A→G A65A (GCT→GCC)  ydbK ← putative oxidoreductase Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,438,6140AG89.6% 692.2 / 58.9 241A65A (GCT→GCC) ydbKputative oxidoreductase Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (11/14);  new base G (107/109);  total (118/123)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 250 total aligned reads displayed.

GTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCT  >  NC_000913/1438523‑1438705
                                                                                           |                                                                                           
gTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAg                                                                                            >  2:488260/1‑93 (MQ=255)
gTTTGTACAGCGTCGCGATCATCAGCAGCAACCCCTGCGATGACGTAACCGATGTTGAAAGGGCGCCCGTCTGCAAAGCGCCATGCACGGTAg                                                                                            <  1:6561632/93‑1 (MQ=255)
  ttGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCg                                                                                          <  1:5948298/93‑1 (MQ=255)
   tGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGa                                                                                         >  2:3700481/1‑93 (MQ=255)
   tGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGa                                                                                         <  1:1333678/93‑1 (MQ=255)
   tGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGCGGCGa                                                                                         >  1:3962088/1‑93 (MQ=255)
      aCAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAg                                                                                      >  1:2221719/1‑93 (MQ=255)
      aCAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAg                                                                                      <  1:3485991/93‑1 (MQ=255)
          cGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCAcc                                                                                  <  2:1769773/93‑1 (MQ=255)
           gTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCAccc                                                                                 >  2:4398688/1‑93 (MQ=255)
           gTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCAccc                                                                                 >  2:1949268/1‑93 (MQ=255)
            tCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCg                                                                                <  1:4272618/93‑1 (MQ=255)
            tCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCg                                                                                >  2:2625356/1‑93 (MQ=255)
             cGGGATCATAAGCAGCAAACCCTGCGATGACGGAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGc                                                                               <  2:1324554/93‑1 (MQ=255)
              gggATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCt                                                                              >  1:5625741/1‑93 (MQ=255)
               ggATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCtt                                                                             >  2:1609603/1‑93 (MQ=255)
                gATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTc                                                                            <  1:4184977/93‑1 (MQ=255)
                gATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTc                                                                            >  2:5763444/1‑93 (MQ=255)
                 atcatcAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTcc                                                                           >  2:1735364/1‑93 (MQ=255)
                 atcatcAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTcc                                                                           >  1:2585444/1‑93 (MQ=255)
                  tcatcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCg                                                                          <  1:292871/93‑1 (MQ=255)
                  tcatcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCg                                                                          >  2:5539128/1‑93 (MQ=255)
                    atcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGTGCCATGCACGGTGGCGATAGCACCCGCTTCCGAc                                                                        >  2:608005/1‑93 (MQ=255)
                    atcaGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGAc                                                                        >  2:705383/1‑93 (MQ=255)
                      cagcagCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTg                                                                      <  1:1626308/93‑1 (MQ=255)
                       agcagcAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGc                                                                     >  2:945566/1‑93 (MQ=255)
                        gcagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCa                                                                    >  2:5164692/1‑93 (MQ=255)
                         cagcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCAt                                                                   <  1:171257/93‑1 (MQ=255)
                           gcaAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCAttt                                                                 >  1:3902323/1‑93 (MQ=255)
                                cccTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAAcc                                                            >  1:110399/1‑93 (MQ=255)
                                 ccTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCa                                                           <  2:1006825/93‑1 (MQ=255)
                                  cTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc                                                          >  2:2950065/1‑93 (MQ=255)
                                  cTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc                                                          >  1:1399947/1‑93 (MQ=255)
                                  cTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc                                                          >  1:5796455/1‑93 (MQ=255)
                                  cTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc                                                          >  2:6467056/1‑93 (MQ=255)
                                  cTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAc                                                          <  2:6286139/93‑1 (MQ=255)
                                    gCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgc                                                        >  2:3378667/1‑93 (MQ=255)
                                    gCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgc                                                        >  2:6594582/1‑93 (MQ=255)
                                        tGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgtgg                                                    <  2:344969/93‑1 (MQ=255)
                                         gACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgtggt                                                   <  2:895652/93‑1 (MQ=255)
                                         gACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgtggt                                                   >  2:519611/1‑93 (MQ=255)
                                           cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgt                                                 >  1:1220712/1‑93 (MQ=255)
                                           cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgt                                                 <  2:753706/93‑1 (MQ=255)
                                           cGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgt                                                 >  2:2280671/1‑93 (MQ=255)
                                             tAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgtgt                                               >  1:5314184/1‑93 (MQ=255)
                                             tAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgtgt                                               >  1:1906448/1‑93 (MQ=255)
                                             tAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGgtgtgt                                               <  1:3423603/93‑1 (MQ=255)
                                              aaaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtc                                              >  2:3284349/1‑93 (MQ=255)
                                              aaaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtc                                              <  1:4407143/93‑1 (MQ=255)
                                               aaCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtct                                             >  1:1286344/1‑93 (MQ=255)
                                                aCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtctc                                            <  1:1563001/93‑1 (MQ=255)
                                                aCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtctc                                            <  1:4780036/93‑1 (MQ=255)
                                                aCGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGtctc                                            >  2:2756086/1‑93 (MQ=255)
                                                 cGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTcc                                           >  2:5077896/1‑93 (MQ=255)
                                                   aTGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTcccc                                         <  1:1852015/93‑1 (MQ=255)
                                                    tGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCa                                        >  1:4671534/1‑93 (MQ=255)
                                                     gTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCaa                                       <  1:3510778/93‑1 (MQ=255)
                                                     gTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCaa                                       <  2:4323622/93‑1 (MQ=255)
                                                      ttGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCaaa                                      <  1:2396581/93‑1 (MQ=255)
                                                          aaGGGCTCCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGtt                                  <  1:3145292/93‑1 (MQ=255)
                                                           aGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTc                                 <  2:3137144/93‑1 (MQ=255)
                                                            gggCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCt                                >  2:4640053/1‑93 (MQ=255)
                                                            gggCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCt                                <  1:5706655/93‑1 (MQ=255)
                                                             ggCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCtt                               >  2:4848613/1‑93 (MQ=255)
                                                             ggCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCtt                               <  2:2163203/93‑1 (MQ=255)
                                                              gCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCttt                              <  1:5445056/93‑1 (MQ=255)
                                                               cACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTa                             <  1:6629764/93‑1 (MQ=255)
                                                               cACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTa                             >  2:6272877/1‑93 (MQ=255)
                                                                aCCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTaa                            <  2:211061/93‑1 (MQ=255)
                                                                aCCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTaa                            >  2:2472910/1‑93 (MQ=255)
                                                                 cccGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTTTGTCTCCCCAAACGTTCTTTAAg                           >  2:1032905/1‑93 (MQ=255)
                                                                 cccGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAg                           >  1:3153137/1‑93 (MQ=255)
                                                                 cccGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAg                           <  1:2178529/93‑1 (MQ=255)
                                                                  ccGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGc                          <  1:5135594/93‑1 (MQ=255)
                                                                  ccGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGc                          >  2:1433000/1‑93 (MQ=255)
                                                                   cGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGcc                         <  1:5688989/93‑1 (MQ=255)
                                                                   cGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGcc                         <  1:2470868/93‑1 (MQ=255)
                                                                    gTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCg                        <  1:2630305/93‑1 (MQ=255)
                                                                     tCTGCAAAGCGCCATGCGCGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGt                       <  1:6362960/93‑1 (MQ=255)
                                                                     tCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGt                       >  1:300890/1‑93 (MQ=255)
                                                                      cTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGtt                      <  1:3731783/93‑1 (MQ=255)
                                                                       tGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGttt                     <  1:1042526/93‑1 (MQ=255)
                                                                       tGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGttt                     <  1:147794/93‑1 (MQ=255)
                                                                       tGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGttt                     <  1:1991896/93‑1 (MQ=255)
                                                                       tGCAAAGCGCCATGCACGATGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGttt                     >  1:622476/1‑93 (MQ=255)
                                                                        gCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTc                    >  1:4037997/1‑93 (MQ=255)
                                                                        gCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTc                    <  2:2847800/93‑1 (MQ=255)
                                                                         cAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTcc                   <  1:4427076/93‑1 (MQ=255)
                                                                           aaGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCgg                 <  1:3253009/93‑1 (MQ=255)
                                                                              cgcCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGccc              <  2:5700909/93‑1 (MQ=255)
                                                                              cgcCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGccc              <  1:5178733/93‑1 (MQ=255)
                                                                                ccATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAg            <  2:3801291/93‑1 (MQ=255)
                                                                                ccATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAg            <  2:5689087/93‑1 (MQ=255)
                                                                                 cATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAgg           >  2:5462105/1‑93 (MQ=255)
                                                                                  aTGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGc          <  1:702451/93‑1 (MQ=255)
                                                                                   tGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCa         <  1:631206/93‑1 (MQ=255)
                                                                                    gCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCAt        <  2:39/93‑1 (MQ=255)
                                                                                     cACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATc       >  1:201877/1‑93 (MQ=255)
                                                                                      aCGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCa      >  1:81190/1‑93 (MQ=255)
                                                                                        ggTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGAGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGc    >  2:3552229/1‑93 (MQ=255)
                                                                                           |                                                                                           
GTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCT  >  NC_000913/1438523‑1438705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTG  >  NC_000913/1438521‑1438706
                                                                                             |                                                                                            
CAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTG                                                                                              >  SRR4302547.5935545/1‑94 (MQ=60)
CAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTG                                                                                              <  SRR4302547.4369891/94‑1 (MQ=60)
  GTTTGTACAGCGTCGCGATCATCAGCAGCAACCCCTGCGATGACGTAACCGATGTTGAAAGGGCGCCCGTCTGCAAAGCGCCATGCACGGTAGC                                                                                            <  SRR4302547.6698267/94‑1 (MQ=60)
    TTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGA                                                                                          <  SRR4302547.6071642/94‑1 (MQ=60)
    TTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGA                                                                                          >  SRR4302547.956432/1‑94 (MQ=60)
    TTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGCGGCGA                                                                                          >  SRR4302547.4042687/1‑94 (MQ=60)
     TGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGAT                                                                                         <  SRR4302547.1360487/94‑1 (MQ=60)
     TGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGAT                                                                                         >  SRR4302547.2098277/1‑94 (MQ=60)
     TGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGAT                                                                                         <  SRR4302547.5135063/94‑1 (MQ=60)
      GTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATA                                                                                        <  SRR4302547.782710/94‑1 (MQ=60)
      GTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATA                                                                                        >  SRR4302547.5264017/1‑94 (MQ=60)
      GTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATA                                                                                        >  SRR4302547.4734919/1‑94 (MQ=60)
       TACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAG                                                                                       >  SRR4302547.2266480/1‑94 (MQ=60)
       TACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGAGAG                                                                                       >  SRR4302547.1325297/1‑94 (MQ=60)
        ACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGC                                                                                      >  SRR4302547.5379204/1‑94 (MQ=60)
        ACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGC                                                                                      <  SRR4302547.3557110/94‑1 (MQ=60)
         CAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCA                                                                                     <  SRR4302547.2353688/94‑1 (MQ=60)
         CAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCA                                                                                     <  SRR4302547.5588453/94‑1 (MQ=60)
          AGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCAC                                                                                    >  SRR4302547.980566/1‑94 (MQ=60)
          AGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCAC                                                                                    >  SRR4302547.3938007/1‑94 (MQ=60)
           GCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACC                                                                                   <  SRR4302547.4999729/94‑1 (MQ=60)
           GCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACC                                                                                   <  SRR4302547.3265531/94‑1 (MQ=60)
            CGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCC                                                                                  <  SRR4302547.4385791/94‑1 (MQ=60)
            CGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCC                                                                                  <  SRR4302547.2088125/94‑1 (MQ=60)
             GTCGGGATCCTCAGCAGCAATCCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCG                                                                                 >  SRR4302547.6639204/1‑94 (MQ=60)
             GTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCG                                                                                 <  SRR4302547.6740527/94‑1 (MQ=60)
             GTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCG                                                                                 <  SRR4302547.1904694/94‑1 (MQ=60)
              TCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGC                                                                                <  SRR4302547.4360321/94‑1 (MQ=60)
              TCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGC                                                                                >  SRR4302547.3541553/1‑94 (MQ=60)
               CGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCT                                                                               >  SRR4302547.5741838/1‑94 (MQ=60)
                GGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTT                                                                              >  SRR4302547.3570153/1‑94 (MQ=60)
                 GGATCATCAGCAGCAAACCCTGCGATGACGTAAACGGTGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGAGAGCACCCGCTTC                                                                             >  SRR4302547.5374482/1‑94 (MQ=60)
                  GATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCC                                                                            <  SRR4302547.4270278/94‑1 (MQ=60)
                  GATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCC                                                                            >  SRR4302547.2637611/1‑94 (MQ=60)
                  GATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCGGCTTCC                                                                            >  SRR4302547.5669229/1‑94 (MQ=60)
                   ATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCG                                                                           <  SRR4302547.619195/94‑1 (MQ=60)
                   ATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCG                                                                           >  SRR4302547.2222987/1‑94 (MQ=60)
                    TCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGA                                                                          <  SRR4302547.298313/94‑1 (MQ=60)
                     CATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGAC                                                                         >  SRR4302547.4529412/1‑94 (MQ=60)
                        CAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGC                                                                      <  SRR4302547.1658485/94‑1 (MQ=60)
                         AGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCA                                                                     <  SRR4302547.1223307/94‑1 (MQ=60)
                         AGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCA                                                                     >  SRR4302547.6044717/1‑94 (MQ=60)
                           CAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATT                                                                   <  SRR4302547.174392/94‑1 (MQ=60)
                            AGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTT                                                                  >  SRR4302547.3981681/1‑94 (MQ=60)
                             GCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTC                                                                 >  SRR4302547.1500160/1‑94 (MQ=60)
                             GCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTC                                                                 <  SRR4302547.4102403/94‑1 (MQ=60)
                               AAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAA                                                               <  SRR4302547.4019528/94‑1 (MQ=60)
                               AAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAA                                                               >  SRR4302547.1431555/1‑94 (MQ=60)
                               AAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAA                                                               >  SRR4302547.2879522/1‑94 (MQ=60)
                                 AGCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACC                                                             <  SRR4302547.679267/94‑1 (MQ=60)
                                 ACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACC                                                             >  SRR4302547.112415/1‑94 (MQ=60)
                                   CCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAC                                                           <  SRR4302547.57616/94‑1 (MQ=60)
                                   CCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAC                                                           >  SRR4302547.5430494/1‑94 (MQ=60)
                                   CCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAC                                                           >  SRR4302547.3175959/1‑94 (MQ=60)
                                   CCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAC                                                           <  SRR4302547.3636370/94‑1 (MQ=60)
                                   CCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAC                                                           >  SRR4302547.1427963/1‑94 (MQ=60)
                                   CCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCAC                                                           >  SRR4302547.5916356/1‑94 (MQ=60)
                                    CTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACG                                                          <  SRR4302547.299922/94‑1 (MQ=60)
                                     TGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGC                                                         <  SRR4302547.5854276/94‑1 (MQ=60)
                                       CGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGG                                                       >  SRR4302547.3212728/1‑94 (MQ=60)
                                         ATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCGTGCACGGTGGCGATAGCACGCGCTTCCGACTTCATTTCAACTACGCGAGG                                                     >  SRR4302547.4504406/1‑94 (MQ=60)
                                         ATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGG                                                     <  SRR4302547.2448042/94‑1 (MQ=60)
                                         ATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGG                                                     <  SRR4302547.3385383/94‑1 (MQ=60)
                                            ACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGT                                                  >  SRR4302547.1245348/1‑94 (MQ=60)
                                             CGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTG                                                 <  SRR4302547.3753547/94‑1 (MQ=60)
                                              GTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGT                                                >  SRR4302547.1734088/1‑94 (MQ=60)
                                              GTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGT                                                >  SRR4302547.1944175/1‑94 (MQ=60)
                                              GTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGT                                                >  SRR4302547.5423774/1‑94 (MQ=60)
                                              GTAAACGACGTGGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGGCTGCATTTCAACCGCGCGTGGTGTGT                                                <  SRR4302547.5867138/94‑1 (MQ=60)
                                               TAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTC                                               >  SRR4302547.5927958/1‑94 (MQ=60)
                                               TAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGCGTGTC                                               >  SRR4302547.3095144/1‑94 (MQ=60)
                                               TAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTC                                               <  SRR4302547.3493313/94‑1 (MQ=60)
                                                AAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCT                                              <  SRR4302547.4497964/94‑1 (MQ=60)
                                                AAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCT                                              >  SRR4302547.1312247/1‑94 (MQ=60)
                                                AAACGATGTAGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCT                                              <  SRR4302547.4418699/94‑1 (MQ=60)
                                                  ACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCC                                            <  SRR4302547.4879046/94‑1 (MQ=60)
                                                  ACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCC                                            <  SRR4302547.1594140/94‑1 (MQ=60)
                                                     ATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCA                                         <  SRR4302547.1888610/94‑1 (MQ=60)
                                                     ATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCA                                         >  SRR4302547.4768270/1‑94 (MQ=60)
                                                      TGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAA                                        >  SRR4302547.2830645/1‑94 (MQ=60)
                                                       GTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAA                                       <  SRR4302547.3582429/94‑1 (MQ=60)
                                                        TTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAAC                                      <  SRR4302547.256065/94‑1 (MQ=60)
                                                        TTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAAC                                      <  SRR4302547.2445131/94‑1 (MQ=60)
                                                         TGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACG                                     <  SRR4302547.2598235/94‑1 (MQ=60)
                                                           AAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTT                                   <  SRR4302547.1311053/94‑1 (MQ=60)
                                                            AAGGGCTCCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTC                                  <  SRR4302547.3207789/94‑1 (MQ=60)
                                                            AAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCGAAGGTTC                                  >  SRR4302547.2679124/1‑94 (MQ=60)
                                                            AAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTC                                  >  SRR4302547.158999/1‑94 (MQ=60)
                                                            AAGGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTC                                  <  SRR4302547.6429926/94‑1 (MQ=60)
                                                              GGGCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTT                                <  SRR4302547.5824540/94‑1 (MQ=60)
                                                                GCACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTA                              <  SRR4302547.5557561/94‑1 (MQ=60)
                                                                 CACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAA                             >  SRR4302547.1818853/1‑94 (MQ=60)
                                                                 CACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAA                             <  SRR4302547.1229824/94‑1 (MQ=60)
                                                                 CACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAA                             <  SRR4302547.6767774/94‑1 (MQ=60)
                                                                 CACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAA                             >  SRR4302547.4856757/1‑94 (MQ=60)
                                                                  ACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAG                            >  SRR4302547.3215818/1‑94 (MQ=60)
                                                                  ACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAG                            >  SRR4302547.694153/1‑94 (MQ=60)
                                                                  ACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAG                            <  SRR4302547.5814264/94‑1 (MQ=60)
                                                                  ACCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAG                            >  SRR4302547.1968136/1‑94 (MQ=60)
                                                                   CCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGC                           <  SRR4302547.4169976/94‑1 (MQ=60)
                                                                   CCCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGC                           >  SRR4302547.4013213/1‑94 (MQ=60)
                                                                   CCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGC                           <  SRR4302547.2222154/94‑1 (MQ=60)
                                                                    CCGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCC                          <  SRR4302547.5241401/94‑1 (MQ=60)
                                                                     CGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCG                         >  SRR4302547.6376421/1‑94 (MQ=60)
                                                                     CGTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCG                         <  SRR4302547.5806466/94‑1 (MQ=60)
                                                                     CGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCG                         <  SRR4302547.2520817/94‑1 (MQ=60)
                                                                      GTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGT                        <  SRR4302547.2683281/94‑1 (MQ=60)
                                                                      GTCTGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGT                        >  SRR4302547.306505/1‑94 (MQ=60)
                                                                       TCTGCAAAGCGCCATGCGCGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTT                       <  SRR4302547.6495699/94‑1 (MQ=60)
                                                                        CTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTT                      <  SRR4302547.3807978/94‑1 (MQ=60)
                                                                        CTGCAAAGCGCCATGCACGATGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTT                      >  SRR4302547.633979/1‑94 (MQ=60)
                                                                         TGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTC                     <  SRR4302547.150480/94‑1 (MQ=60)
                                                                         TGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTC                     >  SRR4302547.4119988/1‑94 (MQ=60)
                                                                         TGCAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTC                     <  SRR4302547.1063094/94‑1 (MQ=60)
                                                                         TGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTC                     <  SRR4302547.2031459/94‑1 (MQ=60)
                                                                          GCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCC                    <  SRR4302547.5821329/94‑1 (MQ=60)
                                                                           CAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCG                   <  SRR4302547.4518557/94‑1 (MQ=60)
                                                                           CAAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCG                   >  SRR4302547.4154962/1‑94 (MQ=60)
                                                                            AAAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGG                  <  SRR4302547.1376215/94‑1 (MQ=60)
                                                                             AAGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGC                 <  SRR4302547.3318004/94‑1 (MQ=60)
                                                                              AGCGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCC                <  SRR4302547.426498/94‑1 (MQ=60)
                                                                                CGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCTCA              >  SRR4302547.1610424/1‑94 (MQ=60)
                                                                                CGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCA              <  SRR4302547.5285330/94‑1 (MQ=60)
                                                                                CGCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCA              >  SRR4302547.6597067/1‑94 (MQ=60)
                                                                                 GCCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAG             >  SRR4302547.3225627/1‑94 (MQ=60)
                                                                                  CCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGG            <  SRR4302547.1081407/94‑1 (MQ=60)
                                                                                  CCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGG            >  SRR4302547.1641909/1‑94 (MQ=60)
                                                                                  CCATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGG            <  SRR4302547.1828132/94‑1 (MQ=60)
                                                                                    ATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCA          >  SRR4302547.1948796/1‑94 (MQ=60)
                                                                                    ATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCA          <  SRR4302547.715435/94‑1 (MQ=60)
                                                                                    ATGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCA          <  SRR4302547.2296566/94‑1 (MQ=60)
                                                                                     TGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCAT         <  SRR4302547.6477105/94‑1 (MQ=60)
                                                                                     TGCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCAT         <  SRR4302547.642870/94‑1 (MQ=60)
                                                                                      GCACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATC        >  SRR4302547.205650/1‑94 (MQ=60)
                                                                                       CACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCA       <  SRR4302547.1355704/94‑1 (MQ=60)
                                                                                       CACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCA       >  SRR4302547.82707/1‑94 (MQ=60)
                                                                                        ACGGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAG      >  SRR4302547.3497897/1‑94 (MQ=60)
                                                                                          GGTGGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCC    >  SRR4302547.5181421/1‑94 (MQ=60)
                                                                                            TAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTG  <  SRR4302547.2054883/94‑1 (MQ=60)
                                                                                             |                                                                                            
CAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGTGGTGTGTCTCCCCAAACGTTCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTG  >  NC_000913/1438521‑1438706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑