Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,488,295 G→A intergenic (+600/‑595) ald → / → cybB aldehyde dehydrogenase, NAD‑linked/cytochrome b(561)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,488,2950GA86.0% 703.8 / 76.0 250intergenic (+600/‑595)ald/cybBaldehyde dehydrogenase, NAD‑linked/cytochrome b(561)
Reads supporting (aligned to +/- strand):  ref base G (14/21);  new base A (102/113);  total (116/134)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 256 total aligned reads displayed.

AGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAA  >  NC_000913/1488203‑1488386
                                                                                            |                                                                                           
  gCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACAcc                                                                                           <  2:6617069/93‑1 (MQ=255)
   cATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCa                                                                                          >  2:3711688/1‑93 (MQ=255)
      gAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACgg                                                                                       <  1:5725118/93‑1 (MQ=255)
      gAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACgg                                                                                       >  2:4211980/1‑93 (MQ=255)
      gAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACgg                                                                                       >  2:5470292/1‑93 (MQ=255)
        aTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGaa                                                                                     <  2:506061/93‑1 (MQ=255)
          ggTCTTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAAc                                                                                   <  1:3359616/93‑1 (MQ=255)
             cgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAAt                                                                                <  2:2882504/93‑1 (MQ=255)
              gtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATg                                                                               <  2:80403/93‑1 (MQ=255)
              gtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATg                                                                               >  1:4872054/1‑93 (MQ=255)
              gtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATg                                                                               <  1:3311126/93‑1 (MQ=255)
                cATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGgt                                                                             >  2:5039308/1‑93 (MQ=255)
                   cGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTc                                                                          <  2:1666415/93‑1 (MQ=255)
                    gTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCg                                                                         <  2:6068712/93‑1 (MQ=255)
                     tGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGt                                                                        <  1:4056228/93‑1 (MQ=255)
                      gCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGtt                                                                       >  2:1210224/1‑93 (MQ=255)
                      gCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGtt                                                                       <  1:698791/93‑1 (MQ=255)
                        cGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGc                                                                     >  2:6034764/1‑93 (MQ=255)
                         gACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGcc                                                                    <  2:3485396/93‑1 (MQ=255)
                         gACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGcc                                                                    >  2:2149436/1‑93 (MQ=255)
                         gACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGcc                                                                    <  1:300037/93‑1 (MQ=255)
                         gACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGcc                                                                    >  1:3121551/1‑93 (MQ=255)
                          aCTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCa                                                                   <  2:5930679/93‑1 (MQ=255)
                          aCTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCa                                                                   >  1:3435576/1‑93 (MQ=255)
                          aCTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCa                                                                   <  2:2999941/93‑1 (MQ=255)
                          aCTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCa                                                                   >  1:4461450/1‑93 (MQ=255)
                            ttCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATc                                                                 >  2:3076867/1‑93 (MQ=255)
                              cTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCa                                                               <  1:6258548/93‑1 (MQ=255)
                              cTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCa                                                               >  1:1639928/1‑93 (MQ=255)
                               tATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCag                                                              >  2:6607732/1‑93 (MQ=255)
                                aTCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCaga                                                             <  2:328725/93‑1 (MQ=255)
                                 tCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCagag                                                            <  2:2764996/93‑1 (MQ=255)
                                 tCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCagag                                                            >  1:773856/1‑93 (MQ=255)
                                   ccGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAgtg                                                          <  1:878211/93‑1 (MQ=255)
                                     gAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtc                                                        <  2:4793366/93‑1 (MQ=255)
                                      aaaCTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtcg                                                       <  2:4943269/93‑1 (MQ=255)
                                      aaaCTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtcg                                                       >  1:1338737/1‑93 (MQ=255)
                                        aCTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtcgtc                                                     <  2:4902921/93‑1 (MQ=255)
                                        aCTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtcgtc                                                     <  2:534109/93‑1 (MQ=255)
                                        aCTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtcgtc                                                     <  1:5571405/93‑1 (MQ=255)
                                        aCTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTgtcgtc                                                     >  2:4839805/1‑93 (MQ=255)
                                           gTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCAtt                                                  >  2:4550400/1‑93 (MQ=255)
                                            tCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTg                                                 >  1:540787/1‑93 (MQ=255)
                                            tCATCCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTg                                                 <  1:2334222/93‑1 (MQ=255)
                                              aTGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGAc                                               >  1:3768352/1‑93 (MQ=255)
                                              aTGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGAc                                               <  1:1848092/93‑1 (MQ=255)
                                               tGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACg                                              <  1:3917707/93‑1 (MQ=255)
                                               tGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACg                                              <  2:1104394/93‑1 (MQ=255)
                                                gCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGt                                             <  2:5101125/93‑1 (MQ=255)
                                                  aaGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTta                                           <  1:2717203/93‑1 (MQ=255)
                                                   aGGCTTTGTCCATCGGCGCAAGACAGTTAGTGGTACATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTtat                                          <  2:899011/93‑1 (MQ=255)
                                                   aGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTtat                                          >  1:1030642/1‑93 (MQ=255)
                                                     gCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATaa                                        >  1:3838261/1‑93 (MQ=255)
                                                      cTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATaaa                                       <  2:2516914/93‑1 (MQ=255)
                                                         tGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGa                                    <  1:3581607/93‑1 (MQ=255)
                                                         tGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGa                                    <  2:3154969/93‑1 (MQ=255)
                                                            ccATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAg                                 >  2:1596057/1‑93 (MQ=255)
                                                             cATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGt                                <  2:635615/93‑1 (MQ=255)
                                                             cATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGt                                <  2:2947909/93‑1 (MQ=255)
                                                             cATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGt                                >  2:3967592/1‑93 (MQ=255)
                                                             cATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGt                                <  2:3949984/93‑1 (MQ=255)
                                                              aTCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGtt                               >  1:1127399/1‑93 (MQ=255)
                                                               tCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGttt                              <  1:4714833/93‑1 (MQ=255)
                                                               tCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGttt                              <  1:2562133/93‑1 (MQ=255)
                                                               tCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGttt                              <  2:4859171/93‑1 (MQ=255)
                                                                 ggCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTtttc                            >  2:2022803/1‑93 (MQ=255)
                                                                  gcgcAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTtttca                           <  2:1672617/93‑1 (MQ=255)
                                                                   cgcAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTtttcat                          <  2:2211497/93‑1 (MQ=255)
                                                                    gcAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTtttcatt                         <  2:2400419/93‑1 (MQ=255)
                                                                      aaGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTtttcatttc                       <  2:815929/93‑1 (MQ=255)
                                                                       aGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTtttcatttca                      <  1:714390/93‑1 (MQ=255)
                                                                        gACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAc                     >  1:4543563/1‑93 (MQ=255)
                                                                        gACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAc                     >  1:1938296/1‑93 (MQ=255)
                                                                         aCAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAcc                    <  1:2425320/93‑1 (MQ=255)
                                                                         aCAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAcc                    <  2:6264626/93‑1 (MQ=255)
                                                                         aCAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAAGATAGTTTTCATTTCAcc                    <  1:1443244/93‑1 (MQ=255)
                                                                          cAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCg                   <  1:2159091/93‑1 (MQ=255)
                                                                          cAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCg                   <  1:4662282/93‑1 (MQ=255)
                                                                             ttAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCa                <  1:6044294/93‑1 (MQ=255)
                                                                             ttAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCa                <  1:2211717/93‑1 (MQ=255)
                                                                              tAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAg               <  2:3613814/93‑1 (MQ=255)
                                                                              tAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAg               <  1:4899159/93‑1 (MQ=255)
                                                                                gtggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAggg             <  2:2033872/93‑1 (MQ=255)
                                                                                gtggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAggg             <  2:4412390/93‑1 (MQ=255)
                                                                                 tggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAggtg            >  1:4226217/1‑91 (MQ=255)
                                                                                 tggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAgggg            >  1:1717059/1‑93 (MQ=255)
                                                                                 tggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAgggg            >  2:5641902/1‑93 (MQ=255)
                                                                                  ggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGc           <  1:6439868/93‑1 (MQ=255)
                                                                                  ggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGc           <  2:4696642/93‑1 (MQ=255)
                                                                                  ggtgCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGc           <  1:5137679/93‑1 (MQ=255)
                                                                                      cATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGaa       >  1:5848150/1‑93 (MQ=255)
                                                                                        tGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAt     <  2:5814959/93‑1 (MQ=255)
                                                                                        tGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAt     >  1:5511593/1‑93 (MQ=255)
                                                                                        tGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAt     >  2:2895839/1‑93 (MQ=255)
                                                                                         gACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATc    >  1:595028/1‑93 (MQ=255)
                                                                                         gACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAta    >  2:5277576/1‑92 (MQ=255)
                                                                                         gACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATc    >  1:2021329/1‑93 (MQ=255)
                                                                                         gACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATc    >  2:602154/1‑93 (MQ=255)
                                                                                         gACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATc    <  2:3675154/93‑1 (MQ=255)
                                                                                           cGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCaa  >  1:4149663/1‑93 (MQ=255)
                                                                                            |                                                                                           
AGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAA  >  NC_000913/1488203‑1488386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAAC  >  NC_000913/1488202‑1488387
                                                                                             |                                                                                            
TAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACG                                                                                              >  SRR4302547.5557492/1‑94 (MQ=60)
TAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACA                                                                                              <  SRR4302547.6418887/94‑1 (MQ=60)
TAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACA                                                                                              >  SRR4302547.2601337/1‑94 (MQ=60)
  GGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACC                                                                                            <  SRR4302547.4616687/94‑1 (MQ=60)
    CATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCAC                                                                                          >  SRR4302547.3156327/1‑94 (MQ=60)
     ATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACG                                                                                         >  SRR4302547.4593983/1‑94 (MQ=60)
       GAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGA                                                                                       <  SRR4302547.5843387/94‑1 (MQ=60)
          TGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAAC                                                                                    >  SRR4302547.1091721/1‑94 (MQ=60)
           GGTCTTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACA                                                                                   <  SRR4302547.3427787/94‑1 (MQ=60)
            GTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAA                                                                                  >  SRR4302547.8387/1‑94 (MQ=60)
            GTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGATGCATGACACGACGGAAACAA                                                                                  >  SRR4302547.2054823/1‑94 (MQ=60)
             tcatcatattgccgcaTTCTATCCAGACACTGTCATGCAGGGCTTTGGCGATCGGCGCAAGACAGTTAGTGGTGCATGACACCAAGGAAACAAT                                                                                 <  SRR4302547.3521047/78‑1 (MQ=60)
              CGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATG                                                                                >  SRR4302547.914210/1‑94 (MQ=60)
              CGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATG                                                                                >  SRR4302547.4973016/1‑94 (MQ=60)
              CGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATG                                                                                >  SRR4302547.1338676/1‑94 (MQ=60)
              CGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATG                                                                                >  SRR4302547.1664379/1‑94 (MQ=60)
               GTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGG                                                                               >  SRR4302547.1887283/1‑94 (MQ=60)
               GTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGG                                                                               <  SRR4302547.3377887/94‑1 (MQ=60)
                TCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGT                                                                              >  SRR4302547.3733801/1‑94 (MQ=60)
                 CATCGTTCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTG                                                                             <  SRR4302547.3983410/94‑1 (MQ=60)
                 CATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTG                                                                             >  SRR4302547.6092060/1‑94 (MQ=60)
                  ATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGT                                                                            <  SRR4302547.6384222/94‑1 (MQ=60)
                  ATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGT                                                                            >  SRR4302547.267868/1‑94 (MQ=60)
                  ATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGT                                                                            >  SRR4302547.3481892/1‑94 (MQ=60)
                      TGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTT                                                                        <  SRR4302547.4138596/94‑1 (MQ=60)
                      TGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTT                                                                        >  SRR4302547.405221/1‑94 (MQ=60)
                      TGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTT                                                                        >  SRR4302547.3613431/1‑94 (MQ=60)
                       GCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTG                                                                       <  SRR4302547.711709/94‑1 (MQ=60)
                         CGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCC                                                                     >  SRR4302547.3183449/1‑94 (MQ=60)
                          GACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCA                                                                    >  SRR4302547.2560182/1‑94 (MQ=60)
                          GACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCA                                                                    <  SRR4302547.305634/94‑1 (MQ=60)
                          GACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCA                                                                    >  SRR4302547.3505617/1‑94 (MQ=60)
                          GACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCA                                                                    >  SRR4302547.4553655/1‑94 (MQ=60)
                           ACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCAT                                                                   >  SRR4302547.384080/1‑94 (MQ=60)
                            CTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATC                                                                  <  SRR4302547.5507067/94‑1 (MQ=60)
                            CTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATC                                                                  >  SRR4302547.713647/1‑94 (MQ=60)
                            CTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATC                                                                  >  SRR4302547.5465615/1‑94 (MQ=60)
                             TTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCC                                                                 >  SRR4302547.1773453/1‑94 (MQ=60)
                              TCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCA                                                                >  SRR4302547.1672343/1‑94 (MQ=60)
                              TCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCA                                                                <  SRR4302547.5998663/94‑1 (MQ=60)
                               CTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAG                                                               <  SRR4302547.6388562/94‑1 (MQ=60)
                               CTATCCCGAAAATGTCATGCACGGCGTTGGCCATCGGCGCCAGACAGTTAGTGGAGAATGACACCACGGAAACAATGGTGTCGTTGCCATCCAG                                                               >  SRR4302547.2215247/1‑94 (MQ=60)
                                TATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGA                                                              >  SRR4302547.3284063/1‑94 (MQ=60)
                                 ATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAG                                                             >  SRR4302547.788484/1‑94 (MQ=60)
                                    CCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGT                                                          <  SRR4302547.895087/94‑1 (MQ=60)
                                     CGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTG                                                         >  SRR4302547.1458935/1‑94 (MQ=60)
                                     CGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTC                                                         <  SRR4302547.2811158/94‑1 (MQ=60)
                                      GAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCG                                                        <  SRR4302547.6100056/94‑1 (MQ=60)
                                      GAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCG                                                        >  SRR4302547.1365642/1‑94 (MQ=60)
                                       AAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGT                                                       >  SRR4302547.3340011/1‑94 (MQ=60)
                                         ACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCA                                                     <  SRR4302547.5686290/94‑1 (MQ=60)
                                           TGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATT                                                   <  SRR4302547.6716000/94‑1 (MQ=60)
                                            GTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTG                                                  >  SRR4302547.550547/1‑94 (MQ=60)
                                            GTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTG                                                  <  SRR4302547.5921619/94‑1 (MQ=60)
                                             TCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGA                                                 <  SRR4302547.4224718/94‑1 (MQ=60)
                                             TCATCCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGA                                                 <  SRR4302547.2381528/94‑1 (MQ=60)
                                              CATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGAC                                                >  SRR4302547.3845207/1‑94 (MQ=60)
                                               ATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACG                                               >  SRR4302547.4916415/1‑94 (MQ=60)
                                               ATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACG                                               <  SRR4302547.1884624/94‑1 (MQ=60)
                                               ATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACG                                               <  SRR4302547.2174236/94‑1 (MQ=60)
                                                TGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGT                                              >  SRR4302547.4746552/1‑94 (MQ=60)
                                                TGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGT                                              <  SRR4302547.3997377/94‑1 (MQ=60)
                                                  CAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTA                                            >  SRR4302547.6750880/1‑94 (MQ=60)
                                                  CAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTA                                            >  SRR4302547.4475177/1‑94 (MQ=60)
                                                  CAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTA                                            >  SRR4302547.5654335/1‑94 (MQ=60)
                                                   AAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTAT                                           <  SRR4302547.2771751/94‑1 (MQ=60)
                                                   AAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTAT                                           >  SRR4302547.1050895/1‑94 (MQ=60)
                                                    AGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATA                                          <  SRR4302547.2785421/94‑1 (MQ=60)
                                                     GGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAA                                         >  SRR4302547.4177972/1‑94 (MQ=60)
                                                     GGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAA                                         >  SRR4302547.3916439/1‑94 (MQ=60)
                                                       CTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAC                                       >  SRR4302547.5682358/1‑94 (MQ=60)
                                                       CTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAC                                       <  SRR4302547.5880943/94‑1 (MQ=60)
                                                        TTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACG                                      <  SRR4302547.3170903/94‑1 (MQ=60)
                                                          TGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGAT                                    <  SRR4302547.3654684/94‑1 (MQ=60)
                                                          TGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGAT                                    <  SRR4302547.3418932/94‑1 (MQ=60)
                                                           GGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATA                                   <  SRR4302547.4987519/94‑1 (MQ=60)
                                                            GCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAG                                  <  SRR4302547.5070456/94‑1 (MQ=60)
                                                            GCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAG                                  <  SRR4302547.1595672/94‑1 (MQ=60)
                                                            GCCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAG                                  <  SRR4302547.3618993/94‑1 (MQ=60)
                                                             CCATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGT                                 >  SRR4302547.1303112/1‑94 (MQ=60)
                                                              CATCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTT                                >  SRR4302547.1150148/1‑94 (MQ=60)
                                                                TCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTT                              <  SRR4302547.4812443/94‑1 (MQ=60)
                                                                TCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTT                              <  SRR4302547.2051984/94‑1 (MQ=60)
                                                                TCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTT                              <  SRR4302547.4713778/94‑1 (MQ=60)
                                                                TCGGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTT                              <  SRR4302547.2613869/94‑1 (MQ=60)
                                                                  GGCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCA                            >  SRR4302547.734225/1‑94 (MQ=60)
                                                                   GCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCAT                           <  SRR4302547.1863798/94‑1 (MQ=60)
                                                                   GCGCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCAT                           <  SRR4302547.255162/94‑1 (MQ=60)
                                                                     GCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTT                         >  SRR4302547.4936286/1‑94 (MQ=60)
                                                                     GCAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTT                         <  SRR4302547.1673587/94‑1 (MQ=60)
                                                                      CAAGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTC                        <  SRR4302547.5143738/94‑1 (MQ=60)
                                                                        AGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAC                      >  SRR4302547.4637503/1‑94 (MQ=60)
                                                                        AGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAC                      >  SRR4302547.1976740/1‑94 (MQ=60)
                                                                        AGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAC                      <  SRR4302547.727609/94‑1 (MQ=60)
                                                                        AGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAC                      <  SRR4302547.2208510/94‑1 (MQ=60)
                                                                        AGACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCAC                      >  SRR4302547.2345198/1‑94 (MQ=60)
                                                                         GACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACC                     >  SRR4302547.4341171/1‑94 (MQ=60)
                                                                         GACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACC                     >  SRR4302547.579630/1‑94 (MQ=60)
                                                                          ACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCG                    <  SRR4302547.2474396/94‑1 (MQ=60)
                                                                          ACAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAAGATAGTTTTCATTTCACCG                    <  SRR4302547.1472110/94‑1 (MQ=60)
                                                                           CAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGG                   <  SRR4302547.2202243/94‑1 (MQ=60)
                                                                           CAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGG                   <  SRR4302547.4806144/94‑1 (MQ=60)
                                                                           CAGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGG                   <  SRR4302547.4758835/94‑1 (MQ=60)
                                                                            AGTTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGC                  >  SRR4302547.6445695/1‑94 (MQ=60)
                                                                              TTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAG                <  SRR4302547.2256197/94‑1 (MQ=60)
                                                                              TTAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAG                <  SRR4302547.6169702/94‑1 (MQ=60)
                                                                               TAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGG               <  SRR4302547.5000609/94‑1 (MQ=60)
                                                                               TAGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGG               <  SRR4302547.4297943/94‑1 (MQ=60)
                                                                                AGTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGG              <  SRR4302547.4032128/94‑1 (MQ=60)
                                                                                 GTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGTG             >  SRR4302547.4312578/1‑94 (MQ=60)
                                                                                 GTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGG             <  SRR4302547.2142412/94‑1 (MQ=60)
                                                                                 GTGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGG             >  SRR4302547.1750827/1‑94 (MQ=60)
                                                                                  TGGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGC            >  SRR4302547.2431175/1‑94 (MQ=60)
                                                                                   GGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCG           <  SRR4302547.6574070/94‑1 (MQ=60)
                                                                                   GGTGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCG           <  SRR4302547.5243524/94‑1 (MQ=60)
                                                                                     TGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGA         <  SRR4302547.639696/94‑1 (MQ=60)
                                                                                     TGCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGA         <  SRR4302547.2360804/94‑1 (MQ=60)
                                                                                      GCATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAA        >  SRR4302547.5969116/1‑94 (MQ=60)
                                                                                        ATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAT      >  SRR4302547.5625382/1‑94 (MQ=60)
                                                                                        ATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAT      >  SRR4302547.3858075/1‑94 (MQ=60)
                                                                                        ATGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAAT      <  SRR4302547.785089/94‑1 (MQ=60)
                                                                                         TGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATC     >  SRR4302547.605931/1‑94 (MQ=60)
                                                                                         TGACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATC     >  SRR4302547.2061569/1‑94 (MQ=60)
                                                                                          GACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCA    <  SRR4302547.3399189/94‑1 (MQ=60)
                                                                                          GACACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCA    >  SRR4302547.3495606/1‑94 (MQ=60)
                                                                                           ACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAA   >  SRR4302547.4234173/1‑94 (MQ=60)
                                                                                            CACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGTCGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAAC  <  SRR4302547.3409867/94‑1 (MQ=60)
                                                                                            CACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAAC  >  SRR4302547.5236457/1‑94 (MQ=60)
                                                                                            CACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAAC  >  SRR4302547.5324034/1‑94 (MQ=60)
                                                                                            CACCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAAC  >  SRR4302547.119873/1‑94 (MQ=60)
                                                                                             |                                                                                            
TAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAACGATAGTTTTCATTTCACCGGCAGGGGCGGAAATCAAC  >  NC_000913/1488202‑1488387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑