Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,595,883	A→G	T76T (ACT→ACC)	ydeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,595,883	0	A	G	90.0%	654.2 / 54.9	221	T76T (ACT→ACC)	ydeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (11/11); new base G (93/106); total (104/117)
Fisher's exact test for biased strand distribution p-value = 8.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 223 total aligned reads displayed.

AATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCT  >  NC_000913/1595793‑1595975

 aTAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTa                                                                                           >  1:3519796/1‑93 (MQ=255)

 aTAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTa                                                                                           <  2:5424467/93‑1 (MQ=255)

 aTAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTa                                                                                           <  2:1653455/93‑1 (MQ=255)

   aGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATc                                                                                         <  1:5627240/93‑1 (MQ=255)

    gTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCa                                                                                        >  2:3946786/1‑93 (MQ=255)

     tGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCaa                                                                                       <  2:3129911/93‑1 (MQ=255)

     tGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCaa                                                                                       <  2:2850038/93‑1 (MQ=255)

     tGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCaa                                                                                       <  1:3137192/93‑1 (MQ=255)

      ggTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAat                                                                                      >  1:4083766/1‑93 (MQ=255)

       gTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAata                                                                                     >  1:6553017/1‑93 (MQ=255)

       gTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAata                                                                                     <  1:745971/93‑1 (MQ=255)

       gTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAata                                                                                     >  1:1289162/1‑93 (MQ=255)

        ttAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAatat                                                                                    >  2:1283706/1‑93 (MQ=255)

         tAAGGAGGCATTACGGCCAGCCAAAATATTAAGCACTCCAGTGCCATACTAGCCAACCAGGTAGGCATCATAATCAACATCGGTATCAATAtt                                                                                   <  2:3778879/93‑1 (MQ=255)

         tAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATAtt                                                                                   <  2:4170834/93‑1 (MQ=255)

          aaGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTg                                                                                  <  1:5769606/93‑1 (MQ=255)

           aggaggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTgg                                                                                 >  2:4852365/1‑93 (MQ=255)

           aggaggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTgg                                                                                 >  1:214852/1‑93 (MQ=255)

           aggaggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTgg                                                                                 <  2:6497329/93‑1 (MQ=255)

             gaggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTa                                                                               >  2:1209197/1‑93 (MQ=255)

             gaggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTa                                                                               <  2:5615014/93‑1 (MQ=255)

              aggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTaa                                                                              <  1:163386/93‑1 (MQ=255)

               ggCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAAt                                                                             >  1:1570583/1‑93 (MQ=255)

                 cATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCt                                                                           >  2:2796857/1‑93 (MQ=255)

                 cATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCt                                                                           <  2:3348243/93‑1 (MQ=255)

                  aTTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTg                                                                          >  1:6297349/1‑93 (MQ=255)

                    tACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGAt                                                                        <  1:1784467/93‑1 (MQ=255)

                    tACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGAt                                                                        >  2:1429741/1‑93 (MQ=255)

                     aCCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATc                                                                       <  1:1032186/93‑1 (MQ=255)

                       cGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCAt                                                                     >  1:3154486/1‑93 (MQ=255)

                         ccagccaAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTa                                                                   <  2:4019837/93‑1 (MQ=255)

                           agccaAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATc                                                                 >  1:2039061/1‑93 (MQ=255)

                            gccaAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCa                                                                >  2:376112/1‑93 (MQ=255)

                            gccaAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCa                                                                <  1:1148747/93‑1 (MQ=255)

                            gccaAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCa                                                                >  2:2947677/1‑93 (MQ=255)

                              caAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAAc                                                              <  1:5572498/93‑1 (MQ=255)

                              caAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAAc                                                              >  2:4103717/1‑93 (MQ=255)

                               aaaaTATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACt                                                             <  1:5084169/93‑1 (MQ=255)

                                aaaTATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACtt                                                            <  2:5128939/93‑1 (MQ=255)

                                  atatTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTcc                                                          >  1:91388/1‑93 (MQ=255)

                                  atatTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTcc                                                          <  2:467446/93‑1 (MQ=255)

                                   tatTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCa                                                         >  1:2468422/1‑93 (MQ=255)

                                   tatTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCa                                                         >  2:2270779/1‑93 (MQ=255)

                                     ttAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGa                                                       <  2:2547021/93‑1 (MQ=255)

                                     ttAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGa                                                       <  2:1106020/93‑1 (MQ=255)

                                     ttAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGa                                                       >  1:1888653/1‑93 (MQ=255)

                                     ttAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGa                                                       >  1:3724314/1‑93 (MQ=255)

                                      tAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGAc                                                      <  1:4657233/93‑1 (MQ=255)

                                      tAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGAc                                                      >  2:6198163/1‑93 (MQ=255)

                                        aGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACtt                                                    <  2:855167/93‑1 (MQ=255)

                                          cACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGc                                                  <  2:2999828/93‑1 (MQ=255)

                                           ccTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCa                                                 <  1:3372639/92‑1 (MQ=255)

                                           aCTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCa                                                 >  2:3720209/1‑93 (MQ=255)

                                            cTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCAc                                                <  1:1937776/93‑1 (MQ=255)

                                             tCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCAcc                                               <  1:778711/93‑1 (MQ=255)

                                                aGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGa                                            <  2:747656/93‑1 (MQ=255)

                                                   gCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGc                                         <  2:4231264/93‑1 (MQ=255)

                                                    ccATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCt                                        >  2:5510934/1‑93 (MQ=255)

                                                    ccATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCt                                        <  2:3496949/93‑1 (MQ=255)

                                                      aTACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGa                                      >  1:6148909/1‑93 (MQ=255)

                                                      aTACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGa                                      >  1:4354411/1‑93 (MQ=255)

                                                       tACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGaa                                     >  1:4404719/1‑93 (MQ=255)

                                                        aCCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAAc                                    <  1:5647499/93‑1 (MQ=255)

                                                         ccAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAAcc                                   <  2:5295499/93‑1 (MQ=255)

                                                           aGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATAATGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGc                                 <  1:3526601/93‑1 (MQ=255)

                                                            gCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCt                                >  2:4497279/1‑93 (MQ=255)

                                                            gCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCt                                >  2:2181785/1‑93 (MQ=255)

                                                              cAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTTAACCGCTTa                              <  2:6645900/93‑1 (MQ=255)

                                                               aaCCAGGTAAGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAg                             >  2:4142944/1‑93 (MQ=255)

                                                                aCCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGt                            <  2:4939436/93‑1 (MQ=255)

                                                                aCCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGt                            >  1:2650023/1‑93 (MQ=255)

                                                                aCCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGCAATCTGATCATTATCAACTTCCAGACTTGCACCAGACTCTGAACCGCTTAGt                            >  2:1129912/1‑93 (MQ=255)

                                                                  cAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGc                          <  2:6538738/93‑1 (MQ=255)

                                                                   aGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGcc                         <  2:1479284/93‑1 (MQ=255)

                                                                    ggTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCCTAGTGCCa                        <  1:6485663/93‑1 (MQ=255)

                                                                     gTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAg                       <  1:3426164/93‑1 (MQ=255)

                                                                     gTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAg                       >  1:5398235/1‑93 (MQ=255)

                                                                       aGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCa                     >  1:5335180/1‑93 (MQ=255)

                                                                          catcatAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCaaaa                  >  1:1518440/1‑93 (MQ=255)

                                                                           atcatAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAac                 >  2:2017042/1‑93 (MQ=255)

                                                                           atcatAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAac                 >  1:3879547/1‑93 (MQ=255)

                                                                            tcatAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAaca                <  2:5064971/93‑1 (MQ=255)

                                                                            tcatAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAaca                <  1:5295645/93‑1 (MQ=255)

                                                                            tcatAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAaca                <  1:3954042/93‑1 (MQ=255)

                                                                             catAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAacac               >  2:2759778/1‑93 (MQ=255)

                                                                              atAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACAcc              <  1:1220718/93‑1 (MQ=255)

                                                                               tAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACAccc             <  1:1482718/93‑1 (MQ=255)

                                                                                  gCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGc          >  1:4918288/1‑93 (MQ=255)

                                                                                  gCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGc          <  2:2394602/93‑1 (MQ=255)

                                                                                  gCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGc          >  2:6027577/1‑93 (MQ=255)

                                                                                    aaCATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGt        <  1:411441/93‑1 (MQ=255)

                                                                                    aaCATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGt        <  2:6314218/93‑1 (MQ=255)

                                                                                    aaCATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGt        <  2:5213731/93‑1 (MQ=255)

                                                                                     aCATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTc       >  2:5846272/1‑93 (MQ=255)

                                                                                     aCATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTc       <  1:3715751/93‑1 (MQ=255)

                                                                                      cATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCa      <  1:2796996/93‑1 (MQ=255)

                                                                                       aTCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCaa     >  2:5659951/1‑93 (MQ=255)

                                                                                       aTCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCaa     <  1:3582824/93‑1 (MQ=255)

                                                                                          aGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCt  <  1:1234148/93‑1 (MQ=255)

                                                                                          aGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCt  <  1:2025097/93‑1 (MQ=255)

AATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCT  >  NC_000913/1595793‑1595975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGTAATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  >  NC_000913/1595790‑1595976

AGTAATAGTGGTTAAGGAGTCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCG                                                                                               <  SRR4302547.2966270/94‑1 (MQ=60)

AGTAATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCG                                                                                               <  SRR4302547.3060293/94‑1 (MQ=60)

AGTAATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCG                                                                                               <  SRR4302547.3484286/94‑1 (MQ=60)

 GTAATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGG                                                                                              <  SRR4302547.5855655/94‑1 (MQ=60)

   AATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTA                                                                                            >  SRR4302547.3591649/1‑94 (MQ=60)

      AGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCA                                                                                         <  SRR4302547.5743366/94‑1 (MQ=60)

      AGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCA                                                                                         >  SRR4302547.6283533/1‑94 (MQ=60)

        TGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAAT                                                                                       <  SRR4302547.3199501/94‑1 (MQ=60)

        TGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAAT                                                                                       >  SRR4302547.4166762/1‑94 (MQ=60)

         GGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATA                                                                                      >  SRR4302547.6689421/1‑94 (MQ=60)

         GGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATA                                                                                      >  SRR4302547.1315124/1‑94 (MQ=60)

          GTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATAT                                                                                     <  SRR4302547.760026/94‑1 (MQ=60)

          GTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATAT                                                                                     <  SRR4302547.3848012/94‑1 (MQ=60)

           TTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATT                                                                                    >  SRR4302547.3393711/1‑94 (MQ=60)

           TTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATT                                                                                    <  SRR4302547.775059/94‑1 (MQ=60)

           TTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATT                                                                                    <  SRR4302547.1897941/94‑1 (MQ=60)

           TTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATT                                                                                    <  SRR4302547.1672670/94‑1 (MQ=60)

             AAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGG                                                                                  <  SRR4302547.5888935/94‑1 (MQ=60)

             AAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGG                                                                                  >  SRR4302547.218905/1‑94 (MQ=60)

             AAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGG                                                                                  >  SRR4302547.2160705/1‑94 (MQ=60)

             AAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGG                                                                                  >  SRR4302547.3564417/1‑94 (MQ=60)

             AAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGG                                                                                  <  SRR4302547.5429776/94‑1 (MQ=60)

                 AGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAAT                                                                              <  SRR4302547.166382/94‑1 (MQ=60)

                 AGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAAT                                                                              >  SRR4302547.2075824/1‑94 (MQ=60)

                 AGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAAT                                                                              >  SRR4302547.359635/1‑94 (MQ=60)

                 AGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAAT                                                                              >  SRR4302547.1601848/1‑94 (MQ=60)

                    CATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTG                                                                           >  SRR4302547.6428253/1‑94 (MQ=60)

                    CATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTG                                                                           <  SRR4302547.3796282/94‑1 (MQ=60)

                     ATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGA                                                                          >  SRR4302547.3822223/1‑94 (MQ=60)

                     ATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGA                                                                          >  SRR4302547.1994168/1‑94 (MQ=60)

                       TACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATC                                                                        <  SRR4302547.1819810/94‑1 (MQ=60)

                        ACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCA                                                                       <  SRR4302547.5060386/94‑1 (MQ=60)

                        ACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCA                                                                       <  SRR4302547.1205329/94‑1 (MQ=60)

                        ACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCA                                                                       <  SRR4302547.1052480/94‑1 (MQ=60)

                         CCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCAT                                                                      <  SRR4302547.2082941/94‑1 (MQ=60)

                         CCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCAT                                                                      >  SRR4302547.3217208/1‑94 (MQ=60)

                          CGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATT                                                                     <  SRR4302547.3530497/94‑1 (MQ=60)

                          CGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATT                                                                     >  SRR4302547.5505923/1‑94 (MQ=60)

                          CGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATT                                                                     <  SRR4302547.311884/94‑1 (MQ=60)

                            CCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTAT                                                                   >  SRR4302547.6710022/1‑94 (MQ=60)

                             CAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATC                                                                  <  SRR4302547.1272212/94‑1 (MQ=60)

                             CAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATC                                                                  >  SRR4302547.1720458/1‑94 (MQ=60)

                             CAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATC                                                                  <  SRR4302547.3214154/94‑1 (MQ=60)

                             CAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATC                                                                  >  SRR4302547.2079727/1‑94 (MQ=60)

                               GCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAA                                                                <  SRR4302547.1171977/94‑1 (MQ=60)

                                CCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAct                                                               >  SRR4302547.5713140/1‑92 (MQ=60)

                                 CAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACT                                                              <  SRR4302547.5687405/94‑1 (MQ=60)

                                  AAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTT                                                             <  SRR4302547.2455599/94‑1 (MQ=60)

                                  AAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTT                                                             <  SRR4302547.5189053/94‑1 (MQ=60)

                                  AAAATATTAAGCACTCCAGTGCCATACCAGCAAACCAGGTAGGCATCATAAGCAGCATCGGTATCAATATTGGTAATCTGATCATTATCAACTT                                                             <  SRR4302547.5238317/94‑1 (MQ=60)

                                    AATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCC                                                           >  SRR4302547.93089/1‑94 (MQ=60)

                                     ATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCA                                                          >  SRR4302547.2518314/1‑94 (MQ=60)

                                     ATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCA                                                          <  SRR4302547.5983447/94‑1 (MQ=60)

                                      TATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAG                                                         <  SRR4302547.368699/94‑1 (MQ=60)

                                       ATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGA                                                        >  SRR4302547.3800343/1‑94 (MQ=60)

                                       ATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGA                                                        >  SRR4302547.1925979/1‑94 (MQ=60)

                                         TAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACT                                                      <  SRR4302547.4753686/94‑1 (MQ=60)

                                         TAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACT                                                      <  SRR4302547.4692133/94‑1 (MQ=60)

                                          AAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTT                                                     <  SRR4302547.4324497/94‑1 (MQ=60)

                                             CACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCC                                                  >  SRR4302547.4950358/1‑94 (MQ=60)

                                              CCTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCAC                                                 <  SRR4302547.3441143/94‑1 (MQ=60)

                                              ACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCAC                                                 >  SRR4302547.2579109/1‑94 (MQ=60)

                                               CTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACC                                                <  SRR4302547.1976205/94‑1 (MQ=60)

                                                TCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCA                                               >  SRR4302547.4488146/1‑94 (MQ=60)

                                                TCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCA                                               <  SRR4302547.793430/94‑1 (MQ=60)

                                                    GTGCCATACCAGCCAACCGGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACG                                           >  SRR4302547.3482797/1‑94 (MQ=60)

                                                    GTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACG                                           <  SRR4302547.791337/94‑1 (MQ=60)

                                                      GCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCT                                         <  SRR4302547.550292/94‑1 (MQ=60)

                                                        CATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGA                                       <  SRR4302547.4016623/94‑1 (MQ=60)

                                                        CATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGA                                       >  SRR4302547.6276481/1‑94 (MQ=60)

                                                        CATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGA                                       >  SRR4302547.4443864/1‑94 (MQ=60)

                                                        CATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGA                                       >  SRR4302547.1633792/1‑94 (MQ=60)

                                                         ATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAA                                      <  SRR4302547.51292/94‑1 (MQ=60)

                                                         ATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAA                                      <  SRR4302547.2904507/94‑1 (MQ=60)

                                                         ATACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAA                                      >  SRR4302547.4495459/1‑94 (MQ=60)

                                                           ACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACC                                    <  SRR4302547.5764067/94‑1 (MQ=60)

                                                           ACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACC                                    >  SRR4302547.270130/1‑94 (MQ=60)

                                                           ACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACC                                    >  SRR4302547.1966451/1‑94 (MQ=60)

                                                           ACCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACC                                    >  SRR4302547.5697945/1‑94 (MQ=60)

                                                            CCAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCG                                   >  SRR4302547.5778178/1‑94 (MQ=60)

                                                             CAGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGC                                  <  SRR4302547.815750/94‑1 (MQ=60)

                                                              AGCCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATAATGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCT                                 <  SRR4302547.3598609/94‑1 (MQ=60)

                                                                CCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTA                               <  SRR4302547.4377905/94‑1 (MQ=60)

                                                                CCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTA                               <  SRR4302547.1377472/94‑1 (MQ=60)

                                                                CCAACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTA                               <  SRR4302547.1246397/94‑1 (MQ=60)

                                                                  AACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGT                             >  SRR4302547.2703315/1‑94 (MQ=60)

                                                                  AACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGT                             >  SRR4302547.1347773/1‑94 (MQ=60)

                                                                   ACCAGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTG                            >  SRR4302547.15588/1‑94 (MQ=60)

                                                                     CAGGAAGGCATCATCAGCAACATCGGTATCATTCTTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACGGATTAGTGCC                          >  SRR4302547.2212333/1‑94 (MQ=60)

                                                                      AGGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCA                         >  SRR4302547.1545689/1‑94 (MQ=60)

                                                                       GGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAG                        >  SRR4302547.5509786/1‑94 (MQ=60)

                                                                       GGTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCCTAGTGCCAG                        <  SRR4302547.6620688/94‑1 (MQ=60)

                                                                        GTAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGC                       <  SRR4302547.3495946/94‑1 (MQ=60)

                                                                         TAGGCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCA                      >  SRR4302547.5445115/1‑94 (MQ=60)

                                                                         TAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCA                      >  SRR4302547.4172381/1‑94 (MQ=60)

                                                                            GCATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAA                   >  SRR4302547.1548684/1‑94 (MQ=60)

                                                                             CATCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAAC                  >  SRR4302547.3958485/1‑94 (MQ=60)

                                                                               TCATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACAC                <  SRR4302547.5404905/94‑1 (MQ=60)

                                                                               TCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACAC                <  SRR4302547.4034465/94‑1 (MQ=60)

                                                                                 ATAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCC              <  SRR4302547.1245354/94‑1 (MQ=60)

                                                                                  TAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCA             <  SRR4302547.5761920/94‑1 (MQ=60)

                                                                                  TAAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCA             <  SRR4302547.1512309/94‑1 (MQ=60)

                                                                                   AAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAG            <  SRR4302547.6453181/94‑1 (MQ=60)

                                                                                   AAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAG            >  SRR4302547.6279203/1‑94 (MQ=60)

                                                                                   AAGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCAGAACCGCTTAGTGCCAGCAAAACACCCAG            <  SRR4302547.6658015/94‑1 (MQ=60)

                                                                                    AGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGC           >  SRR4302547.1118903/1‑94 (MQ=60)

                                                                                    AGCAACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGC           >  SRR4302547.5020155/1‑94 (MQ=60)

                                                                                       AACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCaagtctggaagttgataat        <  SRR4302547.3951180/94‑20 (MQ=60)

                                                                                       AACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTC        <  SRR4302547.419074/94‑1 (MQ=60)

                                                                                       AACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTC        >  SRR4302547.358850/1‑94 (MQ=60)

                                                                                        ACATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCA       <  SRR4302547.3791593/94‑1 (MQ=60)

                                                                                         CATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAA      >  SRR4302547.4152543/1‑94 (MQ=60)

                                                                                         CATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAA      <  SRR4302547.2852987/94‑1 (MQ=60)

                                                                                          ATCGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGATTTGCACCAGAGGCTGAACCGCTTAGGGCCAGCAAAACACCCAGCGTCAAT     >  SRR4302547.6505846/1‑94 (MQ=60)

                                                                                          ATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAAT     <  SRR4302547.3655925/94‑1 (MQ=60)

                                                                                            CGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCT   <  SRR4302547.3928289/94‑1 (MQ=60)

                                                                                            CGGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCT   >  SRR4302547.1143714/1‑94 (MQ=60)

                                                                                             GGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  >  SRR4302547.6273479/1‑94 (MQ=60)

                                                                                             GGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  <  SRR4302547.739234/94‑1 (MQ=60)

                                                                                             GGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  <  SRR4302547.6442578/94‑1 (MQ=60)

                                                                                             GGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  <  SRR4302547.2900262/94‑1 (MQ=60)

                                                                                             AGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  <  SRR4302547.2065418/94‑1 (MQ=60)

                                                                                             AGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  <  SRR4302547.1259051/94‑1 (MQ=60)

AGTAATAGTGGTTAAGGAGGCATTACCGCCAGCCAAAATATTAAGCACTCCAGTGCCATACCAGCCAACCAGGTAGGCATCATAAGCAACATCAGTATCAATATTGGTAATCTGATCATTATCAACTTCCAGACTTGCACCAGACGCTGAACCGCTTAGTGCCAGCAAAACACCCAGCGTCAATCTA  >  NC_000913/1595790‑1595976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`