Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,602,535	C→T	F155F (TTC→TTT)	ydeZ →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,602,535	0	C	T	87.1%	535.6 / 52.7	178	F155F (TTC→TTT)	ydeZ	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base C (9/14); new base T (72/83); total (81/97)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

Only 100 of 184 total aligned reads displayed.

GTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATG  >  NC_000913/1602445‑1602626

gTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGc                                                                                           <  2:5129278/93‑1 (MQ=255)

gTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGc                                                                                           <  1:4183371/93‑1 (MQ=255)

 tttGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCt                                                                                          <  2:2912486/93‑1 (MQ=255)

 tttGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCt                                                                                          <  2:3228937/93‑1 (MQ=255)

  ttGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTa                                                                                         >  1:5940446/1‑93 (MQ=255)

   tGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTaa                                                                                        <  2:3759409/93‑1 (MQ=255)

   tGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTaa                                                                                        <  2:2146790/93‑1 (MQ=255)

    gCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAAc                                                                                       >  2:1284126/1‑93 (MQ=255)

    gCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAAc                                                                                       >  2:5020293/1‑93 (MQ=255)

    gCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAAc                                                                                       <  2:3445895/93‑1 (MQ=255)

     ccGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAAcc                                                                                      <  2:5826737/93‑1 (MQ=255)

       ggAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTg                                                                                    >  1:1937413/1‑93 (MQ=255)

       ggAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTg                                                                                    <  1:1639657/93‑1 (MQ=255)

       ggAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGGATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTg                                                                                    >  1:725434/1‑93 (MQ=255)

        gAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTgg                                                                                   <  1:4533714/93‑1 (MQ=255)

        gAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTgg                                                                                   >  1:76444/1‑93 (MQ=255)

          aGCGCTGTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGAt                                                                                 <  1:3454779/93‑1 (MQ=255)

          aGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTggct                                                                                 >  1:4960412/1‑91 (MQ=255)

          aGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGAt                                                                                 <  2:750863/93‑1 (MQ=255)

           gcgcTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGAtg                                                                                >  2:3847405/1‑93 (MQ=255)

            cgcTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGAtgt                                                                               >  2:2572956/1‑93 (MQ=255)

             gcTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGAtgtg                                                                              <  1:266522/93‑1 (MQ=255)

               tctGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCt                                                                            >  1:2878134/1‑93 (MQ=255)

                ctgctgCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTg                                                                           >  2:4934300/1‑93 (MQ=255)

                ctgctgCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTg                                                                           <  2:1374587/93‑1 (MQ=255)

                ctgctgCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTg                                                                           >  1:2099695/1‑93 (MQ=255)

                 tgctgcTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTgg                                                                          >  1:1610829/1‑93 (MQ=255)

                  gctgctTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTggg                                                                         <  1:5946206/93‑1 (MQ=255)

                   ctgctTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGa                                                                        >  2:3670635/1‑93 (MQ=255)

                   ctgctTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGa                                                                        <  1:4701211/93‑1 (MQ=255)

                    ttctTGCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGAc                                                                       <  1:6426106/91‑1 (MQ=255)

                     gctTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACt                                                                      <  2:4025441/93‑1 (MQ=255)

                      ctTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTc                                                                     >  1:3303758/1‑93 (MQ=255)

                            ggTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGtt                                                               >  1:3679186/1‑93 (MQ=255)

                              tATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTcc                                                             >  2:3833636/1‑93 (MQ=255)

                               aTGGCCGGAGCGACGGGGTACGAAGGCATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTGACCTGGATGTGCTGGGACTCCCCGTTCCg                                                            >  2:6082047/1‑93 (MQ=255)

                                tGGCCGGAGCGACTGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGc                                                           <  1:1646914/93‑1 (MQ=255)

                                tGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGc                                                           <  1:836476/93‑1 (MQ=255)

                                 ggCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCt                                                          <  1:5781205/93‑1 (MQ=255)

                                 ggCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCt                                                          <  1:4284179/93‑1 (MQ=255)

                                 ggCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCt                                                          <  1:388576/93‑1 (MQ=255)

                                  gCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTg                                                         >  2:1388647/1‑93 (MQ=255)

                                    cGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGAt                                                       <  1:1196172/93‑1 (MQ=255)

                                    cGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGAt                                                       >  2:3068563/1‑93 (MQ=255)

                                     ggAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGAtt                                                      >  2:2442933/1‑93 (MQ=255)

                                      gAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTa                                                     >  2:3344752/1‑93 (MQ=255)

                                        gCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTAtt                                                   >  1:2624849/1‑92 (MQ=255)

                                         cGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCt                                                  <  1:3758290/93‑1 (MQ=255)

                                          gACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAGCCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCtt                                                 <  2:1323161/93‑1 (MQ=255)

                                            cGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTcc                                               <  1:6120661/93‑1 (MQ=255)

                                            cGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTcc                                               >  1:1467469/1‑93 (MQ=255)

                                             ggggTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCt                                              >  1:3261374/1‑93 (MQ=255)

                                               ggTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGa                                            <  2:1206276/93‑1 (MQ=255)

                                                gTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGat                                           >  1:4275658/1‑93 (MQ=255)

                                                gTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGat                                           <  2:4807352/93‑1 (MQ=255)

                                                 tACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGata                                          <  1:2010726/93‑1 (MQ=255)

                                                  aCGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGatat                                         >  2:2024375/1‑93 (MQ=255)

                                                  aCGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGatat                                         <  1:4092021/93‑1 (MQ=255)

                                                    gAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGt                                       >  1:6289608/1‑93 (MQ=255)

                                                     aaGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGtc                                      <  1:3436165/93‑1 (MQ=255)

                                                      aGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGtct                                     >  2:5154303/1‑93 (MQ=255)

                                                       ggTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGtctc                                    <  1:4327489/93‑1 (MQ=255)

                                                       ggTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGtctc                                    <  2:5694393/93‑1 (MQ=255)

                                                        gTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTctcc                                   <  2:859856/93‑1 (MQ=255)

                                                         tATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTctcct                                  <  1:178581/93‑1 (MQ=255)

                                                         tATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTctcct                                  <  1:4848118/93‑1 (MQ=255)

                                                            tggtggATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGt                               >  1:1077145/1‑93 (MQ=255)

                                                            tggtggATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGt                               >  1:4520823/1‑93 (MQ=255)

                                                                ggATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtc                           <  1:933805/93‑1 (MQ=255)

                                                                 gATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtct                          >  2:4262208/1‑93 (MQ=255)

                                                                 gATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtct                          <  2:1174634/93‑1 (MQ=255)

                                                                 gATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtct                          <  2:6148104/93‑1 (MQ=255)

                                                                  aTTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctg                         <  1:620208/93‑1 (MQ=255)

                                                                   ttCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctgg                        >  2:4355685/1‑93 (MQ=255)

                                                                   ttCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctgg                        >  1:6166301/1‑93 (MQ=255)

                                                                   ttCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctgg                        >  2:5199142/1‑93 (MQ=255)

                                                                   ttCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctgg                        >  1:4909381/1‑93 (MQ=255)

                                                                    tCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggc                       <  1:1322514/93‑1 (MQ=255)

                                                                    tCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggc                       >  2:495204/1‑93 (MQ=255)

                                                                      ccGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggctc                     <  1:364884/93‑1 (MQ=255)

                                                                       cGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggctct                    <  1:215888/93‑1 (MQ=255)

                                                                       cGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggctct                    >  1:2904540/1‑93 (MQ=255)

                                                                          tGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggctctggc                 <  2:626097/93‑1 (MQ=255)

                                                                          tGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTtctggctctggc                 >  1:103256/1‑93 (MQ=255)

                                                                              gTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCa             <  2:6341026/93‑1 (MQ=255)

                                                                              gTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCa             >  2:1737940/1‑93 (MQ=255)

                                                                              gTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCa             >  1:2955562/1‑93 (MQ=255)

                                                                               tttACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCAt            >  2:4805618/1‑93 (MQ=255)

                                                                               tttACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCAt            >  2:1995842/1‑93 (MQ=255)

                                                                               tttACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGCTTTCTGGCTCTGGCTGCAt            >  1:2294321/1‑93 (MQ=255)

                                                                                 tACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATaa          <  1:2064747/93‑1 (MQ=255)

                                                                                 tACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATaa          <  2:5999970/93‑1 (MQ=255)

                                                                                  aCAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATaaa         <  2:557556/93‑1 (MQ=255)

                                                                                   cAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATaaaa        >  1:5311831/1‑93 (MQ=255)

                                                                                    aGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAAc       >  2:4212743/1‑93 (MQ=255)

                                                                                    aGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAAc       <  2:1112881/93‑1 (MQ=255)

                                                                                     gATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAAcc      >  1:1410573/1‑93 (MQ=255)

                                                                                     gATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAAcc      >  1:5397631/1‑93 (MQ=255)

                                                                                       ttttGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCa    <  2:6539732/93‑1 (MQ=255)

                                                                                         tCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATg  <  2:2979379/93‑1 (MQ=255)

GTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATG  >  NC_000913/1602445‑1602626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTGTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCC  >  NC_000913/1602442‑1602628

TCTGTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTT                                                                                               <  SRR4302547.4818797/94‑1 (MQ=60)

TCTGTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTT                                                                                               <  SRR4302547.3948711/94‑1 (MQ=60)

 CTGTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGATTACAGATTTTG                                                                                              >  SRR4302547.2240331/1‑94 (MQ=60)

   GTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCT                                                                                            <  SRR4302547.4268630/94‑1 (MQ=60)

    TTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGTGTACGAAGGTATTGGTGGATTCTCGATGGCGTTTACAGATTTTGCTA                                                                                           <  SRR4302547.5451154/94‑1 (MQ=60)

    TTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTA                                                                                           >  SRR4302547.6063617/1‑94 (MQ=60)

         CGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTG                                                                                      >  SRR4302547.1975839/1‑94 (MQ=60)

         CGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGGATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTG                                                                                      >  SRR4302547.738949/1‑94 (MQ=60)

          GGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGG                                                                                     >  SRR4302547.77880/1‑94 (MQ=60)

          GGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGG                                                                                     <  SRR4302547.3839509/94‑1 (MQ=60)

          GGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGG                                                                                     <  SRR4302547.1672069/94‑1 (MQ=60)

           GAAGCTCTCTGCTGCTTGCCGGTATGGCCGGAGCGACGGGGTACGAAGGTAGTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGA                                                                                    <  SRR4302547.1320012/94‑1 (MQ=60)

           GAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGA                                                                                    <  SRR4302547.4627411/94‑1 (MQ=60)

           GAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGA                                                                                    >  SRR4302547.6272648/1‑94 (MQ=60)

            AAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGCT                                                                                   >  SRR4302547.5063056/1‑94 (MQ=60)

            AAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGAT                                                                                   <  SRR4302547.4318859/94‑1 (MQ=60)

             AGCGCTGTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATG                                                                                  <  SRR4302547.3525209/94‑1 (MQ=60)

              GCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGT                                                                                 <  SRR4302547.4232434/94‑1 (MQ=60)

                GCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGC                                                                               <  SRR4302547.271511/94‑1 (MQ=60)

                 CTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCT                                                                              >  SRR4302547.2935589/1‑94 (MQ=60)

                  TCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTG                                                                             >  SRR4302547.2141548/1‑94 (MQ=60)

                  TCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTG                                                                             >  SRR4302547.1007798/1‑94 (MQ=60)

                   CTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGG                                                                            >  SRR4302547.1642740/1‑94 (MQ=60)

                     GCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGA                                                                          <  SRR4302547.6069508/94‑1 (MQ=60)

                     GCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGA                                                                          <  SRR4302547.1946807/94‑1 (MQ=60)

                     GCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGA                                                                          <  SRR4302547.5535021/94‑1 (MQ=60)

                      CTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGAC                                                                         <  SRR4302547.4798577/94‑1 (MQ=60)

                       TTCTTGCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACT                                                                        <  SRR4302547.6560081/94‑1 (MQ=60)

                       TGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGAATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACT                                                                        >  SRR4302547.813969/1‑94 (MQ=60)

                        GCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTC                                                                       <  SRR4302547.1577068/94‑1 (MQ=60)

                        GCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTC                                                                       >  SRR4302547.3370293/1‑94 (MQ=60)

                         CTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGCTGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTAC                                                                      >  SRR4302547.2258807/1‑94 (MQ=60)

                           TTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCC                                                                    <  SRR4302547.4090096/94‑1 (MQ=60)

                            TCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCG                                                                   <  SRR4302547.3796959/94‑1 (MQ=60)

                             CCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGT                                                                  <  SRR4302547.3316659/94‑1 (MQ=60)

                              CGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTT                                                                 >  SRR4302547.3754299/1‑94 (MQ=60)

                               GGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACGCCCCGTTC                                                                <  SRR4302547.4701211/94‑1 (MQ=60)

                                 TATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTACCCTGGATGTGCTGGGACTCCCCGTTCCG                                                              <  SRR4302547.3521696/94‑1 (MQ=60)

                                   TGGCCGGAGCGACTGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCT                                                            <  SRR4302547.1679442/94‑1 (MQ=60)

                                   TGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCT                                                            <  SRR4302547.852447/94‑1 (MQ=60)

                                    GGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTG                                                           <  SRR4302547.4372203/94‑1 (MQ=60)

                                    GGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTG                                                           <  SRR4302547.395782/94‑1 (MQ=60)

                                    GGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTG                                                           <  SRR4302547.5900778/94‑1 (MQ=60)

                                    GGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTG                                                           <  SRR4302547.2422469/94‑1 (MQ=60)

                                    GGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTG                                                           >  SRR4302547.6322359/1‑94 (MQ=60)

                                      CCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGAT                                                         <  SRR4302547.2085640/94‑1 (MQ=60)

                                       CGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATT                                                        <  SRR4302547.1220309/94‑1 (MQ=60)

                                       CGGAGCGACGGGGGACGAAGGGATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGGGCTGGGACTCCCCGTTCCGCTGATT                                                        >  SRR4302547.3648547/1‑94 (MQ=60)

                                        GGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTA                                                       <  SRR4302547.1457896/94‑1 (MQ=60)

                                          AGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATT                                                     >  SRR4302547.2677724/1‑94 (MQ=60)

                                          AGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATC                                                     >  SRR4302547.5103512/1‑94 (MQ=60)

                                            CGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTT                                                   <  SRR4302547.3834976/94‑1 (MQ=60)

                                              ACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCC                                                 <  SRR4302547.267823/94‑1 (MQ=60)

                                              ACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCC                                                 >  SRR4302547.1496789/1‑94 (MQ=60)

                                               CGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCT                                                <  SRR4302547.6247634/94‑1 (MQ=60)

                                               CGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCT                                                >  SRR4302547.3326588/1‑94 (MQ=60)

                                               CGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCT                                                <  SRR4302547.813675/94‑1 (MQ=60)

                                                GGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTG                                               >  SRR4302547.1146367/1‑94 (MQ=60)

                                                 GGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGA                                              <  SRR4302547.6453855/94‑1 (MQ=60)

                                                  GGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGAT                                             >  SRR4302547.4363468/1‑94 (MQ=60)

                                                   GTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATA                                            >  SRR4302547.4480926/1‑94 (MQ=60)

                                                    TACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATAT                                           <  SRR4302547.2050734/94‑1 (MQ=60)

                                                     ACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATG                                          <  SRR4302547.4175231/94‑1 (MQ=60)

                                                      CGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGT                                         >  SRR4302547.6151981/1‑94 (MQ=60)

                                                      CGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGT                                         >  SRR4302547.6420308/1‑94 (MQ=60)

                                                       GAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTC                                        >  SRR4302547.4615034/1‑94 (MQ=60)

                                                        AAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCT                                       <  SRR4302547.3506222/94‑1 (MQ=60)

                                                          GGTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCC                                     <  SRR4302547.4416555/94‑1 (MQ=60)

                                                           GTATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCT                                    >  SRR4302547.2167531/1‑94 (MQ=60)

                                                            TATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTC                                   <  SRR4302547.6693008/94‑1 (MQ=60)

                                                            TATTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTC                                   <  SRR4302547.181928/94‑1 (MQ=60)

                                                            TATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTC                                   <  SRR4302547.4948592/94‑1 (MQ=60)

                                                              TTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGT                                 >  SRR4302547.4614231/1‑94 (MQ=60)

                                                              TTGGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGT                                 >  SRR4302547.1098638/1‑94 (MQ=60)

                                                                GGTGGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTT                               <  SRR4302547.5910057/94‑1 (MQ=60)

                                                                   GGATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCT                            <  SRR4302547.951868/94‑1 (MQ=60)

                                                                     ATTCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGG                          >  SRR4302547.6294287/1‑94 (MQ=60)

                                                                     ATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGG                          >  SRR4302547.5011052/1‑94 (MQ=60)

                                                                     ATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGG                          <  SRR4302547.631683/94‑1 (MQ=60)

                                                                       TCCCGATGGCGTTTACAGATTTTGCTAGCCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCT                        <  SRR4302547.1793082/94‑1 (MQ=60)

                                                                       TCCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCT                        <  SRR4302547.1349095/94‑1 (MQ=60)

                                                                        CCCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTTGCTC                       <  SRR4302547.2015205/94‑1 (MQ=60)

                                                                         CCGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCT                      <  SRR4302547.371659/94‑1 (MQ=60)

                                                                         CCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCT                      >  SRR4302547.2962459/1‑94 (MQ=60)

                                                                          CGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTG                     <  SRR4302547.219954/94‑1 (MQ=60)

                                                                          CGATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTG                     >  SRR4302547.6371077/1‑94 (MQ=60)

                                                                          CGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTG                     <  SRR4302547.1376243/94‑1 (MQ=60)

                                                                           GATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGG                    <  SRR4302547.3339569/94‑1 (MQ=60)

                                                                            ATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGC                   <  SRR4302547.2766153/94‑1 (MQ=60)

                                                                            ATGGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGC                   >  SRR4302547.105145/1‑94 (MQ=60)

                                                                              GGCGTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTG                 <  SRR4302547.91922/94‑1 (MQ=60)

                                                                                CGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCA               >  SRR4302547.3014389/1‑94 (MQ=60)

                                                                                 GTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCAT              <  SRR4302547.5026992/94‑1 (MQ=60)

                                                                                 GTTTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGCTTTCTGGCTCTGGCTGCAT              >  SRR4302547.2340788/1‑94 (MQ=60)

                                                                                   TTACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAA            >  SRR4302547.4822496/1‑94 (MQ=60)

                                                                                    TACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAA           <  SRR4302547.2105946/94‑1 (MQ=60)

                                                                                    TACAGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAA           >  SRR4302547.1237355/1‑94 (MQ=60)

                                                                                     ACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAA          >  SRR4302547.5421388/1‑94 (MQ=60)

                                                                                       AGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACC        >  SRR4302547.5509164/1‑94 (MQ=60)

                                                                                       AGATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACC        >  SRR4302547.1438788/1‑94 (MQ=60)

                                                                                        GATTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCC       <  SRR4302547.1679407/94‑1 (MQ=60)

                                                                                          TTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCAT     <  SRR4302547.343784/94‑1 (MQ=60)

                                                                                          TTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCAT     <  SRR4302547.4390380/94‑1 (MQ=60)

                                                                                          TTTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCAT     <  SRR4302547.3011458/94‑1 (MQ=60)

                                                                                           TTTGCTAACCTGTATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATG    <  SRR4302547.4234636/94‑1 (MQ=60)

                                                                                           TTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATG    <  SRR4302547.3301161/94‑1 (MQ=60)

                                                                                           TTTGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATG    <  SRR4302547.3071871/94‑1 (MQ=60)

                                                                                             TGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCC  <  SRR4302547.231062/94‑1 (MQ=60)

                                                                                             TGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCC  <  SRR4302547.5454022/94‑1 (MQ=60)

                                                                                             TGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCC  >  SRR4302547.3294205/1‑94 (MQ=60)

TCTGTTTGCCGGAAGCGCTCTGCTGCTTTCCGGTATGGCCGGAGCGACGGGGTACGAAGGTATTGGTGGATTCCCGATGGCGTTTACAGATTTCGCTAACCTGGATGTGCTGGGACTCCCCGTTCCGCTGATTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCC  >  NC_000913/1602442‑1602628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`