Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,609,260 G→A H207Y (CAT→TAT)  b1522 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,2600GA86.8% 691.1 / 76.6 250H207Y (CAT→TAT) b1522hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/17);  new base A (97/119);  total (113/137)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-02

Only 100 of 256 total aligned reads displayed.

CGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGA  >  NC_000913/1609170‑1609349
                                                                                          |                                                                                         
cGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATAcc                                                                                         <  2:6229256/93‑1 (MQ=255)
 gACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCg                                                                                        <  2:429147/93‑1 (MQ=255)
  aCAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCgg                                                                                       >  2:1795811/1‑93 (MQ=255)
  aCAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCgg                                                                                       >  2:1388379/1‑93 (MQ=255)
  aCAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCgg                                                                                       <  1:3734435/93‑1 (MQ=255)
   cAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCTCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCggg                                                                                      <  2:1189811/93‑1 (MQ=255)
     ggATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCa                                                                                    >  1:6147361/1‑93 (MQ=255)
          cACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAacac                                                                               >  1:5890257/1‑93 (MQ=255)
          cACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAacac                                                                               >  2:2693207/1‑93 (MQ=255)
          cACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAacac                                                                               <  2:1425510/93‑1 (MQ=255)
           aCCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACg                                                                              >  2:5713802/1‑93 (MQ=255)
            ccGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGc                                                                             <  1:3340798/93‑1 (MQ=255)
             cGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGTGCAAACACGCt                                                                            <  1:4222464/93‑1 (MQ=255)
             cGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCt                                                                            <  1:1037033/93‑1 (MQ=255)
               aCGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTa                                                                          >  1:5811757/1‑93 (MQ=255)
                 ggCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCaaa                                                                                  <  2:238704/83‑1 (MQ=255)
                  gCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAaca                                                                       >  2:2575946/1‑93 (MQ=255)
                  gCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAaca                                                                       >  1:5030745/1‑93 (MQ=255)
                   cACTGCAACAGCAAATTCTTCGCCGCGCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAacac                                                                      >  1:4237168/1‑93 (MQ=255)
                   cACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAacac                                                                      <  1:6208162/93‑1 (MQ=255)
                     cTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACtt                                                                    >  1:2358180/1‑93 (MQ=255)
                      tGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACttt                                                                   <  2:3624752/93‑1 (MQ=255)
                         aaCAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATc                                                                <  1:2044015/93‑1 (MQ=255)
                             gCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCca                                                            <  2:2264561/93‑1 (MQ=255)
                             gCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTAAATATACCGGGCAAACACGCTTAACACTTTATCGCca                                                            <  2:1491309/93‑1 (MQ=255)
                               aaaTTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCcaca                                                          <  1:4819297/93‑1 (MQ=255)
                                aaTTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACAt                                                         <  2:6561071/93‑1 (MQ=255)
                                 aTTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACAtt                                                        <  1:5388747/93‑1 (MQ=255)
                                  ttcttcGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTc                                                       >  1:3965944/1‑93 (MQ=255)
                                   tcttcGCCGCCCATTCGCGCCCCCAGCCCCTTATCACCGTCAATCTTCTGACTCTACCGGGCCAACCCGCTTCACACTTTATCGCCACATTCa                                                      <  2:6648472/93‑1 (MQ=255)
                                     ttcGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATg                                                    >  1:5828263/1‑93 (MQ=255)
                                     ttcGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATg                                                    <  1:2968855/93‑1 (MQ=255)
                                        gccgccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTcc                                                 >  2:1507251/1‑93 (MQ=255)
                                        gccgccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTcc                                                 <  1:1580403/93‑1 (MQ=255)
                                        gccgccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTcc                                                 >  1:721492/1‑93 (MQ=255)
                                        cccgccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTcc                                                 <  2:1652187/92‑1 (MQ=255)
                                          cgccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCAt                                               <  2:836/93‑1 (MQ=255)
                                          cgccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCAt                                               <  2:5933080/93‑1 (MQ=255)
                                           gccCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATa                                              <  1:438118/93‑1 (MQ=255)
                                            cccATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAg                                             <  2:4115215/93‑1 (MQ=255)
                                            cccATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAg                                             >  2:2326375/1‑93 (MQ=255)
                                            cccATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGACACATTCATGTCCATAg                                             >  2:1293377/1‑93 (MQ=255)
                                               aTTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTa                                          >  2:910372/1‑93 (MQ=255)
                                               aTTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTa                                          >  1:3638171/1‑93 (MQ=255)
                                                ttCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAt                                         >  1:6351530/1‑93 (MQ=255)
                                                ttCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAt                                         >  1:1154444/1‑93 (MQ=255)
                                                ttCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAt                                         >  2:4897806/1‑93 (MQ=255)
                                                ttCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAt                                         >  2:2821306/1‑93 (MQ=255)
                                                  cgcgCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATct                                       >  1:5615159/1‑92 (MQ=255)
                                                   gcgcCACCAGCCCCTTATCTCCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGt                                      >  1:6465796/1‑93 (MQ=255)
                                                   gcgcCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGt                                      <  2:2638046/93‑1 (MQ=255)
                                                   gcgcCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGt                                      <  1:3035455/93‑1 (MQ=255)
                                                     gcCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTa                                    >  1:5086917/1‑93 (MQ=255)
                                                       caccaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                                  <  2:3081564/93‑1 (MQ=255)
                                                       caccaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAAt                                  <  2:649175/93‑1 (MQ=255)
                                                        cccaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATg                                 <  2:1620168/92‑1 (MQ=255)
                                                        cccaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATg                                 <  2:1111695/92‑1 (MQ=255)
                                                        accaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATg                                 <  1:5239996/93‑1 (MQ=255)
                                                         ccaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGc                                >  1:4448530/1‑93 (MQ=255)
                                                         ccaGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGc                                <  1:328211/93‑1 (MQ=255)
                                                           aGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCtt                              >  1:283454/1‑93 (MQ=255)
                                                           aGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCtt                              >  2:5577865/1‑93 (MQ=255)
                                                             ccccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCtttt                            <  1:1738367/93‑1 (MQ=255)
                                                             ccccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCtttt                            >  1:2555759/1‑93 (MQ=255)
                                                             ccccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCtttt                            <  2:5198280/93‑1 (MQ=255)
                                                              cccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTg                           <  2:2989698/93‑1 (MQ=255)
                                                              cccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTg                           >  2:3679164/1‑93 (MQ=255)
                                                               ccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGa                          >  1:5454350/1‑93 (MQ=255)
                                                               ccTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGa                          <  2:5821989/93‑1 (MQ=255)
                                                                 ttATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGaaa                        <  1:2124731/93‑1 (MQ=255)
                                                                     cACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGt                    <  1:5680247/93‑1 (MQ=255)
                                                                       ccGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCg                  >  2:4437559/1‑93 (MQ=255)
                                                                        cGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGa                 >  1:2640171/1‑93 (MQ=255)
                                                                        cGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGa                 <  2:4061868/93‑1 (MQ=255)
                                                                         gACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGat                <  2:318341/93‑1 (MQ=255)
                                                                         gACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGat                >  2:3506909/1‑93 (MQ=255)
                                                                         gACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGat                >  2:1978362/1‑93 (MQ=255)
                                                                         gACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGat                >  2:6279718/1‑93 (MQ=255)
                                                                         gACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGat                <  1:2390102/93‑1 (MQ=255)
                                                                         gACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGat                >  2:4589373/1‑93 (MQ=255)
                                                                           cAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGatat              <  2:3019516/93‑1 (MQ=255)
                                                                            aaTCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATc             >  2:4873498/1‑93 (MQ=255)
                                                                            aaTCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATc             >  2:380283/1‑93 (MQ=255)
                                                                             aTCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCa            >  1:135703/1‑93 (MQ=255)
                                                                              tcttctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCaa           >  1:1106690/1‑93 (MQ=255)
                                                                               cttctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAag          <  1:4913900/93‑1 (MQ=255)
                                                                               cttctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAag          >  1:715869/1‑93 (MQ=255)
                                                                                ttctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagc         <  2:2923609/93‑1 (MQ=255)
                                                                                ttctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagc         >  1:1296791/1‑93 (MQ=255)
                                                                                 tctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagca        >  1:2165626/1‑93 (MQ=255)
                                                                                 tctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGAGATCAagca        <  1:6491571/93‑1 (MQ=255)
                                                                                  ctGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagcag       <  1:5969218/93‑1 (MQ=255)
                                                                                  ctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagcag       <  2:1845813/93‑1 (MQ=255)
                                                                                  ctGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagcag       >  2:5025610/1‑93 (MQ=255)
                                                                                   tGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAagcagc      >  1:4114184/1‑93 (MQ=255)
                                                                                    taaTATACCGGGCAAACACGCTTAACGCTTTATCGCCACATTCATGTCAATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAgcagca     <  1:3597252/92‑1 (MQ=255)
                                                                                     aaTATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCAt    <  2:332854/93‑1 (MQ=255)
                                                                                       tataCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGa  >  2:5269463/1‑93 (MQ=255)
                                                                                       tatGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGa  <  2:6112347/93‑1 (MQ=255)
                                                                                       tatGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGa  <  1:1329577/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGA  >  NC_000913/1609170‑1609349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGACA  >  NC_000913/1609167‑1609351
                                                                                             |                                                                                           
CATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATA                                                                                             >  SRR4302547.6573335/1‑94 (MQ=60)
CATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATA                                                                                             >  SRR4302547.5465647/1‑94 (MQ=60)
CATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATA                                                                                             <  SRR4302547.2190970/94‑1 (MQ=60)
 ATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATAC                                                                                            >  SRR4302547.4408160/1‑94 (MQ=60)
  ccgcCAGGATTCCCCGCCGGCACTGCAACAGCAATTTCTCCGCCCCCCATCCGCGCCCCCACCCCTTTACCACCGACAATCTTCTGAATATCCC                                                                                           <  SRR4302547.2201181/90‑1 (MQ=29)
  TCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACC                                                                                           <  SRR4302547.1764207/94‑1 (MQ=60)
     ACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGG                                                                                        >  SRR4302547.5064359/1‑94 (MQ=60)
     ACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGG                                                                                        <  SRR4302547.1399572/94‑1 (MQ=60)
     ACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGG                                                                                        <  SRR4302547.3810685/94‑1 (MQ=60)
      CAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGC                                                                                       <  SRR4302547.5902984/94‑1 (MQ=60)
       AGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCA                                                                                      >  SRR4302547.6274898/1‑94 (MQ=60)
       AGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCA                                                                                      >  SRR4302547.5992572/1‑94 (MQ=60)
       AGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCA                                                                                      <  SRR4302547.5140758/94‑1 (MQ=60)
       AGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCA                                                                                      >  SRR4302547.2351702/1‑94 (MQ=60)
          ATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAAC                                                                                   >  SRR4302547.4077537/1‑94 (MQ=60)
            TCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACAC                                                                                 >  SRR4302547.6012203/1‑94 (MQ=60)
              ACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGC                                                                               >  SRR4302547.6388127/1‑94 (MQ=60)
              ACAGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGC                                                                               <  SRR4302547.1220394/94‑1 (MQ=60)
               CCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCT                                                                              <  SRR4302547.5945999/94‑1 (MQ=60)
               CCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCT                                                                              <  SRR4302547.3408412/94‑1 (MQ=60)
                CGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGTGCAAACACGCTT                                                                             <  SRR4302547.4308706/94‑1 (MQ=60)
                CGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTT                                                                             <  SRR4302547.2609666/94‑1 (MQ=60)
                CGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTT                                                                             <  SRR4302547.5998337/94‑1 (MQ=60)
                CGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTT                                                                             <  SRR4302547.1057460/94‑1 (MQ=60)
                 GACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTA                                                                            >  SRR4302547.4317633/1‑94 (MQ=60)
                 GACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTA                                                                            >  SRR4302547.5931999/1‑94 (MQ=60)
                 GACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTA                                                                            >  SRR4302547.6526682/1‑94 (MQ=60)
                 GACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTA                                                                            >  SRR4302547.1072134/1‑94 (MQ=60)
                 GACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTA                                                                            <  SRR4302547.3395325/94‑1 (MQ=60)
                   CGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACAatgtcaag                                                                          >  SRR4302547.243175/1‑86 (MQ=60)
                    GGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACA                                                                         >  SRR4302547.5134685/1‑94 (MQ=60)
                     GCACTGCAACAGCAAATTCTTCGCCGCGCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACAC                                                                        >  SRR4302547.4323803/1‑94 (MQ=60)
                     GCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACAC                                                                        >  SRR4302547.1368995/1‑94 (MQ=60)
                      CACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACT                                                                       <  SRR4302547.6337068/94‑1 (MQ=60)
                       ACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTT                                                                      >  SRR4302547.966795/1‑94 (MQ=60)
                       ACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTT                                                                      >  SRR4302547.2405967/1‑94 (MQ=60)
                          TCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTAT                                                                   <  SRR4302547.521596/94‑1 (MQ=60)
                            AACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCG                                                                 >  SRR4302547.4074743/1‑94 (MQ=60)
                            AACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCG                                                                 <  SRR4302547.6290073/94‑1 (MQ=60)
                            AACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCG                                                                 <  SRR4302547.220228/94‑1 (MQ=60)
                            AACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCG                                                                 <  SRR4302547.2084791/94‑1 (MQ=60)
                               AGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCA                                                              <  SRR4302547.3695535/94‑1 (MQ=60)
                                 CAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACA                                                            <  SRR4302547.3949421/94‑1 (MQ=60)
                                  AAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACAT                                                           <  SRR4302547.4919168/94‑1 (MQ=60)
                                   AATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATT                                                          <  SRR4302547.5445363/94‑1 (MQ=60)
                                    ATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTC                                                         >  SRR4302547.4046599/1‑94 (MQ=60)
                                    ATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTC                                                         <  SRR4302547.5500025/94‑1 (MQ=60)
                                    ATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTC                                                         <  SRR4302547.3472739/94‑1 (MQ=60)
                                     TTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATCCCGGGCACACACGCTTAACACTTTATCGCCACATTCA                                                        <  SRR4302547.5754391/94‑1 (MQ=60)
                                     TTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCA                                                        >  SRR4302547.4456325/1‑94 (MQ=60)
                                      TCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCAT                                                       <  SRR4302547.3047861/94‑1 (MQ=60)
                                      TCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCAT                                                       <  SRR4302547.6670730/94‑1 (MQ=60)
                                       CTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATG                                                      <  SRR4302547.2326299/94‑1 (MQ=60)
                                       CTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATG                                                      <  SRR4302547.2853210/94‑1 (MQ=60)
                                       CTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATG                                                      >  SRR4302547.5948877/1‑94 (MQ=60)
                                        TTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGT                                                     <  SRR4302547.3027948/94‑1 (MQ=60)
                                          TGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTGCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCC                                                   <  SRR4302547.3766427/94‑1 (MQ=60)
                                          CGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCC                                                   >  SRR4302547.734923/1‑94 (MQ=60)
                                           GCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCA                                                  <  SRR4302547.1611857/94‑1 (MQ=60)
                                             CGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATA                                                <  SRR4302547.4440332/94‑1 (MQ=60)
                                              GCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAG                                               <  SRR4302547.446282/94‑1 (MQ=60)
                                              GCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAG                                               >  SRR4302547.4624871/1‑94 (MQ=60)
                                              CCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAG                                               <  SRR4302547.1349553/94‑1 (MQ=60)
                                               CCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGT                                              >  SRR4302547.6089409/1‑94 (MQ=60)
                                                CCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTT                                             >  SRR4302547.3049053/1‑94 (MQ=60)
                                                CCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTT                                             >  SRR4302547.1161709/1‑94 (MQ=60)
                                                CCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTT                                             <  SRR4302547.2838792/94‑1 (MQ=60)
                                                 CATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTA                                            >  SRR4302547.3712369/1‑94 (MQ=60)
                                                  ATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAT                                           <  SRR4302547.4929317/94‑1 (MQ=60)
                                                  ATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAT                                           >  SRR4302547.6483885/1‑94 (MQ=60)
                                                  ATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTAT                                           >  SRR4302547.1177801/1‑94 (MQ=60)
                                                   TTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATC                                          <  SRR4302547.6751710/94‑1 (MQ=60)
                                                    TCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCT                                         >  SRR4302547.5731035/1‑94 (MQ=60)
                                                     CGCGCCACCAGCCCCTTATCTCCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGT                                        >  SRR4302547.6600436/1‑94 (MQ=60)
                                                      GCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTT                                       <  SRR4302547.1663070/94‑1 (MQ=60)
                                                      GCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTT                                       <  SRR4302547.3095839/94‑1 (MQ=60)
                                                       CGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTA                                      >  SRR4302547.5191843/1‑94 (MQ=60)
                                                          CACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATG                                   <  SRR4302547.6590128/94‑1 (MQ=60)
                                                          CACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATG                                   <  SRR4302547.4207296/94‑1 (MQ=60)
                                                           ACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGC                                  >  SRR4302547.4540513/1‑94 (MQ=60)
                                                           ACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGC                                  >  SRR4302547.1157406/1‑94 (MQ=60)
                                                           ACCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGC                                  <  SRR4302547.5347858/94‑1 (MQ=60)
                                                            CCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCT                                 >  SRR4302547.2606206/1‑94 (MQ=60)
                                                            CCAGCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCT                                 <  SRR4302547.334317/94‑1 (MQ=60)
                                                             CAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTT                                >  SRR4302547.288723/1‑94 (MQ=60)
                                                               GCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTT                              >  SRR4302547.2607368/1‑94 (MQ=60)
                                                               GCCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTT                              <  SRR4302547.5567490/94‑1 (MQ=60)
                                                                CCCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTG                             <  SRR4302547.1772543/94‑1 (MQ=60)
                                                                 CCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGA                            <  SRR4302547.6293156/94‑1 (MQ=60)
                                                                 CCCTTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGA                            >  SRR4302547.5567053/1‑94 (MQ=60)
                                                                    TTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAAT                         >  SRR4302547.1346014/1‑94 (MQ=60)
                                                                    TTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAAT                         <  SRR4302547.6098541/94‑1 (MQ=60)
                                                                    TTATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAAT                         <  SRR4302547.2167072/94‑1 (MQ=60)
                                                                     TATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATA                        <  SRR4302547.213772/94‑1 (MQ=60)
                                                                     TATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATA                        <  SRR4302547.5643711/94‑1 (MQ=60)
                                                                      ATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAG                       >  SRR4302547.3338623/1‑94 (MQ=60)
                                                                      ATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAG                       >  SRR4302547.2464439/1‑94 (MQ=60)
                                                                      ATCACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAG                       <  SRR4302547.732092/94‑1 (MQ=60)
                                                                       TCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGT                      <  SRR4302547.5239562/94‑1 (MQ=60)
                                                                        CACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTC                     <  SRR4302547.5797533/94‑1 (MQ=60)
                                                                         ACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCG                    >  SRR4302547.6250469/1‑94 (MQ=60)
                                                                         ACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCG                    >  SRR4302547.4498974/1‑94 (MQ=60)
                                                                         ACCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCG                    >  SRR4302547.5354604/1‑94 (MQ=60)
                                                                          CCGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGA                   >  SRR4302547.2693317/1‑94 (MQ=60)
                                                                           CGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGAT                  >  SRR4302547.2985927/1‑94 (MQ=60)
                                                                           CGACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGAT                  >  SRR4302547.4123319/1‑94 (MQ=60)
                                                                            GACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATA                 >  SRR4302547.1559439/1‑94 (MQ=60)
                                                                            GACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATA                 <  SRR4302547.2438516/94‑1 (MQ=60)
                                                                            GACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATA                 <  SRR4302547.4816363/94‑1 (MQ=60)
                                                                             ACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATAT                <  SRR4302547.348484/94‑1 (MQ=60)
                                                                             ACAATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATAT                <  SRR4302547.1945978/94‑1 (MQ=60)
                                                                               AATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATCTCA              >  SRR4302547.3730830/1‑94 (MQ=60)
                                                                               AATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCA              <  SRR4302547.5528401/94‑1 (MQ=60)
                                                                               AATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCA              >  SRR4302547.138152/1‑94 (MQ=60)
                                                                                ATCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAA             >  SRR4302547.1128968/1‑94 (MQ=60)
                                                                                 TCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAG            >  SRR4302547.729129/1‑94 (MQ=60)
                                                                                 TCTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAG            <  SRR4302547.2047148/94‑1 (MQ=60)
                                                                                  CTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGC           >  SRR4302547.3306338/1‑94 (MQ=60)
                                                                                  CTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGC           >  SRR4302547.1322897/1‑94 (MQ=60)
                                                                                  CTTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGC           <  SRR4302547.5015661/94‑1 (MQ=60)
                                                                                   TTCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCA          >  SRR4302547.2208937/1‑94 (MQ=60)
                                                                                    TCTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGAGATCAAGCAG         <  SRR4302547.6626724/94‑1 (MQ=60)
                                                                                     CTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGC        <  SRR4302547.6093026/94‑1 (MQ=60)
                                                                                     CTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGC        >  SRR4302547.208997/1‑94 (MQ=60)
                                                                                     CTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGC        >  SRR4302547.4197798/1‑94 (MQ=60)
                                                                                     CTGAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGC        >  SRR4302547.5050992/1‑94 (MQ=60)
                                                                                       TAATATACCGGGCAAACACGCTTAACGCTTTATCGCCACATTCATGTCAATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCAT      <  SRR4302547.3670611/94‑1 (MQ=60)
                                                                                       GAATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCAT      <  SRR4302547.6605412/94‑1 (MQ=60)
                                                                                        AATATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATG     >  SRR4302547.5806305/1‑94 (MQ=60)
                                                                                          TATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGAC   <  SRR4302547.1356296/94‑1 (MQ=60)
                                                                                          TATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGAC   <  SRR4302547.4215255/94‑1 (MQ=60)
                                                                                           ATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGACA  <  SRR4302547.160158/94‑1 (MQ=60)
                                                                                           ATACCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGACA  >  SRR4302547.6005996/1‑94 (MQ=60)
                                                                                             |                                                                                           
CATCGACAGGATTCACCGACGGCACTGCAACAGCAAATTCTTCGCCGCCCATTCGCGCCACCAGCCCCTTATCACCGACAATCTTCTGAATATGCCGGGCAAACACGCTTAACACTTTATCGCCACATTCATGTCCATAGTTATCGTTAATGCTTTTGAAATAGTCGATATCAAGCAGCATGACA  >  NC_000913/1609167‑1609351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑