Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,555	C→T	G21E (GGG→GAG)	ynfL ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,555	0	C	T	80.1%	419.6 / 86.6	176	G21E (GGG→GAG)	ynfL	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base C (4/31); new base T (29/112); total (33/143)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 179 total aligned reads displayed.

GCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATA  >  NC_000913/1667465‑1667647

gCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCg                                                                                            <  1:5925800/93‑1 (MQ=255)

gCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCg                                                                                            <  1:4988622/93‑1 (MQ=255)

 ccAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGAGGCGCGCTCGa                                                                                           >  1:1450344/1‑93 (MQ=255)

    gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAAt                                                                                        <  2:2106959/93‑1 (MQ=255)

    gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAAt                                                                                        <  1:1918339/93‑1 (MQ=255)

    gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAAt                                                                                        <  2:5008998/93‑1 (MQ=255)

      agTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGc                                                                                      <  2:3573324/93‑1 (MQ=255)

       gTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCa                                                                                     >  2:253133/1‑93 (MQ=255)

       gTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCa                                                                                     >  1:2404775/1‑93 (MQ=255)

        tCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAg                                                                                    >  2:782626/1‑93 (MQ=255)

        tCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAg                                                                                    <  2:426125/93‑1 (MQ=255)

           gccACCAATTTGTTCCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGcac                                                                                 <  1:908985/91‑3 (MQ=255)

              aCCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTc                                                                              <  1:4323640/93‑1 (MQ=255)

              aCCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTc                                                                              <  2:3337039/93‑1 (MQ=255)

               ccAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTcc                                                                             >  1:1167618/1‑93 (MQ=255)

                cactttGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCg                                                                            <  1:3744483/90‑1 (MQ=255)

                cAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCg                                                                            >  2:3116149/1‑93 (MQ=255)

                 aaTTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgc                                                                           >  2:5785592/1‑93 (MQ=255)

                  aTTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgca                                                                          >  2:4053125/1‑93 (MQ=255)

                  aTTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgca                                                                          <  1:1993854/93‑1 (MQ=255)

                    ttgttgCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgcaac                                                                        <  2:1100942/93‑1 (MQ=255)

                     tgttgCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgcaaca                                                                       <  2:594988/93‑1 (MQ=255)

                       ttgCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgcaacagc                                                                     <  2:6098280/93‑1 (MQ=255)

                          cTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgcaacagcaac                                                                  <  1:4273498/93‑1 (MQ=255)

                          cTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCgcaacagcaac                                                                  <  1:3102091/93‑1 (MQ=255)

                             cAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACaaa                                                               <  1:3445991/93‑1 (MQ=255)

                              aGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAg                                                              <  2:811013/93‑1 (MQ=255)

                                 gcCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTaa                                                           <  2:2271973/93‑1 (MQ=255)

                                    tGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGc                                                        <  1:1074067/93‑1 (MQ=255)

                                    tGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGc                                                        <  1:1701474/93‑1 (MQ=255)

                                    tGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGc                                                        <  1:1242964/93‑1 (MQ=255)

                                     gAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCa                                                       <  2:395520/93‑1 (MQ=255)

                                     gAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCa                                                       <  2:4089798/93‑1 (MQ=255)

                                      aaTCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAg                                                      <  1:5827980/93‑1 (MQ=255)

                                       aTCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGa                                                     <  1:5380357/93‑1 (MQ=255)

                                        tCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGAt                                                    >  1:6394662/1‑93 (MQ=255)

                                        tCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGAt                                                    <  1:4585985/93‑1 (MQ=255)

                                        tCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGAt                                                    <  1:2240011/93‑1 (MQ=255)

                                         ctgctgACTTAGCGGCGGTAGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATg                                                   <  1:3310852/93‑1 (MQ=255)

                                          tgctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGa                                                  <  2:2713825/93‑1 (MQ=255)

                                          tgctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGa                                                  <  2:5631788/93‑1 (MQ=255)

                                           gctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGAc                                                 <  2:3657503/93‑1 (MQ=255)

                                           gctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGAc                                                 <  2:1806797/93‑1 (MQ=255)

                                            ctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACg                                                >  2:2852326/1‑93 (MQ=255)

                                            ctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACg                                                <  1:4917941/93‑1 (MQ=255)

                                             tgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGa                                               <  2:4874433/93‑1 (MQ=255)

                                             tgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGa                                               <  2:5185696/93‑1 (MQ=255)

                                              gACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGaa                                              <  1:707840/93‑1 (MQ=255)

                                               aCTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAg                                             <  1:4038669/93‑1 (MQ=255)

                                                cTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCGACAGCAACAAAGTAACGCAGATGACGAAGt                                            >  2:628715/1‑93 (MQ=255)

                                                cTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGt                                            <  1:91120/93‑1 (MQ=255)

                                                 ttAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGtt                                           <  2:5012921/93‑1 (MQ=255)

                                                 ttAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGtt                                           <  1:505258/93‑1 (MQ=255)

                                                 ttAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGtt                                           <  2:311192/93‑1 (MQ=255)

                                                 ttAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGtt                                           <  1:813625/93‑1 (MQ=255)

                                                  tAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTc                                          >  2:1198100/1‑93 (MQ=255)

                                                   aGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCa                                         <  1:1964163/93‑1 (MQ=255)

                                                    gcggcgGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCaa                                        <  2:1364527/93‑1 (MQ=255)

                                                    gcggcgGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCaa                                        <  2:1948808/93‑1 (MQ=255)

                                                      ggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAata                                      <  1:5888679/93‑1 (MQ=255)

                                                      ggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAata                                      <  2:3054566/93‑1 (MQ=255)

                                                       gcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAatat                                     >  1:1746671/1‑93 (MQ=255)

                                                        cggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATAtt                                    >  1:4153092/1‑93 (MQ=255)

                                                          gTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCa                                  >  1:2633617/1‑93 (MQ=255)

                                                          gTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCa                                  <  1:516286/93‑1 (MQ=255)

                                                           ttGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCat                                 >  1:1642924/1‑93 (MQ=255)

                                                            tGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCata                                <  2:6316482/93‑1 (MQ=255)

                                                             gCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCatat                               <  1:6462656/93‑1 (MQ=255)

                                                             gCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCatat                               <  1:4501929/93‑1 (MQ=255)

                                                              cGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATAtt                              <  2:265138/93‑1 (MQ=255)

                                                               gAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATAttt                             <  1:2314214/93‑1 (MQ=255)

                                                                 aaTATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTaa                           >  2:2820881/1‑93 (MQ=255)

                                                                 aaTATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTaa                           <  1:973472/93‑1 (MQ=255)

                                                                  atatTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTaaa                          <  2:2682725/93‑1 (MQ=255)

                                                                   tatTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTaaaa                         <  2:5270807/93‑1 (MQ=255)

                                                                   tatTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTaaaa                         <  2:5527192/93‑1 (MQ=255)

                                                                   tatTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTaaaa                         <  1:5753132/93‑1 (MQ=255)

                                                                     ttCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACa                       <  1:5195561/93‑1 (MQ=255)

                                                                     ttCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACa                       <  2:620374/93‑1 (MQ=255)

                                                                      tCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACAt                      <  1:2928055/93‑1 (MQ=255)

                                                                       cAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATc                     <  1:2009760/93‑1 (MQ=255)

                                                                       cAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATc                     <  1:5617342/93‑1 (MQ=255)

                                                                        aGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCt                    <  2:3344839/93‑1 (MQ=255)

                                                                        aGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCt                    <  1:245697/93‑1 (MQ=255)

                                                                         ggCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCtt                   <  2:1377411/93‑1 (MQ=255)

                                                                         ggCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCtt                   <  2:5222194/93‑1 (MQ=255)

                                                                          gCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTa                  <  2:409160/93‑1 (MQ=255)

                                                                          gCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTa                  <  1:5546207/93‑1 (MQ=255)

                                                                          gCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTa                  <  2:2739224/93‑1 (MQ=255)

                                                                           cGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAt                 <  1:3213292/93‑1 (MQ=255)

                                                                           cGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAt                 <  1:3477770/93‑1 (MQ=255)

                                                                            gggCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAtt                <  1:6605718/93‑1 (MQ=255)

                                                                             ggCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAttt               <  1:253133/93‑1 (MQ=255)

                                                                             ggCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAttt               <  1:2689278/93‑1 (MQ=255)

                                                                             ggCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAttt               <  2:3675071/93‑1 (MQ=255)

                                                                               cAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTga             <  1:5407491/93‑1 (MQ=255)

                                                                                aGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTgag            <  1:1028376/93‑1 (MQ=255)

                                                                                  cggcgCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGat          <  2:4566479/93‑1 (MQ=255)

                                                                                      gcgcCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGattatt      <  1:6105948/93‑1 (MQ=255)

                                                                                          ccGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAata  <  1:3444770/93‑1 (MQ=255)

GCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATA  >  NC_000913/1667465‑1667647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATAT  >  NC_000913/1667463‑1667648

GTGCCAGCAGTCGGGCACCAATTTGTTACTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGGAGCGGCGCGCAC                                                                                              <  SRR4302547.4228380/94‑1 (MQ=60)

  GCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGA                                                                                            <  SRR4302547.6048644/94‑1 (MQ=60)

  GCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGA                                                                                            <  SRR4302547.5091750/94‑1 (MQ=60)

  GCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGAGGCGCGCTCGA                                                                                            >  SRR4302547.1479353/1‑94 (MQ=60)

     AGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAAT                                                                                         <  SRR4302547.5971541/94‑1 (MQ=60)

      GCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATG                                                                                        <  SRR4302547.1956331/94‑1 (MQ=60)

        AGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCA                                                                                      >  SRR4302547.2453468/1‑94 (MQ=60)

           CGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCT                                                                                   <  SRR4302547.3930714/94‑1 (MQ=60)

             GCCACCAATTTGTTCCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCACT                                                                                 <  SRR4302547.926497/94‑1 (MQ=60)

               CACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTC                                                                               >  SRR4302547.6021088/1‑94 (MQ=60)

                cacaattttgttgcccaggcgctgaatccgcttgcttaggcGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCC                                                                              <  SRR4302547.449051/53‑1 (MQ=60)

                ACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCC                                                                              <  SRR4302547.4412643/94‑1 (MQ=60)

                ACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCC                                                                              >  SRR4302547.1191234/1‑94 (MQ=60)

                  CACTTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGC                                                                            <  SRR4302547.3820912/94‑1 (MQ=60)

                  CAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGC                                                                            >  SRR4302547.1748489/1‑94 (MQ=60)

                  CAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGC                                                                            >  SRR4302547.854513/1‑94 (MQ=60)

                    ATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAA                                                                          <  SRR4302547.2033507/94‑1 (MQ=60)

                       TGTTGCTCCAGCGCCTGAATCTGCTGACTTATCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAG                                                                       <  SRR4302547.3608708/94‑1 (MQ=60)

                         TTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCA                                                                     <  SRR4302547.6473010/94‑1 (MQ=60)

                         TTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCA                                                                     <  SRR4302547.1830976/94‑1 (MQ=60)

                          TGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAA                                                                    <  SRR4302547.4823644/94‑1 (MQ=60)

                            CTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACA                                                                  <  SRR4302547.3163566/94‑1 (MQ=60)

                            CTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACA                                                                  <  SRR4302547.4361239/94‑1 (MQ=60)

                               CAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAG                                                               <  SRR4302547.3516244/94‑1 (MQ=60)

                                 GCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTA                                                             <  SRR4302547.2130917/94‑1 (MQ=60)

                                  CGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAA                                                            <  SRR4302547.6131451/94‑1 (MQ=60)

                                   GCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAAC                                                           <  SRR4302547.941965/94‑1 (MQ=60)

                                   GCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAAC                                                           <  SRR4302547.5435722/94‑1 (MQ=60)

                                      TGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCA                                                        <  SRR4302547.1095498/94‑1 (MQ=60)

                                      TGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCA                                                        >  SRR4302547.1119289/1‑94 (MQ=60)

                                      TGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCA                                                        <  SRR4302547.1734911/94‑1 (MQ=60)

                                      TGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCA                                                        <  SRR4302547.1268051/94‑1 (MQ=60)

                                       GAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAG                                                       <  SRR4302547.1376698/94‑1 (MQ=60)

                                        AATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGA                                                      <  SRR4302547.5948589/94‑1 (MQ=60)

                                         ATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGAT                                                     <  SRR4302547.5491440/94‑1 (MQ=60)

                                         ATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGAT                                                     >  SRR4302547.6528064/1‑94 (MQ=60)

                                          TCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATG                                                    <  SRR4302547.2285182/94‑1 (MQ=60)

                                          TCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATG                                                    <  SRR4302547.712252/94‑1 (MQ=60)

                                          TCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATG                                                    <  SRR4302547.4680895/94‑1 (MQ=60)

                                           CTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGA                                                   <  SRR4302547.5140934/94‑1 (MQ=60)

                                           CTGCTGACTTAGCGGCGGTAGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGA                                                   <  SRR4302547.3377610/94‑1 (MQ=60)

                                              CTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGA                                                <  SRR4302547.5019802/94‑1 (MQ=60)

                                               TGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAA                                               <  SRR4302547.2038322/94‑1 (MQ=60)

                                               TGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAA                                               <  SRR4302547.2735183/94‑1 (MQ=60)

                                                GACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAG                                              <  SRR4302547.720931/94‑1 (MQ=60)

                                                 ACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGT                                             >  SRR4302547.2437586/1‑94 (MQ=60)

                                                 ACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGT                                             <  SRR4302547.4120679/94‑1 (MQ=60)

                                                 ACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGT                                             >  SRR4302547.3457028/1‑94 (MQ=60)

                                                  CTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTT                                            <  SRR4302547.92813/94‑1 (MQ=60)

                                                   TTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTC                                           <  SRR4302547.514440/94‑1 (MQ=60)

                                                   TTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTC                                           <  SRR4302547.829074/94‑1 (MQ=60)

                                                    TAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCA                                          <  SRR4302547.4164990/94‑1 (MQ=60)

                                                     AGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAA                                         <  SRR4302547.2003159/94‑1 (MQ=60)

                                                      GCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT                                        <  SRR4302547.5301478/94‑1 (MQ=60)

                                                       CGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATA                                       <  SRR4302547.3334669/94‑1 (MQ=60)

                                                        GGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATAT                                      <  SRR4302547.6010588/94‑1 (MQ=60)

                                                        GGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATAT                                      >  SRR4302547.1781121/1‑94 (MQ=60)

                                                         GCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATT                                     >  SRR4302547.4237691/1‑94 (MQ=60)

                                                          CGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTC                                    <  SRR4302547.461590/94‑1 (MQ=60)

                                                           GGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCA                                   >  SRR4302547.2686667/1‑94 (MQ=60)

                                                            GTTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCAT                                  >  SRR4302547.1675385/1‑94 (MQ=60)

                                                            GTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCAT                                  <  SRR4302547.525650/94‑1 (MQ=60)

                                                             TTGCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATA                                 <  SRR4302547.567975/94‑1 (MQ=60)

                                                               GCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATT                               <  SRR4302547.4594907/94‑1 (MQ=60)

                                                               GCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATT                               <  SRR4302547.6597234/94‑1 (MQ=60)

                                                               GCGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATT                               >  SRR4302547.5176839/1‑94 (MQ=60)

                                                                CGAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTT                              >  SRR4302547.1700854/1‑94 (MQ=60)

                                                                 GAAATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTA                             <  SRR4302547.2361127/94‑1 (MQ=60)

                                                                   AATATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAA                           <  SRR4302547.992399/94‑1 (MQ=60)

                                                                     TATTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAAC                         <  SRR4302547.5872066/94‑1 (MQ=60)

                                                                       TTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACAT                       <  SRR4302547.5302487/94‑1 (MQ=60)

                                                                       TTCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACAT                       <  SRR4302547.6641617/94‑1 (MQ=60)

                                                                       TTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACAT                       <  SRR4302547.4381032/94‑1 (MQ=60)

                                                                        TCAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATC                      <  SRR4302547.2986440/94‑1 (MQ=60)

                                                                        TCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATC                      <  SRR4302547.5857341/94‑1 (MQ=60)

                                                                         CAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCT                     <  SRR4302547.2049754/94‑1 (MQ=60)

                                                                         CAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCT                     <  SRR4302547.4479747/94‑1 (MQ=60)

                                                                         CAGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCT                     <  SRR4302547.5733261/94‑1 (MQ=60)

                                                                          AGGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTT                    <  SRR4302547.250287/94‑1 (MQ=60)

                                                                           GGCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTA                   >  SRR4302547.818784/1‑94 (MQ=60)

                                                                            GCGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTAT                  <  SRR4302547.5660645/94‑1 (MQ=60)

                                                                             CGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATT                 <  SRR4302547.3548708/94‑1 (MQ=60)

                                                                             CGGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATT                 <  SRR4302547.3277391/94‑1 (MQ=60)

                                                                              GGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTT                >  SRR4302547.1983988/1‑94 (MQ=60)

                                                                              GGGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTT                <  SRR4302547.6743227/94‑1 (MQ=60)

                                                                               GGCAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTG               <  SRR4302547.257870/94‑1 (MQ=60)

                                                                               GGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTG               <  SRR4302547.2743264/94‑1 (MQ=60)

                                                                                GCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCGTATTTAAAACATCTTATTTGA              <  SRR4302547.2350079/94‑1 (MQ=60)

                                                                                GCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGA              <  SRR4302547.2531040/94‑1 (MQ=60)

                                                                                 CAGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAG             <  SRR4302547.5519293/94‑1 (MQ=60)

                                                                                  AGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGA            <  SRR4302547.1211789/94‑1 (MQ=60)

                                                                                  AGCGGCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGA            <  SRR4302547.1048576/94‑1 (MQ=60)

                                                                                  AGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGA            <  SRR4302547.3175678/94‑1 (MQ=60)

                                                                                  AGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGA            <  SRR4302547.2884099/94‑1 (MQ=60)

                                                                                      GCGCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTAT        >  SRR4302547.5594261/1‑94 (MQ=60)

                                                                                        GCGCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTA      <  SRR4302547.3521984/94‑1 (MQ=60)

                                                                                        GCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTA      <  SRR4302547.6232605/94‑1 (MQ=60)

                                                                                          GCTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAAT    >  SRR4302547.5892791/1‑94 (MQ=60)

                                                                                           CTCGAAATGTAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATA   <  SRR4302547.1619995/94‑1 (MQ=60)

                                                                                           CTCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATA   <  SRR4302547.2298945/94‑1 (MQ=60)

                                                                                            TCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATAT  >  SRR4302547.3568484/1‑94 (MQ=60)

                                                                                            CCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATAT  <  SRR4302547.3514998/94‑1 (MQ=60)

GTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCATATTTAAAACATCTTATTTGAGATTATTAATAT  >  NC_000913/1667463‑1667648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`