Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,820	T→C	L216P (CTG→CCG)	rstA →	response transcriptional regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,820	0	T	C	86.1%	552.6 / 77.1	215	L216P (CTG→CCG)	rstA	response transcriptional regulatory protein
Reads supporting (aligned to +/- strand): ref base T (15/15); new base C (78/107); total (93/122)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-03

Only 100 of 222 total aligned reads displayed.

CGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAA  >  NC_000913/1680729‑1680912

cgcgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTg                                                                                             <  1:481149/93‑1 (MQ=255)

 gcgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGCCGTGGCTATTTGGCGGTTAAGAAAAAAACTGc                                                                                            >  1:1141527/1‑93 (MQ=255)

 gcgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGc                                                                                            <  1:827957/93‑1 (MQ=255)

 gcgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGc                                                                                            <  1:4479952/93‑1 (MQ=255)

   gATGCATTGCTGAAAAATTTACGCGGCGTCGGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTc                                                                                          >  1:5604622/1‑93 (MQ=255)

   gATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTc                                                                                          >  1:10007/1‑93 (MQ=255)

   gATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTc                                                                                          >  2:4238885/1‑93 (MQ=255)

    aTGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCg                                                                                         >  1:3028384/1‑93 (MQ=255)

      gCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGAt                                                                                       <  1:3940993/93‑1 (MQ=255)

      gCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGAt                                                                                       <  2:5083512/93‑1 (MQ=255)

       cATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATa                                                                                      >  1:4799765/1‑93 (MQ=255)

       cATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATa                                                                                      <  2:6270003/93‑1 (MQ=255)

       cATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATa                                                                                      <  2:6052104/93‑1 (MQ=255)

       cATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATa                                                                                      <  1:3714341/93‑1 (MQ=255)

        aTTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATaa                                                                                     >  2:6301069/1‑93 (MQ=255)

         ttGCTGAAAAATTTGCGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAc                                                                                    <  2:2164015/93‑1 (MQ=255)

          tGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcg                                                                                   <  2:6600063/93‑1 (MQ=255)

           gCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgc                                                                                  >  2:1524577/1‑93 (MQ=255)

           gCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgc                                                                                  >  1:4370266/1‑93 (MQ=255)

            cTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgcc                                                                                 <  2:4501076/93‑1 (MQ=255)

            cTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgcc                                                                                 <  2:863905/93‑1 (MQ=255)

            cTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgcc                                                                                 <  1:2674858/93‑1 (MQ=255)

            cTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGCTAAGAAAAAAACCGCTCGATAAcgcc                                                                                 >  2:1243486/1‑93 (MQ=255)

             tGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgccg                                                                                >  2:1749683/1‑93 (MQ=255)

             tGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAAcgccg                                                                                <  2:2717202/93‑1 (MQ=255)

               aaaaaTTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCa                                                                              <  2:4790792/93‑1 (MQ=255)

               aaaaaTTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCa                                                                              >  1:2001384/1‑93 (MQ=255)

                    tttACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAAcc                                                                         <  1:2492911/93‑1 (MQ=255)

                     ttACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGGTAACGCCGCAGAACCt                                                                        >  1:2729794/1‑93 (MQ=255)

                      tACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCtt                                                                       <  2:6002965/93‑1 (MQ=255)

                       aCGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTa                                                                      <  2:2008515/93‑1 (MQ=255)

                        cgcgGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTAt                                                                     <  2:309592/93‑1 (MQ=255)

                         gcggcgTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGGTATTTCGCGGTTAAGAAAAAAACCGCTCGATACCGCCGCAGAACCTTATc                                                                    <  2:3739573/93‑1 (MQ=255)

                         gcggcgTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATc                                                                    >  2:5268017/1‑93 (MQ=255)

                          cggcgTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTCAGAAAAAAACCGCTCGATAACGCCGCAGACCCTCATCg                                                                   >  1:505980/1‑93 (MQ=255)

                           ggcgTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGc                                                                  <  1:6249266/93‑1 (MQ=255)

                                 aGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTaaa                                                            <  1:6427264/93‑1 (MQ=255)

                                  gTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTaaaa                                                           >  2:2306326/1‑93 (MQ=255)

                                  gTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTaaaa                                                           >  1:539065/1‑93 (MQ=255)

                                   ttATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAAc                                                          >  1:4143604/1‑93 (MQ=255)

                                      tGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACtgt                                                       <  1:3975187/93‑1 (MQ=255)

                                      tGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACtgt                                                       <  2:6518893/93‑1 (MQ=255)

                                       gACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACtgtg                                                      <  2:5572891/93‑1 (MQ=255)

                                       gACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACtgtg                                                      >  2:1872302/1‑93 (MQ=255)

                                        aCGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGc                                                     <  2:887229/93‑1 (MQ=255)

                                         cGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCg                                                    >  1:2865607/1‑93 (MQ=255)

                                          ggACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGt                                                   >  1:4607791/1‑93 (MQ=255)

                                              tGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACa                                               >  2:5009207/1‑93 (MQ=255)

                                              tGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACa                                               >  2:2658296/1‑93 (MQ=255)

                                                gATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACaaa                                             <  1:5889173/93‑1 (MQ=255)

                                                 aTCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAg                                            <  2:1340405/93‑1 (MQ=255)

                                                  tCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAgg                                           <  1:6450193/93‑1 (MQ=255)

                                                  tCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAgg                                           >  1:5742363/1‑93 (MQ=255)

                                                   cGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGc                                          <  2:6561468/93‑1 (MQ=255)

                                                     tAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTa                                        <  2:2574343/93‑1 (MQ=255)

                                                      aGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTAt                                       <  1:6283995/93‑1 (MQ=255)

                                                       gCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATc                                      <  1:1779620/93‑1 (MQ=255)

                                                       gCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATc                                      <  2:1428603/93‑1 (MQ=255)

                                                        cGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCt                                     >  2:725603/1‑93 (MQ=255)

                                                         gTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCtt                                    <  2:2708625/93‑1 (MQ=255)

                                                          tGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCttt                                   >  2:6197577/1‑93 (MQ=255)

                                                          tGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCttt                                   <  1:1631862/93‑1 (MQ=255)

                                                            gACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCttttt                                 >  1:2219822/1‑93 (MQ=255)

                                                             gcGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTg                                <  2:5232102/92‑1 (MQ=255)

                                                             aCGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTg                                >  2:3846544/1‑93 (MQ=255)

                                                              cGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgc                               <  1:1191903/93‑1 (MQ=255)

                                                              cGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgc                               <  1:4921155/93‑1 (MQ=255)

                                                               gTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgcg                              >  2:1613092/1‑93 (MQ=255)

                                                               gTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgcg                              >  2:5380235/1‑93 (MQ=255)

                                                               gTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgcg                              >  1:3449414/1‑93 (MQ=255)

                                                                tGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgcgc                             >  1:5643678/1‑93 (MQ=255)

                                                                 ggCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGcc                            <  2:4799765/93‑1 (MQ=255)

                                                                   cTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTc                          >  2:1044252/1‑93 (MQ=255)

                                                                    tATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTca                         <  2:1183633/93‑1 (MQ=255)

                                                                    tATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTca                         >  2:5736350/1‑93 (MQ=255)

                                                                     aTTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcat                        <  2:758773/93‑1 (MQ=255)

                                                                     aTTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcat                        >  2:2939651/1‑93 (MQ=255)

                                                                     aTTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcat                        >  1:2740967/1‑93 (MQ=255)

                                                                       ttCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgc                      <  2:350136/93‑1 (MQ=255)

                                                                       ttCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgc                      <  2:4618854/93‑1 (MQ=255)

                                                                        tCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgca                     <  1:3750429/93‑1 (MQ=255)

                                                                         cgcgGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgcat                    <  2:4492439/93‑1 (MQ=255)

                                                                          gcgGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgcatg                   >  1:2695685/1‑93 (MQ=255)

                                                                          gcgGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgcatg                   <  1:3137715/93‑1 (MQ=255)

                                                                            ggTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATggg                 >  2:350883/1‑93 (MQ=255)

                                                                            ggTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATggg                 >  2:417526/1‑93 (MQ=255)

                                                                            ggTGAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCGATCTTTTTGCGCCTCATGCATggg                 <  2:4011893/93‑1 (MQ=255)

                                                                               tAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAAt              <  2:3903772/93‑1 (MQ=255)

                                                                               tAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCATAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAAt              <  1:4707285/93‑1 (MQ=255)

                                                                                aaGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATa             <  1:5186465/93‑1 (MQ=255)

                                                                                 aGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATaa            <  2:5876233/93‑1 (MQ=255)

                                                                                 aGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATaa            <  1:3251221/93‑1 (MQ=255)

                                                                                 aGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATaa            <  2:3277059/93‑1 (MQ=255)

                                                                                   aaaaaaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGc          <  1:3790890/93‑1 (MQ=255)

                                                                                   aaaaaaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGAATGGGAATAAGc          >  1:5275259/1‑93 (MQ=255)

                                                                                    aaaaaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCg         >  1:1450882/1‑93 (MQ=255)

                                                                                     aaaaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGa        <  2:1907297/93‑1 (MQ=255)

                                                                                     aaaaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGa        <  1:1906360/93‑1 (MQ=255)

                                                                                     aaaaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGa        <  1:5837434/93‑1 (MQ=255)

                                                                                       aaaCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATg      <  1:3582847/93‑1 (MQ=255)

CGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAA  >  NC_000913/1680729‑1680912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAA  >  NC_000913/1680727‑1680911

GCCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACC                                                                                             <  SRR4302547.5688897/94‑1 (MQ=60)

ACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACC                                                                                             <  SRR4302547.6694872/94‑1 (MQ=60)

ACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACC                                                                                             >  SRR4302547.614217/1‑94 (MQ=60)

ACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACC                                                                                             <  SRR4302547.3857497/94‑1 (MQ=60)

ACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACC                                                                                             <  SRR4302547.6388317/94‑1 (MQ=60)

  CGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGCCGTGGCTATTTGGCGGTTAAGAAAAAAACTGC                                                                                           >  SRR4302547.1164586/1‑94 (MQ=60)

  CGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGC                                                                                           <  SRR4302547.489979/94‑1 (MQ=60)

   GCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCT                                                                                          <  SRR4302547.843764/94‑1 (MQ=60)

   GCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCT                                                                                          <  SRR4302547.3376823/94‑1 (MQ=60)

   GCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCT                                                                                          >  SRR4302547.2985659/1‑94 (MQ=60)

   GCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCT                                                                                          <  SRR4302547.271239/94‑1 (MQ=60)

   GCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCT                                                                                          <  SRR4302547.4572506/94‑1 (MQ=60)

    CGATGCATTGCTGAAAAATTTACGCGGCGTCGGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTC                                                                                         >  SRR4302547.5720247/1‑94 (MQ=60)

    CGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTC                                                                                         <  SRR4302547.4888445/94‑1 (MQ=60)

    CGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTC                                                                                         <  SRR4302547.746086/94‑1 (MQ=60)

    CGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTC                                                                                         >  SRR4302547.1169782/1‑94 (MQ=60)

    CGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTC                                                                                         >  SRR4302547.10193/1‑94 (MQ=60)

     GATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCG                                                                                        >  SRR4302547.3883569/1‑94 (MQ=60)

     GATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCG                                                                                        >  SRR4302547.4555951/1‑94 (MQ=60)

     GATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCG                                                                                        >  SRR4302547.3088600/1‑94 (MQ=60)

      ATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGA                                                                                       >  SRR4302547.3143118/1‑94 (MQ=60)

      ATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGA                                                                                       <  SRR4302547.5970329/94‑1 (MQ=60)

       CGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGAT                                                                                      >  SRR4302547.6772647/1‑94 (MQ=60)

        GCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATA                                                                                     >  SRR4302547.4899223/1‑94 (MQ=60)

        GCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATA                                                                                     <  SRR4302547.4021135/94‑1 (MQ=60)

         CATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAA                                                                                    <  SRR4302547.3790160/94‑1 (MQ=60)

          ATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAca                                                                                   >  SRR4302547.2804540/1‑92 (MQ=60)

            TGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGC                                                                                 >  SRR4302547.4460048/1‑94 (MQ=60)

              CTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCG                                                                               <  SRR4302547.2728585/94‑1 (MQ=60)

               TGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGC                                                                              >  SRR4302547.6017798/1‑94 (MQ=60)

                GAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCA                                                                             >  SRR4302547.2041212/1‑94 (MQ=60)

                 AAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAG                                                                            <  SRR4302547.2788733/94‑1 (MQ=60)

                      TTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGGTAACGCCGCAGAACCT                                                                       >  SRR4302547.2784587/1‑94 (MQ=60)

                      TTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCT                                                                       <  SRR4302547.2543259/94‑1 (MQ=60)

                       TTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTT                                                                      <  SRR4302547.2619846/94‑1 (MQ=60)

                       TTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTT                                                                      <  SRR4302547.4312572/94‑1 (MQ=60)

                         ACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTAT                                                                    <  SRR4302547.3948893/94‑1 (MQ=60)

                           GCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTCAGAAAAAAACCGCTCGATAACGCCGCAGACCCTCATCG                                                                  >  SRR4302547.515171/1‑94 (MQ=60)

                            CGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGC                                                                 >  SRR4302547.1769354/1‑94 (MQ=60)

                             GGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCA                                                                >  SRR4302547.1935505/1‑94 (MQ=60)

                             GGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCA                                                                <  SRR4302547.6379085/94‑1 (MQ=60)

                               CGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATT                                                              <  SRR4302547.5193001/94‑1 (MQ=60)

                                GTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTA                                                             <  SRR4302547.5432011/94‑1 (MQ=60)

                                   AGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAA                                                          <  SRR4302547.6561258/94‑1 (MQ=60)

                                   AGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAA                                                          >  SRR4302547.548799/1‑94 (MQ=60)

                                   AGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAA                                                          >  SRR4302547.3258875/1‑94 (MQ=60)

                                    GTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAAC                                                         >  SRR4302547.4938792/1‑94 (MQ=60)

                                    GTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAAC                                                         >  SRR4302547.4227932/1‑94 (MQ=60)

                                       ATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACCGT                                                      <  SRR4302547.1578080/94‑1 (MQ=60)

                                        TGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTG                                                     <  SRR4302547.4056023/94‑1 (MQ=60)

                                          ACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCG                                                   >  SRR4302547.2922847/1‑94 (MQ=60)

                                           CGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGT                                                  <  SRR4302547.5741419/94‑1 (MQ=60)

                                           CGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGT                                                  >  SRR4302547.4703151/1‑94 (MQ=60)

                                              ACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAAC                                               >  SRR4302547.4938005/1‑94 (MQ=60)

                                                  GATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAG                                           <  SRR4302547.6011092/94‑1 (MQ=60)

                                                   ATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGG                                          >  SRR4302547.5861030/1‑94 (MQ=60)

                                                    TCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGC                                         <  SRR4302547.6584570/94‑1 (MQ=60)

                                                     CGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCT                                        <  SRR4302547.3797337/94‑1 (MQ=60)

                                                     CGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCT                                        <  SRR4302547.401815/94‑1 (MQ=60)

                                                        AGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATC                                     <  SRR4302547.6414555/94‑1 (MQ=60)

                                                        AGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATC                                     >  SRR4302547.1056592/1‑94 (MQ=60)

                                                         GCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCT                                    <  SRR4302547.1814894/94‑1 (MQ=60)

                                                         GCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCT                                    <  SRR4302547.2768713/94‑1 (MQ=60)

                                                            TGGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTT                                 <  SRR4302547.1664162/94‑1 (MQ=60)

                                                             GGACGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTT                                >  SRR4302547.2264541/1‑94 (MQ=60)

                                                                CGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCG                             >  SRR4302547.2071853/1‑94 (MQ=60)

                                                                CGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCG                             <  SRR4302547.1215956/94‑1 (MQ=60)

                                                                CGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCG                             <  SRR4302547.5023074/94‑1 (MQ=60)

                                                                CGTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCG                             >  SRR4302547.3519726/1‑94 (MQ=60)

                                                                 GTGGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGC                            >  SRR4302547.5760171/1‑94 (MQ=60)

                                                                   GGCTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCT                          <  SRR4302547.5302888/94‑1 (MQ=60)

                                                                     CTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCA                        >  SRR4302547.6387448/1‑94 (MQ=60)

                                                                     CTATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCA                        <  SRR4302547.1364175/94‑1 (MQ=60)

                                                                      TATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCAT                       >  SRR4302547.2795955/1‑94 (MQ=60)

                                                                       ATTTCGCGGTTAAGAAAAAACCCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATG                      <  SRR4302547.4744888/94‑1 (MQ=60)

                                                                       ATTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGATCCTTATCGCATTAAATCTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATG                      <  SRR4302547.4288658/94‑1 (MQ=60)

                                                                        TTTCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGC                     <  SRR4302547.983265/94‑1 (MQ=60)

                                                                          TCGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCAT                   <  SRR4302547.3826975/94‑1 (MQ=60)

                                                                           CGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATG                  >  SRR4302547.2749803/1‑94 (MQ=60)

                                                                           CGCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATG                  <  SRR4302547.3827008/94‑1 (MQ=60)

                                                                            GCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGG                 <  SRR4302547.6431449/94‑1 (MQ=60)

                                                                            GCGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGG                 <  SRR4302547.3200031/94‑1 (MQ=60)

                                                                             CGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGG                <  SRR4302547.4909752/94‑1 (MQ=60)

                                                                             CGGTTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGG                >  SRR4302547.4689750/1‑94 (MQ=60)

                                                                             CGGTTAAGAAAAAAAACGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGG                >  SRR4302547.4136046/1‑94 (MQ=60)

                                                                              GGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGA               >  SRR4302547.583852/1‑94 (MQ=60)

                                                                                TTAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAAT             >  SRR4302547.1782539/1‑94 (MQ=60)

                                                                                TGAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCGCATGCATGGGAAT             <  SRR4302547.2266617/94‑1 (MQ=60)

                                                                                 TAAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCATAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATA            <  SRR4302547.4804766/94‑1 (MQ=60)

                                                                                  AAGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAA           <  SRR4302547.5293209/94‑1 (MQ=60)

                                                                                   AGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAG          >  SRR4302547.5888977/1‑94 (MQ=60)

                                                                                   AGAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAG          <  SRR4302547.3316177/94‑1 (MQ=60)

                                                                                    GAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGC         <  SRR4302547.4693481/94‑1 (MQ=60)

                                                                                    GAAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGAATGGGAATAAGC         >  SRR4302547.5384002/1‑94 (MQ=60)

                                                                                     AAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCG        <  SRR4302547.1952379/94‑1 (MQ=60)

                                                                                     AAAAAAACCTCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCG        <  SRR4302547.567347/94‑1 (MQ=60)

                                                                                     AAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCG        >  SRR4302547.1479900/1‑94 (MQ=60)

                                                                                     AAAAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCG        <  SRR4302547.3868184/94‑1 (MQ=60)

                                                                                       AAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGAT      >  SRR4302547.5977055/1‑94 (MQ=60)

                                                                                       AAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGAT      <  SRR4302547.1944086/94‑1 (MQ=60)

                                                                                       AAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGAT      <  SRR4302547.6578382/94‑1 (MQ=60)

                                                                                       AAAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGAT      <  SRR4302547.5958215/94‑1 (MQ=60)

                                                                                        AAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATG     <  SRR4302547.5529111/94‑1 (MQ=60)

                                                                                        AAAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATG     <  SRR4302547.3630686/94‑1 (MQ=60)

                                                                                         AAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGA    <  SRR4302547.3655948/94‑1 (MQ=60)

                                                                                         AAACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGA    >  SRR4302547.2898385/1‑94 (MQ=60)

                                                                                          AACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAA   <  SRR4302547.273232/94‑1 (MQ=60)

                                                                                          AACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAA   <  SRR4302547.5102775/94‑1 (MQ=60)

                                                                                           ACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAA  <  SRR4302547.3778978/94‑1 (MQ=60)

                                                                                           ACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAA  >  SRR4302547.3642282/1‑94 (MQ=60)

                                                                                           ACCGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAA  >  SRR4302547.1742690/1‑94 (MQ=60)

ACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAA  >  NC_000913/1680727‑1680911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`