Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,667	T→C	intergenic (‑502/‑55)	b1675 ← / → pykF	hypothetical protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,667	0	T	C	82.2%	460.5 / 94.0	198	intergenic (‑502/‑55)	b1675/pykF	hypothetical protein/pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (18/17); new base C (75/87); total (94/104)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 208 total aligned reads displayed.

GCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA  >  NC_000913/1753577‑1753758

gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAgcgc                                                                                           >  1:3549640/1‑93 (MQ=255)

gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAgcgc                                                                                           >  2:1170297/1‑93 (MQ=255)

 cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGcgcg                                                                                          <  1:5146068/93‑1 (MQ=255)

     caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCa                                                                                      <  1:3481699/93‑1 (MQ=255)

     caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCa                                                                                      <  1:5271146/93‑1 (MQ=255)

      aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAg                                                                                     <  1:2013036/93‑1 (MQ=255)

        tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGaa                                                                                   <  1:1795720/93‑1 (MQ=255)

         ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGaaa                                                                                  <  1:4817508/93‑1 (MQ=255)

          ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAg                                                                                 >  2:603109/1‑93 (MQ=255)

           tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTGGCTTTCCGGCAGCGCCCCCAGAAAga                                                                                >  2:2385226/1‑92 (MQ=255)

           tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAga                                                                                >  1:6405067/1‑92 (MQ=255)

           tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGc                                                                                <  1:1542055/93‑1 (MQ=255)

            ctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCa                                                                               >  1:3050027/1‑93 (MQ=255)

            ctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCa                                                                               >  2:6122089/1‑93 (MQ=255)

             tGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCaa                                                                              <  2:1136200/93‑1 (MQ=255)

               cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGt                                                                            <  1:2585084/93‑1 (MQ=255)

                aCGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGtt                                                                           <  2:2535655/93‑1 (MQ=255)

                  gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTtc                                                                         <  1:4860881/93‑1 (MQ=255)

                  gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTtc                                                                         <  2:1887277/93‑1 (MQ=255)

                    tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTtctc                                                                       >  2:3421384/1‑93 (MQ=255)

                    tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTtctc                                                                       >  2:3012769/1‑93 (MQ=255)

                     ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTcc                                                                      <  2:4760300/93‑1 (MQ=255)

                     ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTcc                                                                      <  2:374937/93‑1 (MQ=255)

                     ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTcc                                                                      >  2:4224247/1‑93 (MQ=255)

                       ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCa                                                                    <  1:2106402/93‑1 (MQ=255)

                        tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCAt                                                                   <  2:3455253/93‑1 (MQ=255)

                        tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCAt                                                                   <  2:5981477/93‑1 (MQ=255)

                          gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                 <  2:2938063/93‑1 (MQ=255)

                          gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                 <  1:1481464/93‑1 (MQ=255)

                          gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                 >  1:795961/1‑93 (MQ=255)

                          gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                 <  1:5386760/93‑1 (MQ=255)

                           aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                >  2:2505079/1‑93 (MQ=255)

                            tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCtt                                                               <  1:6146210/93‑1 (MQ=255)

                              tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                             >  1:3406491/1‑93 (MQ=255)

                               cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc                                                            >  2:2752531/1‑93 (MQ=255)

                                aCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                           >  1:4778068/1‑93 (MQ=255)

                                 ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa                                                          >  1:4941218/1‑93 (MQ=255)

                                 ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa                                                          <  1:4130690/93‑1 (MQ=255)

                                  cTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGAGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaat                                                         >  1:4385930/1‑92 (MQ=255)

                                   tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                        >  1:5455728/1‑93 (MQ=255)

                                   tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCATCt                                                        >  2:1014740/1‑93 (MQ=255)

                                   tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                        >  1:5856366/1‑93 (MQ=255)

                                    aTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACtt                                                       >  2:6655436/1‑93 (MQ=255)

                                     tCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa                                                      >  1:3656576/1‑93 (MQ=255)

                                     tCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa                                                      >  1:418134/1‑93 (MQ=255)

                                     tCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa                                                      <  2:1720745/93‑1 (MQ=255)

                                       cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaaa                                                    <  1:6323517/93‑1 (MQ=255)

                                       cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaaa                                                    >  2:6099502/1‑93 (MQ=255)

                                        ttAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                   >  2:4653624/1‑93 (MQ=255)

                                         tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaaga                                                  >  2:3371593/1‑93 (MQ=255)

                                           gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagact                                                <  2:853425/93‑1 (MQ=255)

                                            agCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagacta                                               <  2:2825416/93‑1 (MQ=255)

                                             gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              >  1:4221416/1‑93 (MQ=255)

                                             gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              <  1:5965538/93‑1 (MQ=255)

                                             gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              >  1:5794180/1‑93 (MQ=255)

                                               gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTCaagactaaga                                            >  1:5024736/1‑93 (MQ=255)

                                               gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                            <  1:5592026/93‑1 (MQ=255)

                                               gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                            <  1:279712/93‑1 (MQ=255)

                                               gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                            <  1:569907/93‑1 (MQ=255)

                                                aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                           <  1:3864937/93‑1 (MQ=255)

                                                aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                           <  1:5179252/93‑1 (MQ=255)

                                                  gCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                         <  2:2203069/93‑1 (MQ=255)

                                                  gCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                         <  1:4543029/93‑1 (MQ=255)

                                                   caccaccacTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGt                                        <  1:1152815/93‑1 (MQ=255)

                                                   caccaccacTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGt                                        <  1:3359335/93‑1 (MQ=255)

                                                      caccacTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                     <  2:1656489/93‑1 (MQ=255)

                                                       accacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                    <  2:2087183/93‑1 (MQ=255)

                                                       accacTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                    >  1:1075017/1‑93 (MQ=255)

                                                       accacTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                    >  2:119529/1‑93 (MQ=255)

                                                               cGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            >  2:6387139/1‑93 (MQ=255)

                                                                gTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAcc                           <  1:3252258/93‑1 (MQ=255)

                                                                  aaTACCGGATTCGCTTTCCGGCAGCGCGCCCGGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                         <  2:2673793/93‑1 (MQ=255)

                                                                  aaTACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                         <  2:1370558/93‑1 (MQ=255)

                                                                   aTACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa                        <  2:145066/93‑1 (MQ=255)

                                                                   aTACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGTCCaaa                        >  1:4722641/1‑93 (MQ=255)

                                                                   aTACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa                        >  2:5418894/1‑93 (MQ=255)

                                                                    tACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaaa                       >  1:754288/1‑93 (MQ=255)

                                                                      ccGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAtt                     <  2:5080076/93‑1 (MQ=255)

                                                                      ccGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAtt                     <  2:5220158/93‑1 (MQ=255)

                                                                       cGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                    <  2:6170219/93‑1 (MQ=255)

                                                                       cGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                    >  1:2209994/1‑93 (MQ=255)

                                                                         gATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                  >  2:3573298/1‑93 (MQ=255)

                                                                         gATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                  <  2:5609986/93‑1 (MQ=255)

                                                                          aTTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 >  2:6476673/1‑93 (MQ=255)

                                                                          aTTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:3938348/93‑1 (MQ=255)

                                                                          aTTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:4010291/93‑1 (MQ=255)

                                                                           ttCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                <  1:6040024/93‑1 (MQ=255)

                                                                            tCGCTTTCCGGCAGCGCGCACAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc               <  1:6385537/93‑1 (MQ=255)

                                                                              gCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc             >  2:735558/1‑93 (MQ=255)

                                                                               cTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc            >  1:4374798/1‑93 (MQ=255)

                                                                                tttCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCa           >  1:4242322/1‑93 (MQ=255)

                                                                                tttCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCa           >  1:884838/1‑93 (MQ=255)

                                                                                 ttCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCAt          <  2:1531025/93‑1 (MQ=255)

                                                                                  tCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc         <  1:3533477/93‑1 (MQ=255)

                                                                                  tCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc         <  1:2306751/93‑1 (MQ=255)

                                                                                   ccGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCg        <  1:4646886/93‑1 (MQ=255)

                                                                                    cGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg       >  2:4318302/1‑93 (MQ=255)

                                                                                        aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCg   >  2:2598633/1‑93 (MQ=255)

                                                                                        aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCg   >  1:1403737/1‑93 (MQ=255)

                                                                                         gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGa  >  2:1009753/1‑93 (MQ=255)

GCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA  >  NC_000913/1753577‑1753758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCG  >  NC_000913/1753574‑1753757

CAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGC                                                                                            >  SRR4302547.2305189/1‑94 (MQ=60)

CAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGC                                                                                            <  SRR4302547.5776300/94‑1 (MQ=60)

 AAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCG                                                                                           >  SRR4302547.147936/1‑94 (MQ=60)

 AAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCG                                                                                           >  SRR4302547.2804158/1‑94 (MQ=60)

  AGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGC                                                                                          >  SRR4302547.2190966/1‑94 (MQ=60)

  AGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGC                                                                                          >  SRR4302547.3622083/1‑94 (MQ=60)

  AGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGC                                                                                          <  SRR4302547.642675/94‑1 (MQ=60)

  AGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGC                                                                                          <  SRR4302547.1419266/94‑1 (MQ=60)

  AGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGC                                                                                          <  SRR4302547.4065959/94‑1 (MQ=60)

   GCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCG                                                                                         <  SRR4302547.5432882/94‑1 (MQ=60)

    CACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGC                                                                                        >  SRR4302547.434377/1‑94 (MQ=60)

    CACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGC                                                                                        <  SRR4302547.5252058/94‑1 (MQ=60)

     ACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCC                                                                                       <  SRR4302547.6232453/94‑1 (MQ=60)

        CATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAG                                                                                    <  SRR4302547.5379764/94‑1 (MQ=60)

        CATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAG                                                                                    <  SRR4302547.3552725/94‑1 (MQ=60)

         ATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGA                                                                                   <  SRR4302547.2053085/94‑1 (MQ=60)

           TCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAA                                                                                 <  SRR4302547.1831248/94‑1 (MQ=60)

            CCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAG                                                                                <  SRR4302547.4917347/94‑1 (MQ=60)

            CCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAG                                                                                <  SRR4302547.869418/94‑1 (MQ=60)

             CTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGA                                                                               >  SRR4302547.6538651/1‑94 (MQ=60)

             CTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGC                                                                               <  SRR4302547.5729234/94‑1 (MQ=60)

              TCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCA                                                                              <  SRR4302547.1572748/94‑1 (MQ=60)

              TCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCA                                                                              >  SRR4302547.3110707/1‑94 (MQ=60)

               CTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAA                                                                             >  SRR4302547.6406189/1‑94 (MQ=60)

               CTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAA                                                                             <  SRR4302547.6143256/94‑1 (MQ=60)

                TGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAG                                                                            >  SRR4302547.3354807/1‑94 (MQ=60)

                TGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCACGCCCAGAAAGCAAG                                                                            >  SRR4302547.1707905/1‑94 (MQ=60)

                 GCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGT                                                                           >  SRR4302547.4469236/1‑94 (MQ=60)

                  CACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTT                                                                          <  SRR4302547.2637246/94‑1 (MQ=60)

                   ACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTT                                                                         >  SRR4302547.1520329/1‑94 (MQ=60)

                     GCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCT                                                                       <  SRR4302547.4961611/94‑1 (MQ=60)

                      CTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTC                                                                      >  SRR4302547.1102200/1‑94 (MQ=60)

                         TTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCA                                                                   <  SRR4302547.360938/94‑1 (MQ=60)

                          TTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCAT                                                                  <  SRR4302547.5174384/94‑1 (MQ=60)

                          TTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCAT                                                                  <  SRR4302547.2148371/94‑1 (MQ=60)

                            CGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCC                                                                >  SRR4302547.811030/1‑94 (MQ=60)

                             GATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCT                                                               <  SRR4302547.5497993/94‑1 (MQ=60)

                             GATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCT                                                               <  SRR4302547.1511044/94‑1 (MQ=60)

                              ATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTT                                                              >  SRR4302547.943140/1‑94 (MQ=60)

                               TGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTC                                                             <  SRR4302547.6273730/94‑1 (MQ=60)

                                GTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCT                                                            >  SRR4302547.3475805/1‑94 (MQ=60)

                                  CACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCA                                                          >  SRR4302547.4877044/1‑94 (MQ=60)

                                  CACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCA                                                          <  SRR4302547.2001168/94‑1 (MQ=60)

                                   ACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTTCCATCCTTCTCAA                                                         >  SRR4302547.4023720/1‑94 (MQ=60)

                                   ACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAA                                                         >  SRR4302547.5043541/1‑94 (MQ=60)

                                    CCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAC                                                        >  SRR4302547.695899/1‑94 (MQ=60)

                                    CCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAC                                                        <  SRR4302547.4214726/94‑1 (MQ=60)

                                    CCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGAGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAT                                                        >  SRR4302547.4476163/1‑94 (MQ=60)

                                     CTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACT                                                       >  SRR4302547.5568450/1‑94 (MQ=60)

                                     CTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACT                                                       >  SRR4302547.4819486/1‑94 (MQ=60)

                                     CTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACT                                                       <  SRR4302547.421136/94‑1 (MQ=60)

                                     CTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACT                                                       >  SRR4302547.5977546/1‑94 (MQ=60)

                                       ATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTA                                                     >  SRR4302547.425886/1‑94 (MQ=60)

                                       ATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTA                                                     >  SRR4302547.3731175/1‑94 (MQ=60)

                                        TCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAA                                                    <  SRR4302547.4279745/94‑1 (MQ=60)

                                          CTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAG                                                  <  SRR4302547.6455002/94‑1 (MQ=60)

                                          CTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAG                                                  >  SRR4302547.2079994/1‑94 (MQ=60)

                                          CTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAG                                                  >  SRR4302547.4932875/1‑94 (MQ=60)

                                          CTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAG                                                  <  SRR4302547.5463931/94‑1 (MQ=60)

                                            TAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGAC                                                <  SRR4302547.650584/94‑1 (MQ=60)

                                             AGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACT                                               <  SRR4302547.6747173/94‑1 (MQ=60)

                                             AGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACT                                               <  SRR4302547.5909128/94‑1 (MQ=60)

                                             AGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACT                                               <  SRR4302547.3247323/94‑1 (MQ=60)

                                               AGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAA                                             >  SRR4302547.4310550/1‑94 (MQ=60)

                                               AGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAA                                             >  SRR4302547.4307636/1‑94 (MQ=60)

                                               AGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAA                                             >  SRR4302547.5914037/1‑94 (MQ=60)

                                               AGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTGCTCCCATCCTTCTCAACTTAAAGACTAA                                             <  SRR4302547.3561822/94‑1 (MQ=60)

                                                GCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAG                                            <  SRR4302547.6089262/94‑1 (MQ=60)

                                                GCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAG                                            <  SRR4302547.1259666/94‑1 (MQ=60)

                                                GCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAG                                            <  SRR4302547.6478851/94‑1 (MQ=60)

                                                 CGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTCAAGACTAAGA                                           >  SRR4302547.5128554/1‑94 (MQ=60)

                                                 CGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGA                                           >  SRR4302547.5261346/1‑94 (MQ=60)

                                                  GAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGAC                                          <  SRR4302547.580153/94‑1 (MQ=60)

                                                  GAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGAC                                          <  SRR4302547.284928/94‑1 (MQ=60)

                                                  GAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGAC                                          <  SRR4302547.5707387/94‑1 (MQ=60)

                                                   AGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACT                                         <  SRR4302547.3943575/94‑1 (MQ=60)

                                                   AGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACT                                         <  SRR4302547.5285854/94‑1 (MQ=60)

                                                   AGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACT                                         <  SRR4302547.3661327/94‑1 (MQ=60)

                                                     GCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGT                                       <  SRR4302547.4636954/94‑1 (MQ=60)

                                                      CACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTC                                      >  SRR4302547.3543307/1‑94 (MQ=60)

                                                      CACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTC                                      <  SRR4302547.1176147/94‑1 (MQ=60)

                                                      CACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTC                                      <  SRR4302547.3427497/94‑1 (MQ=60)

                                                       ACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCA                                     >  SRR4302547.4405469/1‑94 (MQ=60)

                                                       ACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCA                                     >  SRR4302547.5511531/1‑94 (MQ=60)

                                                         CACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG                                   <  SRR4302547.5922813/94‑1 (MQ=60)

                                                         CACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG                                   >  SRR4302547.1096473/1‑94 (MQ=60)

                                                         CACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG                                   <  SRR4302547.2476049/94‑1 (MQ=60)

                                                            CACTTTCGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAA                                >  SRR4302547.6069131/1‑94 (MQ=60)

                                                                  CGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACC                          <  SRR4302547.5082698/94‑1 (MQ=60)

                                                                  CGTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACC                          >  SRR4302547.4481076/1‑94 (MQ=60)

                                                                   GTAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCA                         <  SRR4302547.3317238/94‑1 (MQ=60)

                                                                    TAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAA                        >  SRR4302547.5293644/1‑94 (MQ=60)

                                                                    TAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAA                        <  SRR4302547.4903937/94‑1 (MQ=60)

                                                                    TAATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAA                        >  SRR4302547.4662811/1‑94 (MQ=60)

                                                                     AATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGTCCAAA                       >  SRR4302547.4820430/1‑94 (MQ=60)

                                                                      ATACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAA                      >  SRR4302547.768505/1‑94 (MQ=60)

                                                                       TACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAT                     >  SRR4302547.5042794/1‑94 (MQ=60)

                                                                       TACCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAT                     <  SRR4302547.3792262/94‑1 (MQ=60)

                                                                         CCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTG                   >  SRR4302547.2254428/1‑94 (MQ=60)

                                                                         CCGGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTG                   <  SRR4302547.3269302/94‑1 (MQ=60)

                                                                           GGATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTT                 >  SRR4302547.2346365/1‑94 (MQ=60)

                                                                            GATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTT                <  SRR4302547.4859298/94‑1 (MQ=60)

                                                                             ATTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTG               <  SRR4302547.5636633/94‑1 (MQ=60)

                                                                              TTCGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGC              <  SRR4302547.6165359/94‑1 (MQ=60)

                                                                               TCGCTTTCCGGCAGCGCGCACAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCA             <  SRR4302547.6518784/94‑1 (MQ=60)

                                                                                CGCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAC            >  SRR4302547.1459849/1‑94 (MQ=60)

                                                                                 GCTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACC           >  SRR4302547.4464699/1‑94 (MQ=60)

                                                                                  CTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCA          <  SRR4302547.2985789/94‑1 (MQ=60)

                                                                                  CTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCA          >  SRR4302547.4329080/1‑94 (MQ=60)

                                                                                  CTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCA          >  SRR4302547.3439125/1‑94 (MQ=60)

                                                                                  CTTTCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCA          >  SRR4302547.901839/1‑94 (MQ=60)

                                                                                     TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCG       <  SRR4302547.2806581/94‑1 (MQ=60)

                                                                                     TCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCG       <  SRR4302547.3605632/94‑1 (MQ=60)

                                                                                     TCCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCG       <  SRR4302547.2353510/94‑1 (MQ=60)

                                                                                      CCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGG      <  SRR4302547.4743147/94‑1 (MQ=60)

                                                                                      CCGGCAGCGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGG      >  SRR4302547.800924/1‑94 (MQ=60)

                                                                                          CAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCG  >  SRR4302547.1431819/1‑94 (MQ=60)

CAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCG  >  NC_000913/1753574‑1753757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`