Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,763,263 T→C T132A (ACG→GCG)  b1686 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,2630TC89.9% 594.1 / 49.8 208T132A (ACG→GCG) b1686hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/10);  new base C (98/88);  total (110/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

Only 100 of 223 total aligned reads displayed.

TGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCA  >  NC_000913/1763172‑1763353
                                                                                           |                                                                                          
 gCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCa                                                                                          <  1:2927337/93‑1 (MQ=255)
  cacaGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCaa                                                                                         <  2:4602866/93‑1 (MQ=255)
  cacaGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCaa                                                                                         <  2:2956173/93‑1 (MQ=255)
   acaGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAAt                                                                                        <  1:5533575/93‑1 (MQ=255)
    caGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATc                                                                                       <  1:4043935/93‑1 (MQ=255)
    caGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATc                                                                                       <  1:5167924/93‑1 (MQ=255)
    caGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATc                                                                                       >  2:2352742/1‑93 (MQ=255)
     aGTATACCACCGTTTACTGTGCAGATAATGACCGAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCg                                                                                      <  2:2024686/93‑1 (MQ=255)
     aGTATACCACCGTTTACTATGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCg                                                                                      <  1:4309862/93‑1 (MQ=255)
       tataCCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTg                                                                                    >  2:3266075/1‑93 (MQ=255)
        ataCCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGa                                                                                   >  1:3855517/1‑93 (MQ=255)
          accaccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACg                                                                                 <  2:1913033/93‑1 (MQ=255)
          accaccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACg                                                                                 >  2:28409/1‑93 (MQ=255)
           ccaccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGa                                                                                >  2:1846857/1‑93 (MQ=255)
            caccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGaa                                                                               <  1:6030977/93‑1 (MQ=255)
            caccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGaa                                                                               >  2:5118059/1‑93 (MQ=255)
            caccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGaa                                                                               >  2:760822/1‑93 (MQ=255)
             accGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAc                                                                              >  1:188222/1‑93 (MQ=255)
             accGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAc                                                                              >  1:2739565/1‑93 (MQ=255)
             accGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAc                                                                              <  1:5304780/93‑1 (MQ=255)
             accGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAc                                                                              >  2:4082529/1‑93 (MQ=255)
             accGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAc                                                                              >  2:5168620/1‑93 (MQ=255)
                gTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGc                                                                           >  1:1165551/1‑93 (MQ=255)
                     cTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACaaa                                                                      <  2:662793/93‑1 (MQ=255)
                     cTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACaaa                                                                      <  1:1697886/93‑1 (MQ=255)
                     cTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACaaa                                                                      <  2:3098891/93‑1 (MQ=255)
                      tgtgCAGATAATGACCAAACGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAAc                                                                     >  1:6459081/1‑93 (MQ=255)
                       gtgCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACg                                                                    >  2:6100771/1‑93 (MQ=255)
                         gCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCCGCAATCCTGACGAACAGCACAAACGcc                                                                  >  1:5360756/1‑93 (MQ=255)
                          cAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGccc                                                                 >  2:5816023/1‑93 (MQ=255)
                          cAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGccc                                                                 >  1:342961/1‑93 (MQ=255)
                          cAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGccc                                                                 >  1:3057845/1‑93 (MQ=255)
                           aGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCt                                                                <  1:3329496/93‑1 (MQ=255)
                             aTAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCttt                                                              >  1:337347/1‑93 (MQ=255)
                              tAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTtc                                                             <  2:4842346/93‑1 (MQ=255)
                              tAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTCACGAACAGCACAAACGCCCTTtc                                                             <  1:5972705/93‑1 (MQ=255)
                               aaTGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTtct                                                            >  1:370281/1‑93 (MQ=255)
                               aaTGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTtct                                                            <  1:5732285/93‑1 (MQ=255)
                                 tGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTAACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCa                                                          >  2:4229022/1‑93 (MQ=255)
                                  gACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                                                         >  2:4143386/1‑93 (MQ=255)
                                  gACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                                                         >  2:388536/1‑93 (MQ=255)
                                  gACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                                                         >  2:6202240/1‑93 (MQ=255)
                                  gACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                                                         <  2:6583006/93‑1 (MQ=255)
                                     cAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGa                                                      >  1:5762376/1‑93 (MQ=255)
                                       aaaGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAg                                                    >  2:4970326/1‑93 (MQ=255)
                                       aaaGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAg                                                    >  2:1090643/1‑93 (MQ=255)
                                        aaGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGa                                                   >  1:1016695/1‑93 (MQ=255)
                                        aaGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGa                                                   <  1:4655352/93‑1 (MQ=255)
                                         aGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAt                                                  <  2:4739070/93‑1 (MQ=255)
                                         aGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAt                                                  >  2:135926/1‑93 (MQ=255)
                                           cAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAttt                                                <  1:2395676/93‑1 (MQ=255)
                                              tatGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCaa                                             >  2:4207140/1‑93 (MQ=255)
                                                tGCGTCACAGTTTTCTGTTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATc                                           <  1:4757021/93‑1 (MQ=255)
                                                  cGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTg                                         <  2:5054906/93‑1 (MQ=255)
                                                   gTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGc                                        >  2:2367609/1‑93 (MQ=255)
                                                    tCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGcc                                       <  1:1425119/93‑1 (MQ=255)
                                                     cacaCTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCa                                      >  2:5487628/1‑93 (MQ=255)
                                                     cacaCTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCa                                      >  2:2152967/1‑93 (MQ=255)
                                                        acTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGAc                                   <  1:4842295/93‑1 (MQ=255)
                                                          ttttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCt                                 >  1:2333529/1‑93 (MQ=255)
                                                          ttttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCt                                 <  2:6095820/93‑1 (MQ=255)
                                                           tttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTg                                >  1:440091/1‑93 (MQ=255)
                                                           tttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTg                                >  1:631541/1‑93 (MQ=255)
                                                           tttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTg                                <  1:2975239/93‑1 (MQ=255)
                                                            ttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTgg                               <  2:5701010/93‑1 (MQ=255)
                                                            ttCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTgg                               <  2:4384432/93‑1 (MQ=255)
                                                             tCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGt                              <  1:2631894/93‑1 (MQ=255)
                                                             tCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGt                              <  2:2767348/93‑1 (MQ=255)
                                                             tCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGt                              >  2:3606540/1‑93 (MQ=255)
                                                                ggTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAc                           >  2:444279/1‑93 (MQ=255)
                                                                  tGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcgc                         <  1:157133/93‑1 (MQ=255)
                                                                   gACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcgcg                        >  1:1215608/1‑93 (MQ=255)
                                                                   gACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcgcg                        <  2:4076266/93‑1 (MQ=255)
                                                                   gACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcgcg                        <  1:2149751/93‑1 (MQ=255)
                                                                    aCAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGa                       >  2:4660238/1‑93 (MQ=255)
                                                                    aCAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGa                       >  2:1621985/1‑93 (MQ=255)
                                                                    aCAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGa                       >  1:2831485/1‑93 (MQ=255)
                                                                     cAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGaa                      <  1:2159510/93‑1 (MQ=255)
                                                                     cAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGaa                      >  2:2627791/1‑93 (MQ=255)
                                                                     cAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCGCATCGAAGATTTCAATCTGCCAGCCCTGGTGACGCGaa                      <  1:5080415/93‑1 (MQ=255)
                                                                      aaCGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAAc                     >  2:3285172/1‑93 (MQ=255)
                                                                      aaCGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAAc                     <  2:142501/93‑1 (MQ=255)
                                                                       aCGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAAcc                    >  1:1362654/1‑93 (MQ=255)
                                                                       aCGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAAcc                    >  1:2527561/1‑93 (MQ=255)
                                                                          tCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGGCGCGAACCgag                 >  1:1621389/1‑93 (MQ=255)
                                                                          tCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCgag                 >  1:6583004/1‑93 (MQ=255)
                                                                           cacaAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCgaga                <  1:4518321/93‑1 (MQ=255)
                                                                            acaAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGAt               <  1:3569669/93‑1 (MQ=255)
                                                                             caAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATg              <  2:5029521/93‑1 (MQ=255)
                                                                             caAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATg              >  2:71004/1‑93 (MQ=255)
                                                                              aaaaTGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGc             <  1:6530518/93‑1 (MQ=255)
                                                                               aaaTGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCa            >  2:5326839/1‑93 (MQ=255)
                                                                                 aTGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAAc          <  1:3203500/93‑1 (MQ=255)
                                                                                   ggCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACgg        <  1:3903946/93‑1 (MQ=255)
                                                                                       gtcgccAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTg    >  2:5339536/1‑93 (MQ=255)
                                                                                       gtcgccAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTg    >  1:5251076/1‑93 (MQ=255)
                                                                                       gtcgccAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTg    >  1:4261382/1‑93 (MQ=255)
                                                                                       gtcgccAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTg    >  1:3955523/1‑93 (MQ=255)
                                                                                       gtcgccAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTg    >  1:6240897/1‑93 (MQ=255)
                                                                                         cgtcAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCa  <  1:1322219/93‑1 (MQ=255)
                                                                                           |                                                                                          
TGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCA  >  NC_000913/1763172‑1763353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCATAC  >  NC_000913/1763170‑1763356
                                                                                             |                                                                                             
CCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGT                                                                                               <  SRR4302547.2984904/94‑1 (MQ=60)
CCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGC                                                                                               >  SRR4302547.121786/1‑94 (MQ=60)
CCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGC                                                                                               >  SRR4302547.1333086/1‑94 (MQ=60)
  TGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCA                                                                                             >  SRR4302547.5525622/1‑94 (MQ=60)
   GCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAA                                                                                            <  SRR4302547.2985713/94‑1 (MQ=60)
     ACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATC                                                                                          <  SRR4302547.5647779/94‑1 (MQ=60)
      CAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCG                                                                                         <  SRR4302547.3848259/94‑1 (MQ=60)
      CAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCG                                                                                         <  SRR4302547.5274328/94‑1 (MQ=60)
      CAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCG                                                                                         <  SRR4302547.4126049/94‑1 (MQ=60)
       AGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGT                                                                                        <  SRR4302547.2921503/94‑1 (MQ=60)
       AGTATACCACCGTTTACTATGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGT                                                                                        <  SRR4302547.4398520/94‑1 (MQ=60)
        GTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTG                                                                                       >  SRR4302547.6213760/1‑94 (MQ=60)
         TATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGA                                                                                      <  SRR4302547.4700719/94‑1 (MQ=60)
         TATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGA                                                                                      >  SRR4302547.3933994/1‑94 (MQ=60)
           TACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACG                                                                                    >  SRR4302547.1299613/1‑94 (MQ=60)
           TACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACG                                                                                    <  SRR4302547.3183504/94‑1 (MQ=60)
           TACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACG                                                                                    >  SRR4302547.5690540/1‑94 (MQ=60)
             CCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAA                                                                                  <  SRR4302547.3865237/94‑1 (MQ=60)
             CCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAA                                                                                  >  SRR4302547.2451362/1‑94 (MQ=60)
              CACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAC                                                                                 <  SRR4302547.1709348/94‑1 (MQ=60)
              CACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAC                                                                                 >  SRR4302547.191771/1‑94 (MQ=60)
              CACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAC                                                                                 >  SRR4302547.2794528/1‑94 (MQ=60)
              CACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAAC                                                                                 <  SRR4302547.6156138/94‑1 (MQ=60)
               ACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACA                                                                                <  SRR4302547.1190770/94‑1 (MQ=60)
               ACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACA                                                                                <  SRR4302547.5414198/94‑1 (MQ=60)
               ACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACA                                                                                >  SRR4302547.2332220/1‑94 (MQ=60)
                 CGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGC                                                                              >  SRR4302547.1189128/1‑94 (MQ=60)
                   TTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCAC                                                                            <  SRR4302547.1748824/94‑1 (MQ=60)
                       CTGTGCAGATAATGACCAAACGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAAC                                                                        >  SRR4302547.6593598/1‑94 (MQ=60)
                       CTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAAC                                                                        >  SRR4302547.658464/1‑94 (MQ=60)
                       CTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAAC                                                                        <  SRR4302547.1436309/94‑1 (MQ=60)
                       CTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAAC                                                                        <  SRR4302547.1731270/94‑1 (MQ=60)
                       CTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGGGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAAC                                                                        >  SRR4302547.3478421/1‑94 (MQ=60)
                        TGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACG                                                                       <  SRR4302547.2418734/94‑1 (MQ=60)
                          TGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCC                                                                     >  SRR4302547.6178274/1‑94 (MQ=60)
                          TGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCCGCAATCCTGACGAACAGCACAAACGCC                                                                     >  SRR4302547.5471333/1‑94 (MQ=60)
                           GCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCC                                                                    >  SRR4302547.3118691/1‑94 (MQ=60)
                           GCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCC                                                                    >  SRR4302547.349336/1‑94 (MQ=60)
                           GCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCC                                                                    <  SRR4302547.5305442/94‑1 (MQ=60)
                            CAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCT                                                                   >  SRR4302547.3303629/1‑94 (MQ=60)
                            CAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCT                                                                   >  SRR4302547.6551459/1‑94 (MQ=60)
                            CAGATAATGACCAAAAGCAATATGCGCCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCT                                                                   >  SRR4302547.3416101/1‑94 (MQ=60)
                             AGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTT                                                                  <  SRR4302547.3396820/94‑1 (MQ=60)
                             AGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTT                                                                  <  SRR4302547.1428086/94‑1 (MQ=60)
                             AGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTT                                                                  <  SRR4302547.5321579/94‑1 (MQ=60)
                              GATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTT                                                                 >  SRR4302547.343622/1‑94 (MQ=60)
                               ATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTC                                                                >  SRR4302547.4019634/1‑94 (MQ=60)
                                TAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCT                                                               >  SRR4302547.377141/1‑94 (MQ=60)
                                TAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCT                                                               <  SRR4302547.3976890/94‑1 (MQ=60)
                                TAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTCACGAACAGCACAAACGCCCTTTCT                                                               <  SRR4302547.6096585/94‑1 (MQ=60)
                                 AATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTC                                                              <  SRR4302547.2951331/94‑1 (MQ=60)
                                 AATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTC                                                              >  SRR4302547.6425141/1‑94 (MQ=60)
                                 AATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTC                                                              <  SRR4302547.5850717/94‑1 (MQ=60)
                                   TGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCAT                                                            >  SRR4302547.1554373/1‑94 (MQ=60)
                                     ACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCG                                                          <  SRR4302547.135471/94‑1 (MQ=60)
                                     ACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACACACGCCCTTTCTCATCG                                                          >  SRR4302547.2698739/1‑94 (MQ=60)
                                     ACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCG                                                          >  SRR4302547.4574660/1‑94 (MQ=60)
                                      CCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGA                                                         >  SRR4302547.5881550/1‑94 (MQ=60)
                                         AAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGA                                                      >  SRR4302547.1036604/1‑94 (MQ=60)
                                         AAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCACTTTCTCATCGAAGA                                                      >  SRR4302547.3569081/1‑94 (MQ=60)
                                          AAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAT                                                     >  SRR4302547.4924730/1‑94 (MQ=60)
                                          AAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAT                                                     >  SRR4302547.2678588/1‑94 (MQ=60)
                                          AAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAT                                                     <  SRR4302547.4751768/94‑1 (MQ=60)
                                          AAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAT                                                     <  SRR4302547.3104395/94‑1 (MQ=60)
                                             CAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTC                                                  <  SRR4302547.2444202/94‑1 (MQ=60)
                                             CAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTC                                                  >  SRR4302547.1627070/1‑94 (MQ=60)
                                              AATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCA                                                 >  SRR4302547.5258207/1‑94 (MQ=60)
                                              AATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCA                                                 <  SRR4302547.6143601/94‑1 (MQ=60)
                                               ATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAA                                                >  SRR4302547.2192945/1‑94 (MQ=60)
                                               ATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAA                                                >  SRR4302547.4251422/1‑94 (MQ=60)
                                                  TGCGTCACAGTTTTCTGTTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCT                                             <  SRR4302547.4855586/94‑1 (MQ=60)
                                                  TGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCT                                             <  SRR4302547.5458698/94‑1 (MQ=60)
                                                  TGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCT                                             <  SRR4302547.4309917/94‑1 (MQ=60)
                                                   GCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTG                                            <  SRR4302547.580471/94‑1 (MQ=60)
                                                    CGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGC                                           >  SRR4302547.6006444/1‑94 (MQ=60)
                                                      TCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCA                                         <  SRR4302547.1453641/94‑1 (MQ=60)
                                                          ACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACC                                     <  SRR4302547.4942650/94‑1 (MQ=60)
                                                           CTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAGTCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCT                                    <  SRR4302547.6673874/94‑1 (MQ=60)
                                                           CTTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCT                                    >  SRR4302547.2380820/1‑94 (MQ=60)
                                                            TTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTG                                   >  SRR4302547.643208/1‑94 (MQ=60)
                                                            TTTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTG                                   >  SRR4302547.448291/1‑94 (MQ=60)
                                                            TTTTCTGGTGACAACGTCACAAAATGGCGGACGCCAATCGTGACGAACAGCACAAAagaccactctcatcgcagatctccatctgccccacctg                                   >  SRR4302547.448292/1‑56 (MQ=60)
                                                             TTTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGG                                  <  SRR4302547.3034445/94‑1 (MQ=60)
                                                              TTCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGT                                 <  SRR4302547.5367847/94‑1 (MQ=60)
                                                               TCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTG                                <  SRR4302547.1146080/94‑1 (MQ=60)
                                                               TCTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTG                                <  SRR4302547.2684894/94‑1 (MQ=60)
                                                                CTGGTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGA                               >  SRR4302547.3702080/1‑94 (MQ=60)
                                                                   GTGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGC                            <  SRR4302547.5871446/94‑1 (MQ=60)
                                                                    TGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCG                           <  SRR4302547.160014/94‑1 (MQ=60)
                                                                    TGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCG                           <  SRR4302547.2143292/94‑1 (MQ=60)
                                                                    TGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCG                           >  SRR4302547.1240131/1‑94 (MQ=60)
                                                                    TGACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCA                           <  SRR4302547.4651148/94‑1 (MQ=60)
                                                                     GACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGA                          >  SRR4302547.2888104/1‑94 (MQ=60)
                                                                     GACAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGA                          <  SRR4302547.2192655/94‑1 (MQ=60)
                                                                       CAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAAC                        <  SRR4302547.2202669/94‑1 (MQ=60)
                                                                       CAACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCGCATCGAAGATTTCAATCTGCCAGCCCTGGTGACGCGAAC                        <  SRR4302547.5185242/94‑1 (MQ=60)
                                                                        AACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACC                       >  SRR4302547.2578645/1‑94 (MQ=60)
                                                                        AACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACC                       >  SRR4302547.1389996/1‑94 (MQ=60)
                                                                         ACGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCG                      <  SRR4302547.947244/94‑1 (MQ=60)
                                                                          CGTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGA                     >  SRR4302547.4758843/1‑94 (MQ=60)
                                                                           GTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGGCGCGAACCGAG                    >  SRR4302547.1653474/1‑94 (MQ=60)
                                                                           GTCACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAG                    >  SRR4302547.6720081/1‑94 (MQ=60)
                                                                             CACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGAT                  <  SRR4302547.4611663/94‑1 (MQ=60)
                                                                             CACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGAT                  <  SRR4302547.3348344/94‑1 (MQ=60)
                                                                             CACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGAT                  >  SRR4302547.4377698/1‑94 (MQ=60)
                                                                              ACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATG                 <  SRR4302547.3642491/94‑1 (MQ=60)
                                                                              ACAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATG                 >  SRR4302547.1747839/1‑94 (MQ=60)
                                                                               CAAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGC                >  SRR4302547.3007491/1‑94 (MQ=60)
                                                                                AAAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCA               <  SRR4302547.6666438/94‑1 (MQ=60)
                                                                                 AAATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAA              >  SRR4302547.4409436/1‑94 (MQ=60)
                                                                                   ATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACG            <  SRR4302547.511693/94‑1 (MQ=60)
                                                                                   ATGGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACG            <  SRR4302547.3267389/94‑1 (MQ=60)
                                                                                     GGCGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGT          <  SRR4302547.3983339/94‑1 (MQ=60)
                                                                                     GGCGGTCGCCAATCGGGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTAGTGACGCGAACCGAGATGCAACGGT          >  SRR4302547.2498335/1‑94 (MQ=60)
                                                                                       CGGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTT        >  SRR4302547.2852662/1‑94 (MQ=60)
                                                                                        TGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTG       >  SRR4302547.4348590/1‑94 (MQ=60)
                                                                                        GGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTG       >  SRR4302547.6370551/1‑94 (MQ=60)
                                                                                        GGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTG       >  SRR4302547.4035982/1‑94 (MQ=60)
                                                                                        GGTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTG       >  SRR4302547.5359198/1‑94 (MQ=60)
                                                                                         GTCGCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGC      >  SRR4302547.4077793/1‑94 (MQ=60)
                                                                                           CGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCAT    <  SRR4302547.1348795/94‑1 (MQ=60)
                                                                                            GCCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCATA   >  SRR4302547.5719441/1‑94 (MQ=60)
                                                                                             CCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGCTGCAACGGTTTGCATAC  >  SRR4302547.1008375/1‑94 (MQ=60)
                                                                                             CCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCATAC  <  SRR4302547.909043/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAGATGCAACGGTTTGCATAC  >  NC_000913/1763170‑1763356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑