Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,820,066 A→G I72T (ATC→ACC)  osmE ← OsmE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,820,0660AG85.6% 614.5 / 86.8 236I72T (ATC→ACC) osmEOsmE
Reads supporting (aligned to +/- strand):  ref base A (18/16);  new base G (109/93);  total (127/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 241 total aligned reads displayed.

AGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCT  >  NC_000913/1819975‑1820158
                                                                                           |                                                                                            
aGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAt                                                                                             >  2:4477982/1‑93 (MQ=255)
 gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTg                                                                                            >  1:4395469/1‑93 (MQ=255)
 gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTg                                                                                            <  2:4819111/93‑1 (MQ=255)
 gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATg                                                                                            >  1:2124015/1‑93 (MQ=255)
   acaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTa                                                                                          <  1:5398183/93‑1 (MQ=255)
   acaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTa                                                                                          <  1:3645556/93‑1 (MQ=255)
    caGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAg                                                                                         <  2:2004849/93‑1 (MQ=255)
    caGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAg                                                                                         <  2:4844609/93‑1 (MQ=255)
     aGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAgg                                                                                        <  2:5142776/93‑1 (MQ=255)
        tCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCt                                                                                     >  2:522701/1‑93 (MQ=255)
             tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCaa                                                                                <  2:1705854/93‑1 (MQ=255)
             tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCaa                                                                                <  2:733981/93‑1 (MQ=255)
              aaCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAg                                                                               >  1:1116912/1‑93 (MQ=255)
              aaCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAg                                                                               <  2:2455987/93‑1 (MQ=255)
               aCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAga                                                                              >  2:584334/1‑92 (MQ=255)
                 cGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTAc                                                                            <  1:5038236/93‑1 (MQ=255)
                  ggAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTAcc                                                                           >  2:3721683/1‑93 (MQ=255)
                   gAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACcg                                                                          >  1:1385702/1‑93 (MQ=255)
                     gTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACcgcg                                                                        >  2:6149555/1‑93 (MQ=255)
                      ttGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGa                                                                       <  1:4694455/93‑1 (MQ=255)
                      ttGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGa                                                                       <  1:3264674/93‑1 (MQ=255)
                        gatgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGc                                                                     <  1:5614891/93‑1 (MQ=255)
                         atgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCa                                                                    <  2:717347/93‑1 (MQ=255)
                         atgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCa                                                                    <  2:85451/93‑1 (MQ=255)
                          tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGGTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCAt                                                                   >  2:227911/1‑93 (MQ=255)
                           gaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATg                                                                  >  2:133440/1‑93 (MQ=255)
                           gaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATg                                                                  <  2:2728155/93‑1 (MQ=255)
                            aCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATgg                                                                 >  1:4213591/1‑93 (MQ=255)
                             cATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGa                                                                >  1:5015799/1‑93 (MQ=255)
                             cATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGa                                                                >  1:352326/1‑93 (MQ=255)
                             cATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGa                                                                <  1:129125/93‑1 (MQ=255)
                              aTGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGAt                                                               >  1:99678/1‑93 (MQ=255)
                              aTGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGAt                                                               <  1:280816/93‑1 (MQ=255)
                                gTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                             <  1:3007431/93‑1 (MQ=255)
                                gTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                             <  1:1527417/93‑1 (MQ=255)
                                gTCCGCTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                             <  2:35289/93‑1 (MQ=255)
                                 tCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCAt                                                            >  1:6244510/1‑93 (MQ=255)
                                   cGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                          <  1:4043153/93‑1 (MQ=255)
                                   cGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                          >  2:1171017/1‑93 (MQ=255)
                                    ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCt                                                         >  2:2682368/1‑93 (MQ=255)
                                    ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCt                                                         >  1:578123/1‑93 (MQ=255)
                                      gatcagcTAACGCGACAAAGGAGGTTTCTGCTTTACCATCGCGGTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCa                                                       <  2:3251611/92‑1 (MQ=255)
                                       atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAc                                                      >  1:2620309/1‑93 (MQ=255)
                                        tcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt                                                     >  2:1415916/1‑93 (MQ=255)
                                        tcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt                                                     >  2:1019277/1‑93 (MQ=255)
                                        tcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt                                                     >  1:390667/1‑93 (MQ=255)
                                        tcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt                                                     >  2:3350450/1‑93 (MQ=255)
                                         catcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACtt                                                    <  2:1956399/93‑1 (MQ=255)
                                         catcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACtt                                                    >  1:6282496/1‑93 (MQ=255)
                                          atcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTc                                                   >  2:5619932/1‑93 (MQ=255)
                                           tcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGAAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                  >  2:1074049/1‑93 (MQ=255)
                                            cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAg                                                 >  2:4165367/1‑93 (MQ=255)
                                             tAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGa                                                <  1:277361/93‑1 (MQ=255)
                                             tAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGa                                                >  2:408422/1‑93 (MQ=255)
                                              aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                               <  2:5444719/93‑1 (MQ=255)
                                              aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                               >  2:1475421/1‑93 (MQ=255)
                                               aCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACg                                              <  1:5859845/93‑1 (MQ=255)
                                                 gcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGaa                                            <  2:3591876/93‑1 (MQ=255)
                                                  cgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTCCCGCGAGCATGGATCATGCTCACTTCAGACGAAg                                           <  1:1893827/93‑1 (MQ=255)
                                                    aCAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGt                                         >  1:4824957/1‑93 (MQ=255)
                                                     cAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                        <  1:5688739/93‑1 (MQ=255)
                                                     cAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                        >  1:256707/1‑93 (MQ=255)
                                                       aaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTa                                      >  2:3947319/1‑93 (MQ=255)
                                                       aaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCACGAGCATGTATCATGCTCACTTCAGACGAAGGTTTa                                      >  2:2903738/1‑93 (MQ=255)
                                                        aGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAc                                     <  2:1548359/93‑1 (MQ=255)
                                                        aGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAc                                     >  1:1768914/1‑93 (MQ=255)
                                                        aGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAc                                     >  1:2136599/1‑93 (MQ=255)
                                                         gTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                    >  1:2409986/1‑93 (MQ=255)
                                                         gTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                    >  1:41277/1‑93 (MQ=255)
                                                         gTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                    <  1:540377/93‑1 (MQ=255)
                                                          tAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAccc                                   <  2:5168030/93‑1 (MQ=255)
                                                           aGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                  <  2:1828519/93‑1 (MQ=255)
                                                           aGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                  >  1:5594661/1‑93 (MQ=255)
                                                           aGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                  <  2:4318926/93‑1 (MQ=255)
                                                           aGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                  >  1:5258455/1‑93 (MQ=255)
                                                              tttCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCat                               >  1:6154233/1‑92 (MQ=255)
                                                              tttCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCaa                               <  2:5467924/93‑1 (MQ=255)
                                                                tCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt                             >  1:1944762/1‑93 (MQ=255)
                                                                tCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCTGCAAtt                             >  2:3552469/1‑93 (MQ=255)
                                                                 cTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAttt                            >  1:2830926/1‑93 (MQ=255)
                                                                    cTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                         >  1:3411380/1‑93 (MQ=255)
                                                                       tACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCaa                      >  1:788415/1‑93 (MQ=255)
                                                                        aCCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc                     >  1:5131392/1‑93 (MQ=255)
                                                                        aCCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc                     >  2:5250791/1‑93 (MQ=255)
                                                                         ccATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAcc                    <  2:2461578/93‑1 (MQ=255)
                                                                           aTCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTg                  >  1:6610473/1‑93 (MQ=255)
                                                                            tCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgc                 <  1:266939/93‑1 (MQ=255)
                                                                             cACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcg                >  1:1554175/1‑93 (MQ=255)
                                                                               cGTTGACCCAGGGTGTAGGTCTGGCACGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGcc              <  2:6644538/93‑1 (MQ=255)
                                                                               cGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGcc              <  1:3593616/93‑1 (MQ=255)
                                                                                 ttGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCg            <  2:6580928/93‑1 (MQ=255)
                                                                                    aCCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCt         >  1:6041212/1‑93 (MQ=255)
                                                                                    aCCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCt         >  1:6506407/1‑93 (MQ=255)
                                                                                     cccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc        >  2:923870/1‑93 (MQ=255)
                                                                                      ccAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCa       >  1:3082139/1‑93 (MQ=255)
                                                                                      ccAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCa       >  2:1104159/1‑93 (MQ=255)
                                                                                       cAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAt      >  2:499925/1‑93 (MQ=255)
                                                                                        aGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATg     <  1:2475503/93‑1 (MQ=255)
                                                                                        aGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATg     <  2:3305924/93‑1 (MQ=255)
                                                                                          gATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGcc   >  2:5315503/1‑93 (MQ=255)
                                                                                           |                                                                                            
AGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCT  >  NC_000913/1819975‑1820158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTT  >  NC_000913/1819973‑1820159
                                                                                             |                                                                                             
TCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGG                                                                                               <  SRR4302547.6053545/94‑1 (MQ=60)
 CAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGT                                                                                              <  SRR4302547.3300229/94‑1 (MQ=60)
 CAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGT                                                                                              <  SRR4302547.5579947/94‑1 (MQ=60)
 CAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGT                                                                                              >  SRR4302547.4813975/1‑94 (MQ=60)
  AGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTG                                                                                             <  SRR4302547.6339079/94‑1 (MQ=60)
  AGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTG                                                                                             >  SRR4302547.6179328/1‑94 (MQ=60)
  AGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTG                                                                                             >  SRR4302547.4485948/1‑94 (MQ=60)
  AGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATG                                                                                             >  SRR4302547.2166346/1‑94 (MQ=60)
    CACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTA                                                                                           <  SRR4302547.1542796/94‑1 (MQ=60)
    CACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTA                                                                                           <  SRR4302547.1784258/94‑1 (MQ=60)
     ACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAG                                                                                          <  SRR4302547.2476190/94‑1 (MQ=60)
     ACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAG                                                                                          <  SRR4302547.5509733/94‑1 (MQ=60)
     ACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAG                                                                                          <  SRR4302547.1491198/94‑1 (MQ=60)
     ACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAG                                                                                          <  SRR4302547.3719915/94‑1 (MQ=60)
      CAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGG                                                                                         >  SRR4302547.1805135/1‑94 (MQ=60)
      CAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGG                                                                                         <  SRR4302547.5261228/94‑1 (MQ=60)
       AGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGT                                                                                        <  SRR4302547.5536339/94‑1 (MQ=60)
       AGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGT                                                                                        >  SRR4302547.4023620/1‑94 (MQ=60)
           CTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGG                                                                                    >  SRR4302547.4977782/1‑94 (MQ=60)
             GATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCA                                                                                  <  SRR4302547.2619969/94‑1 (MQ=60)
             GATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCA                                                                                  <  SRR4302547.6407371/94‑1 (MQ=60)
             GATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCA                                                                                  <  SRR4302547.3588270/94‑1 (MQ=60)
               TAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAG                                                                                >  SRR4302547.1139407/1‑94 (MQ=60)
                AACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGT                                                                               >  SRR4302547.2166634/1‑94 (MQ=60)
                 ACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTA                                                                              >  SRR4302547.4154405/1‑94 (MQ=60)
                 ACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTA                                                                              <  SRR4302547.4893075/94‑1 (MQ=60)
                  CCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTAC                                                                             >  SRR4302547.990460/1‑94 (MQ=60)
                   CGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACC                                                                            >  SRR4302547.5109997/1‑94 (MQ=60)
                   CGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACC                                                                            >  SRR4302547.3145157/1‑94 (MQ=60)
                   CGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACC                                                                            <  SRR4302547.4230249/94‑1 (MQ=60)
                   CGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACC                                                                            >  SRR4302547.1701482/1‑94 (MQ=60)
                   CGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACC                                                                            >  SRR4302547.1584611/1‑94 (MQ=60)
                   CGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACC                                                                            <  SRR4302547.5142289/94‑1 (MQ=60)
                    GGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCG                                                                           >  SRR4302547.1413445/1‑94 (MQ=60)
                    GGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCG                                                                           >  SRR4302547.6310829/1‑94 (MQ=60)
                      AGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCG                                                                         <  SRR4302547.3512724/94‑1 (MQ=60)
                        TTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAG                                                                       <  SRR4302547.4791687/94‑1 (MQ=60)
                        TTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAG                                                                       <  SRR4302547.3329984/94‑1 (MQ=60)
                          GATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCA                                                                     <  SRR4302547.5730763/94‑1 (MQ=60)
                          GATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCA                                                                     <  SRR4302547.1931428/94‑1 (MQ=60)
                             GACATGTCGAGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGG                                                                  >  SRR4302547.6675450/1‑94 (MQ=60)
                             GACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGG                                                                  >  SRR4302547.4299590/1‑94 (MQ=60)
                             GACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGG                                                                  >  SRR4302547.3871414/1‑94 (MQ=60)
                              ACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGA                                                                 >  SRR4302547.5119474/1‑94 (MQ=60)
                              ACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGA                                                                 >  SRR4302547.358873/1‑94 (MQ=60)
                              ACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGAGAGCATGGA                                                                 <  SRR4302547.6603645/94‑1 (MQ=60)
                               CATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGAT                                                                <  SRR4302547.131459/94‑1 (MQ=60)
                               CATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGAT                                                                >  SRR4302547.101515/1‑94 (MQ=60)
                                ATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATC                                                               <  SRR4302547.286042/94‑1 (MQ=60)
                                ATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATC                                                               <  SRR4302547.5168043/94‑1 (MQ=60)
                                 TGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCA                                                              >  SRR4302547.2846182/1‑94 (MQ=60)
                                  GTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCAT                                                             <  SRR4302547.3067238/94‑1 (MQ=60)
                                  GTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCAT                                                             >  SRR4302547.6374228/1‑94 (MQ=60)
                                  GTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCAT                                                             <  SRR4302547.1557812/94‑1 (MQ=60)
                                     CGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCT                                                          >  SRR4302547.588484/1‑94 (MQ=60)
                                     CGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCT                                                          <  SRR4302547.4125253/94‑1 (MQ=60)
                                      GGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTC                                                         >  SRR4302547.688016/1‑94 (MQ=60)
                                       GTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCA                                                        >  SRR4302547.511719/1‑94 (MQ=60)
                                        TATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAC                                                       >  SRR4302547.2673098/1‑94 (MQ=60)
                                         ATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACT                                                      >  SRR4302547.5748683/1‑94 (MQ=60)
                                         ATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACT                                                      <  SRR4302547.1342022/94‑1 (MQ=60)
                                         ATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACT                                                      >  SRR4302547.397919/1‑94 (MQ=60)
                                          TCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTT                                                     <  SRR4302547.2377034/94‑1 (MQ=60)
                                          TCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTT                                                     >  SRR4302547.6413019/1‑94 (MQ=60)
                                           CATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTC                                                    <  SRR4302547.3894103/94‑1 (MQ=60)
                                           CATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTC                                                    >  SRR4302547.2027702/1‑94 (MQ=60)
                                            ATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCA                                                   <  SRR4302547.6011919/94‑1 (MQ=60)
                                               TAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAC                                                <  SRR4302547.282538/94‑1 (MQ=60)
                                                AACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACG                                               >  SRR4302547.2790644/1‑94 (MQ=60)
                                                 ACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGA                                              <  SRR4302547.5981112/94‑1 (MQ=60)
                                                 ACGCGACAAAGTAGGCTTCTGCTTTACCATCACGTTGACCCAGGGTGAAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGA                                              >  SRR4302547.2881114/1‑94 (MQ=60)
                                                   GCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAG                                            <  SRR4302547.752239/94‑1 (MQ=60)
                                                   GCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAG                                            >  SRR4302547.4533304/1‑94 (MQ=60)
                                                    CGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTCCCGCGAGCATGGATCATGCTCACTTCAGACGAAGG                                           <  SRR4302547.1931252/94‑1 (MQ=60)
                                                    CGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGG                                           >  SRR4302547.1204965/1‑94 (MQ=60)
                                                     GACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGT                                          >  SRR4302547.4924986/1‑94 (MQ=60)
                                                     GACAAAGTAGGTTTCTGCTTTACCATCACGTTGACACAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCCGACGAAGGT                                          <  SRR4302547.6640398/94‑1 (MQ=60)
                                                      ACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTT                                         <  SRR4302547.341000/94‑1 (MQ=60)
                                                      ACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTT                                         <  SRR4302547.4389198/94‑1 (MQ=60)
                                                      ACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTT                                         >  SRR4302547.261514/1‑94 (MQ=60)
                                                       CAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTT                                        <  SRR4302547.5511583/94‑1 (MQ=60)
                                                       CAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTT                                        <  SRR4302547.5806211/94‑1 (MQ=60)
                                                        AAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTA                                       >  SRR4302547.5757761/1‑94 (MQ=60)
                                                        AAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTA                                       >  SRR4302547.3692411/1‑94 (MQ=60)
                                                         AAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAC                                      >  SRR4302547.1803991/1‑94 (MQ=60)
                                                         AAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAC                                      >  SRR4302547.2179160/1‑94 (MQ=60)
                                                          AGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACC                                     <  SRR4302547.2719645/94‑1 (MQ=60)
                                                          AGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACC                                     >  SRR4302547.42038/1‑94 (MQ=60)
                                                          AGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACC                                     >  SRR4302547.2458778/1‑94 (MQ=60)
                                                          AGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTCCCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACC                                     <  SRR4302547.5513765/94‑1 (MQ=60)
                                                           GTAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCC                                    <  SRR4302547.550131/94‑1 (MQ=60)
                                                            TAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCG                                   >  SRR4302547.5710071/1‑94 (MQ=60)
                                                            TAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCG                                   >  SRR4302547.3110908/1‑94 (MQ=60)
                                                            TAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCG                                   >  SRR4302547.5366738/1‑94 (MQ=60)
                                                            TAGGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGAATGGATCATGCTCACTTCAGACGAAGCTTTACCCA                                   >  SRR4302547.2663357/1‑94 (MQ=60)
                                                            TAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCG                                   >  SRR4302547.5921858/1‑94 (MQ=60)
                                                              GGTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCA                                 <  SRR4302547.2891713/94‑1 (MQ=60)
                                                               GTTTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAT                                >  SRR4302547.6281927/1‑94 (MQ=60)
                                                                 TTCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATT                              >  SRR4302547.1983321/1‑94 (MQ=60)
                                                                  TCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTT                             >  SRR4302547.2887535/1‑94 (MQ=60)
                                                                  TCTGCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTT                             >  SRR4302547.5921208/1‑94 (MQ=60)
                                                                     GCTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTG                          >  SRR4302547.3480827/1‑94 (MQ=60)
                                                                      CTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGC                         <  SRR4302547.615681/94‑1 (MQ=60)
                                                                      CTTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGC                         <  SRR4302547.2027977/94‑1 (MQ=60)
                                                                       TTTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCA                        >  SRR4302547.4148559/1‑94 (MQ=60)
                                                                        TTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAA                       >  SRR4302547.803338/1‑94 (MQ=60)
                                                                        TTACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAA                       >  SRR4302547.2803321/1‑94 (MQ=60)
                                                                         TACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAC                      >  SRR4302547.5237120/1‑94 (MQ=60)
                                                                          ACCATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACC                     <  SRR4302547.4778025/94‑1 (MQ=60)
                                                                          ACCATCACGCTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACC                     >  SRR4302547.581510/1‑94 (MQ=60)
                                                                            CATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTG                   >  SRR4302547.6748069/1‑94 (MQ=60)
                                                                            CATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTG                   >  SRR4302547.2529161/1‑94 (MQ=60)
                                                                             ATCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGC                  <  SRR4302547.3228704/94‑1 (MQ=60)
                                                                             ATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGC                  <  SRR4302547.5289300/94‑1 (MQ=60)
                                                                              TCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCG                 >  SRR4302547.1585103/1‑94 (MQ=60)
                                                                              TCACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCG                 <  SRR4302547.271932/94‑1 (MQ=60)
                                                                                ACGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCC               >  SRR4302547.2220294/1‑94 (MQ=60)
                                                                                 CGTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCC              <  SRR4302547.3666915/94‑1 (MQ=60)
                                                                                  GTTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCG             >  SRR4302547.5712445/1‑94 (MQ=60)
                                                                                   TTGACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGG            <  SRR4302547.3060334/94‑1 (MQ=60)
                                                                                     GACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCT          >  SRR4302547.6166568/1‑94 (MQ=60)
                                                                                     GACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCT          >  SRR4302547.6641852/1‑94 (MQ=60)
                                                                                      ACCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTC         <  SRR4302547.5401818/94‑1 (MQ=60)
                                                                                       CCCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCA        >  SRR4302547.3143328/1‑94 (MQ=60)
                                                                                        CCAGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAT       >  SRR4302547.3608163/1‑94 (MQ=60)
                                                                                          AGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGC     <  SRR4302547.2525555/94‑1 (MQ=60)
                                                                                          AGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGC     >  SRR4302547.3981920/1‑94 (MQ=60)
                                                                                          AGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGC     >  SRR4302547.1691023/1‑94 (MQ=60)
                                                                                          AGGGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGC     >  SRR4302547.5527081/1‑94 (MQ=60)
                                                                                            GGTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCT   <  SRR4302547.1574256/94‑1 (MQ=60)
                                                                                             GTGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTT  >  SRR4302547.1227127/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTT  >  NC_000913/1819973‑1820159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑