Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,414	T→C	Y495H (TAT→CAT)	yeaG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,414	0	T	C	86.7%	619.0 / 77.7	225	Y495H (TAT→CAT)	yeaG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (17/13); new base C (92/103); total (109/116)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 232 total aligned reads displayed.

CAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACC  >  NC_000913/1866322‑1866503

cagAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCt                                                                                           >  2:1252508/1‑93 (MQ=255)

  gAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCAt                                                                                         >  1:3424804/1‑93 (MQ=255)

  aaaCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCAt                                                                                         <  2:2135328/92‑1 (MQ=255)

    acacTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGCCGGCCTACCTTGAATCCCATTc                                                                                       <  1:3743083/90‑1 (MQ=255)

    aCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTc                                                                                       >  2:2732092/1‑93 (MQ=255)

    aCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTc                                                                                       <  2:5922157/93‑1 (MQ=255)

    aCGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTc                                                                                       <  2:5199837/93‑1 (MQ=255)

        tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGaa                                                                                   >  2:5377564/1‑93 (MQ=255)

        tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGaa                                                                                   <  2:6560817/93‑1 (MQ=255)

        tATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGATTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGaa                                                                                   >  1:5067194/1‑93 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  >  1:6219236/1‑93 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  <  1:818280/93‑1 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  >  1:1916679/1‑93 (MQ=255)

         aTCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTGATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAat                                                                                  <  1:1356345/93‑1 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAata                                                                                 >  2:2881637/1‑93 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAata                                                                                 >  1:5991482/1‑93 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAata                                                                                 >  1:2579744/1‑93 (MQ=255)

          tCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAata                                                                                 <  2:3817151/93‑1 (MQ=255)

           cTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAatat                                                                                >  2:647438/1‑93 (MQ=255)

            tGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCCAATATg                                                                               >  1:3862718/1‑93 (MQ=255)

              gAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATggg                                                                             >  1:4900597/1‑93 (MQ=255)

                  tCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaa                                                                         >  2:1130866/1‑93 (MQ=255)

                   ccTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaaa                                                                        >  2:4060470/1‑93 (MQ=255)

                   ccTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaaa                                                                        <  1:4222086/93‑1 (MQ=255)

                   ccTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCaaaa                                                                        <  2:5987098/93‑1 (MQ=255)

                     tGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACa                                                                      <  2:3216138/93‑1 (MQ=255)

                     tGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACa                                                                      >  1:2060516/1‑93 (MQ=255)

                      gAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCACAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAt                                                                     <  2:3048921/93‑1 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    >  1:1059901/1‑93 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    >  2:4195862/1‑93 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    >  2:5069685/1‑93 (MQ=255)

                       aaaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAtt                                                                    <  1:1004466/93‑1 (MQ=255)

                        aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACAttt                                                                   <  2:5464861/93‑1 (MQ=255)

                        aaGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAttt                                                                   >  1:4796711/1‑93 (MQ=255)

                           gTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCg                                                                <  1:4100209/93‑1 (MQ=255)

                             tATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAc                                                              <  2:1918780/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  2:1809883/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  1:6375996/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  1:2666205/93‑1 (MQ=255)

                              aTCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAcc                                                             <  1:3633176/93‑1 (MQ=255)

                               tCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCg                                                            <  2:814840/93‑1 (MQ=255)

                               tCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCg                                                            >  1:6482636/1‑93 (MQ=255)

                                cTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGt                                                           <  2:5758372/93‑1 (MQ=255)

                                 tGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGtt                                                          <  1:1285665/93‑1 (MQ=255)

                                  gATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTa                                                         >  2:805978/1‑93 (MQ=255)

                                  gATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTa                                                         <  1:1832482/93‑1 (MQ=255)

                                  gATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTa                                                         >  1:3555329/1‑93 (MQ=255)

                                   aTCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTAt                                                        >  2:1980392/1‑93 (MQ=255)

                                    tCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATg                                                       <  2:6417142/93‑1 (MQ=255)

                                       cGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTa                                                    <  1:4431259/93‑1 (MQ=255)

                                        gAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAc                                                   >  1:5090559/1‑93 (MQ=255)

                                        gAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTAACTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAc                                                   >  2:4670505/1‑93 (MQ=255)

                                         aaaTATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGGAAAACATTTTCGACCGTTATGTTAcc                                                  >  1:5356964/1‑93 (MQ=255)

                                          aaTATGCCGAGTTTATCGTCAAAGAGATCCAGACGGCCTACCTTGAATCTTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCt                                                 <  1:579622/93‑1 (MQ=255)

                                           atatGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTa                                                <  2:744404/93‑1 (MQ=255)

                                             atGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcg                                              <  1:570014/93‑1 (MQ=255)

                                               gCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgcg                                            <  1:3816656/93‑1 (MQ=255)

                                               gCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgcg                                            <  1:566120/93‑1 (MQ=255)

                                               gCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTAcgcg                                            <  1:288913/93‑1 (MQ=255)

                                                 cGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGa                                          >  1:3380866/1‑93 (MQ=255)

                                                 cGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGa                                          >  2:3562337/1‑93 (MQ=255)

                                                   aGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGAtt                                        >  1:523564/1‑93 (MQ=255)

                                                      ttATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCt                                     >  1:1654795/1‑93 (MQ=255)

                                                      ttAACGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCt                                     <  2:6359773/93‑1 (MQ=255)

                                                         tCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGa                                  <  2:5615279/93‑1 (MQ=255)

                                                           ggCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGAtt                                <  2:2438383/93‑1 (MQ=255)

                                                           ggCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGAtt                                <  2:3494322/93‑1 (MQ=255)

                                                             cAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtca                              <  1:2083587/93‑1 (MQ=255)

                                                              aaaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcag                             <  1:4000061/93‑1 (MQ=255)

                                                              aaaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcag                             >  2:3078524/1‑93 (MQ=255)

                                                               aaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcagg                            <  1:6091231/93‑1 (MQ=255)

                                                               aaGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcagg                            >  2:449597/1‑93 (MQ=255)

                                                                agagATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcagga                           <  2:3360023/93‑1 (MQ=255)

                                                                 gagaTCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggat                          >  1:3648147/1‑93 (MQ=255)

                                                                 gagaTCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggat                          >  1:3076559/1‑93 (MQ=255)

                                                                    aTCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcag                       >  2:4036579/1‑93 (MQ=255)

                                                                     tCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcagg                      >  2:1575540/1‑93 (MQ=255)

                                                                      ccAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcagga                     >  1:4495618/1‑93 (MQ=255)

                                                                      ccAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATtcaggatcagga                     >  1:4707010/1‑93 (MQ=255)

                                                                         gACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTa                  <  2:6206782/93‑1 (MQ=255)

                                                                          aCGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTAt                 <  2:2756252/93‑1 (MQ=255)

                                                                           cGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATc                >  2:4260865/1‑93 (MQ=255)

                                                                             gCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATcgc              >  1:1238987/1‑93 (MQ=255)

                                                                             gCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATcgc              >  1:2295062/1‑93 (MQ=255)

                                                                               cTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGa            <  1:2128348/93‑1 (MQ=255)

                                                                               cTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGa            >  1:3945013/1‑93 (MQ=255)

                                                                                tACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGAt           >  1:5851595/1‑93 (MQ=255)

                                                                                tACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGAt           <  2:1687807/93‑1 (MQ=255)

                                                                                  ccTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATcc         >  1:305537/1‑93 (MQ=255)

                                                                                  ccTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATcc         <  1:4861011/93‑1 (MQ=255)

                                                                                  ccTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATcc         >  2:4508927/1‑93 (MQ=255)

                                                                                   cTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        >  1:6609812/1‑93 (MQ=255)

                                                                                   cTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        <  1:5487403/93‑1 (MQ=255)

                                                                                   cTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        >  2:6634189/1‑93 (MQ=255)

                                                                                   cTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCg        <  1:962354/93‑1 (MQ=255)

                                                                                     tGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGa      <  2:3124191/93‑1 (MQ=255)

                                                                                      gAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGAt     >  1:4312004/1‑93 (MQ=255)

                                                                                       aaTCCCATTCCGAATATGGGCAAAACGTTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCCCGATCCGGATa    <  1:240184/93‑1 (MQ=255)

                                                                                         tCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATAcc  <  2:4838731/93‑1 (MQ=255)

                                                                                         tCCCATACCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATAcc  <  1:2534992/93‑1 (MQ=255)

CAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACC  >  NC_000913/1866322‑1866503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGC  >  NC_000913/1866321‑1866507

GCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCC                                                                                               <  SRR4302547.3525612/94‑1 (MQ=60)

 CAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCA                                                                                              >  SRR4302547.2686767/1‑94 (MQ=60)

  atacCGCTATCTGGAGTTCCTGAAGGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAACGAGATCCAGACGGCCTCCCTTGAATCCTAT                                                                                             <  SRR4302547.1234661/90‑1 (MQ=60)

  AGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCAT                                                                                             >  SRR4302547.3494545/1‑94 (MQ=60)

  AGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGAGCCAGACGGCCTACCTTGAATTCTAT                                                                                             >  SRR4302547.5082795/1‑94 (MQ=60)

    AACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTC                                                                                           <  SRR4302547.4819078/94‑1 (MQ=60)

     ACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCC                                                                                          <  SRR4302547.6090143/94‑1 (MQ=60)

     ACACTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGCCGGCCTACCTTGAATCCCATTCC                                                                                          <  SRR4302547.3819492/94‑1 (MQ=60)

       GCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGA                                                                                        >  SRR4302547.6474959/1‑94 (MQ=60)

       GCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGA                                                                                        >  SRR4302547.3609821/1‑94 (MQ=60)

        CTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAA                                                                                       <  SRR4302547.4137933/94‑1 (MQ=60)

        CTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGATTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAA                                                                                       >  SRR4302547.5171745/1‑94 (MQ=60)

         TATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAAT                                                                                      >  SRR4302547.6348391/1‑94 (MQ=60)

         TATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAAT                                                                                      >  SRR4302547.608178/1‑94 (MQ=60)

         TATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAAT                                                                                      >  SRR4302547.3024882/1‑94 (MQ=60)

         TATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAAT                                                                                      >  SRR4302547.1954630/1‑94 (MQ=60)

          ATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATA                                                                                     <  SRR4302547.2794644/94‑1 (MQ=60)

          ATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATA                                                                                     <  SRR4302547.833841/94‑1 (MQ=60)

          ATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATA                                                                                     >  SRR4302547.6115791/1‑94 (MQ=60)

          ATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATA                                                                                     >  SRR4302547.2631807/1‑94 (MQ=60)

          ATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTGATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATA                                                                                     <  SRR4302547.1383562/94‑1 (MQ=60)

          ATCTGGAGTACCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATA                                                                                     >  SRR4302547.6626340/1‑94 (MQ=60)

            CTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATG                                                                                   >  SRR4302547.4229938/1‑94 (MQ=60)

            CTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCCAATATG                                                                                   >  SRR4302547.3941317/1‑94 (MQ=60)

              GGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGG                                                                                 >  SRR4302547.5002074/1‑94 (MQ=60)

               GAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGC                                                                                <  SRR4302547.5174569/94‑1 (MQ=60)

                AGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCA                                                                               >  SRR4302547.5350201/1‑94 (MQ=60)

                AGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCA                                                                               >  SRR4302547.3636016/1‑94 (MQ=60)

                  TTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAA                                                                             <  SRR4302547.2539526/94‑1 (MQ=60)

                   TCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAA                                                                            >  SRR4302547.4421834/1‑94 (MQ=60)

                   TCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAA                                                                            >  SRR4302547.5634290/1‑94 (MQ=60)

                    CCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAAC                                                                           <  SRR4302547.4308320/94‑1 (MQ=60)

                     CTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACA                                                                          >  SRR4302547.2101631/1‑94 (MQ=60)

                      TGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACAT                                                                         <  SRR4302547.2390318/94‑1 (MQ=60)

                       GAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATT                                                                        >  SRR4302547.1080982/1‑94 (MQ=60)

                        AAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTT                                                                       >  SRR4302547.4896104/1‑94 (MQ=60)

                        AAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTT                                                                       <  SRR4302547.1024059/94‑1 (MQ=60)

                        AAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTT                                                                       >  SRR4302547.892082/1‑94 (MQ=60)

                         AAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTT                                                                      <  SRR4302547.4989144/94‑1 (MQ=60)

                         AAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTT                                                                      <  SRR4302547.1845900/94‑1 (MQ=60)

                           GGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCG                                                                    <  SRR4302547.1323202/94‑1 (MQ=60)

                            GTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGA                                                                   <  SRR4302547.4183591/94‑1 (MQ=60)

                             TTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAC                                                                  >  SRR4302547.5418636/1‑94 (MQ=60)

                             TTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGAC                                                                  <  SRR4302547.3199016/94‑1 (MQ=60)

                              TATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACC                                                                 >  SRR4302547.5930755/1‑94 (MQ=60)

                               ATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCG                                                                <  SRR4302547.2719779/94‑1 (MQ=60)

                               ATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCG                                                                <  SRR4302547.3707287/94‑1 (MQ=60)

                               ATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCG                                                                <  SRR4302547.6509040/94‑1 (MQ=60)

                               ATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCG                                                                >  SRR4302547.6617595/1‑94 (MQ=60)

                                  tgctgCCGAAATATTCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTA                                                             <  SRR4302547.2007500/89‑1 (MQ=60)

                                  TGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTA                                                             <  SRR4302547.1311560/94‑1 (MQ=60)

                                  TGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTA                                                             >  SRR4302547.3627882/1‑94 (MQ=60)

                                   GATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTAT                                                            <  SRR4302547.5255327/94‑1 (MQ=60)

                                   GATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTAT                                                            <  SRR4302547.1868705/94‑1 (MQ=60)

                                    ATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATG                                                           <  SRR4302547.38475/94‑1 (MQ=60)

                                      CCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTT                                                         <  SRR4302547.2636007/94‑1 (MQ=60)

                                      CCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTT                                                         >  SRR4302547.1313541/1‑94 (MQ=60)

                                       CCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTA                                                        >  SRR4302547.5103418/1‑94 (MQ=60)

                                        CGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAC                                                       >  SRR4302547.5195562/1‑94 (MQ=60)

                                        CGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAC                                                       >  SRR4302547.4167235/1‑94 (MQ=60)

                                        CGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAC                                                       >  SRR4302547.6190899/1‑94 (MQ=60)

                                        CGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAC                                                       <  SRR4302547.4522853/94‑1 (MQ=60)

                                        CGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTAC                                                       >  SRR4302547.312061/1‑94 (MQ=60)

                                         GAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGGAAAACATTTTCGACCGTTATGTTACC                                                      >  SRR4302547.5467458/1‑94 (MQ=60)

                                         GAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACC                                                      >  SRR4302547.6375187/1‑94 (MQ=60)

                                          AAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCT                                                     <  SRR4302547.3947049/94‑1 (MQ=60)

                                           AATATGCCGAGTTTATCGTCAAAGAGATCCAGACGGCCTACCTTGAATCTTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTA                                                    <  SRR4302547.590016/94‑1 (MQ=60)

                                              ATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGC                                                 <  SRR4302547.580261/94‑1 (MQ=60)

                                               TGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCG                                                <  SRR4302547.5030526/94‑1 (MQ=60)

                                               TGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCG                                                <  SRR4302547.5811395/94‑1 (MQ=60)

                                                GCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGG                                               <  SRR4302547.3894424/94‑1 (MQ=60)

                                                GCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGG                                               <  SRR4302547.2170052/94‑1 (MQ=60)

                                                GCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGG                                               <  SRR4302547.576301/94‑1 (MQ=60)

                                                GCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGG                                               <  SRR4302547.294277/94‑1 (MQ=60)

                                                 CCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGA                                              >  SRR4302547.6334392/1‑94 (MQ=60)

                                                 CCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGA                                              >  SRR4302547.3449574/1‑94 (MQ=60)

                                                  CGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGAT                                             >  SRR4302547.6535660/1‑94 (MQ=60)

                                                   GAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATT                                            >  SRR4302547.533042/1‑94 (MQ=60)

                                                   GAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATT                                            >  SRR4302547.4941048/1‑94 (MQ=60)

                                                    AGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTT                                           <  SRR4302547.2110955/94‑1 (MQ=60)

                                                     GTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTC                                          >  SRR4302547.4411010/1‑94 (MQ=60)

                                                     GTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTC                                          <  SRR4302547.1077615/94‑1 (MQ=60)

                                                      TTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCT                                         <  SRR4302547.5542409/94‑1 (MQ=60)

                                                      TTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCT                                         <  SRR4302547.2733335/94‑1 (MQ=60)

                                                      TTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCT                                         <  SRR4302547.3641902/94‑1 (MQ=60)

                                                      TTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCT                                         >  SRR4302547.1687444/1‑94 (MQ=60)

                                                        tacctGCAAAGACATCCAGACGTCCTACCTTGATTCCCCTTCCGAATATGGCCAAACCATTTTCGACCGTTATGTTGCCTACGCGGATTTCTGG                                       <  SRR4302547.3240808/89‑1 (MQ=55)

                                                             GCAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCA                                  <  SRR4302547.3749273/94‑1 (MQ=60)

                                                              CAAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAG                                 <  SRR4302547.2125134/94‑1 (MQ=60)

                                                               AAAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGG                                <  SRR4302547.4081372/94‑1 (MQ=60)

                                                                AAGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGA                               <  SRR4302547.6217589/94‑1 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGAT                              <  SRR4302547.4342785/94‑1 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTATCTACGCGGATTTCTGGATTCAGGAT                              >  SRR4302547.2054812/1‑94 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGAT                              <  SRR4302547.1435528/94‑1 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGAT                              <  SRR4302547.2332314/94‑1 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGAT                              >  SRR4302547.3137668/1‑94 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGAT                              <  SRR4302547.344554/94‑1 (MQ=60)

                                                                 AGAGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGAT                              >  SRR4302547.3722556/1‑94 (MQ=60)

                                                                   AGATCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCA                            <  SRR4302547.2631136/94‑1 (MQ=60)

                                                                      TCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGA                         >  SRR4302547.3931584/1‑94 (MQ=60)

                                                                      TCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGA                         >  SRR4302547.4804489/1‑94 (MQ=60)

                                                                      TCCAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGA                         >  SRR4302547.4588470/1‑94 (MQ=60)

                                                                       CCAGACGGCCTACCTCGACACCCCTTCCGAATATGGGCAAAACATTTAAGTCCGTTATATTGCCTACGCGGATTTCTGGATTCAGGATCAGGAG                        <  SRR4302547.3303880/94‑1 (MQ=44)

                                                                        CAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGT                       <  SRR4302547.992931/94‑1 (MQ=60)

                                                                        CAGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGT                       <  SRR4302547.3839295/94‑1 (MQ=60)

                                                                         AGACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTA                      >  SRR4302547.801608/1‑94 (MQ=60)

                                                                           ACGTCCTACCTTGAAGCCCATTCCGATTATGGGCAAAACATTTTCGACCGTTATGTTACCTGCGCGGATTTCTGGATTCAGGATCAGGAGTATC                    <  SRR4302547.1935680/94‑1 (MQ=60)

                                                                           ACGGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATC                    <  SRR4302547.2151142/94‑1 (MQ=60)

                                                                             GGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGC                  >  SRR4302547.1263998/1‑94 (MQ=60)

                                                                             GGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGC                  >  SRR4302547.2341570/1‑94 (MQ=60)

                                                                             GGCCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGC                  <  SRR4302547.5815026/94‑1 (MQ=60)

                                                                               CCTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGA                >  SRR4302547.4025251/1‑94 (MQ=60)

                                                                                CTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGAT               <  SRR4302547.2170760/94‑1 (MQ=60)

                                                                                CTACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGAT               >  SRR4302547.5972636/1‑94 (MQ=60)

                                                                                 TACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATC              >  SRR4302547.4836159/1‑94 (MQ=60)

                                                                                 TACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATC              <  SRR4302547.4060222/94‑1 (MQ=60)

                                                                                 TACCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATC              <  SRR4302547.3721806/94‑1 (MQ=60)

                                                                                  ACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCC             >  SRR4302547.311223/1‑94 (MQ=60)

                                                                                   CCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCG            >  SRR4302547.6747400/1‑94 (MQ=60)

                                                                                   CCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCG            <  SRR4302547.4961744/94‑1 (MQ=60)

                                                                                   CCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCG            <  SRR4302547.4528159/94‑1 (MQ=60)

                                                                                   CCTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCG            <  SRR4302547.2460432/94‑1 (MQ=60)

                                                                                    CTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGG           <  SRR4302547.5600718/94‑1 (MQ=60)

                                                                                    CTTGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGG           <  SRR4302547.981046/94‑1 (MQ=60)

                                                                                     TTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGA          >  SRR4302547.192554/1‑94 (MQ=60)

                                                                                      TGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGAT         >  SRR4302547.4400723/1‑94 (MQ=60)

                                                                                      TGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGAT         <  SRR4302547.5860211/94‑1 (MQ=60)

                                                                                      TGAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGCAT         >  SRR4302547.4714356/1‑94 (MQ=60)

                                                                                       GAATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATA        >  SRR4302547.942134/1‑94 (MQ=60)

                                                                                        AATCCCATTCCGAATATGGGCAAAACGTTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCCCGATCCGGATAC       <  SRR4302547.244680/94‑1 (MQ=60)

                                                                                         ATCCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACC      >  SRR4302547.1273300/1‑94 (MQ=60)

                                                                                          TCCCATACCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCG     <  SRR4302547.2586221/94‑1 (MQ=60)

                                                                                           CCCATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGG    >  SRR4302547.2511022/1‑94 (MQ=60)

                                                                                             TATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGC  >  SRR4302547.5216600/1‑94 (MQ=60)

                                                                                             CATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGC  >  SRR4302547.3231706/1‑94 (MQ=60)

                                                                                             CATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGC  <  SRR4302547.5999715/94‑1 (MQ=60)

GCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGC  >  NC_000913/1866321‑1866507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`