Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,913,489	A→G	C24R (TGT→CGT)	proQ ←	ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,913,489	0	A	G	82.5%	543.3 / 114.1	234	C24R (TGT→CGT)	proQ	ProQ
Reads supporting (aligned to +/- strand): ref base A (23/18); new base G (89/104); total (112/122)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

Only 100 of 241 total aligned reads displayed.

TTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGA  >  NC_000913/1913397‑1913580

tttGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACa                                                                                             <  2:3388719/93‑1 (MQ=255)

   gCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTg                                                                                          <  1:5829063/93‑1 (MQ=255)

    cTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTgg                                                                                         >  1:5562359/1‑93 (MQ=255)

     tCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTggg                                                                                        >  1:2015050/1‑93 (MQ=255)

     tCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTggg                                                                                        >  1:227143/1‑93 (MQ=255)

       aGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGa                                                                                      >  2:6127733/1‑93 (MQ=255)

        ggTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGaa                                                                                     <  2:5739314/93‑1 (MQ=255)

        ggTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGaa                                                                                     <  2:81175/93‑1 (MQ=255)

           tCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAc                                                                                  >  2:3286966/1‑93 (MQ=255)

           tCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAc                                                                                  <  1:4985414/93‑1 (MQ=255)

           tCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAc                                                                                  >  1:810043/1‑93 (MQ=255)

             aTTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGt                                                                                >  2:5223172/1‑93 (MQ=255)

             aTTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGt                                                                                <  2:6596373/93‑1 (MQ=255)

             aTTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGt                                                                                >  1:4042707/1‑93 (MQ=255)

              tttCCCCAGCAACACGATCGACTAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGtt                                                                               <  2:6447770/93‑1 (MQ=255)

              tttCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGtt                                                                               <  2:5082401/93‑1 (MQ=255)

               ttCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTc                                                                              <  2:1041362/93‑1 (MQ=255)

               ttCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTc                                                                              <  2:2861825/93‑1 (MQ=255)

                tCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCg                                                                             <  2:215729/93‑1 (MQ=255)

                tCCCAAGCAACACGATCGACCAAATCCTGAAAAATCCCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCg                                                                             <  1:186672/93‑1 (MQ=255)

                  cccAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGc                                                                           <  1:442936/93‑1 (MQ=255)

                  cccAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGc                                                                           >  1:1525955/1‑93 (MQ=255)

                   ccAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCgggc                                                                          >  2:1317292/1‑91 (MQ=255)

                   ccAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGcc                                                                          >  2:1705512/1‑93 (MQ=255)

                    cAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCa                                                                         <  1:345369/93‑1 (MQ=255)

                     aGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAg                                                                        >  2:4909097/1‑93 (MQ=255)

                     aGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAg                                                                        <  2:3030411/93‑1 (MQ=255)

                      gCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGa                                                                       >  1:4920220/1‑93 (MQ=255)

                        aaCACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGaaa                                                                     <  1:2087005/93‑1 (MQ=255)

                        aaCACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGaaa                                                                     <  2:2613269/93‑1 (MQ=255)

                         acacGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGTACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAc                                                                    <  1:3027926/93‑1 (MQ=255)

                         acacGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAc                                                                    <  1:4048522/93‑1 (MQ=255)

                         acacGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAc                                                                    <  2:5995196/93‑1 (MQ=255)

                         acacGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAc                                                                    <  2:3346549/93‑1 (MQ=255)

                         acacGATCGACCAAATCCTGAAAAATACCGAATTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGCAAACGTTCGGCCAGAAAc                                                                    >  1:5307344/1‑93 (MQ=255)

                          cacGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAcg                                                                   <  2:6062875/93‑1 (MQ=255)

                          cacGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAcg                                                                   <  1:566724/93‑1 (MQ=255)

                           acGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAAcgc                                                                  <  2:4856854/93‑1 (MQ=255)

                            cGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAAcgcg                                                                 >  1:4580108/1‑93 (MQ=255)

                              aTCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGAt                                                               <  1:6318394/93‑1 (MQ=255)

                               tCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGAtt                                                              <  2:4835656/93‑1 (MQ=255)

                               ccGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGAtt                                                              <  2:5013831/92‑1 (MQ=255)

                                 gtccAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTat                                                            >  1:5275198/3‑92 (MQ=255)

                                 gACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTAc                                                            <  1:4850826/93‑1 (MQ=255)

                                 gACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTAc                                                            >  2:5915969/1‑93 (MQ=255)

                                  aCCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTAct                                                           >  2:4367344/1‑93 (MQ=255)

                                  aCCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTAct                                                           >  2:4234518/1‑93 (MQ=255)

                                   ccAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTActt                                                          <  1:6586100/93‑1 (MQ=255)

                                   ccAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTActt                                                          >  1:4222197/1‑93 (MQ=255)

                                    cAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtc                                                         <  1:320283/93‑1 (MQ=255)

                                     aaaTCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtct                                                        <  1:6255971/93‑1 (MQ=255)

                                       aTCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCttt                                                      >  1:1931520/1‑93 (MQ=255)

                                         ccTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTAc                                                    <  1:1185353/93‑1 (MQ=255)

                                          cTGAAAAATACCGATTTTCAGCGGACGCGCTTCCCCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACt                                                   <  2:3910225/93‑1 (MQ=255)

                                          cTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACt                                                   <  1:3748199/93‑1 (MQ=255)

                                           tGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTg                                                  <  1:1333951/93‑1 (MQ=255)

                                            gAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGc                                                 <  2:5492331/93‑1 (MQ=255)

                                               aaaTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTAt                                              <  1:1015700/93‑1 (MQ=255)

                                                aaTACCGATTTTCAGCGGACGCACTTCTCCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTAtt                                             <  2:2678282/93‑1 (MQ=255)

                                                 aTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTAttt                                            >  1:3492311/1‑92 (MQ=255)

                                                 aTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTc                                            >  2:1927044/1‑93 (MQ=255)

                                                  tACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTAttta                                           >  1:6268975/1‑91 (MQ=255)

                                                  tACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCa                                           >  2:485348/1‑93 (MQ=255)

                                                  tACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCa                                           <  2:4595052/93‑1 (MQ=255)

                                                    ccGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAAc                                         <  2:3140432/93‑1 (MQ=255)

                                                    ccGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAAc                                         >  2:1746972/1‑93 (MQ=255)

                                                      gATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACtt                                       >  1:168266/1‑93 (MQ=255)

                                                       aTTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTa                                      >  1:3269458/1‑93 (MQ=255)

                                                        ttttCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAg                                     <  1:4355440/93‑1 (MQ=255)

                                                         tttCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAgg                                    <  2:4175859/93‑1 (MQ=255)

                                                          ttCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGt                                   <  2:2034677/93‑1 (MQ=255)

                                                            cAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTg                                 >  1:4711562/1‑93 (MQ=255)

                                                             aGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGa                                <  2:1079883/93‑1 (MQ=255)

                                                              gCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAt                               >  2:2944754/1‑93 (MQ=255)

                                                               cGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAtt                              <  1:3790548/93‑1 (MQ=255)

                                                               cGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAtt                              >  1:396022/1‑93 (MQ=255)

                                                                ggACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAttt                             >  2:861901/1‑93 (MQ=255)

                                                                 gACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAtttt                            >  2:372957/1‑93 (MQ=255)

                                                                 gACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAtttt                            >  1:2442891/1‑93 (MQ=255)

                                                                  aCGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTc                           <  1:2975941/93‑1 (MQ=255)

                                                                   cgcgCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTcc                          >  2:5313983/1‑93 (MQ=255)

                                                                    gcgcTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCa                         >  1:5111349/1‑93 (MQ=255)

                                                                    gcgcTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCa                         <  2:634410/93‑1 (MQ=255)

                                                                       cTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGa                      >  1:5459527/1‑93 (MQ=255)

                                                                        ttCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGaa                     >  1:5966870/1‑93 (MQ=255)

                                                                           aCCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAAtt                  <  1:1379654/93‑1 (MQ=255)

                                                                            ccTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAAttt                 >  2:4078746/1‑93 (MQ=255)

                                                                               tCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCt              >  2:2309710/1‑93 (MQ=255)

                                                                               tCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCt              <  2:3083395/93‑1 (MQ=255)

                                                                                 cGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTCCTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGa            <  1:1757351/93‑1 (MQ=255)

                                                                                 cGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGa            <  2:2278927/93‑1 (MQ=255)

                                                                                 cGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGa            <  1:273109/93‑1 (MQ=255)

                                                                                  gCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGAt           <  1:2588924/93‑1 (MQ=255)

                                                                                     cTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTAc        <  2:4932147/93‑1 (MQ=255)

                                                                                      tGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACa       <  2:901165/93‑1 (MQ=255)

                                                                                        aaaCGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAAc     >  2:6533684/1‑93 (MQ=255)

                                                                                        aaaCGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAAc     <  1:1351864/93‑1 (MQ=255)

                                                                                        aaaCGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAAc     <  1:5881324/93‑1 (MQ=255)

                                                                                        aaaCAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAAc     <  2:5650802/93‑1 (MQ=255)

                                                                                          aCAGTGCGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACgg   <  2:5350685/93‑1 (MQ=255)

TTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGA  >  NC_000913/1913397‑1913580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACG  >  NC_000913/1913396‑1913582

TTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACG                                                                                               <  SRR4302547.1118390/94‑1 (MQ=60)

TTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACG                                                                                               <  SRR4302547.6239200/94‑1 (MQ=60)

 TTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGG                                                                                              <  SRR4302547.2539994/94‑1 (MQ=60)

 TTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTCCCGCACTGAAACGG                                                                                              <  SRR4302547.4146615/94‑1 (MQ=60)

  TTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGT                                                                                             <  SRR4302547.3134493/94‑1 (MQ=60)

  TTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGT                                                                                             <  SRR4302547.4208456/94‑1 (MQ=60)

   TGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTG                                                                                            <  SRR4302547.4936404/94‑1 (MQ=60)

    GCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGG                                                                                           <  SRR4302547.5949689/94‑1 (MQ=60)

    GCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGG                                                                                           >  SRR4302547.5677082/1‑94 (MQ=60)

     CTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGG                                                                                          <  SRR4302547.3739788/94‑1 (MQ=60)

     CTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGG                                                                                          >  SRR4302547.231407/1‑94 (MQ=60)

     CTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGG                                                                                          >  SRR4302547.2055139/1‑94 (MQ=60)

      TCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAAAACCGATTTTCAGCGGCGGGGCTTCACCTTCCGCACTGAAACGGTGGGA                                                                                         >  SRR4302547.1954968/1‑94 (MQ=60)

         GGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAA                                                                                      <  SRR4302547.863261/94‑1 (MQ=60)

          GTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAA                                                                                     >  SRR4302547.4704188/1‑94 (MQ=60)

          GTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAA                                                                                     >  SRR4302547.6410878/1‑94 (MQ=60)

          GTTAATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAA                                                                                     <  SRR4302547.2270157/94‑1 (MQ=60)

           TTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAC                                                                                    <  SRR4302547.5974813/94‑1 (MQ=60)

           TTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAC                                                                                    <  SRR4302547.4581577/94‑1 (MQ=60)

           TTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAC                                                                                    <  SRR4302547.3501527/94‑1 (MQ=60)

           TTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAAC                                                                                    >  SRR4302547.825392/1‑94 (MQ=60)

           TTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAAC                                                                                    >  SRR4302547.6329156/1‑94 (MQ=60)

            TCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACG                                                                                   <  SRR4302547.5088498/94‑1 (MQ=60)

             CATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGT                                                                                  >  SRR4302547.4124801/1‑94 (MQ=60)

             CATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGT                                                                                  >  SRR4302547.4124802/1‑94 (MQ=60)

                 TCCCAAGCAACACGATCGACCAAATCCTGAAAAATCCCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGG                                                                              <  SRR4302547.190187/94‑1 (MQ=60)

                  CCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGC                                                                             >  SRR4302547.1556325/1‑94 (MQ=60)

                   CCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCC                                                                            <  SRR4302547.451186/94‑1 (MQ=60)

                   CCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCC                                                                            <  SRR4302547.5270197/94‑1 (MQ=60)

                    CCAGCAACACGATCGACCAAATCCTGAAAAATACCTATTGTCATCGGTCGCGCTTCCCCTTCCGCACTGAAACCGTAGGAAAAACGTTCGTCCA                                                                           <  SRR4302547.6634177/94‑1 (MQ=55)

                     CAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAG                                                                          <  SRR4302547.351782/94‑1 (MQ=60)

                     CAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAG                                                                          >  SRR4302547.3285811/1‑94 (MQ=60)

                      AGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGA                                                                         >  SRR4302547.5022122/1‑94 (MQ=60)

                        CAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAC                                                                       >  SRR4302547.5951617/1‑94 (MQ=60)

                         AACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAC                                                                      <  SRR4302547.5006486/94‑1 (MQ=60)

                         AACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAAC                                                                      <  SRR4302547.2128626/94‑1 (MQ=60)

                         AACACGATCGACCAAATCCTGAAAAATACCGAATTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGCAAACGTTCGGCCAGAAAC                                                                      >  SRR4302547.5416809/1‑94 (MQ=60)

                          ACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGTACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACG                                                                     <  SRR4302547.3088134/94‑1 (MQ=60)

                          ACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACG                                                                     <  SRR4302547.4130740/94‑1 (MQ=60)

                          ACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACG                                                                     <  SRR4302547.4856296/94‑1 (MQ=60)

                           CACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGC                                                                    >  SRR4302547.5106746/1‑94 (MQ=60)

                           CACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGC                                                                    <  SRR4302547.576909/94‑1 (MQ=60)

                            ACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCG                                                                   >  SRR4302547.5412558/1‑94 (MQ=60)

                            ACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCG                                                                   >  SRR4302547.4674901/1‑94 (MQ=60)

                            ACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCG                                                                   >  SRR4302547.6396884/1‑94 (MQ=60)

                              GATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGAT                                                                 >  SRR4302547.6224912/1‑94 (MQ=60)

                               ATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATT                                                                <  SRR4302547.6449776/94‑1 (MQ=60)

                                 CGTCCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTAT                                                              >  SRR4302547.5383939/1‑94 (MQ=60)

                                  GACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACT                                                             <  SRR4302547.4951343/94‑1 (MQ=60)

                                   ACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTT                                                            >  SRR4302547.400647/1‑94 (MQ=60)

                                   ACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTT                                                            >  SRR4302547.4308432/1‑94 (MQ=60)

                                    CCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTC                                                           <  SRR4302547.6723227/94‑1 (MQ=60)

                                     CAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCT                                                          <  SRR4302547.326240/94‑1 (MQ=60)

                                      AAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTT                                                         <  SRR4302547.6385912/94‑1 (MQ=60)

                                       AATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTT                                                        >  SRR4302547.2863480/1‑94 (MQ=60)

                                       AATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTT                                                        >  SRR4302547.1969812/1‑94 (MQ=60)

                                         TCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTAC                                                      >  SRR4302547.3189074/1‑94 (MQ=60)

                                         TCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTAC                                                      <  SRR4302547.185007/94‑1 (MQ=60)

                                          CCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACT                                                     <  SRR4302547.1209293/94‑1 (MQ=60)

                                           CTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTG                                                    <  SRR4302547.3824712/94‑1 (MQ=60)

                                            TGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGC                                                   >  SRR4302547.4460296/1‑94 (MQ=60)

                                            TGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGC                                                   <  SRR4302547.1360765/94‑1 (MQ=60)

                                            TGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGC                                                   >  SRR4302547.788325/1‑94 (MQ=60)

                                             GAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCT                                                  >  SRR4302547.3854213/1‑94 (MQ=60)

                                              AAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTA                                                 <  SRR4302547.1224337/94‑1 (MQ=60)

                                                AAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATT                                               >  SRR4302547.2773783/1‑94 (MQ=60)

                                                AAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATT                                               <  SRR4302547.1035588/94‑1 (MQ=60)

                                                 AATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTT                                              >  SRR4302547.3563580/1‑94 (MQ=60)

                                                  ATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTTA                                             >  SRR4302547.6399203/1‑94 (MQ=60)

                                                  ATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCA                                             <  SRR4302547.3086243/94‑1 (MQ=60)

                                                    ACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAAC                                           <  SRR4302547.1377051/94‑1 (MQ=60)

                                                     CCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACT                                          <  SRR4302547.1523317/94‑1 (MQ=60)

                                                      CGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTT                                         >  SRR4302547.1859979/1‑94 (MQ=60)

                                                      CGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTT                                         >  SRR4302547.1361366/1‑94 (MQ=60)

                                                      CGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTT                                         >  SRR4302547.171352/1‑94 (MQ=60)

                                                       GATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTA                                        >  SRR4302547.3334896/1‑94 (MQ=60)

                                                        ATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAG                                       <  SRR4302547.4326863/94‑1 (MQ=60)

                                                        ATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAG                                       <  SRR4302547.1579853/94‑1 (MQ=60)

                                                         TTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGG                                      <  SRR4302547.2030400/94‑1 (MQ=60)

                                                         TTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGG                                      <  SRR4302547.4444910/94‑1 (MQ=60)

                                                            TCAGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTG                                   >  SRR4302547.4809120/1‑94 (MQ=60)

                                                              AGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAT                                 >  SRR4302547.651816/1‑94 (MQ=60)

                                                              AGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAT                                 <  SRR4302547.2633047/94‑1 (MQ=60)

                                                              AGCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAT                                 <  SRR4302547.704760/94‑1 (MQ=60)

                                                               GCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCTGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATT                                >  SRR4302547.6693753/1‑94 (MQ=60)

                                                               GCGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATT                                >  SRR4302547.403381/1‑94 (MQ=60)

                                                               GCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATT                                >  SRR4302547.6067373/1‑94 (MQ=60)

                                                                CGGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTT                               <  SRR4302547.3867838/94‑1 (MQ=60)

                                                                 GGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTT                              >  SRR4302547.2492301/1‑94 (MQ=60)

                                                                 GGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTT                              >  SRR4302547.241260/1‑94 (MQ=60)

                                                                 GGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTT                              >  SRR4302547.3442114/1‑94 (MQ=60)

                                                                 GGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTT                              >  SRR4302547.4548400/1‑94 (MQ=60)

                                                                 GGACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACCTAGGTTGATTTT                              >  SRR4302547.4086989/1‑94 (MQ=60)

                                                                   ACGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCC                            <  SRR4302547.3035156/94‑1 (MQ=60)

                                                                    CGCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCA                           >  SRR4302547.5216680/1‑94 (MQ=60)

                                                                    CGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCA                           >  SRR4302547.5472477/1‑94 (MQ=60)

                                                                     GCGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCAT                          <  SRR4302547.5659692/94‑1 (MQ=60)

                                                                      CGCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATG                         <  SRR4302547.4189724/94‑1 (MQ=60)

                                                                       GCTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGA                        >  SRR4302547.5572326/1‑94 (MQ=60)

                                                                        CTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAA                       >  SRR4302547.6090630/1‑94 (MQ=60)

                                                                        CTTCACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAA                       <  SRR4302547.2038006/94‑1 (MQ=60)

                                                                           CACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATT                    >  SRR4302547.107050/1‑94 (MQ=60)

                                                                           CACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATT                    >  SRR4302547.208909/1‑94 (MQ=60)

                                                                           CACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATT                    <  SRR4302547.5745287/94‑1 (MQ=60)

                                                                           CACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATT                    >  SRR4302547.538503/1‑94 (MQ=60)

                                                                            ACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTT                   <  SRR4302547.1407311/94‑1 (MQ=60)

                                                                            ACCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTT                   >  SRR4302547.3597986/1‑94 (MQ=60)

                                                                             CCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTC                  <  SRR4302547.1727047/94‑1 (MQ=60)

                                                                             CCTTCCGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTC                  <  SRR4302547.2379970/94‑1 (MQ=60)

                                                                                  CGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTCCTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGAT             <  SRR4302547.1792151/94‑1 (MQ=60)

                                                                                  CGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGAT             <  SRR4302547.278215/94‑1 (MQ=60)

                                                                                  CGCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGAT             <  SRR4302547.5072903/94‑1 (MQ=60)

                                                                                   GCACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATT            <  SRR4302547.2641152/94‑1 (MQ=60)

                                                                                   GCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATT            >  SRR4302547.1388841/1‑94 (MQ=60)

                                                                                    CACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTA           <  SRR4302547.14111/94‑1 (MQ=60)

                                                                                    CACTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTA           <  SRR4302547.5852061/94‑1 (MQ=60)

                                                                                      CTGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACA         >  SRR4302547.4109747/1‑94 (MQ=60)

                                                                                      CTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACA         >  SRR4302547.4140237/1‑94 (MQ=60)

                                                                                      CTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACA         >  SRR4302547.3240786/1‑94 (MQ=60)

                                                                                       TGAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAA        <  SRR4302547.5174366/94‑1 (MQ=60)

                                                                                        GAAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAAC       <  SRR4302547.3528941/94‑1 (MQ=60)

                                                                                         AAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACG      <  SRR4302547.1379016/94‑1 (MQ=60)

                                                                                         AAACGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACG      <  SRR4302547.6003093/94‑1 (MQ=60)

                                                                                           ACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGA    >  SRR4302547.2741113/1‑94 (MQ=60)

                                                                                            CGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGAC   >  SRR4302547.925437/1‑94 (MQ=60)

                                                                                            CGGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGAC   >  SRR4302547.5703867/1‑94 (MQ=60)

                                                                                            CAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGAC   <  SRR4302547.5749387/94‑1 (MQ=60)

                                                                                             GGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACG  <  SRR4302547.2376436/94‑1 (MQ=60)

TTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACG  >  NC_000913/1913396‑1913582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`