Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,019,024 T→C Y306H (TAT→CAT)  fliM → FliM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,0240TC86.8% 664.2 / 81.6 242Y306H (TAT→CAT) fliMFliM
Reads supporting (aligned to +/- strand):  ref base T (13/19);  new base C (108/102);  total (121/121)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 265 total aligned reads displayed.

TTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGT  >  NC_000913/2018933‑2019115
                                                                                           |                                                                                           
   aaaaCTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATgg                                                                                         >  2:1496986/1‑93 (MQ=255)
      aCTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCAc                                                                                      <  1:5490958/93‑1 (MQ=255)
      aCTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCAc                                                                                      >  2:5912298/1‑93 (MQ=255)
      aCTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCAc                                                                                      >  1:2579450/1‑93 (MQ=255)
          aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTc                                                                                  >  1:435148/1‑93 (MQ=255)
          aaCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCACATCATCGCCCATGTTGACGGAGGCCCGGTGCTGACCAGTCAGCAAGGCACCCTc                                                                                  >  1:3621959/1‑93 (MQ=255)
           aCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCa                                                                                 >  1:5066987/1‑93 (MQ=255)
           aCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCa                                                                                 <  2:2597679/93‑1 (MQ=255)
             cccGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAAc                                                                               <  1:2268501/93‑1 (MQ=255)
                ggCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGt                                                                            >  2:6170808/1‑93 (MQ=255)
                ggCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGt                                                                            <  2:3549238/93‑1 (MQ=255)
                ggCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGt                                                                            <  1:2708904/93‑1 (MQ=255)
                 gCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTc                                                                           <  2:2518673/93‑1 (MQ=255)
                  cGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCa                                                                          <  1:3808109/93‑1 (MQ=255)
                   gACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAg                                                                         <  1:5631462/93‑1 (MQ=255)
                   gACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAg                                                                         >  1:1847160/1‑93 (MQ=255)
                     cGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTa                                                                       <  2:161046/93‑1 (MQ=255)
                      gTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTAt                                                                      <  2:2360560/93‑1 (MQ=255)
                       tCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATg                                                                     <  1:3731390/93‑1 (MQ=255)
                         cTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCg                                                                   <  2:770255/93‑1 (MQ=255)
                         cTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCg                                                                   >  1:3340196/1‑93 (MQ=255)
                          tGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGt                                                                  <  2:5806777/93‑1 (MQ=255)
                          tGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGt                                                                  >  1:3229119/1‑93 (MQ=255)
                          tGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGt                                                                  >  1:6294846/1‑93 (MQ=255)
                           gCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGtt                                                                 <  2:2092938/93‑1 (MQ=255)
                            ccGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTa                                                                >  2:6429879/1‑93 (MQ=255)
                             cGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTAc                                                               <  2:4010310/93‑1 (MQ=255)
                              gATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACg                                                              <  2:1388701/93‑1 (MQ=255)
                              gATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACg                                                              >  1:2930606/1‑93 (MQ=255)
                              gATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACg                                                              <  2:1824544/93‑1 (MQ=255)
                              gATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACg                                                              >  1:4510339/1‑93 (MQ=255)
                              gATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACg                                                              >  2:3493754/1‑93 (MQ=255)
                               atatAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACgg                                                             <  1:6148501/93‑1 (MQ=255)
                               aTAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACgg                                                             >  2:1767260/1‑93 (MQ=255)
                               aTAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACgg                                                             >  1:5222584/1‑93 (MQ=255)
                                 aGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGAt                                                           <  1:1253408/93‑1 (MQ=255)
                                    aaaaCCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGa                                                        <  2:733861/93‑1 (MQ=255)
                                    aaaaCCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGa                                                        <  1:1314907/93‑1 (MQ=255)
                                     aaaCCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGaa                                                       <  2:6254512/93‑1 (MQ=255)
                                       aCCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACa                                                     >  2:2997469/1‑93 (MQ=255)
                                       aCCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACa                                                     <  2:4368277/93‑1 (MQ=255)
                                        cccGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACAt                                                    <  1:1105326/93‑1 (MQ=255)
                                         ccGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACAtt                                                   >  1:4422462/1‑93 (MQ=255)
                                         ccGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACAtt                                                   <  1:1040788/93‑1 (MQ=255)
                                          cGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACAttt                                                  <  2:6375256/93‑1 (MQ=255)
                                          cGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACAttt                                                  <  2:5069350/93‑1 (MQ=255)
                                           gATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTg                                                 >  1:1398952/1‑93 (MQ=255)
                                            aTCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGa                                                >  2:2738975/1‑93 (MQ=255)
                                            aTCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGa                                                <  2:3614298/93‑1 (MQ=255)
                                             tCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGAt                                               <  2:3195885/93‑1 (MQ=255)
                                             tCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGAt                                               <  1:210616/93‑1 (MQ=255)
                                              cGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGAtt                                              <  2:5266538/93‑1 (MQ=255)
                                              cGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAAAATTTGAtt                                              >  2:5158574/1‑93 (MQ=255)
                                               gCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTa                                             >  2:2394323/1‑93 (MQ=255)
                                                cgtcatcGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTaa                                            <  2:2490466/91‑1 (MQ=255)
                                                 atcatcGCACATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAAc                                           >  1:6490050/1‑93 (MQ=255)
                                                  tcatcGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAAcc                                          <  2:4911756/93‑1 (MQ=255)
                                                    atcGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCg                                        <  2:3334771/93‑1 (MQ=255)
                                                    atcGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCg                                        >  1:2835181/1‑93 (MQ=255)
                                                      cGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAt                                      >  1:4839072/1‑93 (MQ=255)
                                                       gCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAtt                                     >  2:709744/1‑93 (MQ=255)
                                                       gCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAtt                                     <  2:2821930/93‑1 (MQ=255)
                                                        cccATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAttt                                    >  1:720103/1‑93 (MQ=255)
                                                         ccATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAtttt                                   >  2:4554795/1‑93 (MQ=255)
                                                         ccATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAtttt                                   >  2:5524023/1‑93 (MQ=255)
                                                          cATGTTGATGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTa                                  >  2:3688513/1‑93 (MQ=255)
                                                          cATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTa                                  >  1:241620/1‑93 (MQ=255)
                                                           aTGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTaa                                 >  2:2655639/1‑93 (MQ=255)
                                                             gTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAAt                               >  2:2077746/1‑93 (MQ=255)
                                                              ttGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAAtt                              >  2:917496/1‑93 (MQ=255)
                                                              ttGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAAtt                              >  1:1134090/1‑93 (MQ=255)
                                                               tGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATtc                             >  1:4871724/1‑93 (MQ=255)
                                                                 aCGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATtctc                           >  1:1874354/1‑93 (MQ=255)
                                                                  cGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTctct                          <  2:4391509/93‑1 (MQ=255)
                                                                    gCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGCACATTTGATTAACCCGATTTTAAATTCTCTGa                        >  1:2769803/1‑93 (MQ=255)
                                                                     cGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGaa                       >  2:1655547/1‑93 (MQ=255)
                                                                        cccGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGa                    <  1:1002108/93‑1 (MQ=255)
                                                                        cccGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGa                    >  2:42535/1‑93 (MQ=255)
                                                                          cGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAgg                  >  1:5807330/1‑93 (MQ=255)
                                                                           ggTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGa                 >  2:6544990/1‑93 (MQ=255)
                                                                           ggTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGa                 >  1:2178175/1‑93 (MQ=255)
                                                                             tGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAAc               <  1:671543/93‑1 (MQ=255)
                                                                             tGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAAc               <  1:4666619/93‑1 (MQ=255)
                                                                              gCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACa              <  1:3952425/93‑1 (MQ=255)
                                                                               cTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACACGAGTTTAAATTCTCTGAACGAGGAACat             >  2:1170983/1‑92 (MQ=255)
                                                                                tGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGc            <  2:2185790/93‑1 (MQ=255)
                                                                                tGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGc            >  2:1037427/1‑93 (MQ=255)
                                                                                 gACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGcc           <  1:5368789/93‑1 (MQ=255)
                                                                                  aCCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGccc          >  1:593178/1‑93 (MQ=255)
                                                                                   ccAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCa         <  2:1972086/93‑1 (MQ=255)
                                                                                    cagtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCaa        >  2:1862332/1‑93 (MQ=255)
                                                                                    cagtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAAGAACAGCCCaa        >  2:5377287/1‑93 (MQ=255)
                                                                                     agtcagtATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCaaa       <  2:286942/93‑1 (MQ=255)
                                                                                     agtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCaaa       <  2:4774117/93‑1 (MQ=255)
                                                                                      gtcagtATGGCACCCTCAACGGTCAGTATGCGTCACGGATAGAACATTTGATTACCCCGATTTTAAATCCTCTGAACGTGGAACAGCCCAAAt      <  1:1560854/93‑1 (MQ=255)
                                                                                      gtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAt      <  2:5608790/93‑1 (MQ=255)
                                                                                      gtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAt      >  1:4053590/1‑93 (MQ=255)
                                                                                      gtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAt      <  1:2500517/93‑1 (MQ=255)
                                                                                      gtcagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAt      <  2:6622283/93‑1 (MQ=255)
                                                                                        cagcATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGa    <  2:4119714/93‑1 (MQ=255)
                                                                                           |                                                                                           
TTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGT  >  NC_000913/2018933‑2019115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGA  >  NC_000913/2018931‑2019117
                                                                                             |                                                                                             
ATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGT                                                                                               <  SRR4302547.4410089/94‑1 (MQ=60)
ATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGC                                                                                               >  SRR4302547.1691047/1‑94 (MQ=60)
 TTTTAAACCTGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCA                                                                                              <  SRR4302547.987923/94‑1 (MQ=60)
  TTTAAAACTGACCGCCGCAGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCAT                                                                                             <  SRR4302547.4444814/94‑1 (MQ=60)
  TTTAAAACTGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCAT                                                                                             <  SRR4302547.3397758/94‑1 (MQ=60)
  TTTAAAACTGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCAT                                                                                             <  SRR4302547.4014289/94‑1 (MQ=60)
  TTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGGAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGGCAGCAT                                                                                             >  SRR4302547.603775/1‑94 (MQ=60)
    TAAAACTGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGG                                                                                           <  SRR4302547.3146417/94‑1 (MQ=60)
      AAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGAGCTCATCATCGCCCATGCTGAGCGCGGCCCGGTGCCGACCACTCAGCATGGCA                                                                                         >  SRR4302547.1869005/1‑94 (MQ=53)
       AACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCAC                                                                                        >  SRR4302547.2631504/1‑94 (MQ=60)
        ACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACC                                                                                       <  SRR4302547.5604339/94‑1 (MQ=60)
          TGACCCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCT                                                                                     <  SRR4302547.300114/94‑1 (MQ=60)
           GAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTC                                                                                    >  SRR4302547.443206/1‑94 (MQ=60)
           GAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCACATCATCGCCCATGTTGACGGAGGCCCGGTGCTGACCAGTCAGCAAGGCACCCTC                                                                                    >  SRR4302547.3695843/1‑94 (MQ=60)
            AACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCA                                                                                   >  SRR4302547.6332936/1‑94 (MQ=60)
            AACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCA                                                                                   >  SRR4302547.4343664/1‑94 (MQ=60)
            AACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCA                                                                                   >  SRR4302547.5171535/1‑94 (MQ=60)
            AACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGCCAGCATGGCACCCTCA                                                                                   >  SRR4302547.3721329/1‑94 (MQ=60)
             ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCTCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCAGCCTCAA                                                                                  >  SRR4302547.6455338/1‑94 (MQ=60)
               CCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACG                                                                                >  SRR4302547.1304395/1‑94 (MQ=60)
               CCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACG                                                                                <  SRR4302547.2314377/94‑1 (MQ=60)
               CCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACG                                                                                >  SRR4302547.2878698/1‑94 (MQ=60)
                 CGGCGAGGTCCTGCCGATAGAAAAACCCGATCGCATCAACGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCCCCCTCAACGGT                                                                              <  SRR4302547.4568861/94‑1 (MQ=60)
                  GGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTC                                                                             >  SRR4302547.2972648/1‑94 (MQ=60)
                  GGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTC                                                                             <  SRR4302547.2763288/94‑1 (MQ=60)
                  GGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTC                                                                             >  SRR4302547.1007270/1‑94 (MQ=60)
                   GCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCA                                                                            >  SRR4302547.4053250/1‑94 (MQ=60)
                    CGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAG                                                                           >  SRR4302547.1883678/1‑94 (MQ=60)
                    CGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAG                                                                           <  SRR4302547.3885729/94‑1 (MQ=60)
                     GCCGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGCCCAGTCAGCATGGCCCCCTCAACGGTCAGT                                                                          <  SRR4302547.4947741/94‑1 (MQ=60)
                     GACGTCCTGCCGATAGAAAACCCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCTCCCTCAACGGTCAGT                                                                          <  SRR4302547.252351/94‑1 (MQ=60)
                     GACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGT                                                                          <  SRR4302547.1242367/94‑1 (MQ=60)
                     GACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGT                                                                          >  SRR4302547.4339273/1‑94 (MQ=60)
                     GACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGT                                                                          <  SRR4302547.5747682/94‑1 (MQ=60)
                     GACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCAATGTTGACGCCGTCCCGGTGCCGACCAGTCAGCATGGCAACCTCACCGGTCAGT                                                                          >  SRR4302547.1597145/1‑94 (MQ=60)
                       CGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTAT                                                                        >  SRR4302547.1213702/1‑94 (MQ=60)
                        GTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATG                                                                       <  SRR4302547.2725219/94‑1 (MQ=60)
                        CTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATG                                                                       <  SRR4302547.4447198/94‑1 (MQ=60)
                         TCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGC                                                                      <  SRR4302547.3807576/94‑1 (MQ=60)
                          CCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCG                                                                     >  SRR4302547.3407794/1‑94 (MQ=60)
                           CTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGT                                                                    >  SRR4302547.3293562/1‑94 (MQ=60)
                           CTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGT                                                                    >  SRR4302547.6425702/1‑94 (MQ=60)
                           CTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGT                                                                    >  SRR4302547.3677311/1‑94 (MQ=60)
                            TGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCACCGGTCAGTATGCGTT                                                                   >  SRR4302547.4867998/1‑94 (MQ=60)
                            TGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTT                                                                   <  SRR4302547.2166628/94‑1 (MQ=60)
                             GCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTA                                                                  >  SRR4302547.5805695/1‑94 (MQ=60)
                             GCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTA                                                                  <  SRR4302547.6649915/94‑1 (MQ=60)
                              CCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTGAACGGTCAGTATGCGTTAC                                                                 >  SRR4302547.383072/1‑94 (MQ=60)
                               CGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACG                                                                >  SRR4302547.5617542/1‑94 (MQ=60)
                               CGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACG                                                                >  SRR4302547.2989021/1‑94 (MQ=60)
                               CGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACG                                                                >  SRR4302547.4603521/1‑94 (MQ=60)
                               CGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACG                                                                >  SRR4302547.4704903/1‑94 (MQ=60)
                               CGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACG                                                                >  SRR4302547.2454389/1‑94 (MQ=60)
                                GATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGG                                                               >  SRR4302547.5330052/1‑94 (MQ=60)
                                 ATATAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGA                                                              <  SRR4302547.6276060/94‑1 (MQ=60)
                                 ATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGA                                                              >  SRR4302547.2804188/1‑94 (MQ=60)
                                 ATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGA                                                              >  SRR4302547.1101920/1‑94 (MQ=60)
                                 ATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGA                                                              >  SRR4302547.2478869/1‑94 (MQ=60)
                                   AGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATA                                                            <  SRR4302547.1278692/94‑1 (MQ=60)
                                      AAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAA                                                         <  SRR4302547.1341338/94‑1 (MQ=60)
                                      AAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAA                                                         <  SRR4302547.1884399/94‑1 (MQ=60)
                                       AAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAAC                                                        <  SRR4302547.2778803/94‑1 (MQ=60)
                                        AACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACA                                                       <  SRR4302547.3347619/94‑1 (MQ=60)
                                        AACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACA                                                       <  SRR4302547.5123778/94‑1 (MQ=60)
                                         ACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACAT                                                      >  SRR4302547.839945/1‑94 (MQ=60)
                                          CCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATT                                                     <  SRR4302547.1127565/94‑1 (MQ=60)
                                          CCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATT                                                     >  SRR4302547.4513812/1‑94 (MQ=60)
                                           CCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTT                                                    <  SRR4302547.1061308/94‑1 (MQ=60)
                                           CCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTT                                                    <  SRR4302547.2284509/94‑1 (MQ=60)
                                            CGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTG                                                   >  SRR4302547.1426941/1‑94 (MQ=60)
                                             GATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGT                                                  >  SRR4302547.1397795/1‑94 (MQ=60)
                                              ATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGAT                                                 <  SRR4302547.6147031/94‑1 (MQ=60)
                                              ATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGAT                                                 <  SRR4302547.5489061/94‑1 (MQ=60)
                                               TCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATT                                                <  SRR4302547.214583/94‑1 (MQ=60)
                                               TCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATT                                                <  SRR4302547.1591309/94‑1 (MQ=60)
                                               TCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATT                                                <  SRR4302547.4669904/94‑1 (MQ=60)
                                                  CATCATCGCACATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAAC                                             >  SRR4302547.6625164/1‑94 (MQ=60)
                                                     CATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCG                                          <  SRR4302547.3887844/94‑1 (MQ=60)
                                                     CATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCG                                          >  SRR4302547.2891867/1‑94 (MQ=60)
                                                      ATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGA                                         <  SRR4302547.4819525/94‑1 (MQ=60)
                                                       TCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAT                                        >  SRR4302547.4939373/1‑94 (MQ=60)
                                                       TCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGAT                                        >  SRR4302547.2185260/1‑94 (MQ=60)
                                                         GCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTT                                      >  SRR4302547.733498/1‑94 (MQ=60)
                                                         GCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTT                                      <  SRR4302547.3955781/94‑1 (MQ=60)
                                                          CCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTT                                     <  SRR4302547.5228461/94‑1 (MQ=60)
                                                          CCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTT                                     >  SRR4302547.1295048/1‑94 (MQ=60)
                                                           CCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTA                                    >  SRR4302547.246145/1‑94 (MQ=60)
                                                             ATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAA                                  >  SRR4302547.1563878/1‑94 (MQ=60)
                                                             ATGTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAA                                  >  SRR4302547.1139252/1‑94 (MQ=60)
                                                               GTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATT                                >  SRR4302547.4967276/1‑94 (MQ=60)
                                                               GTTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATT                                >  SRR4302547.1156997/1‑94 (MQ=60)
                                                                TTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTC                               >  SRR4302547.4972678/1‑94 (MQ=60)
                                                                TTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTC                               <  SRR4302547.5207830/94‑1 (MQ=60)
                                                                TTGACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTC                               <  SRR4302547.6198592/94‑1 (MQ=60)
                                                                 TGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCT                              >  SRR4302547.5643136/1‑94 (MQ=60)
                                                                  GACGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTC                             >  SRR4302547.1911383/1‑94 (MQ=60)
                                                                    CGGCTTCCCGGTGCTGACTAGTCAGCATGGCACCCTCAACGGTGAGTATGGGTTTCGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTG                           <  SRR4302547.1413217/94‑1 (MQ=60)
                                                                    CGGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTG                           >  SRR4302547.2713134/1‑94 (MQ=60)
                                                                     GGCGTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGCACATTTGATTAACCCGATTTTAAATTCTCTGA                          >  SRR4302547.2825298/1‑94 (MQ=60)
                                                                        GTCCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACG                       <  SRR4302547.6528639/94‑1 (MQ=60)
                                                                         TCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGA                      >  SRR4302547.5142631/1‑94 (MQ=60)
                                                                          CCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAG                     <  SRR4302547.1021658/94‑1 (MQ=60)
                                                                          CCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAG                     <  SRR4302547.1063378/94‑1 (MQ=60)
                                                                          CCCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAG                     <  SRR4302547.2899579/94‑1 (MQ=60)
                                                                           CCGGTGCTGACCAGTCATCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTTACCCGATTTTAAATTCTCTGAACGAGG                    >  SRR4302547.5575751/1‑94 (MQ=60)
                                                                           CCGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGG                    >  SRR4302547.5927469/1‑94 (MQ=60)
                                                                            CGGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGA                   >  SRR4302547.2221792/1‑94 (MQ=60)
                                                                             GGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAA                  >  SRR4302547.3497607/1‑94 (MQ=60)
                                                                             GGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAA                  >  SRR4302547.1798467/1‑94 (MQ=60)
                                                                             GGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAA                  >  SRR4302547.1057160/1‑94 (MQ=60)
                                                                             CGTGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAA                  <  SRR4302547.5401247/94‑1 (MQ=60)
                                                                               TGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACA                <  SRR4302547.443394/94‑1 (MQ=60)
                                                                               TGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACA                <  SRR4302547.683904/94‑1 (MQ=60)
                                                                               TGCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACA                <  SRR4302547.4763260/94‑1 (MQ=60)
                                                                                GCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAG               <  SRR4302547.718176/94‑1 (MQ=60)
                                                                                GCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAG               <  SRR4302547.4032806/94‑1 (MQ=60)
                                                                                GCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAG               >  SRR4302547.712282/1‑94 (MQ=60)
                                                                                GCTGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAG               >  SRR4302547.2436124/1‑94 (MQ=60)
                                                                                  TGACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCC             <  SRR4302547.6052577/94‑1 (MQ=60)
                                                                                   GACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCC            >  SRR4302547.604013/1‑94 (MQ=60)
                                                                                   GACCAGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCC            <  SRR4302547.5479586/94‑1 (MQ=60)
                                                                                       AGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAT        <  SRR4302547.2602295/94‑1 (MQ=60)
                                                                                       AGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAT        >  SRR4302547.2034258/1‑94 (MQ=60)
                                                                                       AGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAT        <  SRR4302547.1972484/94‑1 (MQ=60)
                                                                                       AGTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAT        >  SRR4302547.4135910/1‑94 (MQ=60)
                                                                                        GTCAGTATGGCACCCTCAACGGTCAGTATGCGTCACGGATAGAACATTTGATTACCCCGATTTTAAATCCTCTGAACGTGGAACAGCCCAAATG       <  SRR4302547.1591948/94‑1 (MQ=60)
                                                                                        GTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTTAACGAGGAACAGCCCAAATG       <  SRR4302547.6547161/94‑1 (MQ=60)
                                                                                        GTCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATG       <  SRR4302547.2551033/94‑1 (MQ=60)
                                                                                         TCAGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGA      >  SRR4302547.3882790/1‑94 (MQ=60)
                                                                                           AGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGT    <  SRR4302547.2220695/94‑1 (MQ=60)
                                                                                           AGCATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGT    >  SRR4302547.256176/1‑94 (MQ=60)
                                                                                            GTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTG   <  SRR4302547.1646238/94‑1 (MQ=60)
                                                                                             CATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGA  >  SRR4302547.3379394/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGA  >  NC_000913/2018931‑2019117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑