Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,026,185 C→T intergenic (‑131/+25) b1956 ← / ← b1957 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,026,1850CT84.8% 546.4 / 70.1 197intergenic (‑131/+25)b1956/b1957hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/13);  new base T (98/69);  total (115/82)
Fisher's exact test for biased strand distribution p-value = 8.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 198 total aligned reads displayed.

GTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCC  >  NC_000913/2026093‑2026273
                                                                                            |                                                                                        
  aCCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCg                                                                                        <  2:165203/93‑1 (MQ=255)
  aCCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCg                                                                                        <  1:2665262/93‑1 (MQ=255)
    cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                      <  2:2653467/93‑1 (MQ=255)
    cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGACGGATGTGATTCGGc                                                                                      >  2:1614022/1‑93 (MQ=255)
     gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGcc                                                                                     >  2:139890/1‑93 (MQ=255)
     gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGcc                                                                                     >  2:3743109/1‑93 (MQ=255)
       aaaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTg                                                                                   >  1:2295156/1‑93 (MQ=255)
          aCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATg                                                                                <  1:5740452/93‑1 (MQ=255)
           cAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGt                                                                               >  2:1154807/1‑93 (MQ=255)
            aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGtt                                                                              <  1:2952502/93‑1 (MQ=255)
            aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGtt                                                                              >  1:4876474/1‑93 (MQ=255)
            aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGtt                                                                              <  2:1361462/93‑1 (MQ=255)
            aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGtt                                                                              >  2:5354117/1‑93 (MQ=255)
             gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTa                                                                             >  2:5464975/1‑93 (MQ=255)
             gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTa                                                                             >  1:544136/1‑93 (MQ=255)
                  aaTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGAt                                                                        >  1:5811907/1‑93 (MQ=255)
                    ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATAc                                                                      <  1:5415474/93‑1 (MQ=255)
                    ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATAc                                                                      >  2:5640252/1‑93 (MQ=255)
                     tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATAcc                                                                     >  2:111265/1‑93 (MQ=255)
                      ttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATAccc                                                                    >  1:753425/1‑93 (MQ=255)
                      ttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATAccc                                                                    <  2:2717335/93‑1 (MQ=255)
                       tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCt                                                                   >  1:1350405/1‑93 (MQ=255)
                       tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCt                                                                   <  2:6625622/93‑1 (MQ=255)
                         aaaTGGTGGCATGATAAGACGCAACTATCACTCATTGCAGCCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCttt                                                                 <  1:4678570/92‑1 (MQ=255)
                          aaTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTc                                                                >  2:4730961/1‑93 (MQ=255)
                          aaTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTc                                                                >  2:3629541/1‑93 (MQ=255)
                           aTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTca                                                               >  1:325941/1‑93 (MQ=255)
                            tggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTcac                                                              >  2:1239771/1‑93 (MQ=255)
                            tggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTcac                                                              >  1:6162515/1‑93 (MQ=255)
                              gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCacac                                                            >  1:273138/1‑93 (MQ=255)
                               tggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACAct                                                           <  2:1062954/93‑1 (MQ=255)
                                ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACActt                                                          <  1:2016098/93‑1 (MQ=255)
                                ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACActt                                                          >  2:3085477/1‑93 (MQ=255)
                                ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACActt                                                          >  2:127080/1‑93 (MQ=255)
                                ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACActt                                                          <  1:4065019/93‑1 (MQ=255)
                                 gCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACActtc                                                         >  2:4347631/1‑93 (MQ=255)
                                  cATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACActtct                                                        >  2:3931273/1‑93 (MQ=255)
                                    tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACttcttc                                                      >  2:4871457/1‑93 (MQ=255)
                                    tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACttcttc                                                      >  1:2545121/1‑93 (MQ=255)
                                    tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACttcttc                                                      >  2:6089996/1‑93 (MQ=255)
                                    tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACttcttc                                                      <  1:2991717/93‑1 (MQ=255)
                                     gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                     >  2:5599808/1‑93 (MQ=255)
                                     gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                     >  1:5279078/1‑93 (MQ=255)
                                     gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGAGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                     <  1:5454902/93‑1 (MQ=255)
                                     gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACAACAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                     >  1:1026130/1‑93 (MQ=255)
                                      aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCg                                                    >  1:4145097/1‑93 (MQ=255)
                                      aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCg                                                    <  1:2028253/93‑1 (MQ=255)
                                       tAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                   >  2:2492753/1‑93 (MQ=255)
                                       tAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                   >  2:4115908/1‑93 (MQ=255)
                                       tAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                   >  2:145783/1‑93 (MQ=255)
                                         aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAg                                                 >  1:417903/1‑93 (MQ=255)
                                          gACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGa                                                >  1:1962610/1‑93 (MQ=255)
                                               gACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAc                                           >  1:2504431/1‑93 (MQ=255)
                                                aCTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                          <  1:1233428/93‑1 (MQ=255)
                                                aCTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                          >  2:2000204/1‑93 (MQ=255)
                                                 cTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTAATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActc                                         <  2:4663631/93‑1 (MQ=255)
                                                  tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                        >  2:3302065/1‑93 (MQ=255)
                                                  tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                        <  2:2854304/93‑1 (MQ=255)
                                                   aTCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActccc                                       <  1:3305591/93‑1 (MQ=255)
                                                    tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccc                                      >  2:3963722/1‑93 (MQ=255)
                                                    tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccc                                      <  2:4196550/93‑1 (MQ=255)
                                                    tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccc                                      <  2:6494774/93‑1 (MQ=255)
                                                     cattcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccct                                     >  2:2142050/1‑93 (MQ=255)
                                                      attcattGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctc                                    >  2:5764390/1‑93 (MQ=255)
                                                       ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctcc                                   >  2:6054344/1‑93 (MQ=255)
                                                        tcattGCAGGTATTATCACAGCAGGCCGGATGCTATTCGGCCTGATGTTAATGATACCCATTCACACTTCTTCGGCAGGACGActcccctccc                                  >  1:5586429/1‑93 (MQ=255)
                                                        tcattGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                  >  2:2416869/1‑93 (MQ=255)
                                                         cattGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTTACACTTCTTCCGCAGAACGACtcccctcccc                                 >  1:2861775/1‑93 (MQ=255)
                                                          attGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                >  2:2691385/1‑93 (MQ=255)
                                                           ttGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATCCCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCg                               <  1:3505508/93‑1 (MQ=255)
                                                           ttGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCg                               <  1:4399470/93‑1 (MQ=255)
                                                           ttGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCg                               <  2:4515551/93‑1 (MQ=255)
                                                            tGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCgg                              >  1:3678747/1‑93 (MQ=255)
                                                               aGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                           <  1:5009740/93‑1 (MQ=255)
                                                               aGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                           >  2:5943035/1‑93 (MQ=255)
                                                                  cATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAg                        <  2:5535437/93‑1 (MQ=255)
                                                                   aTTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGc                       >  2:2261209/1‑93 (MQ=255)
                                                                   aTTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGc                       >  1:1628109/1‑93 (MQ=255)
                                                                     tAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                     <  2:3449649/93‑1 (MQ=255)
                                                                        cacaCCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGt                  <  1:6616543/93‑1 (MQ=255)
                                                                         acacCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCGCACTTCTTCCGCAGAACCACTCCCCTCCCCCGGAACAAGCTCGGtt                 <  1:3927102/93‑1 (MQ=255)
                                                                             cAGTCCGGATGTGATCCGGCCTGATGCTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCCGAACAAGCTCGGTTTcgt             >  1:657858/1‑91 (MQ=255)
                                                                             cAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt             <  2:5879245/93‑1 (MQ=255)
                                                                               ggCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGa           >  2:3239317/1‑93 (MQ=255)
                                                                               ggCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGa           >  2:6269176/1‑93 (MQ=255)
                                                                               ggCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGa           >  1:4710663/1‑93 (MQ=255)
                                                                               ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGa           >  1:3353791/1‑93 (MQ=255)
                                                                                 ccGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa         <  1:1010368/93‑1 (MQ=255)
                                                                                  cGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaaa        >  2:3696267/1‑93 (MQ=255)
                                                                                   ggATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg       <  2:3587520/93‑1 (MQ=255)
                                                                                    gATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc      >  1:6427715/1‑93 (MQ=255)
                                                                                    gATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc      <  1:1494012/93‑1 (MQ=255)
                                                                                    gATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc      >  1:3088506/1‑93 (MQ=255)
                                                                                    gATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc      >  1:5961320/1‑93 (MQ=255)
                                                                                     aTGTGATTCGGCCTGATGTTAATGATGCCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCt     <  2:4992510/93‑1 (MQ=255)
                                                                                     aTGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCt     >  1:4339567/1‑93 (MQ=255)
                                                                                      tgtgATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCtt    >  1:875541/1‑93 (MQ=255)
                                                                                       gtgATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTc   >  1:4791623/1‑93 (MQ=255)
                                                                                        tgATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc  >  1:4990694/1‑93 (MQ=255)
                                                                                        tgATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc  <  1:5354117/93‑1 (MQ=255)
                                                                                            |                                                                                        
GTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCC  >  NC_000913/2026093‑2026273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTT  >  NC_000913/2026094‑2026278
                                                                                           |                                                                                             
TACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCG                                                                                             >  SRR4302547.5834925/1‑94 (MQ=60)
TACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCG                                                                                             <  SRR4302547.1509266/94‑1 (MQ=60)
 ACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGG                                                                                            <  SRR4302547.2718818/94‑1 (MQ=60)
    GCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCT                                                                                         <  SRR4302547.4936128/94‑1 (MQ=60)
     CAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTG                                                                                        >  SRR4302547.2341664/1‑94 (MQ=60)
     CAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTG                                                                                        >  SRR4302547.654029/1‑94 (MQ=60)
       AAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGAT                                                                                      <  SRR4302547.3122847/94‑1 (MQ=60)
         ACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGT                                                                                    <  SRR4302547.198977/94‑1 (MQ=60)
         ACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGT                                                                                    <  SRR4302547.5859081/94‑1 (MQ=60)
          CAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTT                                                                                   >  SRR4302547.4977506/1‑94 (MQ=60)
           AGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTA                                                                                  <  SRR4302547.3011284/94‑1 (MQ=60)
           AGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTA                                                                                  <  SRR4302547.5523094/94‑1 (MQ=60)
           AGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTA                                                                                  <  SRR4302547.5528316/94‑1 (MQ=60)
           AGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTA                                                                                  >  SRR4302547.553959/1‑94 (MQ=60)
            GCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAA                                                                                 >  SRR4302547.1300921/1‑94 (MQ=60)
             CGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAAT                                                                                >  SRR4302547.1218291/1‑94 (MQ=60)
              GATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATG                                                                               <  SRR4302547.1454532/94‑1 (MQ=60)
              GATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATG                                                                               <  SRR4302547.3811618/94‑1 (MQ=60)
               ATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGA                                                                              <  SRR4302547.2259161/94‑1 (MQ=60)
                TAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGAT                                                                             >  SRR4302547.5932152/1‑94 (MQ=60)
                 AATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATA                                                                            <  SRR4302547.508644/94‑1 (MQ=60)
                   TTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACC                                                                          <  SRR4302547.5527510/94‑1 (MQ=60)
                    TTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCC                                                                         >  SRR4302547.767629/1‑94 (MQ=60)
                     TTATAATGGTGGCATGATAAGACGCGACTATTATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCT                                                                        <  SRR4302547.2568441/94‑1 (MQ=60)
                     TTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCT                                                                        >  SRR4302547.1377531/1‑94 (MQ=60)
                      TATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTT                                                                       >  SRR4302547.5319229/1‑94 (MQ=60)
                      TATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTT                                                                       <  SRR4302547.2628618/94‑1 (MQ=60)
                        TAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTC                                                                     <  SRR4302547.696485/94‑1 (MQ=60)
                        AAATGGTGGCATGATAAGACGCAACTATCACTCATTGCAGCCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTC                                                                     <  SRR4302547.4775483/94‑1 (MQ=60)
                         AATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCA                                                                    <  SRR4302547.3372851/94‑1 (MQ=60)
                         AATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCA                                                                    >  SRR4302547.332008/1‑94 (MQ=60)
                          ATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCAC                                                                   >  SRR4302547.6290412/1‑94 (MQ=60)
                            GGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACAC                                                                 >  SRR4302547.278245/1‑94 (MQ=60)
                            GGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACAC                                                                 <  SRR4302547.5556584/94‑1 (MQ=60)
                             GTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACT                                                                >  SRR4302547.2481087/1‑94 (MQ=60)
                               GGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTC                                                              <  SRR4302547.2056212/94‑1 (MQ=60)
                               GGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTC                                                              <  SRR4302547.4147581/94‑1 (MQ=60)
                                  ATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTC                                                           >  SRR4302547.4394121/1‑94 (MQ=60)
                                  ATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTC                                                           >  SRR4302547.2596523/1‑94 (MQ=60)
                                  ATGATAAGACGCGACTATCATTCAGTGCAGGCATTAGCACACCAGGCTGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTC                                                           >  SRR4302547.5449722/1‑94 (MQ=60)
                                   TGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCC                                                          >  SRR4302547.5387967/1‑94 (MQ=60)
                                   TGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCC                                                          <  SRR4302547.3051232/94‑1 (MQ=60)
                                   TGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACAACAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCC                                                          >  SRR4302547.1046274/1‑94 (MQ=60)
                                    GATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCG                                                         >  SRR4302547.4229471/1‑94 (MQ=60)
                                    GATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGAGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCG                                                         <  SRR4302547.5567614/94‑1 (MQ=60)
                                     ATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGC                                                        <  SRR4302547.2068640/94‑1 (MQ=60)
                                     ATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGC                                                        <  SRR4302547.4071445/94‑1 (MQ=60)
                                       AAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAG                                                      >  SRR4302547.303609/1‑94 (MQ=60)
                                       AAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAG                                                      >  SRR4302547.425649/1‑94 (MQ=60)
                                        AGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGA                                                     >  SRR4302547.2001573/1‑94 (MQ=60)
                                        AGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGA                                                     >  SRR4302547.2007964/1‑94 (MQ=60)
                                          ACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAAC                                                   >  SRR4302547.2993727/1‑94 (MQ=60)
                                             CGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAC                                                >  SRR4302547.2555039/1‑94 (MQ=60)
                                              GACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACT                                               >  SRR4302547.4477772/1‑94 (MQ=60)
                                              GACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACT                                               >  SRR4302547.4776320/1‑94 (MQ=60)
                                               ACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTC                                              <  SRR4302547.1258317/94‑1 (MQ=60)
                                                CTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCC                                             >  SRR4302547.3491075/1‑94 (MQ=60)
                                                CTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCC                                             <  SRR4302547.847131/94‑1 (MQ=60)
                                                CTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCC                                             >  SRR4302547.205631/1‑94 (MQ=60)
                                                CTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCC                                             <  SRR4302547.5997210/94‑1 (MQ=60)
                                                  ATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCC                                           <  SRR4302547.3372202/94‑1 (MQ=60)
                                                   TCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCT                                          <  SRR4302547.4245156/94‑1 (MQ=60)
                                                      TTCATTGCAGGTATTATCACAGCAGGCCGGATGCTATTCGGCCTGATGTTAATGATACCCATTCACACTTCTTCGGCAGGACGACTCCCCTCCC                                       >  SRR4302547.5701658/1‑94 (MQ=53)
                                                       TCATTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTTACACTTCTTCCGCAGAACGACTCCCCTCCCC                                      >  SRR4302547.2918945/1‑94 (MQ=60)
                                                          TTGCAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGG                                   >  SRR4302547.3753854/1‑94 (MQ=60)
                                                          TTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATCCCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGG                                   <  SRR4302547.3577053/94‑1 (MQ=60)
                                                          TTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGG                                   <  SRR4302547.4490057/94‑1 (MQ=60)
                                                             CAGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAC                                <  SRR4302547.3729707/94‑1 (MQ=60)
                                                              AGGCATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACA                               <  SRR4302547.5113313/94‑1 (MQ=60)
                                                              AGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACA                               >  SRR4302547.5048089/1‑94 (MQ=60)
                                                                 CATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGC                            >  SRR4302547.1660319/1‑94 (MQ=60)
                                                                  ATTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCT                           <  SRR4302547.3159071/94‑1 (MQ=60)
                                                                   TTAGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTC                          <  SRR4302547.3366838/94‑1 (MQ=60)
                                                                     AGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGG                        >  SRR4302547.6582854/1‑94 (MQ=60)
                                                                     AGCACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGG                        <  SRR4302547.6722595/94‑1 (MQ=60)
                                                                       CACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTT                      <  SRR4302547.6754260/94‑1 (MQ=60)
                                                                        ACACCAGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCGCACTTCTTCCGCAGAACCACTCCCCTCCCCCGGAACAAGCTCGGTTT                     <  SRR4302547.4006975/94‑1 (MQ=60)
                                                                           CCAGTCCGGATGTGATCCGGCCTGATGCTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCCGAACAAGCTCGGTTTCGT                  >  SRR4302547.669975/1‑94 (MQ=60)
                                                                             AGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGA                >  SRR4302547.4808206/1‑94 (MQ=60)
                                                                             AGGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGA                >  SRR4302547.5700563/1‑94 (MQ=60)
                                                                             AGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGA                >  SRR4302547.3421809/1‑94 (MQ=60)
                                                                              GGCCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAA               >  SRR4302547.2764242/1‑94 (MQ=60)
                                                                                CCGGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAA             <  SRR4302547.1030110/94‑1 (MQ=60)
                                                                                  GGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGC           >  SRR4302547.279559/1‑94 (MQ=60)
                                                                                  GGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGC           >  SRR4302547.4831613/1‑94 (MQ=60)
                                                                                  GGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGC           >  SRR4302547.6561715/1‑94 (MQ=60)
                                                                                  GGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGC           >  SRR4302547.3149772/1‑94 (MQ=60)
                                                                                  GGATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGC           >  SRR4302547.6163412/1‑94 (MQ=60)
                                                                                  GGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGC           >  SRR4302547.6084941/1‑94 (MQ=60)
                                                                                   GATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCT          <  SRR4302547.1523815/94‑1 (MQ=60)
                                                                                   GATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCT          >  SRR4302547.4428815/1‑94 (MQ=60)
                                                                                    ATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTT         >  SRR4302547.892346/1‑94 (MQ=60)
                                                                                    ATGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTT         <  SRR4302547.4182579/94‑1 (MQ=60)
                                                                                     TGTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTC        >  SRR4302547.4890906/1‑94 (MQ=60)
                                                                                      GTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCC       >  SRR4302547.5093857/1‑94 (MQ=60)
                                                                                      GTGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCC       >  SRR4302547.473734/1‑94 (MQ=60)
                                                                                      GTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCC       <  SRR4302547.1615825/94‑1 (MQ=60)
                                                                                       TGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCC      <  SRR4302547.5865798/94‑1 (MQ=60)
                                                                                       TGATTCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCC      <  SRR4302547.5464523/94‑1 (MQ=60)
                                                                                          TGCGGCCTGATGTTAATTATACCCTTTCACACATCTTCCTCAGAACGACTCGCCTCCCCCGGAAGAAGCTCGGTTTCATGAAAAGCTTCCCGCT   <  SRR4302547.2891243/94‑1 (MQ=60)
                                                                                           TCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTT  >  SRR4302547.3598854/1‑94 (MQ=60)
                                                                                           |                                                                                             
TACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTT  >  NC_000913/2026094‑2026278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑