Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,080,143 C→T G145S (GGT→AGT)  dacD ← penicillin binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,080,1430CT87.2% 594.9 / 59.6 203G145S (GGT→AGT) dacDpenicillin binding protein 6b
Reads supporting (aligned to +/- strand):  ref base C (13/13);  new base T (88/89);  total (101/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

Only 100 of 208 total aligned reads displayed.

ATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGC  >  NC_000913/2080051‑2080232
                                                                                            |                                                                                         
aTCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCAcc                                                                                           >  2:4137844/1‑93 (MQ=255)
 tCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCg                                                                                          >  2:4694677/1‑93 (MQ=255)
  ccAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTgg                                                                                         <  2:71299/93‑1 (MQ=255)
  ccAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTgg                                                                                         <  1:4031126/93‑1 (MQ=255)
   cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGc                                                                                        <  2:590604/93‑1 (MQ=255)
    aGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCa                                                                                       >  1:4853213/1‑93 (MQ=255)
    aGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCa                                                                                       <  2:2347048/93‑1 (MQ=255)
     gACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCaa                                                                                      <  1:3163681/93‑1 (MQ=255)
     gACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCaa                                                                                      <  2:2515165/93‑1 (MQ=255)
      aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAat                                                                                     >  1:1996725/1‑93 (MQ=255)
      aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAat                                                                                     <  1:3969833/93‑1 (MQ=255)
      aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAat                                                                                     >  1:3847095/1‑93 (MQ=255)
         aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAtata                                                                                  <  1:332830/93‑1 (MQ=255)
         aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAtata                                                                                  <  2:6323320/93‑1 (MQ=255)
         aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAtata                                                                                  >  1:628490/1‑93 (MQ=255)
         aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAtata                                                                                  >  1:3114776/1‑93 (MQ=255)
          tGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAg                                                                                 >  2:5930795/1‑93 (MQ=255)
          tGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCAGTTGCCCACTGGCAATATAg                                                                                 >  1:5180583/1‑93 (MQ=255)
           gCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGt                                                                                <  2:2696364/93‑1 (MQ=255)
            cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTc                                                                               <  2:3947060/93‑1 (MQ=255)
               tGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGc                                                                            >  2:5035536/1‑93 (MQ=255)
               tGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGc                                                                            <  1:1865103/93‑1 (MQ=255)
                 tttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCa                                                                          <  2:5069379/93‑1 (MQ=255)
                 tttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCa                                                                          >  1:1154228/1‑93 (MQ=255)
                 tttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCa                                                                          >  2:2728238/1‑93 (MQ=255)
                   tCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCaga                                                                        <  1:1449571/93‑1 (MQ=255)
                    cAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCagag                                                                       >  1:110832/1‑93 (MQ=255)
                     aaaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGc                                                                      <  1:2357759/93‑1 (MQ=255)
                      aaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCa                                                                     >  1:1181796/1‑93 (MQ=255)
                      aaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCa                                                                     <  1:1604889/93‑1 (MQ=255)
                         tGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAaca                                                                  <  2:1450527/93‑1 (MQ=255)
                           cGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAAcaca                                                                <  2:19157/93‑1 (MQ=255)
                            gTATCCTTGAGATGAAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACaaaa                                                               >  2:1779762/1‑93 (MQ=255)
                            gTACCCTTGAGATGCATCTACTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACaa                                                               <  2:5884631/93‑1 (MQ=255)
                             tATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAg                                                              >  2:1706011/1‑93 (MQ=255)
                             tATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAg                                                              >  1:4496425/1‑93 (MQ=255)
                              aTCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGc                                                             >  2:295984/1‑93 (MQ=255)
                              aTCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGc                                                             <  2:5281219/93‑1 (MQ=255)
                               tCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCg                                                            >  2:6585221/1‑93 (MQ=255)
                               tCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCg                                                            >  2:383264/1‑93 (MQ=255)
                                ccTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGt                                                           >  1:3658295/1‑93 (MQ=255)
                                  ttGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCa                                                         >  2:6506368/1‑93 (MQ=255)
                                  ttGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCa                                                         <  1:5326208/93‑1 (MQ=255)
                                   tGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAt                                                        >  2:3697656/1‑93 (MQ=255)
                                     agaTGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAttt                                                      <  2:5089986/93‑1 (MQ=255)
                                     agaTGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAttt                                                      <  2:3985636/93‑1 (MQ=255)
                                      gaTGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTc                                                     >  2:5997173/1‑93 (MQ=255)
                                      gaTGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTc                                                     >  2:1249956/1‑93 (MQ=255)
                                          cAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGa                                                 <  1:3405195/93‑1 (MQ=255)
                                           aGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGaa                                                <  1:1249267/93‑1 (MQ=255)
                                            gCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAt                                               >  1:5644266/1‑93 (MQ=255)
                                            gCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAt                                               >  1:5156440/1‑93 (MQ=255)
                                            gCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAt                                               >  2:5377063/1‑93 (MQ=255)
                                              ttCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATcc                                             >  1:1146002/1‑93 (MQ=255)
                                              ttCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATcc                                             >  2:1555108/1‑93 (MQ=255)
                                              ttCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATcc                                             >  2:3756635/1‑93 (MQ=255)
                                              ttCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATcc                                             <  2:5260039/93‑1 (MQ=255)
                                                ctcGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCcac                                           <  2:448674/93‑1 (MQ=255)
                                                  cGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACaa                                         <  1:2956671/93‑1 (MQ=255)
                                                   ggCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAAt                                        >  2:1532009/1‑93 (MQ=255)
                                                   ggCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAAt                                        <  2:1640184/93‑1 (MQ=255)
                                                     cATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTa                                      >  2:4817485/1‑93 (MQ=255)
                                                     cATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTa                                      >  1:4738215/1‑93 (MQ=255)
                                                      aTAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTaa                                     >  2:331163/1‑93 (MQ=255)
                                                      aTAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTaa                                     >  2:1110196/1‑93 (MQ=255)
                                                      aTAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTaa                                     >  1:2071617/1‑93 (MQ=255)
                                                      aTAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTaa                                     >  1:4218390/1‑93 (MQ=255)
                                                      aTAGTTGTTCATCATTTCAACAAACTGCCGTTGCACACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTaa                                     >  1:6189435/1‑93 (MQ=255)
                                                       tAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTaaa                                    <  1:5809224/93‑1 (MQ=255)
                                                        aGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAAc                                   <  1:4613688/93‑1 (MQ=255)
                                                           tgttCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCAc                                <  2:2412379/93‑1 (MQ=255)
                                                            gttCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACg                               <  2:5149189/93‑1 (MQ=255)
                                                             ttCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACgg                              >  1:501569/1‑93 (MQ=255)
                                                             ttCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACgg                              <  1:81298/93‑1 (MQ=255)
                                                                atcatTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCtt                           >  2:842047/1‑93 (MQ=255)
                                                                atcatTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCtt                           >  1:3265722/1‑93 (MQ=255)
                                                                  catTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTaa                         <  1:26004/93‑1 (MQ=255)
                                                                   atTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTaaa                        <  2:3353460/93‑1 (MQ=255)
                                                                    tttCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAAt                       <  2:4240191/93‑1 (MQ=255)
                                                                      tCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCa                     >  1:726497/1‑93 (MQ=255)
                                                                       caacaaACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCAc                    <  1:2641004/93‑1 (MQ=255)
                                                                        aacaaACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACg                   >  1:4910832/1‑93 (MQ=255)
                                                                           aaaCTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGtgc                <  1:5562771/93‑3 (MQ=255)
                                                                           aaaCTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTAc                <  2:2895523/93‑1 (MQ=255)
                                                                             aCTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCg              <  2:6094357/93‑1 (MQ=255)
                                                                              cTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGa             >  1:6394459/1‑93 (MQ=255)
                                                                               tGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGAt            <  1:187531/93‑1 (MQ=255)
                                                                                gCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATa           <  2:3524834/93‑1 (MQ=255)
                                                                                   gTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATAcgc        >  2:4334455/1‑93 (MQ=255)
                                                                                    ttGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATAcgcg       >  1:4133134/1‑93 (MQ=255)
                                                                                    ttGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATAcgcg       >  2:5550867/1‑93 (MQ=255)
                                                                                    ttGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATAcgcg       >  1:6106263/1‑93 (MQ=255)
                                                                                     tGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGa      <  2:1122559/93‑1 (MQ=255)
                                                                                     tGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGa      >  1:5887266/1‑93 (MQ=255)
                                                                                     tGCCCACTGGCAATATAGTCAGCCAGAGAAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGa      <  1:4242369/93‑1 (MQ=255)
                                                                                      gCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGAt     >  1:2664923/1‑93 (MQ=255)
                                                                                      gCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGAt     <  2:4052716/93‑1 (MQ=255)
                                                                                       cccACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATc    <  1:90845/93‑1 (MQ=255)
                                                                                       cccACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATc    <  1:1689948/93‑1 (MQ=255)
                                                                                         cACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGc  >  1:3590918/1‑93 (MQ=255)
                                                                                            |                                                                                         
ATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGC  >  NC_000913/2080051‑2080232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCCTC  >  NC_000913/2080050‑2080236
                                                                                             |                                                                                             
CATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACT                                                                                               >  SRR4302547.4961952/1‑94 (MQ=60)
CATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGACCACT                                                                                               >  SRR4302547.2396728/1‑94 (MQ=60)
 ATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTG                                                                                              <  SRR4302547.6172419/94‑1 (MQ=60)
 ATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTG                                                                                              >  SRR4302547.5003657/1‑94 (MQ=60)
  TCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGG                                                                                             <  SRR4302547.682137/94‑1 (MQ=60)
  TCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGG                                                                                             <  SRR4302547.407163/94‑1 (MQ=60)
   CCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGC                                                                                            <  SRR4302547.4113004/94‑1 (MQ=60)
    CAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCA                                                                                           >  SRR4302547.4953767/1‑94 (MQ=60)
      GACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAT                                                                                         >  SRR4302547.3925417/1‑94 (MQ=60)
      GACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAT                                                                                         <  SRR4302547.3226624/94‑1 (MQ=60)
      GACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAAT                                                                                         >  SRR4302547.2036448/1‑94 (MQ=60)
       ACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATA                                                                                        <  SRR4302547.4050577/94‑1 (MQ=60)
       ACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATA                                                                                        >  SRR4302547.971509/1‑94 (MQ=60)
         CATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATA                                                                                      >  SRR4302547.3176508/1‑94 (MQ=60)
         CATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATA                                                                                      >  SRR4302547.6252905/1‑94 (MQ=60)
         CATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATA                                                                                      >  SRR4302547.640093/1‑94 (MQ=60)
          ATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAG                                                                                     <  SRR4302547.339018/94‑1 (MQ=60)
          ATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCAGTTGCCCACTGGCAATATAG                                                                                     >  SRR4302547.5287205/1‑94 (MQ=60)
            GCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTC                                                                                   >  SRR4302547.1907758/1‑94 (MQ=60)
             CACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCA                                                                                  >  SRR4302547.693210/1‑94 (MQ=60)
             CACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCA                                                                                  <  SRR4302547.5408508/94‑1 (MQ=60)
              ACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAG                                                                                 <  SRR4302547.553635/94‑1 (MQ=60)
              ACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAG                                                                                 <  SRR4302547.2673922/94‑1 (MQ=60)
               CTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGC                                                                                >  SRR4302547.526392/1‑94 (MQ=60)
                TGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCC                                                                               <  SRR4302547.1901932/94‑1 (MQ=60)
                 GTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCA                                                                              >  SRR4302547.1177581/1‑94 (MQ=60)
                    TCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAG                                                                           >  SRR4302547.112854/1‑94 (MQ=60)
                    TCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAG                                                                           <  SRR4302547.1478559/94‑1 (MQ=60)
                      AAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCA                                                                         >  SRR4302547.1205670/1‑94 (MQ=60)
                      AAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCA                                                                         <  SRR4302547.2405538/94‑1 (MQ=60)
                       AAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAA                                                                        <  SRR4302547.6413923/94‑1 (MQ=60)
                       AAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAA                                                                        <  SRR4302547.1636717/94‑1 (MQ=60)
                             GTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAG                                                                  >  SRR4302547.4589296/1‑94 (MQ=60)
                               ATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCG                                                                <  SRR4302547.3458007/94‑1 (MQ=60)
                                TCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAATCGT                                                               >  SRR4302547.5420611/1‑94 (MQ=60)
                                TCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGT                                                               >  SRR4302547.3732934/1‑94 (MQ=60)
                                TCATTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTGAAAAAACTGCCGTTGCCCACTGGCAATATAGTCAGTCAGAGCAACACAAGCGC                                                               >  SRR4302547.3206318/1‑94 (MQ=60)
                                 CCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTC                                                              <  SRR4302547.6476978/94‑1 (MQ=60)
                                 CCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTC                                                              >  SRR4302547.3936610/1‑94 (MQ=60)
                                 CCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTC                                                              <  SRR4302547.1164108/94‑1 (MQ=60)
                                 CCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTC                                                              >  SRR4302547.1706244/1‑94 (MQ=60)
                                   TTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAT                                                            <  SRR4302547.5435986/94‑1 (MQ=60)
                                   TTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAT                                                            <  SRR4302547.5045840/94‑1 (MQ=60)
                                      AGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTC                                                         >  SRR4302547.4878547/1‑94 (MQ=60)
                                          GCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGA                                                     >  SRR4302547.5717678/1‑94 (MQ=60)
                                           CAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAA                                                    <  SRR4302547.3474479/94‑1 (MQ=60)
                                            AGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAT                                                   <  SRR4302547.1274479/94‑1 (MQ=60)
                                            AGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAT                                                   >  SRR4302547.5262618/1‑94 (MQ=60)
                                            AGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAT                                                   >  SRR4302547.5760767/1‑94 (MQ=60)
                                            AGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAAT                                                   <  SRR4302547.4469638/94‑1 (MQ=60)
                                              CTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCC                                                 >  SRR4302547.1169172/1‑94 (MQ=60)
                                              CTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCC                                                 >  SRR4302547.12217/1‑94 (MQ=60)
                                               TTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCA                                                >  SRR4302547.1200458/1‑94 (MQ=60)
                                                   CGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAAT                                            <  SRR4302547.3015524/94‑1 (MQ=60)
                                                    CGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATT                                           <  SRR4302547.1508180/94‑1 (MQ=60)
                                                     GCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTA                                          >  SRR4302547.4836417/1‑94 (MQ=60)
                                                      CATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAA                                         >  SRR4302547.4304524/1‑94 (MQ=60)
                                                      CATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAA                                         >  SRR4302547.2112966/1‑94 (MQ=60)
                                                      CATAGTTGTTCATCATTTCAACAAACTGCCGTTGCACACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAA                                         >  SRR4302547.6317912/1‑94 (MQ=60)
                                                       ATAGTTGTTTATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAA                                        >  SRR4302547.3561163/1‑94 (MQ=60)
                                                       ATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAA                                        >  SRR4302547.2425204/1‑94 (MQ=60)
                                                        TAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAAC                                       <  SRR4302547.5929410/94‑1 (MQ=60)
                                                        TAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAAC                                       <  SRR4302547.3156366/94‑1 (MQ=60)
                                                         AGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACC                                      <  SRR4302547.4709197/94‑1 (MQ=60)
                                                           TTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCAC                                    <  SRR4302547.1775378/94‑1 (MQ=60)
                                                             GTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGG                                  <  SRR4302547.2567233/94‑1 (MQ=60)
                                                             GTTCATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGG                                  >  SRR4302547.510703/1‑94 (MQ=60)
                                                              TTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGC                                 <  SRR4302547.82816/94‑1 (MQ=60)
                                                                CATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTT                               >  SRR4302547.5882774/1‑94 (MQ=60)
                                                                CATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTT                               >  SRR4302547.3331052/1‑94 (MQ=60)
                                                                 ATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTA                              <  SRR4302547.2954234/94‑1 (MQ=60)
                                                                 ATCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTA                              >  SRR4302547.48067/1‑94 (MQ=60)
                                                                  TCATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAA                             <  SRR4302547.845014/94‑1 (MQ=60)
                                                                   CATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAA                            <  SRR4302547.4067391/94‑1 (MQ=60)
                                                                   CATTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAA                            <  SRR4302547.26482/94‑1 (MQ=60)
                                                                    ATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAAT                           >  SRR4302547.2103276/1‑94 (MQ=60)
                                                                     TTTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATC                          >  SRR4302547.2706144/1‑94 (MQ=60)
                                                                      TTCAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCA                         >  SRR4302547.740045/1‑94 (MQ=60)
                                                                        CAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACG                       >  SRR4302547.5012520/1‑94 (MQ=60)
                                                                        CAACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACG                       <  SRR4302547.2694160/94‑1 (MQ=60)
                                                                         AACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGT                      >  SRR4302547.784584/1‑94 (MQ=60)
                                                                         AACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGT                      >  SRR4302547.2008920/1‑94 (MQ=60)
                                                                         AACAAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGT                      >  SRR4302547.3811245/1‑94 (MQ=60)
                                                                            AAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTGCC                   <  SRR4302547.5677496/94‑1 (MQ=60)
                                                                            AAACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACC                   <  SRR4302547.3988025/94‑1 (MQ=60)
                                                                              ACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGA                 <  SRR4302547.5169602/94‑1 (MQ=60)
                                                                              ACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGA                 >  SRR4302547.4274897/1‑94 (MQ=60)
                                                                              ACTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGA                 >  SRR4302547.6527855/1‑94 (MQ=60)
                                                                               CTGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGAT                <  SRR4302547.4316369/94‑1 (MQ=60)
                                                                                TGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATA               >  SRR4302547.5966146/1‑94 (MQ=60)
                                                                                TGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATA               <  SRR4302547.2245015/94‑1 (MQ=60)
                                                                                TGCCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATA               <  SRR4302547.191066/94‑1 (MQ=60)
                                                                                TGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATA               <  SRR4302547.4126819/94‑1 (MQ=60)
                                                                                  CCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACG             <  SRR4302547.3369567/94‑1 (MQ=60)
                                                                                  CCGTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACG             <  SRR4302547.4622762/94‑1 (MQ=60)
                                                                                    GTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCG           >  SRR4302547.4217231/1‑94 (MQ=60)
                                                                                    GTTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCG           >  SRR4302547.6232930/1‑94 (MQ=60)
                                                                                    GTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCG           <  SRR4302547.1386010/94‑1 (MQ=60)
                                                                                     TTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGA          >  SRR4302547.6009149/1‑94 (MQ=60)
                                                                                     TTGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGA          >  SRR4302547.4987527/1‑94 (MQ=60)
                                                                                      TGCCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGAT         >  SRR4302547.2718474/1‑94 (MQ=60)
                                                                                      TGCCCACTGGCAATATAGTCAGCCAGAGAAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGAT         <  SRR4302547.4329127/94‑1 (MQ=60)
                                                                                       GCCCTCTGGCAATATAGTCAGCCAGAGCAACGCAAGCGTCATTTCCGGAAGCCACAATTAAACCGCGGCTTAAATCGCGTACCGTTACGCGATC        <  SRR4302547.3299719/94‑1 (MQ=60)
                                                                                        CCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCG       >  SRR4302547.6028463/1‑94 (MQ=60)
                                                                                        CCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCG       <  SRR4302547.1723194/94‑1 (MQ=60)
                                                                                        CCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCG       <  SRR4302547.92536/94‑1 (MQ=60)
                                                                                        CCCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCG       <  SRR4302547.3256430/94‑1 (MQ=60)
                                                                                         CCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGC      <  SRR4302547.3696797/94‑1 (MQ=60)
                                                                                         CCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGC      >  SRR4302547.3664174/1‑94 (MQ=60)
                                                                                         CCACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGC      >  SRR4302547.1176257/1‑94 (MQ=60)
                                                                                          CACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGGATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCC     >  SRR4302547.4603981/1‑94 (MQ=60)
                                                                                           ACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCC    <  SRR4302547.30795/94‑1 (MQ=60)
                                                                                           ACTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCC    >  SRR4302547.2833009/1‑94 (MQ=60)
                                                                                            CTGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCCT   >  SRR4302547.1376328/1‑94 (MQ=60)
                                                                                             TGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCCTC  <  SRR4302547.1760614/94‑1 (MQ=60)
                                                                                             TGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCCTC  <  SRR4302547.4860910/94‑1 (MQ=60)
                                                                                             |                                                                                             
CATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATCCACAATTAAACCACGGCTTAAATCACGTACCGATACGCGATCGCCCTC  >  NC_000913/2080050‑2080236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑