Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,080,595 A→G intergenic (‑20/‑183) dacD ← / → sbcB penicillin binding protein 6b/exonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,080,5950AG85.5% 615.5 / 90.2 234intergenic (‑20/‑183)dacD/sbcBpenicillin binding protein 6b/exonuclease I
Reads supporting (aligned to +/- strand):  ref base A (24/10);  new base G (107/93);  total (131/103)
Fisher's exact test for biased strand distribution p-value = 9.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

Only 100 of 237 total aligned reads displayed.

GCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTT  >  NC_000913/2080505‑2080687
                                                                                          |                                                                                            
gccgcAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGc                                                                                            >  1:786755/1‑93 (MQ=255)
 ccgcAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCt                                                                                           <  1:3012613/93‑1 (MQ=255)
   gcAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGt                                                                                         <  1:4105532/93‑1 (MQ=255)
   gcAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGt                                                                                         >  1:5972099/1‑93 (MQ=255)
     aaaaCCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTc                                                                                       >  2:4765776/1‑93 (MQ=255)
     aaaaCCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTc                                                                                       >  2:2210742/1‑93 (MQ=255)
     aaaaCCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTAAACAGCAAACCCTCAGGAGTTTCAAATGGCTGttt                                                                                       >  2:4505306/1‑92 (MQ=255)
      aaaCCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCt                                                                                      >  2:5974654/1‑93 (MQ=255)
      aaaCCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCt                                                                                      <  1:4714967/93‑1 (MQ=255)
       aaCCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCtt                                                                                     >  1:6246075/1‑93 (MQ=255)
        aCCAGACGACAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCttt                                                                                    >  1:3910458/1‑93 (MQ=255)
         ccAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCtttt                                                                                   <  1:3807751/93‑1 (MQ=255)
         ccAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCtttt                                                                                   >  2:5859909/1‑93 (MQ=255)
          cAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCttttt                                                                                  >  1:4304065/1‑93 (MQ=255)
          cAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCttttt                                                                                  >  2:1233224/1‑93 (MQ=255)
          cAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGGTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCttttt                                                                                  >  1:2341312/1‑93 (MQ=255)
           aGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTa                                                                                 <  2:3728315/93‑1 (MQ=255)
            gACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTAc                                                                                >  2:6181706/1‑93 (MQ=255)
            gACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTAc                                                                                <  1:3264473/93‑1 (MQ=255)
            gACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTAc                                                                                >  2:3607652/1‑93 (MQ=255)
             aCGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACg                                                                               <  1:3125283/93‑1 (MQ=255)
               gATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGa                                                                             >  1:1198242/1‑93 (MQ=255)
                 tAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTTTGGaaa                                                                           >  1:3532407/1‑93 (MQ=255)
                 tAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGaaa                                                                           <  2:3233247/93‑1 (MQ=255)
                 tAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGaaa                                                                           >  2:3159829/1‑93 (MQ=255)
                  aaGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAAt                                                                          <  1:5917482/93‑1 (MQ=255)
                   aGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTGTTTTTACGGAAATa                                                                         >  1:4624532/1‑93 (MQ=255)
                   aGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATa                                                                         >  1:4459626/1‑93 (MQ=255)
                   aGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATa                                                                         >  1:4984807/1‑93 (MQ=255)
                   aGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATa                                                                         >  2:2712962/1‑93 (MQ=255)
                      tAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACtt                                                                      <  1:6546185/93‑1 (MQ=255)
                        aaaaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTAt                                                                    <  2:3356146/93‑1 (MQ=255)
                         aaaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTAta                                                                   >  2:2272211/1‑92 (MQ=255)
                         aaaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATg                                                                   <  2:4174239/93‑1 (MQ=255)
                         aaaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATg                                                                   >  1:1465887/1‑93 (MQ=255)
                         aaaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATg                                                                   <  1:3705095/93‑1 (MQ=255)
                         aaaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATg                                                                   <  1:1804270/93‑1 (MQ=255)
                          aaCGAACAAAGAAGCAGCAATAATAAGACTGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGa                                                                  <  1:4358272/93‑1 (MQ=255)
                          aaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGa                                                                  <  2:3558311/93‑1 (MQ=255)
                          aaCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGa                                                                  <  1:24907/93‑1 (MQ=255)
                           aCGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGaa                                                                 <  1:2959154/93‑1 (MQ=255)
                            cGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAc                                                                <  2:1418346/93‑1 (MQ=255)
                            cGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAc                                                                >  2:1024904/1‑93 (MQ=255)
                            cGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAc                                                                >  1:5632212/1‑93 (MQ=255)
                             gAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAGTAGCTGTTCTTTTTACGGAAATACTTATGAAct                                                               >  1:2545737/1‑93 (MQ=255)
                              aaCAGAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAActg                                                              >  2:4932171/1‑93 (MQ=255)
                                cAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAActggc                                                            <  2:156764/93‑1 (MQ=255)
                                cAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAActggc                                                            <  2:2292016/93‑1 (MQ=255)
                                 aaaGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAActggct                                                           >  2:2028420/1‑93 (MQ=255)
                                  aagaagCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAActggctg                                                          <  2:6377455/93‑1 (MQ=255)
                                   agaagCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAActggctgg                                                         <  1:1162780/93‑1 (MQ=255)
                                    gaagCAGCAATAATAAGACGGCGTTTCAACAGCAAACCATCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGa                                                        <  1:5380390/93‑1 (MQ=255)
                                     aagCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGaa                                                       >  2:6122631/1‑93 (MQ=255)
                                       gcagcaATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATa                                                     >  2:1788129/1‑93 (MQ=255)
                                        cagcaATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATaa                                                    >  1:1259823/1‑93 (MQ=255)
                                        cagcaATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATaa                                                    >  2:5640404/1‑93 (MQ=255)
                                           caATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGt                                                 <  2:5305241/93‑1 (MQ=255)
                                             ataataAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGc                                               <  2:4441441/93‑1 (MQ=255)
                                              taataaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCa                                              <  2:5022985/93‑1 (MQ=255)
                                              taataaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCa                                              >  1:2048632/1‑93 (MQ=255)
                                              taataaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCa                                              >  2:3883345/1‑93 (MQ=255)
                                                ataaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAg                                            >  2:2909219/1‑93 (MQ=255)
                                                ataaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAg                                            >  1:3356381/1‑93 (MQ=255)
                                                ataaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAg                                            >  2:4561302/1‑93 (MQ=255)
                                                 taaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGa                                           >  1:3384350/1‑93 (MQ=255)
                                                  aaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGaa                                          >  2:2483332/1‑93 (MQ=255)
                                                  aaGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGaa                                          >  2:536684/1‑93 (MQ=255)
                                                    gACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGaaaa                                        <  2:2986005/93‑1 (MQ=255)
                                                    gACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGaaaa                                        >  1:665420/1‑93 (MQ=255)
                                                    gACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGaaaa                                        >  2:10001/1‑93 (MQ=255)
                                                     aCGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAt                                       <  1:5212680/93‑1 (MQ=255)
                                                      cGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAtg                                      <  1:2471205/93‑1 (MQ=255)
                                                       ggCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAtgt                                     >  2:724590/1‑93 (MQ=255)
                                                        gCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAtgtg                                    >  1:5843282/1‑93 (MQ=255)
                                                        gCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAtgtg                                    <  1:6479948/93‑1 (MQ=255)
                                                           tttCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACt                                 >  1:4145060/1‑93 (MQ=255)
                                                            ttCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTa                                >  1:3298426/1‑93 (MQ=255)
                                                              cAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTAcc                              >  2:1643397/1‑93 (MQ=255)
                                                                aCAGCAAACCCTCAGGAGTTTCGAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCct                            >  2:744534/1‑93 (MQ=255)
                                                                aCAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCct                            >  1:1923871/1‑93 (MQ=255)
                                                                aCAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCct                            <  2:1940538/93‑1 (MQ=255)
                                                                aCAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCct                            >  2:189757/1‑93 (MQ=255)
                                                                  aGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCctct                          <  2:1510583/93‑1 (MQ=255)
                                                                   gCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCtctc                         <  2:2573106/93‑1 (MQ=255)
                                                                   gCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCtctc                         <  2:5439283/93‑1 (MQ=255)
                                                                    cAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCa                        <  1:1100594/93‑1 (MQ=255)
                                                                     aaaCCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAt                       <  2:2854665/93‑1 (MQ=255)
                                                                       aCCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAttt                     >  2:4589848/1‑93 (MQ=255)
                                                                       aCCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAttt                     >  1:4031381/1‑93 (MQ=255)
                                                                        cccTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAtttt                    >  1:5559352/1‑93 (MQ=255)
                                                                         ccTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAttttt                   >  2:813932/1‑93 (MQ=255)
                                                                         ccTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAttttt                   >  1:217796/1‑93 (MQ=255)
                                                                          cTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTa                  <  1:1587212/93‑1 (MQ=255)
                                                                             aGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCt               >  2:541283/1‑93 (MQ=255)
                                                                               gAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGa             >  1:4522595/1‑93 (MQ=255)
                                                                                  tttCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACAt          <  1:5103849/93‑1 (MQ=255)
                                                                                  tttCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACAt          <  1:3955792/93‑1 (MQ=255)
                                                                                      aaaTGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATc      >  2:6284346/1‑93 (MQ=255)
                                                                                       aaTAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCt     <  2:2350301/93‑1 (MQ=255)
                                                                                          aGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGtt  <  2:6514739/93‑1 (MQ=255)
                                                                                          |                                                                                            
GCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTT  >  NC_000913/2080505‑2080687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGT  >  NC_000913/2080502‑2080686
                                                                                             |                                                                                           
TCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATT                                                                                             <  SRR4302547.2446202/94‑1 (MQ=60)
  CGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGC                                                                                           >  SRR4302547.801644/1‑94 (MQ=60)
   GCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCT                                                                                          >  SRR4302547.3351684/1‑94 (MQ=60)
    CCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTG                                                                                         <  SRR4302547.3203476/94‑1 (MQ=60)
    CCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTG                                                                                         <  SRR4302547.3072507/94‑1 (MQ=60)
     CGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGT                                                                                        <  SRR4302547.6323381/94‑1 (MQ=60)
     CGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGT                                                                                        >  SRR4302547.6095959/1‑94 (MQ=60)
     CGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGT                                                                                        <  SRR4302547.2175589/94‑1 (MQ=60)
      GCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTT                                                                                       <  SRR4302547.4188992/94‑1 (MQ=60)
      GCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTT                                                                                       >  SRR4302547.70137/1‑94 (MQ=60)
       CAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTC                                                                                      >  SRR4302547.2971301/1‑94 (MQ=60)
       CAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTC                                                                                      >  SRR4302547.566886/1‑94 (MQ=60)
        AAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCT                                                                                     <  SRR4302547.1165251/94‑1 (MQ=60)
         AAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTT                                                                                    <  SRR4302547.4812580/94‑1 (MQ=60)
         AAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTT                                                                                    >  SRR4302547.6375823/1‑94 (MQ=60)
          AACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTT                                                                                   >  SRR4302547.3452115/1‑94 (MQ=60)
          AACCAGACGACAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTT                                                                                   >  SRR4302547.3989980/1‑94 (MQ=60)
           ACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTT                                                                                  >  SRR4302547.563858/1‑94 (MQ=60)
            CCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTT                                                                                 <  SRR4302547.4000365/94‑1 (MQ=60)
            CCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTT                                                                                 >  SRR4302547.4392595/1‑94 (MQ=60)
            CCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTT                                                                                 <  SRR4302547.3885363/94‑1 (MQ=60)
            CCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGGTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTT                                                                                 >  SRR4302547.2388767/1‑94 (MQ=60)
              AGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTAC                                                                               <  SRR4302547.3611715/94‑1 (MQ=60)
              AGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTAC                                                                               <  SRR4302547.4156780/94‑1 (MQ=60)
               GACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACG                                                                              >  SRR4302547.2134909/1‑94 (MQ=60)
               GACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACG                                                                              <  SRR4302547.3489423/94‑1 (MQ=60)
               GACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACG                                                                              >  SRR4302547.4797304/1‑94 (MQ=60)
               GACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACG                                                                              <  SRR4302547.3329775/94‑1 (MQ=60)
               GACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACG                                                                              <  SRR4302547.4413698/94‑1 (MQ=60)
                ACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGG                                                                             <  SRR4302547.3187279/94‑1 (MQ=60)
                ACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGG                                                                             <  SRR4302547.404666/94‑1 (MQ=60)
                 CGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGA                                                                            >  SRR4302547.1222418/1‑94 (MQ=60)
                   ATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTTTGGAAA                                                                          >  SRR4302547.3604530/1‑94 (MQ=60)
                   ATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAA                                                                          >  SRR4302547.4967523/1‑94 (MQ=60)
                    TAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGGTTCAAATGGCTGTTCTTTTTACGGAAAA                                                                         >  SRR4302547.1420025/1‑94 (MQ=60)
                     AAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTGTTTTTACGGAAATA                                                                        >  SRR4302547.4720307/1‑94 (MQ=60)
                     AAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATA                                                                        >  SRR4302547.4551803/1‑94 (MQ=60)
                     AAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATA                                                                        <  SRR4302547.6040108/94‑1 (MQ=60)
                     AAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATA                                                                        >  SRR4302547.5087880/1‑94 (MQ=60)
                     AAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATA                                                                        >  SRR4302547.6041089/1‑94 (MQ=60)
                         TAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTA                                                                    <  SRR4302547.6682428/94‑1 (MQ=60)
                           AAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATG                                                                  >  SRR4302547.1495173/1‑94 (MQ=60)
                           AAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATG                                                                  >  SRR4302547.6643746/1‑94 (MQ=60)
                            AAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGA                                                                 <  SRR4302547.1839942/94‑1 (MQ=60)
                            AAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGA                                                                 <  SRR4302547.3780729/94‑1 (MQ=60)
                             AACGAACAAAGAAGCAGCAATAATAAGACTGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAA                                                                <  SRR4302547.4447789/94‑1 (MQ=60)
                             AACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAA                                                                <  SRR4302547.25368/94‑1 (MQ=60)
                              ACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAC                                                               <  SRR4302547.4406674/94‑1 (MQ=60)
                              ACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAC                                                               >  SRR4302547.5748449/1‑94 (MQ=60)
                              ACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAC                                                               >  SRR4302547.94085/1‑94 (MQ=60)
                              ACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAC                                                               >  SRR4302547.3065086/1‑94 (MQ=60)
                              ACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAAC                                                               <  SRR4302547.3018050/94‑1 (MQ=60)
                               CGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAGTAGCTGTTCTTTTTACGGAAATACTTATGAACT                                                              >  SRR4302547.2597149/1‑94 (MQ=60)
                               CGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACT                                                              <  SRR4302547.3262918/94‑1 (MQ=60)
                               CGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACT                                                              >  SRR4302547.5188192/1‑94 (MQ=60)
                                GAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTG                                                             >  SRR4302547.4374834/1‑94 (MQ=60)
                                 AACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGG                                                            >  SRR4302547.5773420/1‑94 (MQ=60)
                                 AACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGG                                                            >  SRR4302547.4717554/1‑94 (MQ=60)
                                 AACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGG                                                            >  SRR4302547.1962993/1‑94 (MQ=60)
                                   CAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCT                                                          <  SRR4302547.4195064/94‑1 (MQ=60)
                                    AAAGAAGCAGCAATAATAGGACGGCGTATCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGCACTGGCTG                                                         <  SRR4302547.2516249/94‑1 (MQ=60)
                                    AAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTG                                                         >  SRR4302547.3729545/1‑94 (MQ=60)
                                     AAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGG                                                        >  SRR4302547.4609684/1‑94 (MQ=60)
                                      AGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGA                                                       <  SRR4302547.1186304/94‑1 (MQ=60)
                                      AGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGA                                                       <  SRR4302547.6652912/94‑1 (MQ=60)
                                       GAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAA                                                      >  SRR4302547.1015769/1‑94 (MQ=60)
                                       GAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCATCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAA                                                      <  SRR4302547.5491474/94‑1 (MQ=60)
                                         AGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATA                                                    <  SRR4302547.1194708/94‑1 (MQ=60)
                                          GCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAA                                                   >  SRR4302547.1285210/1‑94 (MQ=60)
                                             GCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGT                                                <  SRR4302547.6159171/94‑1 (MQ=60)
                                              CAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTG                                               >  SRR4302547.1685302/1‑94 (MQ=60)
                                              CAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTG                                               >  SRR4302547.3174767/1‑94 (MQ=60)
                                                ATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCA                                             >  SRR4302547.2089489/1‑94 (MQ=60)
                                                 TAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGCGCAA                                            >  SRR4302547.5199358/1‑94 (MQ=60)
                                                  AATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAG                                           >  SRR4302547.3424463/1‑94 (MQ=60)
                                                   ATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGA                                          <  SRR4302547.4459552/94‑1 (MQ=60)
                                                   ATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGA                                          >  SRR4302547.3453152/1‑94 (MQ=60)
                                                     AAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAA                                        <  SRR4302547.2425120/94‑1 (MQ=60)
                                                      AGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAA                                       >  SRR4302547.677678/1‑94 (MQ=60)
                                                       GACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAT                                      >  SRR4302547.2532778/1‑94 (MQ=60)
                                                        ACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATG                                     <  SRR4302547.5319976/94‑1 (MQ=60)
                                                         CGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGT                                    <  SRR4302547.2521156/94‑1 (MQ=60)
                                                         CGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGT                                    >  SRR4302547.3400423/1‑94 (MQ=60)
                                                          GGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTG                                   <  SRR4302547.3978951/94‑1 (MQ=60)
                                                          GGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTG                                   >  SRR4302547.5964166/1‑94 (MQ=60)
                                                          GGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTG                                   >  SRR4302547.1512808/1‑94 (MQ=60)
                                                           GCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGA                                  >  SRR4302547.1872045/1‑94 (MQ=60)
                                                           GCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGA                                  <  SRR4302547.6614861/94‑1 (MQ=60)
                                                            CGTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGAC                                 <  SRR4302547.674473/94‑1 (MQ=60)
                                                             GTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACT                                >  SRR4302547.4229434/1‑94 (MQ=60)
                                                             GTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACT                                >  SRR4302547.1233280/1‑94 (MQ=60)
                                                             GTTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACT                                >  SRR4302547.5443566/1‑94 (MQ=60)
                                                              TTTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTA                               >  SRR4302547.3364800/1‑94 (MQ=60)
                                                               TTCAACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAAAGGGACTAC                              >  SRR4302547.2569660/1‑94 (MQ=60)
                                                               TTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTAC                              >  SRR4302547.2763930/1‑94 (MQ=60)
                                                                  AACAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCT                           >  SRR4302547.1961976/1‑94 (MQ=60)
                                                                    CAGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCT                         <  SRR4302547.4007685/94‑1 (MQ=60)
                                                                     AGCAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTC                        >  SRR4302547.1242144/1‑94 (MQ=60)
                                                                       CAAACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCAT                      <  SRR4302547.1122710/94‑1 (MQ=60)
                                                                         AACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTT                    >  SRR4302547.4113260/1‑94 (MQ=60)
                                                                         AACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTT                    >  SRR4302547.529524/1‑94 (MQ=60)
                                                                         AACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTT                    >  SRR4302547.2288110/1‑94 (MQ=60)
                                                                          ACCCTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTT                   >  SRR4302547.5674022/1‑94 (MQ=60)
                                                                           CCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTT                  >  SRR4302547.4646410/1‑94 (MQ=60)
                                                                           CCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTT                  >  SRR4302547.221902/1‑94 (MQ=60)
                                                                             CTCAGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTAT                <  SRR4302547.1618761/94‑1 (MQ=60)
                                                                                AGGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTG             >  SRR4302547.3382308/1‑94 (MQ=60)
                                                                                 GGAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGA            >  SRR4302547.4616063/1‑94 (MQ=60)
                                                                                  GAGTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGAC           >  SRR4302547.1678766/1‑94 (MQ=60)
                                                                                    GTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACAT         <  SRR4302547.383762/94‑1 (MQ=60)
                                                                                    GTTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACAT         <  SRR4302547.6023924/94‑1 (MQ=60)
                                                                                     TTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATG        <  SRR4302547.4215338/94‑1 (MQ=60)
                                                                                     TTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATG        <  SRR4302547.4036261/94‑1 (MQ=60)
                                                                                     TTTCAAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATG        <  SRR4302547.5209049/94‑1 (MQ=60)
                                                                                         AAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCT    <  SRR4302547.5939527/94‑1 (MQ=60)
                                                                                         AAATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCT    >  SRR4302547.5487294/1‑94 (MQ=60)
                                                                                          AATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTG   >  SRR4302547.6380435/1‑94 (MQ=60)
                                                                                           ATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGT  >  SRR4302547.3376243/1‑94 (MQ=60)
                                                                                           ATGGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGT  >  SRR4302547.4365542/1‑94 (MQ=60)
                                                                                             |                                                                                           
TCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGT  >  NC_000913/2080502‑2080686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑