Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,235,117 C→T A219A (GCG→GCA)  mglC ← MglC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,1170CT87.1% 689.0 / 68.9 233A219A (GCG→GCA) mglCMglC
Reads supporting (aligned to +/- strand):  ref base C (13/17);  new base T (103/100);  total (116/117)
Fisher's exact test for biased strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 245 total aligned reads displayed.

GCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGC  >  NC_000913/2235025‑2235206
                                                                                            |                                                                                         
gCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGcc                                                                                           >  1:3687529/1‑93 (MQ=255)
  ttCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTtgctgc                                                                                         >  2:6155303/1‑93 (MQ=255)
  ttCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTtgctgc                                                                                         <  1:3985914/93‑1 (MQ=255)
   tCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTgctgct                                                                                        >  1:1901471/1‑93 (MQ=255)
   tCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTgctgct                                                                                        <  2:1435086/93‑1 (MQ=255)
    cTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCtt                                                                                       <  1:5205968/93‑1 (MQ=255)
    cTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCtt                                                                                       >  1:785868/1‑93 (MQ=255)
     tAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATAGTTTTGCTGCTTc                                                                                      <  2:4524411/93‑1 (MQ=255)
     tAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTc                                                                                      >  1:2114230/1‑93 (MQ=255)
     tAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTc                                                                                      <  2:5017383/93‑1 (MQ=255)
     tAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTc                                                                                      <  2:3603740/93‑1 (MQ=255)
      aaCATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTcc                                                                                     <  2:1907415/93‑1 (MQ=255)
       aCATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATTCTTTTGCTGCTTCCg                                                                                    <  2:2816723/93‑1 (MQ=255)
       aCATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCg                                                                                    <  2:3083915/93‑1 (MQ=255)
       aCATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCg                                                                                    >  1:70913/1‑93 (MQ=255)
        cATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCgg                                                                                   >  1:4500531/1‑93 (MQ=255)
          tCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGt                                                                                 <  1:351002/93‑1 (MQ=255)
           cccGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGtt                                                                                <  2:4297961/93‑1 (MQ=255)
             cGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAc                                                                              >  1:6632396/1‑93 (MQ=255)
             cGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAc                                                                              >  1:5625641/1‑93 (MQ=255)
             cGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAc                                                                              >  1:3027948/1‑93 (MQ=255)
              gCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAcc                                                                             <  1:4924094/93‑1 (MQ=255)
               ccAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCg                                                                            >  1:1037674/1‑93 (MQ=255)
               ccAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCg                                                                            <  2:4328372/93‑1 (MQ=255)
                cAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGc                                                                           >  2:4649944/1‑93 (MQ=255)
                cAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGc                                                                           <  2:743048/93‑1 (MQ=255)
                cAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGc                                                                           >  2:6321127/1‑93 (MQ=255)
                 aaaGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGcc                                                                          >  2:6334222/1‑93 (MQ=255)
                 aaaGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGcc                                                                          <  2:1405160/93‑1 (MQ=255)
                  aaGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCa                                                                         <  2:2247990/93‑1 (MQ=255)
                   aGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGccca                                                                        >  2:3695131/1‑91 (MQ=255)
                   aGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCaa                                                                        <  1:4823721/93‑1 (MQ=255)
                    ggCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAAt                                                                       >  1:5226451/1‑93 (MQ=255)
                     gCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATg                                                                      <  2:1797939/93‑1 (MQ=255)
                     gCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATg                                                                      <  2:5250806/93‑1 (MQ=255)
                       aTAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGc                                                                    <  1:1482601/93‑1 (MQ=255)
                         aGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCaa                                                                  >  1:3934911/1‑93 (MQ=255)
                         aGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCaa                                                                  >  2:1193574/1‑93 (MQ=255)
                         aGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCAAATGGCaa                                                                  >  2:2551049/1‑93 (MQ=255)
                            acacGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCaaaaa                                                               <  1:1724962/93‑1 (MQ=255)
                             cacGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAAt                                                              >  2:4761701/1‑93 (MQ=255)
                               cGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGt                                                            <  1:6018413/93‑1 (MQ=255)
                                gCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGtt                                                           <  2:2161313/93‑1 (MQ=255)
                                 ccAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTc                                                          <  1:4255528/93‑1 (MQ=255)
                                  cAGACAACTCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCt                                                         >  1:6466921/1‑93 (MQ=255)
                                  cAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCt                                                         >  1:5843928/1‑93 (MQ=255)
                                  cAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCt                                                         >  2:2433251/1‑93 (MQ=255)
                                  cAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCt                                                         >  2:5525100/1‑93 (MQ=255)
                                    gACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTa                                                       >  2:318539/1‑93 (MQ=255)
                                    gACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTa                                                       <  2:1614994/93‑1 (MQ=255)
                                     aCAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTAc                                                      <  2:5855776/93‑1 (MQ=255)
                                        aCGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGa                                                   <  1:1227276/93‑1 (MQ=255)
                                        aCGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGa                                                   <  1:1132555/93‑1 (MQ=255)
                                         cgcgTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGaa                                                  <  2:5579730/93‑1 (MQ=255)
                                          gcgTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAg                                                 >  1:6170810/1‑93 (MQ=255)
                                           cgTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGc                                                <  2:3331261/93‑1 (MQ=255)
                                           cgTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGc                                                <  2:5618296/93‑1 (MQ=255)
                                              aGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCggg                                             <  2:2693683/93‑1 (MQ=255)
                                               gATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGt                                            >  1:3861315/1‑93 (MQ=255)
                                               gATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCCGGt                                            >  1:2775193/1‑93 (MQ=255)
                                               gATCATCAGCAGGGTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCGTACCGAAGCGGGt                                            >  2:3791121/1‑93 (MQ=255)
                                               aatcatcAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGt                                            <  1:3060786/92‑1 (MQ=255)
                                                atcatcAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGtt                                           <  2:3358335/93‑1 (MQ=255)
                                                 tcatcaGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGttt                                          <  2:3531114/93‑1 (MQ=255)
                                                 tcatcaGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGttt                                          <  2:805363/93‑1 (MQ=255)
                                                 tcatcaGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGttt                                          <  1:2087014/93‑1 (MQ=255)
                                                   atcaGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTg                                        >  1:3325534/1‑93 (MQ=255)
                                                   atcaGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTg                                        <  2:4188005/93‑1 (MQ=255)
                                                     cagcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGtt                                      >  2:4060962/1‑93 (MQ=255)
                                                      agcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTc                                     >  1:3605919/1‑93 (MQ=255)
                                                      agcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTc                                     <  2:684948/93‑1 (MQ=255)
                                                      agcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTc                                     <  2:2978951/93‑1 (MQ=255)
                                                       gcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTcc                                    >  1:5938002/1‑93 (MQ=255)
                                                       gcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTcc                                    <  1:6351600/93‑1 (MQ=255)
                                                       gcagGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTcc                                    >  1:1538262/1‑93 (MQ=255)
                                                         agGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCcac                                  <  1:1354768/93‑1 (MQ=255)
                                                           gTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCacaa                                >  1:2794923/1‑93 (MQ=255)
                                                           gTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTCCCacaa                                <  1:5521530/93‑1 (MQ=255)
                                                                   cGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGa                        <  1:2546604/93‑1 (MQ=255)
                                                                   cGACGTTCACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGCTCTTACCGCAGCGGGTTTTGTTCCACAACACCCAGa                        >  1:1005878/1‑93 (MQ=255)
                                                                    gACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGAc                       >  1:6160091/1‑93 (MQ=255)
                                                                    gACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGAc                       >  2:2062159/1‑93 (MQ=255)
                                                                      cGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGt                                            >  2:5074774/1‑70 (MQ=255)
                                                                      cGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGt                                            <  1:5074774/70‑1 (MQ=255)
                                                                      cGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGa                     <  1:1786364/93‑1 (MQ=255)
                                                                        ttGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAAc                   <  2:4436646/93‑1 (MQ=255)
                                                                        ttGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAAc                   >  2:5497761/1‑93 (MQ=255)
                                                                          gACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGc                 <  1:455700/93‑1 (MQ=255)
                                                                           acacCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGcc                >  1:1581471/1‑93 (MQ=255)
                                                                           acacCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGcc                >  2:6575119/1‑93 (MQ=255)
                                                                            cacCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCa               >  2:4826140/1‑93 (MQ=255)
                                                                            cacCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCa               <  1:1438866/93‑1 (MQ=255)
                                                                             acCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCAc              >  2:2318771/1‑93 (MQ=255)
                                                                              ccAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCAcc             >  1:2373892/1‑93 (MQ=255)
                                                                               cAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCg            >  1:4329761/1‑93 (MQ=255)
                                                                               cAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGACCGCCACCg            >  1:5811357/1‑93 (MQ=255)
                                                                                aGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGc           >  1:4702010/1‑93 (MQ=255)
                                                                                    tcTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATc       <  1:3875496/92‑1 (MQ=255)
                                                                                      ttttGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCaa     <  1:3363391/93‑1 (MQ=255)
                                                                                         tGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAcgc  >  2:1010222/1‑93 (MQ=255)
                                                                                            |                                                                                         
GCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGC  >  NC_000913/2235025‑2235206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTA  >  NC_000913/2235024‑2235209
                                                                                             |                                                                                            
GGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCT                                                                                              <  SRR4302547.2832503/94‑1 (MQ=60)
GGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCT                                                                                              >  SRR4302547.5434377/1‑94 (MQ=60)
GGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCC                                                                                              >  SRR4302547.3762806/1‑94 (MQ=60)
GGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCC                                                                                              >  SRR4302547.2029303/1‑94 (MQ=60)
 GCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTG                                                                                             <  SRR4302547.4625276/94‑1 (MQ=60)
 GCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTG                                                                                             >  SRR4302547.6479515/1‑94 (MQ=60)
 GCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCG                                                                                             <  SRR4302547.2929123/94‑1 (MQ=60)
   TTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCT                                                                                           <  SRR4302547.901228/94‑1 (MQ=60)
   TTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCT                                                                                           <  SRR4302547.4066959/94‑1 (MQ=60)
   TTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCT                                                                                           >  SRR4302547.1939088/1‑94 (MQ=60)
   TTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCT                                                                                           >  SRR4302547.2172860/1‑94 (MQ=60)
    TCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTT                                                                                          >  SRR4302547.800737/1‑94 (MQ=60)
     CTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTC                                                                                         >  SRR4302547.2842559/1‑94 (MQ=60)
     CTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTC                                                                                         >  SRR4302547.2156353/1‑94 (MQ=60)
     CTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTC                                                                                         <  SRR4302547.5313093/94‑1 (MQ=60)
      TAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCC                                                                                        >  SRR4302547.5265682/1‑94 (MQ=60)
      TAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCC                                                                                        <  SRR4302547.1979220/94‑1 (MQ=60)
       AACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCG                                                                                       <  SRR4302547.2557210/94‑1 (MQ=60)
       AACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCG                                                                                       >  SRR4302547.72242/1‑94 (MQ=60)
       AACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCG                                                                                       >  SRR4302547.1156887/1‑94 (MQ=60)
        ACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGG                                                                                      >  SRR4302547.4593492/1‑94 (MQ=60)
          CTCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGT                                                                                    <  SRR4302547.4127589/94‑1 (MQ=60)
          ATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGT                                                                                    >  SRR4302547.1349530/1‑94 (MQ=60)
           TCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTT                                                                                   <  SRR4302547.637825/94‑1 (MQ=60)
           TCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTT                                                                                   <  SRR4302547.357520/94‑1 (MQ=60)
            CCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTA                                                                                  <  SRR4302547.3100716/94‑1 (MQ=60)
             CCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAC                                                                                 >  SRR4302547.3088156/1‑94 (MQ=60)
             CCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAC                                                                                 >  SRR4302547.538826/1‑94 (MQ=60)
             CCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAC                                                                                 >  SRR4302547.5741735/1‑94 (MQ=60)
             ACGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTAC                                                                                 >  SRR4302547.6770458/1‑94 (MQ=60)
              CGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACC                                                                                <  SRR4302547.3825300/94‑1 (MQ=60)
              CGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACC                                                                                <  SRR4302547.4166017/94‑1 (MQ=60)
               GCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCG                                                                               <  SRR4302547.5026091/94‑1 (MQ=60)
               GCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCG                                                                               >  SRR4302547.1058117/1‑94 (MQ=60)
               GCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCG                                                                               >  SRR4302547.3590168/1‑94 (MQ=60)
               GCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCG                                                                               >  SRR4302547.6763030/1‑94 (MQ=60)
               GCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCG                                                                               >  SRR4302547.186462/1‑94 (MQ=60)
               GCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCG                                                                               <  SRR4302547.5326089/94‑1 (MQ=60)
                CCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGC                                                                              <  SRR4302547.2362429/94‑1 (MQ=60)
                 CAAAGGCATAAAACACTCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCC                                                                             <  SRR4302547.1551757/94‑1 (MQ=60)
                  AAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCA                                                                            >  SRR4302547.676084/1‑94 (MQ=60)
                   AAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAA                                                                           >  SRR4302547.1812985/1‑94 (MQ=60)
                    AGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAAT                                                                          <  SRR4302547.4923699/94‑1 (MQ=60)
                    AGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAAT                                                                          >  SRR4302547.5334031/1‑94 (MQ=60)
                    AGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAAT                                                                          <  SRR4302547.3152901/94‑1 (MQ=60)
                     GGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATG                                                                         <  SRR4302547.3653816/94‑1 (MQ=60)
                      GCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGG                                                                        >  SRR4302547.155850/1‑94 (MQ=60)
                       CATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGC                                                                       <  SRR4302547.667378/94‑1 (MQ=60)
                        ATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCA                                                                      <  SRR4302547.1512189/94‑1 (MQ=60)
                         TAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAA                                                                     >  SRR4302547.4014930/1‑94 (MQ=60)
                         TAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAA                                                                     >  SRR4302547.1071078/1‑94 (MQ=60)
                            AACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAA                                                                  <  SRR4302547.6103135/94‑1 (MQ=60)
                             ACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAAT                                                                 <  SRR4302547.1758867/94‑1 (MQ=60)
                              CACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATG                                                                >  SRR4302547.4004218/1‑94 (MQ=60)
                              CACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATG                                                                <  SRR4302547.5124761/94‑1 (MQ=60)
                              CACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATG                                                                >  SRR4302547.3417012/1‑94 (MQ=60)
                               ACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGT                                                               >  SRR4302547.4597995/1‑94 (MQ=60)
                               ACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGT                                                               >  SRR4302547.3280914/1‑94 (MQ=60)
                                CGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTT                                                              <  SRR4302547.6143322/94‑1 (MQ=60)
                                CGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTT                                                              >  SRR4302547.862496/1‑94 (MQ=60)
                                 GCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTC                                                             >  SRR4302547.4621483/1‑94 (MQ=60)
                                  CCAGACAACTCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCT                                                            >  SRR4302547.6601577/1‑94 (MQ=60)
                                  CCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCT                                                            <  SRR4302547.4342605/94‑1 (MQ=60)
                                  CCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCT                                                            >  SRR4302547.5964821/1‑94 (MQ=60)
                                   CAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTT                                                           >  SRR4302547.5671276/1‑94 (MQ=60)
                                    AGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTA                                                          <  SRR4302547.4469348/94‑1 (MQ=60)
                                     GACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTAC                                                         <  SRR4302547.587592/94‑1 (MQ=60)
                                      ACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACC                                                        <  SRR4302547.3944569/94‑1 (MQ=60)
                                       CAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCG                                                       <  SRR4302547.1055840/94‑1 (MQ=60)
                                         ACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAA                                                     <  SRR4302547.1252041/94‑1 (MQ=60)
                                         ACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAA                                                     <  SRR4302547.1155411/94‑1 (MQ=60)
                                          CGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAG                                                    >  SRR4302547.1394825/1‑94 (MQ=60)
                                          CGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAG                                                    >  SRR4302547.6298906/1‑94 (MQ=60)
                                            CGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCG                                                  >  SRR4302547.6165865/1‑94 (MQ=60)
                                            CGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAACTGTTCTTACCGAAGCG                                                  >  SRR4302547.3970067/1‑94 (MQ=60)
                                             GTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGG                                                 >  SRR4302547.3010026/1‑94 (MQ=60)
                                             GTAGATCATCAGCAGATTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGG                                                 >  SRR4302547.4878452/1‑94 (MQ=60)
                                              TAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATCGTTTGGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTAGCGAAGCGGA                                                <  SRR4302547.1972564/94‑1 (MQ=60)
                                              TAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGG                                                <  SRR4302547.381506/94‑1 (MQ=60)
                                               AGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGT                                               <  SRR4302547.5090708/94‑1 (MQ=60)
                                               AGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGT                                               >  SRR4302547.2695181/1‑94 (MQ=60)
                                               AGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGT                                               >  SRR4302547.3939895/1‑94 (MQ=60)
                                               AGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCCGGT                                               >  SRR4302547.2830783/1‑94 (MQ=60)
                                                acgctcttccgatcttgacattACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTT                                              <  SRR4302547.5179469/72‑1 (MQ=60)
                                                GATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTT                                              <  SRR4302547.6295085/94‑1 (MQ=60)
                                                AATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTT                                              <  SRR4302547.3121669/94‑1 (MQ=60)
                                                 ATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTT                                             <  SRR4302547.4037108/94‑1 (MQ=60)
                                                  TCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTT                                            <  SRR4302547.4955207/94‑1 (MQ=60)
                                                  TCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTT                                            <  SRR4302547.2128636/94‑1 (MQ=60)
                                                   CATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTG                                           <  SRR4302547.2801005/94‑1 (MQ=60)
                                                   CATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTG                                           >  SRR4302547.3392729/1‑94 (MQ=60)
                                                     TCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTT                                         <  SRR4302547.2129793/94‑1 (MQ=60)
                                                      CAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTC                                        >  SRR4302547.3679458/1‑94 (MQ=60)
                                                       AGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCC                                       >  SRR4302547.6061131/1‑94 (MQ=60)
                                                       AGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCC                                       >  SRR4302547.1568887/1‑94 (MQ=60)
                                                        GCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCA                                      <  SRR4302547.6483956/94‑1 (MQ=60)
                                                         CAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCAC                                     >  SRR4302547.6276425/1‑94 (MQ=60)
                                                          AGGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACA                                    <  SRR4302547.1381961/94‑1 (MQ=60)
                                                           GGTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAA                                   >  SRR4302547.2850876/1‑94 (MQ=60)
                                                           GGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAA                                   >  SRR4302547.3457976/1‑94 (MQ=60)
                                                            GTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAAC                                  >  SRR4302547.773280/1‑94 (MQ=60)
                                                            GTTCAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTCCCACAAC                                  <  SRR4302547.5635507/94‑1 (MQ=60)
                                                             TGCAGGCCGACGTTTACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACA                                 <  SRR4302547.1735885/94‑1 (MQ=60)
                                                              TCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACAC                                <  SRR4302547.6509790/94‑1 (MQ=60)
                                                               CAGGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGCTCCACAACACC                               >  SRR4302547.1599286/1‑94 (MQ=60)
                                                                 GGCCGACGTTGACACCAGATACTTTTGGTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA                             <  SRR4302547.6421750/94‑1 (MQ=60)
                                                                 GGCCGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCA                             >  SRR4302547.1419718/1‑94 (MQ=60)
                                                                   CCGACGTTCACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGCTCTTACCGCAGCGGGTTTTGTTCCACAACACCCAGA                           >  SRR4302547.1025512/1‑94 (MQ=60)
                                                                    CGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGAC                          <  SRR4302547.2598030/94‑1 (MQ=60)
                                                                    CGACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGAC                          >  SRR4302547.6287932/1‑94 (MQ=60)
                                                                     GACGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACG                         >  SRR4302547.4416253/1‑94 (MQ=60)
                                                                       CGTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAA                       <  SRR4302547.1821739/94‑1 (MQ=60)
                                                                        GTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAAC                      <  SRR4302547.6036323/94‑1 (MQ=60)
                                                                        GTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAAC                      <  SRR4302547.5324822/94‑1 (MQ=60)
                                                                        GTTGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAAC                      <  SRR4302547.1477343/94‑1 (MQ=60)
                                                                          TGACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGC                    >  SRR4302547.4749724/1‑94 (MQ=60)
                                                                           GACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCC                   >  SRR4302547.1612941/1‑94 (MQ=60)
                                                                           GACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCC                   <  SRR4302547.464141/94‑1 (MQ=60)
                                                                            ACACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCA                  >  SRR4302547.1620589/1‑94 (MQ=60)
                                                                             CACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCAC                 <  SRR4302547.1467640/94‑1 (MQ=60)
                                                                              ACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCGGACGAACGCCACC                >  SRR4302547.368669/1‑94 (MQ=60)
                                                                              ACCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACC                >  SRR4302547.2421995/1‑94 (MQ=60)
                                                                               CCAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCG               >  SRR4302547.4418852/1‑94 (MQ=60)
                                                                               CCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGACCGCCACCG               >  SRR4302547.5931592/1‑94 (MQ=60)
                                                                                CAGATACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGC              >  SRR4302547.4799394/1‑94 (MQ=60)
                                                                                    TACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATC          <  SRR4302547.1186314/94‑1 (MQ=60)
                                                                                     TCTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCA         <  SRR4302547.3954351/94‑1 (MQ=60)
                                                                                     ACTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCA         >  SRR4302547.6059584/1‑94 (MQ=60)
                                                                                      CTTTTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAA        >  SRR4302547.4821943/1‑94 (MQ=60)
                                                                                       TTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAC       <  SRR4302547.3431674/94‑1 (MQ=60)
                                                                                       TTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAC       <  SRR4302547.567729/94‑1 (MQ=60)
                                                                                         TTGCTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGC     <  SRR4302547.5933936/94‑1 (MQ=60)
                                                                                            CTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTA  >  SRR4302547.6606515/1‑94 (MQ=60)
                                                                                            CTGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTA  <  SRR4302547.5010219/94‑1 (MQ=60)
                                                                                             |                                                                                            
GGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTA  >  NC_000913/2235024‑2235209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑