Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,241,691	G→A	intergenic (‑19/‑239)	folE ← / → yeiG	GTP cyclohydrolase I/putative esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,241,691	0	G	A	83.8%	581.4 / 86.5	216	intergenic (‑19/‑239)	folE/yeiG	GTP cyclohydrolase I/putative esterase
Reads supporting (aligned to +/- strand): ref base G (18/17); new base A (89/92); total (107/109)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 229 total aligned reads displayed.

CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT  >  NC_000913/2241601‑2241782

cAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGatg                                                                                           <  2:1716165/93‑1 (MQ=255)

 aGCGTTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAATCAGGGCCGCTTCTTTACTGAGTGATGGTATTTATGTTTTCTCCTGTAGgtgt                                                                                          >  1:6649733/1‑93 (MQ=255)

 aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGatgt                                                                                          >  1:3748153/1‑93 (MQ=255)

 aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGatgt                                                                                          >  1:2682446/1‑93 (MQ=255)

  gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGAtgtg                                                                                         >  2:875174/1‑93 (MQ=255)

  gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGAtgtg                                                                                         >  2:4159310/1‑93 (MQ=255)

   cGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGa                                                                                        <  2:3574203/93‑1 (MQ=255)

        tttCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCt                                                                                   >  1:797667/1‑93 (MQ=255)

        tttCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCt                                                                                   >  1:3281079/1‑93 (MQ=255)

           ccAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCg                                                                                <  1:1869921/93‑1 (MQ=255)

            cAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGc                                                                               >  1:1037659/1‑93 (MQ=255)

            cAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGc                                                                               <  2:3594617/93‑1 (MQ=255)

              gTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGccc                                                                             <  1:2024309/93‑1 (MQ=255)

                 cTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGc                                                                          <  2:2240633/93‑1 (MQ=255)

                 cTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGc                                                                          <  1:536082/93‑1 (MQ=255)

                  tCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCg                                                                         <  2:4058947/93‑1 (MQ=255)

                  tCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCg                                                                         <  1:1557685/93‑1 (MQ=255)

                   cgcgCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGt                                                                        <  2:2930043/93‑1 (MQ=255)

                      gcAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTggg                                                                     >  2:6397314/1‑93 (MQ=255)

                      gcAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTggg                                                                     <  1:2175064/93‑1 (MQ=255)

                       cAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTgggg                                                                    <  2:4186065/93‑1 (MQ=255)

                        aactaactCTTCATCAACCAGCGCCCCTTCTGTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTggggg                                                                   <  2:3305395/93‑1 (MQ=255)

                        aaCTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTggggg                                                                   >  2:2576679/1‑93 (MQ=255)

                        aaCTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTggggg                                                                   >  1:1275479/1‑93 (MQ=255)

                         aCTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGc                                                                  >  2:3991067/1‑93 (MQ=255)

                          cTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCa                                                                 <  1:291083/93‑1 (MQ=255)

                           tAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCaa                                                                <  2:6342073/93‑1 (MQ=255)

                           tAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCaa                                                                <  1:4728318/93‑1 (MQ=255)

                            aaCGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCaaa                                                               >  1:352018/1‑93 (MQ=255)

                            aaCGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCaaa                                                               >  1:4402280/1‑93 (MQ=255)

                             aCGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                              <  2:2853474/93‑1 (MQ=255)

                              cGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGt                                                             <  1:2263613/93‑1 (MQ=255)

                                cTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                           >  2:1416024/1‑93 (MQ=255)

                                    aTGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                       <  2:1160219/93‑1 (MQ=255)

                                    aTGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                       >  1:2557924/1‑93 (MQ=255)

                                    aTGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                       <  1:4013459/93‑1 (MQ=255)

                                      gAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGc                                                     <  1:5526675/93‑1 (MQ=255)

                                         ccAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGa                                                  >  1:1628950/1‑93 (MQ=255)

                                         ccAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGa                                                  <  2:1414653/93‑1 (MQ=255)

                                         ccAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGa                                                  <  1:5736437/93‑1 (MQ=255)

                                         ccAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGa                                                  <  1:1762732/93‑1 (MQ=255)

                                          cAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAt                                                 >  2:4449325/1‑93 (MQ=255)

                                            gggCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAttt                                               >  1:3389404/1‑93 (MQ=255)

                                            gggCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAttt                                               >  2:1391063/1‑93 (MQ=255)

                                            gggCCGCTTCTTTACTGAGAGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGCTATTAAGCTGAttt                                               >  1:3046130/1‑93 (MQ=255)

                                              gCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTaa                                             >  1:5712508/1‑92 (MQ=255)

                                              gCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAc                                             >  2:661465/1‑93 (MQ=255)

                                               ccGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACa                                            <  2:1216787/93‑1 (MQ=255)

                                                cGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAg                                           >  2:5658651/1‑93 (MQ=255)

                                                   ttcttTACTGAGTGTTGGCATTTATGACTTCTCCTGCAGATGTGACGCCTCAGCCCTGCGTGTGAGCGACGTTATTAAGCTGATTTACAGCCt                                        >  1:6174780/1‑93 (MQ=255)

                                                   ttcttTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCt                                        >  2:1889072/1‑93 (MQ=255)

                                                     cttTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGa                                      >  1:4284976/1‑93 (MQ=255)

                                                     cttTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGa                                      >  1:1218001/1‑93 (MQ=255)

                                                      tttACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGat                                     >  2:3828133/1‑93 (MQ=255)

                                                       ttACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatt                                    <  1:1840533/93‑1 (MQ=255)

                                                       ttACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatt                                    <  1:5056844/93‑1 (MQ=255)

                                                         aCTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattat                                  >  2:1651097/1‑93 (MQ=255)

                                                         aCTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattat                                  >  1:1506735/1‑93 (MQ=255)

                                                          cTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattatt                                 <  2:6474303/93‑1 (MQ=255)

                                                           tGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtg                                >  2:4238613/1‑93 (MQ=255)

                                                            gAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtgt                               <  1:3883796/93‑1 (MQ=255)

                                                            gAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtgt                               >  1:1184881/1‑93 (MQ=255)

                                                            gAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtgt                               >  1:2434770/1‑93 (MQ=255)

                                                                gATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGt                           <  1:4559676/93‑1 (MQ=255)

                                                                 aTGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTg                          <  2:1659050/93‑1 (MQ=255)

                                                                  tGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGa                         <  2:3430334/93‑1 (MQ=255)

                                                                   ggCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAg                        >  2:214226/1‑93 (MQ=255)

                                                                    gCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAgg                       >  2:2319673/1‑93 (MQ=255)

                                                                    gCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAgg                       >  2:2968939/1‑93 (MQ=255)

                                                                    gCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAgg                       <  1:5468341/93‑1 (MQ=255)

                                                                     cATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggc                      <  2:2610899/93‑1 (MQ=255)

                                                                     cATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggc                      <  1:5952347/93‑1 (MQ=255)

                                                                       tttATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcgg                    <  1:1383387/93‑1 (MQ=255)

                                                                       tttATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcgg                    >  2:4331010/1‑93 (MQ=255)

                                                                        ttATGATTTCTCCTGCAGGTTTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcggc                   <  2:5553565/93‑1 (MQ=255)

                                                                        ttATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcggc                   >  2:5423952/1‑93 (MQ=255)

                                                                        ttATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcggc                   >  2:4843194/1‑93 (MQ=255)

                                                                         tATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGgcggcg                  >  1:6426657/1‑93 (MQ=255)

                                                                         tATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGgcggcg                  >  1:3782525/1‑93 (MQ=255)

                                                                          aTGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGgcgc                 <  1:5871069/93‑1 (MQ=255)

                                                                           tGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGca                >  2:528806/1‑93 (MQ=255)

                                                                           tGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGca                <  1:5105933/93‑1 (MQ=255)

                                                                           tGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGca                <  1:3868977/93‑1 (MQ=255)

                                                                             aTTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGcaca              <  1:325331/93‑1 (MQ=255)

                                                                             aTTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGcaca              <  2:5070596/93‑1 (MQ=255)

                                                                              tttCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt             >  2:160095/1‑93 (MQ=255)

                                                                               ttCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATa            >  2:4742528/1‑93 (MQ=255)

                                                                                tctcCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATaa           >  2:517907/1‑93 (MQ=255)

                                                                                tctcCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATaa           <  2:5797114/93‑1 (MQ=255)

                                                                                tctcCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATaa           <  1:2654266/93‑1 (MQ=255)

                                                                                 ctcCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAt          <  2:1037659/93‑1 (MQ=255)

                                                                                  tcCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATc         >  2:5205308/1‑93 (MQ=255)

                                                                                    cTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCa       >  1:3141748/1‑93 (MQ=255)

                                                                                     tGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAg      <  2:5950941/93‑1 (MQ=255)

                                                                                     tGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAg      <  2:1103781/93‑1 (MQ=255)

                                                                                     tGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAg      >  2:3777723/1‑93 (MQ=255)

                                                                                      gCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt     >  2:4607854/1‑93 (MQ=255)

                                                                                      gCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt     <  1:412547/93‑1 (MQ=255)

                                                                                        aGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAt   >  1:863733/1‑93 (MQ=255)

                                                                                         ggTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAtt  >  2:793099/1‑93 (MQ=255)

CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT  >  NC_000913/2241601‑2241782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  >  NC_000913/2241598‑2241784

GCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGG                                                                                               <  SRR4302547.1390845/94‑1 (MQ=60)

 CGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGAT                                                                                              <  SRR4302547.4251688/94‑1 (MQ=60)

  GCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTG                                                                                             >  SRR4302547.6069577/1‑94 (MQ=60)

   TAGCGTTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAATCAGGGCCGCTTCTTTACTGAGTGATGGTATTTATGTTTTCTCCTGTAGGTGT                                                                                            >  SRR4302547.6788296/1‑94 (MQ=60)

   CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                            <  SRR4302547.517654/94‑1 (MQ=60)

   CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGT                                                                                            >  SRR4302547.2736307/1‑94 (MQ=60)

   CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGT                                                                                            >  SRR4302547.3824666/1‑94 (MQ=60)

    AGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTG                                                                                           >  SRR4302547.2224275/1‑94 (MQ=60)

       GGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACG                                                                                        <  SRR4302547.921736/94‑1 (MQ=60)

         TGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCC                                                                                      >  SRR4302547.3837782/1‑94 (MQ=60)

          GTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCT                                                                                     >  SRR4302547.4414545/1‑94 (MQ=60)

          GTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCT                                                                                     >  SRR4302547.812765/1‑94 (MQ=60)

          GTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCT                                                                                     >  SRR4302547.4188755/1‑94 (MQ=60)

          GTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCT                                                                                     >  SRR4302547.3346860/1‑94 (MQ=60)

          GTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCT                                                                                     >  SRR4302547.3299739/1‑94 (MQ=60)

            TTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCC                                                                                   <  SRR4302547.1592817/94‑1 (MQ=60)

              CCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGC                                                                                 >  SRR4302547.1058101/1‑94 (MQ=60)

              CCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGC                                                                                 <  SRR4302547.1906850/94‑1 (MQ=60)

               CAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCC                                                                                >  SRR4302547.2085992/1‑94 (MQ=60)

                 GTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCT                                                                              <  SRR4302547.2064622/94‑1 (MQ=60)

                  TCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTG                                                                             <  SRR4302547.3083381/94‑1 (MQ=60)

                  TCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTG                                                                             <  SRR4302547.5639611/94‑1 (MQ=60)

                  TCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTG                                                                             >  SRR4302547.2547892/1‑94 (MQ=60)

                  TCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTG                                                                             >  SRR4302547.3517171/1‑94 (MQ=60)

                    CTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                           <  SRR4302547.4804767/94‑1 (MQ=60)

                    CTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCG                                                                           <  SRR4302547.545766/94‑1 (MQ=60)

                    CTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCG                                                                           <  SRR4302547.5023749/94‑1 (MQ=60)

                    CTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCG                                                                           >  SRR4302547.6431713/1‑94 (MQ=60)

                    CTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGCGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCG                                                                           >  SRR4302547.3960539/1‑94 (MQ=60)

                     TCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGT                                                                          <  SRR4302547.1588712/94‑1 (MQ=60)

                      CGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTG                                                                         >  SRR4302547.5518929/1‑94 (MQ=60)

                      CGCGCAACTAACGCCTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTG                                                                         <  SRR4302547.1208847/94‑1 (MQ=60)

                        CGCATCTAGCTCTTCATGAGCCAGGGCCGCTTCTTTACTGAGTGATGTCTTTTATGATTTCTCCTGCTGGTGTGACGCCTCCGCCCTGCGTGGG                                                                       <  SRR4302547.2455235/94‑1 (MQ=60)

                        CGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGG                                                                       >  SRR4302547.4450352/1‑94 (MQ=60)

                         GCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGG                                                                      <  SRR4302547.1363997/94‑1 (MQ=60)

                         GCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGG                                                                      <  SRR4302547.2218612/94‑1 (MQ=60)

                         GAAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACACCTCCGCCCCGCGTGGGA                                                                      <  SRR4302547.2472976/94‑1 (MQ=60)

                          CAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGG                                                                     <  SRR4302547.5975478/94‑1 (MQ=60)

                          CAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGG                                                                     >  SRR4302547.1301167/1‑94 (MQ=60)

                           AACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGC                                                                    <  SRR4302547.1809228/94‑1 (MQ=60)

                            ACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCA                                                                   >  SRR4302547.1946178/1‑94 (MQ=60)

                             CTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAA                                                                  <  SRR4302547.296489/94‑1 (MQ=60)

                              TAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAA                                                                 >  SRR4302547.4492938/1‑94 (MQ=60)

                              TAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAA                                                                 <  SRR4302547.4826298/94‑1 (MQ=60)

                              TAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAA                                                                 >  SRR4302547.358561/1‑94 (MQ=60)

                               AACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAG                                                                >  SRR4302547.5460800/1‑94 (MQ=60)

                                ACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGT                                                               <  SRR4302547.705669/94‑1 (MQ=60)

                                 CGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTT                                                              <  SRR4302547.2309362/94‑1 (MQ=60)

                                   CTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTAT                                                            <  SRR4302547.3955728/94‑1 (MQ=60)

                                      CATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAA                                                         >  SRR4302547.3963772/1‑94 (MQ=60)

                                      CATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAA                                                         >  SRR4302547.2609584/1‑94 (MQ=60)

                                       ATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                        <  SRR4302547.6354661/94‑1 (MQ=60)

                                       ATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                        <  SRR4302547.3264369/94‑1 (MQ=60)

                                       ATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                        <  SRR4302547.4095035/94‑1 (MQ=60)

                                         GAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCT                                                      <  SRR4302547.5640735/94‑1 (MQ=60)

                                          AACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTG                                                     <  SRR4302547.3324008/94‑1 (MQ=60)

                                          AACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTG                                                     >  SRR4302547.5780529/1‑94 (MQ=60)

                                          AACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTG                                                     >  SRR4302547.1954533/1‑94 (MQ=60)

                                           ACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGA                                                    <  SRR4302547.1407001/94‑1 (MQ=60)

                                           ACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGA                                                    >  SRR4302547.1661167/1‑94 (MQ=60)

                                            CCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                                   <  SRR4302547.5854955/94‑1 (MQ=60)

                                            CCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                                   <  SRR4302547.1797695/94‑1 (MQ=60)

                                              AGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTT                                                 >  SRR4302547.3458357/1‑94 (MQ=60)

                                              AGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTT                                                 >  SRR4302547.1195072/1‑94 (MQ=60)

                                              AGGGCCGCTTCTTTACTGAGAGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGCTATTAAGCTGATTT                                                 >  SRR4302547.3106746/1‑94 (MQ=60)

                                               GGGCCGCTTCTTTACTGAGGGATGGCATTTATGCATTCTCCTGCAGATGTGACGAATACGCCCTGCGTGTGTGCAGAGTTATTAAGCTGATTTA                                                >  SRR4302547.4541927/1‑94 (MQ=42)

                                                GGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAA                                               >  SRR4302547.5830494/1‑94 (MQ=60)

                                                 GCTGCTTCTTTACTGAGTCAGTGCATTAAAGATTTCTCATGCAGATGTGACGCCTCCGCCCTGCGTGAGGGCAAAGTTATTAAGCTGATTTACA                                              <  SRR4302547.950061/94‑1 (MQ=57)

                                                 GCCGCTTCTGTACTGAGTGACGGCATTTATGATTTCTCCTGCGGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACA                                              >  SRR4302547.6582836/1‑94 (MQ=60)

                                                   CGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGC                                            <  SRR4302547.1647388/94‑1 (MQ=60)

                                                    GCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCC                                           >  SRR4302547.4864669/1‑94 (MQ=60)

                                                    GCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCC                                           >  SRR4302547.5947877/1‑94 (MQ=60)

                                                     CTTCTTTACTGAGTGTTGGCATTTATGACTTCTCCTGCAGATGTGACGCCTCAGCCCTGCGTGTGAGCGACGTTATTAAGCTGATTTACAGCCT                                          >  SRR4302547.6302948/1‑94 (MQ=60)

                                                       TCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGA                                        >  SRR4302547.4373028/1‑94 (MQ=60)

                                                       TCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGA                                        >  SRR4302547.1242586/1‑94 (MQ=60)

                                                         TTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATT                                      <  SRR4302547.5843781/94‑1 (MQ=60)

                                                          TTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTA                                     <  SRR4302547.5161213/94‑1 (MQ=60)

                                                          TTACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTA                                     <  SRR4302547.1876906/94‑1 (MQ=60)

                                                           TACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTAT                                    >  SRR4302547.1536769/1‑94 (MQ=60)

                                                            ACTGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATT                                   <  SRR4302547.1552793/94‑1 (MQ=60)

                                                              TGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGT                                 >  SRR4302547.2484022/1‑94 (MQ=60)

                                                              TGAGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGT                                 >  SRR4302547.1208816/1‑94 (MQ=60)

                                                               GAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTG                                <  SRR4302547.3962798/94‑1 (MQ=60)

                                                                AGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGC                               <  SRR4302547.611795/94‑1 (MQ=60)

                                                                AGTGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGC                               >  SRR4302547.2832807/1‑94 (MQ=60)

                                                                  TGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGT                             <  SRR4302547.4520875/94‑1 (MQ=60)

                                                                  TGATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGT                             <  SRR4302547.4299699/94‑1 (MQ=60)

                                                                   GATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTG                            <  SRR4302547.1877991/94‑1 (MQ=60)

                                                                   GATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTG                            <  SRR4302547.4654009/94‑1 (MQ=60)

                                                                   GATGGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTG                            <  SRR4302547.1739585/94‑1 (MQ=60)

                                                                    ATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGA                           <  SRR4302547.1252988/94‑1 (MQ=60)

                                                                     TGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAG                          <  SRR4302547.3676325/94‑1 (MQ=60)

                                                                      GGCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTCCGTGAGG                         >  SRR4302547.845828/1‑94 (MQ=60)

                                                                       GCATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGC                        <  SRR4302547.5581306/94‑1 (MQ=60)

                                                                        CATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCG                       >  SRR4302547.6726420/1‑94 (MQ=60)

                                                                        CATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCG                       <  SRR4302547.6075783/94‑1 (MQ=60)

                                                                        CATTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCG                       <  SRR4302547.2669251/94‑1 (MQ=60)

                                                                          TTTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGC                     <  SRR4302547.1411094/94‑1 (MQ=60)

                                                                           TTATGATTTCTCCTGCAGATGTTACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAGGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCG                    <  SRR4302547.2606803/94‑1 (MQ=60)

                                                                           TTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCG                    >  SRR4302547.6560642/1‑94 (MQ=60)

                                                                           TTATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCG                    >  SRR4302547.3859655/1‑94 (MQ=60)

                                                                             ATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCA                  >  SRR4302547.3741431/1‑94 (MQ=60)

                                                                             ATGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCA                  <  SRR4302547.5992595/94‑1 (MQ=60)

                                                                              TGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCAC                 <  SRR4302547.3947741/94‑1 (MQ=60)

                                                                              TGATTTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCAC                 <  SRR4302547.5211161/94‑1 (MQ=60)

                                                                                ATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAT               <  SRR4302547.331389/94‑1 (MQ=60)

                                                                                  TTCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCAAATAA             >  SRR4302547.2328927/1‑94 (MQ=60)

                                                                                   TCTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAT            <  SRR4302547.2707617/94‑1 (MQ=60)

                                                                                    CTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATC           >  SRR4302547.5523552/1‑94 (MQ=60)

                                                                                    CTCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATC           >  SRR4302547.3945197/1‑94 (MQ=60)

                                                                                     TCCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCC          <  SRR4302547.2714624/94‑1 (MQ=60)

                                                                                      CCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCA         >  SRR4302547.3204161/1‑94 (MQ=60)

                                                                                      CCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCA         >  SRR4302547.3347027/1‑94 (MQ=60)

                                                                                      CCTGCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCA         <  SRR4302547.1420621/94‑1 (MQ=60)

                                                                                         GCAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTA      <  SRR4302547.420202/94‑1 (MQ=60)

                                                                                          CAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT     >  SRR4302547.880254/1‑94 (MQ=60)

                                                                                          CAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT     <  SRR4302547.3139409/94‑1 (MQ=60)

                                                                                          CAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT     >  SRR4302547.661836/1‑94 (MQ=60)

                                                                                          CAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT     >  SRR4302547.6527408/1‑94 (MQ=60)

                                                                                          CAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT     >  SRR4302547.1218311/1‑94 (MQ=60)

                                                                                          CAGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT     <  SRR4302547.2743901/94‑1 (MQ=60)

                                                                                           AGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT    >  SRR4302547.6441081/1‑94 (MQ=60)

                                                                                           AGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT    <  SRR4302547.5155712/94‑1 (MQ=60)

                                                                                           AGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT    <  SRR4302547.3347173/94‑1 (MQ=60)

                                                                                           AGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT    >  SRR4302547.5304005/1‑94 (MQ=60)

                                                                                           AGATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT    >  SRR4302547.1022043/1‑94 (MQ=60)

                                                                                             ATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  >  SRR4302547.4565028/1‑94 (MQ=60)

                                                                                             ATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  <  SRR4302547.4148549/94‑1 (MQ=60)

                                                                                             ATGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  >  SRR4302547.5964971/1‑94 (MQ=60)

GCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  >  NC_000913/2241598‑2241784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`