Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,433	T→C	R195R (CGT→CGC)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,433	0	T	C	81.8%	453.4 / 99.0	203	R195R (CGT→CGC)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/19); new base C (80/86); total (98/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 217 total aligned reads displayed.

CTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCA  >  NC_000913/2266341‑2266525

cTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGt                                                                                              <  2:3510898/93‑1 (MQ=255)

  aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCg                                                                                            >  2:3068445/1‑93 (MQ=255)

  aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCg                                                                                            <  2:5362687/93‑1 (MQ=255)

      ggTTGGTGGCAGCAAAATGATGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAAt                                                                                        <  1:1371725/92‑1 (MQ=255)

       gTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAAtt                                                                                       >  2:2189331/1‑93 (MQ=255)

        ttGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAAttt                                                                                      >  1:5125847/1‑93 (MQ=255)

          ggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAg                                                                                    >  1:3233388/1‑93 (MQ=255)

           gtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGc                                                                                   <  2:4433928/93‑1 (MQ=255)

            tggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGcc                                                                                  <  1:4465895/93‑1 (MQ=255)

                agcaAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGt                                                                              <  1:2129693/93‑1 (MQ=255)

                   aaaaTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGc                                                                           >  2:4919813/1‑93 (MQ=255)

                   aaaaTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGc                                                                           >  2:1369234/1‑93 (MQ=255)

                    aaaTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGcc                                                                          <  2:5662652/93‑1 (MQ=255)

                    aaaTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGcc                                                                          >  1:2976737/1‑93 (MQ=255)

                     aaTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGccc                                                                         >  2:5725298/1‑93 (MQ=255)

                     aaTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGccc                                                                         <  2:3049311/93‑1 (MQ=255)

                      aTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCg                                                                        >  1:176123/1‑93 (MQ=255)

                      aTGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCg                                                                        >  1:1071071/1‑93 (MQ=255)

                       tggtggCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGc                                                                       <  2:2418870/93‑1 (MQ=255)

                       tggtggCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGCTGCCCGc                                                                       <  1:5331834/93‑1 (MQ=255)

                         gtggCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCa                                                                     <  1:401713/93‑1 (MQ=255)

                         gtggCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCa                                                                     >  1:4641142/1‑93 (MQ=255)

                             cGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAgcgc                                                                 <  1:1174134/93‑1 (MQ=255)

                             cGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAgcgc                                                                 >  1:3457421/1‑93 (MQ=255)

                              gATCGACAATGAATTCACAGTGAGCATGGGAGAGTTGACGGTCATCTTCTGGATTGGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGcgcc                                                                <  2:1619894/93‑1 (MQ=255)

                               aTCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGcgccg                                                               >  1:2433799/1‑93 (MQ=255)

                               aTCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGcgccg                                                               >  1:3366158/1‑93 (MQ=255)

                               aTCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGcgccg                                                               >  1:5906389/1‑93 (MQ=255)

                                tCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc                                                              <  1:3555409/93‑1 (MQ=255)

                                tCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc                                                              >  2:2918477/1‑93 (MQ=255)

                                tCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc                                                              >  2:4969502/1‑93 (MQ=255)

                                 cGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCcgcg                                                             <  2:4113520/93‑1 (MQ=255)

                                   aCAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                                                           <  2:6233714/93‑1 (MQ=255)

                                   aCAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                                                           >  1:5977251/1‑93 (MQ=255)

                                   aCAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                                                           >  2:6433626/1‑93 (MQ=255)

                                    cAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt                                                          <  2:4532401/93‑1 (MQ=255)

                                    cAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt                                                          <  1:6285639/93‑1 (MQ=255)

                                     aattaattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAtt                                                         >  1:6364393/1‑93 (MQ=255)

                                      attaattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtc                                                        <  2:2576584/93‑1 (MQ=255)

                                      attaattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtc                                                        <  2:4077698/93‑1 (MQ=255)

                                      attaattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtc                                                        <  1:5801895/93‑1 (MQ=255)

                                        taattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtctc                                                      >  2:845548/1‑93 (MQ=255)

                                        taattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtctc                                                      >  2:973520/1‑93 (MQ=255)

                                           ttCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCtcatc                                                   >  1:4728496/1‑93 (MQ=255)

                                           ttCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCtcatc                                                   <  2:6075658/93‑1 (MQ=255)

                                           ttCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCtcatc                                                   >  2:4330759/1‑93 (MQ=255)

                                           ttCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCtcatc                                                   >  1:2505224/1‑93 (MQ=255)

                                              acaGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGc                                                >  1:5037077/1‑93 (MQ=255)

                                                aGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCAt                                              <  1:625515/93‑1 (MQ=255)

                                                 gTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATg                                             >  2:2174005/1‑93 (MQ=255)

                                                 gTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATg                                             >  1:3773522/1‑93 (MQ=255)

                                                   gAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTc                                           <  1:5055797/93‑1 (MQ=255)

                                                   gAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTc                                           <  1:1860568/93‑1 (MQ=255)

                                                   gAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTc                                           <  1:2320981/93‑1 (MQ=255)

                                                    aGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCg                                          >  2:5152633/1‑93 (MQ=255)

                                                    aGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCg                                          >  1:2156640/1‑93 (MQ=255)

                                                     gCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTATCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGt                                         >  2:6642049/1‑93 (MQ=255)

                                                     gCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGt                                         <  2:753627/93‑1 (MQ=255)

                                                      cATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTg                                        <  2:2046327/93‑1 (MQ=255)

                                                        tGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaa                                      >  1:1226864/1‑93 (MQ=255)

                                                        tGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaa                                      >  2:5549033/1‑93 (MQ=255)

                                                           gAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaa                                   >  2:4019476/1‑93 (MQ=255)

                                                            aaaGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaaa                                  <  1:6499801/93‑1 (MQ=255)

                                                             aaGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAg                                 <  1:4520103/93‑1 (MQ=255)

                                                              aGTTGACGGTCATCTTTTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAgg                                <  2:578847/93‑1 (MQ=255)

                                                              aGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAgg                                <  1:848071/93‑1 (MQ=255)

                                                              aGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAgg                                <  1:307875/93‑1 (MQ=255)

                                                              aGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTAATCAGCATGTCGTGAAAAAAgg                                <  1:968953/93‑1 (MQ=255)

                                                               gTTGACGGTCATGTTCTGTATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAggg                               <  2:6584044/93‑1 (MQ=255)

                                                                ttGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGt                              <  2:2866273/93‑1 (MQ=255)

                                                                ttGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGt                              <  1:1981808/93‑1 (MQ=255)

                                                                 tGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGtt                             <  2:6302409/93‑1 (MQ=255)

                                                                 tGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGtt                             <  2:6374687/93‑1 (MQ=255)

                                                                  gACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTa                            <  1:1629017/93‑1 (MQ=255)

                                                                  gACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTa                            >  1:1685737/1‑93 (MQ=255)

                                                                  aaCGGTCATCTTCTGGATTTGCCGCGCCGGAATGTAGCAGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAACGGTTa                            <  1:1933544/92‑1 (MQ=255)

                                                                     ggTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTagca                         >  1:4981939/1‑93 (MQ=255)

                                                                     ggTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTagca                         <  1:487055/93‑1 (MQ=255)

                                                                      gTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTagcag                        <  1:6576837/93‑1 (MQ=255)

                                                                      gTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTagcag                        <  1:4808707/93‑1 (MQ=255)

                                                                      gTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTagcag                        >  1:4254826/1‑93 (MQ=255)

                                                                        cATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCg                      >  1:1442298/1‑93 (MQ=255)

                                                                        cATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCg                      <  1:2765051/93‑1 (MQ=255)

                                                                        cATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCg                      >  2:6049425/1‑93 (MQ=255)

                                                                         aTCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGt                     >  2:3820526/1‑93 (MQ=255)

                                                                          tcttctGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGtt                    >  2:1937999/1‑93 (MQ=255)

                                                                           cttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTa                   <  1:5471726/93‑1 (MQ=255)

                                                                           cttctGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTa                   <  2:1415895/93‑1 (MQ=255)

                                                                            ttctGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTaa                  <  2:498467/93‑1 (MQ=255)

                                                                            ttctGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTaa                  <  1:863053/93‑1 (MQ=255)

                                                                                ggATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCt              <  1:735588/93‑1 (MQ=255)

                                                                                ggATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCt              <  1:786025/93‑1 (MQ=255)

                                                                                ggATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCt              >  2:4165090/1‑93 (MQ=255)

                                                                                ggATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCt              <  1:3923944/93‑1 (MQ=255)

                                                                                    ttGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCa          <  1:1550692/93‑1 (MQ=255)

                                                                                     tGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCag         <  1:973520/93‑1 (MQ=255)

                                                                                      gccgcgccgcAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCaga        <  2:4847668/93‑1 (MQ=255)

                                                                                      gccgcgccgcAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCaga        >  2:3437679/1‑93 (MQ=255)

                                                                                         gcgTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCa     >  1:288379/1‑93 (MQ=255)

                                                                                            tCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCa  <  1:3682261/93‑1 (MQ=255)

CTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCA  >  NC_000913/2266341‑2266525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAA  >  NC_000913/2266340‑2266526

GCTACAGCGTTGGTGGGAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGT                                                                                               <  SRR4302547.3210716/94‑1 (MQ=60)

 CTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCC                                                                                              >  SRR4302547.5936565/1‑94 (MQ=60)

       GGTTGGTGGCAGCAAAATGATGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATT                                                                                        <  SRR4302547.1399244/94‑1 (MQ=60)

       CGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATT                                                                                        <  SRR4302547.3744865/94‑1 (MQ=60)

        GTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTT                                                                                       <  SRR4302547.4558957/94‑1 (MQ=60)

        GTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTT                                                                                       >  SRR4302547.5231469/1‑94 (MQ=60)

          TGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAG                                                                                     >  SRR4302547.3297923/1‑94 (MQ=60)

            GTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCC                                                                                   <  SRR4302547.5276162/94‑1 (MQ=60)

             TGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCG                                                                                  <  SRR4302547.4558169/94‑1 (MQ=60)

             TGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCG                                                                                  >  SRR4302547.5481980/1‑94 (MQ=60)

              GGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGA                                                                                 <  SRR4302547.3882255/94‑1 (MQ=60)

                 AGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTT                                                                              <  SRR4302547.2172127/94‑1 (MQ=60)

                    AAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCC                                                                           >  SRR4302547.3035962/1‑94 (MQ=60)

                     AAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCC                                                                          <  SRR4302547.823075/94‑1 (MQ=60)

                     AAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCC                                                                          >  SRR4302547.5222011/1‑94 (MQ=60)

                     AAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCC                                                                          <  SRR4302547.1128881/94‑1 (MQ=60)

                      AATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCG                                                                         >  SRR4302547.4918462/1‑94 (MQ=60)

                      AATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCG                                                                         >  SRR4302547.179372/1‑94 (MQ=60)

                      AATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCG                                                                         >  SRR4302547.1092410/1‑94 (MQ=60)

                        TGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCC                                                                       >  SRR4302547.991225/1‑94 (MQ=60)

                        TGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGCTGCCCGCC                                                                       <  SRR4302547.5441726/94‑1 (MQ=60)

                         GGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCA                                                                      >  SRR4302547.4737263/1‑94 (MQ=60)

                         GGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCA                                                                      <  SRR4302547.668804/94‑1 (MQ=60)

                          GTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAG                                                                     <  SRR4302547.409176/94‑1 (MQ=60)

                          GTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAG                                                                     >  SRR4302547.5141945/1‑94 (MQ=60)

                           TGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGC                                                                    <  SRR4302547.1885998/94‑1 (MQ=60)

                             GCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGC                                                                  >  SRR4302547.3527911/1‑94 (MQ=60)

                              CGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCC                                                                 <  SRR4302547.1197864/94‑1 (MQ=60)

                               GATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCG                                                                >  SRR4302547.2483039/1‑94 (MQ=60)

                               GATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCG                                                                >  SRR4302547.3434524/1‑94 (MQ=60)

                               GATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCG                                                                >  SRR4302547.6028711/1‑94 (MQ=60)

                               GATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCG                                                                >  SRR4302547.2186122/1‑94 (MQ=60)

                                 TCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCG                                                              <  SRR4302547.3627965/94‑1 (MQ=60)

                                  CGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGC                                                             <  SRR4302547.3794418/94‑1 (MQ=60)

                                  CGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGC                                                             >  SRR4302547.871020/1‑94 (MQ=60)

                                   GACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCA                                                            >  SRR4302547.6101238/1‑94 (MQ=60)

                                    ACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAT                                                           >  SRR4302547.2121341/1‑94 (MQ=60)

                                     CAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATT                                                          >  SRR4302547.4892109/1‑94 (MQ=60)

                                     CAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATT                                                          >  SRR4302547.6497161/1‑94 (MQ=60)

                                     CAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATT                                                          <  SRR4302547.6416232/94‑1 (MQ=60)

                                     CAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCATCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATT                                                          >  SRR4302547.4695972/1‑94 (MQ=60)

                                      AATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTC                                                         >  SRR4302547.299964/1‑94 (MQ=60)

                                       ATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCT                                                        <  SRR4302547.5921929/94‑1 (MQ=60)

                                        TTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTC                                                       >  SRR4302547.2535162/1‑94 (MQ=60)

                                         TAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCA                                                      <  SRR4302547.5115321/94‑1 (MQ=60)

                                          AATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCAT                                                     >  SRR4302547.6162499/1‑94 (MQ=60)

                                          AATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCAT                                                     <  SRR4302547.3080577/94‑1 (MQ=60)

                                           ATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATC                                                    >  SRR4302547.4826485/1‑94 (MQ=60)

                                           ATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATC                                                    >  SRR4302547.2555859/1‑94 (MQ=60)

                                            TTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCA                                                   <  SRR4302547.3458142/94‑1 (MQ=60)

                                            TTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCTTTCTCATCA                                                   <  SRR4302547.6624661/94‑1 (MQ=60)

                                            TTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCA                                                   >  SRR4302547.6492142/1‑94 (MQ=60)

                                              CACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGC                                                 >  SRR4302547.5141115/1‑94 (MQ=60)

                                               ACAGTGAGCATGTGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCA                                                <  SRR4302547.2701938/94‑1 (MQ=60)

                                                 AGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATG                                              <  SRR4302547.2394376/94‑1 (MQ=60)

                                                 AGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATG                                              >  SRR4302547.5786577/1‑94 (MQ=60)

                                                 AGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATG                                              >  SRR4302547.3850484/1‑94 (MQ=60)

                                                 AGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATG                                              <  SRR4302547.637067/94‑1 (MQ=60)

                                                 AGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATG                                              >  SRR4302547.915265/1‑94 (MQ=60)

                                                   TGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTC                                            <  SRR4302547.86676/94‑1 (MQ=60)

                                                    GAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCG                                           <  SRR4302547.1233678/94‑1 (MQ=60)

                                                    GAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCG                                           <  SRR4302547.5160152/94‑1 (MQ=60)

                                                    GAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCG                                           <  SRR4302547.1897301/94‑1 (MQ=60)

                                                    GAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCG                                           <  SRR4302547.2368021/94‑1 (MQ=60)

                                                    GAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCG                                           >  SRR4302547.2199731/1‑94 (MQ=60)

                                                     AGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGT                                          >  SRR4302547.2355670/1‑94 (MQ=60)

                                                       CATGGGAAAGTTGAGGGTCATCTTCTGGGTTTGCGGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCACGTCGTGA                                        >  SRR4302547.1569454/1‑94 (MQ=60)

                                                       CATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGA                                        >  SRR4302547.59354/1‑94 (MQ=60)

                                                       CATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGA                                        >  SRR4302547.1708499/1‑94 (MQ=60)

                                                       CATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGA                                        >  SRR4302547.2002581/1‑94 (MQ=60)

                                                        ATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAA                                       >  SRR4302547.1251619/1‑94 (MQ=60)

                                                        ATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAA                                       <  SRR4302547.358970/94‑1 (MQ=60)

                                                         TGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAA                                      <  SRR4302547.794747/94‑1 (MQ=60)

                                                             AAAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAG                                  <  SRR4302547.6635085/94‑1 (MQ=60)

                                                              AAGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGG                                 <  SRR4302547.4613490/94‑1 (MQ=60)

                                                               AGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGG                                <  SRR4302547.313608/94‑1 (MQ=60)

                                                               AGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGG                                >  SRR4302547.3410988/1‑94 (MQ=60)

                                                               AGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGG                                <  SRR4302547.864268/94‑1 (MQ=60)

                                                               AGTTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTAATCAGCATGTCGTGAAAAAAGGG                                <  SRR4302547.987761/94‑1 (MQ=60)

                                                                GTGGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGT                               <  SRR4302547.5618397/94‑1 (MQ=60)

                                                                 TTGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTT                              <  SRR4302547.2021160/94‑1 (MQ=60)

                                                                  TGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTA                             >  SRR4302547.1718909/1‑94 (MQ=60)

                                                                  TGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTA                             <  SRR4302547.6089768/94‑1 (MQ=60)

                                                                  TGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTA                             <  SRR4302547.1425786/94‑1 (MQ=60)

                                                                  TGACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGAGCCGCGCATTCTCATCAGCATGTGGTGAAAAAAGGGTTA                             >  SRR4302547.4374711/1‑94 (MQ=60)

                                                                   GACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAG                            <  SRR4302547.2911452/94‑1 (MQ=60)

                                                                   GACGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAG                            <  SRR4302547.1661236/94‑1 (MQ=60)

                                                                   AACGGTCATCTTCTGGATTTGCCGCGCCGGAATGTAGCAGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAACGGTTAG                            <  SRR4302547.1971885/94‑1 (MQ=60)

                                                                     CGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTCGCA                          >  SRR4302547.1015266/1‑94 (MQ=60)

                                                                     CGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCA                          >  SRR4302547.5084973/1‑94 (MQ=60)

                                                                     CGGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCA                          <  SRR4302547.1306422/94‑1 (MQ=60)

                                                                      GGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAG                         >  SRR4302547.4341884/1‑94 (MQ=60)

                                                                      GGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAG                         <  SRR4302547.495971/94‑1 (MQ=60)

                                                                      GGTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAG                         <  SRR4302547.354311/94‑1 (MQ=60)

                                                                       GTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGC                        <  SRR4302547.6713763/94‑1 (MQ=60)

                                                                       GTCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGC                        <  SRR4302547.4908378/94‑1 (MQ=60)

                                                                        TCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCG                       >  SRR4302547.1471136/1‑94 (MQ=60)

                                                                        TCATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCG                       <  SRR4302547.292682/94‑1 (MQ=60)

                                                                         CATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGT                      >  SRR4302547.3255871/1‑94 (MQ=60)

                                                                         CATCTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGT                      <  SRR4302547.2820458/94‑1 (MQ=60)

                                                                            CTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAA                   <  SRR4302547.5584759/94‑1 (MQ=60)

                                                                            CTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAA                   >  SRR4302547.5002602/1‑94 (MQ=60)

                                                                            CTTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAA                   >  SRR4302547.1520593/1‑94 (MQ=60)

                                                                             TTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAG                  >  SRR4302547.1611539/1‑94 (MQ=60)

                                                                             TTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAG                  <  SRR4302547.879553/94‑1 (MQ=60)

                                                                             TTCTGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAG                  <  SRR4302547.5031285/94‑1 (MQ=60)

                                                                                TGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCT               <  SRR4302547.6411100/94‑1 (MQ=60)

                                                                                TGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCT               >  SRR4302547.6554830/1‑94 (MQ=60)

                                                                                TGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCT               <  SRR4302547.4105493/94‑1 (MQ=60)

                                                                                TGGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCT               <  SRR4302547.1777112/94‑1 (MQ=60)

                                                                                 GGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTG              <  SRR4302547.800901/94‑1 (MQ=60)

                                                                                 GGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTG              <  SRR4302547.4003736/94‑1 (MQ=60)

                                                                                 GGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTG              <  SRR4302547.749341/94‑1 (MQ=60)

                                                                                 GGATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTG              <  SRR4302547.453921/94‑1 (MQ=60)

                                                                                   ATTTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCC            >  SRR4302547.711384/1‑94 (MQ=60)

                                                                                     TTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG          >  SRR4302547.1933952/1‑94 (MQ=60)

                                                                                     TTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG          <  SRR4302547.1589963/94‑1 (MQ=60)

                                                                                     TTGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG          <  SRR4302547.1581565/94‑1 (MQ=60)

                                                                                      TGCCGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGA         <  SRR4302547.992447/94‑1 (MQ=60)

                                                                                         CGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCA      >  SRR4302547.293733/1‑94 (MQ=60)

                                                                                         CGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCA      <  SRR4302547.4749867/94‑1 (MQ=60)

                                                                                         CGCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCA      <  SRR4302547.4031662/94‑1 (MQ=60)

                                                                                          GCGCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCAT     <  SRR4302547.2137250/94‑1 (MQ=60)

                                                                                            GCCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCA   <  SRR4302547.2004049/94‑1 (MQ=60)

                                                                                             TCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAA  <  SRR4302547.3757431/94‑1 (MQ=60)

GCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAA  >  NC_000913/2266340‑2266526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`