Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,267,467 T→C G198G (GGT→GGC)  b2174 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,267,4670TC87.3% 598.9 / 69.9 221G198G (GGT→GGC) b2174hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/18);  new base C (92/101);  total (102/119)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 228 total aligned reads displayed.

GTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTAC  >  NC_000913/2267375‑2267555
                                                                                            |                                                                                        
gTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGt                                                                                          <  1:6447529/93‑1 (MQ=255)
    cAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGa                                                                                      <  2:945757/93‑1 (MQ=255)
     aGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGAt                                                                                     <  1:4517633/93‑1 (MQ=255)
     aGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGAt                                                                                     >  1:471325/1‑93 (MQ=255)
     aGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGAt                                                                                     <  1:2597362/93‑1 (MQ=255)
       gCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGa                                                                                   >  2:2674456/1‑93 (MQ=255)
        ccTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGAc                                                                                  <  1:2080144/93‑1 (MQ=255)
        ccTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGAc                                                                                  >  1:5926993/1‑93 (MQ=255)
         cTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGAcc                                                                                 <  1:1753343/93‑1 (MQ=255)
          ttATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCg                                                                                <  1:5216271/93‑1 (MQ=255)
          ttATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCg                                                                                >  2:3973609/1‑93 (MQ=255)
           tATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGt                                                                               <  2:2244904/93‑1 (MQ=255)
            aTCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGTCATCATTGTCGGTTCGATGACCGTg                                                                              <  2:6170672/93‑1 (MQ=255)
              cGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGat                                                                            <  1:4894969/93‑1 (MQ=255)
               gCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGata                                                                           >  2:1497046/1‑93 (MQ=255)
                cccTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGatat                                                                          >  2:5426519/1‑93 (MQ=255)
                cccTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGatat                                                                          >  1:3398068/1‑93 (MQ=255)
                 ccTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATAtt                                                                         >  2:1422354/1‑93 (MQ=255)
                   ttattaTTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGa                                                                       <  1:5321914/93‑1 (MQ=255)
                    tattatTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGAt                                                                      >  1:393269/1‑93 (MQ=255)
                      ttattTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCg                                                                    <  2:3299564/93‑1 (MQ=255)
                      ttattTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCg                                                                    <  2:1171682/93‑1 (MQ=255)
                      ttattTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCg                                                                    <  1:2471183/93‑1 (MQ=255)
                          ttttGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGttt                                                                >  1:5206952/1‑93 (MQ=255)
                           tttGTGGTTTTTGCTTTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCTTTGTCGGCTCGATGACCGTGATATTGATCGGtttc                                                               <  2:6270011/93‑2 (MQ=255)
                            ttGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGc                                                              <  1:6109698/93‑1 (MQ=255)
                            ttGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGc                                                              >  2:2384632/1‑93 (MQ=255)
                              gtgGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGccc                                                            <  1:700858/93‑1 (MQ=255)
                              gtgGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGccc                                                            <  1:749396/93‑1 (MQ=255)
                              gtgGTTTTTGCATTTCCCAGAGTAATGATTAGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGGTATTGATCGGTTTTccc                                                            >  1:1362031/1‑93 (MQ=255)
                                 gTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTgg                                                         >  1:1031784/1‑93 (MQ=255)
                                 gTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTgg                                                         >  2:124312/1‑93 (MQ=255)
                                  tttttGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTggg                                                        >  2:3842338/1‑93 (MQ=255)
                                  tttttGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTggg                                                        >  1:2921879/1‑93 (MQ=255)
                                   ttttGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGgt                                                       <  1:3615602/93‑1 (MQ=255)
                                     ttGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGgtgt                                                     >  1:4639981/1‑93 (MQ=255)
                                        gaTTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGc                                                  <  1:2185388/92‑1 (MQ=255)
                                        cATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGc                                                  <  1:6302816/93‑1 (MQ=255)
                                         aTTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCt                                                 <  1:2757978/93‑1 (MQ=255)
                                          tttCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTg                                                >  1:2964025/1‑93 (MQ=255)
                                          tttCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTg                                                <  2:4244593/93‑1 (MQ=255)
                                          tttCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTg                                                >  1:6549510/1‑93 (MQ=255)
                                            tCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGAc                                              <  1:3541538/93‑1 (MQ=255)
                                              ccAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGc                                            >  2:1315554/1‑93 (MQ=255)
                                              ccAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGc                                            <  1:3267607/93‑1 (MQ=255)
                                                agagTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCa                                          <  2:6287627/93‑1 (MQ=255)
                                                 gagTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAt                                         >  1:1739617/1‑93 (MQ=255)
                                                 gagTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAt                                         <  1:4481253/93‑1 (MQ=255)
                                                 gagTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAt                                         <  2:2948032/93‑1 (MQ=255)
                                                 gagTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAt                                         >  1:1005842/1‑93 (MQ=255)
                                                 gagTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAt                                         >  1:5647433/1‑93 (MQ=255)
                                                  agTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAtt                                        >  1:1547829/1‑93 (MQ=255)
                                                   gTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTa                                       <  2:1927326/93‑1 (MQ=255)
                                                   gTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTa                                       >  1:5344833/1‑93 (MQ=255)
                                                      attaTTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGt                                    <  1:1496585/90‑1 (MQ=255)
                                                      aTGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGt                                    <  2:370623/93‑1 (MQ=255)
                                                      aTGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGt                                    >  1:5130450/1‑93 (MQ=255)
                                                       tGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTg                                   <  1:898045/93‑1 (MQ=255)
                                                         aTTGGCGCAGACTGGTTTACTGACATCATTGTCGGTTCGATGACTGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGAt                                 <  2:5758945/93‑1 (MQ=255)
                                                         aTTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGAt                                 <  2:4569286/93‑1 (MQ=255)
                                                           tGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCg                               <  2:5160414/93‑1 (MQ=255)
                                                            tgcgcACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGa                              <  2:3258558/92‑1 (MQ=255)
                                                            ggCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGa                              <  1:3833886/93‑1 (MQ=255)
                                                            ggCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGa                              <  2:159562/93‑1 (MQ=255)
                                                            ggCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGa                              <  2:4112656/93‑1 (MQ=255)
                                                             gcgcACACTGGTTTACTGACATCATTGTCGGTTCGATGACCTTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGAt                             <  1:5507504/93‑1 (MQ=255)
                                                             gcgcACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGAt                             <  1:6065737/93‑1 (MQ=255)
                                                             gcgcACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGAt                             <  2:78746/93‑1 (MQ=255)
                                                               gcACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTa                           <  1:3089739/93‑1 (MQ=255)
                                                               gcACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTa                           <  2:4136191/93‑1 (MQ=255)
                                                                cacaCTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTaa                          <  1:835117/93‑1 (MQ=255)
                                                                cacaCTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGGGCTGCTGACGCCATTAAGTGATCGATTaa                          >  1:6416361/1‑93 (MQ=255)
                                                                 acacTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAAt                         >  1:179580/1‑93 (MQ=255)
                                                                 acacTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAAt                         >  2:1112268/1‑93 (MQ=255)
                                                                  cacTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATc                        >  1:1952513/1‑93 (MQ=255)
                                                                   acTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCa                       >  2:6496921/1‑93 (MQ=255)
                                                                   acTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCa                       <  2:5942859/93‑1 (MQ=255)
                                                                   acTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCa                       >  1:1434789/1‑93 (MQ=255)
                                                                       gTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtt                   <  2:818187/93‑1 (MQ=255)
                                                                       gTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtt                   <  1:5465463/93‑1 (MQ=255)
                                                                       gTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtt                   <  2:5608877/93‑1 (MQ=255)
                                                                       gTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGGTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtt                   >  1:1318082/1‑93 (MQ=255)
                                                                        tttACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCTGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCttt                  >  2:4676879/1‑93 (MQ=255)
                                                                         ttACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtttt                 <  2:3546022/93‑1 (MQ=255)
                                                                         ttACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtttt                 >  2:5328610/1‑93 (MQ=255)
                                                                         ttACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtttt                 <  2:4898179/93‑1 (MQ=255)
                                                                         ttACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCCGACGCCATTAAGTGATCGATTAATCACCtttt                 >  2:3738364/1‑93 (MQ=255)
                                                                            cTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTg              <  2:4185715/93‑1 (MQ=255)
                                                                              gACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGAc            >  2:6453099/1‑93 (MQ=255)
                                                                               aCATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACa           >  2:1732221/1‑93 (MQ=255)
                                                                               aCATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACa           <  2:6109735/93‑1 (MQ=255)
                                                                               aCATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACa           >  1:5073901/1‑93 (MQ=255)
                                                                                catcatTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACaa          >  2:5833781/1‑93 (MQ=255)
                                                                                 atcatTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACaaa         <  1:420848/93‑1 (MQ=255)
                                                                                  tcatTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAAt        >  2:5220612/1‑93 (MQ=255)
                                                                                  tcatTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAAt        <  1:314753/93‑1 (MQ=255)
                                                                                  tcatTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAAt        >  2:284295/1‑93 (MQ=255)
                                                                                     ttGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCAc     <  1:4384317/93‑1 (MQ=255)
                                                                                      tGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACt    >  1:3493982/1‑93 (MQ=255)
                                                                                       gTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTa   <  2:461926/93‑1 (MQ=255)
                                                                                            |                                                                                        
GTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTAC  >  NC_000913/2267375‑2267555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGA  >  NC_000913/2267374‑2267560
                                                                                             |                                                                                             
AGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGT                                                                                               <  SRR4302547.5952279/94‑1 (MQ=60)
 GTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGGCATCATTGTCGGCT                                                                                              <  SRR4302547.2406834/94‑1 (MQ=60)
 GTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTT                                                                                              <  SRR4302547.6581862/94‑1 (MQ=60)
  TTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTC                                                                                             <  SRR4302547.2113505/94‑1 (MQ=60)
  TTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTC                                                                                             <  SRR4302547.1984338/94‑1 (MQ=60)
   TGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCG                                                                                            <  SRR4302547.3472958/94‑1 (MQ=60)
   TGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCG                                                                                            >  SRR4302547.2834042/1‑94 (MQ=60)
   TGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCG                                                                                            >  SRR4302547.5325846/1‑94 (MQ=60)
    GCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGA                                                                                           >  SRR4302547.1900746/1‑94 (MQ=60)
     CAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGAT                                                                                          <  SRR4302547.529189/94‑1 (MQ=60)
     CAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGAT                                                                                          >  SRR4302547.479994/1‑94 (MQ=60)
      AGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATG                                                                                         >  SRR4302547.6245307/1‑94 (MQ=60)
      AGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATG                                                                                         <  SRR4302547.2649726/94‑1 (MQ=60)
      AGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATG                                                                                         <  SRR4302547.4610962/94‑1 (MQ=60)
       GGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGA                                                                                        >  SRR4302547.447255/1‑94 (MQ=60)
       GGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGA                                                                                        <  SRR4302547.904045/94‑1 (MQ=60)
       GGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGA                                                                                        <  SRR4302547.5194315/94‑1 (MQ=60)
        GCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGAC                                                                                       >  SRR4302547.6049866/1‑94 (MQ=60)
         CCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACC                                                                                      >  SRR4302547.5322004/1‑94 (MQ=60)
         CCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACC                                                                                      <  SRR4302547.2121624/94‑1 (MQ=60)
         CCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGGAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGAAC                                                                                      >  SRR4302547.5707770/1‑94 (MQ=60)
         CCTTATCCCCCTTATTATTTTTGTGGTTTTTGCATTTTCCAGAGGAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGAAGACC                                                                                      >  SRR4302547.1795771/1‑94 (MQ=60)
          CTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCG                                                                                     <  SRR4302547.1788009/94‑1 (MQ=60)
          CTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCG                                                                                     >  SRR4302547.1050697/1‑94 (MQ=60)
           TTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGT                                                                                    <  SRR4302547.5323639/94‑1 (MQ=60)
           TTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGT                                                                                    >  SRR4302547.5805668/1‑94 (MQ=60)
            TATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTG                                                                                   <  SRR4302547.1013087/94‑1 (MQ=60)
              TCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGAT                                                                                 >  SRR4302547.4836374/1‑94 (MQ=60)
              TCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGAT                                                                                 <  SRR4302547.1126643/94‑1 (MQ=60)
              TCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGAT                                                                                 >  SRR4302547.2735380/1‑94 (MQ=60)
               CGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATA                                                                                >  SRR4302547.2147194/1‑94 (MQ=60)
               CGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATA                                                                                <  SRR4302547.4996348/94‑1 (MQ=60)
                GCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATAT                                                                               >  SRR4302547.143061/1‑94 (MQ=60)
                GCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATAT                                                                               <  SRR4302547.4574771/94‑1 (MQ=60)
                GCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATAT                                                                               >  SRR4302547.3467214/1‑94 (MQ=60)
                GCCCTTATTATTTTTGTGGCTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATAT                                                                               >  SRR4302547.6569411/1‑94 (MQ=60)
                 CCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATT                                                                              >  SRR4302547.2019963/1‑94 (MQ=60)
                 CCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATT                                                                              >  SRR4302547.5311024/1‑94 (MQ=60)
                 CCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATT                                                                              >  SRR4302547.1777424/1‑94 (MQ=60)
                  CCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTT                                                                             >  SRR4302547.3685687/1‑94 (MQ=60)
                  CCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTG                                                                             >  SRR4302547.1421290/1‑94 (MQ=60)
                  CCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTG                                                                             >  SRR4302547.4648337/1‑94 (MQ=60)
                  CCTTATTATTTTTGTGGTTTCTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTG                                                                             <  SRR4302547.3571772/94‑1 (MQ=60)
                    TTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGAT                                                                           >  SRR4302547.6596330/1‑94 (MQ=60)
                    TTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGAT                                                                           <  SRR4302547.5431628/94‑1 (MQ=60)
                    TTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGAT                                                                           >  SRR4302547.400574/1‑94 (MQ=60)
                     TATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATC                                                                          >  SRR4302547.679885/1‑94 (MQ=60)
                     TATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATC                                                                          <  SRR4302547.1498789/94‑1 (MQ=60)
                      ATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCG                                                                         <  SRR4302547.1357326/94‑1 (MQ=60)
                       TTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGG                                                                        <  SRR4302547.2521134/94‑1 (MQ=60)
                          TTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTT                                                                     >  SRR4302547.5314101/1‑94 (MQ=60)
                           TTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTG                                                                    <  SRR4302547.3168587/94‑1 (MQ=60)
                           TTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTG                                                                    <  SRR4302547.73993/94‑1 (MQ=60)
                           TTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTG                                                                    >  SRR4302547.5573103/1‑94 (MQ=60)
                             TTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCC                                                                  <  SRR4302547.6236427/94‑1 (MQ=60)
                              TGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCC                                                                 <  SRR4302547.1742120/94‑1 (MQ=60)
                              TGTGGTTTTTGCATTTCCCAGAGTAATGATTAGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGGTATTGATCGGTTTTCCC                                                                 >  SRR4302547.1389360/1‑94 (MQ=60)
                               GTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCT                                                                <  SRR4302547.713809/94‑1 (MQ=60)
                               GTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCT                                                                <  SRR4302547.763526/94‑1 (MQ=60)
                                TGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTG                                                               <  SRR4302547.156689/94‑1 (MQ=60)
                                 GGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGG                                                              >  SRR4302547.1052070/1‑94 (MQ=60)
                                  GTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGG                                                             >  SRR4302547.2980131/1‑94 (MQ=60)
                                    TTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTG                                                           <  SRR4302547.3689363/94‑1 (MQ=60)
                                     TTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGCCATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGT                                                          <  SRR4302547.6689411/94‑1 (MQ=60)
                                     TTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGT                                                          >  SRR4302547.4736083/1‑94 (MQ=60)
                                      TTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTT                                                         >  SRR4302547.5467941/1‑94 (MQ=60)
                                       TGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTG                                                        <  SRR4302547.3480967/94‑1 (MQ=60)
                                       TGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTG                                                        >  SRR4302547.1785063/1‑94 (MQ=60)
                                        GCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGC                                                       >  SRR4302547.3867245/1‑94 (MQ=60)
                                        GCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGC                                                       <  SRR4302547.3134948/94‑1 (MQ=60)
                                         GATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCT                                                      <  SRR4302547.2229201/94‑1 (MQ=60)
                                         CATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCT                                                      <  SRR4302547.6433837/94‑1 (MQ=60)
                                          ATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTG                                                     >  SRR4302547.3023016/1‑94 (MQ=60)
                                          ATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTG                                                     <  SRR4302547.2813259/94‑1 (MQ=60)
                                          ATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTG                                                     >  SRR4302547.6685806/1‑94 (MQ=60)
                                            TTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGAC                                                   <  SRR4302547.3442951/94‑1 (MQ=60)
                                             TCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACG                                                  <  SRR4302547.3613829/94‑1 (MQ=60)
                                               CCAGAGTAATGATTGGCGTGCACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCC                                                <  SRR4302547.3359457/94‑1 (MQ=60)
                                               CCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCC                                                <  SRR4302547.3332986/94‑1 (MQ=60)
                                                CAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCA                                               <  SRR4302547.2987275/94‑1 (MQ=60)
                                                 AGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAT                                              >  SRR4302547.5763998/1‑94 (MQ=60)
                                                 AGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAT                                              >  SRR4302547.1025475/1‑94 (MQ=60)
                                                 AGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCAT                                              >  SRR4302547.1773832/1‑94 (MQ=60)
                                                  GAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATT                                             <  SRR4302547.4573830/94‑1 (MQ=60)
                                                  GAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATT                                             >  SRR4302547.6458718/1‑94 (MQ=60)
                                                  GAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATT                                             >  SRR4302547.1578645/1‑94 (MQ=60)
                                                   AGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTA                                            >  SRR4302547.6208569/1‑94 (MQ=60)
                                                   AGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTA                                            >  SRR4302547.5455015/1‑94 (MQ=60)
                                                     TAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAG                                          >  SRR4302547.2601065/1‑94 (MQ=60)
                                                     TAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAG                                          <  SRR4302547.6525231/94‑1 (MQ=60)
                                                      AATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGT                                         >  SRR4302547.5236159/1‑94 (MQ=60)
                                                       ATTATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTG                                        <  SRR4302547.1526440/94‑1 (MQ=60)
                                                       ATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTG                                        <  SRR4302547.2061435/94‑1 (MQ=60)
                                                        TGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGA                                       <  SRR4302547.3306116/94‑1 (MQ=60)
                                                        TGATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGA                                       <  SRR4302547.915312/94‑1 (MQ=60)
                                                          ATTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATC                                     <  SRR4302547.5445207/94‑1 (MQ=60)
                                                           TTGGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCG                                    <  SRR4302547.3924878/94‑1 (MQ=60)
                                                             GGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGAT                                  <  SRR4302547.3911977/94‑1 (MQ=60)
                                                             GGCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGAT                                  >  SRR4302547.6093244/1‑94 (MQ=60)
                                                              GCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCTTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATT                                 <  SRR4302547.5621220/94‑1 (MQ=60)
                                                              GCGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATT                                 <  SRR4302547.6191554/94‑1 (MQ=60)
                                                               CGCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTA                                <  SRR4302547.4746497/94‑1 (MQ=60)
                                                                GCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAA                               <  SRR4302547.3151024/94‑1 (MQ=60)
                                                                GCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAA                               >  SRR4302547.227768/1‑94 (MQ=60)
                                                                GCACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGGGCTGCTGACGCCATTAAGTGATCGATTAA                               >  SRR4302547.6550151/1‑94 (MQ=60)
                                                                 CACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAAT                              >  SRR4302547.182949/1‑94 (MQ=60)
                                                                 CACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAAT                              <  SRR4302547.851058/94‑1 (MQ=60)
                                                                  ACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATC                             >  SRR4302547.1991239/1‑94 (MQ=60)
                                                                  ACACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATC                             >  SRR4302547.4985515/1‑94 (MQ=60)
                                                                   CACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCA                            >  SRR4302547.1463474/1‑94 (MQ=60)
                                                                    ACTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCAC                           >  SRR4302547.5796500/1‑94 (MQ=60)
                                                                     CTGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACC                          >  SRR4302547.439871/1‑94 (MQ=60)
                                                                      TGGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCT                         >  SRR4302547.945967/1‑94 (MQ=60)
                                                                       GGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTT                        <  SRR4302547.5076729/94‑1 (MQ=60)
                                                                       GGTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGGTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTT                        >  SRR4302547.1344572/1‑94 (MQ=60)
                                                                        GTTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTT                       <  SRR4302547.5578362/94‑1 (MQ=60)
                                                                         TTTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTT                      >  SRR4302547.5583374/1‑94 (MQ=60)
                                                                          TTACTGACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTT                     <  SRR4302547.2728172/94‑1 (MQ=60)
                                                                               GACATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACA                >  SRR4302547.5178581/1‑94 (MQ=60)
                                                                                 CATAATTGTCGCCTCGATGACCGTAATATTGATCGGTTTGCCTGGGGTGTTGCTGCCGCCATTAGGTGATCGA‑TAATCACCTTTTTTGACAAAT             <  SRR4302547.5532910/94‑1 (MQ=52)
                                                                                  ATCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAAT             <  SRR4302547.428646/94‑1 (MQ=60)
                                                                                   TCATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATC            <  SRR4302547.320621/94‑1 (MQ=60)
                                                                                    CATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCA           <  SRR4302547.3816420/94‑1 (MQ=60)
                                                                                    CATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCA           <  SRR4302547.850901/94‑1 (MQ=60)
                                                                                     ATTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCAC          >  SRR4302547.3072825/1‑94 (MQ=60)
                                                                                      TTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACT         >  SRR4302547.3565281/1‑94 (MQ=60)
                                                                                      TTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACT         <  SRR4302547.4274090/94‑1 (MQ=60)
                                                                                      TTGTCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACT         <  SRR4302547.4474511/94‑1 (MQ=60)
                                                                                         TCGGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACC      <  SRR4302547.4535986/94‑1 (MQ=60)
                                                                                           GGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTTATCACATTTTTTGACAAATCACTACCAG    >  SRR4302547.6461580/1‑94 (MQ=60)
                                                                                           GGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAG    <  SRR4302547.3537322/94‑1 (MQ=60)
                                                                                           GGCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAG    >  SRR4302547.3305316/1‑94 (MQ=60)
                                                                                            GCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGG   >  SRR4302547.318355/1‑94 (MQ=60)
                                                                                            GCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGG   <  SRR4302547.3759860/94‑1 (MQ=60)
                                                                                            GCTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGG   <  SRR4302547.815121/94‑1 (MQ=60)
                                                                                             CTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGA  <  SRR4302547.5813687/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGA  >  NC_000913/2267374‑2267560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑