Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,273 A→G L111P (CTG→CCG)  b2335 ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2730AG83.4% 562.1 / 104.6 235L111P (CTG→CCG) b2335putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base A (26/13);  new base G (92/104);  total (118/117)
Fisher's exact test for biased strand distribution p-value = 3.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 249 total aligned reads displayed.

TGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT  >  NC_000913/2449183‑2449365
                                                                                          |                                                                                            
tgtgGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGc                                                                                            >  2:5206105/1‑93 (MQ=255)
   ggCTGTTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCg                                                                                         <  1:3195077/93‑1 (MQ=255)
   ggCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTTACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATgcccggcccg                                                                                         >  1:6648327/1‑93 (MQ=255)
   ggCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATgcccggcccg                                                                                         >  2:4264816/1‑93 (MQ=255)
   ggCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATgcccggcccg                                                                                         >  1:2535933/1‑93 (MQ=255)
     cTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGt                                                                                       >  1:4639351/1‑93 (MQ=255)
      tGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTg                                                                                      >  2:3328126/1‑93 (MQ=255)
      tGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTg                                                                                      >  1:2856274/1‑93 (MQ=255)
      tGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTg                                                                                      <  1:970485/93‑1 (MQ=255)
       ggTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGa                                                                                     >  2:2970451/1‑93 (MQ=255)
       ggTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGa                                                                                     >  1:1027987/1‑93 (MQ=255)
        gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGat                                                                                    >  2:378238/1‑93 (MQ=255)
          tATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGatat                                                                                  >  1:1517052/1‑93 (MQ=255)
           aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATAtt                                                                                 >  2:3653361/1‑93 (MQ=255)
            ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTg                                                                                <  2:1061426/93‑1 (MQ=255)
              tatatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGt                                                                              <  1:2098218/92‑1 (MQ=255)
              gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGt                                                                              >  1:732751/1‑93 (MQ=255)
              gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGt                                                                              >  2:2758363/1‑93 (MQ=255)
                tatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTAc                                                                            <  1:4915797/93‑1 (MQ=255)
                tatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTAc                                                                            >  1:4359062/1‑93 (MQ=255)
                 atTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACt                                                                           >  1:1293196/1‑93 (MQ=255)
                     aaTGTCGTCCAGCTATTTTCACCTACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGt                                                                       <  1:2157396/93‑1 (MQ=255)
                      aTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTa                                                                      <  2:4765552/93‑1 (MQ=255)
                      aTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTa                                                                      <  1:4131063/93‑1 (MQ=255)
                       tGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTAc                                                                     >  2:6486261/1‑93 (MQ=255)
                       tGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTAc                                                                     <  1:6436462/93‑1 (MQ=255)
                        ggCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTAcc                                                                    <  2:4350607/93‑1 (MQ=255)
                         gCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCg                                                                   <  2:4902204/93‑1 (MQ=255)
                            tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGttt                                                                >  1:5762382/1‑93 (MQ=255)
                            tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGttt                                                                <  2:2833817/93‑1 (MQ=255)
                               aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGc                                                             <  2:5281799/93‑1 (MQ=255)
                               aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGc                                                             <  2:574529/93‑1 (MQ=255)
                               aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGc                                                             >  2:4820859/1‑93 (MQ=255)
                               aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGc                                                             >  1:5186517/1‑93 (MQ=255)
                                gCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGcc                                                            >  2:2217618/1‑93 (MQ=255)
                                gCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGcc                                                            >  2:5056687/1‑93 (MQ=255)
                                 cTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCg                                                           >  2:6003811/1‑93 (MQ=255)
                                  tATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGt                                                          <  2:4403431/93‑1 (MQ=255)
                                  tATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGt                                                          <  1:3302409/93‑1 (MQ=255)
                                   aTTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGtt                                                         <  2:5878985/93‑1 (MQ=255)
                                    ttttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTa                                                        >  1:6624034/1‑93 (MQ=255)
                                     tttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTaa                                                       <  1:5118347/93‑1 (MQ=255)
                                     tttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTaa                                                       >  2:89058/1‑93 (MQ=255)
                                     tttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTaa                                                       <  1:648821/93‑1 (MQ=255)
                                      ttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAAt                                                      <  2:1513307/93‑1 (MQ=255)
                                      ttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAAt                                                      <  1:38410/93‑1 (MQ=255)
                                       tCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATc                                                     <  2:3896933/93‑1 (MQ=255)
                                       tCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATc                                                     <  2:5793126/93‑1 (MQ=255)
                                        cACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCg                                                    <  1:1783557/93‑1 (MQ=255)
                                         aCCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGt                                                   <  1:896968/93‑1 (MQ=255)
                                          ccGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGtt                                                  >  1:2120425/1‑93 (MQ=255)
                                          ccGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGtt                                                  <  2:5598013/93‑1 (MQ=255)
                                           cGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTg                                                 >  1:6623116/1‑93 (MQ=255)
                                           cGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTg                                                 >  2:1393264/1‑93 (MQ=255)
                                            gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGa                                                >  2:4488360/1‑93 (MQ=255)
                                             aCGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAg                                               <  2:6341480/93‑1 (MQ=255)
                                              cGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAgg                                              >  2:143773/1‑93 (MQ=255)
                                               ggCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGt                                             <  2:2673712/93‑1 (MQ=255)
                                               ggCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGt                                             <  2:951914/93‑1 (MQ=255)
                                               ggCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGt                                             <  1:1478552/93‑1 (MQ=255)
                                               ggCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGt                                             <  1:2371659/93‑1 (MQ=255)
                                                  aaaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAtt                                          >  1:4079961/1‑93 (MQ=255)
                                                   aataaTGAGTTATCGGCGCTATTTTCTATCCGACTGCCCGGCCCGGTGATATTGGTACTGAGGACCGTTTCGCCGTTAATCGTTGAGGGATTg                                         >  1:2036060/4‑93 (MQ=255)
                                                   aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                         >  2:3589142/1‑93 (MQ=255)
                                                   aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                         >  1:2974634/1‑93 (MQ=255)
                                                   aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                         <  2:322209/93‑1 (MQ=255)
                                                      aaTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGccc                                      <  2:2115097/93‑1 (MQ=255)
                                                      aaTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGccc                                      <  1:3718807/93‑1 (MQ=255)
                                                       aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                     <  2:238555/93‑1 (MQ=255)
                                                       aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                     >  2:711692/1‑93 (MQ=255)
                                                        tGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCtt                                    >  2:333509/1‑93 (MQ=255)
                                                        tGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCtt                                    >  1:5139395/1‑93 (MQ=255)
                                                           gTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAg                                 <  1:25034/93‑1 (MQ=255)
                                                           gTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAg                                 <  1:1698446/93‑1 (MQ=255)
                                                           gTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAg                                 <  2:4171608/93‑1 (MQ=255)
                                                           gTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAg                                 >  1:4226576/1‑93 (MQ=255)
                                                            ttATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGt                                >  2:1908876/1‑93 (MQ=255)
                                                             tATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                               <  2:2472999/93‑1 (MQ=255)
                                                              aTCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTg                              <  1:3639268/93‑1 (MQ=255)
                                                              aTCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTg                              >  1:5702945/1‑93 (MQ=255)
                                                               tCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGc                             <  2:1852214/93‑1 (MQ=255)
                                                                 ggCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAt                           <  1:5583013/93‑1 (MQ=255)
                                                                  gcgcTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                          >  2:1310328/1‑93 (MQ=255)
                                                                    gcTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCg                        >  1:3526019/1‑93 (MQ=255)
                                                                     cTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGc                       >  1:4553514/1‑93 (MQ=255)
                                                                     cTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGc                       >  1:563414/1‑93 (MQ=255)
                                                                        ttttCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAg                    <  2:5412220/93‑1 (MQ=255)
                                                                         tttCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                   <  1:1122986/93‑1 (MQ=255)
                                                                          ttCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTc                  <  1:2989317/93‑1 (MQ=255)
                                                                           tCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCg                 >  1:3107528/1‑93 (MQ=255)
                                                                             tATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGtt               >  2:112039/1‑93 (MQ=255)
                                                                              aTCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa              <  2:5579489/93‑1 (MQ=255)
                                                                              aTCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa              <  2:6379210/93‑1 (MQ=255)
                                                                              aTCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa              <  1:5966070/93‑1 (MQ=255)
                                                                                   aaTGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAg         >  1:2341440/1‑93 (MQ=255)
                                                                                   aaTGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATACGCAAGTCGTTAGCAAg         >  1:1900133/1‑93 (MQ=255)
                                                                                   aaTGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAg         >  1:2769000/1‑93 (MQ=255)
                                                                                    aTGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGa        <  1:4803377/93‑1 (MQ=255)
                                                                                      gcccggcccgGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACt      <  2:3073601/93‑1 (MQ=255)
                                                                                          aGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt  >  1:843180/1‑93 (MQ=255)
                                                                                          |                                                                                            
TGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT  >  NC_000913/2449183‑2449365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG  >  NC_000913/2449180‑2449366
                                                                                             |                                                                                             
AACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCG                                                                                               >  SRR4302547.292198/1‑94 (MQ=60)
AACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCG                                                                                               <  SRR4302547.4156288/94‑1 (MQ=60)
AACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCA                                                                                               <  SRR4302547.5123428/94‑1 (MQ=60)
 ACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGG                                                                                              >  SRR4302547.3392605/1‑94 (MQ=60)
 ACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGG                                                                                              <  SRR4302547.2483253/94‑1 (MQ=60)
  ATGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTAGTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGC                                                                                             <  SRR4302547.1900952/94‑1 (MQ=60)
    GTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCC                                                                                           >  SRR4302547.3038579/1‑94 (MQ=60)
    GTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCC                                                                                           >  SRR4302547.421521/1‑94 (MQ=60)
     TGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTTACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCG                                                                                          >  SRR4302547.6786817/1‑94 (MQ=60)
     TGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCG                                                                                          >  SRR4302547.2587174/1‑94 (MQ=60)
      GGCTGTTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGG                                                                                         <  SRR4302547.3258742/94‑1 (MQ=60)
       GCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGT                                                                                        >  SRR4302547.4735442/1‑94 (MQ=60)
       GCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGT                                                                                        <  SRR4302547.473379/94‑1 (MQ=60)
        CTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTG                                                                                       >  SRR4302547.2913346/1‑94 (MQ=60)
         TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGA                                                                                      >  SRR4302547.6778289/1‑94 (MQ=60)
         TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGA                                                                                      >  SRR4302547.5849347/1‑94 (MQ=60)
         TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGA                                                                                      <  SRR4302547.989335/94‑1 (MQ=60)
         TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGA                                                                                      >  SRR4302547.1052474/1‑94 (MQ=60)
         TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGA                                                                                      >  SRR4302547.1048184/1‑94 (MQ=60)
          GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGAT                                                                                     <  SRR4302547.6721002/94‑1 (MQ=60)
            TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATAT                                                                                   >  SRR4302547.417028/1‑94 (MQ=60)
            TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATAT                                                                                   <  SRR4302547.2402500/94‑1 (MQ=60)
            TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATAT                                                                                   >  SRR4302547.1547271/1‑94 (MQ=60)
             TATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATT                                                                                  >  SRR4302547.3045812/1‑94 (MQ=60)
              ATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTG                                                                                 >  SRR4302547.501630/1‑94 (MQ=60)
              ATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTG                                                                                 >  SRR4302547.2394278/1‑94 (MQ=60)
                TGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGT                                                                               >  SRR4302547.746439/1‑94 (MQ=60)
                TGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGT                                                                               <  SRR4302547.3860389/94‑1 (MQ=60)
                TGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGT                                                                               <  SRR4302547.5758253/94‑1 (MQ=60)
                 TATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTA                                                                              <  SRR4302547.2140043/94‑1 (MQ=60)
                  ATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTAC                                                                             >  SRR4302547.4448594/1‑94 (MQ=60)
                   TATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACT                                                                            <  SRR4302547.5017604/94‑1 (MQ=60)
                   TATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACT                                                                            <  SRR4302547.2422414/94‑1 (MQ=60)
                   TATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACT                                                                            >  SRR4302547.1319222/1‑94 (MQ=60)
                    ATTAAATGGCGTCCAGCTATGTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAAAGCCCAGCCCGGTAAAATTGGTACTG                                                                           <  SRR4302547.5862904/94‑1 (MQ=60)
                     TTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGA                                                                          <  SRR4302547.3733046/94‑1 (MQ=60)
                      TAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAG                                                                         >  SRR4302547.5854052/1‑94 (MQ=60)
                        AATGTCGTCCAGCTATTTTCACCTACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTA                                                                       <  SRR4302547.2200505/94‑1 (MQ=60)
                        AATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTA                                                                       <  SRR4302547.3186604/94‑1 (MQ=60)
                         ATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTAC                                                                      <  SRR4302547.1672088/94‑1 (MQ=60)
                         ATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTAC                                                                      <  SRR4302547.4215112/94‑1 (MQ=60)
                          TGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACC                                                                     <  SRR4302547.6570610/94‑1 (MQ=60)
                           GGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCG                                                                    <  SRR4302547.6290725/94‑1 (MQ=60)
                           GGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCG                                                                    >  SRR4302547.3454385/1‑94 (MQ=60)
                              GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTT                                                                 >  SRR4302547.5881556/1‑94 (MQ=60)
                                CCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCG                                                               <  SRR4302547.4282134/94‑1 (MQ=60)
                                 CAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGC                                                              >  SRR4302547.5293261/1‑94 (MQ=60)
                                  AGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCC                                                             >  SRR4302547.4534805/1‑94 (MQ=60)
                                   GCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCG                                                            <  SRR4302547.4713916/94‑1 (MQ=60)
                                    CTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGT                                                           >  SRR4302547.6761034/1‑94 (MQ=60)
                                     TATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTT                                                          <  SRR4302547.3368894/94‑1 (MQ=60)
                                      ATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTA                                                         >  SRR4302547.6761922/1‑94 (MQ=60)
                                        TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                       <  SRR4302547.4818938/94‑1 (MQ=60)
                                        TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                       <  SRR4302547.660778/94‑1 (MQ=60)
                                        TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                       <  SRR4302547.5223803/94‑1 (MQ=60)
                                         TTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATC                                                      <  SRR4302547.39128/94‑1 (MQ=60)
                                          TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCG                                                     <  SRR4302547.572402/94‑1 (MQ=60)
                                           CACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGT                                                    <  SRR4302547.1818884/94‑1 (MQ=60)
                                            ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                   <  SRR4302547.5224363/94‑1 (MQ=60)
                                            ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                   <  SRR4302547.914216/94‑1 (MQ=60)
                                            ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                   >  SRR4302547.2162688/1‑94 (MQ=60)
                                             CCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTG                                                  >  SRR4302547.6760980/1‑94 (MQ=60)
                                              CGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGA                                                 >  SRR4302547.1848447/1‑94 (MQ=60)
                                               GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                <  SRR4302547.1228206/94‑1 (MQ=60)
                                                ACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGG                                               <  SRR4302547.5248835/94‑1 (MQ=60)
                                                 CGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGT                                              >  SRR4302547.3199346/1‑94 (MQ=60)
                                                 CGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGT                                              <  SRR4302547.3017301/94‑1 (MQ=60)
                                                 CGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGT                                              >  SRR4302547.3053922/1‑94 (MQ=60)
                                                 CGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGT                                              <  SRR4302547.785822/94‑1 (MQ=60)
                                                  GGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTA                                             <  SRR4302547.1508078/94‑1 (MQ=60)
                                                  GGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTA                                             <  SRR4302547.2419725/94‑1 (MQ=60)
                                                   GCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAT                                            >  SRR4302547.2090475/1‑94 (MQ=60)
                                                    CAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATT                                           >  SRR4302547.1779013/1‑94 (MQ=60)
                                                    CAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATT                                           >  SRR4302547.4594658/1‑94 (MQ=60)
                                                    CAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATT                                           >  SRR4302547.4162873/1‑94 (MQ=60)
                                                     AAATAATGAGTTATCGGCGCTATTTTCTATCCGACTGCCCGGCCCGGTGATATTGGTACTGAGGACCGTTTCGCCGTTAATCGTTGAGGGATTG                                          >  SRR4302547.2076639/1‑94 (MQ=60)
                                                     AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTG                                          >  SRR4302547.3033829/1‑94 (MQ=60)
                                                     AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTG                                          >  SRR4302547.255640/1‑94 (MQ=60)
                                                      AACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGCGATATTGGTACTGAGTACCGGTTCGCCGTTAATCGCTGACGTATTGC                                         >  SRR4302547.2660061/1‑94 (MQ=60)
                                                        CAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCC                                       <  SRR4302547.165530/94‑1 (MQ=60)
                                                         AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                      >  SRR4302547.5166840/1‑94 (MQ=60)
                                                         AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                      <  SRR4302547.5014289/94‑1 (MQ=60)
                                                         AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                      >  SRR4302547.257523/1‑94 (MQ=60)
                                                         AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                      <  SRR4302547.5715358/94‑1 (MQ=60)
                                                         AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                      <  SRR4302547.1875941/94‑1 (MQ=60)
                                                         AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                      <  SRR4302547.3794719/94‑1 (MQ=60)
                                                          ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTT                                     >  SRR4302547.5245272/1‑94 (MQ=60)
                                                          ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTT                                     >  SRR4302547.5391618/1‑94 (MQ=60)
                                                           TGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTA                                    <  SRR4302547.2077389/94‑1 (MQ=60)
                                                           TGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTA                                    <  SRR4302547.524680/94‑1 (MQ=60)
                                                           TGAGTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTA                                    >  SRR4302547.995982/1‑94 (MQ=60)
                                                             AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                  >  SRR4302547.4312950/1‑94 (MQ=60)
                                                              GTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGT                                 <  SRR4302547.25496/94‑1 (MQ=60)
                                                              GTTATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGT                                 <  SRR4302547.1731836/94‑1 (MQ=60)
                                                                TATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTG                               <  SRR4302547.218047/94‑1 (MQ=60)
                                                                TATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTG                               >  SRR4302547.5820747/1‑94 (MQ=60)
                                                                 ATCGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGC                              <  SRR4302547.3713491/94‑1 (MQ=60)
                                                                   CGGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAT                            <  SRR4302547.3788454/94‑1 (MQ=60)
                                                                    GGCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATC                           <  SRR4302547.5698167/94‑1 (MQ=60)
                                                                     GCGCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                          <  SRR4302547.3327043/94‑1 (MQ=60)
                                                                      CGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCG                         >  SRR4302547.3598010/1‑94 (MQ=60)
                                                                       GCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGC                        >  SRR4302547.4647701/1‑94 (MQ=60)
                                                                       GCTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGC                        >  SRR4302547.573558/1‑94 (MQ=60)
                                                                        CTATTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCA                       <  SRR4302547.768214/94‑1 (MQ=60)
                                                                        CTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCA                       >  SRR4302547.2145659/1‑94 (MQ=60)
                                                                           TTTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGT                    <  SRR4302547.6774406/94‑1 (MQ=60)
                                                                            TTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTC                   <  SRR4302547.1145638/94‑1 (MQ=60)
                                                                            TTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTC                   <  SRR4302547.1480112/94‑1 (MQ=60)
                                                                            TTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTC                   <  SRR4302547.5970012/94‑1 (MQ=60)
                                                                            TTTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTC                   <  SRR4302547.3630367/94‑1 (MQ=60)
                                                                             TTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCG                  <  SRR4302547.1533189/94‑1 (MQ=60)
                                                                             TTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCG                  <  SRR4302547.2839068/94‑1 (MQ=60)
                                                                             TTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCG                  <  SRR4302547.3048791/94‑1 (MQ=60)
                                                                             TTCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCG                  >  SRR4302547.3169113/1‑94 (MQ=60)
                                                                              TCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGT                 >  SRR4302547.740940/1‑94 (MQ=60)
                                                                              TCTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGT                 >  SRR4302547.2008432/1‑94 (MQ=60)
                                                                               CTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                >  SRR4302547.957936/1‑94 (MQ=60)
                                                                               CTATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                <  SRR4302547.6727400/94‑1 (MQ=60)
                                                                               CTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                >  SRR4302547.2164672/1‑94 (MQ=60)
                                                                                TATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGAAAGTCGTTA               <  SRR4302547.6605542/94‑1 (MQ=60)
                                                                                 ATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAG              <  SRR4302547.6089807/94‑1 (MQ=60)
                                                                                 ATCCGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAG              <  SRR4302547.3974145/94‑1 (MQ=60)
                                                                                 ATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAG              >  SRR4302547.6325420/1‑94 (MQ=60)
                                                                                    CGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAA           >  SRR4302547.903009/1‑94 (MQ=60)
                                                                                    CGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAA           >  SRR4302547.4325262/1‑94 (MQ=60)
                                                                                    CGAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTCCCCTTAAGTTGCATCCGCAAGTCGTTAGCAA           >  SRR4302547.4112000/1‑94 (MQ=60)
                                                                                     GAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG          >  SRR4302547.2388895/1‑94 (MQ=60)
                                                                                     GAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG          >  SRR4302547.2291857/1‑94 (MQ=60)
                                                                                     GAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG          >  SRR4302547.1319777/1‑94 (MQ=60)
                                                                                     GAATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATACGCAAGTCGTTAGCAAG          >  SRR4302547.1937726/1‑94 (MQ=60)
                                                                                     GAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG          >  SRR4302547.2824473/1‑94 (MQ=60)
                                                                                       ATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAC        >  SRR4302547.3401826/1‑94 (MQ=60)
                                                                                       ATGCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAC        <  SRR4302547.4902906/94‑1 (MQ=60)
                                                                                         GCCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTG      >  SRR4302547.2866072/1‑94 (MQ=60)
                                                                                          CCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGT     >  SRR4302547.6727910/1‑94 (MQ=60)
                                                                                          CCCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGT     >  SRR4302547.5726705/1‑94 (MQ=60)
                                                                                           CCGGCGCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT    <  SRR4302547.3268394/94‑1 (MQ=60)
                                                                                           CCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT    <  SRR4302547.728975/94‑1 (MQ=60)
                                                                                           CCGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT    >  SRR4302547.3878468/1‑94 (MQ=60)
                                                                                            CGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT   >  SRR4302547.3539001/1‑94 (MQ=60)
                                                                                            CGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT   >  SRR4302547.6502021/1‑94 (MQ=60)
                                                                                            CGGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT   >  SRR4302547.3887420/1‑94 (MQ=60)
                                                                                            CAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT   >  SRR4302547.859276/1‑94 (MQ=60)
                                                                                             GGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG  >  SRR4302547.1446765/1‑94 (MQ=60)
                                                                                             GGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG  <  SRR4302547.701142/94‑1 (MQ=60)
                                                                                             GGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG  <  SRR4302547.5116825/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG  >  NC_000913/2449180‑2449366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑