Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,483,335	(C)_6→7	coding (942/3594 nt)	evgS →	EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,329	1	.	C	85.8%	535.4 / 80.1	169	coding (936/3594 nt)	evgS	EvgS
Reads supporting (aligned to +/- strand): ref base . (10/14); new base C (72/73); total (82/87)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 192 total aligned reads displayed.

GCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAA  >  NC_000913/2483237‑2483418

 ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑c                                                                                          >  2:4734808/1‑93 (MQ=255)

 ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑c                                                                                          >  2:6442535/1‑93 (MQ=255)

 ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑c                                                                                          <  1:2430680/93‑1 (MQ=255)

   tttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑ccc                                                                                        >  2:1242067/1‑93 (MQ=255)

   tttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑ccc                                                                                        >  2:41310/1‑93 (MQ=255)

   tttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑ccc                                                                                        >  1:5547653/1‑93 (MQ=255)

    ttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑cccc                                                                                       <  2:1008416/93‑1 (MQ=255)

      cTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑cccccc                                                                                     <  2:4059246/93‑1 (MQ=255)

        gAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑ccccccct                                                                                   <  2:3720617/93‑3 (MQ=255)

         aaCAAACCATTAGAACTCACTGAACATGAAACACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑cccccccta                                                                                  <  2:3736009/93‑4 (MQ=255)

          aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑ccccccctat                                                                                 >  2:1900894/1‑89 (MQ=255)

          aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑ccccccctat                                                                                 <  1:6285983/93‑5 (MQ=255)

            aaaCCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTc                                                                                <  2:6083938/93‑1 (MQ=255)

             aaCCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCt                                                                               >  1:4159693/1‑93 (MQ=255)

              aCCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTa                                                                              <  1:834939/93‑1 (MQ=255)

              aCCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTa                                                                              <  1:3242257/93‑1 (MQ=255)

              aCCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTa                                                                              >  2:5899226/1‑93 (MQ=255)

               ccATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTAt                                                                             >  2:3472745/1‑93 (MQ=255)

                cATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATg                                                                            >  2:3930080/1‑93 (MQ=255)

                 aTTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGa                                                                           >  2:4846769/1‑93 (MQ=255)

                  ttAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGAc                                                                          >  2:6605016/1‑93 (MQ=255)

                  ttAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGAc                                                                          >  1:5219940/1‑93 (MQ=255)

                    aGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACgg                                                                        <  2:126837/93‑1 (MQ=255)

                     gAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGa                                                                       <  2:1061333/93‑1 (MQ=255)

                       acacACTGGACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATg                                                                     >  1:6540602/4‑93 (MQ=255)

                       aCTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATg                                                                     <  2:2882554/93‑1 (MQ=255)

                       aCTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATg                                                                     >  2:3084314/1‑93 (MQ=255)

                         tCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGaa                                                                   >  2:1849432/1‑93 (MQ=255)

                         tCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGaa                                                                   <  1:1012315/93‑1 (MQ=255)

                         tCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGaa                                                                   <  2:3851550/93‑1 (MQ=255)

                         ccACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGaa                                                                   <  1:118450/92‑1 (MQ=255)

                           aCTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGaaaa                                                                 >  2:2134805/1‑93 (MQ=255)

                            cTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAAt                                                                >  1:5915891/1‑93 (MQ=255)

                             tGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATg                                                               <  2:4004231/93‑1 (MQ=255)

                               aaCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGc                                                             >  1:6244762/1‑93 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCt                                                            >  1:5690944/1‑93 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCt                                                            <  1:601788/93‑1 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCt                                                            <  2:263815/93‑1 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCt                                                            <  1:1697290/93‑1 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCt                                                            <  1:2895579/93‑1 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCt                                                            <  1:1985664/93‑1 (MQ=255)

                                aCATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTc                                                           <  1:6174637/93‑1 (MQ=255)

                                 cATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTc                                                           <  1:636326/93‑1 (MQ=255)

                                 cATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTc                                                           <  2:6038032/93‑1 (MQ=255)

                                 cATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTc                                                           >  1:900287/1‑93 (MQ=255)

                                   tGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCgg                                                         <  2:5451339/93‑1 (MQ=255)

                                   tGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGt                                                        <  1:1797951/93‑1 (MQ=255)

                                    gAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGt                                                        >  2:5815173/1‑93 (MQ=255)

                                    gAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGt                                                        <  1:2974936/93‑1 (MQ=255)

                                     aaaaaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGtt                                                       >  2:5701242/1‑93 (MQ=255)

                                     aaaaaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGtt                                                       >  2:1406174/1‑93 (MQ=255)

                                      aaaaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTc                                                      <  2:2731988/93‑1 (MQ=255)

                                       aaaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCg                                                     <  2:2683199/93‑1 (MQ=255)

                                         aCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCggg                                                   >  2:2782244/1‑93 (MQ=255)

                                          cAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgggg                                                  <  2:4046567/93‑1 (MQ=255)

                                          cAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgggg                                                  <  1:2364441/93‑1 (MQ=255)

                                          cAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgggg                                                  <  2:6554405/93‑1 (MQ=255)

                                           aGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCg                                                <  1:3648309/93‑1 (MQ=255)

                                            gTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCg                                                >  2:3032008/1‑93 (MQ=255)

                                            gTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGt                                               >  1:4434369/1‑93 (MQ=255)

                                              ggATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGtt                                              <  2:4914023/93‑1 (MQ=255)

                                                aTTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTAt                                            >  1:478155/1‑93 (MQ=255)

                                                 ttAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATg                                           <  1:6099057/93‑1 (MQ=255)

                                                 ttAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATg                                           >  2:3930788/1‑93 (MQ=255)

                                                 ttAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATg                                           >  2:328958/1‑93 (MQ=255)

                                                  tAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgg                                          <  2:2502886/93‑1 (MQ=255)

                                                  tAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgg                                          <  1:3442690/93‑1 (MQ=255)

                                                  tAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgg                                          <  2:5576221/93‑1 (MQ=255)

                                                   aaGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg                                         <  1:6188434/93‑1 (MQ=255)

                                                   aaGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg                                         <  2:2841408/93‑1 (MQ=255)

                                                    agcagcATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgggg                                        <  2:5576362/93‑1 (MQ=255)

                                                     gcagcaTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggggg                                       <  1:4301232/93‑1 (MQ=255)

                                                      cagcaTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGa                                      <  1:3607143/93‑1 (MQ=255)

                                                      caccaTCCCAATTTAAAGGTGCTGAAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGa                                      <  1:5608814/90‑1 (MQ=255)

                                                        gcaTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACa                                    >  2:4612583/1‑93 (MQ=255)

                                                         caTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACAt                                   <  2:5171845/93‑1 (MQ=255)

                                                          aTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACAtt                                  >  1:1637778/1‑93 (MQ=255)

                                                          aTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACAtt                                  <  1:702795/93‑1 (MQ=255)

                                                          aTCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACAtt                                  <  2:1559669/93‑1 (MQ=255)

                                                           tCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCt                                >  2:1719161/1‑93 (MQ=255)

                                                            cccAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGGCATTCt                                >  1:3492638/1‑93 (MQ=255)

                                                               aaTTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTaa                             <  1:4523140/93‑1 (MQ=255)

                                                               aaTTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTaa                             <  1:2727960/93‑1 (MQ=255)

                                                                  ttAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAatat                          >  2:3139972/1‑93 (MQ=255)

                                                                  ttAAAGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGGTATGGGGGACATTCTTAatat                          >  2:3286738/1‑93 (MQ=255)

                                                                    aaaGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAAtattat                       <  2:2176957/93‑1 (MQ=255)

                                                                     aaGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAAtattat                       >  1:4735728/1‑93 (MQ=255)

                                                                      aGGTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATattatt                      >  2:777988/1‑93 (MQ=255)

                                                                       ggTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATAttatta                     >  1:4365525/1‑93 (MQ=255)

                                                                       ggTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATAttatta                     >  2:6155221/1‑93 (MQ=255)

                                                                        gTGCTGGAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTAc                    <  2:5726415/93‑1 (MQ=255)

                                                                             ggAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGc               >  1:3180606/1‑93 (MQ=255)

                                                                              gAAAATCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCa              >  1:4872687/1‑93 (MQ=255)

                                                                                 aaTCCTTACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGCTATGGGGGACATTCTTAATATTATTTCCTTTCAAAc           >  1:4008535/1‑93 (MQ=255)

                                                                                 aaTCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACa          <  1:3668243/93‑1 (MQ=255)

                                                                                    ccTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGt       >  2:3874806/1‑93 (MQ=255)

                                                                                      ttACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGtt      >  2:3119769/1‑93 (MQ=255)

                                                                                      ttACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGtt      >  1:4851018/1‑93 (MQ=255)

                                                                                       tACTCGCCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGttt     >  1:492631/1‑93 (MQ=255)

                                                                                         cTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTaaa  <  1:1490960/93‑1 (MQ=255)

GCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAA  >  NC_000913/2483237‑2483418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCG  >  NC_000913/2483243‑2483428

CTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCC                                                                                              <  SRR4302547.6765375/94‑1 (MQ=60)

 TGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCct                                                                                             >  SRR4302547.3735934/1‑92 (MQ=60)

    ACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATT                                                                                           >  SRR4302547.1290755/1‑94 (MQ=60)

    ACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATT                                                                                           <  SRR4302547.6416586/94‑1 (MQ=60)

    ACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTC                                                                                          <  SRR4302547.5020226/94‑1 (MQ=60)

      AAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCT                                                                                         >  SRR4302547.4244440/1‑94 (MQ=60)

        ACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTAT                                                                                       <  SRR4302547.3306972/94‑1 (MQ=60)

        ACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTAT                                                                                       <  SRR4302547.3557124/94‑1 (MQ=60)

        ACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTAT                                                                                       <  SRR4302547.850874/94‑1 (MQ=60)

         CCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATG                                                                                      <  SRR4302547.1003360/94‑1 (MQ=60)

           ATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGAC                                                                                    >  SRR4302547.5327371/1‑94 (MQ=60)

                AACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CCACCCCTATTCTATGACGGATG                                                                               >  SRR4302547.4456121/1‑94 (MQ=60)

                AACACACTGGACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATG                                                                               >  SRR4302547.6676709/1‑94 (MQ=60)

                  CTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAA                                                                             >  SRR4302547.4594496/1‑94 (MQ=60)

                   TCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAA                                                                            <  SRR4302547.1032105/94‑1 (MQ=60)

                   CCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAA                                                                            <  SRR4302547.120600/94‑1 (MQ=60)

                    CACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAA                                                                           >  SRR4302547.5802831/1‑94 (MQ=60)

                     ACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAAT                                                                          >  SRR4302547.6038456/1‑94 (MQ=60)

                      CTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATG                                                                         >  SRR4302547.5466941/1‑94 (MQ=60)

                      CTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATG                                                                         >  SRR4302547.2819832/1‑94 (MQ=60)

                       TGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGG                                                                        <  SRR4302547.3403406/94‑1 (MQ=60)

                       TGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGG                                                                        >  SRR4302547.4411844/1‑94 (MQ=60)

                        GAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGC                                                                       >  SRR4302547.6374483/1‑94 (MQ=60)

                         AACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCT                                                                      >  SRR4302547.5808472/1‑94 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTC                                                                     <  SRR4302547.2953329/94‑1 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTC                                                                     <  SRR4302547.2025095/94‑1 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTC                                                                     <  SRR4302547.1732287/94‑1 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTC                                                                     >  SRR4302547.917614/1‑94 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTC                                                                     <  SRR4302547.1730660/94‑1 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTC                                                                     <  SRR4302547.612930/94‑1 (MQ=60)

                          ACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCG                                                                    <  SRR4302547.6302801/94‑1 (MQ=60)

                           CATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCG                                                                    <  SRR4302547.252862/94‑1 (MQ=60)

                           CATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCG                                                                    <  SRR4302547.648079/94‑1 (MQ=60)

                             TGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGT                                                                  <  SRR4302547.1627376/94‑1 (MQ=60)

                             TGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTT                                                                 <  SRR4302547.1833520/94‑1 (MQ=60)

                              GAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTT                                                                 <  SRR4302547.3034135/94‑1 (MQ=60)

                                    CAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGC                                                           <  SRR4302547.2412372/94‑1 (MQ=60)

                                     AGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCG                                                          >  SRR4302547.2432709/1‑94 (MQ=60)

                                     AGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGT                                                         >  SRR4302547.4526037/1‑94 (MQ=60)

                                     AGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGT                                                         <  SRR4302547.3722725/94‑1 (MQ=60)

                                         GATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTAT                                                      >  SRR4302547.486940/1‑94 (MQ=60)

                                           TTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGG                                                    <  SRR4302547.6225573/94‑1 (MQ=60)

                                            TAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGG                                                   <  SRR4302547.3512881/94‑1 (MQ=60)

                                            TAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGG                                                   <  SRR4302547.4652339/94‑1 (MQ=60)

                                             AAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGG                                                  <  SRR4302547.6316887/94‑1 (MQ=60)

                                              AGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGG                                                 >  SRR4302547.2963624/1‑94 (MQ=60)

                                               GCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGA                                                <  SRR4302547.4389695/94‑1 (MQ=60)

                                                CAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGAC                                               <  SRR4302547.3680702/94‑1 (MQ=60)

                                                CAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGAC                                               <  SRR4302547.6464848/94‑1 (MQ=60)

                                                CAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGGTATGGGGGGC                                               >  SRR4302547.411705/1‑94 (MQ=60)

                                                CACCATCCCAATTTAAAGGTGCTGAAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGAC                                               <  SRR4302547.5724553/94‑1 (MQ=60)

                                                   CATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATT                                            >  SRR4302547.1670154/1‑94 (MQ=60)

                                                   CATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATT                                            <  SRR4302547.1946945/94‑1 (MQ=60)

                                                    ATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTC                                           <  SRR4302547.715784/94‑1 (MQ=60)

                                                    ATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCT                                          >  SRR4302547.4929018/1‑94 (MQ=60)

                                                     TCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGGCATTCT                                          >  SRR4302547.3563912/1‑94 (MQ=60)

                                                     TCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCT                                          <  SRR4302547.879196/94‑1 (MQ=60)

                                                         AATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAAT                                      <  SRR4302547.4616617/94‑1 (MQ=60)

                                                         AATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAAT                                      >  SRR4302547.3648714/1‑94 (MQ=60)

                                                         AATTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAAT                                      <  SRR4302547.2782727/94‑1 (MQ=60)

                                                           TTTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATAT                                    >  SRR4302547.5972927/1‑94 (MQ=60)

                                                            TTAAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATT                                   <  SRR4302547.2347994/94‑1 (MQ=60)

                                                              AAAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTAT                                 >  SRR4302547.4833881/1‑94 (MQ=60)

                                                               TAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTTTGCGGGGCATTCTTTATATTATT                                >  SRR4302547.3710404/1‑94 (MQ=60)

                                                               AAGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATT                                >  SRR4302547.5715916/1‑94 (MQ=60)

                                                                AGGTGCTGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTA                               >  SRR4302547.4455177/1‑94 (MQ=60)

                                                                  GTGCTGGAAAATCCTTGCTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACC                             <  SRR4302547.3759959/94‑1 (MQ=60)

                                                                      TGGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGC                         >  SRR4302547.3243995/1‑94 (MQ=60)

                                                                       GGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCA                        >  SRR4302547.4973661/1‑94 (MQ=60)

                                                                       GGAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCA                        <  SRR4302547.494765/94‑1 (MQ=60)

                                                                       GGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAA                       >  SRR4302547.832888/1‑94 (MQ=60)

                                                                        GAAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAA                       >  SRR4302547.4272720/1‑94 (MQ=60)

                                                                         AAAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGGTATGGGGGACATTCTTAATATTATTACCTTGCAAA                      >  SRR4302547.6177379/1‑94 (MQ=60)

                                                                          AAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAAC                     >  SRR4302547.3110732/1‑94 (MQ=60)

                                                                          AAATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGCTATGGGGGACATTCTTAATATTATTTCCTTTCAAAC                     >  SRR4302547.4090006/1‑94 (MQ=60)

                                                                           AATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACA                    >  SRR4302547.6064946/1‑94 (MQ=60)

                                                                           AATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACA                    <  SRR4302547.6275271/94‑1 (MQ=60)

                                                                           AATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAG                   <  SRR4302547.3743055/94‑1 (MQ=60)

                                                                           AATCCTTACGCG‑CC‑CCCCTACTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAG                   >  SRR4302547.6587385/1‑94 (MQ=60)

                                                                            ATCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAG                   <  SRR4302547.6391599/94‑1 (MQ=60)

                                                                             TCCTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGG                  >  SRR4302547.1722094/1‑94 (MQ=60)

                                                                              CCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTT                >  SRR4302547.1452346/1‑94 (MQ=60)

                                                                               CTTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTT                >  SRR4302547.4951539/1‑94 (MQ=60)

                                                                                TTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTT               >  SRR4302547.501625/1‑94 (MQ=60)

                                                                                TTACTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGATCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCGAACAGGCTT               >  SRR4302547.6371977/1‑94 (MQ=60)

                                                                                   CTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAA            >  SRR4302547.3152680/1‑94 (MQ=60)

                                                                                   CTCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAA            <  SRR4302547.6369137/94‑1 (MQ=60)

                                                                                   CTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAAT           <  SRR4302547.1520699/94‑1 (MQ=60)

                                                                                    TCGCCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAAT           <  SRR4302547.5078358/94‑1 (MQ=60)

                                                                                     cgcCC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATT          >  SRR4302547.1900658/4‑94 (MQ=60)

                                                                                      G‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTT        >  SRR4302547.2389920/1‑94 (MQ=60)

                                                                                        CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTT       >  SRR4302547.3202908/1‑94 (MQ=60)

                                                                                         C‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTC      >  SRR4302547.3087691/1‑94 (MQ=60)

                                                                                         C‑CCCCTATTCTATGACGGATGAAAATGGCTCGGGTCGGGGCGTTATGGGGGACCTTCTTTATATTATTTCCTTGCAAACAGGTTTAAATTTTTC      >  SRR4302547.440965/1‑94 (MQ=60)

                                                                                           CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCT     <  SRR4302547.5037490/94‑1 (MQ=60)

                                                                                            CCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTC    <  SRR4302547.3431874/94‑1 (MQ=60)

                                                                                            CCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTC    <  SRR4302547.3224530/94‑1 (MQ=60)

                                                                                            CCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTC    <  SRR4302547.4576803/94‑1 (MQ=60)

                                                                                             CCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCC   >  SRR4302547.6007770/1‑94 (MQ=60)

                                                                                             CCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCC   <  SRR4302547.180129/94‑1 (MQ=60)

                                                                                              CTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCG  >  SRR4302547.712805/1‑94 (MQ=60)

                                                                                              CTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCG  >  SRR4302547.4831081/1‑94 (MQ=60)

                                                                                              CTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCG  >  SRR4302547.980371/1‑94 (MQ=60)

CTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCG‑CC‑CCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCG  >  NC_000913/2483243‑2483428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`