Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,488,636 T→C E445E (GAA→GAG)  b2373 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,488,6360TC87.9% 529.9 / 56.9 191E445E (GAA→GAG) b2373hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/8);  new base C (125/42);  total (141/50)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

Only 100 of 196 total aligned reads displayed.

CGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCA  >  NC_000913/2488545‑2488727
                                                                                           |                                                                                           
 gccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTc                                                                                           >  1:5609964/1‑93 (MQ=255)
  ccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCa                                                                                          >  1:5021845/1‑93 (MQ=255)
  ccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCa                                                                                          >  2:3618028/1‑93 (MQ=255)
    attattAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAAt                                                                                        >  1:4086190/1‑93 (MQ=255)
    attattAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAAt                                                                                        >  2:108575/1‑93 (MQ=255)
     ttattaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATg                                                                                       >  1:6338491/1‑93 (MQ=255)
      tattaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATgg                                                                                      >  1:4971273/1‑93 (MQ=255)
      tattaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATgg                                                                                      >  1:577442/1‑93 (MQ=255)
       attaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGc                                                                                     >  1:232621/1‑93 (MQ=255)
       attaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGc                                                                                     <  1:2815561/93‑1 (MQ=255)
       attaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGGTTCAATTTCAATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGc                                                                                     >  2:3357123/1‑93 (MQ=255)
        ttaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCg                                                                                    >  1:584493/1‑93 (MQ=255)
            aaaTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACaa                                                                                >  1:5140649/1‑93 (MQ=255)
            aaaTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACaa                                                                                <  2:2856714/93‑1 (MQ=255)
             aaTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAAc                                                                               <  1:2453561/93‑1 (MQ=255)
             aaTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAAc                                                                               <  2:1735985/93‑1 (MQ=255)
              aTAACGATCGTCGCCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCGCCCTCAATGGCGACAAcc                                                                              <  2:1318014/93‑1 (MQ=255)
                  cGATCGTCAGCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGgag                                                                          <  2:2689657/93‑1 (MQ=255)
                  cGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGgag                                                                          >  1:1915305/1‑93 (MQ=255)
                     tCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGTACTATCACCCTCAATGGAGACAACCGGAGAAc                                                                       >  2:4554336/1‑93 (MQ=255)
                     tCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAAc                                                                       >  2:3958464/1‑93 (MQ=255)
                     tCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAc                                                                       >  1:3053/1‑93 (MQ=255)
                     tCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAc                                                                       >  1:1008030/1‑93 (MQ=255)
                      cGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAcc                                                                      >  2:5149360/1‑93 (MQ=255)
                      cGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAcc                                                                      >  2:3451164/1‑93 (MQ=255)
                      cGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAcc                                                                      >  2:5324555/1‑93 (MQ=255)
                      cGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATCGCGACAACCGGAGAAcc                                                                      >  2:1600944/1‑93 (MQ=255)
                       gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCa                                                                     >  2:4208845/1‑93 (MQ=255)
                       gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCa                                                                     <  1:3145153/93‑1 (MQ=255)
                       gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCa                                                                     <  2:6011896/93‑1 (MQ=255)
                       gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCa                                                                     >  1:1373597/1‑93 (MQ=255)
                        tCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAg                                                                    >  2:2806268/1‑93 (MQ=255)
                        tCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAg                                                                    >  2:375426/1‑93 (MQ=255)
                        tCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAg                                                                    >  1:5072369/1‑93 (MQ=255)
                          aCCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGaa                                                                  >  2:1145658/1‑93 (MQ=255)
                          aCCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGaa                                                                  <  1:1760803/93‑1 (MQ=255)
                           ccGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAg                                                                 >  1:4323588/1‑93 (MQ=255)
                             ggCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTc                                                               <  1:3731091/93‑1 (MQ=255)
                              gCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCa                                                              >  2:1212139/1‑93 (MQ=255)
                                 ggTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                           >  2:233720/1‑93 (MQ=255)
                                 ggTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                           >  1:486901/1‑93 (MQ=255)
                                 ggTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                           <  1:2281273/93‑1 (MQ=255)
                                 ggTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                           >  2:4970008/1‑93 (MQ=255)
                                 ggTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                           >  1:3608028/1‑93 (MQ=255)
                                  gTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCt                                                          <  1:2136541/93‑1 (MQ=255)
                                    tataTCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAg                                                        >  2:3507685/1‑93 (MQ=255)
                                     atatCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGc                                                       >  2:4381028/1‑93 (MQ=255)
                                     atatCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGc                                                       >  1:4760448/1‑93 (MQ=255)
                                      tatCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCa                                                      >  1:4216466/1‑93 (MQ=255)
                                      tatCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCa                                                      >  2:5574164/1‑93 (MQ=255)
                                       atCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCAc                                                     >  2:2495113/1‑93 (MQ=255)
                                       atCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCAc                                                     <  2:5601173/93‑1 (MQ=255)
                                          gACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCCCCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGa                                                  <  1:5351866/93‑1 (MQ=255)
                                          gACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAAACAGAGAACCAGAAGTCACGCTAGCACCGa                                                  >  1:4337104/1‑93 (MQ=255)
                                          gACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGa                                                  <  2:3533539/93‑1 (MQ=255)
                                           aCAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGAt                                                 <  2:4611543/93‑1 (MQ=255)
                                           aCAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGAt                                                 <  2:844549/93‑1 (MQ=255)
                                            cAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATg                                                <  2:3232498/93‑1 (MQ=255)
                                              aaTCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCGCCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGc                                              >  2:3553532/1‑93 (MQ=255)
                                              aaTCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGc                                              >  1:2549361/1‑93 (MQ=255)
                                              aaTCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGc                                              >  1:2962658/1‑93 (MQ=255)
                                               aTCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCa                                             >  2:5953849/1‑93 (MQ=255)
                                                 cGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATa                                           <  1:2576709/93‑1 (MQ=255)
                                                    ttCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGcc                                        >  1:1606676/1‑93 (MQ=255)
                                                       aaTTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCAt                                     >  2:3028471/1‑93 (MQ=255)
                                                        aTTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATa                                    >  1:1086126/1‑93 (MQ=255)
                                                           tCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                 >  2:3121010/1‑93 (MQ=255)
                                                            ccATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGa                                >  1:6410523/1‑93 (MQ=255)
                                                             cATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGAt                               >  2:2594560/1‑93 (MQ=255)
                                                             cATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGAt                               >  2:3172738/1‑93 (MQ=255)
                                                               tCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGc                             >  1:4596220/1‑93 (MQ=255)
                                                               tCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGc                             >  2:2211521/1‑93 (MQ=255)
                                                               tCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGc                             >  2:4697059/1‑93 (MQ=255)
                                                                cccACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGcc                            >  2:1113670/1‑93 (MQ=255)
                                                                cccACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGcc                            >  2:507635/1‑93 (MQ=255)
                                                                 ccACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGccc                           >  2:6618129/1‑93 (MQ=255)
                                                                  cACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCa                          >  1:3220700/1‑93 (MQ=255)
                                                                  cACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCa                          >  1:6307610/1‑93 (MQ=255)
                                                                   aCTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAt                         >  1:3491525/1‑93 (MQ=255)
                                                                   aCTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAt                         >  1:3006893/1‑93 (MQ=255)
                                                                     tGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATACCCCATACCGATGCCCATGa                       >  2:4331387/1‑93 (MQ=255)
                                                                      gAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGac                      >  1:6596345/1‑93 (MQ=255)
                                                                         aCCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                   <  1:5461237/93‑1 (MQ=255)
                                                                          ccAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAccc                  >  2:3783039/1‑93 (MQ=255)
                                                                          ccAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAccc                  <  1:2627343/93‑1 (MQ=255)
                                                                          ccAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAGGACAccc                  <  1:4240304/93‑1 (MQ=255)
                                                                            aaaaGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCa                <  1:5986813/93‑1 (MQ=255)
                                                                               aGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGt             >  2:5294662/1‑93 (MQ=255)
                                                                                gCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTa            >  2:5235737/1‑93 (MQ=255)
                                                                                gCACTATCACCATCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTa            >  1:1667871/1‑93 (MQ=255)
                                                                                 cACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTAc           >  1:6140382/1‑93 (MQ=255)
                                                                                  aCTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTAcc          >  2:276757/1‑93 (MQ=255)
                                                                                   cTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACca         >  2:57114/1‑93 (MQ=255)
                                                                                   cTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACca         >  2:4812122/1‑93 (MQ=255)
                                                                                   cTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACca         >  1:3748856/1‑93 (MQ=255)
                                                                                    tATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACcac        >  2:6283065/1‑93 (MQ=255)
                                                                                      tCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACaa      >  2:2016512/1‑93 (MQ=255)
                                                                                       cACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAAt     <  2:4086190/93‑1 (MQ=255)
                                                                                       cACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAAt     >  2:1487224/1‑93 (MQ=255)
                                                                                          cTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCa  <  1:2174169/93‑1 (MQ=255)
                                                                                           |                                                                                           
CGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCA  >  NC_000913/2488545‑2488727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAG  >  NC_000913/2488543‑2488728
                                                                                             |                                                                                            
GCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCC                                                                                              <  SRR4302547.4906392/94‑1 (MQ=60)
 CCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCT                                                                                             >  SRR4302547.5147470/1‑94 (MQ=60)
 CCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCT                                                                                             >  SRR4302547.3841872/1‑94 (MQ=60)
  CGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTC                                                                                            >  SRR4302547.2200220/1‑94 (MQ=60)
  CGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTC                                                                                            >  SRR4302547.5725722/1‑94 (MQ=60)
   GCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCA                                                                                           >  SRR4302547.5125611/1‑94 (MQ=60)
     CATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAAT                                                                                         <  SRR4302547.2969476/94‑1 (MQ=60)
     CATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAAT                                                                                         >  SRR4302547.4169243/1‑94 (MQ=60)
     CATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAAT                                                                                         >  SRR4302547.4775486/1‑94 (MQ=60)
      ATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATG                                                                                        >  SRR4302547.6470471/1‑94 (MQ=60)
       TTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGG                                                                                       >  SRR4302547.5074123/1‑94 (MQ=60)
       TTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGG                                                                                       >  SRR4302547.587793/1‑94 (MQ=60)
        TATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGC                                                                                      <  SRR4302547.3817199/94‑1 (MQ=60)
        TATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGC                                                                                      >  SRR4302547.236996/1‑94 (MQ=60)
         ATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCG                                                                                     <  SRR4302547.2871876/94‑1 (MQ=60)
         ATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCG                                                                                     >  SRR4302547.595009/1‑94 (MQ=60)
          TTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGA                                                                                    <  SRR4302547.588266/94‑1 (MQ=60)
           TAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGAC                                                                                   >  SRR4302547.1672126/1‑94 (MQ=60)
            AAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACA                                                                                  >  SRR4302547.2608102/1‑94 (MQ=60)
            AAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACA                                                                                  >  SRR4302547.4372131/1‑94 (MQ=60)
             AAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAA                                                                                 >  SRR4302547.5246546/1‑94 (MQ=60)
              AAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAAC                                                                                >  SRR4302547.606302/1‑94 (MQ=60)
               AATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACC                                                                               <  SRR4302547.2503146/94‑1 (MQ=60)
               AATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACC                                                                               >  SRR4302547.1705111/1‑94 (MQ=60)
                ATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCG                                                                              >  SRR4302547.5362743/1‑94 (MQ=60)
                  AACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGA                                                                            >  SRR4302547.4519849/1‑94 (MQ=60)
                   ACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAG                                                                           >  SRR4302547.1953197/1‑94 (MQ=60)
                      ATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAC                                                                        >  SRR4302547.1027711/1‑94 (MQ=60)
                      ATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAAC                                                                        >  SRR4302547.3101/1‑94 (MQ=60)
                        CGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCA                                                                      >  SRR4302547.1401141/1‑94 (MQ=60)
                         GTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAG                                                                     >  SRR4302547.1480284/1‑94 (MQ=60)
                         GTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAG                                                                     >  SRR4302547.5177018/1‑94 (MQ=60)
                         GTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAG                                                                     <  SRR4302547.3207649/94‑1 (MQ=60)
                          TCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCCAAAGCACTATCACCCTCAATGGCGACAACCGGAGAATCAGA                                                                    >  SRR4302547.3043841/1‑94 (MQ=60)
                           CACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAA                                                                   >  SRR4302547.4982583/1‑94 (MQ=60)
                           CACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAA                                                                   >  SRR4302547.988632/1‑94 (MQ=60)
                           CACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAA                                                                   <  SRR4302547.5720124/94‑1 (MQ=60)
                            ACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAG                                                                  >  SRR4302547.4412591/1‑94 (MQ=60)
                            ACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAG                                                                  <  SRR4302547.1795713/94‑1 (MQ=60)
                               GGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCA                                                               >  SRR4302547.3631492/1‑94 (MQ=60)
                               GGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCA                                                               <  SRR4302547.3807270/94‑1 (MQ=60)
                                GCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCAC                                                              >  SRR4302547.2586057/1‑94 (MQ=60)
                                  AGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGC                                                            >  SRR4302547.495817/1‑94 (MQ=60)
                                  AGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGC                                                            >  SRR4302547.3681610/1‑94 (MQ=60)
                                   GGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCT                                                           <  SRR4302547.2327452/94‑1 (MQ=60)
                                    GTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTA                                                          <  SRR4302547.2179102/94‑1 (MQ=60)
                                     TTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAG                                                         >  SRR4302547.6470081/1‑94 (MQ=60)
                                      TATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGC                                                        >  SRR4302547.4859079/1‑94 (MQ=60)
                                       ATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCA                                                       >  SRR4302547.4861854/1‑94 (MQ=60)
                                       ATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCA                                                       >  SRR4302547.4302538/1‑94 (MQ=60)
                                          TCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCA                                                    <  SRR4302547.5828667/94‑1 (MQ=60)
                                           CGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCCCCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGA                                                   <  SRR4302547.2122277/94‑1 (MQ=60)
                                           CGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAAACAGAGAACCAGAAGTCACGCTAGCACCGA                                                   >  SRR4302547.4426315/1‑94 (MQ=60)
                                            GACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCCCCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGAT                                                  <  SRR4302547.5462227/94‑1 (MQ=60)
                                               AAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGC                                               >  SRR4302547.6672641/1‑94 (MQ=60)
                                               AAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGC                                               >  SRR4302547.3021622/1‑94 (MQ=60)
                                               AAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGC                                               >  SRR4302547.2600842/1‑94 (MQ=60)
                                               AAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGC                                               >  SRR4302547.441513/1‑94 (MQ=60)
                                               AAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGC                                               >  SRR4302547.314071/1‑94 (MQ=60)
                                                AATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCA                                              >  SRR4302547.2674243/1‑94 (MQ=60)
                                                   CGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAG                                           <  SRR4302547.2628712/94‑1 (MQ=60)
                                                   CGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAG                                           <  SRR4302547.2715368/94‑1 (MQ=60)
                                                     TTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCC                                         >  SRR4302547.1638527/1‑94 (MQ=60)
                                                        CAATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCAT                                      >  SRR4302547.5189124/1‑94 (MQ=60)
                                                         AATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATA                                     >  SRR4302547.1107829/1‑94 (MQ=60)
                                                          ATTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATAC                                    >  SRR4302547.3332361/1‑94 (MQ=60)
                                                           TTTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACC                                   <  SRR4302547.6333153/94‑1 (MQ=60)
                                                            TTCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCG                                  >  SRR4302547.12025/1‑94 (MQ=60)
                                                             TCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGA                                 <  SRR4302547.5085061/94‑1 (MQ=60)
                                                             TCCATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGA                                 >  SRR4302547.6544214/1‑94 (MQ=60)
                                                              CCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGAT                                >  SRR4302547.2363773/1‑94 (MQ=60)
                                                               CATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATG                               >  SRR4302547.3980727/1‑94 (MQ=60)
                                                                ATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGC                              >  SRR4302547.4691326/1‑94 (MQ=60)
                                                                ATCCCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGC                              >  SRR4302547.550348/1‑94 (MQ=60)
                                                                   CCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCA                           >  SRR4302547.6438751/1‑94 (MQ=60)
                                                                   CCACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCA                           >  SRR4302547.3284940/1‑94 (MQ=60)
                                                                    CACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAT                          >  SRR4302547.3562776/1‑94 (MQ=60)
                                                                    CACTGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAT                          >  SRR4302547.3066693/1‑94 (MQ=60)
                                                                       TGAAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGAC                       >  SRR4302547.6733674/1‑94 (MQ=60)
                                                                         AAACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAC                     >  SRR4302547.2478632/1‑94 (MQ=60)
                                                                          AACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACC                    >  SRR4302547.3282013/1‑94 (MQ=60)
                                                                           ACCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCC                   <  SRR4302547.5574057/94‑1 (MQ=60)
                                                                            CCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCC                  <  SRR4302547.2680265/94‑1 (MQ=60)
                                                                            CCAAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAGGACACCCC                  <  SRR4302547.4327015/94‑1 (MQ=60)
                                                                              AAAAGCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAG                <  SRR4302547.6111011/94‑1 (MQ=60)
                                                                                 AGCACTATCACCATCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTA             >  SRR4302547.1700747/1‑94 (MQ=60)
                                                                                  GCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTAC            >  SRR4302547.6267758/1‑94 (MQ=60)
                                                                                  GCACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTAC            >  SRR4302547.6486601/1‑94 (MQ=60)
                                                                                   CACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACC           >  SRR4302547.5131395/1‑94 (MQ=60)
                                                                                    ACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCA          >  SRR4302547.3825379/1‑94 (MQ=60)
                                                                                    ACTATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCA          >  SRR4302547.1812688/1‑94 (MQ=60)
                                                                                      TATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACA        >  SRR4302547.4323716/1‑94 (MQ=60)
                                                                                      TATCACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACA        >  SRR4302547.3069601/1‑94 (MQ=60)
                                                                                         CACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATC     >  SRR4302547.2250752/1‑94 (MQ=60)
                                                                                         CACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATC     <  SRR4302547.6209590/94‑1 (MQ=60)
                                                                                          ACCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCC    >  SRR4302547.3826966/1‑94 (MQ=60)
                                                                                           CCCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCA   <  SRR4302547.784000/94‑1 (MQ=60)
                                                                                            CTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAG  <  SRR4302547.2217693/94‑1 (MQ=60)
                                                                                            CTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAG  <  SRR4302547.3684858/94‑1 (MQ=60)
                                                                                            CCTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAG  <  SRR4302547.759119/94‑1 (MQ=60)
                                                                                             |                                                                                            
GCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAG  >  NC_000913/2488543‑2488728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑