Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,495,116 T→C L400L (CTA→CTG)  b2379 ← putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,495,1160TC89.4% 650.5 / 49.1 226L400L (CTA→CTG) b2379putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (14/10);  new base C (100/102);  total (114/112)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

Only 100 of 238 total aligned reads displayed.

AATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGC  >  NC_000913/2495026‑2495206
                                                                                          |                                                                                          
     aaaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAAc                                                                                     <  1:6642548/93‑1 (MQ=255)
     aaaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAAc                                                                                     <  1:6632500/93‑1 (MQ=255)
     aaaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCCCGTTTTCGTGAGTATGTTTGCTGCTGCCGCGTATCAAAc                                                                                     >  1:6251394/1‑93 (MQ=255)
      aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAAcc                                                                                    >  1:5241522/1‑93 (MQ=255)
      aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAAcc                                                                                    <  1:335824/93‑1 (MQ=255)
      aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAAcc                                                                                    >  1:5691224/1‑93 (MQ=255)
      aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAAcc                                                                                    <  2:592329/93‑1 (MQ=255)
        aCAGTAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGt                                                                                  <  1:2680622/93‑1 (MQ=255)
         cAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTc                                                                                 >  1:235484/1‑93 (MQ=255)
          aGGGGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGTAAACCGTCg                                                                                >  1:198606/1‑93 (MQ=255)
           ggAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCgg                                                                               >  1:6460058/1‑93 (MQ=255)
            gAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGGAAACCGTCggg                                                                              >  1:4774864/1‑92 (MQ=255)
            gAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGc                                                                              >  2:4454992/1‑93 (MQ=255)
              gCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCAc                                                                            >  1:4196964/1‑93 (MQ=255)
              gCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCAc                                                                            <  2:6290439/93‑1 (MQ=255)
                 aTCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGa                                                                         >  1:3723425/1‑93 (MQ=255)
                 aTCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGa                                                                         >  1:3589137/1‑93 (MQ=255)
                   cAGGCTCGTGTTTGCATTTCTGCAGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGACTAAACCGTCGGGACGGagg                                                                       >  1:1902829/1‑91 (MQ=255)
                    aGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACa                                                                      <  1:2067483/93‑1 (MQ=255)
                     ggCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAt                                                                     >  1:5404452/1‑93 (MQ=255)
                     ggCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAt                                                                     >  1:3922647/1‑93 (MQ=255)
                     ggCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAt                                                                     <  1:5851825/93‑1 (MQ=255)
                      gCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATc                                                                    >  2:5457911/1‑93 (MQ=255)
                      gCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATc                                                                    <  1:5911360/93‑1 (MQ=255)
                       cTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCg                                                                   >  1:2820321/1‑93 (MQ=255)
                          cTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCtt                                                                <  2:4089771/93‑1 (MQ=255)
                          cTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCtt                                                                <  1:2110341/93‑1 (MQ=255)
                            gTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTa                                                              >  1:2147575/1‑93 (MQ=255)
                             tttGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTaa                                                             <  2:3535263/93‑1 (MQ=255)
                              ttGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAAt                                                            >  2:1548616/1‑93 (MQ=255)
                               tGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATc                                                           <  2:5796525/93‑1 (MQ=255)
                                   tttCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCctc                                                       <  1:4671969/93‑1 (MQ=255)
                                     tCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTg                                                     <  1:15172/93‑1 (MQ=255)
                                     tCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTg                                                     <  1:5142485/93‑1 (MQ=255)
                                      cTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGa                                                    >  2:4487818/1‑93 (MQ=255)
                                       tGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGAt                                                   <  1:9358/93‑1 (MQ=255)
                                         acgacgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATgg                                                 >  1:2723288/1‑93 (MQ=255)
                                          cgacgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGc                                                >  1:3690894/1‑93 (MQ=255)
                                          cgacgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGc                                                <  2:6156458/93‑1 (MQ=255)
                                           gacgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc                                               <  2:4732184/93‑1 (MQ=255)
                                           gacgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc                                               <  2:5308823/93‑1 (MQ=255)
                                           gacgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc                                               >  2:3862363/1‑93 (MQ=255)
                                            acgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt                                              >  1:1066166/1‑93 (MQ=255)
                                            acgGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt                                              >  1:1667870/1‑93 (MQ=255)
                                             cgGGGTTTATTCCGCGTTTACGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCCTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTg                                             >  2:4976048/1‑93 (MQ=255)
                                              ggTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGa                                            <  1:639884/93‑1 (MQ=255)
                                              ggTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGa                                            >  2:5684994/1‑93 (MQ=255)
                                                 gtTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGa                                         >  1:829896/1‑93 (MQ=255)
                                                  tttATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGaa                                        <  2:6462359/93‑1 (MQ=255)
                                                   ttATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAAt                                       <  2:4229918/93‑1 (MQ=255)
                                                     aTTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATAc                                     <  1:4910470/93‑1 (MQ=255)
                                                     aTTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATAc                                     <  1:4254930/93‑1 (MQ=255)
                                                      ttCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACg                                    >  2:320576/1‑93 (MQ=255)
                                                        ccGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGt                                  <  2:298821/93‑1 (MQ=255)
                                                          gcgTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcg                                >  2:265087/1‑93 (MQ=255)
                                                          gcgTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcg                                >  1:3467818/1‑93 (MQ=255)
                                                           cgTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcgc                               <  1:6156387/93‑1 (MQ=255)
                                                            gTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcgcg                              <  1:3246494/93‑1 (MQ=255)
                                                             ttttCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCgg                             <  1:2391572/93‑1 (MQ=255)
                                                             ttttCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCgg                             >  2:2331141/1‑93 (MQ=255)
                                                             ttttCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCgg                             <  2:2511305/93‑1 (MQ=255)
                                                             ttttCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCgg                             <  1:1327181/93‑1 (MQ=255)
                                                              tttCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGt                            >  2:6537593/1‑93 (MQ=255)
                                                              tttCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGt                            <  1:893880/93‑1 (MQ=255)
                                                                tCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCTGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt                          <  2:2105144/93‑1 (MQ=255)
                                                                tCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt                          >  1:6077273/1‑93 (MQ=255)
                                                                tCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt                          >  1:3501251/1‑93 (MQ=255)
                                                                tCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt                          <  1:2473431/93‑1 (MQ=255)
                                                                 tgTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtttt                         <  1:323225/92‑1 (MQ=255)
                                                                 cGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtttt                         >  2:833027/1‑93 (MQ=255)
                                                                 cGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtttt                         <  1:1579285/93‑1 (MQ=255)
                                                                   tGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCa                       >  2:2600260/1‑93 (MQ=255)
                                                                     aaTATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAAt                     <  2:3282881/93‑1 (MQ=255)
                                                                      atatGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATc                    <  1:3288481/93‑1 (MQ=255)
                                                                       tatGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCa                   >  2:5070142/1‑93 (MQ=255)
                                                                        atGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAg                  <  2:3138811/93‑1 (MQ=255)
                                                                         tGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt                 >  2:2842665/1‑93 (MQ=255)
                                                                         tGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACTAATACGGTCGCGGTTTTCAATCAGt                 >  2:4580654/1‑93 (MQ=255)
                                                                         tGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt                 >  1:6506891/1‑93 (MQ=255)
                                                                         tGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt                 <  2:128757/93‑1 (MQ=255)
                                                                           tttGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGc               >  2:2912129/1‑93 (MQ=255)
                                                                            ttGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCa              >  2:1737629/1‑93 (MQ=255)
                                                                             tgctgcTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaa             >  1:138750/1‑93 (MQ=255)
                                                                              gctgctGGCGGGTAGTAACCCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaaa            <  2:2348136/93‑1 (MQ=255)
                                                                              gctgctGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaaa            <  2:113644/93‑1 (MQ=255)
                                                                              gctgctGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaaa            <  2:144420/93‑1 (MQ=255)
                                                                              gctgctGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaaa            >  2:1871542/1‑93 (MQ=255)
                                                                              gctgctGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaaa            <  1:4016095/93‑1 (MQ=255)
                                                                              gctgctGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCaaa            <  1:450892/93‑1 (MQ=255)
                                                                                tgctgGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGc          >  2:387334/1‑93 (MQ=255)
                                                                                 gctgGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCg         >  2:4675791/1‑93 (MQ=255)
                                                                                 gctgGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCg         >  2:431706/1‑93 (MQ=255)
                                                                                   tgGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGaa       >  2:560370/1‑93 (MQ=255)
                                                                                    ggCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAAc      >  2:3662123/1‑93 (MQ=255)
                                                                                     gCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACa     <  1:5007866/93‑1 (MQ=255)
                                                                                     gCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACa     <  1:2920970/93‑1 (MQ=255)
                                                                                       gggTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATg   >  1:430360/1‑93 (MQ=255)
                                                                                       gggCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATg   <  1:4560733/93‑1 (MQ=255)
                                                                                       gggCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATg   <  1:5517101/93‑1 (MQ=255)
                                                                                        ggTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGc  >  2:2162272/1‑93 (MQ=255)
                                                                                          |                                                                                          
AATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGC  >  NC_000913/2495026‑2495206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGCGT  >  NC_000913/2495025‑2495208
                                                                                           |                                                                                            
CAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAG                                                                                            <  SRR4302547.658981/94‑1 (MQ=60)
   TGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAA                                                                                         <  SRR4302547.3314702/94‑1 (MQ=60)
    GAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAA                                                                                        >  SRR4302547.6407136/1‑94 (MQ=60)
     AAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAAC                                                                                       >  SRR4302547.1628697/1‑94 (MQ=60)
      AAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACC                                                                                      >  SRR4302547.5349415/1‑94 (MQ=60)
      AAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACC                                                                                      >  SRR4302547.5808759/1‑94 (MQ=60)
      AAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACC                                                                                      <  SRR4302547.5955480/94‑1 (MQ=60)
      AAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACC                                                                                      <  SRR4302547.6770562/94‑1 (MQ=60)
      AAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACC                                                                                      <  SRR4302547.6780835/94‑1 (MQ=60)
       AAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCG                                                                                     <  SRR4302547.342073/94‑1 (MQ=60)
        AACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGT                                                                                    >  SRR4302547.2848828/1‑94 (MQ=60)
         ACAGTAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTC                                                                                   <  SRR4302547.2734449/94‑1 (MQ=60)
         ACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTC                                                                                   >  SRR4302547.239914/1‑94 (MQ=60)
         ACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTC                                                                                   <  SRR4302547.749643/94‑1 (MQ=60)
          CAGGGGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGTAAACCGTCG                                                                                  >  SRR4302547.202314/1‑94 (MQ=60)
          CAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCG                                                                                  <  SRR4302547.329530/94‑1 (MQ=60)
          CAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCG                                                                                  <  SRR4302547.3420222/94‑1 (MQ=60)
           AGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGG                                                                                 >  SRR4302547.6594590/1‑94 (MQ=60)
            GGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGGAAACCGTCGGG                                                                                >  SRR4302547.4873793/1‑94 (MQ=60)
              AGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCAC                                                                              >  SRR4302547.4282555/1‑94 (MQ=60)
              AGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCAC                                                                              <  SRR4302547.4390560/94‑1 (MQ=60)
                CCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGG                                                                            >  SRR4302547.2532984/1‑94 (MQ=60)
                CCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGG                                                                            >  SRR4302547.5486882/1‑94 (MQ=60)
                 CATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGA                                                                           >  SRR4302547.3799434/1‑94 (MQ=60)
                 CATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGA                                                                           >  SRR4302547.3662356/1‑94 (MQ=60)
                 CATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGA                                                                           >  SRR4302547.6544521/1‑94 (MQ=60)
                 CATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGCCGGCACGGA                                                                           >  SRR4302547.5012038/1‑94 (MQ=60)
                 CATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTGTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGA                                                                           <  SRR4302547.4104967/94‑1 (MQ=60)
                   TCAGGCTCGTGTTTGCATTTCTGCAGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGACTAAACCGTCGGGACGGAgg                                                                         >  SRR4302547.1940472/1‑92 (MQ=60)
                   TCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAAC                                                                         <  SRR4302547.1551675/94‑1 (MQ=60)
                    CAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACA                                                                        >  SRR4302547.3676169/1‑94 (MQ=60)
                     AGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACAT                                                                       >  SRR4302547.3860339/1‑94 (MQ=60)
                     AGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAT                                                                       >  SRR4302547.4002409/1‑94 (MQ=60)
                     AGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAT                                                                       <  SRR4302547.2108740/94‑1 (MQ=60)
                     AGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAT                                                                       >  SRR4302547.5484196/1‑94 (MQ=60)
                     AGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAT                                                                       >  SRR4302547.5516165/1‑94 (MQ=60)
                     AGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACAT                                                                       <  SRR4302547.4055061/94‑1 (MQ=60)
                      GGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATC                                                                      <  SRR4302547.5972870/94‑1 (MQ=60)
                       GCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCG                                                                     >  SRR4302547.2043064/1‑94 (MQ=60)
                       GCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCG                                                                     <  SRR4302547.6033796/94‑1 (MQ=60)
                       GCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCG                                                                     >  SRR4302547.2876741/1‑94 (MQ=60)
                        CTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGC                                                                    >  SRR4302547.5872841/1‑94 (MQ=60)
                         TCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCT                                                                   <  SRR4302547.860138/94‑1 (MQ=60)
                         TCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCT                                                                   <  SRR4302547.6254442/94‑1 (MQ=60)
                          CCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTT                                                                  <  SRR4302547.2712198/94‑1 (MQ=60)
                          CCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGTACATCGATT                                                                  >  SRR4302547.4174375/1‑94 (MQ=60)
                           CTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTT                                                                 >  SRR4302547.284724/1‑94 (MQ=60)
                           CTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTT                                                                 <  SRR4302547.3579711/94‑1 (MQ=60)
                           CTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTT                                                                 <  SRR4302547.2152383/94‑1 (MQ=60)
                            TGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTA                                                                <  SRR4302547.6121767/94‑1 (MQ=60)
                            TGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTA                                                                >  SRR4302547.2190429/1‑94 (MQ=60)
                             GTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAA                                                               >  SRR4302547.3480188/1‑94 (MQ=60)
                               TTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATC                                                             >  SRR4302547.3768891/1‑94 (MQ=60)
                               TTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATC                                                             >  SRR4302547.4320455/1‑94 (MQ=60)
                                TGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCC                                                            <  SRR4302547.3153358/94‑1 (MQ=60)
                                  CATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCT                                                          <  SRR4302547.2740289/94‑1 (MQ=60)
                                    TTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCT                                                        <  SRR4302547.4768716/94‑1 (MQ=60)
                                      TCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGA                                                      <  SRR4302547.5248408/94‑1 (MQ=60)
                                      TCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGA                                                      <  SRR4302547.15450/94‑1 (MQ=60)
                                       CTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGAT                                                     >  SRR4302547.4478544/1‑94 (MQ=60)
                                        TGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATG                                                    >  SRR4302547.6197738/1‑94 (MQ=60)
                                        TGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATG                                                    <  SRR4302547.9530/94‑1 (MQ=60)
                                         GACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGG                                                   >  SRR4302547.2777971/1‑94 (MQ=60)
                                         GACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGG                                                   >  SRR4302547.6627279/1‑94 (MQ=60)
                                          ACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGC                                                  >  SRR4302547.3766246/1‑94 (MQ=60)
                                           CGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCC                                                 <  SRR4302547.2442787/94‑1 (MQ=60)
                                           CGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCC                                                 >  SRR4302547.950285/1‑94 (MQ=60)
                                           CGACGGCGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTACACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCC                                                 >  SRR4302547.707200/1‑94 (MQ=60)
                                            GACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCT                                                >  SRR4302547.4828527/1‑94 (MQ=60)
                                            GACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCT                                                >  SRR4302547.4638022/1‑94 (MQ=60)
                                            GACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCT                                                <  SRR4302547.3463484/94‑1 (MQ=60)
                                            GACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCT                                                >  SRR4302547.1087391/1‑94 (MQ=60)
                                            GACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCT                                                >  SRR4302547.1700746/1‑94 (MQ=60)
                                               GGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGAC                                             <  SRR4302547.651686/94‑1 (MQ=60)
                                               GGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGAC                                             <  SRR4302547.596046/94‑1 (MQ=60)
                                               GGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGAC                                             >  SRR4302547.3553676/1‑94 (MQ=60)
                                               GGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTCAATCCCTCTGATGGCCTGAC                                             <  SRR4302547.6632966/94‑1 (MQ=60)
                                                GTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACG                                            <  SRR4302547.1546214/94‑1 (MQ=60)
                                                GTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACG                                            <  SRR4302547.664967/94‑1 (MQ=60)
                                                 TGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGA                                           <  SRR4302547.1881518/94‑1 (MQ=60)
                                                 TGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGA                                           <  SRR4302547.573955/94‑1 (MQ=60)
                                                 TGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGA                                           >  SRR4302547.845744/1‑94 (MQ=60)
                                                    TTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATA                                        >  SRR4302547.3602015/1‑94 (MQ=60)
                                                     TATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATTCCTCTGATTGCCTGACCAATAC                                       >  SRR4302547.3586314/1‑94 (MQ=60)
                                                     TATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATAC                                       <  SRR4302547.2405380/94‑1 (MQ=60)
                                                      ATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACG                                      <  SRR4302547.5012154/94‑1 (MQ=60)
                                                      ATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACG                                      <  SRR4302547.4341988/94‑1 (MQ=60)
                                                      ATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACG                                      <  SRR4302547.1357819/94‑1 (MQ=60)
                                                          CGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCG                                  >  SRR4302547.3538531/1‑94 (MQ=60)
                                                           GCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGCACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGC                                 >  SRR4302547.2877576/1‑94 (MQ=60)
                                                           GCGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGC                                 >  SRR4302547.844954/1‑94 (MQ=60)
                                                            CGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCG                                <  SRR4302547.330325/94‑1 (MQ=60)
                                                            CGTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCG                                <  SRR4302547.6284152/94‑1 (MQ=60)
                                                             GTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGG                               <  SRR4302547.3311347/94‑1 (MQ=60)
                                                             GTTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGG                               <  SRR4302547.5092416/94‑1 (MQ=60)
                                                              TTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGT                              <  SRR4302547.1353855/94‑1 (MQ=60)
                                                              TTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGT                              <  SRR4302547.6339635/94‑1 (MQ=60)
                                                              TTTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGT                              <  SRR4302547.2440024/94‑1 (MQ=60)
                                                               TTTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTT                             <  SRR4302547.911065/94‑1 (MQ=60)
                                                                TTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTT                            >  SRR4302547.6203335/1‑94 (MQ=60)
                                                                TTCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTT                            >  SRR4302547.3572702/1‑94 (MQ=60)
                                                                 TCGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTT                           <  SRR4302547.2523436/94‑1 (MQ=60)
                                                                  TGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTC                          <  SRR4302547.329240/94‑1 (MQ=60)
                                                                  CGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTC                          <  SRR4302547.1610717/94‑1 (MQ=60)
                                                                  CGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTC                          >  SRR4302547.2170004/1‑94 (MQ=60)
                                                                  CGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTC                          >  SRR4302547.3963910/1‑94 (MQ=60)
                                                                  CGTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGGTTTC                          >  SRR4302547.2097860/1‑94 (MQ=60)
                                                                   GTGAATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCA                         >  SRR4302547.2109417/1‑94 (MQ=60)
                                                                       ATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCA                     <  SRR4302547.3354478/94‑1 (MQ=60)
                                                                       ATATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCA                     >  SRR4302547.4519069/1‑94 (MQ=60)
                                                                        TATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAG                    >  SRR4302547.6779054/1‑94 (MQ=60)
                                                                         ATGTTTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGT                   >  SRR4302547.6642354/1‑94 (MQ=60)
                                                                             TTGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAA               >  SRR4302547.141256/1‑94 (MQ=60)
                                                                              TGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAA              <  SRR4302547.2343911/94‑1 (MQ=60)
                                                                              TGCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAA              <  SRR4302547.4736440/94‑1 (MQ=60)
                                                                               GCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAG             <  SRR4302547.459256/94‑1 (MQ=60)
                                                                               GCTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAG             <  SRR4302547.4097708/94‑1 (MQ=60)
                                                                                CTGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGC            >  SRR4302547.5341327/1‑94 (MQ=60)
                                                                                 TGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGATGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCG           >  SRR4302547.5520551/1‑94 (MQ=60)
                                                                                 TGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCG           >  SRR4302547.2331626/1‑94 (MQ=60)
                                                                                 TGCTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCG           <  SRR4302547.5202951/94‑1 (MQ=60)
                                                                                   CTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAA         <  SRR4302547.4830055/94‑1 (MQ=60)
                                                                                   CTGGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAA         >  SRR4302547.1292560/1‑94 (MQ=60)
                                                                                     GGCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACA       <  SRR4302547.5052923/94‑1 (MQ=60)
                                                                                      GCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACAT      <  SRR4302547.2979208/94‑1 (MQ=60)
                                                                                      GCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACAT      <  SRR4302547.5111407/94‑1 (MQ=60)
                                                                                      GCGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACAT      <  SRR4302547.1522346/94‑1 (MQ=60)
                                                                                       CGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATG     >  SRR4302547.438329/1‑94 (MQ=60)
                                                                                       CGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATG     >  SRR4302547.6402813/1‑94 (MQ=60)
                                                                                       CGGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATG     >  SRR4302547.171354/1‑94 (MQ=60)
                                                                                       CGGGCAGTAAACAGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATG     <  SRR4302547.1986963/94‑1 (MQ=60)
                                                                                        GGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGC    <  SRR4302547.5630992/94‑1 (MQ=60)
                                                                                        GGGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGC    <  SRR4302547.4655085/94‑1 (MQ=60)
                                                                                         GGCAGTAAACCGTCGGCACGGAACATCGCTTTAATTCCTCTGATGGCATGACGCATACGGTCGCGGGTTTCAATCAACGCAAAGCGAACATact   >  SRR4302547.5447948/1‑91 (MQ=60)
                                                                                         GGCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGCG   >  SRR4302547.895169/1‑94 (MQ=60)
                                                                                          GCAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGCGT  >  SRR4302547.506472/1‑94 (MQ=60)
                                                                                           |                                                                                            
CAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGCAAAGCGAACATGCGT  >  NC_000913/2495025‑2495208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑