Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,502,384	T→C	N41S (AAC→AGC)	b2383 ←	putative PTS system enzyme IIA component, enzyme I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,502,384	0	T	C	84.7%	643.1 / 101.2	254	N41S (AAC→AGC)	b2383	putative PTS system enzyme IIA component, enzyme I
Reads supporting (aligned to +/- strand): ref base T (20/19); new base C (102/113); total (122/132)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 262 total aligned reads displayed.

TCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTA  >  NC_000913/2502294‑2502473

tCGCTGCCGCTAATGTTCAGGCTGCAACTGTCCTTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGCGCTTTTCGCATCTGCCTTTGCGCTc                                                                                         >  1:6618407/1‑93 (MQ=255)

tCGCTGCCGCTAATGTTCAAGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTc                                                                                         <  2:4185186/93‑1 (MQ=255)

 cGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGctct                                                                                        <  2:2930850/93‑1 (MQ=255)

  gCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTg                                                                                       >  2:3081547/1‑93 (MQ=255)

  gCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTg                                                                                       <  1:749811/93‑1 (MQ=255)

   cTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTgg                                                                                      <  1:990153/93‑1 (MQ=255)

    tGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGc                                                                                     <  1:4552760/93‑1 (MQ=255)

       cGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGAt                                                                                  <  2:5387630/93‑1 (MQ=255)

       cGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGAt                                                                                  >  2:3372909/1‑93 (MQ=255)

        gCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATg                                                                                 >  1:2974407/1‑93 (MQ=255)

        gCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATg                                                                                 >  2:4577702/1‑93 (MQ=255)

         cTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATgg                                                                                >  1:5184600/1‑93 (MQ=255)

          tAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGt                                                                               <  1:54998/93‑1 (MQ=255)

          tAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGt                                                                               >  1:6096537/1‑93 (MQ=255)

           aaTGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGtt                                                                              >  2:6160652/1‑93 (MQ=255)

           aaTGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGtt                                                                              >  2:3177294/1‑93 (MQ=255)

            aTGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTta                                                                             <  1:3466306/93‑1 (MQ=255)

            aTGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCGCTGGCGATGGTta                                                                             >  2:3259911/1‑93 (MQ=255)

             tGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTtaa                                                                            <  1:3136440/93‑1 (MQ=255)

             tGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTtaa                                                                            <  1:2583220/93‑1 (MQ=255)

              gTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTtaat                                                                           >  1:2258/1‑93 (MQ=255)

               ttCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTtaata                                                                          <  1:3942018/93‑1 (MQ=255)

                 cAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATaaa                                                                        >  2:6271486/1‑93 (MQ=255)

                   ggCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAg                                                                      >  1:2664178/1‑93 (MQ=255)

                    gCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGt                                                                     >  1:6595071/1‑93 (MQ=255)

                    gCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGt                                                                     <  1:4895167/93‑1 (MQ=255)

                       gCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGAt                                                                  <  1:1880753/93‑1 (MQ=255)

                         aaCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGTCTTTGCGCTCTGGCGATGGTTAATAAAAGTGActt                                                                >  1:3960588/1‑93 (MQ=255)

                         aaCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGAttt                                                                >  2:6130469/1‑93 (MQ=255)

                          aCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTc                                                               <  2:3076022/93‑1 (MQ=255)

                          aCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTc                                                               <  1:1340573/93‑1 (MQ=255)

                          aCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTc                                                               <  2:3865913/93‑1 (MQ=255)

                          aCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTc                                                               >  2:3910477/1‑93 (MQ=255)

                          aCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTc                                                               <  2:1670748/93‑1 (MQ=255)

                          aCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTc                                                               >  2:4739666/1‑93 (MQ=255)

                           cTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCg                                                              <  1:2527853/93‑1 (MQ=255)

                             gTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCt                                                            >  2:6046999/1‑93 (MQ=255)

                             gTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCt                                                            <  2:2325357/93‑1 (MQ=255)

                             gTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCt                                                            >  1:2457954/1‑93 (MQ=255)

                              tCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCtt                                                           >  2:488984/1‑93 (MQ=255)

                              tCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCtt                                                           <  2:5810342/93‑1 (MQ=255)

                               cATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCttt                                                          <  2:403010/93‑1 (MQ=255)

                               cATTAAATAGGGTGCCGGTGCCAATAAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCttt                                                          <  1:4384594/93‑1 (MQ=255)

                                  tAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGcc                                                       >  2:3975564/1‑93 (MQ=255)

                                   aaaTAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCa                                                      <  2:1785913/93‑1 (MQ=255)

                                    aaTAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCAc                                                     >  2:5778525/1‑93 (MQ=255)

                                      tAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTg                                                   <  2:192477/93‑1 (MQ=255)

                                      tAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTg                                                   >  2:2411106/1‑93 (MQ=255)

                                      tAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTg                                                   <  1:6362496/93‑1 (MQ=255)

                                        gggTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGc                                                 <  1:1758463/93‑1 (MQ=255)

                                          gtgccggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTg                                               >  2:5049946/1‑93 (MQ=255)

                                           tgccggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGc                                              >  2:2489388/1‑93 (MQ=255)

                                               ggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTg                                          >  1:3640598/1‑93 (MQ=255)

                                               ggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTg                                          >  1:4658563/1‑93 (MQ=255)

                                                  gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTc                                       >  2:52265/1‑93 (MQ=255)

                                                  gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTc                                       >  1:811069/1‑93 (MQ=255)

                                                  gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTc                                       >  2:5333430/1‑93 (MQ=255)

                                                  gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTc                                       >  2:132766/1‑93 (MQ=255)

                                                   ccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCt                                      >  2:389177/1‑93 (MQ=255)

                                                     aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCttt                                    <  2:1269771/93‑1 (MQ=255)

                                                     aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCttt                                    <  2:1462138/93‑1 (MQ=255)

                                                     aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCttt                                    >  1:5808334/1‑93 (MQ=255)

                                                     aaTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCttt                                    >  1:1045946/1‑93 (MQ=255)

                                                      aTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTa                                   <  2:1198961/93‑1 (MQ=255)

                                                      aTCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTa                                   <  1:827864/93‑1 (MQ=255)

                                                       tCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTaa                                  >  1:5612489/1‑93 (MQ=255)

                                                       tCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTaa                                  <  2:5131515/93‑1 (MQ=255)

                                                            gcCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGCGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGttgc                             >  1:4708965/1‑91 (MQ=255)

                                                             ccAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTccc                            <  2:2147740/93‑1 (MQ=255)

                                                             ccAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTccc                            <  2:4079245/93‑1 (MQ=255)

                                                              cAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCa                           <  2:2299087/93‑1 (MQ=255)

                                                               aGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCAc                          <  1:2162191/93‑1 (MQ=255)

                                                               aGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCAc                          <  1:1651240/93‑1 (MQ=255)

                                                               aGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCAc                          <  1:4128264/93‑1 (MQ=255)

                                                               aGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGGTCTTTAAGTTccccc                          >  1:2863354/1‑91 (MQ=255)

                                                                gCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACg                         <  1:1971521/93‑1 (MQ=255)

                                                                gCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACg                         >  2:640872/1‑93 (MQ=255)

                                                                gCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACg                         >  2:2665911/1‑93 (MQ=255)

                                                                 cGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGc                        >  1:3834334/1‑93 (MQ=255)

                                                                   aGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCg                      >  2:5653039/1‑93 (MQ=255)

                                                                   aGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCg                      >  1:5212576/1‑93 (MQ=255)

                                                                    gCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCgg                     <  2:21792/93‑1 (MQ=255)

                                                                    gCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCgg                     >  2:154270/1‑93 (MQ=255)

                                                                       tttCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACg                  <  1:3112840/93‑1 (MQ=255)

                                                                        ttCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGa                 <  2:4253109/93‑1 (MQ=255)

                                                                         tCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAg                >  2:202532/1‑93 (MQ=255)

                                                                         tCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAg                >  2:3623565/1‑93 (MQ=255)

                                                                         tCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAg                <  1:5333957/93‑1 (MQ=255)

                                                                          cGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGc               >  2:290906/1‑93 (MQ=255)

                                                                           gCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCa              <  2:5419303/93‑1 (MQ=255)

                                                                             aTCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATg            >  1:5587244/1‑93 (MQ=255)

                                                                              tCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGt           >  1:4299519/1‑93 (MQ=255)

                                                                                tGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAg         >  1:2721483/1‑93 (MQ=255)

                                                                                tGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAg         >  1:2002819/1‑93 (MQ=255)

                                                                                 gCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGa        <  1:3588068/93‑1 (MQ=255)

                                                                                 gCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGa        >  2:2475451/1‑93 (MQ=255)

                                                                                     ttGCGCTCTGGCGATGATTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGt    <  2:3693212/93‑1 (MQ=255)

                                                                                      tGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTCCCCACGCCGGACGAGCATGTAGACCGtt   <  1:4056369/93‑1 (MQ=255)

                                                                                       gcgcTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTa  <  1:6643730/93‑1 (MQ=255)

                                                                                       gcgcTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTa  <  1:611894/93‑1 (MQ=255)

TCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTA  >  NC_000913/2502294‑2502473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGC  >  NC_000913/2502292‑2502476

CATCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCT                                                                                             <  SRR4302547.2373145/94‑1 (MQ=60)

 ATCGCTGCCGCTAATGTTCAGGCTGCAACTGTCCTTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGCGCTTTTCGCATCTGCCTTTGCGCTC                                                                                            >  SRR4302547.6756158/1‑94 (MQ=60)

  TCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCT                                                                                           >  SRR4302547.5629933/1‑94 (MQ=60)

  TCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCT                                                                                           <  SRR4302547.2935272/94‑1 (MQ=60)

   CGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTG                                                                                          >  SRR4302547.2644633/1‑94 (MQ=60)

    GCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGG                                                                                         <  SRR4302547.763948/94‑1 (MQ=60)

     CTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGC                                                                                        <  SRR4302547.1009430/94‑1 (MQ=60)

     CTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGC                                                                                        <  SRR4302547.3248758/94‑1 (MQ=60)

      TGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCG                                                                                       <  SRR4302547.4646936/94‑1 (MQ=60)

      TGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCG                                                                                       <  SRR4302547.4518671/94‑1 (MQ=60)

      TGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCG                                                                                       <  SRR4302547.4035873/94‑1 (MQ=60)

       GCCGCTAATGTTCAGGCTGCACCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGA                                                                                      <  SRR4302547.3352062/94‑1 (MQ=60)

        CCGCTAATGTTCAGTTTGCAACTGTCATTAAATAGGGTGCCGATGCCAATCAGCTCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGAT                                                                                     <  SRR4302547.6762962/94‑1 (MQ=60)

        CCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGAT                                                                                     <  SRR4302547.1799893/94‑1 (MQ=60)

         CGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATG                                                                                    >  SRR4302547.3033598/1‑94 (MQ=60)

         CGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATG                                                                                    >  SRR4302547.2897669/1‑94 (MQ=60)

          GCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGG                                                                                   >  SRR4302547.5291303/1‑94 (MQ=60)

          GCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGG                                                                                   <  SRR4302547.4777686/94‑1 (MQ=60)

          GCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGG                                                                                   >  SRR4302547.4699788/1‑94 (MQ=60)

           CTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGT                                                                                  >  SRR4302547.5707274/1‑94 (MQ=60)

           CTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGT                                                                                  >  SRR4302547.6223006/1‑94 (MQ=60)

           CTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGT                                                                                  <  SRR4302547.4917333/94‑1 (MQ=60)

            TAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTT                                                                                 <  SRR4302547.56030/94‑1 (MQ=60)

            TAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTT                                                                                 <  SRR4302547.4028346/94‑1 (MQ=60)

            TAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTT                                                                                 <  SRR4302547.2564930/94‑1 (MQ=60)

             AATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGGTA                                                                                >  SRR4302547.2453883/1‑94 (MQ=60)

              ATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAA                                                                               <  SRR4302547.3603244/94‑1 (MQ=60)

              ATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAA                                                                               <  SRR4302547.3536999/94‑1 (MQ=60)

               TGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAAT                                                                              >  SRR4302547.2295/1‑94 (MQ=60)

               TGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAAT                                                                              <  SRR4302547.3198732/94‑1 (MQ=60)

               TGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAAT                                                                              <  SRR4302547.2635350/94‑1 (MQ=60)

                 TTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAA                                                                            <  SRR4302547.4022183/94‑1 (MQ=60)

                    AGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAG                                                                         <  SRR4302547.2568912/94‑1 (MQ=60)

                    AGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAG                                                                         >  SRR4302547.2717713/1‑94 (MQ=60)

                     GGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGT                                                                        >  SRR4302547.6732382/1‑94 (MQ=60)

                     GGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGT                                                                        <  SRR4302547.1251938/94‑1 (MQ=60)

                     GGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGT                                                                        <  SRR4302547.5998543/94‑1 (MQ=60)

                     GGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGT                                                                        <  SRR4302547.2286603/94‑1 (MQ=60)

                      GCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTG                                                                       <  SRR4302547.6770871/94‑1 (MQ=60)

                      GCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTG                                                                       <  SRR4302547.2175266/94‑1 (MQ=60)

                      GCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTG                                                                       <  SRR4302547.4996549/94‑1 (MQ=60)

                      GCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTG                                                                       <  SRR4302547.3056791/94‑1 (MQ=60)

                         GCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATT                                                                    >  SRR4302547.5762615/1‑94 (MQ=60)

                         GCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATT                                                                    <  SRR4302547.1917911/94‑1 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGTCTTTGCGCTCTGGCGATGGTTAATAAAAGTGACTT                                                                   >  SRR4302547.4041153/1‑94 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTT                                                                   <  SRR4302547.867862/94‑1 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTT                                                                   <  SRR4302547.6333793/94‑1 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTT                                                                   >  SRR4302547.415382/1‑94 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTT                                                                   >  SRR4302547.1465704/1‑94 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTT                                                                   <  SRR4302547.4429271/94‑1 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTT                                                                   >  SRR4302547.5301938/1‑94 (MQ=60)

                          CAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTT                                                                   >  SRR4302547.4539558/1‑94 (MQ=60)

                           AACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTC                                                                  >  SRR4302547.2166203/1‑94 (MQ=60)

                           AACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTC                                                                  <  SRR4302547.3893183/94‑1 (MQ=60)

                           AACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTC                                                                  <  SRR4302547.78655/94‑1 (MQ=60)

                            ACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCG                                                                 <  SRR4302547.5867183/94‑1 (MQ=60)

                            ACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCG                                                                 <  SRR4302547.1367510/94‑1 (MQ=60)

                            ACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCG                                                                 >  SRR4302547.2774093/1‑94 (MQ=60)

                             CTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGC                                                                <  SRR4302547.2578943/94‑1 (MQ=60)

                              TGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCT                                                               <  SRR4302547.5347790/94‑1 (MQ=60)

                              TGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCT                                                               >  SRR4302547.2507625/1‑94 (MQ=60)

                               GTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTT                                                              <  SRR4302547.3526467/94‑1 (MQ=60)

                               GTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTT                                                              <  SRR4302547.1878051/94‑1 (MQ=60)

                                TCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTT                                                             <  SRR4302547.4285502/94‑1 (MQ=60)

                                TCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTT                                                             <  SRR4302547.6349985/94‑1 (MQ=60)

                                 CATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTG                                                            >  SRR4302547.6752023/1‑94 (MQ=60)

                                 CATTAAATAGGGTGCCGGTGCCAATAAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTG                                                            <  SRR4302547.4474796/94‑1 (MQ=60)

                                        TAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGG                                                     <  SRR4302547.6495229/94‑1 (MQ=60)

                                         AGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGC                                                    >  SRR4302547.4067063/1‑94 (MQ=60)

                                         AGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGC                                                    >  SRR4302547.3028812/1‑94 (MQ=60)

                                          GGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCT                                                   <  SRR4302547.1793296/94‑1 (MQ=60)

                                               CCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACT                                              >  SRR4302547.1977539/1‑94 (MQ=60)

                                                CGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTG                                             >  SRR4302547.3714854/1‑94 (MQ=60)

                                                CGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTG                                             >  SRR4302547.4755043/1‑94 (MQ=60)

                                                  GTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTT                                           <  SRR4302547.3867552/94‑1 (MQ=60)

                                                   TGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTC                                          >  SRR4302547.826438/1‑94 (MQ=60)

                                                   TGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTC                                          <  SRR4302547.1964871/94‑1 (MQ=60)

                                                      CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTT                                       >  SRR4302547.5928496/1‑94 (MQ=60)

                                                      CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTT                                       >  SRR4302547.1066617/1‑94 (MQ=60)

                                                      CAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTT                                       <  SRR4302547.274563/94‑1 (MQ=60)

                                                       AATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTA                                      >  SRR4302547.6007034/1‑94 (MQ=60)

                                                        ATCAGCGCCAGCGGGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAA                                     >  SRR4302547.1150392/1‑94 (MQ=60)

                                                        ATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAA                                     >  SRR4302547.5728308/1‑94 (MQ=60)

                                                        ATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAA                                     <  SRR4302547.843671/94‑1 (MQ=60)

                                                        ATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAA                                     <  SRR4302547.842663/94‑1 (MQ=60)

                                                        ATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAA                                     >  SRR4302547.2039276/1‑94 (MQ=60)

                                                           AGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTT                                  >  SRR4302547.4861413/1‑94 (MQ=60)

                                                           AGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTT                                  <  SRR4302547.5101995/94‑1 (MQ=60)

                                                             CGCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGCGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTGC                                >  SRR4302547.4806474/1‑94 (MQ=60)

                                                              GCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCC                               <  SRR4302547.425260/94‑1 (MQ=60)

                                                              GCCAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCC                               >  SRR4302547.5498891/1‑94 (MQ=60)

                                                                CAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCAC                             >  SRR4302547.3689035/1‑94 (MQ=60)

                                                                CAGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGGTCTTTAAGTTCCCCC                             >  SRR4302547.2920554/1‑94 (MQ=60)

                                                                 AGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACG                            <  SRR4302547.2205409/94‑1 (MQ=60)

                                                                 AGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACG                            <  SRR4302547.4162447/94‑1 (MQ=60)

                                                                 AGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACG                            <  SRR4302547.4212229/94‑1 (MQ=60)

                                                                 AGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACG                            <  SRR4302547.1683824/94‑1 (MQ=60)

                                                                 AGCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACG                            >  SRR4302547.3175086/1‑94 (MQ=60)

                                                                  GCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGC                           >  SRR4302547.3912436/1‑94 (MQ=60)

                                                                  GCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGC                           <  SRR4302547.2010674/94‑1 (MQ=60)

                                                                  GCGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTACCACTGGCTGCACTGTTCTTTAAGTTCCCACGC                           >  SRR4302547.4002730/1‑94 (MQ=60)

                                                                   CGAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCC                          <  SRR4302547.6757059/94‑1 (MQ=60)

                                                                    GAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCG                         >  SRR4302547.5319871/1‑94 (MQ=60)

                                                                    GAGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCG                         <  SRR4302547.808812/94‑1 (MQ=60)

                                                                     AGCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGG                        >  SRR4302547.3182398/1‑94 (MQ=60)

                                                                      GCTTTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGA                       >  SRR4302547.5303591/1‑94 (MQ=60)

                                                                         TTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGA                    <  SRR4302547.3174532/94‑1 (MQ=60)

                                                                         TTTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGA                    <  SRR4302547.2318886/94‑1 (MQ=60)

                                                                          TTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAG                   >  SRR4302547.1734348/1‑94 (MQ=60)

                                                                          TTCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAG                   <  SRR4302547.5626490/94‑1 (MQ=60)

                                                                           TCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGC                  <  SRR4302547.5443872/94‑1 (MQ=60)

                                                                           TCGCATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGC                  >  SRR4302547.5339696/1‑94 (MQ=60)

                                                                              CATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATG               >  SRR4302547.5702498/1‑94 (MQ=60)

                                                                              CATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATG               >  SRR4302547.2584463/1‑94 (MQ=60)

                                                                               ATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGT              >  SRR4302547.4387928/1‑94 (MQ=60)

                                                                               ATCTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGT              >  SRR4302547.6523037/1‑94 (MQ=60)

                                                                                 CTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAG            >  SRR4302547.2042674/1‑94 (MQ=60)

                                                                                 CTGCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAG            >  SRR4302547.2776135/1‑94 (MQ=60)

                                                                                   GCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGAC          >  SRR4302547.3693156/1‑94 (MQ=60)

                                                                                   GCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGAC          <  SRR4302547.3661264/94‑1 (MQ=60)

                                                                                   GCCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGAC          <  SRR4302547.4784906/94‑1 (MQ=60)

                                                                                    CCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACC         <  SRR4302547.2640376/94‑1 (MQ=60)

                                                                                    CCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACC         <  SRR4302547.839703/94‑1 (MQ=60)

                                                                                    CCTTTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACC         >  SRR4302547.1522016/1‑94 (MQ=60)

                                                                                       TTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACTAGCATGTAGACCGTT      <  SRR4302547.7036/94‑1 (MQ=60)

                                                                                       TTGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTT      >  SRR4302547.6547978/1‑94 (MQ=60)

                                                                                        TGCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTCCCCACGCCGGACGAGCATGTAGACCGTTA     <  SRR4302547.4138738/94‑1 (MQ=60)

                                                                                         GCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAG    <  SRR4302547.6782052/94‑1 (MQ=60)

                                                                                         GCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAG    <  SRR4302547.6229167/94‑1 (MQ=60)

                                                                                         GCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAG    <  SRR4302547.623248/94‑1 (MQ=60)

                                                                                         GCGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAG    >  SRR4302547.6581914/1‑94 (MQ=60)

                                                                                          CGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGG   >  SRR4302547.5972136/1‑94 (MQ=60)

                                                                                          CGCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGG   <  SRR4302547.4945784/94‑1 (MQ=60)

                                                                                           GCTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGC  >  SRR4302547.3648500/1‑94 (MQ=60)

CATCGCTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGC  >  NC_000913/2502292‑2502476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`