Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,515,923	A→G	intergenic (‑71/+138)	b2395 ← / ← alaX	hypothetical protein/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,515,923	0	A	G	88.2%	646.0 / 68.2	229	intergenic (‑71/+138)	b2395/alaX	hypothetical protein/tRNA‑Ala
Reads supporting (aligned to +/- strand): ref base A (13/14); new base G (100/102); total (113/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-01

Only 100 of 242 total aligned reads displayed.

AGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAA  >  NC_000913/2515833‑2516015

  gTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAgg                                                                                          >  2:6093099/1‑93 (MQ=255)

  gTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAgg                                                                                          <  1:2781573/93‑1 (MQ=255)

   ttATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAggg                                                                                         <  1:4317934/93‑1 (MQ=255)

   ttATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAggg                                                                                         <  2:1648424/93‑1 (MQ=255)

   ttATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAggg                                                                                         <  2:2815810/93‑1 (MQ=255)

   ttATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAggg                                                                                         <  1:2469069/93‑1 (MQ=255)

      tGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGc                                                                                      >  2:4975457/1‑93 (MQ=255)

      tGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGc                                                                                      >  2:3129722/1‑93 (MQ=255)

       gCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGcc                                                                                     <  2:5262081/93‑1 (MQ=255)

        cTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCa                                                                                    >  1:4064138/1‑93 (MQ=255)

        cTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCa                                                                                    >  2:2236944/1‑93 (MQ=255)

         tCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAg                                                                                   >  2:2186125/1‑93 (MQ=255)

          ccACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGc                                                                                  <  2:966828/93‑1 (MQ=255)

           cacaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGcc                                                                                 <  2:6443587/93‑1 (MQ=255)

            acaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCt                                                                                <  2:1730167/93‑1 (MQ=255)

               aaCATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGtt                                                                             <  2:3164314/93‑1 (MQ=255)

               aaCATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGtt                                                                             >  2:2676396/1‑93 (MQ=255)

                aCATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTg                                                                            <  2:3199687/93‑1 (MQ=255)

                aCATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTg                                                                            <  2:4098861/93‑1 (MQ=255)

                 cATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGc                                                                           >  2:5811408/1‑93 (MQ=255)

                 cATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGc                                                                           <  1:4619957/93‑1 (MQ=255)

                    gAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGa                                                                        >  2:2527615/1‑93 (MQ=255)

                    gAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGa                                                                        >  2:952148/1‑93 (MQ=255)

                     aGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGAt                                                                       <  1:5375540/93‑1 (MQ=255)

                      gTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGAtt                                                                      >  1:5224683/1‑93 (MQ=255)

                        acacTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGAtttt                                                                    >  2:2343078/1‑93 (MQ=255)

                         cacTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGAttttt                                                                   >  2:1950351/1‑93 (MQ=255)

                            tactCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCAt                                                                >  2:1353911/1‑93 (MQ=255)

                            tactCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCAt                                                                >  1:2923849/1‑93 (MQ=255)

                              ctctCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATcc                                                              >  1:6123554/1‑93 (MQ=255)

                              ctctCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATcc                                                              <  1:3850144/93‑1 (MQ=255)

                               tctcACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCa                                                             <  1:4567966/93‑1 (MQ=255)

                               tctcACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCa                                                             >  2:273706/1‑93 (MQ=255)

                                 tcACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAat                                                           >  1:5732584/1‑93 (MQ=255)

                                 tcACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAat                                                           <  2:5105350/93‑1 (MQ=255)

                                   aCTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGATGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAatga                                                         <  2:4664878/93‑1 (MQ=255)

                                   aCTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAatga                                                         >  1:3271490/1‑93 (MQ=255)

                                   aCTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAatga                                                         <  2:3948248/93‑1 (MQ=255)

                                    cTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAatgat                                                        <  1:180222/93‑1 (MQ=255)

                                    cTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAatgat                                                        >  1:4806778/1‑93 (MQ=255)

                                     tGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAatgatg                                                       >  1:4998825/1‑93 (MQ=255)

                                       aTCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCt                                                     <  2:5887691/93‑1 (MQ=255)

                                       aTCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCt                                                     <  1:4934658/93‑1 (MQ=255)

                                        tCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCtt                                                    >  2:5713403/1‑93 (MQ=255)

                                         cGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCttt                                                   <  2:1878468/93‑1 (MQ=255)

                                          ggggTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTc                                                  >  1:3736704/1‑93 (MQ=255)

                                          ggggCTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTc                                                  >  2:339729/1‑93 (MQ=255)

                                           gggTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCa                                                 >  1:3291461/1‑93 (MQ=255)

                                            ggTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAc                                                >  1:2110265/1‑93 (MQ=255)

                                            ggTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAc                                                <  1:1172847/93‑1 (MQ=255)

                                             gTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAcc                                               <  1:3447700/93‑1 (MQ=255)

                                             gTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAcc                                               <  1:2225560/93‑1 (MQ=255)

                                              ttttCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCa                                              >  1:6415164/1‑93 (MQ=255)

                                                ttCAGTCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAgg                                            <  1:4128041/93‑1 (MQ=255)

                                                 tCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAggc                                           <  1:2499426/93‑1 (MQ=255)

                                                  cAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAggcg                                          <  2:4190995/93‑1 (MQ=255)

                                                   aGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAggcgg                                         >  2:5910929/1‑93 (MQ=255)

                                                   aGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAggcgg                                         >  1:1484525/1‑93 (MQ=255)

                                                   aGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAggcgg                                         >  1:3199974/1‑93 (MQ=255)

                                                      cacacaAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCAc                                      <  2:398223/93‑1 (MQ=255)

                                                      cacacaAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCAc                                      <  2:625570/93‑1 (MQ=255)

                                                      cacacaAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCAc                                      <  1:1093146/93‑1 (MQ=255)

                                                        cacaAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTa                                    >  1:4362355/1‑93 (MQ=255)

                                                        cacaAATAATAAGTTTAGTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTa                                    <  2:2537986/93‑1 (MQ=255)

                                                           aaaTAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                 >  1:2993961/1‑93 (MQ=255)

                                                           aaaTAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                 <  1:2806499/93‑1 (MQ=255)

                                                           aaaTAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                 <  2:672263/93‑1 (MQ=255)

                                                            aataatAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAccc                                >  1:2685621/1‑93 (MQ=255)

                                                            aataatAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAccc                                >  1:6336607/1‑93 (MQ=255)

                                                             ataataAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCg                               <  1:3390403/93‑1 (MQ=255)

                                                             ataataAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCg                               <  1:6149652/93‑1 (MQ=255)

                                                              taataaGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCgg                              >  1:5851847/1‑93 (MQ=255)

                                                               aataaGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGa                             <  1:3139862/93‑1 (MQ=255)

                                                               aataaGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGa                             <  1:5811408/93‑1 (MQ=255)

                                                                ataaGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGAc                            >  1:3887950/1‑93 (MQ=255)

                                                                  aaGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGt                          <  1:1050497/93‑1 (MQ=255)

                                                                   aGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTa                         >  2:5184388/1‑93 (MQ=255)

                                                                   aGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTa                         <  2:4396213/93‑1 (MQ=255)

                                                                     tttATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                       <  2:2959921/93‑1 (MQ=255)

                                                                      ttATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGAc                      <  1:599643/93‑1 (MQ=255)

                                                                      ttATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGAc                      <  2:6022190/93‑1 (MQ=255)

                                                                      ttATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGAc                      <  2:4854951/93‑1 (MQ=255)

                                                                         ttCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGt                   >  2:3149272/1‑93 (MQ=255)

                                                                          tCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTa                  >  1:5598352/1‑93 (MQ=255)

                                                                           cGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTAt                 >  2:3831007/1‑93 (MQ=255)

                                                                           cGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTAt                 <  2:6168538/93‑1 (MQ=255)

                                                                            gCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTAtt                >  2:474684/1‑93 (MQ=255)

                                                                             cATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTa               >  2:4267758/1‑93 (MQ=255)

                                                                               tCGCGAAGCAAGCAGGGGGCCCGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACa             >  2:1247102/1‑93 (MQ=255)

                                                                               tCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACa             >  2:3446437/1‑93 (MQ=255)

                                                                               tCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACa             >  1:1369849/1‑93 (MQ=255)

                                                                                 gcgAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACaaa           >  2:2002391/1‑93 (MQ=255)

                                                                                 gcgAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACaaa           >  2:4913356/1‑93 (MQ=255)

                                                                                 gcgAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACaaa           >  2:3220446/1‑93 (MQ=255)

                                                                                 gcgAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACaaa           >  2:6538442/1‑93 (MQ=255)

                                                                                   gAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAg         <  1:3252424/93‑1 (MQ=255)

                                                                                   gAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAg         >  1:1653780/1‑93 (MQ=255)

                                                                                    aagcaagcAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGc        >  2:1038513/1‑93 (MQ=255)

                                                                                     agcaagcaGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTACCCCGGACGTAGACTGTATTACAAAAGCg       <  1:1062023/93‑1 (MQ=255)

                                                                                          aCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCaaa  >  1:5476951/1‑93 (MQ=255)

AGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAA  >  NC_000913/2515833‑2516015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAA  >  NC_000913/2515832‑2516015

CAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCA                                                                                            >  SRR4302547.628455/1‑94 (MQ=60)

CAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCA                                                                                            <  SRR4302547.5039238/94‑1 (MQ=60)

CAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCA                                                                                            <  SRR4302547.5124770/94‑1 (MQ=60)

 AGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAG                                                                                           <  SRR4302547.3269250/94‑1 (MQ=60)

   GTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGG                                                                                         <  SRR4302547.2837293/94‑1 (MQ=60)

   GTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGG                                                                                         <  SRR4302547.1096297/94‑1 (MQ=60)

    TTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGG                                                                                        <  SRR4302547.4406807/94‑1 (MQ=60)

    TTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGG                                                                                        <  SRR4302547.2518975/94‑1 (MQ=60)

    TTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGG                                                                                        >  SRR4302547.4920258/1‑94 (MQ=60)

     TATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGG                                                                                       <  SRR4302547.3625994/94‑1 (MQ=60)

        GCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCA                                                                                    >  SRR4302547.4146682/1‑94 (MQ=60)

           CCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCC                                                                                 >  SRR4302547.5791793/1‑94 (MQ=60)

            CACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCT                                                                                <  SRR4302547.1580266/94‑1 (MQ=60)

               AAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTT                                                                             <  SRR4302547.3988733/94‑1 (MQ=60)

               AAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTT                                                                             <  SRR4302547.5044701/94‑1 (MQ=60)

               AAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTT                                                                             <  SRR4302547.2870314/94‑1 (MQ=60)

                AACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTG                                                                            <  SRR4302547.6463294/94‑1 (MQ=60)

                  CATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCC                                                                          <  SRR4302547.4715621/94‑1 (MQ=60)

                      AGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATT                                                                      <  SRR4302547.5486492/94‑1 (MQ=60)

                      AGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATT                                                                      >  SRR4302547.5332229/1‑94 (MQ=60)

                       GTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTT                                                                     <  SRR4302547.5927503/94‑1 (MQ=60)

                       GTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTT                                                                     <  SRR4302547.1812153/94‑1 (MQ=60)

                        TACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTT                                                                    >  SRR4302547.3953425/1‑94 (MQ=60)

                            CTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCAT                                                                >  SRR4302547.2982149/1‑94 (MQ=60)

                            CTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCAT                                                                >  SRR4302547.3396348/1‑94 (MQ=60)

                             TACTCTCACTGATCAGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATC                                                               >  SRR4302547.296511/1‑94 (MQ=60)

                              ACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCC                                                              >  SRR4302547.6250587/1‑94 (MQ=60)

                              ACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCC                                                              <  SRR4302547.3084623/94‑1 (MQ=60)

                               CTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCA                                                             <  SRR4302547.3928527/94‑1 (MQ=60)

                               CTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCA                                                             >  SRR4302547.2521941/1‑94 (MQ=60)

                                TCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAA                                                            <  SRR4302547.4662489/94‑1 (MQ=60)

                                TCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAA                                                            >  SRR4302547.5876029/1‑94 (MQ=60)

                                 CTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAAT                                                           >  SRR4302547.5851018/1‑94 (MQ=60)

                                 CTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAAT                                                           <  SRR4302547.4494926/94‑1 (MQ=60)

                                  TCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCTGCTTTTTCATCCcatc                                                          >  SRR4302547.3181929/1‑90 (MQ=60)

                                  TCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATG                                                          >  SRR4302547.4422479/1‑94 (MQ=60)

                                  TCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATG                                                          >  SRR4302547.1076572/1‑94 (MQ=60)

                                   CACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGA                                                         >  SRR4302547.3336989/1‑94 (MQ=60)

                                   CACTGATCGGGGGTTTCAGGCACACGAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGA                                                         >  SRR4302547.6048550/1‑94 (MQ=60)

                                    ACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGAT                                                        >  SRR4302547.6275839/1‑94 (MQ=60)

                                    ACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGAT                                                        >  SRR4302547.2676052/1‑94 (MQ=60)

                                    ACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGAT                                                        <  SRR4302547.1427910/94‑1 (MQ=60)

                                    ACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGAT                                                        >  SRR4302547.4906394/1‑94 (MQ=60)

                                    ACTGATCGGGGTTTTCAGGCACACAAATAATAAGTGTATTCGCATCGCGAAACAACCAGGGGGCCACCCTGTTGCCGATTTTTCATCCAATGAT                                                        <  SRR4302547.2195764/94‑1 (MQ=60)

                                     CTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATG                                                       <  SRR4302547.3884882/94‑1 (MQ=60)

                                     CTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATG                                                       >  SRR4302547.5102176/1‑94 (MQ=60)

                                     CTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATG                                                       <  SRR4302547.183603/94‑1 (MQ=60)

                                       GATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCT                                                     >  SRR4302547.5443164/1‑94 (MQ=60)

                                        ATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTT                                                    <  SRR4302547.5036887/94‑1 (MQ=60)

                                        ATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGCCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTT                                                    <  SRR4302547.2230417/94‑1 (MQ=60)

                                         TCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTT                                                   >  SRR4302547.3521020/1‑94 (MQ=60)

                                          TGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTC                                                  >  SRR4302547.3812994/1‑94 (MQ=60)

                                          CGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGTGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTC                                                  <  SRR4302547.6555176/94‑1 (MQ=60)

                                          CGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTC                                                  <  SRR4302547.6102159/94‑1 (MQ=60)

                                          CGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTC                                                  <  SRR4302547.3038320/94‑1 (MQ=60)

                                           GGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCA                                                 >  SRR4302547.3357553/1‑94 (MQ=60)

                                           GGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCA                                                 <  SRR4302547.559568/94‑1 (MQ=60)

                                            GGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAC                                                >  SRR4302547.2152305/1‑94 (MQ=60)

                                            GGGTTTTCAGGCACACAAATAATAAGTCTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAC                                                >  SRR4302547.2708494/1‑94 (MQ=60)

                                             GGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACC                                               <  SRR4302547.1196551/94‑1 (MQ=60)

                                             GGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACC                                               <  SRR4302547.2862101/94‑1 (MQ=60)

                                              GTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCA                                              <  SRR4302547.3517985/94‑1 (MQ=60)

                                              GTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCA                                              >  SRR4302547.6548935/1‑94 (MQ=60)

                                              GTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCA                                              <  SRR4302547.2270406/94‑1 (MQ=60)

                                                TTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGG                                            <  SRR4302547.2353038/94‑1 (MQ=60)

                                                TTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGG                                            <  SRR4302547.480350/94‑1 (MQ=60)

                                                 TTCAGTCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGC                                           <  SRR4302547.4212002/94‑1 (MQ=60)

                                                  TCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCG                                          <  SRR4302547.2549921/94‑1 (MQ=60)

                                                   CAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGG                                         >  SRR4302547.1514142/1‑94 (MQ=60)

                                                   CAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGG                                         >  SRR4302547.3263771/1‑94 (MQ=60)

                                                       CACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACT                                     <  SRR4302547.4975476/94‑1 (MQ=60)

                                                       CACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACT                                     <  SRR4302547.1115062/94‑1 (MQ=60)

                                                        ACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTA                                    >  SRR4302547.3334010/1‑94 (MQ=60)

                                                        ACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTA                                    >  SRR4302547.3237149/1‑94 (MQ=60)

                                                        ACACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTA                                    >  SRR4302547.4451944/1‑94 (MQ=60)

                                                         CACAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAA                                   <  SRR4302547.4319009/94‑1 (MQ=60)

                                                           CAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACC                                 <  SRR4302547.5665593/94‑1 (MQ=60)

                                                           CAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACC                                 >  SRR4302547.5181979/1‑94 (MQ=60)

                                                           CAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACC                                 <  SRR4302547.1126144/94‑1 (MQ=60)

                                                           CAAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACC                                 >  SRR4302547.3053534/1‑94 (MQ=60)

                                                            AAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCC                                >  SRR4302547.2739528/1‑94 (MQ=60)

                                                            AAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCC                                >  SRR4302547.6468546/1‑94 (MQ=60)

                                                            AAATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCC                                <  SRR4302547.2862668/94‑1 (MQ=60)

                                                              ATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGG                              <  SRR4302547.3459378/94‑1 (MQ=60)

                                                              ATAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGG                              <  SRR4302547.6277241/94‑1 (MQ=60)

                                                              ATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGG                              >  SRR4302547.5972892/1‑94 (MQ=60)

                                                               TAATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGA                             >  SRR4302547.452246/1‑94 (MQ=60)

                                                                AATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGAC                            >  SRR4302547.3967036/1‑94 (MQ=60)

                                                                AATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGAC                            <  SRR4302547.3202241/94‑1 (MQ=60)

                                                                AATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGAC                            <  SRR4302547.5931644/94‑1 (MQ=60)

                                                                 ATAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACG                           <  SRR4302547.3991668/94‑1 (MQ=60)

                                                                 ATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACG                           >  SRR4302547.4677184/1‑94 (MQ=60)

                                                                  TAAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGT                          <  SRR4302547.138315/94‑1 (MQ=60)

                                                                   AAGTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTA                         <  SRR4302547.1071323/94‑1 (MQ=60)

                                                                     GTTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGA                       >  SRR4302547.331841/1‑94 (MQ=60)

                                                                     ATTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGA                       <  SRR4302547.618168/94‑1 (MQ=60)

                                                                      TTTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGAC                      <  SRR4302547.1924714/94‑1 (MQ=60)

                                                                       TTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACT                     <  SRR4302547.610707/94‑1 (MQ=60)

                                                                       TTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACT                     >  SRR4302547.4360273/1‑94 (MQ=60)

                                                                       TTATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACT                     >  SRR4302547.3337363/1‑94 (MQ=60)

                                                                        TATTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTG                    >  SRR4302547.237337/1‑94 (MQ=60)

                                                                          TTCGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTA                  >  SRR4302547.5713833/1‑94 (MQ=60)

                                                                            CGCATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATT                <  SRR4302547.1862595/94‑1 (MQ=60)

                                                                              CATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTAC              >  SRR4302547.5961645/1‑94 (MQ=60)

                                                                              CATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTAC              <  SRR4302547.1425313/94‑1 (MQ=60)

                                                                               ATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACA             >  SRR4302547.1397338/1‑94 (MQ=60)

                                                                               ATCGCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACA             >  SRR4302547.6476/1‑94 (MQ=60)

                                                                                  GCGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAA          >  SRR4302547.3072815/1‑94 (MQ=60)

                                                                                  GCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAA          >  SRR4302547.2784164/1‑94 (MQ=60)

                                                                                   CGAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAG         >  SRR4302547.1686410/1‑94 (MQ=60)

                                                                                    GAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGC        >  SRR4302547.4202008/1‑94 (MQ=60)

                                                                                    GAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGC        >  SRR4302547.5565139/1‑94 (MQ=60)

                                                                                    GAAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGC        <  SRR4302547.3317406/94‑1 (MQ=60)

                                                                                     AAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCG       >  SRR4302547.1159722/1‑94 (MQ=60)

                                                                                     AAGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCG       <  SRR4302547.3466251/94‑1 (MQ=60)

                                                                                      AGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTACCCCGGACGTAGACTGTATTACAAAAGCGG      <  SRR4302547.1083157/94‑1 (MQ=60)

                                                                                      AGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGG      >  SRR4302547.521520/1‑94 (MQ=60)

                                                                                      AGCAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGG      >  SRR4302547.3384968/1‑94 (MQ=60)

                                                                                        CAAGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCA    >  SRR4302547.1081030/1‑94 (MQ=60)

                                                                                          AGCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAA  <  SRR4302547.6546085/94‑1 (MQ=60)

                                                                                          AACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAA  >  SRR4302547.5590066/1‑94 (MQ=60)

CAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAA  >  NC_000913/2515832‑2516015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`