Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,586,147 A→G Y178C (TAT→TGT)  acrD → AcrD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,586,1470AG87.9% 720.4 / 76.5 257Y178C (TAT→TGT) acrDAcrD
Reads supporting (aligned to +/- strand):  ref base A (16/15);  new base G (111/115);  total (127/130)
Fisher's exact test for biased strand distribution p-value = 8.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 263 total aligned reads displayed.

TTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAG  >  NC_000913/2586055‑2586238
                                                                                            |                                                                                           
ttCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTa                                                                                             >  1:3564224/1‑93 (MQ=255)
 tCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTAt                                                                                            >  1:4493216/1‑93 (MQ=255)
 tCGATGGATAAACAGAATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTAt                                                                                            <  2:3396022/93‑1 (MQ=255)
  cGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTAAAGCCGCGTGAATGGCGTCGGGGATATCGCTGCCTATg                                                                                           <  1:6505505/93‑1 (MQ=255)
   gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTgg                                                                                          <  1:4875225/93‑1 (MQ=255)
   gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTgg                                                                                          >  1:5042078/1‑93 (MQ=255)
   gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTgg                                                                                          <  2:5561125/93‑1 (MQ=255)
    aTGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGt                                                                                         <  1:626979/93‑1 (MQ=255)
    aTGGATAAACAGGATATTGATGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGt                                                                                         >  1:6482098/1‑93 (MQ=255)
     tGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGtt                                                                                        >  2:1740324/1‑93 (MQ=255)
     tGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGtt                                                                                        >  2:490531/1‑93 (MQ=255)
       gATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCg                                                                                      >  2:5804574/1‑93 (MQ=255)
         tAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCa                                                                                    >  1:6618696/1‑93 (MQ=255)
          aaaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCaa                                                                                   <  1:17087/93‑1 (MQ=255)
           aaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAat                                                                                  <  1:5940835/93‑1 (MQ=255)
           aaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAat                                                                                  <  1:3621750/93‑1 (MQ=255)
           aaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAat                                                                                  >  2:1905944/1‑93 (MQ=255)
           aaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAat                                                                                  >  2:2372313/1‑93 (MQ=255)
             cAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAatat                                                                                >  2:6475274/1‑93 (MQ=255)
              aGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATAtt                                                                               >  1:2258342/1‑93 (MQ=255)
               ggATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTc                                                                              <  1:6068043/93‑1 (MQ=255)
               ggATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTc                                                                              >  2:5359858/1‑93 (MQ=255)
               ggATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTc                                                                              <  2:5662010/93‑1 (MQ=255)
                gATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTcc                                                                             <  2:5197920/93‑1 (MQ=255)
                 atatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCa                                                                            <  1:3445917/93‑1 (MQ=255)
                  tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTcctg                                                                           >  2:128080/1‑91 (MQ=255)
                   atTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATg                                                                          <  2:1394035/93‑1 (MQ=255)
                   atTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATg                                                                          >  1:5779014/1‑93 (MQ=255)
                   atTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATg                                                                          >  2:1958099/1‑93 (MQ=255)
                     tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCg                                                                        >  1:2644505/1‑93 (MQ=255)
                      gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGt                                                                       <  2:2675011/93‑1 (MQ=255)
                       gtGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTa                                                                      <  2:319655/92‑1 (MQ=255)
                        tGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGCTCGCAATATTCCATGCGTAt                                                                     >  1:1020658/1‑93 (MQ=255)
                           ttATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATctg                                                                  >  2:2222966/1‑93 (MQ=255)
                              tGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGCTATCGATGCCTGTGGTTCGCAATATTCCATGCGTATctggct                                                               <  2:4669106/93‑1 (MQ=255)
                              tGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATctggct                                                               <  1:4707081/93‑1 (MQ=255)
                               gTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATctggctg                                                              >  2:148451/1‑93 (MQ=255)
                                ttGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATctggctgg                                                             >  2:4715936/1‑93 (MQ=255)
                                 tGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGa                                                            >  1:245514/1‑93 (MQ=255)
                                   ccAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGAcc                                                          <  2:4820846/93‑1 (MQ=255)
                                   ccAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGAcc                                                          <  1:384413/93‑1 (MQ=255)
                                    cAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGAccc                                                         <  2:588106/93‑1 (MQ=255)
                                     aGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCg                                                        <  2:4298324/93‑1 (MQ=255)
                                      gTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCgg                                                       <  2:6636038/93‑1 (MQ=255)
                                       tAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGc                                                      <  2:5503615/93‑1 (MQ=255)
                                       tAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGc                                                      >  1:4632336/1‑93 (MQ=255)
                                        aaTATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCg                                                     >  2:2442174/1‑93 (MQ=255)
                                            ttCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAAc                                                 <  2:3370504/93‑1 (MQ=255)
                                              cAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTc                                               >  2:2750049/1‑93 (MQ=255)
                                                ggACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCaa                                             >  1:5615823/1‑93 (MQ=255)
                                                ggACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCCATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCac                                             >  2:3312543/1‑92 (MQ=255)
                                                 gACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAAc                                            <  1:2765236/93‑1 (MQ=255)
                                                  gcccGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACa                                           <  2:4535245/92‑1 (MQ=255)
                                                  aCCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACa                                           >  1:1265474/1‑93 (MQ=255)
                                                  aCCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACa                                           <  1:1338404/93‑1 (MQ=255)
                                                   cccGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAg                                          <  2:1675357/93‑1 (MQ=255)
                                                   cccGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAg                                          >  1:800123/1‑93 (MQ=255)
                                                   cccGGTAAGCCGCGTGAATGGCGTCGGGGATATCGATTCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAg                                          <  2:6338847/93‑1 (MQ=255)
                                                     cGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGtt                                        <  2:3639757/93‑1 (MQ=255)
                                                     cGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGtt                                        <  1:4005674/93‑1 (MQ=255)
                                                      gTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGTAACTCAACAGttt                                       <  1:6381346/93‑1 (MQ=255)
                                                       ttAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTc                                      >  2:3123207/1‑93 (MQ=255)
                                                          aGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAg                                   <  1:2497334/93‑1 (MQ=255)
                                                           gCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGa                                  >  1:3255045/1‑93 (MQ=255)
                                                            ccGCGTGAGTGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGAt                                 <  1:2338121/93‑1 (MQ=255)
                                                            ccGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTGCAGAt                                 >  1:573485/1‑93 (MQ=255)
                                                            ccGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGAt                                 <  1:1423773/93‑1 (MQ=255)
                                                             cgcgTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATg                                >  1:2814091/1‑93 (MQ=255)
                                                                 tGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACgg                            >  1:2077247/1‑93 (MQ=255)
                                                                 tGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACgg                            >  2:6184651/1‑93 (MQ=255)
                                                                  gAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGc                           <  2:1573195/93‑1 (MQ=255)
                                                                   aaTGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCt                          <  1:1766775/93‑1 (MQ=255)
                                                                    aTGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTa                         >  1:3019515/1‑93 (MQ=255)
                                                                      ggCGTCGTGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTaaa                       <  2:3689004/93‑1 (MQ=255)
                                                                       gCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCTAGATGACGGCTgaaa                      >  2:6591308/1‑92 (MQ=255)
                                                                        cGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGa                     <  2:6061065/93‑1 (MQ=255)
                                                                        cGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGa                     >  1:1695384/1‑93 (MQ=255)
                                                                        cGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGa                     <  1:4827348/93‑1 (MQ=255)
                                                                         gTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGAt                    <  2:6106901/93‑1 (MQ=255)
                                                                          tCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATg                   >  2:3996812/1‑93 (MQ=255)
                                                                          tCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATg                   >  1:4333331/1‑93 (MQ=255)
                                                                          tCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATg                   >  1:194684/1‑93 (MQ=255)
                                                                            ggggATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTc                 <  1:4947519/93‑1 (MQ=255)
                                                                            ggggATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTc                 <  2:4426465/93‑1 (MQ=255)
                                                                            ggggATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTc                 <  1:4075357/93‑1 (MQ=255)
                                                                            ggggAGATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTc                 <  2:5071477/93‑1 (MQ=255)
                                                                             gggATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCa                >  2:2930163/1‑93 (MQ=255)
                                                                             gggATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCa                >  1:5162085/1‑93 (MQ=255)
                                                                               gATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt              <  2:2941284/93‑1 (MQ=255)
                                                                               gATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACt              >  1:1001101/1‑93 (MQ=255)
                                                                                atatCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg             <  1:1982891/93‑1 (MQ=255)
                                                                                 tatCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGa            <  2:834064/93‑1 (MQ=255)
                                                                                 tatCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGa            <  2:1119249/93‑1 (MQ=255)
                                                                                    cGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGc         <  2:3441644/93‑1 (MQ=255)
                                                                                      aTGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa       >  1:943748/1‑93 (MQ=255)
                                                                                      aTGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa       >  1:4252182/1‑93 (MQ=255)
                                                                                       tGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGGCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAt      <  2:4990791/93‑1 (MQ=255)
                                                                                       tGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAt      >  2:2608951/1‑93 (MQ=255)
                                                                                        tccTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAtt     <  2:6277268/92‑1 (MQ=255)
                                                                                         ccTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTg    >  1:3186068/1‑93 (MQ=255)
                                                                                            |                                                                                           
TTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAG  >  NC_000913/2586055‑2586238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTC  >  NC_000913/2586054‑2586240
                                                                                             |                                                                                             
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTG                                                                                               <  SRR4302547.4362399/94‑1 (MQ=60)
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTG                                                                                               >  SRR4302547.1921813/1‑94 (MQ=60)
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTG                                                                                               >  SRR4302547.4471482/1‑94 (MQ=60)
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTA                                                                                               <  SRR4302547.5742982/94‑1 (MQ=60)
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTA                                                                                               >  SRR4302547.3636952/1‑94 (MQ=60)
 TTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGT                                                                                              <  SRR4302547.2758998/94‑1 (MQ=60)
 TTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTAT                                                                                              >  SRR4302547.4586023/1‑94 (MQ=60)
 GTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGT                                                                                              <  SRR4302547.1565662/94‑1 (MQ=60)
   CGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGG                                                                                            >  SRR4302547.5146198/1‑94 (MQ=60)
   CGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGG                                                                                            >  SRR4302547.5860374/1‑94 (MQ=60)
   CGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGG                                                                                            >  SRR4302547.4957406/1‑94 (MQ=60)
   CGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTAAAGCCGCGTGAATGGCGTCGGGGATATCGCTGCCTATGG                                                                                            <  SRR4302547.6640919/94‑1 (MQ=60)
    GATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGT                                                                                           <  SRR4302547.4976240/94‑1 (MQ=60)
    GATGGATAAACAGGATATTGATGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGT                                                                                           >  SRR4302547.6617049/1‑94 (MQ=60)
     ATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTT                                                                                          >  SRR4302547.644731/1‑94 (MQ=60)
     ATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTT                                                                                          <  SRR4302547.3780997/94‑1 (MQ=60)
     ATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTT                                                                                          <  SRR4302547.638554/94‑1 (MQ=60)
      TGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGTGGATATCGATGCCTGTGGTTC                                                                                         >  SRR4302547.2323578/1‑94 (MQ=60)
       GGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCG                                                                                        <  SRR4302547.1521038/94‑1 (MQ=60)
       GGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCG                                                                                        >  SRR4302547.3448020/1‑94 (MQ=60)
         ATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCA                                                                                      >  SRR4302547.3677501/1‑94 (MQ=60)
         ATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCA                                                                                      >  SRR4302547.6756451/1‑94 (MQ=60)
           AAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAAT                                                                                    <  SRR4302547.17408/94‑1 (MQ=60)
           AAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAAT                                                                                    >  SRR4302547.429495/1‑94 (MQ=60)
            AACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATA                                                                                   <  SRR4302547.6064022/94‑1 (MQ=60)
            AACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATA                                                                                   <  SRR4302547.3695627/94‑1 (MQ=60)
            AACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATA                                                                                   >  SRR4302547.913851/1‑94 (MQ=60)
             ACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATAT                                                                                  >  SRR4302547.1440848/1‑94 (MQ=60)
              CAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGGGTCGGGGATATCGATGCCTGTGGTTCGCAATATT                                                                                 >  SRR4302547.2530132/1‑94 (MQ=60)
              CAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATT                                                                                 <  SRR4302547.87219/94‑1 (MQ=60)
              CAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATT                                                                                 >  SRR4302547.2303956/1‑94 (MQ=60)
               AGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTC                                                                                >  SRR4302547.3188897/1‑94 (MQ=60)
               AGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTC                                                                                >  SRR4302547.5440202/1‑94 (MQ=60)
                GGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCC                                                                               <  SRR4302547.6193900/94‑1 (MQ=60)
                 GATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCA                                                                              >  SRR4302547.2559963/1‑94 (MQ=60)
                  ATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCAT                                                                             <  SRR4302547.5744884/94‑1 (MQ=60)
                  ATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCAT                                                                             <  SRR4302547.3516169/94‑1 (MQ=60)
                  ATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCAT                                                                             >  SRR4302547.3653371/1‑94 (MQ=60)
                   TATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATG                                                                            <  SRR4302547.4445276/94‑1 (MQ=60)
                   TATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATG                                                                            >  SRR4302547.5898550/1‑94 (MQ=60)
                   TATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATG                                                                            >  SRR4302547.78421/1‑94 (MQ=60)
                    ATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGC                                                                           <  SRR4302547.671147/94‑1 (MQ=60)
                    ATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGC                                                                           <  SRR4302547.4744128/94‑1 (MQ=60)
                     TTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCG                                                                          <  SRR4302547.2787882/94‑1 (MQ=60)
                     TTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCG                                                                          >  SRR4302547.2697718/1‑94 (MQ=60)
                      TGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGT                                                                         >  SRR4302547.1415064/1‑94 (MQ=60)
                      TGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGT                                                                         >  SRR4302547.998672/1‑94 (MQ=60)
                       GCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTA                                                                        >  SRR4302547.1170169/1‑94 (MQ=60)
                        CTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGGCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTAT                                                                       >  SRR4302547.6578413/1‑94 (MQ=60)
                        CTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGCTCGCAATATTCCATGCGTAT                                                                       >  SRR4302547.1040667/1‑94 (MQ=60)
                         TGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATC                                                                      >  SRR4302547.5893220/1‑94 (MQ=60)
                         TGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATC                                                                      <  SRR4302547.2753499/94‑1 (MQ=60)
                         TGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATC                                                                      >  SRR4302547.5093956/1‑94 (MQ=60)
                          GATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCT                                                                     <  SRR4302547.385888/94‑1 (MQ=60)
                          GATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCT                                                                     >  SRR4302547.3381876/1‑94 (MQ=60)
                            TTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGG                                                                   <  SRR4302547.6446759/94‑1 (MQ=60)
                            TTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGG                                                                   >  SRR4302547.2475658/1‑94 (MQ=60)
                             TATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGC                                                                  >  SRR4302547.2856956/1‑94 (MQ=60)
                               TGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTG                                                                <  SRR4302547.4804560/94‑1 (MQ=60)
                                GTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGG                                                               <  SRR4302547.3279840/94‑1 (MQ=60)
                                 TTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGA                                                              >  SRR4302547.250100/1‑94 (MQ=60)
                                 TTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGA                                                              <  SRR4302547.4815747/94‑1 (MQ=60)
                                    CCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCC                                                           >  SRR4302547.5968850/1‑94 (MQ=60)
                                    CCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCC                                                           <  SRR4302547.391543/94‑1 (MQ=60)
                                     CAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCG                                                          >  SRR4302547.1298200/1‑94 (MQ=60)
                                     CAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCG                                                          <  SRR4302547.5098521/94‑1 (MQ=60)
                                     CAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCG                                                          <  SRR4302547.176315/94‑1 (MQ=60)
                                      AGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGG                                                         <  SRR4302547.2005168/94‑1 (MQ=60)
                                      AGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGG                                                         >  SRR4302547.4108769/1‑94 (MQ=60)
                                       GTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGC                                                        >  SRR4302547.4728284/1‑94 (MQ=60)
                                        TAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCG                                                       >  SRR4302547.264982/1‑94 (MQ=60)
                                         AATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGA                                                      >  SRR4302547.105592/1‑94 (MQ=60)
                                               CAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTGCGCAGTATTCCATGCGTATCTGGCTGGGCCCGGCGAAACTCA                                                <  SRR4302547.1569985/94‑1 (MQ=60)
                                               CAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCA                                                <  SRR4302547.4386937/94‑1 (MQ=60)
                                               CAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCA                                                >  SRR4302547.5019597/1‑94 (MQ=60)
                                                AGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAA                                               >  SRR4302547.2208040/1‑94 (MQ=60)
                                                AGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAA                                               >  SRR4302547.5731709/1‑94 (MQ=60)
                                                  GACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACA                                             >  SRR4302547.1290964/1‑94 (MQ=60)
                                                  GACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACA                                             <  SRR4302547.2820645/94‑1 (MQ=60)
                                                   ACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAG                                            >  SRR4302547.815276/1‑94 (MQ=60)
                                                   ACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAG                                            <  SRR4302547.1365305/94‑1 (MQ=60)
                                                   ACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGCGGTAACTCAACAG                                            >  SRR4302547.1692203/1‑94 (MQ=60)
                                                     CCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTT                                          >  SRR4302547.6217015/1‑94 (MQ=60)
                                                     CCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTT                                          <  SRR4302547.2112087/94‑1 (MQ=60)
                                                      CGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTT                                         <  SRR4302547.4087090/94‑1 (MQ=60)
                                                      CGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTT                                         >  SRR4302547.1785030/1‑94 (MQ=60)
                                                       GTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGTAACTCAACAGTTTC                                        <  SRR4302547.6514511/94‑1 (MQ=60)
                                                       GTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTC                                        >  SRR4302547.5911216/1‑94 (MQ=60)
                                                        TTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCC                                       <  SRR4302547.3998451/94‑1 (MQ=60)
                                                         TAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCA                                      >  SRR4302547.1027967/1‑94 (MQ=60)
                                                          AAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAG                                     >  SRR4302547.5513867/1‑94 (MQ=60)
                                                           AGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGA                                    >  SRR4302547.3320085/1‑94 (MQ=60)
                                                           AGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGA                                    <  SRR4302547.2547783/94‑1 (MQ=60)
                                                           AGCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGA                                    >  SRR4302547.3537001/1‑94 (MQ=60)
                                                            GCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTGCAGAT                                   >  SRR4302547.583779/1‑94 (MQ=60)
                                                            GCCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCACGCGTATCTGGCTGGACCCGCCGAAACTCAACAGTTTCCAGta                                   >  SRR4302547.746071/1‑92 (MQ=60)
                                                             CCGCGTGAGTGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATG                                  <  SRR4302547.2385522/94‑1 (MQ=60)
                                                             CCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATG                                  <  SRR4302547.6259808/94‑1 (MQ=60)
                                                             CCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATG                                  <  SRR4302547.1452277/94‑1 (MQ=60)
                                                             CCGCGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATG                                  >  SRR4302547.2870385/1‑94 (MQ=60)
                                                             CCGCGTGAAAGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATG                                  >  SRR4302547.6621526/1‑94 (MQ=60)
                                                                CGTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACG                               <  SRR4302547.2096301/94‑1 (MQ=60)
                                                                CGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACG                               >  SRR4302547.4822816/1‑94 (MQ=60)
                                                                 GTGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGG                              >  SRR4302547.2118672/1‑94 (MQ=60)
                                                                  TGAATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGC                             <  SRR4302547.5206535/94‑1 (MQ=60)
                                                                    AATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTA                           <  SRR4302547.1801823/94‑1 (MQ=60)
                                                                    AATGGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTA                           >  SRR4302547.3079558/1‑94 (MQ=60)
                                                                       GGCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAG                        <  SRR4302547.185686/94‑1 (MQ=60)
                                                                        GCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGA                       <  SRR4302547.1738991/94‑1 (MQ=60)
                                                                        GCGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGA                       >  SRR4302547.1728717/1‑94 (MQ=60)
                                                                         CGTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGAT                      <  SRR4302547.4927431/94‑1 (MQ=60)
                                                                          GTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATG                     >  SRR4302547.4422478/1‑94 (MQ=60)
                                                                          GTCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATG                     >  SRR4302547.198323/1‑94 (MQ=60)
                                                                           TCGGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGT                    >  SRR4302547.4122166/1‑94 (MQ=60)
                                                                             GGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCA                  >  SRR4302547.5268375/1‑94 (MQ=60)
                                                                             GGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCA                  <  SRR4302547.4158162/94‑1 (MQ=60)
                                                                             GGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCA                  <  SRR4302547.5049977/94‑1 (MQ=60)
                                                                             GGGGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCA                  <  SRR4302547.5049976/94‑1 (MQ=60)
                                                                             GGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCA                  >  SRR4302547.444117/1‑94 (MQ=60)
                                                                               GGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGGTGTCACT                <  SRR4302547.3038920/94‑1 (MQ=60)
                                                                               GGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACT                <  SRR4302547.4183212/94‑1 (MQ=60)
                                                                               GGATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACT                >  SRR4302547.1020621/1‑94 (MQ=60)
                                                                                GATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTG               <  SRR4302547.6626023/94‑1 (MQ=60)
                                                                                 ATATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGA              <  SRR4302547.2022267/94‑1 (MQ=60)
                                                                                  TATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGAT             <  SRR4302547.2920960/94‑1 (MQ=60)
                                                                                   ATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTTGCTGGACCCGGCGAAACTCAACAGATTCCAGATGACGGCTAAAGATGTCACTGATG            >  SRR4302547.6078743/1‑94 (MQ=60)
                                                                                   ATCGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATG            <  SRR4302547.778612/94‑1 (MQ=60)
                                                                                     CGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCTAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCC          >  SRR4302547.2493040/1‑94 (MQ=60)
                                                                                     CGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCC          <  SRR4302547.2325288/94‑1 (MQ=60)
                                                                                     CGATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGAGGACGGCTAAAGATGTCACTGATGCC          >  SRR4302547.4597372/1‑94 (MQ=60)
                                                                                      GATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCA         >  SRR4302547.962038/1‑94 (MQ=60)
                                                                                      GATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCA         >  SRR4302547.4339186/1‑94 (MQ=60)
                                                                                       ATGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCCACAGTTTCCAGATGACGGGTAAAGATGTCACTGAactcct        >  SRR4302547.547330/1‑88 (MQ=60)
                                                                                        TGCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATT       <  SRR4302547.1949197/94‑1 (MQ=60)
                                                                                         GCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTG      <  SRR4302547.4759201/94‑1 (MQ=60)
                                                                                         GCCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTG      >  SRR4302547.3249550/1‑94 (MQ=60)
                                                                                          CCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGA     <  SRR4302547.3250027/94‑1 (MQ=60)
                                                                                          CCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGA     >  SRR4302547.2572367/1‑94 (MQ=60)
                                                                                          CCTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGA     >  SRR4302547.1876317/1‑94 (MQ=60)
                                                                                           CTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAG    <  SRR4302547.2019348/94‑1 (MQ=60)
                                                                                           CTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAG    <  SRR4302547.2715336/94‑1 (MQ=60)
                                                                                           CTGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAG    >  SRR4302547.831980/1‑94 (MQ=60)
                                                                                            TGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGT   <  SRR4302547.2292890/94‑1 (MQ=60)
                                                                                            TGTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGT   <  SRR4302547.2690694/94‑1 (MQ=60)
                                                                                             GTGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCATTGATGCCATTGAGTC  <  SRR4302547.4139191/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTC  >  NC_000913/2586054‑2586240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑