Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,610,050 T→C L44P (CTG→CCG)  b2491 → HyfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,610,0500TC83.3% 499.6 / 98.8 217L44P (CTG→CCG) b2491HyfR
Reads supporting (aligned to +/- strand):  ref base T (24/12);  new base C (80/99);  total (105/112)
Fisher's exact test for biased strand distribution p-value = 1.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-02

Only 100 of 250 total aligned reads displayed.

GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGA  >  NC_000913/2609960‑2610142
                                                                                          |                                                                                            
gAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCcgccgc                                                                                            <  1:5136481/93‑1 (MQ=255)
gAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCcgccgc                                                                                            >  2:1657425/1‑93 (MQ=255)
gAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgc                                                                                            >  2:4644645/1‑93 (MQ=255)
gAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgc                                                                                            <  1:2519289/93‑1 (MQ=255)
gAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgc                                                                                            >  1:298107/1‑93 (MQ=255)
 aaTAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCgccgcc                                                                                           >  1:2580770/1‑93 (MQ=255)
  aTAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCccgccgccg                                                                                          <  1:6428551/93‑1 (MQ=255)
   tAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCcgccgccgc                                                                                         >  1:1373864/1‑93 (MQ=255)
       aaTTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCgccgccgccgcc                                                                                     >  2:2126430/1‑93 (MQ=255)
        aTTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGccgccgccgccg                                                                                    <  1:6152081/93‑1 (MQ=255)
          tGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGtt                                                                                  <  1:4900824/93‑1 (MQ=255)
            aaGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCa                                                                                <  2:3005625/93‑1 (MQ=255)
            aaGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCa                                                                                >  1:1526579/1‑93 (MQ=255)
            aaGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCa                                                                                <  2:1152286/93‑1 (MQ=255)
              gCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGc                                                                              <  2:5898092/93‑1 (MQ=255)
               cGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGcc                                                                             <  2:3038733/93‑1 (MQ=255)
               cGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGcc                                                                             >  2:6327/1‑93 (MQ=255)
               cGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGcc                                                                             <  1:2312398/93‑1 (MQ=255)
                ggTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGccc                                                                            >  1:5262415/1‑93 (MQ=255)
                ggTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCAGCAGCCGCCGCCGTTCAGccc                                                                            >  2:6187286/1‑93 (MQ=255)
                 gTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCg                                                                           <  1:1746210/93‑1 (MQ=255)
                  tAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTCTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCgg                                                                          <  2:4661729/93‑1 (MQ=255)
                   aaaCGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAATGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGt                                                                         <  1:3430036/93‑1 (MQ=255)
                   aaaCGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGt                                                                         <  2:5261437/93‑1 (MQ=255)
                       ggAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACaa                                                                     >  1:2552395/1‑93 (MQ=255)
                        gAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAAc                                                                    >  2:2664093/1‑93 (MQ=255)
                         aaTGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACt                                                                   >  2:5828674/1‑93 (MQ=255)
                         aaTGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACt                                                                   <  1:5709889/93‑1 (MQ=255)
                         aaTGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACt                                                                   <  1:906069/93‑1 (MQ=255)
                          aTGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACtt                                                                  >  2:151733/1‑93 (MQ=255)
                          aTGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACtt                                                                  >  1:4781700/1‑93 (MQ=255)
                            gCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTAt                                                                >  2:2760050/1‑93 (MQ=255)
                             cTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTAtt                                                               >  2:6548760/1‑93 (MQ=255)
                             cTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTAtt                                                               <  2:735614/93‑1 (MQ=255)
                             cTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTAtt                                                               <  1:1154624/93‑1 (MQ=255)
                                gcgGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGaa                                                            >  1:1214368/1‑93 (MQ=255)
                                 cgGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAAc                                                           <  2:4976817/93‑1 (MQ=255)
                                 cgGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAAc                                                           <  1:1917637/93‑1 (MQ=255)
                                  ggAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACt                                                          <  2:4705562/93‑1 (MQ=255)
                                  ggAGCGGTTAGAACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCTCCGCTGCCGTTCAGCCCGGTACAAATTATTGAACt                                                          >  1:3363528/1‑93 (MQ=255)
                                   gAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTg                                                         <  1:5570587/93‑1 (MQ=255)
                                   gAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTg                                                         >  2:4005100/1‑93 (MQ=255)
                                    aGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGc                                                        <  2:2437173/93‑1 (MQ=255)
                                    aGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGc                                                        <  2:5652880/93‑1 (MQ=255)
                                     gCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCa                                                       <  2:4372675/93‑1 (MQ=255)
                                      cGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCAt                                                      >  1:3708665/1‑93 (MQ=255)
                                       ggTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATg                                                     <  1:176532/93‑1 (MQ=255)
                                         ttAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGtt                                                   >  2:3332251/1‑93 (MQ=255)
                                           tgCACAGAAACACGGCATGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTtct                                                 <  2:813089/92‑1 (MQ=255)
                                           aGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTtct                                                 >  2:4090276/1‑93 (MQ=255)
                                           aGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTtct                                                 <  1:4096681/93‑1 (MQ=255)
                                           aGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTtct                                                 >  2:1234379/1‑93 (MQ=255)
                                            gCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTtctc                                                <  1:3824693/93‑1 (MQ=255)
                                              acaGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCaa                                              <  2:2590358/93‑1 (MQ=255)
                                               caGAAACACGGCAAGGCGTCTTTAGTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCaaa                                             >  1:6406485/1‑93 (MQ=255)
                                                aGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCaaaa                                            <  1:6611088/93‑1 (MQ=255)
                                                 gAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAg                                           >  1:2403366/1‑93 (MQ=255)
                                                 gAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAg                                           >  1:5968277/1‑93 (MQ=255)
                                                 gAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAg                                           >  2:292970/1‑93 (MQ=255)
                                                      acGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACt                                      >  2:4833885/1‑93 (MQ=255)
                                                       cGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACtt                                     <  2:3939777/93‑1 (MQ=255)
                                                       cGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACtt                                     <  2:375620/93‑1 (MQ=255)
                                                          gaaGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTa                                  <  1:4511113/92‑1 (MQ=255)
                                                          cAAGGCGTCTTTATTACGCGCCTTCAGCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTa                                  <  1:4971825/93‑1 (MQ=255)
                                                            aGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTAtt                                >  2:5285085/1‑93 (MQ=255)
                                                            aGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTAtt                                <  2:5964933/93‑1 (MQ=255)
                                                             ggCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTa                               <  2:5423254/93‑1 (MQ=255)
                                                              gCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTAc                              <  1:5462387/93‑1 (MQ=255)
                                                              gCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTAc                              <  1:5362933/93‑1 (MQ=255)
                                                              gCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTAc                              <  2:18745/93‑1 (MQ=255)
                                                                 tCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGc                           <  1:4197287/93‑1 (MQ=255)
                                                                  cTTTATTACGCTCCTTCATCCCGCCGCCGCTGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCt                          <  2:1731895/93‑1 (MQ=255)
                                                                  cTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCt                          <  1:3912911/93‑1 (MQ=255)
                                                                  cTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCt                          >  2:6359519/1‑93 (MQ=255)
                                                                   tttATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTa                         >  2:2707422/1‑93 (MQ=255)
                                                                    ttattaCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAGCTTCTATTACCGCTAc                        <  2:5794370/93‑1 (MQ=255)
                                                                    ttattaCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTAc                        >  2:1010935/1‑93 (MQ=255)
                                                                     tattaCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTAcc                       <  1:3211613/93‑1 (MQ=255)
                                                                     tattaCGCCCCTTCATCCCGACGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTAcc                       <  2:2115144/93‑1 (MQ=255)
                                                                       ttaCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCat                     <  2:3944721/93‑1 (MQ=255)
                                                                         aCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCatga                   <  2:789302/93‑1 (MQ=255)
                                                                          cgcgCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCatgat                  <  1:3503399/93‑1 (MQ=255)
                                                                           gcgcCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCatgatg                 >  1:2651827/1‑93 (MQ=255)
                                                                              ccTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTTAAAAGCAACTTCTATTACCGCTACCatgatgatg              >  2:2183093/1‑93 (MQ=255)
                                                                               cTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATgg             >  1:1390067/1‑93 (MQ=255)
                                                                                ttCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGc            >  2:5013460/1‑93 (MQ=255)
                                                                                ttCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGc            >  2:6271483/1‑93 (MQ=255)
                                                                                ttCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGc            <  1:6630737/93‑1 (MQ=255)
                                                                                 tCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCa           >  2:1984475/1‑93 (MQ=255)
                                                                                  cATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAg          >  1:1487452/1‑93 (MQ=255)
                                                                                    tCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCg        >  1:6379337/1‑93 (MQ=255)
                                                                                     cccGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGa       >  2:4561525/1‑93 (MQ=255)
                                                                                     cccGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGa       >  2:3619743/1‑93 (MQ=255)
                                                                                     cccGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGa       <  1:5662392/93‑1 (MQ=255)
                                                                                      ccgccgccgccgCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAt      <  1:3541813/93‑1 (MQ=255)
                                                                                      ccgccgccgccgCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAt      <  1:4907052/93‑1 (MQ=255)
                                                                                      ccgccgccgccgCCGTTCAGCCCGGTACAACTTATTGAACTGCATGGTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAt      >  1:6282286/1‑93 (MQ=255)
                                                                                       cgccgccgccgcCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAtg     <  1:2812245/93‑1 (MQ=255)
                                                                                       cGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAtg     >  1:4015198/1‑93 (MQ=255)
                                                                                         cTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAtgtg   >  1:925198/1‑93 (MQ=255)
                                                                                          |                                                                                            
GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGA  >  NC_000913/2609960‑2610142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTG  >  NC_000913/2609957‑2610141
                                                                                             |                                                                                           
AAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCC                                                                                             <  SRR4302547.3824715/94‑1 (MQ=60)
AAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCC                                                                                             <  SRR4302547.2462845/94‑1 (MQ=60)
 AGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCG                                                                                            >  SRR4302547.3969024/1‑94 (MQ=60)
  GGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGC                                                                                           >  SRR4302547.303654/1‑94 (MQ=60)
  GGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGC                                                                                           <  SRR4302547.497931/94‑1 (MQ=60)
  AGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGC                                                                                           <  SRR4302547.2345268/94‑1 (MQ=60)
  AGAATAACAATTAAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCCCCTTCATCCCGCCGC                                                                                           <  SRR4302547.3013974/94‑1 (MQ=60)
   GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCC                                                                                          <  SRR4302547.2570208/94‑1 (MQ=60)
   GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCC                                                                                          <  SRR4302547.5242305/94‑1 (MQ=60)
   GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCC                                                                                          >  SRR4302547.5137291/1‑94 (MQ=60)
   GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCC                                                                                          >  SRR4302547.2632852/1‑94 (MQ=60)
   GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCC                                                                                          >  SRR4302547.4228421/1‑94 (MQ=60)
   GAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCC                                                                                          >  SRR4302547.3781956/1‑94 (MQ=60)
     ATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGC                                                                                        <  SRR4302547.6562566/94‑1 (MQ=60)
     ATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGC                                                                                        >  SRR4302547.1401413/1‑94 (MQ=60)
     ATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGC                                                                                        <  SRR4302547.1875827/94‑1 (MQ=60)
     ATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACACGCCTTCATCACGCCGCCGC                                                                                        <  SRR4302547.6631581/94‑1 (MQ=60)
      TAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGAGGTTAGCACAGAAACACGGCAAGGCGTCGTTATTACGCGCCTTCATCCCGCCGCCGCC                                                                                       <  SRR4302547.2928437/94‑1 (MQ=60)
       AACAATTGAAGCGGTAAACGGAATGCTAGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCG                                                                                      >  SRR4302547.1790751/1‑94 (MQ=60)
          AATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCG                                                                                   >  SRR4302547.3839801/1‑94 (MQ=60)
           ATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGT                                                                                  >  SRR4302547.3486019/1‑94 (MQ=60)
           ATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGT                                                                                  >  SRR4302547.5329974/1‑94 (MQ=60)
           ATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGT                                                                                  <  SRR4302547.6279729/94‑1 (MQ=60)
             TGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTC                                                                                <  SRR4302547.3258080/94‑1 (MQ=60)
             TGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTC                                                                                <  SRR4302547.5002303/94‑1 (MQ=60)
              GAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCA                                                                               >  SRR4302547.121560/1‑94 (MQ=60)
              GAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCA                                                                               <  SRR4302547.2695953/94‑1 (MQ=60)
              GAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCA                                                                               >  SRR4302547.1556960/1‑94 (MQ=60)
               AAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAG                                                                              >  SRR4302547.4392584/1‑94 (MQ=60)
                  CGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCC                                                                           <  SRR4302547.2359282/94‑1 (MQ=60)
                  CGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCC                                                                           >  SRR4302547.5370778/1‑94 (MQ=60)
                   GGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCG                                                                          <  SRR4302547.487762/94‑1 (MQ=60)
                    GTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGG                                                                         <  SRR4302547.1780647/94‑1 (MQ=60)
                     TAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGT                                                                        >  SRR4302547.5801676/1‑94 (MQ=60)
                      AAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAATGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTA                                                                       <  SRR4302547.3499915/94‑1 (MQ=60)
                      AAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTA                                                                       <  SRR4302547.2451447/94‑1 (MQ=60)
                      AAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTA                                                                       <  SRR4302547.3369380/94‑1 (MQ=60)
                      AAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTA                                                                       <  SRR4302547.6541767/94‑1 (MQ=60)
                        ACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACA                                                                     >  SRR4302547.491059/1‑94 (MQ=60)
                         CGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAA                                                                    >  SRR4302547.2603940/1‑94 (MQ=60)
                          GGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCTCGGTACAAC                                                                   >  SRR4302547.2571260/1‑94 (MQ=60)
                          GGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAAC                                                                   >  SRR4302547.6050528/1‑94 (MQ=60)
                           GAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGTCGCCGTTCAGCCCGGTACAACT                                                                  <  SRR4302547.4312847/94‑1 (MQ=60)
                           GAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACT                                                                  >  SRR4302547.2603311/1‑94 (MQ=60)
                           GAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACT                                                                  >  SRR4302547.3504491/1‑94 (MQ=60)
                            AATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTT                                                                 <  SRR4302547.923530/94‑1 (MQ=60)
                            AATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTT                                                                 >  SRR4302547.4880736/1‑94 (MQ=60)
                            AATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTT                                                                 <  SRR4302547.5827823/94‑1 (MQ=60)
                             ATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTA                                                                <  SRR4302547.2799291/94‑1 (MQ=60)
                             ATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTA                                                                >  SRR4302547.1434226/1‑94 (MQ=60)
                              TGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTAT                                                               <  SRR4302547.1870537/94‑1 (MQ=60)
                               GCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATT                                                              <  SRR4302547.6219377/94‑1 (MQ=60)
                                CTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTG                                                             <  SRR4302547.1177987/94‑1 (MQ=60)
                                  CGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAA                                                           >  SRR4302547.1238866/1‑94 (MQ=60)
                                   GCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGACGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAAC                                                          <  SRR4302547.6763081/94‑1 (MQ=60)
                                    CGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACT                                                         <  SRR4302547.1955614/94‑1 (MQ=60)
                                    CGGAGCGGTTAGAACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCTCCGCTGCCGTTCAGCCCGGTACAAATTATTGAACT                                                         >  SRR4302547.3431814/1‑94 (MQ=60)
                                     GGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTG                                                        >  SRR4302547.2065004/1‑94 (MQ=60)
                                     GGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTG                                                        <  SRR4302547.6534002/94‑1 (MQ=60)
                                      GAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGC                                                       <  SRR4302547.5685453/94‑1 (MQ=60)
                                        ggggtaagcccagaagcacgccagggcgccttaattcCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCAT                                                     <  SRR4302547.1772474/57‑1 (MQ=60)
                                        GCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCAT                                                     <  SRR4302547.6333036/94‑1 (MQ=60)
                                        GCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCAT                                                     >  SRR4302547.3784370/1‑94 (MQ=60)
                                        GCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCAT                                                     <  SRR4302547.4050593/94‑1 (MQ=60)
                                          GGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGGACTGCATGT                                                   >  SRR4302547.5025151/1‑94 (MQ=60)
                                          GGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGT                                                   <  SRR4302547.5009362/94‑1 (MQ=60)
                                          GGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGT                                                   <  SRR4302547.179792/94‑1 (MQ=60)
                                           GTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACCACTTATTGAACTGCATGTT                                                  >  SRR4302547.1362443/1‑94 (MQ=60)
                                           GTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTT                                                  <  SRR4302547.957215/94‑1 (MQ=60)
                                           GTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTT                                                  <  SRR4302547.6688866/94‑1 (MQ=60)
                                           GTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTT                                                  <  SRR4302547.1420843/94‑1 (MQ=60)
                                           GTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTT                                                  <  SRR4302547.3589779/94‑1 (MQ=60)
                                           GTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTT                                                  <  SRR4302547.3554678/94‑1 (MQ=60)
                                             TAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCT                                                <  SRR4302547.5933719/94‑1 (MQ=60)
                                             TAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCT                                                <  SRR4302547.5492687/94‑1 (MQ=60)
                                              AGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTC                                               <  SRR4302547.4179990/94‑1 (MQ=60)
                                               GCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCA                                              >  SRR4302547.3013770/1‑94 (MQ=60)
                                               GCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCA                                              <  SRR4302547.3304674/94‑1 (MQ=60)
                                               GCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCA                                              <  SRR4302547.3902607/94‑1 (MQ=60)
                                               GCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCACCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCA                                              <  SRR4302547.3942479/94‑1 (MQ=60)
                                                 ACAGAAACACGGCAAGGCGTCTTTAGTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAA                                            >  SRR4302547.6540093/1‑94 (MQ=60)
                                                   AGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAG                                          >  SRR4302547.6092067/1‑94 (MQ=60)
                                                   AGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAG                                          <  SRR4302547.6748692/94‑1 (MQ=60)
                                                   AGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAG                                          <  SRR4302547.1607533/94‑1 (MQ=60)
                                                   AGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAG                                          >  SRR4302547.2452036/1‑94 (MQ=60)
                                                     AACCACGGCAAGGCTTCTTTATGACCCTCCTTCACCCC‑‑‑GCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACT                                     <  SRR4302547.2623539/94‑1 (MQ=60)
                                                     AAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCA                                        <  SRR4302547.749355/94‑1 (MQ=60)
                                                       ACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAAC                                      <  SRR4302547.6434973/94‑1 (MQ=60)
                                                        CACGGCAAGGCGTCTTTATTGCGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAGCTTATTGAACTGCATGTTCTCAAAAGCAACT                                     <  SRR4302547.749669/94‑1 (MQ=60)
                                                        CACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACT                                     <  SRR4302547.4714574/94‑1 (MQ=60)
                                                        CACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCC‑TTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTT                                    <  SRR4302547.4108313/94‑1 (MQ=60)
                                                         ACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTT                                    >  SRR4302547.5721158/1‑94 (MQ=60)
                                                         ACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTT                                    >  SRR4302547.2379183/1‑94 (MQ=60)
                                                         ACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTT                                    <  SRR4302547.5006079/94‑1 (MQ=60)
                                                          CGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTC                                   >  SRR4302547.6367296/1‑94 (MQ=60)
                                                            GCAAGGCTTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTA                                 <  SRR4302547.5632450/94‑1 (MQ=60)
                                                             GAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTAT                                <  SRR4302547.4604316/94‑1 (MQ=60)
                                                             CAAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTAT                                <  SRR4302547.1540613/94‑1 (MQ=60)
                                                             CAAGGCGTCTTTATTACGCGCCTTCAGCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTAT                                <  SRR4302547.5074688/94‑1 (MQ=60)
                                                              AAGGCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCCACTTCTATT                               >  SRR4302547.550315/1‑94 (MQ=60)
                                                                 GCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACC                            <  SRR4302547.5404321/94‑1 (MQ=60)
                                                                 GCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACC                            <  SRR4302547.5473555/94‑1 (MQ=60)
                                                                 GCGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACC                            <  SRR4302547.5575226/94‑1 (MQ=60)
                                                                  CGTCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCG                           >  SRR4302547.266414/1‑94 (MQ=60)
                                                                   GTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGC                          >  SRR4302547.1580530/1‑94 (MQ=60)
                                                                    TCTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCT                         <  SRR4302547.4282883/94‑1 (MQ=60)
                                                                     CTTTATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTA                        <  SRR4302547.3992472/94‑1 (MQ=60)
                                                                       TTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACC                      >  SRR4302547.5365643/1‑94 (MQ=60)
                                                                       TTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACC                      >  SRR4302547.1342116/1‑94 (MQ=60)
                                                                        TATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCA                     >  SRR4302547.6537923/1‑94 (MQ=60)
                                                                        TATTACGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCA                     <  SRR4302547.3275680/94‑1 (MQ=60)
                                                                         tttgCGCGCTTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGATCTGCATGTTCTCAAAGGCAACTTCTATTACCGCTACCAT                    <  SRR4302547.4103131/90‑1 (MQ=60)
                                                                             CGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATG                >  SRR4302547.2705144/1‑94 (MQ=60)
                                                                             CGCGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATG                <  SRR4302547.3574896/94‑1 (MQ=60)
                                                                               CGCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGAT              <  SRR4302547.1413006/94‑1 (MQ=60)
                                                                                GCCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATG             >  SRR4302547.2491278/1‑94 (MQ=60)
                                                                                 CCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGG            >  SRR4302547.1417889/1‑94 (MQ=60)
                                                                                 CCTTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTAACGCTACCATGATGATGG            <  SRR4302547.941480/94‑1 (MQ=60)
                                                                                   TTCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCA          <  SRR4302547.6768767/94‑1 (MQ=60)
                                                                                    TCATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAG         >  SRR4302547.1517143/1‑94 (MQ=60)
                                                                                      CTCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCG       <  SRR4302547.3625130/94‑1 (MQ=60)
                                                                                      ATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCG       <  SRR4302547.5760962/94‑1 (MQ=60)
                                                                                      ATCCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCG       >  SRR4302547.6512464/1‑94 (MQ=60)
                                                                                        CCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAT     <  SRR4302547.5779285/94‑1 (MQ=60)
                                                                                        CCCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGGTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGAT     >  SRR4302547.6412808/1‑94 (MQ=60)
                                                                                         CCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATG    >  SRR4302547.4096798/1‑94 (MQ=60)
                                                                                         CCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATG    <  SRR4302547.3614112/94‑1 (MQ=60)
                                                                                         CCGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATG    <  SRR4302547.5008668/94‑1 (MQ=60)
                                                                                          CGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGT   <  SRR4302547.2868508/94‑1 (MQ=60)
                                                                                          CGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGT   <  SRR4302547.3916176/94‑1 (MQ=60)
                                                                                          CGCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGT   >  SRR4302547.2876802/1‑94 (MQ=60)
                                                                                           GCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTG  >  SRR4302547.943067/1‑94 (MQ=60)
                                                                                           GCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTG  <  SRR4302547.4305122/94‑1 (MQ=60)
                                                                                           GCCGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTG  <  SRR4302547.3339369/94‑1 (MQ=60)
                                                                                             |                                                                                           
AAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTG  >  NC_000913/2609957‑2610141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑