Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,635,451 G→A intergenic (‑36/+43) b2511 ← / ← b2512 putative GTP‑binding factor/putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,635,4510GA88.1% 797.4 / 72.1 280intergenic (‑36/+43)b2511/b2512putative GTP‑binding factor/putative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (20/13);  new base A (129/116);  total (149/131)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 292 total aligned reads displayed.

GACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTT  >  NC_000913/2635362‑2635543
                                                                                         |                                                                                            
gACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGAccc                                                                                           <  1:387285/93‑1 (MQ=255)
 aCCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGAcccc                                                                                          <  2:831885/93‑1 (MQ=255)
  ccACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCt                                                                                         <  1:5923614/93‑1 (MQ=255)
     caGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTc                                                                                      >  2:890189/1‑93 (MQ=255)
     caGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTc                                                                                      <  1:907329/93‑1 (MQ=255)
      aGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTcc                                                                                     <  1:1099048/93‑1 (MQ=255)
      aGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTcc                                                                                     >  2:4259763/1‑93 (MQ=255)
      aGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTcc                                                                                     >  2:3807611/1‑93 (MQ=255)
            cATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGc                                                                               >  2:1432173/1‑93 (MQ=255)
            cATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGc                                                                               <  2:1234026/93‑1 (MQ=255)
             aTGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAACACAGGAAAACGACCCCTGTCCAGGAGcc                                                                              >  1:3286692/1‑93 (MQ=255)
             aTGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGcc                                                                              <  1:1537138/93‑1 (MQ=255)
              tGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCg                                                                             <  1:3462530/93‑1 (MQ=255)
                tttAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtt                                                                           >  2:1523578/1‑93 (MQ=255)
                 ttAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGttt                                                                          <  1:3755315/93‑1 (MQ=255)
                  tAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtttt                                                                         <  1:2547414/93‑1 (MQ=255)
                  tAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGtttt                                                                         >  2:723438/1‑93 (MQ=255)
                   aaaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTc                                                                        >  1:3063833/1‑93 (MQ=255)
                    aaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCa                                                                       >  2:4726509/1‑93 (MQ=255)
                      gCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                     >  1:4223082/1‑93 (MQ=255)
                       ccTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAg                                                                    >  1:3111958/1‑93 (MQ=255)
                        cTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGt                                                                   >  2:5024022/1‑93 (MQ=255)
                        cTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGt                                                                   >  1:5359092/1‑93 (MQ=255)
                         tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTg                                                                  >  1:5856287/1‑93 (MQ=255)
                         tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTg                                                                  <  2:2747327/93‑1 (MQ=255)
                          cATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGa                                                                 >  1:778046/1‑93 (MQ=255)
                          cATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGa                                                                 >  2:5353319/1‑93 (MQ=255)
                           aTTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGaa                                                                <  1:571255/93‑1 (MQ=255)
                           aTTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGaa                                                                <  1:2155400/93‑1 (MQ=255)
                           aTTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGaa                                                                <  1:719364/93‑1 (MQ=255)
                            tttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAc                                                               >  2:5804084/1‑93 (MQ=255)
                            tttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAc                                                               >  2:6457183/1‑93 (MQ=255)
                            tttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAc                                                               <  2:70634/93‑1 (MQ=255)
                             ttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACg                                                              <  1:4134527/93‑1 (MQ=255)
                             ttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACg                                                              >  2:773141/1‑93 (MQ=255)
                               ttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAc                                                            <  2:5112350/93‑1 (MQ=255)
                               ttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAc                                                            <  2:3310110/93‑1 (MQ=255)
                                tataAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACa                                                           >  2:4902954/1‑93 (MQ=255)
                                tataAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACa                                                           >  1:5436153/1‑93 (MQ=255)
                                 ataAATCATCAGACAACGCGCGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACag                                                          <  2:4798378/93‑1 (MQ=255)
                                 ataAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACag                                                          <  2:4405335/93‑1 (MQ=255)
                                  taAATCATCAGACAACGCACGCTATATTCGGGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACaga                                                         <  2:1616393/93‑1 (MQ=255)
                                   aaaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACagag                                                        <  1:2375971/93‑1 (MQ=255)
                                        atcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGAt                                                   <  2:1050638/93‑1 (MQ=255)
                                         tcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGAtt                                                  >  2:6552189/1‑93 (MQ=255)
                                         tcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGAtt                                                  <  2:3606956/93‑1 (MQ=255)
                                          caGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTa                                                 >  1:2819216/1‑93 (MQ=255)
                                           aGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTaa                                                <  2:235650/93‑1 (MQ=255)
                                           aGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTaa                                                <  2:2010564/93‑1 (MQ=255)
                                            gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                               >  1:3591535/1‑93 (MQ=255)
                                            gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                               <  1:6019471/93‑1 (MQ=255)
                                            gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                               >  2:5245363/1‑93 (MQ=255)
                                            gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                               >  1:1823610/1‑93 (MQ=255)
                                             aCAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACg                                              >  2:2596389/1‑93 (MQ=255)
                                              cAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgt                                             >  1:3001968/1‑93 (MQ=255)
                                              cAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgt                                             >  1:4316237/1‑93 (MQ=255)
                                                acgcacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtgt                                           >  1:479235/1‑93 (MQ=255)
                                                  gcacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTaa                                         >  2:5693712/1‑93 (MQ=255)
                                                   cacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAAt                                        <  2:3005133/93‑1 (MQ=255)
                                                    acgcTATATTCGCGTCGTTGTTAAAAACAGGAAAGCGACCCCTGCCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                       <  2:4525770/93‑1 (MQ=255)
                                                    acgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                       <  1:2969479/93‑1 (MQ=255)
                                                      gcTATATGCGCGTCGTTGATAAAAATAGTACAACGACCCCTGCCCAGGACCCGTTTTCAAAGTGAACGACAGAGTCGATTAACGTGTAATaga                                     <  2:3852229/93‑1 (MQ=255)
                                                       cTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATagag                                    <  1:1129186/93‑1 (MQ=255)
                                                        tataTTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGt                                   >  1:1283193/1‑93 (MQ=255)
                                                        tataTTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATagaga                                   >  1:1466719/1‑92 (MQ=255)
                                                        tataTTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGt                                   <  1:1770037/93‑1 (MQ=255)
                                                        tataTTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGt                                   <  2:403099/93‑1 (MQ=255)
                                                          tatTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTac                                 >  2:2664703/1‑93 (MQ=255)
                                                          tatTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTac                                 <  2:547369/93‑1 (MQ=255)
                                                          tatTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTaa                                 >  1:3629544/1‑92 (MQ=255)
                                                           atTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTaca                                >  2:3885405/1‑93 (MQ=255)
                                                           atTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTaca                                >  2:2205617/1‑93 (MQ=255)
                                                           atTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTaca                                <  2:5109294/93‑1 (MQ=255)
                                                            ttCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                               >  2:3485704/1‑93 (MQ=255)
                                                               gcgTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGt                            <  1:4910525/93‑1 (MQ=255)
                                                                 gtcgtTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTc                          >  2:4161683/1‑93 (MQ=255)
                                                                  tcgtTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTcc                         <  1:4273757/93‑1 (MQ=255)
                                                                  tcgtTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTcc                         >  1:5658762/1‑93 (MQ=255)
                                                                   cgtTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCg                        >  1:4503815/1‑93 (MQ=255)
                                                                    tttgttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                       <  2:1278415/92‑1 (MQ=255)
                                                                    gttgttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                       >  2:1870381/1‑93 (MQ=255)
                                                                     ttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTc                      <  2:5692774/93‑1 (MQ=255)
                                                                     ttgttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTc                      <  2:4600479/93‑1 (MQ=255)
                                                                     ttgttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTc                      >  1:3256929/1‑93 (MQ=255)
                                                                      tgttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCt                     >  1:6542897/1‑93 (MQ=255)
                                                                       gttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCtt                    <  1:5085948/93‑1 (MQ=255)
                                                                       gttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCtt                    <  2:557124/93‑1 (MQ=255)
                                                                       gttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCtt                    >  2:2097461/1‑93 (MQ=255)
                                                                        ttAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCttt                   <  1:1006653/93‑1 (MQ=255)
                                                                         tAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCtttt                  >  1:6135107/1‑93 (MQ=255)
                                                                          aaaaaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTg                 <  1:3929221/93‑1 (MQ=255)
                                                                          aaaaaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTg                 <  1:5815890/93‑1 (MQ=255)
                                                                           aaaaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGc                >  2:5729329/1‑93 (MQ=255)
                                                                            aaaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGcc               >  1:5179065/1‑93 (MQ=255)
                                                                             aaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt              <  1:3479608/93‑1 (MQ=255)
                                                                             aaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt              >  1:4623031/1‑93 (MQ=255)
                                                                             aaCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt              >  1:641980/1‑93 (MQ=255)
                                                                                   aaaaCGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATca        <  2:1661668/93‑1 (MQ=255)
                                                                                    aaaCGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATcag       <  2:6409331/93‑1 (MQ=255)
                                                                                        ggCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGt   <  2:62626/93‑1 (MQ=255)
                                                                                         |                                                                                            
GACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTT  >  NC_000913/2635362‑2635543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTT  >  NC_000913/2635359‑2635544
                                                                                            |                                                                                             
CGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGAC                                                                                              <  SRR4302547.5453463/94‑1 (MQ=60)
  CGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCC                                                                                            >  SRR4302547.1161528/1‑94 (MQ=60)
   GACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCC                                                                                           <  SRR4302547.394467/94‑1 (MQ=60)
     CCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTG                                                                                         <  SRR4302547.6046389/94‑1 (MQ=60)
       ACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTC                                                                                       >  SRR4302547.978468/1‑94 (MQ=60)
       ACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTC                                                                                       <  SRR4302547.4207219/94‑1 (MQ=60)
       ACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTC                                                                                       <  SRR4302547.3983585/94‑1 (MQ=60)
       ACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTC                                                                                       <  SRR4302547.4333908/94‑1 (MQ=60)
        CAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCC                                                                                      >  SRR4302547.2437280/1‑94 (MQ=60)
        CAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCC                                                                                      <  SRR4302547.924818/94‑1 (MQ=60)
        CAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCC                                                                                      >  SRR4302547.3685078/1‑94 (MQ=60)
         AGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCA                                                                                     >  SRR4302547.1147358/1‑94 (MQ=60)
         AGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCA                                                                                     <  SRR4302547.1121121/94‑1 (MQ=60)
         AGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCA                                                                                     >  SRR4302547.3594477/1‑94 (MQ=60)
         AGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAAAAGCCCCTGTCCA                                                                                     >  SRR4302547.4250282/1‑94 (MQ=60)
           GTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGA                                                                                   >  SRR4302547.3691514/1‑94 (MQ=60)
            TACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGA                                                                                  >  SRR4302547.3304727/1‑94 (MQ=60)
            TACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGA                                                                                  >  SRR4302547.5335975/1‑94 (MQ=60)
             ACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAG                                                                                 <  SRR4302547.23731/94‑1 (MQ=60)
             ACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAG                                                                                 >  SRR4302547.4694719/1‑94 (MQ=60)
             ACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAG                                                                                 >  SRR4302547.5171956/1‑94 (MQ=60)
             ACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAG                                                                                 <  SRR4302547.4875239/94‑1 (MQ=60)
              CCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGC                                                                                <  SRR4302547.2179057/94‑1 (MQ=60)
              CCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGC                                                                                <  SRR4302547.1741676/94‑1 (MQ=60)
              CCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGC                                                                                >  SRR4302547.2468818/1‑94 (MQ=60)
               CATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAACACAGGAAAACGACCCCTGTCCAGGAGCC                                                                               >  SRR4302547.3352621/1‑94 (MQ=60)
               CATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCC                                                                               <  SRR4302547.1350072/94‑1 (MQ=60)
               CATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCC                                                                               <  SRR4302547.616181/94‑1 (MQ=60)
                ATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCG                                                                              <  SRR4302547.1567738/94‑1 (MQ=60)
                 TGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGT                                                                             <  SRR4302547.3533134/94‑1 (MQ=60)
                 TGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGT                                                                             <  SRR4302547.6142093/94‑1 (MQ=60)
                  GTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTT                                                                            <  SRR4302547.1552639/94‑1 (MQ=60)
                  GTTTAAAGCCTCATTTGTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGCCCCCTGTCCAGGAGCCGTT                                                                            <  SRR4302547.5650774/94‑1 (MQ=60)
                    TTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTT                                                                          <  SRR4302547.3534321/94‑1 (MQ=60)
                    TTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTT                                                                          >  SRR4302547.378683/1‑94 (MQ=60)
                    TTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTT                                                                          <  SRR4302547.3831938/94‑1 (MQ=60)
                     TAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTC                                                                         <  SRR4302547.2598856/94‑1 (MQ=60)
                     TAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTC                                                                         >  SRR4302547.3124758/1‑94 (MQ=60)
                      AAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCA                                                                        >  SRR4302547.4851853/1‑94 (MQ=60)
                        AGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAA                                                                      >  SRR4302547.4309337/1‑94 (MQ=60)
                        AGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAA                                                                      <  SRR4302547.5841634/94‑1 (MQ=60)
                        AGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAA                                                                      >  SRR4302547.4130862/1‑94 (MQ=60)
                         GCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAG                                                                     >  SRR4302547.3173628/1‑94 (MQ=60)
                         GCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAG                                                                     >  SRR4302547.6120884/1‑94 (MQ=60)
                          CCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGT                                                                    >  SRR4302547.5469633/1‑94 (MQ=60)
                           CTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTG                                                                   >  SRR4302547.314147/1‑94 (MQ=60)
                           CTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTG                                                                   >  SRR4302547.3881982/1‑94 (MQ=60)
                           CTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTG                                                                   >  SRR4302547.5977467/1‑94 (MQ=60)
                           CTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTG                                                                   >  SRR4302547.1317577/1‑94 (MQ=60)
                           CTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTG                                                                   >  SRR4302547.5943864/1‑94 (MQ=60)
                            TCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGA                                                                  >  SRR4302547.792757/1‑94 (MQ=60)
                             CATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAA                                                                 >  SRR4302547.3816241/1‑94 (MQ=60)
                              ATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAC                                                                >  SRR4302547.5974965/1‑94 (MQ=60)
                              ATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAC                                                                <  SRR4302547.732727/94‑1 (MQ=60)
                              ATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAC                                                                <  SRR4302547.2198447/94‑1 (MQ=60)
                              ATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAAC                                                                <  SRR4302547.581523/94‑1 (MQ=60)
                               TTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAATCGACCCCTGTCCAGGAACCGTTTTCAAAGTGAACG                                                               <  SRR4302547.756864/94‑1 (MQ=60)
                               TTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACG                                                               >  SRR4302547.3347321/1‑94 (MQ=60)
                                TTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAGCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGA                                                              <  SRR4302547.266243/94‑1 (MQ=60)
                                TTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGA                                                              <  SRR4302547.4218650/94‑1 (MQ=60)
                                 TTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAC                                                             >  SRR4302547.6747759/1‑94 (MQ=60)
                                  TTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACA                                                            >  SRR4302547.5548509/1‑94 (MQ=60)
                                  TTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACA                                                            <  SRR4302547.2248489/94‑1 (MQ=60)
                                   TATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAG                                                           <  SRR4302547.1146591/94‑1 (MQ=60)
                                     TACATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAG                                                         >  SRR4302547.3534061/1‑94 (MQ=60)
                                     TAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAG                                                         <  SRR4302547.4357210/94‑1 (MQ=60)
                                      AAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGA                                                        >  SRR4302547.3643161/1‑94 (MQ=60)
                                      AAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGA                                                        <  SRR4302547.2424110/94‑1 (MQ=60)
                                       AATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGAC                                                       <  SRR4302547.3021544/94‑1 (MQ=60)
                                       AATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGAC                                                       <  SRR4302547.4540373/94‑1 (MQ=60)
                                       AATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGAC                                                       >  SRR4302547.1182712/1‑94 (MQ=60)
                                        ATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACG                                                      <  SRR4302547.1550514/94‑1 (MQ=60)
                                        ATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACG                                                      <  SRR4302547.3217201/94‑1 (MQ=60)
                                          CATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGAT                                                    <  SRR4302547.4935055/94‑1 (MQ=60)
                                          CATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGAT                                                    >  SRR4302547.3164569/1‑94 (MQ=60)
                                            TCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTA                                                  <  SRR4302547.3885221/94‑1 (MQ=60)
                                            TCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTA                                                  >  SRR4302547.2875613/1‑94 (MQ=60)
                                             CAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAA                                                 >  SRR4302547.6315223/1‑94 (MQ=60)
                                              AGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAC                                                >  SRR4302547.1859659/1‑94 (MQ=60)
                                              AGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAC                                                >  SRR4302547.5235968/1‑94 (MQ=60)
                                              AGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAC                                                >  SRR4302547.3664795/1‑94 (MQ=60)
                                              AGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAC                                                >  SRR4302547.824275/1‑94 (MQ=60)
                                               GACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACG                                               <  SRR4302547.6144399/94‑1 (MQ=60)
                                                ACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGT                                              >  SRR4302547.3061664/1‑94 (MQ=60)
                                                ACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGT                                              >  SRR4302547.3061663/1‑94 (MQ=60)
                                                ACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGT                                              >  SRR4302547.4405065/1‑94 (MQ=60)
                                                ACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGT                                              >  SRR4302547.66133/1‑94 (MQ=60)
                                                ACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGT                                              >  SRR4302547.5533391/1‑94 (MQ=60)
                                                  AACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGT                                            >  SRR4302547.488036/1‑94 (MQ=60)
                                                   ACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTA                                           <  SRR4302547.5248604/94‑1 (MQ=60)
                                                      CACCCTATATTCTCGTCGTTGTTAAAAACAGGAACACGTCCCCTGTCCAGGAGTCGTTTTCAAAGTGATCGACAGAGACGATTAACGTGTAATA                                        <  SRR4302547.4915887/94‑1 (MQ=60)
                                                       ACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAG                                       <  SRR4302547.3028584/94‑1 (MQ=60)
                                                         GCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAG                                     >  SRR4302547.3079561/1‑94 (MQ=60)
                                                         GCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAG                                     >  SRR4302547.3113766/1‑94 (MQ=60)
                                                          CTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGT                                    >  SRR4302547.1309049/1‑94 (MQ=60)
                                                          CTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGT                                    <  SRR4302547.294664/94‑1 (MQ=60)
                                                          CTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGT                                    <  SRR4302547.1151976/94‑1 (MQ=60)
                                                          CTATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGA                                    >  SRR4302547.1496022/1‑94 (MQ=60)
                                                           TATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTA                                   >  SRR4302547.3382443/1‑94 (MQ=60)
                                                           TATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTA                                   <  SRR4302547.6561321/94‑1 (MQ=60)
                                                           TATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTA                                   <  SRR4302547.1805133/94‑1 (MQ=60)
                                                           TATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTA                                   >  SRR4302547.2700204/1‑94 (MQ=60)
                                                            TTATTCGCGTCGTTGTTAAAACCAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTAC                                  <  SRR4302547.5924527/94‑1 (MQ=60)
                                                            ATATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTAA                                  >  SRR4302547.3703588/1‑94 (MQ=60)
                                                              ATTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACAC                                >  SRR4302547.4380056/1‑94 (MQ=60)
                                                               TTCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACG                               >  SRR4302547.6004082/1‑94 (MQ=60)
                                                                TCGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGG                              >  SRR4302547.3329562/1‑94 (MQ=60)
                                                                 CGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGATGATTAACGTGTAATAGAGTACACGGT                             >  SRR4302547.820363/1‑94 (MQ=60)
                                                                 CGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGT                             <  SRR4302547.4683518/94‑1 (MQ=60)
                                                                 CGCGTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGT                             <  SRR4302547.5994200/94‑1 (MQ=60)
                                                                  GCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTT                            <  SRR4302547.5012210/94‑1 (MQ=60)
                                                                    GTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCC                          <  SRR4302547.93019/94‑1 (MQ=60)
                                                                    GTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCC                          <  SRR4302547.183107/94‑1 (MQ=60)
                                                                    GTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCC                          >  SRR4302547.5775573/1‑94 (MQ=60)
                                                                    GTCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGGAAACGGTTCC                          >  SRR4302547.5812059/1‑94 (MQ=60)
                                                                     TCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCG                         >  SRR4302547.4596814/1‑94 (MQ=60)
                                                                     TCGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCG                         <  SRR4302547.4361506/94‑1 (MQ=60)
                                                                      CGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGT                        >  SRR4302547.1752247/1‑94 (MQ=60)
                                                                      CGTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGCTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGCTCCGT                        >  SRR4302547.5301160/1‑94 (MQ=60)
                                                                       GTTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTC                       >  SRR4302547.3322009/1‑94 (MQ=60)
                                                                        TTGTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCT                      >  SRR4302547.6679073/1‑94 (MQ=60)
                                                                          GTTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTT                    <  SRR4302547.5190858/94‑1 (MQ=60)
                                                                          GTTAAAAACAGAAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGCCGATTAACGTGTAATAGAGTACACGGTTCCGTCTTT                    <  SRR4302547.695443/94‑1 (MQ=60)
                                                                           TTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTT                   <  SRR4302547.1026306/94‑1 (MQ=60)
                                                                           TTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTT                   <  SRR4302547.6195086/94‑1 (MQ=60)
                                                                           TTAAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTT                   >  SRR4302547.6262381/1‑94 (MQ=60)
                                                                             AAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGC                 <  SRR4302547.5936223/94‑1 (MQ=60)
                                                                             AAAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGC                 <  SRR4302547.4009138/94‑1 (MQ=60)
                                                                              AAAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCC                >  SRR4302547.5285667/1‑94 (MQ=60)
                                                                               AAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCT               >  SRR4302547.653821/1‑94 (MQ=60)
                                                                               AAACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCT               >  SRR4302547.4718762/1‑94 (MQ=60)
                                                                               AAACAGGAAAACGACCCCTGTCCAGGAGCCGGTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGCCTTTTGCCT               >  SRR4302547.4457503/1‑94 (MQ=60)
                                                                                AACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTG              <  SRR4302547.5044294/94‑1 (MQ=60)
                                                                                AACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTG              <  SRR4302547.3550582/94‑1 (MQ=60)
                                                                                 ACAGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAGGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGG             >  SRR4302547.5639224/1‑94 (MQ=60)
                                                                                   AGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGAT           >  SRR4302547.1106898/1‑94 (MQ=60)
                                                                                   AGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGAT           <  SRR4302547.4919711/94‑1 (MQ=60)
                                                                                   AGGAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGAT           >  SRR4302547.4395167/1‑94 (MQ=60)
                                                                                     GAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCA         <  SRR4302547.5543767/94‑1 (MQ=60)
                                                                                     GAAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCA         >  SRR4302547.1866918/1‑94 (MQ=60)
                                                                                      AAAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAG        <  SRR4302547.6274399/94‑1 (MQ=60)
                                                                                       AAACGACCCCTGTCCAGGAGCCGTTTTCAAAGTTAACGACAGAGACGATTAACGTGTAATAGAGTACACGTTTCCGTCTTTTTCCTGGATCAGC       <  SRR4302547.766745/94‑1 (MQ=60)
                                                                                        AACGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCA      >  SRR4302547.3955688/1‑94 (MQ=60)
                                                                                         ACGACCCCTGTCCAGGAGCCGTTTTCAAATTGAACGACAGAGACGATTAACTTGTAATAGAGTAAACGGTTCCGTCTTTTGCCTGGATCAGCAG     >  SRR4302547.980518/1‑94 (MQ=60)
                                                                                          CGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGT    >  SRR4302547.2286977/1‑94 (MQ=60)
                                                                                          CGACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGT    <  SRR4302547.1686719/94‑1 (MQ=60)
                                                                                           GGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTT   >  SRR4302547.3471343/1‑94 (MQ=60)
                                                                                           GGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTT   >  SRR4302547.1848536/1‑94 (MQ=60)
                                                                                            ACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTT  <  SRR4302547.5981062/94‑1 (MQ=60)
                                                                                            ACCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTT  <  SRR4302547.5463706/94‑1 (MQ=60)
                                                                                            |                                                                                             
CGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTT  >  NC_000913/2635359‑2635544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑