Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,637,565 G→A A344V (GCT→GTT)  hisS ← histidine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,5650GA87.5% 594.3 / 58.8 208A344V (GCT→GTT) hisShistidine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (7/19);  new base A (75/107);  total (82/126)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-01

Only 100 of 212 total aligned reads displayed.

GCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAAC  >  NC_000913/2637475‑2637654
                                                                                          |                                                                                         
gCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAAcc                                                                                         >  1:1536635/1‑93 (MQ=255)
  aaaCTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGc                                                                                       >  2:1445373/1‑93 (MQ=255)
   aaCTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCa                                                                                      <  2:5484192/93‑1 (MQ=255)
    aCTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTGATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAg                                                                                     >  2:5666187/1‑93 (MQ=255)
    aCTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAg                                                                                     >  2:2828358/1‑93 (MQ=255)
    aCTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAg                                                                                     >  2:4683550/1‑93 (MQ=255)
         ttCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATtgt                                                                                <  2:4162678/93‑1 (MQ=255)
         ttCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATtgt                                                                                <  2:60885/93‑1 (MQ=255)
          tCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATtgtg                                                                               >  2:2224967/1‑93 (MQ=255)
           cTTAAAGTTGCCGCCGCCGTTGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTgtgt                                                                              <  1:4172407/93‑1 (MQ=255)
            ttAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTa                                                                             >  2:5166364/1‑93 (MQ=255)
            ttAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTa                                                                             <  2:4151252/93‑1 (MQ=255)
             tAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTAt                                                                            >  1:3705843/1‑93 (MQ=255)
              aaaGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATc                                                                           <  2:5833286/93‑1 (MQ=255)
              aaaGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATc                                                                           <  1:1346449/93‑1 (MQ=255)
               aaGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCa                                                                          <  2:163309/93‑1 (MQ=255)
               aaGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCa                                                                          <  2:4861756/93‑1 (MQ=255)
                 gTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGc                                                                        <  2:3960490/93‑1 (MQ=255)
                 gTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGACTGTGTATCAga                                                                        >  1:6333646/1‑92 (MQ=255)
                  ttGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCa                                                                       >  1:2747256/1‑93 (MQ=255)
                   tGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCAc                                                                      <  1:3894151/93‑1 (MQ=255)
                    tccgccgccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCAcc                                                                     <  2:3812075/92‑1 (MQ=255)
                        ccgccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGaa                                                                 <  1:6575497/93‑1 (MQ=255)
                        ccgccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGaa                                                                 <  1:5512444/93‑1 (MQ=255)
                        ccgccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGaa                                                                 <  2:741751/93‑1 (MQ=255)
                         cgccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCACAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAg                                                                <  2:3564861/93‑1 (MQ=255)
                          gccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGc                                                               <  2:1253744/93‑1 (MQ=255)
                           ccgTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGcc                                                              <  2:4970217/93‑1 (MQ=255)
                              tggttggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGccacc                                                           >  2:2760965/1‑93 (MQ=255)
                               ggttggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGccacca                                                          >  2:3752185/1‑93 (MQ=255)
                                gttggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAg                                                         <  1:4895439/93‑1 (MQ=255)
                                gttggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGACGCCACCAg                                                         >  2:6363506/1‑93 (MQ=255)
                                gttggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAg                                                         <  2:2259699/93‑1 (MQ=255)
                                gttggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAg                                                         <  1:962485/93‑1 (MQ=255)
                                  tggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGt                                                       <  1:1070447/93‑1 (MQ=255)
                                  tggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGt                                                       >  1:5651626/1‑93 (MQ=255)
                                   ggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGta                                                      >  1:1016447/1‑93 (MQ=255)
                                   ggtCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGta                                                      <  2:3401116/93‑1 (MQ=255)
                                      catcaATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGtatat                                                   >  2:5777808/1‑93 (MQ=255)
                                      catcaATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGtatat                                                   <  2:2545773/93‑1 (MQ=255)
                                       atcaATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGtatata                                                  >  1:2525175/1‑93 (MQ=255)
                                       atcaATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGtatata                                                  <  1:146261/93‑1 (MQ=255)
                                         caATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATc                                                <  1:696779/93‑1 (MQ=255)
                                          aaTTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCg                                               <  2:2049104/93‑1 (MQ=255)
                                            tttCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGac                                             <  2:3101809/93‑1 (MQ=255)
                                             ttCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGaca                                            <  1:5450595/93‑1 (MQ=255)
                                               cACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGacaac                                          <  2:3308668/93‑1 (MQ=255)
                                               cACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGacaac                                          <  1:191388/93‑1 (MQ=255)
                                               cACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGacaac                                          >  1:5873195/1‑93 (MQ=255)
                                                aCGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGacaaca                                         <  1:3969488/93‑1 (MQ=255)
                                                aCGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGacaaca                                         <  2:2911658/93‑1 (MQ=255)
                                                  gCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAgg                                       <  1:2456548/93‑1 (MQ=255)
                                                   cccGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGa                                      <  2:6155864/93‑1 (MQ=255)
                                                   cccGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGa                                      <  2:2691880/93‑1 (MQ=255)
                                                    ccGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGAt                                     <  1:6191846/93‑1 (MQ=255)
                                                    ccGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGAt                                     <  1:3052488/93‑1 (MQ=255)
                                                    ccGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGAt                                     <  2:4551165/93‑1 (MQ=255)
                                                     cGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATc                                    <  1:437265/93‑1 (MQ=255)
                                                     cGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATc                                    >  1:6438660/1‑93 (MQ=255)
                                                      ggTAATTCATCCCGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCg                                   >  1:4653971/1‑93 (MQ=255)
                                                       gTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCgg                                  >  1:4555290/1‑93 (MQ=255)
                                                         aaTTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCt                                >  2:1928991/1‑93 (MQ=255)
                                                         aaTTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCt                                <  2:3143156/93‑1 (MQ=255)
                                                         aaTTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCt                                >  2:2479728/1‑93 (MQ=255)
                                                           ttCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCCCCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCttt                              <  1:5812813/93‑1 (MQ=255)
                                                            tcatcaCGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTa                             <  1:878798/93‑1 (MQ=255)
                                                               tcaCGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAAt                          <  2:4026357/93‑1 (MQ=255)
                                                                  cGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTcc                       >  1:4699472/1‑93 (MQ=255)
                                                                  cGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTcc                       <  2:5395449/93‑1 (MQ=255)
                                                                   gCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCg                      <  1:4111337/93‑1 (MQ=255)
                                                                    cAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCgg                     >  2:2343939/1‑93 (MQ=255)
                                                                    cAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCgg                     >  1:238987/1‑93 (MQ=255)
                                                                     aGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGa                    <  1:4235066/93‑1 (MQ=255)
                                                                     aGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGa                    <  2:6452961/93‑1 (MQ=255)
                                                                     aGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGa                    <  2:4501092/93‑1 (MQ=255)
                                                                       aCGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGAtt                  <  1:2821168/93‑1 (MQ=255)
                                                                       aCGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGAtt                  <  1:6000439/93‑1 (MQ=255)
                                                                       aCGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGAtt                  <  2:5043082/93‑1 (MQ=255)
                                                                         gCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTaa                >  1:2613404/1‑93 (MQ=255)
                                                                          cTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAAc               <  2:2660074/93‑1 (MQ=255)
                                                                           tCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACg              <  2:2749561/93‑1 (MQ=255)
                                                                           tCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACg              <  1:172968/93‑1 (MQ=255)
                                                                            cAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACgg             >  1:4487969/1‑93 (MQ=255)
                                                                            cAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATGCCGGATTAACgg             <  1:3507134/93‑1 (MQ=255)
                                                                             aGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGc            <  1:1631768/93‑1 (MQ=255)
                                                                               cTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCt          <  1:2058693/93‑1 (MQ=255)
                                                                                tAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTg         >  1:5864735/1‑93 (MQ=255)
                                                                                tAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTg         >  2:3162445/1‑93 (MQ=255)
                                                                                tAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTg         >  2:329254/1‑93 (MQ=255)
                                                                                tAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTg         >  1:3649419/1‑93 (MQ=255)
                                                                                 aaTGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGt        >  2:6276909/1‑93 (MQ=255)
                                                                                 aaTGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGt        >  1:5073790/1‑93 (MQ=255)
                                                                                 aaTGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGt        >  1:1099820/1‑93 (MQ=255)
                                                                                  aTGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTa       >  1:5265881/1‑93 (MQ=255)
                                                                                    gCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACt     >  2:3506103/1‑93 (MQ=255)
                                                                                    gCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACt     <  1:3531355/93‑1 (MQ=255)
                                                                                     ccATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTa    >  2:4096638/1‑93 (MQ=255)
                                                                                      cATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTaa   >  2:1885851/1‑93 (MQ=255)
                                                                                      cATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTaa   >  1:2024014/1‑93 (MQ=255)
                                                                                       aTAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAAc  <  1:3353024/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAAC  >  NC_000913/2637475‑2637654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATA  >  NC_000913/2637472‑2637658
                                                                                             |                                                                                             
CGGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAG                                                                                               <  SRR4302547.740108/94‑1 (MQ=60)
CGGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAG                                                                                               <  SRR4302547.6350328/94‑1 (MQ=60)
CGGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAA                                                                                               <  SRR4302547.4608342/94‑1 (MQ=60)
CGGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAA                                                                                               >  SRR4302547.2728927/1‑94 (MQ=60)
 GGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAAC                                                                                              <  SRR4302547.853373/94‑1 (MQ=60)
 GGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAAC                                                                                              <  SRR4302547.5691364/94‑1 (MQ=60)
  GGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACC                                                                                             >  SRR4302547.1567225/1‑94 (MQ=60)
  GGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACC                                                                                             <  SRR4302547.3826134/94‑1 (MQ=60)
   GCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCG                                                                                            <  SRR4302547.3760358/94‑1 (MQ=60)
    CAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGC                                                                                           <  SRR4302547.3889997/94‑1 (MQ=60)
    CAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGC                                                                                           <  SRR4302547.2587089/94‑1 (MQ=60)
    CAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGC                                                                                           >  SRR4302547.2929107/1‑94 (MQ=60)
     AAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCGCGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCA                                                                                          <  SRR4302547.2826646/94‑1 (MQ=60)
        CTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGAT                                                                                       >  SRR4302547.5134340/1‑94 (MQ=60)
         TGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATT                                                                                      >  SRR4302547.4333327/1‑94 (MQ=60)
           gtacgTAAAGTTGCCGCCGCCGTGGTGGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGT                                                                                    <  SRR4302547.647694/89‑1 (MQ=60)
           TTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGT                                                                                    <  SRR4302547.3587525/94‑1 (MQ=60)
            TTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTG                                                                                   <  SRR4302547.5562766/94‑1 (MQ=60)
            TTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTG                                                                                   >  SRR4302547.5347475/1‑94 (MQ=60)
              CTTAAAGTTGCCGCCGCCGTTGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTA                                                                                 <  SRR4302547.4257404/94‑1 (MQ=60)
               TTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTAT                                                                                >  SRR4302547.3781489/1‑94 (MQ=60)
               TTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTAT                                                                                <  SRR4302547.5090388/94‑1 (MQ=60)
               TTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTAT                                                                                >  SRR4302547.1393734/1‑94 (MQ=60)
                 AAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCA                                                                              <  SRR4302547.1373478/94‑1 (MQ=60)
                   AGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGACTGTGTATCAGA                                                                            >  SRR4302547.6465530/1‑94 (MQ=60)
                    GTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCA                                                                           >  SRR4302547.1363341/1‑94 (MQ=60)
                    GTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCA                                                                           >  SRR4302547.2802347/1‑94 (MQ=60)
                     TTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGCCGCTCAGCTAATGCCATAACCGCAGCTTGTGTATCAGAAC                                                                          >  SRR4302547.1080499/1‑94 (MQ=60)
                      TGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACC                                                                         >  SRR4302547.4049451/1‑94 (MQ=60)
                      TGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACC                                                                         <  SRR4302547.3973345/94‑1 (MQ=60)
                           CCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAG                                                                    <  SRR4302547.5626242/94‑1 (MQ=60)
                           CCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAG                                                                    <  SRR4302547.6712396/94‑1 (MQ=60)
                            CGCCGTGGTTGTTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGC                                                                   <  SRR4302547.5987108/94‑1 (MQ=60)
                            CGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGC                                                                   >  SRR4302547.6059129/1‑94 (MQ=60)
                                   GTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGG                                                            <  SRR4302547.4996826/94‑1 (MQ=60)
                                   GTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGG                                                            <  SRR4302547.4844968/94‑1 (MQ=60)
                                   GTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGG                                                            <  SRR4302547.981180/94‑1 (MQ=60)
                                   GTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGG                                                            >  SRR4302547.3644449/1‑94 (MQ=60)
                                    TTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGT                                                           >  SRR4302547.5768300/1‑94 (MQ=60)
                                    TTGGTCATCAATTTCACCACCGGTAATTGATCACGCACACGCTCAGCTAATGCCATAGCTCCAGATTGTGTATCAGCACCTGAAGACACCAGGT                                                           >  SRR4302547.5570026/1‑94 (MQ=60)
                                     TGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTA                                                          <  SRR4302547.1091770/94‑1 (MQ=60)
                                     TGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTA                                                          >  SRR4302547.1036353/1‑94 (MQ=60)
                                      GGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTAT                                                         <  SRR4302547.1985534/94‑1 (MQ=60)
                                      GGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTAT                                                         <  SRR4302547.20844/94‑1 (MQ=60)
                                         CATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATA                                                      >  SRR4302547.5971231/1‑94 (MQ=60)
                                         CATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATA                                                      >  SRR4302547.2576200/1‑94 (MQ=60)
                                          ATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATAT                                                     <  SRR4302547.148907/94‑1 (MQ=60)
                                          ATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATAT                                                     <  SRR4302547.5646517/94‑1 (MQ=60)
                                          ATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATAT                                                     <  SRR4302547.4161006/94‑1 (MQ=60)
                                           TCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATC                                                    <  SRR4302547.3572181/94‑1 (MQ=60)
                                            CAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCG                                                   <  SRR4302547.709658/94‑1 (MQ=60)
                                             AATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGA                                                  >  SRR4302547.2769659/1‑94 (MQ=60)
                                              ATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGAC                                                 <  SRR4302547.5602059/94‑1 (MQ=60)
                                                TTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAA                                               <  SRR4302547.5563210/94‑1 (MQ=60)
                                                TTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAA                                               >  SRR4302547.1982771/1‑94 (MQ=60)
                                                TTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAA                                               <  SRR4302547.4120147/94‑1 (MQ=60)
                                                 TCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAAC                                              >  SRR4302547.5994762/1‑94 (MQ=60)
                                                  CACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACA                                             <  SRR4302547.194980/94‑1 (MQ=60)
                                                   CCGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAG                                            <  SRR4302547.454703/94‑1 (MQ=60)
                                                   ACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAG                                            <  SRR4302547.4050225/94‑1 (MQ=60)
                                                    CGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGG                                           <  SRR4302547.2981350/94‑1 (MQ=60)
                                                    CGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGG                                           <  SRR4302547.1111059/94‑1 (MQ=60)
                                                     GCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGA                                          <  SRR4302547.2506192/94‑1 (MQ=60)
                                                     GCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGA                                          <  SRR4302547.2502193/94‑1 (MQ=60)
                                                      CCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGAT                                         <  SRR4302547.4766208/94‑1 (MQ=60)
                                                       CCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATC                                        <  SRR4302547.6320386/94‑1 (MQ=60)
                                                       CCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATC                                        <  SRR4302547.3113251/94‑1 (MQ=60)
                                                       CCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATC                                        <  SRR4302547.149290/94‑1 (MQ=60)
                                                       CCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATC                                        >  SRR4302547.6572839/1‑94 (MQ=60)
                                                        CGGTAATTCATCCCGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCG                                       >  SRR4302547.4750364/1‑94 (MQ=60)
                                                        CGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCG                                       <  SRR4302547.445416/94‑1 (MQ=60)
                                                         GGTAATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGG                                      >  SRR4302547.4649523/1‑94 (MQ=60)
                                                            AATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTT                                   <  SRR4302547.6431722/94‑1 (MQ=60)
                                                             ATTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTT                                  <  SRR4302547.5826135/94‑1 (MQ=60)
                                                              TTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCCCCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTA                                 <  SRR4302547.5933087/94‑1 (MQ=60)
                                                              TTCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTA                                 <  SRR4302547.969375/94‑1 (MQ=60)
                                                               TCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAA                                <  SRR4302547.895680/94‑1 (MQ=60)
                                                               TCATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAA                                <  SRR4302547.3357245/94‑1 (MQ=60)
                                                                CATCACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAA                               >  SRR4302547.15702/1‑94 (MQ=60)
                                                                    ACGCAGACGCTCAGCTAATGTCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCC                           >  SRR4302547.6605505/1‑94 (MQ=60)
                                                                    ACGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCC                           >  SRR4302547.4796809/1‑94 (MQ=60)
                                                                     CGCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCG                          <  SRR4302547.4229581/94‑1 (MQ=60)
                                                                      GCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGG                         <  SRR4302547.4194907/94‑1 (MQ=60)
                                                                      GCAGACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGG                         >  SRR4302547.243461/1‑94 (MQ=60)
                                                                        AGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGAT                       <  SRR4302547.4321652/94‑1 (MQ=60)
                                                                         GACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATT                      <  SRR4302547.6089257/94‑1 (MQ=60)
                                                                          ACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTA                     <  SRR4302547.6124958/94‑1 (MQ=60)
                                                                          ACGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTA                     <  SRR4302547.2877601/94‑1 (MQ=60)
                                                                           CGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAA                    >  SRR4302547.758601/1‑94 (MQ=60)
                                                                           CGCTCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAA                    >  SRR4302547.2666071/1‑94 (MQ=60)
                                                                             CTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACG                  >  SRR4302547.1715243/1‑94 (MQ=60)
                                                                              TCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGG                 >  SRR4302547.4580666/1‑94 (MQ=60)
                                                                              TCAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGG                 <  SRR4302547.176138/94‑1 (MQ=60)
                                                                               CAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGC                >  SRR4302547.4629917/1‑94 (MQ=60)
                                                                               CAGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATGCCGGATTAACGGC                <  SRR4302547.3578715/94‑1 (MQ=60)
                                                                                AGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCC               <  SRR4302547.1664068/94‑1 (MQ=60)
                                                                                AGCTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCC               >  SRR4302547.18832/1‑94 (MQ=60)
                                                                                  CTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             <  SRR4302547.686474/94‑1 (MQ=60)
                                                                                  CTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             >  SRR4302547.5986115/1‑94 (MQ=60)
                                                                                  CTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             <  SRR4302547.2292094/94‑1 (MQ=60)
                                                                                  CTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             >  SRR4302547.3723856/1‑94 (MQ=60)
                                                                                  CTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             >  SRR4302547.654627/1‑94 (MQ=60)
                                                                                  CTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             <  SRR4302547.616278/94‑1 (MQ=60)
                                                                                  CTAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTG             <  SRR4302547.2099774/94‑1 (MQ=60)
                                                                                   TAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGT            <  SRR4302547.2506286/94‑1 (MQ=60)
                                                                                   TAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGT            >  SRR4302547.1121911/1‑94 (MQ=60)
                                                                                   TAATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGT            >  SRR4302547.5178467/1‑94 (MQ=60)
                                                                                    AATGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTA           >  SRR4302547.5374330/1‑94 (MQ=60)
                                                                                      TGCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACT         >  SRR4302547.42998/1‑94 (MQ=60)
                                                                                       GCCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTA        <  SRR4302547.3603458/94‑1 (MQ=60)
                                                                                        CCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAA       <  SRR4302547.4704036/94‑1 (MQ=60)
                                                                                        CCATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAA       >  SRR4302547.2064321/1‑94 (MQ=60)
                                                                                          ATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACA     <  SRR4302547.1082553/94‑1 (MQ=60)
                                                                                          ATAACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACA     <  SRR4302547.3421019/94‑1 (MQ=60)
                                                                                            AACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAAT   <  SRR4302547.1469695/94‑1 (MQ=60)
                                                                                            AACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAAT   >  SRR4302547.113069/1‑94 (MQ=60)
                                                                                            AACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAAT   >  SRR4302547.6218896/1‑94 (MQ=60)
                                                                                             ACCGCAGATTTTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATA  <  SRR4302547.1522487/94‑1 (MQ=60)
                                                                                             ACCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATA  >  SRR4302547.514104/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGGCAAACTGTTTCTTAAAGTTGCCGCCGCCGTGGTTGGTCATCAATTTCACGCCCGGTAATTCATCACGCAGACGCTCAGCTAATGCCATAGCCGCAGATTGTGTATCAGCACCTGAAGCCACCAGGTATATATCGACAACAGGATCGGCTTTAAATTCCGGATTAACGGCCTGTACTAACAATA  >  NC_000913/2637472‑2637658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑