Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,647,829 T→C T827A (ACG→GCG)  b2520 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,8290TC84.5% 568.7 / 91.0 226T827A (ACG→GCG) b2520hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/20);  new base C (91/100);  total (106/120)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

Only 100 of 229 total aligned reads displayed.

GGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACC  >  NC_000913/2647739‑2647918
                                                                                          |                                                                                         
ggTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCAc                                                                                         >  2:3526946/1‑93 (MQ=255)
 gTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACt                                                                                        >  2:4089672/1‑93 (MQ=255)
  ttttCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACtt                                                                                       >  2:89203/1‑93 (MQ=255)
   tttCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTa                                                                                      <  1:2893378/93‑1 (MQ=255)
   tttCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTa                                                                                      >  1:4499674/1‑93 (MQ=255)
   tttCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTa                                                                                      >  1:1610226/1‑93 (MQ=255)
      cATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAAt                                                                                   <  1:4865958/93‑1 (MQ=255)
       aTCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATa                                                                                  >  2:2383636/1‑93 (MQ=255)
        tCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATAc                                                                                 >  1:443455/1‑93 (MQ=255)
        ccgcgAAGCGGCAGATGCAACTCACCACCCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATAc                                                                                 <  1:4098537/92‑1 (MQ=255)
         cGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACa                                                                                <  2:6624937/93‑1 (MQ=255)
         cGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACa                                                                                <  2:3522614/93‑1 (MQ=255)
          gCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAg                                                                               <  1:5169310/93‑1 (MQ=255)
          gCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAg                                                                               >  1:744285/1‑93 (MQ=255)
            cAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGAt                                                                             <  2:5049884/93‑1 (MQ=255)
            cAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGAt                                                                             >  2:5963845/1‑93 (MQ=255)
             aaGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATc                                                                            <  2:3592995/93‑1 (MQ=255)
             aaGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATc                                                                            <  2:3241134/93‑1 (MQ=255)
             aaGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATc                                                                            <  1:5043593/93‑1 (MQ=255)
              aGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCa                                                                           <  2:5668309/93‑1 (MQ=255)
               gCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGGGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATTCAGATCAt                                                                          <  1:6503799/93‑1 (MQ=255)
                 ggCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGa                                                                        >  2:3961917/1‑93 (MQ=255)
                 ggCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGa                                                                        <  1:456841/93‑1 (MQ=255)
                    aGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGa                                                                     >  2:6464125/1‑93 (MQ=255)
                     gATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCTTGACGAt                                                                    <  2:2908977/93‑1 (MQ=255)
                     gATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGAt                                                                    >  2:5788486/1‑93 (MQ=255)
                      aTGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGAtt                                                                   <  1:6234995/93‑1 (MQ=255)
                       tGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTc                                                                  >  1:579619/1‑93 (MQ=255)
                       tGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTc                                                                  <  1:589454/93‑1 (MQ=255)
                        gCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTcc                                                                 <  2:6360149/93‑1 (MQ=255)
                        gCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTcc                                                                 >  1:2562902/1‑93 (MQ=255)
                         cAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCa                                                                >  2:2446552/1‑93 (MQ=255)
                           acacacCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAgg                                                              <  2:6414688/93‑1 (MQ=255)
                            cacacCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGt                                                             <  1:1427029/93‑1 (MQ=255)
                              cacCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGttt                                                           <  2:713033/93‑1 (MQ=255)
                               cccAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATCCCAGGtttt                                                          <  2:1156387/92‑1 (MQ=255)
                               acCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGtttt                                                          >  1:4985546/1‑93 (MQ=255)
                                  aaCCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATc                                                       >  1:938509/1‑93 (MQ=255)
                                   aCCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCg                                                      >  1:2678393/1‑93 (MQ=255)
                                   aCCGCGCGTTTTGGCGGCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCg                                                      <  1:3911435/93‑1 (MQ=255)
                                    ccGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGa                                                     >  1:1992280/1‑93 (MQ=255)
                                     cgcgcgTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATNATGACGATTCCAGGTTTTATCGAc                                                    >  1:1281413/1‑93 (MQ=255)
                                     cgcgcgTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGAc                                                    >  1:1087210/1‑93 (MQ=255)
                                      gcgcgTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGAcc                                                   >  2:4634842/1‑93 (MQ=255)
                                      gcgcgTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGAcc                                                   <  2:1666628/93‑1 (MQ=255)
                                       cgcgTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCg                                                  <  1:222015/93‑1 (MQ=255)
                                        gcgTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCgg                                                 >  2:2069644/1‑93 (MQ=255)
                                         cgTTTTGGCGGCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGa                                                <  1:2046187/93‑1 (MQ=255)
                                          gTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGaa                                               <  2:3818675/93‑1 (MQ=255)
                                           ttttGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAAt                                              <  1:1562594/93‑1 (MQ=255)
                                           ttttGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAAt                                              <  1:2784951/93‑1 (MQ=255)
                                           ttttGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAAt                                              <  1:530788/93‑1 (MQ=255)
                                            tttGGCGGCGCGGAACGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATc                                             <  2:1272602/93‑1 (MQ=255)
                                              tGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGt                                           <  1:3648964/93‑1 (MQ=255)
                                              tGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGt                                           <  1:5257802/93‑1 (MQ=255)
                                               ggCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTc                                          <  2:5446293/93‑1 (MQ=255)
                                               ggCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTc                                          <  1:2402316/93‑1 (MQ=255)
                                               ggCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTc                                          <  2:3922580/93‑1 (MQ=255)
                                               ggCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTc                                          <  2:1728907/93‑1 (MQ=255)
                                                gCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCa                                         <  1:794882/93‑1 (MQ=255)
                                                gCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCa                                         >  2:1130617/1‑93 (MQ=255)
                                                     gcgGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATcc                                    <  1:5642405/93‑1 (MQ=255)
                                                     gcgGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATcc                                    >  1:3100316/1‑93 (MQ=255)
                                                     gcgGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATcc                                    <  2:4482265/93‑1 (MQ=255)
                                                       ggAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAg                                  <  1:4618524/93‑1 (MQ=255)
                                                          gCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGccc                               <  2:1847944/93‑1 (MQ=255)
                                                          gCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGccc                               <  2:2160712/93‑1 (MQ=255)
                                                           cGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGccat                              <  1:6549908/93‑3 (MQ=255)
                                                           cGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCt                              >  2:4255337/1‑93 (MQ=255)
                                                           cGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCt                              <  1:1692691/93‑1 (MQ=255)
                                                            gagaTTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCtt                             <  1:1021469/93‑1 (MQ=255)
                                                             agaTTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTg                            >  1:2713014/1‑93 (MQ=255)
                                                              gaTTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGa                           >  2:4506056/1‑93 (MQ=255)
                                                              gaTTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGa                           >  1:3237818/1‑93 (MQ=255)
                                                                 ttATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGcc                        >  1:967327/1‑93 (MQ=255)
                                                                  tATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGccc                       <  2:3036074/93‑1 (MQ=255)
                                                                   aTCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCg                      >  2:4919907/1‑93 (MQ=255)
                                                                   aTCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCg                      >  1:3431323/1‑93 (MQ=255)
                                                                   aTCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCg                      >  2:1421256/1‑93 (MQ=255)
                                                                     cGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGaa                    <  1:1893257/93‑1 (MQ=255)
                                                                     cGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGaa                    <  1:4029863/93‑1 (MQ=255)
                                                                      gCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAAc                   >  2:2849939/1‑93 (MQ=255)
                                                                      gCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAAc                   <  2:6085695/93‑1 (MQ=255)
                                                                       ccAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACa                  <  1:5466279/93‑1 (MQ=255)
                                                                       ccAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACa                  >  2:4745219/1‑93 (MQ=255)
                                                                         aGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATc                >  1:3302817/1‑93 (MQ=255)
                                                                         aGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATc                >  2:4818619/1‑93 (MQ=255)
                                                                         aGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATc                <  2:4124398/93‑1 (MQ=255)
                                                                         aGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATc                <  1:3535473/93‑1 (MQ=255)
                                                                         aGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATc                >  2:2248795/1‑93 (MQ=255)
                                                                           gACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCaa              >  2:2089438/1‑93 (MQ=255)
                                                                            aCGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAAt             <  2:5263597/93‑1 (MQ=255)
                                                                             cGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAAtc            >  1:6009787/1‑93 (MQ=255)
                                                                              gTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAAtct           <  2:3743386/93‑1 (MQ=255)
                                                                               tACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAAtctc          <  1:5596137/93‑1 (MQ=255)
                                                                                accaccAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATctct         <  2:3620458/93‑1 (MQ=255)
                                                                                    ccaGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGcc     >  2:3076025/1‑93 (MQ=255)
                                                                                      aGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGTATCGTCAGATTCAGCCCTTGAGACCGAACATCAATCTCTTGccac   >  1:6643873/1‑93 (MQ=255)
                                                                                       gcgcACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGccacc  <  1:2303257/93‑1 (MQ=255)
                                                                                       gcgcACTCAAATACAGACCATGACGATTCCAGGTTCTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGccacc  <  1:3619422/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACC  >  NC_000913/2647739‑2647918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAG  >  NC_000913/2647738‑2647922
                                                                                           |                                                                                             
CGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCAC                                                                                             <  SRR4302547.5882526/94‑1 (MQ=60)
CGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCAC                                                                                             <  SRR4302547.6060385/94‑1 (MQ=60)
 GGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACT                                                                                            <  SRR4302547.2330702/94‑1 (MQ=60)
   TTTTCATCGCCACGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTA                                                                                          >  SRR4302547.1101060/1‑94 (MQ=60)
   TTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTA                                                                                          >  SRR4302547.4592623/1‑94 (MQ=60)
   TTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTA                                                                                          >  SRR4302547.1642128/1‑94 (MQ=60)
    TTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAA                                                                                         <  SRR4302547.2951088/94‑1 (MQ=60)
     TTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAA                                                                                        <  SRR4302547.5391788/94‑1 (MQ=60)
       CATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATA                                                                                      <  SRR4302547.4966796/94‑1 (MQ=60)
        ATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATAC                                                                                     >  SRR4302547.2347984/1‑94 (MQ=60)
        ATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATAC                                                                                     >  SRR4302547.451713/1‑94 (MQ=60)
         ccgcgAAGCGGCAGATGCAACTCACCACCCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACA                                                                                    <  SRR4302547.4181889/89‑1 (MQ=60)
         TCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACA                                                                                    <  SRR4302547.3682411/94‑1 (MQ=60)
          CGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAG                                                                                   >  SRR4302547.758299/1‑94 (MQ=60)
          CGCCAAGCGGCAGATGCAACACACCAACCGCGCGTGTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAG                                                                                   <  SRR4302547.1627716/94‑1 (MQ=60)
           GCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGA                                                                                  <  SRR4302547.5275747/94‑1 (MQ=60)
            CCAAGCGGCAGATGCAACACACCACCCGCGCGTTTTGCCGGCGCGGAGCGAGATTTATGGCCAGGACGTACCACCAGCGCACTTAAATACAGAT                                                                                 <  SRR4302547.4394582/94‑1 (MQ=60)
             CAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATC                                                                                >  SRR4302547.48407/1‑94 (MQ=60)
              AAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCA                                                                               <  SRR4302547.5147737/94‑1 (MQ=60)
                GCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGGGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATTCAGATCATG                                                                             <  SRR4302547.6639182/94‑1 (MQ=60)
                  GGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGAC                                                                           <  SRR4302547.465293/94‑1 (MQ=60)
                  GGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGAC                                                                           >  SRR4302547.6069279/1‑94 (MQ=60)
                   GCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACG                                                                          <  SRR4302547.1984263/94‑1 (MQ=60)
                   GCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACG                                                                          <  SRR4302547.2673494/94‑1 (MQ=60)
                     AGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGAT                                                                        >  SRR4302547.4193584/1‑94 (MQ=60)
                      GATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATT                                                                       <  SRR4302547.452781/94‑1 (MQ=60)
                      GATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATT                                                                       >  SRR4302547.2492814/1‑94 (MQ=60)
                       ATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTC                                                                      >  SRR4302547.590013/1‑94 (MQ=60)
                       ATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTC                                                                      >  SRR4302547.2737785/1‑94 (MQ=60)
                       ATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTC                                                                      <  SRR4302547.6364524/94‑1 (MQ=60)
                        TGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCC                                                                     <  SRR4302547.600130/94‑1 (MQ=60)
                        TGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCC                                                                     >  SRR4302547.5142826/1‑94 (MQ=60)
                        TGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCC                                                                     >  SRR4302547.2614681/1‑94 (MQ=60)
                         GCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCA                                                                    <  SRR4302547.2787808/94‑1 (MQ=60)
                          CAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAG                                                                   >  SRR4302547.2696779/1‑94 (MQ=60)
                           AACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGG                                                                  <  SRR4302547.3873957/94‑1 (MQ=60)
                            ACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGT                                                                 <  SRR4302547.2867497/94‑1 (MQ=60)
                             CACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTT                                                                <  SRR4302547.1455582/94‑1 (MQ=60)
                             CACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTT                                                                >  SRR4302547.2432420/1‑94 (MQ=60)
                              ACACCAACCGCGCGTTTTGGCGTCGCGGACCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTT                                                               <  SRR4302547.6667974/94‑1 (MQ=60)
                               CACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTT                                                              >  SRR4302547.5088631/1‑94 (MQ=60)
                               CACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTT                                                              <  SRR4302547.1484892/94‑1 (MQ=60)
                                ACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTA                                                             <  SRR4302547.4833904/94‑1 (MQ=60)
                                ACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTA                                                             <  SRR4302547.985344/94‑1 (MQ=60)
                                  CAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATC                                                           >  SRR4302547.956679/1‑94 (MQ=60)
                                   AACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATTGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCG                                                          >  SRR4302547.3409073/1‑94 (MQ=60)
                                   AACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCG                                                          <  SRR4302547.5343371/94‑1 (MQ=60)
                                   AACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCG                                                          >  SRR4302547.2732182/1‑94 (MQ=60)
                                    ACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGA                                                         >  SRR4302547.2031866/1‑94 (MQ=60)
                                    ACCGCGCGTTTTGGCGGCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGA                                                         <  SRR4302547.3990973/94‑1 (MQ=60)
                                     CCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATNATGACGATTCCAGGTTTTATCGAC                                                        >  SRR4302547.1307233/1‑94 (MQ=60)
                                     CCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGAC                                                        >  SRR4302547.1108954/1‑94 (MQ=60)
                                      CGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACC                                                       >  SRR4302547.773216/1‑94 (MQ=60)
                                      CGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACC                                                       >  SRR4302547.2059368/1‑94 (MQ=60)
                                        CGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGG                                                     <  SRR4302547.226193/94‑1 (MQ=60)
                                          CGTTTTGGCGGCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAA                                                   <  SRR4302547.2087005/94‑1 (MQ=60)
                                           GTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAAT                                                  <  SRR4302547.1848340/94‑1 (MQ=60)
                                           GTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAAT                                                  <  SRR4302547.671871/94‑1 (MQ=60)
                                            TTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATC                                                 <  SRR4302547.1593719/94‑1 (MQ=60)
                                            TTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATC                                                 <  SRR4302547.2840732/94‑1 (MQ=60)
                                            TTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATC                                                 <  SRR4302547.540375/94‑1 (MQ=60)
                                            TTTTGGCGTCGCGAAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTTCAGGTTTTATCGACCGGAATC                                                 >  SRR4302547.5438626/1‑94 (MQ=60)
                                             TTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCG                                                <  SRR4302547.6136607/94‑1 (MQ=60)
                                               TGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTC                                              <  SRR4302547.3723391/94‑1 (MQ=60)
                                               TGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTC                                              <  SRR4302547.5366071/94‑1 (MQ=60)
                                                GGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCA                                             <  SRR4302547.6416187/94‑1 (MQ=60)
                                                GGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCA                                             <  SRR4302547.2450970/94‑1 (MQ=60)
                                                 GCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAG                                            <  SRR4302547.809925/94‑1 (MQ=60)
                                                    TCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATC                                         >  SRR4302547.5940282/1‑94 (MQ=60)
                                                    TCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATC                                         >  SRR4302547.3278757/1‑94 (MQ=60)
                                                     CGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCC                                        >  SRR4302547.3161722/1‑94 (MQ=60)
                                                     CGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCC                                        >  SRR4302547.4707128/1‑94 (MQ=60)
                                                      GCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCA                                       >  SRR4302547.3455732/1‑94 (MQ=60)
                                                      GCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATTCA                                       >  SRR4302547.1415665/1‑94 (MQ=60)
                                                      GCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCA                                       <  SRR4302547.5758879/94‑1 (MQ=60)
                                                       CGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAG                                      >  SRR4302547.4252347/1‑94 (MQ=60)
                                                        GGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGC                                     >  SRR4302547.720183/1‑94 (MQ=60)
                                                        GGAGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGC                                     <  SRR4302547.4714154/94‑1 (MQ=60)
                                                          AGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCC                                   <  SRR4302547.5450268/94‑1 (MQ=60)
                                                          AGCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCC                                   <  SRR4302547.4650491/94‑1 (MQ=60)
                                                           GCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCT                                  <  SRR4302547.5026390/94‑1 (MQ=60)
                                                           GCGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCT                                  <  SRR4302547.3533577/94‑1 (MQ=60)
                                                            CGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTT                                 >  SRR4302547.4179064/1‑94 (MQ=60)
                                                            CGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTT                                 <  SRR4302547.1725991/94‑1 (MQ=60)
                                                            CGAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCATT                                 <  SRR4302547.6686213/94‑1 (MQ=60)
                                                             GAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTG                                >  SRR4302547.2767474/1‑94 (MQ=60)
                                                             GAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTG                                >  SRR4302547.3360904/1‑94 (MQ=60)
                                                             GAGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTG                                <  SRR4302547.1041495/94‑1 (MQ=60)
                                                              AGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGA                               >  SRR4302547.3302445/1‑94 (MQ=60)
                                                              AGATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGA                               <  SRR4302547.582700/94‑1 (MQ=60)
                                                               GATTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAG                              <  SRR4302547.5201681/94‑1 (MQ=60)
                                                                 TTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCC                            >  SRR4302547.6377088/1‑94 (MQ=60)
                                                                 TTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCC                            >  SRR4302547.986112/1‑94 (MQ=60)
                                                                 TTTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCC                            <  SRR4302547.3893509/94‑1 (MQ=60)
                                                                  TTATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGGCCTTGAGCCC                           >  SRR4302547.121799/1‑94 (MQ=60)
                                                                   TATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATTGACCGGAATCGTCAGATCCAGCCCTTGAGCCCG                          >  SRR4302547.3057379/1‑94 (MQ=60)
                                                                   TATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCG                          <  SRR4302547.5265167/94‑1 (MQ=60)
                                                                   TATCGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCG                          >  SRR4302547.3501244/1‑94 (MQ=60)
                                                                      CGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAAC                       <  SRR4302547.1930671/94‑1 (MQ=60)
                                                                      CGCCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAAC                       <  SRR4302547.4111726/94‑1 (MQ=60)
                                                                        CCAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACAT                     <  SRR4302547.5579201/94‑1 (MQ=60)
                                                                         CAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATC                    >  SRR4302547.1680517/1‑94 (MQ=60)
                                                                         CAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATC                    >  SRR4302547.3369317/1‑94 (MQ=60)
                                                                         AAGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTCTCGACCGGATTCGTCAGATCCAGCCCTTGAGCCCGAACATC                    >  SRR4302547.3253206/1‑94 (MQ=60)
                                                                          AGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCA                   <  SRR4302547.3607657/94‑1 (MQ=60)
                                                                          AGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCA                   >  SRR4302547.571067/1‑94 (MQ=60)
                                                                          AGGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCA                   >  SRR4302547.5947681/1‑94 (MQ=60)
                                                                           GGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAA                  >  SRR4302547.3715215/1‑94 (MQ=60)
                                                                           GGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAA                  <  SRR4302547.5696328/94‑1 (MQ=60)
                                                                           GGACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAA                  >  SRR4302547.1875583/1‑94 (MQ=60)
                                                                             ACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATC                >  SRR4302547.775548/1‑94 (MQ=60)
                                                                             ACGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATC                >  SRR4302547.6134522/1‑94 (MQ=60)
                                                                              CGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCT               <  SRR4302547.853208/94‑1 (MQ=60)
                                                                              CGTACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCT               <  SRR4302547.2189088/94‑1 (MQ=60)
                                                                                TACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCT             <  SRR4302547.3059863/94‑1 (MQ=60)
                                                                                TACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCT             <  SRR4302547.1516472/94‑1 (MQ=60)
                                                                                TACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCT             <  SRR4302547.5711583/94‑1 (MQ=60)
                                                                                 ACCACCAGCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTT            <  SRR4302547.4011757/94‑1 (MQ=60)
                                                                                      CAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGTATCGTCAGATTCAGCCCTTGAGACCGAACATCAATCTCTTGCCAC       >  SRR4302547.6782199/1‑94 (MQ=60)
                                                                                        GCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCA     <  SRR4302547.2349933/94‑1 (MQ=60)
                                                                                        GCGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCA     >  SRR4302547.5363863/1‑94 (MQ=60)
                                                                                        GCGCACTCAAATACAGACCATGACGATTCCAGGTTCTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCA     <  SRR4302547.3693256/94‑1 (MQ=60)
                                                                                         CGCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCAC    >  SRR4302547.1191979/1‑94 (MQ=60)
                                                                                          GCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACA   >  SRR4302547.2535000/1‑94 (MQ=60)
                                                                                          GCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACA   >  SRR4302547.1257333/1‑94 (MQ=60)
                                                                                          GCACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACA   <  SRR4302547.3843599/94‑1 (MQ=60)
                                                                                           CACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAG  <  SRR4302547.6598726/94‑1 (MQ=60)
                                                                                           |                                                                                             
CGGTTTTCATCGCCAAGCGGCAGATGCAACACACCAACCGCGCGTTTTGGCGTCGCGGAGCGAGATTTATCGCCAGGACGTACCACCAGCGTACTTAAATACAGATCATGACGATTCCAGGTTTTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAG  >  NC_000913/2647738‑2647922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑